Rmu_sc0029441.1_g000001

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0029441.1
OG0011719
Physical Location & Seq
Reverse (-)
520 .. 997
478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0029441.1_g000001.1.cds

Sequence Viewer

Length: 405 bp
atggcgggtgtgaggaggatgaagcttgggtcacaaggcctggaggtgtcagctcaaggactgggctgcatgggcatgtcagccttctatggccccccgaagcctgaagctgacatgatcaagctcattcaccatgccgtcgactccggcgtcaccttcctcgacacctccgacgtctacggtccccacaccaacgaaattctcctcggcaaggctttgagtggaggggtgagagacaaggttgaactggccaccaaattcggtataaggtttgcggacaacaaaatggtgattcgaggtgacccggcatatgtgagggcttcttgtgaaggaagcttgaaacgacttggtgtagattctgttgatctctattatcagcataggattaatgttttaaaaggctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

134

Amino Acids

14.47

Weight (kDa)

8.93

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AatII GACGTC 1 cut(s) 177
AccI GTMKAC 2 cut(s) 141, 177
AciI CCGC 2 cut(s) 5, 275
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 2 cut(s) 198, 257
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 2 cut(s) 150, 174
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 2 cut(s) 245, 340
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 5 cut(s) 25, 53, 110, 124, 336
AluI AGCT 5 cut(s) 25, 53, 110, 124, 336
Alw26I GTCTC 1 cut(s) 228
AoxI GGCC 3 cut(s) 37, 91, 249
ApeKI GCWGC 1 cut(s) 66
ApoI RAATTY 2 cut(s) 198, 257
AseI ATTAAT 1 cut(s) 387
AspS9I GGNCC 2 cut(s) 92, 182
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 5 cut(s) 122, 145, 241, 301, 311
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 251
BbvI GCAGC 1 cut(s) 53
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 1 cut(s) 228
BisI GCNGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 41, 305
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 92, 182
BmiI GGNNCC 2 cut(s) 94, 184
BmrFI CCNGG 2 cut(s) 41, 305
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 305
BsaHI GRCGYC 2 cut(s) 150, 174
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse1I ACTGG 2 cut(s) 66, 252
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 211
BseNI ACTGG 2 cut(s) 66, 252
BseRI GAGGAG 2 cut(s) 28, 194
BseXI GCAGC 1 cut(s) 53
BshFI GGCC 3 cut(s) 39, 93, 251
BsiSI CCGG 2 cut(s) 147, 305
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 3 cut(s) 39, 93, 251
Bsp143I GATC 2 cut(s) 117, 364
BspACI CCGC 2 cut(s) 5, 275
BspANI GGCC 3 cut(s) 39, 93, 251
BspLI GGNNCC 2 cut(s) 94, 184
BsrI ACTGG 2 cut(s) 66, 252
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 2 cut(s) 117, 364
BssNI GRCGYC 2 cut(s) 150, 174
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 182
BstACI GRCGYC 2 cut(s) 150, 174
BstEII GGTNACC 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 24
BstKTI GATC 2 cut(s) 120, 367
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 2 cut(s) 117, 364
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 79
BstPI GGTNACC 1 cut(s) 299
BstSCI CCNGG 2 cut(s) 39, 303
BstV1I GCAGC 1 cut(s) 53
BsuRI GGCC 3 cut(s) 39, 93, 251
BtsCI GGATG 1 cut(s) 24
Cfr13I GGNCC 2 cut(s) 92, 182
CseI GACGC 1 cut(s) 139
CviAII CATG 4 cut(s) 70, 76, 115, 134
DpnI GATC 2 cut(s) 119, 366
DpnII GATC 2 cut(s) 117, 364
DraI TTTAAA 1 cut(s) 396
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 249
Eco147I AGGCCT 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 126
Eco91I GGTNACC 1 cut(s) 299
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 39
FaeI CATG 4 cut(s) 73, 79, 118, 137
FaiI YATR 9 cut(s) 71, 77, 90, 116, 135, 266, 310, 312, 381
FaqI GGGAC 1 cut(s) 168
FatI CATG 4 cut(s) 69, 75, 114, 133
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 2 cut(s) 141, 177
Fnu4HI GCNGC 1 cut(s) 67
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 39, 93, 251
HapII CCGG 2 cut(s) 147, 305
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 2 cut(s) 150, 174
Hin1II CATG 4 cut(s) 73, 79, 118, 137
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 2 cut(s) 23, 334
HinfI GANTC 3 cut(s) 143, 292, 356
HpaII CCGG 2 cut(s) 147, 305
HphI GGTGA 5 cut(s) 122, 145, 241, 301, 311
Hpy166II GTNNAC 2 cut(s) 142, 178
Hpy188I TCNGA 1 cut(s) 172
Hpy8I GTNNAC 2 cut(s) 142, 178
Hpy99I CGWCG 2 cut(s) 143, 176
HpyAV CCTTC 3 cut(s) 94, 166, 323
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 2 cut(s) 150, 174
Hsp92II CATG 4 cut(s) 73, 79, 118, 137
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 2 cut(s) 117, 364
LpnPI CCDG 7 cut(s) 26, 47, 53, 117, 160, 233, 318
Lsp1109I GCAGC 1 cut(s) 53
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 3 cut(s) 30, 151, 299
MalI GATC 2 cut(s) 119, 366
MboI GATC 2 cut(s) 117, 364
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 2 cut(s) 198, 257
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 9 cut(s) 6, 9, 37, 170, 178, 215, 218, 290, 309
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MseI TTAA 2 cut(s) 387, 395
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 2 cut(s) 147, 305
MspR9I CCNGG 2 cut(s) 41, 305
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 305
NdeI CATATG 1 cut(s) 310
NdeII GATC 2 cut(s) 117, 364
NlaIII CATG 4 cut(s) 73, 79, 118, 137
NlaIV GGNNCC 2 cut(s) 94, 184
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 3 cut(s) 30, 151, 299
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PfeI GAWTC 2 cut(s) 292, 356
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PshBI ATTAAT 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 39
PspEI GGTNACC 1 cut(s) 299
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 94, 184
PspPI GGNCC 2 cut(s) 92, 182
RseI CAYNNNNRTG 1 cut(s) 74
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 2 cut(s) 387, 395
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 2 cut(s) 117, 364
Sau96I GGNCC 2 cut(s) 92, 182
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 41, 305
SinI GGWCC 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 2 cut(s) 198, 257
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 2 cut(s) 5, 275
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 39, 303
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 177
TaqI TCGA 3 cut(s) 141, 162, 295
TasI AATT 2 cut(s) 198, 257
TfiI GAWTC 2 cut(s) 292, 356
Tru1I TTAA 2 cut(s) 387, 395
Tru9I TTAA 2 cut(s) 387, 395
TseFI GTSAC 3 cut(s) 30, 151, 299
TseI GCWGC 1 cut(s) 66
Tsp45I GTSAC 3 cut(s) 30, 151, 299
TspDTI ATGAA 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 182
VspI ATTAAT 1 cut(s) 387
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 2 cut(s) 198, 257
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 2 cut(s) 141, 177
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.