Rh4AG164400

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011719
Physical Location & Seq
Forward (+)
41598471 .. 41606053
7583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG164400.1

Sequence Viewer

Length: 648 bp
ATGGCGGGTGTGAGGAGGATGAAGCTTGGGTCACAAGGCCTGGAGGTGTCAGCTCAAGGACTGGGCTGCATGGGCATGTCAGCCTTCTATGGCCCCCCGAAGCCCGAAGCCGACATGATCAAGCTCATCCACCATGCCGTCGACTCCGGCGTCACCTTCCTCGACACCTCCGACGTCTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAACTGGCCACCAAATTCGGTATCAGGTTTGCGGACAACAAAATGGTGATTCGAGGTGACCCGGCATATGTGAGAGCTTCTTGTGAAGGAAGCTTGAAACGACTTGGTGTAGATTCTGTTGATCTCTATTATCAGCATAGGATTGACACCCGTGTCCCCATTGAAGTCACGGTACTTCAAAGGATGGTGGATCGATTCCCTCCTTTAAAGGCATTTCATTTTGTCCTTGACGACACTGATGAGAGAGGAAGTGACATTCCACATTCACTTGTTATGTATGCACATCTGATTGTGGAGATCGACAAAACAAAGTTATATGTAGATACTTCCAAAGTAGGCATGAGGTGTAGGATCACCTGGCGAATGCGGGTTTCAGGCCATATGACTCCATTGTTTCTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

215

Amino Acids

24.01

Weight (kDa)

8.94

Isoelectric Point (pI)

22.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 138 7.5e-25 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 395
AatII GACGTC 1 cut(s) 177
AccI GTMKAC 2 cut(s) 141, 177
AciI CCGC 3 cut(s) 5, 275, 610
AclWI GGATC 2 cut(s) 441, 602
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 2 cut(s) 198, 257
AcyI GRCGYC 2 cut(s) 150, 174
AfaI GTAC 1 cut(s) 417
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 4 cut(s) 245, 340, 407, 422
AjnI CCWGG 2 cut(s) 39, 599
AluBI AGCT 5 cut(s) 25, 53, 124, 320, 336
AluI AGCT 5 cut(s) 25, 53, 124, 320, 336
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 2 cut(s) 441, 602
AoxI GGCC 4 cut(s) 37, 91, 249, 619
ApeKI GCWGC 1 cut(s) 66
ApoI RAATTY 2 cut(s) 198, 257
AspS9I GGNCC 2 cut(s) 92, 182
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 5 cut(s) 145, 241, 301, 311, 589
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 251
BarI GAAGNNNNNNTAC 2 cut(s) 399, 431
BbvI GCAGC 1 cut(s) 53
BccI CCATC 1 cut(s) 421
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 2 cut(s) 41, 601
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 1 cut(s) 228
BisI GCNGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 3 cut(s) 41, 305, 601
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 92, 182
BmiI GGNNCC 2 cut(s) 94, 184
BmrFI CCNGG 3 cut(s) 41, 305, 601
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 436
BsaHI GRCGYC 2 cut(s) 150, 174
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse1I ACTGG 2 cut(s) 66, 252
BseBI CCWGG 2 cut(s) 41, 601
BseCI ATCGAT 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 3 cut(s) 24, 126, 432
BseLI CCNNNNNNNGG 1 cut(s) 211
BseNI ACTGG 2 cut(s) 66, 252
BseRI GAGGAG 2 cut(s) 28, 194
BseXI GCAGC 1 cut(s) 53
BshFI GGCC 4 cut(s) 39, 93, 251, 621
BshVI ATCGAT 1 cut(s) 436
BsiSI CCGG 2 cut(s) 147, 305
BslFI GGGAC 2 cut(s) 168, 383
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 2 cut(s) 168, 383
BsmI GAATGC 1 cut(s) 612
BsnI GGCC 4 cut(s) 39, 93, 251, 621
Bsp143I GATC 5 cut(s) 117, 364, 433, 540, 594
BspACI CCGC 3 cut(s) 5, 275, 610
BspANI GGCC 4 cut(s) 39, 93, 251, 621
BspDI ATCGAT 1 cut(s) 436
BspLI GGNNCC 2 cut(s) 94, 184
BspPI GGATC 2 cut(s) 441, 602
BsrI ACTGG 2 cut(s) 66, 252
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 5 cut(s) 117, 364, 433, 540, 594
BssNI GRCGYC 2 cut(s) 150, 174
Bst2UI CCWGG 2 cut(s) 41, 601
Bst4CI ACNGT 2 cut(s) 182, 415
BstACI GRCGYC 2 cut(s) 150, 174
BstEII GGTNACC 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 3 cut(s) 24, 126, 432
BstKTI GATC 5 cut(s) 120, 367, 436, 543, 597
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 5 cut(s) 117, 364, 433, 540, 594
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 2 cut(s) 41, 601
BstNSI RCATGY 1 cut(s) 79
BstPI GGTNACC 1 cut(s) 299
BstSCI CCNGG 3 cut(s) 39, 303, 599
BstV1I GCAGC 1 cut(s) 53
Bsu15I ATCGAT 1 cut(s) 436
BsuRI GGCC 4 cut(s) 39, 93, 251, 621
BsuTUI ATCGAT 1 cut(s) 436
BtsCI GGATG 3 cut(s) 24, 126, 432
BtsIMutI CAGTG 1 cut(s) 477
Cfr13I GGNCC 2 cut(s) 92, 182
ClaI ATCGAT 1 cut(s) 436
CseI GACGC 1 cut(s) 139
Csp6I GTAC 1 cut(s) 416
CviAII CATG 5 cut(s) 70, 76, 115, 134, 583
CviQI GTAC 1 cut(s) 416
DpnI GATC 5 cut(s) 119, 366, 435, 542, 596
DpnII GATC 5 cut(s) 117, 364, 433, 540, 594
DraI TTTAAA 1 cut(s) 450
DrdI GACNNNNNNGTC 2 cut(s) 149, 395
DseDI GACNNNNNNGTC 2 cut(s) 149, 395
EaeI YGGCCR 1 cut(s) 249
Eco147I AGGCCT 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 299
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 39, 599
FaeI CATG 5 cut(s) 73, 79, 118, 137, 586
FaqI GGGAC 2 cut(s) 168, 383
FatI CATG 5 cut(s) 69, 75, 114, 133, 582
FauI CCCGC 1 cut(s) 603
FauNDI CATATG 2 cut(s) 310, 624
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 2 cut(s) 141, 177
Fnu4HI GCNGC 1 cut(s) 67
FokI GGATG 3 cut(s) 31, 113, 439
Fsp4HI GCNGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 4 cut(s) 39, 93, 251, 621
HapII CCGG 2 cut(s) 147, 305
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 2 cut(s) 150, 174
Hin1II CATG 5 cut(s) 73, 79, 118, 137, 586
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 2 cut(s) 23, 334
HinfI GANTC 5 cut(s) 143, 292, 356, 438, 628
HpaII CCGG 2 cut(s) 147, 305
HphI GGTGA 5 cut(s) 145, 241, 301, 311, 589
Hpy166II GTNNAC 2 cut(s) 142, 178
Hpy188I TCNGA 3 cut(s) 172, 531, 642
Hpy8I GTNNAC 2 cut(s) 142, 178
Hpy99I CGWCG 2 cut(s) 143, 176
HpyAV CCTTC 3 cut(s) 94, 166, 323
HpyCH4III ACNGT 2 cut(s) 182, 415
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 2 cut(s) 69, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 2 cut(s) 150, 174
Hsp92II CATG 5 cut(s) 73, 79, 118, 137, 586
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 5 cut(s) 117, 364, 433, 540, 594
Lsp1109I GCAGC 1 cut(s) 53
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 5 cut(s) 30, 151, 299, 409, 494
MalI GATC 5 cut(s) 119, 366, 435, 542, 596
MboI GATC 5 cut(s) 117, 364, 433, 540, 594
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 2 cut(s) 198, 257
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 2 cut(s) 137, 622
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MseI TTAA 1 cut(s) 449
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 2 cut(s) 147, 305
MspR9I CCNGG 3 cut(s) 41, 305, 601
Mva1269I GAATGC 1 cut(s) 612
MvaI CCWGG 2 cut(s) 41, 601
MwoI GCNNNNNNNGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 305
NdeI CATATG 2 cut(s) 310, 624
NdeII GATC 5 cut(s) 117, 364, 433, 540, 594
NlaIII CATG 5 cut(s) 73, 79, 118, 137, 586
NlaIV GGNNCC 2 cut(s) 94, 184
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 5 cut(s) 30, 151, 299, 409, 494
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PctI GAATGC 1 cut(s) 612
PfeI GAWTC 3 cut(s) 292, 356, 438
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 2 cut(s) 137, 622
PpsI GAGTC 2 cut(s) 137, 622
Psp6I CCWGG 2 cut(s) 39, 599
PspEI GGTNACC 1 cut(s) 299
PspGI CCWGG 2 cut(s) 39, 599
PspN4I GGNNCC 2 cut(s) 94, 184
PspPI GGNCC 2 cut(s) 92, 182
RsaI GTAC 1 cut(s) 417
RsaNI GTAC 1 cut(s) 416
RseI CAYNNNNRTG 1 cut(s) 74
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 5 cut(s) 117, 364, 433, 540, 594
Sau96I GGNCC 2 cut(s) 92, 182
SchI GAGTC 2 cut(s) 137, 622
ScrFI CCNGG 3 cut(s) 41, 305, 601
SinI GGWCC 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 2 cut(s) 198, 257
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 3 cut(s) 5, 275, 610
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 3 cut(s) 39, 303, 599
TaaI ACNGT 2 cut(s) 182, 415
TaiI ACGT 1 cut(s) 177
TaqI TCGA 5 cut(s) 141, 162, 295, 436, 543
TasI AATT 2 cut(s) 198, 257
TfiI GAWTC 3 cut(s) 292, 356, 438
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 5 cut(s) 30, 151, 299, 409, 494
TseI GCWGC 1 cut(s) 66
Tsp45I GTSAC 5 cut(s) 30, 151, 299, 409, 494
TspDTI ATGAA 2 cut(s) 35, 449
TspRI CASTG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 182
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 2 cut(s) 198, 257
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 2 cut(s) 141, 177
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.