pycom04g02220

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011719
Physical Location & Seq
Reverse (-)
1982049 .. 1982925
877 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g02220.2

Sequence Viewer

Length: 441 bp
ATGACAGGAGTAGTGAGGAGGATCAAGTGGGGTTCGCAAGGCCTGGAGTTTTCGGCTCAAGGCCTTGGCTGCATGGGCATGTCCGCCTTCTATAGTGCTCCGAAGCCCGACCAAGACATGATCACTCTCATCCACCACGCCATTGACGCCGGCGTCACCTTCCTCGATACCTCTGACGTCTACGGTCCCTTCACCAACGAAACTCTTCTTGGCAAGGCTCTGAAAGGAGGGGTACGAGATAAGGTGGAATTGGCCACCAAGTTTGGTATCAGACTTGCGGAGGGGTACAAGATGGAGGTGCGAGGTGACCCTGCTTATGTGAGAGCTTCTCTCGAGAGTAGCTTGAAGCGCTTGGGTGTTGATTCTGTTGATCTCTATGATCAGCATCGCATCGACACCTCCATCCCCGTCGAAGTCACGGTTGGTCTTCTTACCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

147

Amino Acids

15.85

Weight (kDa)

5.89

Isoelectric Point (pI)

25.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 1 cut(s) 180
AccI GTMKAC 1 cut(s) 180
AciI CCGC 2 cut(s) 84, 278
AclWI GGATC 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 252
AcyI GRCGYC 3 cut(s) 147, 153, 177
AfaI GTAC 2 cut(s) 234, 287
AfeI AGCGCT 1 cut(s) 350
AgsI TTSAA 1 cut(s) 346
AjnI CCWGG 1 cut(s) 42
AjuI GAANNNNNNNTTGG 4 cut(s) 192, 224, 405, 437
AluBI AGCT 2 cut(s) 326, 342
AluI AGCT 2 cut(s) 326, 342
Alw21I GWGCWC 1 cut(s) 100
AlwI GGATC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 332
Aor51HI AGCGCT 1 cut(s) 350
AoxI GGCC 3 cut(s) 40, 61, 252
ApeKI GCWGC 1 cut(s) 69
Asp700I GAANNNNTTC 1 cut(s) 204
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 3 cut(s) 148, 184, 317
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 1 cut(s) 185
BalI TGGCCA 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 100
BbvI GCAGC 1 cut(s) 56
BccI CCATC 2 cut(s) 286, 410
BciT130I CCWGG 1 cut(s) 44
BclI TGATCA 2 cut(s) 120, 379
BfmI CTRYAG 1 cut(s) 91
BfoI RGCGCY 1 cut(s) 352
BisI GCNGC 1 cut(s) 70
BlsI GCNGC 1 cut(s) 71
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 44
BmsI GCATC 2 cut(s) 394, 399
BpiI GAAGAC 1 cut(s) 419
BplI GAGNNNNNCTC 4 cut(s) 313, 345, 315, 347
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 384
BsaHI GRCGYC 3 cut(s) 147, 153, 177
BsaJI CCNNGG 1 cut(s) 64
Bse118I RCCGGY 1 cut(s) 149
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 2 cut(s) 129, 402
BseJI GATNNNNATC 1 cut(s) 384
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 56
BshFI GGCC 3 cut(s) 42, 63, 254
BsiHKAI GWGCWC 1 cut(s) 100
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 3 cut(s) 42, 63, 254
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 100
Bsp143I GATC 4 cut(s) 21, 120, 370, 379
BspACI CCGC 2 cut(s) 84, 278
BspANI GGCC 3 cut(s) 42, 63, 254
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 1 cut(s) 29
BsrFI RCCGGY 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 4 cut(s) 21, 120, 370, 379
BssNI GRCGYC 3 cut(s) 147, 153, 177
BssT1I CCWWGG 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 185, 421
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 3 cut(s) 147, 153, 177
BstC8I GCNNGC 1 cut(s) 151
BstEII GGTNACC 1 cut(s) 305
BstF5I GGATG 2 cut(s) 129, 402
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 4 cut(s) 24, 123, 373, 382
BstMBI GATC 4 cut(s) 21, 120, 370, 379
BstMWI GCNNNNNNNGC 4 cut(s) 69, 75, 146, 348
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 82
BstPI GGTNACC 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 42
BstSFI CTRYAG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 56
BstV2I GAAGAC 1 cut(s) 419
BsuRI GGCC 3 cut(s) 42, 63, 254
BtgZI GCGATG 1 cut(s) 371
BtsCI GGATG 2 cut(s) 129, 402
Cac8I GCNNGC 1 cut(s) 151
CfoI GCGC 1 cut(s) 351
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 185
CseI GACGC 2 cut(s) 142, 155
Csp6I GTAC 2 cut(s) 233, 286
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 3 cut(s) 73, 79, 118
CviJI RGCY 9 cut(s) 42, 56, 63, 69, 106, 218, 254, 326, 342
CviKI_1 RGCY 9 cut(s) 42, 56, 63, 69, 106, 218, 254, 326, 342
CviQI GTAC 2 cut(s) 233, 286
DpnI GATC 4 cut(s) 23, 122, 372, 381
DpnII GATC 4 cut(s) 21, 120, 370, 379
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 64
Eco147I AGGCCT 2 cut(s) 42, 63
Eco47I GGWCC 1 cut(s) 185
Eco47III AGCGCT 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 332
Eco91I GGTNACC 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 3 cut(s) 76, 82, 121
FaiI YATR 6 cut(s) 74, 80, 93, 119, 318, 378
FaqI GGGAC 1 cut(s) 171
FatI CATG 3 cut(s) 72, 78, 117
FbaI TGATCA 2 cut(s) 120, 379
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 70
FokI GGATG 2 cut(s) 116, 389
Fsp4HI GCNGC 1 cut(s) 70
GlaI GCGC 1 cut(s) 350
GluI GCNGC 1 cut(s) 70
GsuI CTGGAG 1 cut(s) 65
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 3 cut(s) 42, 63, 254
HapII CCGG 1 cut(s) 150
HgaI GACGC 2 cut(s) 142, 155
HhaI GCGC 1 cut(s) 351
Hin1I GRCGYC 3 cut(s) 147, 153, 177
Hin1II CATG 3 cut(s) 76, 82, 121
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HinfI GANTC 1 cut(s) 362
HpaII CCGG 1 cut(s) 150
HphI GGTGA 3 cut(s) 148, 184, 317
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 102, 175, 222, 272
Hpy188III TCNNGA 3 cut(s) 332, 334, 438
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 1 cut(s) 413
HpyAV CCTTC 3 cut(s) 97, 169, 199
HpyCH4III ACNGT 2 cut(s) 185, 421
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 75, 146, 348
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 3 cut(s) 147, 153, 177
Hsp92II CATG 3 cut(s) 76, 82, 121
HspAI GCGC 1 cut(s) 349
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Ksp22I TGATCA 2 cut(s) 120, 379
Kzo9I GATC 4 cut(s) 21, 120, 370, 379
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 4 cut(s) 29, 56, 163, 324
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 2 cut(s) 394, 399
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 3 cut(s) 154, 305, 415
MalI GATC 4 cut(s) 23, 122, 372, 381
MboI GATC 4 cut(s) 21, 120, 370, 379
MboII GAAGA 2 cut(s) 197, 419
MhlI GDGCHC 1 cut(s) 100
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 1 cut(s) 248
MluNI TGGCCA 1 cut(s) 254
MnlI CCTC 9 cut(s) 9, 12, 173, 181, 221, 274, 289, 296, 409
Mox20I TGGCCA 1 cut(s) 254
MreI CGCCGGCG 1 cut(s) 149
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 204
MscI TGGCCA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 254
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 1 cut(s) 44
MvaI CCWGG 1 cut(s) 44
MwoI GCNNNNNNNGC 4 cut(s) 69, 75, 146, 348
NaeI GCCGGC 1 cut(s) 151
NdeII GATC 4 cut(s) 21, 120, 370, 379
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 3 cut(s) 76, 82, 121
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 3 cut(s) 154, 305, 415
NspI RCATGY 1 cut(s) 82
PaeR7I CTCGAG 1 cut(s) 332
PceI AGGCCT 2 cut(s) 42, 63
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 1 cut(s) 362
PkrI GCNGC 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 42
PspEI GGTNACC 1 cut(s) 305
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 1 cut(s) 185
RsaI GTAC 2 cut(s) 234, 287
RsaNI GTAC 2 cut(s) 233, 286
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 1 cut(s) 70
Sau3AI GATC 4 cut(s) 21, 120, 370, 379
Sau96I GGNCC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 44
SduI GDGCHC 1 cut(s) 100
SfaNI GCATC 2 cut(s) 394, 399
SfcI CTRYAG 1 cut(s) 91
Sfr274I CTCGAG 1 cut(s) 332
SgrAI CRCCGGYG 1 cut(s) 149
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 57, 332
SmoI CTYRAG 2 cut(s) 57, 332
Sse9I AATT 1 cut(s) 248
SseBI AGGCCT 2 cut(s) 42, 63
SsiI CCGC 2 cut(s) 84, 278
StuI AGGCCT 2 cut(s) 42, 63
StyD4I CCNGG 1 cut(s) 42
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 2 cut(s) 185, 421
TaiI ACGT 1 cut(s) 180
TaqI TCGA 4 cut(s) 165, 333, 393, 411
TasI AATT 1 cut(s) 248
TfiI GAWTC 1 cut(s) 362
TseFI GTSAC 3 cut(s) 154, 305, 415
TseI GCWGC 1 cut(s) 69
Tsp45I GTSAC 3 cut(s) 154, 305, 415
VpaK11BI GGWCC 1 cut(s) 185
XceI RCATGY 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 1 cut(s) 204
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.