Rh4AG162300

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011719
Physical Location & Seq
Reverse (-)
41161588 .. 41161987
400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG162300.1

Sequence Viewer

Length: 327 bp
ATGGCGGGTGTGAGGAGGATGAAGCTCGGGTCACAAGGCCTGGAGGTCTCAGCCCAAGGACTGGGCTGCATGGGCATGTCAGCCTTCTATGGCCCCCCGAAGCCCGAAGCCGACATGATCAAGCTCATCCACCATGCCGTCGACTCCGGCGTCACCTTCCTCGACACTTCCGACTTCTACGGTCCCCACACCAACGAAATTCTCCTCGGCAATGCTTTGAGTGGAGGGGAGAGAGACAAGGTTGAACTGGCCACCAAATTCGGTATAAGGTTTGCGGACAACAAAATGGTGATTCGAGGTGACCCGGCATATGTGAGAGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

108

Amino Acids

11.59

Weight (kDa)

6.82

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 103 9.2e-14 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 61
AccI GTMKAC 1 cut(s) 141
AciI CCGC 2 cut(s) 5, 275
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 2 cut(s) 198, 257
AcyI GRCGYC 1 cut(s) 150
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 1 cut(s) 245
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 3 cut(s) 25, 124, 320
AluI AGCT 3 cut(s) 25, 124, 320
Alw26I GTCTC 2 cut(s) 52, 228
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 3 cut(s) 37, 91, 249
ApeKI GCWGC 1 cut(s) 66
ApoI RAATTY 2 cut(s) 198, 257
AspS9I GGNCC 2 cut(s) 92, 182
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 3 cut(s) 145, 301, 311
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 251
BbvI GCAGC 1 cut(s) 53
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 2 cut(s) 52, 228
BisI GCNGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 41, 305
Bme18I GGWCC 1 cut(s) 182
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 92, 182
BmiI GGNNCC 2 cut(s) 94, 184
BmrFI CCNGG 2 cut(s) 41, 305
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 305
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 55, 205
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 66, 252
Bse3DI GCAATG 1 cut(s) 217
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 55, 205
BseGI GGATG 2 cut(s) 24, 126
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 2 cut(s) 66, 252
BseRI GAGGAG 2 cut(s) 28, 194
BseXI GCAGC 1 cut(s) 53
BshFI GGCC 3 cut(s) 39, 93, 251
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 2 cut(s) 147, 305
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 2 cut(s) 52, 228
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 3 cut(s) 39, 93, 251
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 26
Bsp143I GATC 1 cut(s) 117
BspACI CCGC 2 cut(s) 5, 275
BspANI GGCC 3 cut(s) 39, 93, 251
BspCNI CTCAG 1 cut(s) 62
BspLI GGNNCC 2 cut(s) 94, 184
BspTNI GGTCTC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 217
BsrI ACTGG 2 cut(s) 66, 252
BssECI CCNNGG 2 cut(s) 55, 205
BssMI GATC 1 cut(s) 117
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 55
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 182
BstACI GRCGYC 1 cut(s) 150
BstDEI CTNAG 1 cut(s) 49
BstEII GGTNACC 1 cut(s) 299
BstF5I GGATG 2 cut(s) 24, 126
BstKTI GATC 1 cut(s) 120
BstMAI GTCTC 2 cut(s) 52, 228
BstMBI GATC 1 cut(s) 117
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 79
BstPI GGTNACC 1 cut(s) 299
BstSCI CCNGG 2 cut(s) 39, 303
BstV1I GCAGC 1 cut(s) 53
BsuRI GGCC 3 cut(s) 39, 93, 251
BtsCI GGATG 2 cut(s) 24, 126
Cfr13I GGNCC 2 cut(s) 92, 182
CseI GACGC 1 cut(s) 139
CviAII CATG 4 cut(s) 70, 76, 115, 134
DdeI CTNAG 1 cut(s) 49
DpnI GATC 1 cut(s) 119
DpnII GATC 1 cut(s) 117
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 26
Eco91I GGTNACC 1 cut(s) 299
EcoO65I GGTNACC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 55
FaeI CATG 4 cut(s) 73, 79, 118, 137
FaiI YATR 8 cut(s) 71, 77, 90, 116, 135, 266, 310, 312
FaqI GGGAC 1 cut(s) 168
FatI CATG 4 cut(s) 69, 75, 114, 133
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 67
FokI GGATG 2 cut(s) 31, 113
Fsp4HI GCNGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 39, 93, 251
HapII CCGG 2 cut(s) 147, 305
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 4 cut(s) 73, 79, 118, 137
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 2 cut(s) 143, 292
HpaII CCGG 2 cut(s) 147, 305
HphI GGTGA 3 cut(s) 145, 301, 311
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 1 cut(s) 172
Hpy188III TCNNGA 1 cut(s) 324
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 94, 166
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpyF3I CTNAG 1 cut(s) 49
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 4 cut(s) 73, 79, 118, 137
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 1 cut(s) 117
LpnPI CCDG 6 cut(s) 26, 47, 53, 160, 233, 318
Lsp1109I GCAGC 1 cut(s) 53
MaeIII GTNAC 3 cut(s) 30, 151, 299
MalI GATC 1 cut(s) 119
MboI GATC 1 cut(s) 117
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 2 cut(s) 198, 257
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 7 cut(s) 6, 9, 37, 170, 215, 218, 290
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 2 cut(s) 147, 305
MspR9I CCNGG 2 cut(s) 41, 305
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 305
NdeI CATATG 1 cut(s) 310
NdeII GATC 1 cut(s) 117
NlaIII CATG 4 cut(s) 73, 79, 118, 137
NlaIV GGNNCC 2 cut(s) 94, 184
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 3 cut(s) 30, 151, 299
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PfeI GAWTC 1 cut(s) 292
PflMI CCANNNNNTGG 1 cut(s) 61
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 39
PspEI GGTNACC 1 cut(s) 299
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 94, 184
PspPI GGNCC 2 cut(s) 92, 182
RseI CAYNNNNRTG 1 cut(s) 74
SalI GTCGAC 1 cut(s) 140
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 1 cut(s) 117
Sau96I GGNCC 2 cut(s) 92, 182
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 41, 305
SetI ASST 8 cut(s) 27, 48, 126, 158, 243, 272, 301, 322
SinI GGWCC 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 74
Sse9I AATT 2 cut(s) 198, 257
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 2 cut(s) 5, 275
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 39, 303
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 182
TaqI TCGA 3 cut(s) 141, 162, 295
TasI AATT 2 cut(s) 198, 257
TfiI GAWTC 1 cut(s) 292
TseFI GTSAC 3 cut(s) 30, 151, 299
TseI GCWGC 1 cut(s) 66
Tsp45I GTSAC 3 cut(s) 30, 151, 299
TspDTI ATGAA 1 cut(s) 35
Van91I CCANNNNNTGG 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 182
XapI RAATTY 2 cut(s) 198, 257
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.