Rh4AG166300

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011719
Physical Location & Seq
Forward (+)
41947040 .. 41980299
33260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG166300.1

Sequence Viewer

Length: 423 bp
ATGGCGGGTGTGAGGAGGATGAAGCTCGGGTCACAAGGCCTGGAGGTCTCAGCACAAGGACTGGGCTGCATGGGCATGTCAGCCTTCTATGGCCCCCCGAAGCCCGAAGCCGACATGATCCAGCTCATCCACCATGCTGTCGACTCCGGCGTCACCTTCCTCGACACTTCCGACGTCTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAATTGGCCACCAAATTCGGTGCCATGTTTGCGGACAACAAAATGGAGATTCGAGGCGAGCCGGCATATGTGAGAGCTTCTTGTGAAGGAAGCTTGAAACGACTTGGTGTAGATTCTATTGATCTCTATTATCAGCATAGGATTGACACCCGTGTCCCCATTGAAGTCACGACTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

140

Amino Acids

15.18

Weight (kDa)

5.78

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 138 7e-26 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 395
AatII GACGTC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 263
AccI GTMKAC 2 cut(s) 141, 177
AciI CCGC 2 cut(s) 5, 275
AclWI GGATC 1 cut(s) 112
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 2 cut(s) 198, 257
AcyI GRCGYC 2 cut(s) 150, 174
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 3 cut(s) 245, 340, 407
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 4 cut(s) 25, 124, 320, 336
AluI AGCT 4 cut(s) 25, 124, 320, 336
Alw26I GTCTC 2 cut(s) 52, 228
AlwI GGATC 1 cut(s) 112
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 3 cut(s) 37, 91, 249
ApeKI GCWGC 1 cut(s) 66
ApoI RAATTY 2 cut(s) 198, 257
AspS9I GGNCC 2 cut(s) 92, 182
AsuHPI GGTGA 2 cut(s) 145, 241
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 251
BanI GGYRCC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 41
BcoDI GTCTC 2 cut(s) 52, 228
BisI GCNGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 1 cut(s) 41
Bme18I GGWCC 1 cut(s) 182
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 92, 182
BmiI GGNNCC 3 cut(s) 94, 184, 265
BmrFI CCNGG 1 cut(s) 41
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 62
BsaHI GRCGYC 2 cut(s) 150, 174
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 2 cut(s) 24, 126
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 66
BseRI GAGGAG 2 cut(s) 28, 194
BseXI GCAGC 1 cut(s) 53
BshFI GGCC 3 cut(s) 39, 93, 251
BshNI GGYRCC 1 cut(s) 263
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 2 cut(s) 147, 305
BslFI GGGAC 2 cut(s) 168, 383
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 2 cut(s) 52, 228
BsmFI GGGAC 2 cut(s) 168, 383
BsnI GGCC 3 cut(s) 39, 93, 251
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 26
Bsp143I GATC 2 cut(s) 117, 364
BspACI CCGC 2 cut(s) 5, 275
BspANI GGCC 3 cut(s) 39, 93, 251
BspCNI CTCAG 1 cut(s) 62
BspLI GGNNCC 3 cut(s) 94, 184, 265
BspPI GGATC 1 cut(s) 112
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 1 cut(s) 66
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 2 cut(s) 117, 364
BssNI GRCGYC 2 cut(s) 150, 174
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 182
BstACI GRCGYC 2 cut(s) 150, 174
BstC8I GCNNGC 2 cut(s) 302, 306
BstDEI CTNAG 1 cut(s) 49
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 2 cut(s) 24, 126
BstKTI GATC 2 cut(s) 120, 367
BstMAI GTCTC 2 cut(s) 52, 228
BstMBI GATC 2 cut(s) 117, 364
BstMWI GCNNNNNNNGC 2 cut(s) 72, 272
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 53
BsuRI GGCC 3 cut(s) 39, 93, 251
BtsCI GGATG 2 cut(s) 24, 126
Cac8I GCNNGC 2 cut(s) 302, 306
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 2 cut(s) 92, 182
CseI GACGC 1 cut(s) 139
CviAII CATG 5 cut(s) 70, 76, 115, 134, 268
DdeI CTNAG 1 cut(s) 49
DpnI GATC 2 cut(s) 119, 366
DpnII GATC 2 cut(s) 117, 364
DrdI GACNNNNNNGTC 2 cut(s) 149, 395
DseDI GACNNNNNNGTC 2 cut(s) 149, 395
EaeI YGGCCR 1 cut(s) 249
Eco147I AGGCCT 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 39
FaeI CATG 5 cut(s) 73, 79, 118, 137, 271
FaiI YATR 9 cut(s) 71, 77, 90, 116, 135, 269, 310, 312, 381
FaqI GGGAC 2 cut(s) 168, 383
FatI CATG 5 cut(s) 69, 75, 114, 133, 267
FauNDI CATATG 1 cut(s) 310
FblI GTMKAC 2 cut(s) 141, 177
Fnu4HI GCNGC 1 cut(s) 67
FokI GGATG 2 cut(s) 31, 113
Fsp4HI GCNGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 39, 93, 251
HapII CCGG 2 cut(s) 147, 305
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 2 cut(s) 150, 174
Hin1II CATG 5 cut(s) 73, 79, 118, 137, 271
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 3 cut(s) 143, 292, 356
HpaII CCGG 2 cut(s) 147, 305
HphI GGTGA 2 cut(s) 145, 241
Hpy166II GTNNAC 2 cut(s) 142, 178
Hpy188I TCNGA 1 cut(s) 172
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 2 cut(s) 142, 178
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 3 cut(s) 94, 166, 323
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 272
HpyF3I CTNAG 1 cut(s) 49
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 2 cut(s) 150, 174
Hsp92II CATG 5 cut(s) 73, 79, 118, 137, 271
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Kzo9I GATC 2 cut(s) 117, 364
LpnPI CCDG 6 cut(s) 26, 47, 53, 134, 160, 318
Lsp1109I GCAGC 1 cut(s) 53
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 3 cut(s) 30, 151, 409
MalI GATC 2 cut(s) 119, 366
MboI GATC 2 cut(s) 117, 364
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 3 cut(s) 198, 245, 257
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 7 cut(s) 6, 9, 37, 170, 215, 218, 290
Mox20I TGGCCA 1 cut(s) 251
MroNI GCCGGC 1 cut(s) 304
MscI TGGCCA 1 cut(s) 251
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 2 cut(s) 147, 305
MspR9I CCNGG 1 cut(s) 41
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 72, 272
NaeI GCCGGC 1 cut(s) 306
NdeI CATATG 1 cut(s) 310
NdeII GATC 2 cut(s) 117, 364
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 5 cut(s) 73, 79, 118, 137, 271
NlaIV GGNNCC 3 cut(s) 94, 184, 265
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 3 cut(s) 30, 151, 409
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PdiI GCCGGC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 292, 356
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 3 cut(s) 94, 184, 265
PspPI GGNCC 2 cut(s) 92, 182
RseI CAYNNNNRTG 1 cut(s) 74
SalI GTCGAC 1 cut(s) 140
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 2 cut(s) 117, 364
Sau96I GGNCC 2 cut(s) 92, 182
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 1 cut(s) 41
SetI ASST 8 cut(s) 27, 48, 126, 158, 177, 243, 322, 338
SinI GGWCC 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 74
Sse9I AATT 3 cut(s) 198, 245, 257
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 2 cut(s) 5, 275
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 177
TaqI TCGA 3 cut(s) 141, 162, 295
TasI AATT 3 cut(s) 198, 245, 257
TfiI GAWTC 2 cut(s) 292, 356
TseFI GTSAC 3 cut(s) 30, 151, 409
TseI GCWGC 1 cut(s) 66
Tsp45I GTSAC 3 cut(s) 30, 151, 409
TspDTI ATGAA 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 182
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 2 cut(s) 198, 257
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 2 cut(s) 141, 177
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.