pycom03g12530

Phytochromobilin ferredoxin oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005084
Physical Location & Seq
Forward (+)
13136155 .. 13141300
5146 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1164 bp
ATGAAGCATACGAGGTGGGGTCCATGTAGATTTCTGCTCACTACCACTTCTGTATTACCGGATAGAAGGGGTTTATTGAATTTTTATGGTTGCTGTTGGTGGTTGATTGCCTCAGTGCAAGTTTCTCTCTCTCTCTCTCTCTCTCTCTCTTCAATGGAGTGTTCTTCTTCTCTAAGGTTTTGCATACCCAAAAAACCTTTGCCATCAATCAGCACTGTATTGTTCTCTAGTATTTGCAGCAGCAGCAGCAGGAAGCAGCAGAGACTCATTTCCCAACAACTCTCTGCAGTTTCTTACAAGAAATTCATTCACTTTGCTTTGGAGGAAACCAAACTCCACACCCATCTGGAACCTTCTCCCTTGCAGTCCTTTGTACGTCTTCAGGAAAGATATAATTCCAGGATTTCAATGGATGGTAAAGCAGATATTCAAATGTTATCATTCGAAGCTACTAAAATCAGACATCTACGAAGCTTATGCATTGAGAGCCAAACAATGCAGATTTTGGATTTTGCTGTCTTCCCAGAACCTGAATTTGATATGCCCATATTTTGTGCCAACTTCTTCACCAGTGCAAATATAAACATAGTCGTGTTGGACCTCAATCCGTTGCACGATGTAATCAGTCAAAGGCATTACAGAGACAAATACTATAAAAGCCTAATACCTCTTGGTGTTAAATATTCTGAGCTTTTACCCTGGGGAGGAAAGCTTACGAGCGAGTCACTTAAATTCTTTTCACCAATTGTGATATGGACAAGATTTACGTCAAGCCCCAAAAATTATGATGTTTTATATTCTGCATTCATGGATTATTACAAGGCATGGCTTGCGCTGATGGACCAAGCAGTAGTTGAGACAAATGCCTCCCAAATTATATATAACCGTGAAGCGCAACATAGATATCTAACATGGAGAGCAGAGAAGGATCCCGGACACGCACTACTGAAAAAGTTGATTGGGGAGACACAAGCTAAGGACTTGCTGGTGAACTTCCTCTTTAATGGAATTGATGAGCTAGGAAGCAAAACTTTCCTCGATTACTTTCCAGAGTACGGCTATGAGGATGGGACTGTAAACCAGAGTCGCAGTATGATAGGGAAATCTTTTGAAAGTCGTCCCTGGGATACAAAAGGAGAATTTATTAGTAACACAGACTTTTGA

Protein Analysis

388

Amino Acids

44.63

Weight (kDa)

8.64

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe_bilin_red PF05996 125 - 335 4.8e-54 Ferredoxin-dependent bilin reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 923, 936
AcsI RAATTY 5 cut(s) 79, 302, 533, 731, 1139
AcuI CTGAAG 1 cut(s) 365
AfaI GTAC 2 cut(s) 375, 1055
AfiI CCNNNNNNNGG 2 cut(s) 704, 1055
AgsI TTSAA 5 cut(s) 79, 153, 408, 431, 1112
AjnI CCWGG 3 cut(s) 398, 698, 1121
AluBI AGCT 6 cut(s) 449, 474, 691, 712, 974, 1018
AluI AGCT 6 cut(s) 449, 474, 691, 712, 974, 1018
Alw26I GTCTC 4 cut(s) 256, 636, 851, 959
AlwI GGATC 2 cut(s) 923, 936
AlwNI CAGNNNCTG 1 cut(s) 530
ApeKI GCWGC 5 cut(s) 237, 240, 243, 246, 256
ApoI RAATTY 5 cut(s) 79, 302, 533, 731, 1139
AspLEI GCGC 2 cut(s) 835, 895
AspS9I GGNCC 3 cut(s) 20, 598, 841
AsuC2I CCSGG 1 cut(s) 933
AsuHPI GGTGA 3 cut(s) 559, 732, 1000
AsuII TTCGAA 1 cut(s) 444
AvaII GGWCC 3 cut(s) 20, 598, 841
BamHI GGATCC 1 cut(s) 928
BbsI GAAGAC 2 cut(s) 371, 511
BbvI GCAGC 5 cut(s) 249, 252, 255, 258, 268
BccI CCATC 5 cut(s) 211, 351, 407, 832, 1061
BceAI ACGGC 1 cut(s) 1072
BcgI CGANNNNNNTGC 2 cut(s) 459, 493
BciT130I CCWGG 3 cut(s) 400, 700, 1123
BciVI GTATCC 1 cut(s) 1120
BcnI CCSGG 1 cut(s) 933
BcoDI GTCTC 4 cut(s) 256, 636, 851, 959
BfaI CTAG 2 cut(s) 228, 1019
BfmI CTRYAG 1 cut(s) 285
BfuI GTATCC 1 cut(s) 1120
BisI GCNGC 5 cut(s) 238, 241, 244, 247, 257
BlsI GCNGC 5 cut(s) 239, 242, 245, 248, 258
Bme1390I CCNGG 4 cut(s) 400, 700, 933, 1123
Bme18I GGWCC 3 cut(s) 20, 598, 841
BmgT120I GGNCC 3 cut(s) 20, 598, 841
BmiI GGNNCC 3 cut(s) 21, 351, 930
BmrFI CCNGG 4 cut(s) 400, 700, 933, 1123
BpiI GAAGAC 2 cut(s) 371, 511
Bpu10I CCTNAGC 1 cut(s) 975
Bpu14I TTCGAA 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 933
BsaJI CCNNGG 4 cut(s) 698, 699, 1121, 1122
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 2 cut(s) 704, 1055
Bse1I ACTGG 1 cut(s) 570
BseBI CCWGG 3 cut(s) 400, 700, 1123
BseDI CCNNGG 4 cut(s) 698, 699, 1121, 1122
BseGI GGATG 2 cut(s) 418, 1072
BseLI CCNNNNNNNGG 2 cut(s) 704, 1055
BseMII CTCAG 2 cut(s) 126, 678
BseNI ACTGG 1 cut(s) 570
BseXI GCAGC 5 cut(s) 249, 252, 255, 258, 268
BsiSI CCGG 2 cut(s) 59, 933
BslFI GGGAC 2 cut(s) 1084, 1104
BslI CCNNNNNNNGG 2 cut(s) 704, 1055
BsmAI GTCTC 4 cut(s) 256, 636, 851, 959
BsmFI GGGAC 2 cut(s) 1084, 1104
BsmI GAATGC 1 cut(s) 803
Bsp119I TTCGAA 1 cut(s) 444
Bsp143I GATC 1 cut(s) 928
BspCNI CTCAG 2 cut(s) 125, 679
BspLI GGNNCC 3 cut(s) 21, 351, 930
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 2 cut(s) 923, 936
BspT104I TTCGAA 1 cut(s) 444
BsrI ACTGG 1 cut(s) 570
BssECI CCNNGG 4 cut(s) 698, 699, 1121, 1122
BssMI GATC 1 cut(s) 928
Bst2UI CCWGG 3 cut(s) 400, 700, 1123
Bst4CI ACNGT 3 cut(s) 217, 887, 1075
Bst6I CTCTTC 1 cut(s) 154
BstAPI GCANNNNNTGC 1 cut(s) 830
BstBI TTCGAA 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 831
BstDEI CTNAG 4 cut(s) 112, 173, 687, 975
BstF5I GGATG 2 cut(s) 418, 1072
BstHHI GCGC 2 cut(s) 835, 895
BstKTI GATC 1 cut(s) 931
BstMAI GTCTC 4 cut(s) 256, 636, 851, 959
BstMBI GATC 1 cut(s) 928
BstMWI GCNNNNNNNGC 4 cut(s) 243, 246, 486, 830
BstNI CCWGG 3 cut(s) 400, 700, 1123
BstSCI CCNGG 4 cut(s) 398, 698, 931, 1121
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 5 cut(s) 249, 252, 255, 258, 268
BstV2I GAAGAC 2 cut(s) 371, 511
BstX2I RGATCY 1 cut(s) 928
BstYI RGATCY 1 cut(s) 928
BsuI GTATCC 1 cut(s) 1120
BtsCI GGATG 2 cut(s) 418, 1072
BtsIMutI CAGTG 3 cut(s) 120, 213, 577
Cac8I GCNNGC 1 cut(s) 831
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 2 cut(s) 835, 895
Cfr13I GGNCC 3 cut(s) 20, 598, 841
Csp6I GTAC 2 cut(s) 374, 1054
CviAII CATG 4 cut(s) 24, 808, 825, 912
CviQI GTAC 2 cut(s) 374, 1054
DdeI CTNAG 4 cut(s) 112, 173, 687, 975
DpnI GATC 1 cut(s) 930
DpnII GATC 1 cut(s) 928
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Eco32I GATATC 1 cut(s) 905
Eco47I GGWCC 3 cut(s) 20, 598, 841
Eco57I CTGAAG 1 cut(s) 365
EcoRII CCWGG 3 cut(s) 398, 698, 1121
EcoRV GATATC 1 cut(s) 905
EcoT22I ATGCAT 1 cut(s) 482
FaeI CATG 4 cut(s) 27, 811, 828, 915
FalI AAGNNNNNCTT 2 cut(s) 1015, 1047
FaqI GGGAC 2 cut(s) 1084, 1104
FatI CATG 4 cut(s) 23, 807, 824, 911
Fnu4HI GCNGC 5 cut(s) 238, 241, 244, 247, 257
FokI GGATG 2 cut(s) 425, 1079
Fsp4HI GCNGC 5 cut(s) 238, 241, 244, 247, 257
FspBI CTAG 2 cut(s) 228, 1019
GlaI GCGC 2 cut(s) 834, 894
GluI GCNGC 5 cut(s) 238, 241, 244, 247, 257
HapII CCGG 2 cut(s) 59, 933
HhaI GCGC 2 cut(s) 835, 895
Hin1II CATG 4 cut(s) 27, 811, 828, 915
Hin6I GCGC 2 cut(s) 833, 893
HinP1I GCGC 2 cut(s) 833, 893
HindIII AAGCTT 2 cut(s) 472, 710
HinfI GANTC 3 cut(s) 264, 722, 1084
HpaII CCGG 2 cut(s) 59, 933
HphI GGTGA 3 cut(s) 559, 732, 1000
Hpy166II GTNNAC 2 cut(s) 991, 1078
Hpy188I TCNGA 2 cut(s) 461, 688
Hpy188III TCNNGA 3 cut(s) 347, 383, 1049
Hpy8I GTNNAC 2 cut(s) 991, 1078
HpyAV CCTTC 3 cut(s) 60, 363, 919
HpyCH4III ACNGT 3 cut(s) 217, 887, 1075
HpyCH4IV ACGT 2 cut(s) 376, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 246, 486, 830
HpyF3I CTNAG 4 cut(s) 112, 173, 687, 975
HpySE526I ACGT 2 cut(s) 376, 767
Hsp92II CATG 4 cut(s) 27, 811, 828, 915
HspAI GCGC 2 cut(s) 833, 893
Kzo9I GATC 1 cut(s) 928
Lsp1109I GCAGC 5 cut(s) 249, 252, 255, 258, 268
MaeI CTAG 2 cut(s) 228, 1019
MaeII ACGT 2 cut(s) 376, 767
MaeIII GTNAC 2 cut(s) 723, 1148
MalI GATC 1 cut(s) 930
MboI GATC 1 cut(s) 928
MboII GAAGA 6 cut(s) 141, 156, 159, 371, 511, 556
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 1 cut(s) 928
MlyI GAGTC 3 cut(s) 258, 731, 1093
MmeI TCCRAC 1 cut(s) 576
Mph1103I ATGCAT 1 cut(s) 482
MseI TTAA 3 cut(s) 678, 729, 1002
MslI CAYNNNNRTG 1 cut(s) 590
MspI CCGG 2 cut(s) 59, 933
MspR9I CCNGG 4 cut(s) 400, 700, 933, 1123
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 803
MvaI CCWGG 3 cut(s) 400, 700, 1123
MwoI GCNNNNNNNGC 4 cut(s) 243, 246, 486, 830
NciI CCSGG 1 cut(s) 933
NdeII GATC 1 cut(s) 928
NlaIII CATG 4 cut(s) 27, 811, 828, 915
NlaIV GGNNCC 3 cut(s) 21, 351, 930
NmuCI GTSAC 1 cut(s) 723
NsiI ATGCAT 1 cut(s) 482
NspV TTCGAA 1 cut(s) 444
PasI CCCWGGG 2 cut(s) 699, 1122
PctI GAATGC 1 cut(s) 803
PfoI TCCNGGA 2 cut(s) 398, 931
PkrI GCNGC 5 cut(s) 239, 242, 245, 248, 258
PleI GAGTC 3 cut(s) 258, 730, 1092
PpsI GAGTC 3 cut(s) 258, 730, 1092
Psp6I CCWGG 3 cut(s) 398, 698, 1121
PspGI CCWGG 3 cut(s) 398, 698, 1121
PspN4I GGNNCC 3 cut(s) 21, 351, 930
PspPI GGNCC 3 cut(s) 20, 598, 841
PstI CTGCAG 1 cut(s) 289
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 1 cut(s) 928
RsaI GTAC 2 cut(s) 375, 1055
RsaNI GTAC 2 cut(s) 374, 1054
RseI CAYNNNNRTG 1 cut(s) 590
SaqAI TTAA 3 cut(s) 678, 729, 1002
SatI GCNGC 5 cut(s) 238, 241, 244, 247, 257
Sau3AI GATC 1 cut(s) 928
Sau96I GGNCC 3 cut(s) 20, 598, 841
SchI GAGTC 3 cut(s) 258, 731, 1093
ScrFI CCNGG 4 cut(s) 400, 700, 933, 1123
SfcI CTRYAG 1 cut(s) 285
SfuI TTCGAA 1 cut(s) 444
SinI GGWCC 3 cut(s) 20, 598, 841
SmiMI CAYNNNNRTG 1 cut(s) 590
SspI AATATT 1 cut(s) 683
SspMI CTAG 2 cut(s) 228, 1019
StyD4I CCNGG 4 cut(s) 398, 698, 931, 1121
TaaI ACNGT 3 cut(s) 217, 887, 1075
TaiI ACGT 2 cut(s) 379, 770
TaqI TCGA 2 cut(s) 444, 1038
Tru1I TTAA 3 cut(s) 678, 729, 1002
Tru9I TTAA 3 cut(s) 678, 729, 1002
TscAI CASTG 3 cut(s) 120, 220, 577
TseFI GTSAC 1 cut(s) 723
TseI GCWGC 5 cut(s) 237, 240, 243, 246, 256
Tsp45I GTSAC 1 cut(s) 723
TspDTI ATGAA 3 cut(s) 17, 295, 796
TspGWI ACGGA 1 cut(s) 597
TspRI CASTG 3 cut(s) 120, 220, 577
VpaK11BI GGWCC 3 cut(s) 20, 598, 841
XapI RAATTY 5 cut(s) 79, 302, 533, 731, 1139
XcmI CCANNNNNNNNNTGG 2 cut(s) 406, 750
XspI CTAG 2 cut(s) 228, 1019
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.