pycom13g29360

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011719
Physical Location & Seq
Reverse (-)
25970206 .. 25973514
3309 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g29360.1

Sequence Viewer

Length: 870 bp
ATGATATTTACGGGTAGTACTAAAGGCTCCAAGCCTCGAAACTATTATAAAATCAATAATGCTATTTTGATTTCACTTAATCTGCTTACACAAATGGCAGGAGGAGCAGGAATAGTGAAGAGGATGAAGTTGGGTTCACAAGGCCTCGAGGTGTCGGCTCAAGGCCTTGGCTGCATGGGCATGTCTGCCTTCTACGGCGCTCCAAAGCCCGACCAAGACATGATCGATCTCATCCACCATGCCATTAACAGCGGCGTCACCTTCCTTGATACCTCTGACATCTACGGTCCCTTCACCAACGAAATCCTTCTTGGCAAGGCTCTCAAGGGAGGGGTACGAGACAAGGTTGAATTGGCCACCAAGTTTGGTGTCAGATTTGCGGAGAACAAGATGGAGGTGCAAGGTGACCCTGCTTATGTGAGAGCTGCTCTCGAGAGTAGCTTGAAGCGCCTTGGTGTTGATTCTATTGATCTCTATTATCAGCATCGCATTGACACCTCCGTCCCCATTGAAGTCACGGGTAAGGTAAAGTACATTGGTCTATCCGAGGCCTCAGCTTCAACGATTAGAGGAGCCCATGATGTTCATCCTATAACAGCTGTGCAGTTGGAGTGGTCCTCGTGGTCAAGAGATGTCGAGCAAGAAATAATTCCTACTTGTCGGGAGCTTGGCATTGGTATTGTTGCGTATAGTCCTTTAGGAAGAGGATTCTTCTCATCAGGTGCTAAGTTTGTTGAAAACCTTGCCCACGATGATTCCCGAAAGAATCTACCGAGGTTCCAACCCGAAAATGTAGAGCACAACAAATCACTATTCGAGCGAGTTAGTTCGATCTTGCAACAAGGAAAGGGTGCACACCATCTCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

290

Amino Acids

31.57

Weight (kDa)

8.4

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 58 - 278 4.5e-45 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
AasI GACNNNNNNGTC 1 cut(s) 500
AbsI CCTCGAGG 1 cut(s) 146
AciI CCGC 2 cut(s) 252, 380
AcoI YGGCCR 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 3 cut(s) 19, 336, 533
AgsI TTSAA 5 cut(s) 350, 445, 512, 561, 737
AjuI GAANNNNNNNTTGG 2 cut(s) 294, 326
AluBI AGCT 6 cut(s) 425, 441, 557, 599, 667, 867
AluI AGCT 6 cut(s) 425, 441, 557, 599, 667, 867
Alw21I GWGCWC 2 cut(s) 801, 856
Alw26I GTCTC 1 cut(s) 333
Alw44I GTGCAC 1 cut(s) 852
Ama87I CYCGRG 2 cut(s) 146, 431
AoxI GGCC 4 cut(s) 142, 163, 354, 549
ApaLI GTGCAC 1 cut(s) 852
ApeKI GCWGC 2 cut(s) 171, 425
Asp700I GAANNNNTTC 2 cut(s) 306, 648
AspLEI GCGC 2 cut(s) 200, 450
AspS9I GGNCC 2 cut(s) 287, 615
AsuHPI GGTGA 3 cut(s) 250, 286, 416
AvaI CYCGRG 2 cut(s) 146, 431
AvaII GGWCC 2 cut(s) 287, 615
BaeGI GKGCMC 1 cut(s) 856
BalI TGGCCA 1 cut(s) 356
BanII GRGCYC 1 cut(s) 577
BauI CACGAG 1 cut(s) 619
Bbv12I GWGCWC 2 cut(s) 801, 856
BbvCI CCTCAGC 1 cut(s) 553
BbvI GCAGC 2 cut(s) 158, 412
BccI CCATC 2 cut(s) 385, 867
BceAI ACGGC 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 1 cut(s) 868
BfoI RGCGCY 2 cut(s) 201, 451
BisI GCNGC 3 cut(s) 172, 253, 426
BlsI GCNGC 3 cut(s) 173, 254, 427
BmcAI AGTACT 1 cut(s) 19
Bme18I GGWCC 2 cut(s) 287, 615
BmeT110I CYCGRG 2 cut(s) 146, 431
BmgT120I GGNCC 2 cut(s) 287, 615
BmiI GGNNCC 4 cut(s) 28, 289, 574, 779
BmsI GCATC 1 cut(s) 493
BplI GAGNNNNNCTC 6 cut(s) 412, 444, 414, 446, 602, 634
Bpu10I CCTNAGC 1 cut(s) 553
BpuEI CTTGAG 2 cut(s) 144, 308
Bsa29I ATCGAT 1 cut(s) 225
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 4 cut(s) 166, 451, 546, 773
Bse8I GATNNNNATC 1 cut(s) 585
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 4 cut(s) 166, 451, 546, 773
BseGI GGATG 3 cut(s) 129, 231, 586
BseJI GATNNNNATC 1 cut(s) 585
BseMII CTCAG 1 cut(s) 567
BseRI GAGGAG 2 cut(s) 117, 585
BseSI GKGCMC 1 cut(s) 856
BseXI GCAGC 2 cut(s) 158, 412
BsgI GTGCAG 1 cut(s) 623
BshFI GGCC 4 cut(s) 144, 165, 356, 551
BshVI ATCGAT 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 801, 856
BsiHKCI CYCGRG 2 cut(s) 146, 431
BslFI GGGAC 2 cut(s) 273, 488
BsmAI GTCTC 1 cut(s) 333
BsmFI GGGAC 2 cut(s) 273, 488
BsnI GGCC 4 cut(s) 144, 165, 356, 551
BsoBI CYCGRG 2 cut(s) 146, 431
Bsp1286I GDGCHC 3 cut(s) 577, 801, 856
Bsp143I GATC 4 cut(s) 222, 226, 469, 831
BspACI CCGC 2 cut(s) 252, 380
BspANI GGCC 4 cut(s) 144, 165, 356, 551
BspCNI CTCAG 1 cut(s) 566
BspDI ATCGAT 1 cut(s) 225
BspLI GGNNCC 4 cut(s) 28, 289, 574, 779
BssECI CCNNGG 4 cut(s) 166, 451, 546, 773
BssMI GATC 4 cut(s) 222, 226, 469, 831
BssNI GRCGYC 1 cut(s) 255
BssSI CACGAG 1 cut(s) 619
BssT1I CCWWGG 2 cut(s) 166, 451
Bst2BI CACGAG 1 cut(s) 619
Bst4CI ACNGT 1 cut(s) 287
Bst6I CTCTTC 2 cut(s) 113, 697
BstACI GRCGYC 1 cut(s) 255
BstDEI CTNAG 3 cut(s) 553, 726, 863
BstEII GGTNACC 1 cut(s) 404
BstF5I GGATG 3 cut(s) 129, 231, 586
BstH2I RGCGCY 2 cut(s) 201, 451
BstHHI GCGC 2 cut(s) 200, 450
BstKTI GATC 4 cut(s) 225, 229, 472, 834
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 4 cut(s) 222, 226, 469, 831
BstMWI GCNNNNNNNGC 4 cut(s) 104, 171, 177, 447
BstNSI RCATGY 1 cut(s) 184
BstPI GGTNACC 1 cut(s) 404
BstSLI GKGCMC 1 cut(s) 856
BstV1I GCAGC 2 cut(s) 158, 412
Bsu15I ATCGAT 1 cut(s) 225
BsuRI GGCC 4 cut(s) 144, 165, 356, 551
BsuTUI ATCGAT 1 cut(s) 225
BtgZI GCGATG 1 cut(s) 470
BtsCI GGATG 3 cut(s) 129, 231, 586
CfoI GCGC 2 cut(s) 200, 450
Cfr13I GGNCC 2 cut(s) 287, 615
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 1 cut(s) 244
Csp6I GTAC 3 cut(s) 18, 335, 532
CspCI CAANNNNNGTGG 2 cut(s) 346, 381
CviAII CATG 5 cut(s) 175, 181, 220, 239, 578
CviQI GTAC 3 cut(s) 18, 335, 532
DdeI CTNAG 3 cut(s) 553, 726, 863
DpnI GATC 4 cut(s) 224, 228, 471, 833
DpnII GATC 4 cut(s) 222, 226, 469, 831
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 113, 697
EarI CTCTTC 2 cut(s) 113, 697
Eco130I CCWWGG 2 cut(s) 166, 451
Eco147I AGGCCT 3 cut(s) 144, 165, 551
Eco24I GRGCYC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 287, 615
Eco88I CYCGRG 2 cut(s) 146, 431
Eco91I GGTNACC 1 cut(s) 404
EcoO65I GGTNACC 1 cut(s) 404
EcoT14I CCWWGG 2 cut(s) 166, 451
EcoT38I GRGCYC 1 cut(s) 577
ErhI CCWWGG 2 cut(s) 166, 451
FaeI CATG 5 cut(s) 178, 184, 223, 242, 581
FaiI YATR 9 cut(s) 48, 176, 182, 221, 240, 417, 579, 593, 690
FaqI GGGAC 2 cut(s) 273, 488
FatI CATG 5 cut(s) 174, 180, 219, 238, 577
Fnu4HI GCNGC 3 cut(s) 172, 253, 426
FokI GGATG 3 cut(s) 136, 218, 573
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 3 cut(s) 172, 253, 426
FspBI CTAG 1 cut(s) 868
GlaI GCGC 2 cut(s) 199, 449
GluI GCNGC 3 cut(s) 172, 253, 426
HaeII RGCGCY 2 cut(s) 201, 451
HaeIII GGCC 4 cut(s) 144, 165, 356, 551
HgaI GACGC 1 cut(s) 244
HhaI GCGC 2 cut(s) 200, 450
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 5 cut(s) 178, 184, 223, 242, 581
Hin6I GCGC 2 cut(s) 198, 448
HinP1I GCGC 2 cut(s) 198, 448
HinfI GANTC 4 cut(s) 461, 708, 755, 766
HphI GGTGA 3 cut(s) 250, 286, 416
Hpy166II GTNNAC 2 cut(s) 137, 854
Hpy188I TCNGA 3 cut(s) 277, 374, 547
Hpy188III TCNNGA 5 cut(s) 431, 433, 627, 662, 759
Hpy8I GTNNAC 2 cut(s) 137, 854
HpyAV CCTTC 4 cut(s) 199, 271, 301, 317
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4V TGCA 5 cut(s) 174, 400, 604, 838, 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 171, 177, 447
HpyF3I CTNAG 3 cut(s) 553, 726, 863
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 5 cut(s) 178, 184, 223, 242, 581
HspAI GCGC 2 cut(s) 198, 448
Kzo9I GATC 4 cut(s) 222, 226, 469, 831
LmnI GCTCC 5 cut(s) 32, 104, 205, 572, 664
LpnPI CCDG 4 cut(s) 84, 93, 423, 705
Lsp1109I GCAGC 2 cut(s) 158, 412
LweI GCATC 1 cut(s) 493
MaeI CTAG 1 cut(s) 868
MaeIII GTNAC 3 cut(s) 256, 404, 514
MalI GATC 4 cut(s) 224, 228, 471, 833
MboI GATC 4 cut(s) 222, 226, 469, 831
MboII GAAGA 3 cut(s) 130, 703, 714
MhlI GDGCHC 3 cut(s) 577, 801, 856
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 2 cut(s) 350, 648
MluNI TGGCCA 1 cut(s) 356
MmeI TCCRAC 2 cut(s) 588, 805
Mox20I TGGCCA 1 cut(s) 356
MroXI GAANNNNTTC 2 cut(s) 306, 648
MscI TGGCCA 1 cut(s) 356
MseI TTAA 2 cut(s) 78, 246
MslI CAYNNNNRTG 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 2 cut(s) 252, 599
MwoI GCNNNNNNNGC 4 cut(s) 104, 171, 177, 447
NdeII GATC 4 cut(s) 222, 226, 469, 831
NlaIII CATG 5 cut(s) 178, 184, 223, 242, 581
NlaIV GGNNCC 4 cut(s) 28, 289, 574, 779
NmuCI GTSAC 3 cut(s) 256, 404, 514
NspI RCATGY 1 cut(s) 184
PaeR7I CTCGAG 2 cut(s) 146, 431
PceI AGGCCT 3 cut(s) 144, 165, 551
PdmI GAANNNNTTC 2 cut(s) 306, 648
PfeI GAWTC 4 cut(s) 461, 708, 755, 766
PkrI GCNGC 3 cut(s) 173, 254, 427
PsiI TTATAA 1 cut(s) 48
PspEI GGTNACC 1 cut(s) 404
PspN4I GGNNCC 4 cut(s) 28, 289, 574, 779
PspPI GGNCC 2 cut(s) 287, 615
PspXI VCTCGAGB 1 cut(s) 146
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 3 cut(s) 19, 336, 533
RsaNI GTAC 3 cut(s) 18, 335, 532
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 2 cut(s) 78, 246
SatI GCNGC 3 cut(s) 172, 253, 426
Sau3AI GATC 4 cut(s) 222, 226, 469, 831
Sau96I GGNCC 2 cut(s) 287, 615
ScaI AGTACT 1 cut(s) 19
SduI GDGCHC 3 cut(s) 577, 801, 856
SfaNI GCATC 1 cut(s) 493
Sfr274I CTCGAG 2 cut(s) 146, 431
SinI GGWCC 2 cut(s) 287, 615
SlaI CTCGAG 2 cut(s) 146, 431
SmiMI CAYNNNNRTG 1 cut(s) 179
SmlI CTYRAG 4 cut(s) 146, 159, 323, 431
SmoI CTYRAG 4 cut(s) 146, 159, 323, 431
Sse9I AATT 2 cut(s) 350, 648
SseBI AGGCCT 3 cut(s) 144, 165, 551
SsiI CCGC 2 cut(s) 252, 380
SspMI CTAG 1 cut(s) 868
StuI AGGCCT 3 cut(s) 144, 165, 551
StyI CCWWGG 2 cut(s) 166, 451
TaaI ACNGT 1 cut(s) 287
TaqI TCGA 7 cut(s) 37, 147, 225, 432, 636, 816, 830
TasI AATT 2 cut(s) 350, 648
TatI WGTACW 2 cut(s) 17, 531
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 4 cut(s) 461, 708, 755, 766
Tru1I TTAA 2 cut(s) 78, 246
Tru9I TTAA 2 cut(s) 78, 246
TseFI GTSAC 3 cut(s) 256, 404, 514
TseI GCWGC 2 cut(s) 171, 425
Tsp45I GTSAC 3 cut(s) 256, 404, 514
TspDTI ATGAA 2 cut(s) 140, 575
TspGWI ACGGA 1 cut(s) 490
VneI GTGCAC 1 cut(s) 852
VpaK11BI GGWCC 2 cut(s) 287, 615
XceI RCATGY 1 cut(s) 184
XhoI CTCGAG 2 cut(s) 146, 431
XmnI GAANNNNTTC 2 cut(s) 306, 648
XspI CTAG 1 cut(s) 868
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.