pycom03g23710

ATPase WRNIP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009307
Physical Location & Seq
Reverse (-)
23544478 .. 23546250
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23710.1

Sequence Viewer

Length: 1773 bp
ATGGAAATGGAGCAGCTGCTGAGCATGGGCTTCCCCAGCGAGTTGGCGGCCCAAGCCCTAGCTGCCACAGGAACAGGGGGCAACTCCATTCTCAAGGCCACAGAATGGATTCTCAACCGCGAATCATCCACATCCGCCGGCACTAGCACGAATCCGAGCCCGATTTCATCCGGGCGGCCTTGCCAACCCAAGCTCGACCGCTTCTTTCTGTTCCAGTCCAAGCCTCCTTCCTCCGATTCTCCACGTCCCGTCCCCGCTTCCGCTCGCAAACTACAAGAGGAATCGGAATCCACCCCAGCCGCAGGGGGAGAGGGGCCAGAAGAAGAAGCTCCACTCTCCAAGTCGAAACGTCTCAAACTAGCGATCCCAGCAAACCCCAAAAATGTCGACAACAACACCAACGAGCCGTTGTCGGAGCGCATGCGCCCTCGCACCTTAGACGACGTCGTGGGCCAAGACCACCTACTTGCCCCAAACTCTCTGCTCCGCTCCGCAATCGACTGCGAACGTCTCCCTTCTCTTGTGTTCTGGGGTCCACCAGGCGTCGGCAAGACCTCCATTGCCAAATCCATCATCAATTCCGCCTCCCGCCCTTGCTCCTCCTACCGATTCGTTTCGTTGTCCGCCGTCACTTGCGGGGTTAAAGACGTGAGGGACGCTATCGACGAGGCCCGCAAGAAGAAACAGGCGGCTAAGAAAAAACAAACAGTGCTGTTCGTGGACGAGGTTCACAGGTTCAACAAGTCACAGCAGGACTCGTTCTTGCCCGTCATCGAAGACGGCAGTATAGTCTTCATGGGGGCCACCACCGAGAACCCTTCCTTCCATTTGATCAGGCCGCTCTTGTCTCGCTGCCGAGTTCTTGTTCTCCACCCTCTGAGACCCCACGCGGTTGAGCTCCTTCTCAAGCGGGCCGCCAATGACTCCGGTAAGGGATTGGCTCCCGGTGTGCGGATGCATGTACATGTCAATGATGAGGCCATCCATTTCATCTCCGCCAACTGCGATGGCGATGCTCGGGTGGCACTCAATGCCTTGGAAGTTTCTGCAACAACAGCAGCTGCCCGAGCAGCCTCTACCTCTAGTTCCTCGATCTCTAACTCTACCCCTACCTCTACGAATGATGATGATGATGATGATCATAATAAGTTAGTGGCTACTGCTGTTGTTACCTTAGAGGATGCCAAAGAGGCCTTGCAATGCAAACATGTCGCCTCTGACAAAGCGGGGGAAGAACACTATAATCTCATCAGTGCCCTTCACAAGTCCATGAGAGGAAGTGATGCTAATGCGGCCATTTATTGGTTGGCAAGGATGCTAGAAGGAGGCCAAGAACCTCTATATATTGCCCGCAGACTCGTACGGTTTGCAAGTGAAGATGTTGGGTTGGCTGACCCATCCGCACTGAGCCAGGCCGTTGCTTGTCATCAGGCTTGCCATTTTCTGGGAATGCCAGAGTGCAATGTCATTCTTGCACAGTGTGTGGCTTACTTGGCATTGGCTCCTAAATCTGTCGCTGTTTATAAAGCAATAGGGAGTGCACAACAAGTGGTGAGGGACTCTGTCGGACAGAATGAGGGAGTGCCTCTTCATCTGAGGAATGCGCCTACCAAGCTAATGAAGGAACTTGGGTATGCCAACGGATACATCTACCCACCTGACAATCCCTCAGCCAGTGCCACGCAGGCATATCTACCACCCTCACTTGAAGGTTACAAGTTCCTGGATTGGCCGATCACTAGTAATAGCACCAAAACCAAATCCGATGGATGA

Protein Analysis

591

Amino Acids

63.36

Weight (kDa)

6.74

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_7 PF22562 3 - 40 1.8e-07 UBA-like domain
RuvB_N PF05496 141 - 264 4.4e-09 Holliday junction DNA helicase RuvB P-loop domain
AAA_5 PF07728 174 - 270 8e-06 AAA domain (dynein-related subfamily)
AAA PF00004 175 - 290 1e-16 ATPase family associated with various cellular activities (AAA)
AAA_assoc_2 PF16193 320 - 425 3e-21 AAA C-terminal domain
MgsA_C PF12002 426 - 576 7.6e-59 MgsA AAA+ ATPase C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1524
AatII GACGTC 1 cut(s) 447
AccB7I CCANNNNNTGG 3 cut(s) 105, 1302, 1444
AccBSI CCGCTC 3 cut(s) 263, 489, 841
AccI GTMKAC 1 cut(s) 387
AccII CGCG 2 cut(s) 120, 890
AclWI GGATC 1 cut(s) 358
AcoI YGGCCR 2 cut(s) 1293, 1730
AcyI GRCGYC 2 cut(s) 444, 543
AdeI CACNNNGTG 1 cut(s) 1481
AfaI GTAC 2 cut(s) 963, 1362
AfiI CCNNNNNNNGG 6 cut(s) 105, 302, 545, 951, 1302, 1444
AflIII ACRYGT 2 cut(s) 964, 1207
AgsI TTSAA 2 cut(s) 739, 1709
AhlI ACTAGT 1 cut(s) 1739
AjiI CACGTC 2 cut(s) 245, 649
AjnI CCWGG 3 cut(s) 538, 1410, 1722
AluBI AGCT 7 cut(s) 16, 62, 193, 329, 898, 1061, 1615
AluI AGCT 7 cut(s) 16, 62, 193, 329, 898, 1061, 1615
Alw21I GWGCWC 2 cut(s) 900, 1543
Alw26I GTCTC 4 cut(s) 356, 515, 852, 874
Alw44I GTGCAC 1 cut(s) 1539
AlwI GGATC 1 cut(s) 358
AlwNI CAGNNNCTG 2 cut(s) 19, 1061
Ama87I CYCGRG 2 cut(s) 1017, 1065
ApaLI GTGCAC 1 cut(s) 1539
ApeKI GCWGC 7 cut(s) 13, 16, 62, 852, 1058, 1061, 1070
Asp700I GAANNNNTTC 1 cut(s) 108
AspLEI GCGC 3 cut(s) 420, 426, 1606
AspS9I GGNCC 7 cut(s) 49, 314, 451, 533, 670, 801, 912
AsuC2I CCSGG 2 cut(s) 172, 945
AsuHPI GGTGA 1 cut(s) 1564
AvaI CYCGRG 2 cut(s) 1017, 1065
AvaII GGWCC 1 cut(s) 533
BaeGI GKGCMC 2 cut(s) 1258, 1543
BanII GRGCYC 2 cut(s) 161, 900
BbsI GAAGAC 2 cut(s) 783, 784
Bbv12I GWGCWC 2 cut(s) 900, 1543
BbvCI CCTCAGC 1 cut(s) 1669
BbvI GCAGC 7 cut(s) 3, 25, 49, 839, 1048, 1070, 1082
BccI CCATC 5 cut(s) 578, 989, 1001, 1405, 1760
BceAI ACGGC 4 cut(s) 391, 611, 796, 1400
BcgI CGANNNNNNTGC 4 cut(s) 995, 1029, 1192, 1226
BciT130I CCWGG 3 cut(s) 540, 1412, 1724
BciVI GTATCC 1 cut(s) 1637
BclI TGATCA 2 cut(s) 831, 1138
BcnI CCSGG 2 cut(s) 172, 945
BcoDI GTCTC 4 cut(s) 356, 515, 852, 874
BcuI ACTAGT 1 cut(s) 1739
BfaI CTAG 6 cut(s) 59, 144, 359, 1083, 1319, 1740
BfuI GTATCC 1 cut(s) 1637
BglI GCCNNNNNGGC 2 cut(s) 1190, 1685
BlpI GCTNAGC 1 cut(s) 20
Bme1390I CCNGG 5 cut(s) 172, 540, 945, 1412, 1724
Bme18I GGWCC 1 cut(s) 533
BmeT110I CYCGRG 2 cut(s) 1017, 1065
BmgBI CACGTC 2 cut(s) 245, 649
BmgT120I GGNCC 7 cut(s) 49, 314, 451, 533, 670, 801, 912
BmiI GGNNCC 5 cut(s) 315, 534, 802, 942, 1503
BmrFI CCNGG 5 cut(s) 172, 540, 945, 1412, 1724
BmsI GCATC 5 cut(s) 945, 1003, 1171, 1273, 1305
BpiI GAAGAC 2 cut(s) 783, 784
Bpu10I CCTNAGC 1 cut(s) 1669
Bpu1102I GCTNAGC 1 cut(s) 20
BpuEI CTTGAG 2 cut(s) 77, 890
BpuMI CCSGG 2 cut(s) 172, 945
BsaBI GATNNNNATC 1 cut(s) 1137
BsaHI GRCGYC 2 cut(s) 444, 543
BsaI GGTCTC 1 cut(s) 874
BsaJI CCNNGG 1 cut(s) 1035
BsaWI WCCGGW 1 cut(s) 926
Bsc4I CCNNNNNNNGG 6 cut(s) 105, 302, 545, 951, 1302, 1444
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 2 cut(s) 214, 1674
Bse3DI GCAATG 3 cut(s) 558, 1205, 1468
Bse8I GATNNNNATC 1 cut(s) 1137
BseBI CCWGG 3 cut(s) 540, 1412, 1724
BseDI CCNNGG 1 cut(s) 1035
BseGI GGATG 8 cut(s) 125, 131, 167, 960, 981, 1186, 1320, 1397
BseJI GATNNNNATC 1 cut(s) 1137
BseLI CCNNNNNNNGG 6 cut(s) 105, 302, 545, 951, 1302, 1444
BseMI GCAATG 3 cut(s) 558, 1205, 1468
BseMII CTCAG 5 cut(s) 11, 869, 1397, 1586, 1683
BseNI ACTGG 2 cut(s) 214, 1674
BseRI GAGGAG 1 cut(s) 589
BseSI GKGCMC 2 cut(s) 1258, 1543
BseXI GCAGC 7 cut(s) 3, 25, 49, 839, 1048, 1070, 1082
BseYI CCCAGC 3 cut(s) 35, 295, 367
Bsh1236I CGCG 2 cut(s) 120, 890
Bsh1285I CGRYCG 1 cut(s) 199
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 2 cut(s) 900, 1543
BsiHKCI CYCGRG 2 cut(s) 1017, 1065
BsiSI CCGG 4 cut(s) 138, 171, 927, 945
BsiWI CGTACG 1 cut(s) 1360
BslFI GGGAC 4 cut(s) 231, 236, 668, 1571
BslI CCNNNNNNNGG 6 cut(s) 105, 302, 545, 951, 1302, 1444
BsmAI GTCTC 4 cut(s) 356, 515, 852, 874
BsmBI CGTCTC 2 cut(s) 356, 515
BsmFI GGGAC 4 cut(s) 231, 236, 668, 1571
BsmI GAATGC 2 cut(s) 1455, 1606
Bso31I GGTCTC 1 cut(s) 874
BsoBI CYCGRG 2 cut(s) 1017, 1065
Bsp1286I GDGCHC 4 cut(s) 161, 900, 1258, 1543
Bsp1407I TGTACA 1 cut(s) 961
Bsp143I GATC 5 cut(s) 363, 831, 1092, 1138, 1734
Bsp1720I GCTNAGC 1 cut(s) 20
BspCNI CTCAG 5 cut(s) 12, 870, 1398, 1587, 1682
BspFNI CGCG 2 cut(s) 120, 890
BspLI GGNNCC 5 cut(s) 315, 534, 802, 942, 1503
BspPI GGATC 1 cut(s) 358
BspTNI GGTCTC 1 cut(s) 874
BsrBI CCGCTC 3 cut(s) 263, 489, 841
BsrDI GCAATG 3 cut(s) 558, 1205, 1468
BsrFI RCCGGY 1 cut(s) 137
BsrGI TGTACA 1 cut(s) 961
BsrI ACTGG 2 cut(s) 214, 1674
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 1035
BssMI GATC 5 cut(s) 363, 831, 1092, 1138, 1734
BssNI GRCGYC 2 cut(s) 444, 543
BssT1I CCWWGG 1 cut(s) 1035
Bst2UI CCWGG 3 cut(s) 540, 1412, 1724
Bst4CI ACNGT 3 cut(s) 709, 1365, 1479
Bst6I CTCTTC 1 cut(s) 1593
BstACI GRCGYC 2 cut(s) 444, 543
BstAPI GCANNNNNTGC 1 cut(s) 1031
BstAUI TGTACA 1 cut(s) 961
BstC8I GCNNGC 8 cut(s) 139, 265, 422, 673, 912, 1351, 1435, 1686
BstDEI CTNAG 8 cut(s) 20, 436, 693, 878, 1174, 1406, 1595, 1669
BstF5I GGATG 8 cut(s) 125, 131, 167, 960, 981, 1186, 1320, 1397
BstFNI CGCG 2 cut(s) 120, 890
BstHHI GCGC 3 cut(s) 420, 426, 1606
BstKTI GATC 5 cut(s) 366, 834, 1095, 1141, 1737
BstMAI GTCTC 4 cut(s) 356, 515, 852, 874
BstMBI GATC 5 cut(s) 363, 831, 1092, 1138, 1734
BstMCI CGRYCG 1 cut(s) 199
BstNI CCWGG 3 cut(s) 540, 1412, 1724
BstNSI RCATGY 4 cut(s) 424, 962, 968, 1211
BstSCI CCNGG 5 cut(s) 170, 538, 943, 1410, 1722
BstSLI GKGCMC 2 cut(s) 1258, 1543
BstUI CGCG 2 cut(s) 120, 890
BstV1I GCAGC 7 cut(s) 3, 25, 49, 839, 1048, 1070, 1082
BstV2I GAAGAC 2 cut(s) 783, 784
BstXI CCANNNNNNTGG 1 cut(s) 43
BsuI GTATCC 1 cut(s) 1637
BtgZI GCGATG 2 cut(s) 1020, 1026
BtrI CACGTC 2 cut(s) 245, 649
BtsCI GGATG 8 cut(s) 125, 131, 167, 960, 981, 1186, 1320, 1397
BtsIMutI CAGTG 5 cut(s) 714, 1258, 1403, 1484, 1681
Cac8I GCNNGC 8 cut(s) 139, 265, 422, 673, 912, 1351, 1435, 1686
CaiI CAGNNNCTG 2 cut(s) 19, 1061
CfoI GCGC 3 cut(s) 420, 426, 1606
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 7 cut(s) 49, 314, 451, 533, 670, 801, 912
CseI GACGC 2 cut(s) 532, 665
Csp6I GTAC 2 cut(s) 962, 1361
CviAII CATG 7 cut(s) 25, 421, 796, 959, 965, 1208, 1270
CviQI GTAC 2 cut(s) 962, 1361
DdeI CTNAG 8 cut(s) 20, 436, 693, 878, 1174, 1406, 1595, 1669
DpnI GATC 5 cut(s) 365, 833, 1094, 1140, 1736
DpnII GATC 5 cut(s) 363, 831, 1092, 1138, 1734
DraIII CACNNNGTG 1 cut(s) 1481
EaeI YGGCCR 2 cut(s) 1293, 1730
Eam1104I CTCTTC 1 cut(s) 1593
EarI CTCTTC 1 cut(s) 1593
EciI GGCGGA 4 cut(s) 124, 571, 613, 985
Ecl136II GAGCTC 1 cut(s) 898
Eco130I CCWWGG 1 cut(s) 1035
Eco147I AGGCCT 1 cut(s) 1193
Eco24I GRGCYC 2 cut(s) 161, 900
Eco31I GGTCTC 1 cut(s) 874
Eco47I GGWCC 1 cut(s) 533
Eco53kI GAGCTC 1 cut(s) 898
Eco88I CYCGRG 2 cut(s) 1017, 1065
EcoICRI GAGCTC 1 cut(s) 898
EcoRII CCWGG 3 cut(s) 538, 1410, 1722
EcoT14I CCWWGG 1 cut(s) 1035
EcoT22I ATGCAT 1 cut(s) 960
EcoT38I GRGCYC 2 cut(s) 161, 900
ErhI CCWWGG 1 cut(s) 1035
Esp3I CGTCTC 2 cut(s) 356, 515
FaeI CATG 7 cut(s) 28, 424, 799, 962, 968, 1211, 1273
FaqI GGGAC 4 cut(s) 231, 236, 668, 1571
FatI CATG 7 cut(s) 24, 420, 795, 958, 964, 1207, 1269
FauI CCCGC 7 cut(s) 262, 596, 629, 680, 903, 1219, 1358
FbaI TGATCA 2 cut(s) 831, 1138
FblI GTMKAC 1 cut(s) 387
FokI GGATG 8 cut(s) 112, 118, 154, 967, 968, 1193, 1327, 1384
FriOI GRGCYC 2 cut(s) 161, 900
FspBI CTAG 6 cut(s) 59, 144, 359, 1083, 1319, 1740
GlaI GCGC 3 cut(s) 419, 425, 1605
GsaI CCCAGC 3 cut(s) 39, 299, 371
HapII CCGG 4 cut(s) 138, 171, 927, 945
HgaI GACGC 2 cut(s) 532, 665
HhaI GCGC 3 cut(s) 420, 426, 1606
Hin1I GRCGYC 2 cut(s) 444, 543
Hin1II CATG 7 cut(s) 28, 424, 799, 962, 968, 1211, 1273
Hin6I GCGC 3 cut(s) 418, 424, 1604
HinP1I GCGC 3 cut(s) 418, 424, 1604
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HpaII CCGG 4 cut(s) 138, 171, 927, 945
HphI GGTGA 1 cut(s) 1564
Hpy166II GTNNAC 5 cut(s) 388, 536, 721, 730, 1541
Hpy188I TCNGA 9 cut(s) 156, 235, 286, 415, 879, 1219, 1568, 1596, 1765
Hpy8I GTNNAC 5 cut(s) 388, 536, 721, 730, 1541
Hpy99I CGWCG 4 cut(s) 446, 449, 548, 668
HpyAV CCTTC 9 cut(s) 237, 525, 828, 832, 911, 1268, 1316, 1615, 1703
HpyCH4III ACNGT 3 cut(s) 709, 1365, 1479
HpyCH4IV ACGT 5 cut(s) 244, 349, 444, 508, 648
HpyCH4V TGCA 8 cut(s) 958, 1049, 1198, 1203, 1370, 1461, 1475, 1541
HpyF3I CTNAG 8 cut(s) 20, 436, 693, 878, 1174, 1406, 1595, 1669
HpySE526I ACGT 5 cut(s) 244, 349, 444, 508, 648
Hsp92I GRCGYC 2 cut(s) 444, 543
Hsp92II CATG 7 cut(s) 28, 424, 799, 962, 968, 1211, 1273
HspAI GCGC 3 cut(s) 418, 424, 1604
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Ksp22I TGATCA 2 cut(s) 831, 1138
Kzo9I GATC 5 cut(s) 363, 831, 1092, 1138, 1734
LmnI GCTCC 9 cut(s) 10, 334, 415, 489, 494, 602, 903, 946, 1507
Lsp1109I GCAGC 7 cut(s) 3, 25, 49, 839, 1048, 1070, 1082
LweI GCATC 5 cut(s) 945, 1003, 1171, 1273, 1305
MaeI CTAG 6 cut(s) 59, 144, 359, 1083, 1319, 1740
MaeII ACGT 5 cut(s) 244, 349, 444, 508, 648
MaeIII GTNAC 4 cut(s) 628, 744, 1168, 1712
MalI GATC 5 cut(s) 365, 833, 1094, 1140, 1736
MbiI CCGCTC 3 cut(s) 263, 489, 841
MboI GATC 5 cut(s) 363, 831, 1092, 1138, 1734
MboII GAAGA 8 cut(s) 332, 335, 691, 784, 788, 1244, 1388, 1580
MhlI GDGCHC 4 cut(s) 161, 900, 1258, 1543
MluCI AATT 1 cut(s) 577
MlyI GAGTC 4 cut(s) 749, 917, 1350, 1553
MmeI TCCRAC 2 cut(s) 393, 1546
Mph1103I ATGCAT 1 cut(s) 960
MroNI GCCGGC 1 cut(s) 137
MroXI GAANNNNTTC 1 cut(s) 108
MseI TTAA 1 cut(s) 642
MslI CAYNNNNRTG 2 cut(s) 963, 969
MspA1I CMGCKG 2 cut(s) 16, 1061
MspI CCGG 4 cut(s) 138, 171, 927, 945
MspR9I CCNGG 5 cut(s) 172, 540, 945, 1412, 1724
Mva1269I GAATGC 2 cut(s) 1455, 1606
MvaI CCWGG 3 cut(s) 540, 1412, 1724
MvnI CGCG 2 cut(s) 120, 890
NaeI GCCGGC 1 cut(s) 139
NciI CCSGG 2 cut(s) 172, 945
NdeII GATC 5 cut(s) 363, 831, 1092, 1138, 1734
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 7 cut(s) 28, 424, 799, 962, 968, 1211, 1273
NlaIV GGNNCC 5 cut(s) 315, 534, 802, 942, 1503
NmeAIII GCCGAG 1 cut(s) 881
NmuCI GTSAC 2 cut(s) 628, 744
NsiI ATGCAT 1 cut(s) 960
NspI RCATGY 4 cut(s) 424, 962, 968, 1211
PaeI GCATGC 1 cut(s) 424
PceI AGGCCT 1 cut(s) 1193
PciI ACATGT 2 cut(s) 964, 1207
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PctI GAATGC 2 cut(s) 1455, 1606
PdiI GCCGGC 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 7 cut(s) 109, 122, 151, 236, 281, 287, 609
Pfl23II CGTACG 1 cut(s) 1360
PflFI GACNNNGTC 2 cut(s) 443, 1562
PflMI CCANNNNNTGG 3 cut(s) 105, 1302, 1444
PfoI TCCNGGA 1 cut(s) 1722
PleI GAGTC 4 cut(s) 749, 917, 1350, 1553
PpsI GAGTC 4 cut(s) 749, 917, 1350, 1553
PscI ACATGT 2 cut(s) 964, 1207
PsiI TTATAA 1 cut(s) 1524
Psp124BI GAGCTC 1 cut(s) 900
Psp6I CCWGG 3 cut(s) 538, 1410, 1722
PspFI CCCAGC 3 cut(s) 35, 295, 367
PspGI CCWGG 3 cut(s) 538, 1410, 1722
PspLI CGTACG 1 cut(s) 1360
PspN4I GGNNCC 5 cut(s) 315, 534, 802, 942, 1503
PspPI GGNCC 7 cut(s) 49, 314, 451, 533, 670, 801, 912
PstNI CAGNNNCTG 2 cut(s) 19, 1061
PsyI GACNNNGTC 2 cut(s) 443, 1562
PvuII CAGCTG 2 cut(s) 16, 1061
RsaI GTAC 2 cut(s) 963, 1362
RsaNI GTAC 2 cut(s) 962, 1361
RseI CAYNNNNRTG 2 cut(s) 963, 969
SacI GAGCTC 1 cut(s) 900
SalI GTCGAC 1 cut(s) 386
SaqAI TTAA 1 cut(s) 642
Sau3AI GATC 5 cut(s) 363, 831, 1092, 1138, 1734
Sau96I GGNCC 7 cut(s) 49, 314, 451, 533, 670, 801, 912
SchI GAGTC 4 cut(s) 749, 917, 1350, 1553
ScrFI CCNGG 5 cut(s) 172, 540, 945, 1412, 1724
SduI GDGCHC 4 cut(s) 161, 900, 1258, 1543
SfaNI GCATC 5 cut(s) 945, 1003, 1171, 1273, 1305
SinI GGWCC 1 cut(s) 533
SmiMI CAYNNNNRTG 2 cut(s) 963, 969
SmlI CTYRAG 2 cut(s) 92, 905
SmoI CTYRAG 2 cut(s) 92, 905
SpeI ACTAGT 1 cut(s) 1739
SphI GCATGC 1 cut(s) 424
Sse9I AATT 1 cut(s) 577
SseBI AGGCCT 1 cut(s) 1193
SspMI CTAG 6 cut(s) 59, 144, 359, 1083, 1319, 1740
SstI GAGCTC 1 cut(s) 900
StuI AGGCCT 1 cut(s) 1193
StyD4I CCNGG 5 cut(s) 170, 538, 943, 1410, 1722
StyI CCWWGG 1 cut(s) 1035
TaaI ACNGT 3 cut(s) 709, 1365, 1479
TaiI ACGT 5 cut(s) 247, 352, 447, 511, 651
TaqI TCGA 7 cut(s) 195, 344, 387, 498, 663, 774, 1091
TasI AATT 1 cut(s) 577
TatI WGTACW 1 cut(s) 961
TauI GCSGC 7 cut(s) 50, 178, 302, 692, 841, 917, 1295
TfiI GAWTC 7 cut(s) 109, 122, 151, 236, 281, 287, 609
Tru1I TTAA 1 cut(s) 642
Tru9I TTAA 1 cut(s) 642
TscAI CASTG 5 cut(s) 714, 1258, 1410, 1484, 1681
TseFI GTSAC 2 cut(s) 628, 744
TseI GCWGC 7 cut(s) 13, 16, 62, 852, 1058, 1061, 1070
Tsp45I GTSAC 2 cut(s) 628, 744
TspDTI ATGAA 5 cut(s) 156, 784, 979, 1580, 1634
TspGWI ACGGA 1 cut(s) 1656
TspRI CASTG 5 cut(s) 714, 1258, 1410, 1484, 1681
Tth111I GACNNNGTC 2 cut(s) 443, 1562
Van91I CCANNNNNTGG 3 cut(s) 105, 1302, 1444
VneI GTGCAC 1 cut(s) 1539
VpaK11BI GGWCC 1 cut(s) 533
XceI RCATGY 4 cut(s) 424, 962, 968, 1211
XcmI CCANNNNNNNNNTGG 1 cut(s) 1303
XmiI GTMKAC 1 cut(s) 387
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 6 cut(s) 59, 144, 359, 1083, 1319, 1740
ZraI GACGTC 1 cut(s) 445
Zsp2I ATGCAT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.