pycom03g15440
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014682
Physical Location & Seq
Forward (+)
16701413 .. 16702259
847 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 474 bp
ATGCGAGTTTATATCGGCTCATTCAATGACAAACCTGTAAATGAGGCTGCTACGGGTCCAGTCGGGAAAGAACTCTTTGAAAAGGAACAGGGAGATCTTTTGTCTGATTTAAACGATATTCCAAAGAAGGCTTGTGAGCGCAAAATCAATGAATTTGTGAAGCGTGCCAGAGCTGCCAAGATACATGCATGCATAATTAGCCATTTAAAGGCGGAGATGCCTGCCATGTTTTGGAAATCTACGGCTCAACAGAAGCTTATTGATAATTTGGAAGACGAATTTAAAAAGGTTCAGGGGGAGCACCATCTGCCTCCAGGGGATTTCCCGGACGTAAACCGCTACAGGGACATCTTGAGTGGTTACAGCTTTGACAAATTCGAGACGTTAAAGCCTAAGATGATAGATGCCGTCGATGAAATGCTGGGTTATGATATCCCGGAACTCTTGAAGAAATTCAGAAATCCATTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005886 GO:0005911 GO:0005929 GO:0006810 GO:0006886 GO:0006897 GO:0006950 GO:0006970 GO:0006972 GO:0006996 GO:0007275 GO:0007399 GO:0008104 GO:0008150 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009987 GO:0010008 GO:0010033 GO:0010830 GO:0010831 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0020016 GO:0020018 GO:0022008 GO:0022603 GO:0022607 GO:0030030 GO:0030031 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031175 GO:0031253 GO:0031410 GO:0031901 GO:0031982 GO:0032386 GO:0032388 GO:0032456 GO:0032501 GO:0032502 GO:0032879 GO:0033036 GO:0033365 GO:0034613 GO:0042221 GO:0042538 GO:0042886 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044441 GO:0044444 GO:0044446 GO:0044459 GO:0044463 GO:0044464 GO:0044782 GO:0045184 GO:0045595 GO:0045597 GO:0046907 GO:0048468 GO:0048518 GO:0048522 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051147 GO:0051149 GO:0051153 GO:0051155 GO:0051179 GO:0051234 GO:0051259 GO:0051260 GO:0051641 GO:0051649 GO:0051716 GO:0055037 GO:0055038 GO:0055044 GO:0060142 GO:0060143 GO:0060170 GO:0060271 GO:0060341 GO:0060627 GO:0061512 GO:0065003 GO:0065007 GO:0070727 GO:0070848 GO:0070887 GO:0070925 GO:0071310 GO:0071363 GO:0071495 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0097708 GO:0098588 GO:0098590 GO:0098657 GO:0098805 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:1901739 GO:1901741 GO:1990089 GO:1990090 GO:2001135 GO:2001137
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Dynamin_N EF-hand_4 EHD_N

Protein Analysis

158

Amino Acids

18.07

Weight (kDa)

6.11

Isoelectric Point (pI)

26.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF5600 PF18150 47 - 148 1.6e-38 Domain of unknown function (DUF5600)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 231
AciI CCGC 2 cut(s) 212, 337
AcsI RAATTY 4 cut(s) 152, 278, 374, 452
AfiI CCNNNNNNNGG 3 cut(s) 208, 231, 343
AgsI TTSAA 3 cut(s) 25, 80, 448
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 3 cut(s) 173, 256, 366
AluI AGCT 3 cut(s) 173, 256, 366
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 1 cut(s) 374
ApeKI GCWGC 2 cut(s) 47, 173
ApoI RAATTY 4 cut(s) 152, 278, 374, 452
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 56
AsuC2I CCSGG 2 cut(s) 326, 437
AvaII GGWCC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 34, 160
BccI CCATC 1 cut(s) 312
BceAI ACGGC 2 cut(s) 258, 392
BciT130I CCWGG 1 cut(s) 315
BcnI CCSGG 2 cut(s) 326, 437
BcoDI GTCTC 1 cut(s) 374
BfmI CTRYAG 1 cut(s) 340
BglII AGATCT 1 cut(s) 94
BisI GCNGC 2 cut(s) 48, 174
BlsI GCNGC 2 cut(s) 49, 175
Bme1390I CCNGG 3 cut(s) 315, 326, 437
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 57
BmrFI CCNGG 3 cut(s) 315, 326, 437
BmsI GCATC 2 cut(s) 207, 394
BpiI GAAGAC 1 cut(s) 279
BpmI CTGGAG 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 373
BpuMI CCSGG 2 cut(s) 326, 437
BsaJI CCNNGG 1 cut(s) 314
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 231, 343
Bse1I ACTGG 1 cut(s) 59
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 314
BseLI CCNNNNNNNGG 3 cut(s) 208, 231, 343
BseNI ACTGG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 34, 160
BseYI CCCAGC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 303
BsiSI CCGG 2 cut(s) 326, 437
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 3 cut(s) 208, 231, 343
BsmAI GTCTC 1 cut(s) 374
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 1 cut(s) 94
BspACI CCGC 2 cut(s) 212, 337
BspLI GGNNCC 1 cut(s) 57
BsrI ACTGG 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 1 cut(s) 94
Bst2UI CCWGG 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 307
BstC8I GCNNGC 3 cut(s) 165, 190, 222
BstDEI CTNAG 1 cut(s) 393
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 3 cut(s) 173, 198, 307
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 2 cut(s) 188, 192
BstSCI CCNGG 3 cut(s) 313, 324, 435
BstSFI CTRYAG 1 cut(s) 340
BstV1I GCAGC 2 cut(s) 34, 160
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
Cac8I GCNNGC 3 cut(s) 165, 190, 222
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 56
CviAII CATG 3 cut(s) 185, 189, 226
CviJI RGCY 9 cut(s) 18, 47, 131, 173, 201, 245, 256, 366, 391
CviKI_1 RGCY 9 cut(s) 18, 47, 131, 173, 201, 245, 256, 366, 391
DdeI CTNAG 1 cut(s) 393
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
DraI TTTAAA 3 cut(s) 111, 207, 283
EciI GGCGGA 1 cut(s) 227
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 313
EcoRV GATATC 1 cut(s) 433
EcoT22I ATGCAT 2 cut(s) 190, 194
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 3 cut(s) 188, 192, 229
FaiI YATR 6 cut(s) 12, 186, 190, 194, 227, 429
FaqI GGGAC 1 cut(s) 359
FatI CATG 3 cut(s) 184, 188, 225
Fnu4HI GCNGC 2 cut(s) 48, 174
Fsp4HI GCNGC 2 cut(s) 48, 174
GlaI GCGC 1 cut(s) 140
GluI GCNGC 2 cut(s) 48, 174
GsaI CCCAGC 1 cut(s) 425
GsuI CTGGAG 1 cut(s) 297
HapII CCGG 2 cut(s) 326, 437
HhaI GCGC 1 cut(s) 141
Hin1II CATG 3 cut(s) 188, 192, 229
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 254
HpaII CCGG 2 cut(s) 326, 437
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 2 cut(s) 106, 458
Hpy188III TCNNGA 4 cut(s) 64, 352, 379, 445
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 1 cut(s) 413
HpyAV CCTTC 1 cut(s) 121
HpyCH4IV ACGT 2 cut(s) 330, 383
HpyCH4V TGCA 2 cut(s) 188, 192
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 198, 307
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 2 cut(s) 330, 383
Hsp92II CATG 3 cut(s) 188, 192, 229
HspAI GCGC 1 cut(s) 139
Kzo9I GATC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 298
Lsp1109I GCAGC 2 cut(s) 34, 160
LweI GCATC 2 cut(s) 207, 394
MaeII ACGT 2 cut(s) 330, 383
MaeIII GTNAC 1 cut(s) 359
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MboII GAAGA 2 cut(s) 284, 460
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 6 cut(s) 152, 195, 265, 278, 374, 452
MnlI CCTC 2 cut(s) 37, 321
Mph1103I ATGCAT 2 cut(s) 190, 194
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 4 cut(s) 110, 206, 282, 386
MspI CCGG 2 cut(s) 326, 437
MspR9I CCNGG 3 cut(s) 315, 326, 437
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 3 cut(s) 173, 198, 307
NciI CCSGG 2 cut(s) 326, 437
NdeII GATC 1 cut(s) 94
NlaIII CATG 3 cut(s) 188, 192, 229
NlaIV GGNNCC 1 cut(s) 57
NsiI ATGCAT 2 cut(s) 190, 194
NspI RCATGY 2 cut(s) 188, 192
PaeI GCATGC 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 231
PfoI TCCNGGA 2 cut(s) 324, 435
PkrI GCNGC 2 cut(s) 49, 175
Psp6I CCWGG 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 421
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 57
PspPI GGNCC 1 cut(s) 56
PsuI RGATCY 1 cut(s) 94
SaqAI TTAA 4 cut(s) 110, 206, 282, 386
SatI GCNGC 2 cut(s) 48, 174
Sau3AI GATC 1 cut(s) 94
Sau96I GGNCC 1 cut(s) 56
ScrFI CCNGG 3 cut(s) 315, 326, 437
SduI GDGCHC 1 cut(s) 303
SetI ASST 7 cut(s) 37, 175, 258, 291, 333, 368, 386
SfaNI GCATC 2 cut(s) 207, 394
SfcI CTRYAG 1 cut(s) 340
SinI GGWCC 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 352
SmoI CTYRAG 1 cut(s) 352
SphI GCATGC 1 cut(s) 192
Sse9I AATT 6 cut(s) 152, 195, 265, 278, 374, 452
SsiI CCGC 2 cut(s) 212, 337
StyD4I CCNGG 3 cut(s) 313, 324, 435
TaiI ACGT 2 cut(s) 333, 386
TaqI TCGA 3 cut(s) 378, 411, 468
TasI AATT 6 cut(s) 152, 195, 265, 278, 374, 452
Tru1I TTAA 4 cut(s) 110, 206, 282, 386
Tru9I TTAA 4 cut(s) 110, 206, 282, 386
TseI GCWGC 2 cut(s) 47, 173
TspDTI ATGAA 2 cut(s) 165, 429
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 4 cut(s) 152, 278, 374, 452
XceI RCATGY 2 cut(s) 188, 192
XmnI GAANNNNTTC 1 cut(s) 452
Zsp2I ATGCAT 2 cut(s) 190, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.