pycom03g15520

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007352
Physical Location & Seq
Reverse (-)
16768081 .. 16770052
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
ATGAGGAGGAGACCTGGAATCGGCGGCTTGCAGAACGCCGCCGCCTCTAGGGACCAATATCGTCTGCTGGGCGAGAATGTTGCCAAGATCAGAACCGATCTCATGAAGGAGCAGCTCACCACTTTCCGAACGCAGTTGGAAGATTTTGCTCGGAAGCACAAGAATGACATTCGAAAGAACCCAGCTTTCAGATCACAATTTCACAACATGTGCACGAAGGTCGGAGTAGATCCCTTGGCATCCAATAAGGGTTTCTGGGCAGAGCTTTTGGGGATTGGTGATTTCTACTATGAACTTGGGGTGCAAATTGTTGACATTTGCTTGGCAACAAGACCCCACAATGGGGGTTTGATCAACCTGCAGGAACTCTGCAGTCTGCTTCGTCAGAGGCGGAAGAGCGACCGTGAAGCTGTTTCTGAGGATGATTGCCTGCGTGCTATAAGCAAGCTCAAGGTATTAGGTAATGGTTATGAGGTTATTTCTGTTGGAAAGAGAAAGCTTGTTCGTTCTGTTCCAACTGAGTTGAACAAAGACCATAACGAAATTTTAGAGCTAGCCCAGGCTCAAGGCTTTGTGACTGTCCCTGAGGTGGAGAGACGGCTATCTTGGACTACTGGTCGTGCAATCGATGCCTTTGACACTTTATTAGATGAGGGTCTTGCAATGATTGATGATGGTCACAAAGATGGTGTACGCCGATATTGGTTCCCCTGTGTATCTTCCATCTCCGCTCCACTAGGAGCCGACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

251

Amino Acids

28.13

Weight (kDa)

8.79

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAP30 PF04157 6 - 225 7.9e-55 EAP30/Vps36 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 366
AccBSI CCGCTC 1 cut(s) 731
AciI CCGC 5 cut(s) 24, 39, 42, 391, 729
AclWI GGATC 1 cut(s) 224
AcsI RAATTY 1 cut(s) 543
AfaI GTAC 1 cut(s) 693
AfiI CCNNNNNNNGG 6 cut(s) 20, 48, 341, 342, 343, 589
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 1 cut(s) 526
AhdI GACNNNNNGTC 1 cut(s) 615
AjnI CCWGG 2 cut(s) 13, 558
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 7 cut(s) 115, 185, 265, 410, 448, 499, 553
AluI AGCT 7 cut(s) 115, 185, 265, 410, 448, 499, 553
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 2 cut(s) 4, 589
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 1 cut(s) 224
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 1 cut(s) 112
ApoI RAATTY 1 cut(s) 543
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 109, 290
AsuII TTCGAA 1 cut(s) 172
AsuNHI GCTAGC 1 cut(s) 553
AvaII GGWCC 1 cut(s) 52
AxyI CCTNAGG 1 cut(s) 585
BaeGI GKGCMC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 124
BccI CCATC 3 cut(s) 668, 680, 731
BceAI ACGGC 1 cut(s) 614
BcgI CGANNNNNNTGC 4 cut(s) 62, 96, 202, 236
BciT130I CCWGG 2 cut(s) 15, 560
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 4, 589
BfaI CTAG 3 cut(s) 48, 554, 737
BfmI CTRYAG 2 cut(s) 359, 370
BfuAI ACCTGC 1 cut(s) 366
BisI GCNGC 4 cut(s) 25, 39, 42, 113
BlsI GCNGC 4 cut(s) 26, 40, 43, 114
Bme1390I CCNGG 2 cut(s) 15, 560
Bme18I GGWCC 1 cut(s) 52
BmeRI GACNNNNNGTC 1 cut(s) 615
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 3 cut(s) 53, 707, 742
BmrFI CCNGG 2 cut(s) 15, 560
BmsI GCATC 2 cut(s) 248, 619
BmtI GCTAGC 1 cut(s) 557
Bpu14I TTCGAA 1 cut(s) 172
BpuEI CTTGAG 2 cut(s) 434, 549
Bsa29I ATCGAT 1 cut(s) 627
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 2 cut(s) 234, 558
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 48, 341, 342, 343, 589
Bse1I ACTGG 1 cut(s) 619
Bse21I CCTNAGG 1 cut(s) 585
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 2 cut(s) 15, 560
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 2 cut(s) 234, 558
BseGI GGATG 2 cut(s) 239, 427
BseLI CCNNNNNNNGG 6 cut(s) 20, 48, 341, 342, 343, 589
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 3 cut(s) 408, 510, 576
BseNI ACTGG 1 cut(s) 619
BseRI GAGGAG 2 cut(s) 19, 22
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 1 cut(s) 124
BseYI CCCAGC 2 cut(s) 67, 181
Bsh1285I CGRYCG 1 cut(s) 403
BshVI ATCGAT 1 cut(s) 627
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 215
BslFI GGGAC 2 cut(s) 65, 566
BslI CCNNNNNNNGG 6 cut(s) 20, 48, 341, 342, 343, 589
BsmAI GTCTC 2 cut(s) 4, 589
BsmBI CGTCTC 1 cut(s) 589
BsmFI GGGAC 2 cut(s) 65, 566
Bso31I GGTCTC 1 cut(s) 4
Bsp119I TTCGAA 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 5 cut(s) 87, 97, 191, 229, 351
BspACI CCGC 5 cut(s) 24, 39, 42, 391, 729
BspCNI CTCAG 3 cut(s) 409, 511, 577
BspDI ATCGAT 1 cut(s) 627
BspHI TCATGA 1 cut(s) 102
BspLI GGNNCC 3 cut(s) 53, 707, 742
BspMAI CTGCAG 2 cut(s) 363, 374
BspMI ACCTGC 1 cut(s) 366
BspOI GCTAGC 1 cut(s) 557
BspPI GGATC 1 cut(s) 224
BspQI GCTCTTC 1 cut(s) 389
BspT104I TTCGAA 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 4
BsrBI CCGCTC 1 cut(s) 731
BsrDI GCAATG 1 cut(s) 669
BsrI ACTGG 1 cut(s) 619
BssECI CCNNGG 2 cut(s) 234, 558
BssMI GATC 5 cut(s) 87, 97, 191, 229, 351
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 2 cut(s) 15, 560
Bst4CI ACNGT 3 cut(s) 404, 580, 749
Bst6I CTCTTC 1 cut(s) 389
BstAPI GCANNNNNTGC 1 cut(s) 629
BstBI TTCGAA 1 cut(s) 172
BstC8I GCNNGC 5 cut(s) 29, 431, 435, 446, 555
BstDEI CTNAG 3 cut(s) 417, 519, 585
BstF5I GGATG 2 cut(s) 239, 427
BstKTI GATC 5 cut(s) 90, 100, 194, 232, 354
BstMAI GTCTC 2 cut(s) 4, 589
BstMBI GATC 5 cut(s) 87, 97, 191, 229, 351
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 1 cut(s) 629
BstNI CCWGG 2 cut(s) 15, 560
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 2 cut(s) 13, 558
BstSFI CTRYAG 2 cut(s) 359, 370
BstSLI GKGCMC 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
Bsu15I ATCGAT 1 cut(s) 627
Bsu36I CCTNAGG 1 cut(s) 585
BsuTUI ATCGAT 1 cut(s) 627
BtsCI GGATG 2 cut(s) 239, 427
BveI ACCTGC 1 cut(s) 366
Cac8I GCNNGC 5 cut(s) 29, 431, 435, 446, 555
CciI TCATGA 1 cut(s) 102
Cfr13I GGNCC 1 cut(s) 52
ClaI ATCGAT 1 cut(s) 627
Csp6I GTAC 1 cut(s) 692
CviAII CATG 2 cut(s) 103, 208
CviQI GTAC 1 cut(s) 692
DdeI CTNAG 3 cut(s) 417, 519, 585
DpnI GATC 5 cut(s) 89, 99, 193, 231, 353
DpnII GATC 5 cut(s) 87, 97, 191, 229, 351
DriI GACNNNNNGTC 1 cut(s) 615
Eam1104I CTCTTC 1 cut(s) 389
Eam1105I GACNNNNNGTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 389
EciI GGCGGA 1 cut(s) 406
Eco130I CCWWGG 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 1 cut(s) 52
Eco81I CCTNAGG 1 cut(s) 585
EcoRII CCWGG 2 cut(s) 13, 558
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 2 cut(s) 106, 211
FaiI YATR 6 cut(s) 104, 209, 291, 440, 471, 537
FaqI GGGAC 2 cut(s) 65, 566
FatI CATG 2 cut(s) 102, 207
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 4 cut(s) 25, 39, 42, 113
FokI GGATG 2 cut(s) 226, 434
Fsp4HI GCNGC 4 cut(s) 25, 39, 42, 113
FspBI CTAG 3 cut(s) 48, 554, 737
GluI GCNGC 4 cut(s) 25, 39, 42, 113
GsaI CCCAGC 2 cut(s) 71, 185
Hin1II CATG 2 cut(s) 106, 211
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 18
HphI GGTGA 2 cut(s) 109, 290
Hpy166II GTNNAC 3 cut(s) 213, 313, 692
Hpy188I TCNGA 7 cut(s) 92, 128, 153, 191, 224, 387, 418
Hpy188III TCNNGA 1 cut(s) 103
Hpy8I GTNNAC 3 cut(s) 213, 313, 692
HpyAV CCTTC 2 cut(s) 100, 211
HpyCH4III ACNGT 3 cut(s) 404, 580, 749
HpyCH4V TGCA 7 cut(s) 31, 213, 304, 361, 372, 623, 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 629
HpyF3I CTNAG 3 cut(s) 417, 519, 585
Hsp92II CATG 2 cut(s) 106, 211
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 5 cut(s) 87, 97, 191, 229, 351
LguI GCTCTTC 1 cut(s) 389
LmnI GCTCC 3 cut(s) 109, 736, 740
Lsp1109I GCAGC 1 cut(s) 124
LweI GCATC 2 cut(s) 248, 619
MaeI CTAG 3 cut(s) 48, 554, 737
MaeIII GTNAC 2 cut(s) 574, 677
MalI GATC 5 cut(s) 89, 99, 193, 231, 353
MbiI CCGCTC 1 cut(s) 731
MboI GATC 5 cut(s) 87, 97, 191, 229, 351
MboII GAAGA 3 cut(s) 152, 406, 711
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 3 cut(s) 197, 306, 543
MmeI TCCRAC 4 cut(s) 117, 202, 466, 539
MnlI CCTC 6 cut(s) 55, 381, 412, 466, 580, 646
MseI TTAA 1 cut(s) 751
MslI CAYNNNNRTG 2 cut(s) 162, 684
MspR9I CCNGG 2 cut(s) 15, 560
MvaI CCWGG 2 cut(s) 15, 560
MwoI GCNNNNNNNGC 1 cut(s) 629
NdeII GATC 5 cut(s) 87, 97, 191, 229, 351
NheI GCTAGC 1 cut(s) 553
NlaIII CATG 2 cut(s) 106, 211
NlaIV GGNNCC 3 cut(s) 53, 707, 742
NmuCI GTSAC 2 cut(s) 574, 677
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 1 cut(s) 172
PagI TCATGA 1 cut(s) 102
PciI ACATGT 1 cut(s) 207
PciSI GCTCTTC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 18
PkrI GCNGC 4 cut(s) 26, 40, 43, 114
PscI ACATGT 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 13, 558
PspFI CCCAGC 2 cut(s) 67, 181
PspGI CCWGG 2 cut(s) 13, 558
PspN4I GGNNCC 3 cut(s) 53, 707, 742
PspPI GGNCC 1 cut(s) 52
PstI CTGCAG 2 cut(s) 363, 374
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 1 cut(s) 693
RsaNI GTAC 1 cut(s) 692
RseI CAYNNNNRTG 2 cut(s) 162, 684
SapI GCTCTTC 1 cut(s) 389
SaqAI TTAA 1 cut(s) 751
SatI GCNGC 4 cut(s) 25, 39, 42, 113
Sau3AI GATC 5 cut(s) 87, 97, 191, 229, 351
Sau96I GGNCC 1 cut(s) 52
SbfI CCTGCAGG 1 cut(s) 363
ScrFI CCNGG 2 cut(s) 15, 560
SdaI CCTGCAGG 1 cut(s) 363
SduI GDGCHC 1 cut(s) 215
SfaNI GCATC 2 cut(s) 248, 619
SfcI CTRYAG 2 cut(s) 359, 370
SfuI TTCGAA 1 cut(s) 172
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 2 cut(s) 162, 684
SmlI CTYRAG 2 cut(s) 449, 564
SmoI CTYRAG 2 cut(s) 449, 564
Sse8387I CCTGCAGG 1 cut(s) 363
Sse9I AATT 3 cut(s) 197, 306, 543
SsiI CCGC 5 cut(s) 24, 39, 42, 391, 729
SspMI CTAG 3 cut(s) 48, 554, 737
StyD4I CCNGG 2 cut(s) 13, 558
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 3 cut(s) 404, 580, 749
TaqI TCGA 2 cut(s) 172, 627
TasI AATT 3 cut(s) 197, 306, 543
TauI GCSGC 3 cut(s) 27, 41, 44
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 1 cut(s) 751
Tru9I TTAA 1 cut(s) 751
TseFI GTSAC 2 cut(s) 574, 677
TseI GCWGC 1 cut(s) 112
Tsp45I GTSAC 2 cut(s) 574, 677
TspDTI ATGAA 2 cut(s) 119, 306
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 1 cut(s) 543
XceI RCATGY 1 cut(s) 211
XspI CTAG 3 cut(s) 48, 554, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.