pycom04g02220

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011719
Physical Location & Seq
Reverse (-)
1982049 .. 1982925
877 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 441 bp
ATGACAGGAGTAGTGAGGAGGATCAAGTGGGGTTCGCAAGGCCTGGAGTTTTCGGCTCAAGGCCTTGGCTGCATGGGCATGTCCGCCTTCTATAGTGCTCCGAAGCCCGACCAAGACATGATCACTCTCATCCACCACGCCATTGACGCCGGCGTCACCTTCCTCGATACCTCTGACGTCTACGGTCCCTTCACCAACGAAACTCTTCTTGGCAAGGCTCTGAAAGGAGGGGTACGAGATAAGGTGGAATTGGCCACCAAGTTTGGTATCAGACTTGCGGAGGGGTACAAGATGGAGGTGCGAGGTGACCCTGCTTATGTGAGAGCTTCTCTCGAGAGTAGCTTGAAGCGCTTGGGTGTTGATTCTGTTGATCTCTATGATCAGCATCGCATCGACACCTCCATCCCCGTCGAAGTCACGGTTGGTCTTCTTACCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

147

Amino Acids

15.85

Weight (kDa)

5.89

Isoelectric Point (pI)

25.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 24 - 144 1.7e-24 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 1 cut(s) 180
AccI GTMKAC 1 cut(s) 180
AciI CCGC 2 cut(s) 84, 278
AclWI GGATC 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 252
AcyI GRCGYC 3 cut(s) 147, 153, 177
AfaI GTAC 2 cut(s) 234, 287
AfeI AGCGCT 1 cut(s) 350
AgsI TTSAA 1 cut(s) 346
AjnI CCWGG 1 cut(s) 42
AjuI GAANNNNNNNTTGG 4 cut(s) 192, 224, 405, 437
AluBI AGCT 2 cut(s) 326, 342
AluI AGCT 2 cut(s) 326, 342
Alw21I GWGCWC 1 cut(s) 100
AlwI GGATC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 332
Aor51HI AGCGCT 1 cut(s) 350
AoxI GGCC 3 cut(s) 40, 61, 252
ApeKI GCWGC 1 cut(s) 69
Asp700I GAANNNNTTC 1 cut(s) 204
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 3 cut(s) 148, 184, 317
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 1 cut(s) 185
BalI TGGCCA 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 100
BbvI GCAGC 1 cut(s) 56
BccI CCATC 2 cut(s) 286, 410
BciT130I CCWGG 1 cut(s) 44
BclI TGATCA 2 cut(s) 120, 379
BfmI CTRYAG 1 cut(s) 91
BfoI RGCGCY 1 cut(s) 352
BisI GCNGC 1 cut(s) 70
BlsI GCNGC 1 cut(s) 71
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 44
BmsI GCATC 2 cut(s) 394, 399
BpiI GAAGAC 1 cut(s) 419
BplI GAGNNNNNCTC 4 cut(s) 313, 345, 315, 347
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 384
BsaHI GRCGYC 3 cut(s) 147, 153, 177
BsaJI CCNNGG 1 cut(s) 64
Bse118I RCCGGY 1 cut(s) 149
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 2 cut(s) 129, 402
BseJI GATNNNNATC 1 cut(s) 384
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 56
BshFI GGCC 3 cut(s) 42, 63, 254
BsiHKAI GWGCWC 1 cut(s) 100
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 3 cut(s) 42, 63, 254
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 100
Bsp143I GATC 4 cut(s) 21, 120, 370, 379
BspACI CCGC 2 cut(s) 84, 278
BspANI GGCC 3 cut(s) 42, 63, 254
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 1 cut(s) 29
BsrFI RCCGGY 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 4 cut(s) 21, 120, 370, 379
BssNI GRCGYC 3 cut(s) 147, 153, 177
BssT1I CCWWGG 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 185, 421
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 3 cut(s) 147, 153, 177
BstC8I GCNNGC 1 cut(s) 151
BstEII GGTNACC 1 cut(s) 305
BstF5I GGATG 2 cut(s) 129, 402
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 4 cut(s) 24, 123, 373, 382
BstMBI GATC 4 cut(s) 21, 120, 370, 379
BstMWI GCNNNNNNNGC 4 cut(s) 69, 75, 146, 348
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 82
BstPI GGTNACC 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 42
BstSFI CTRYAG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 56
BstV2I GAAGAC 1 cut(s) 419
BsuRI GGCC 3 cut(s) 42, 63, 254
BtgZI GCGATG 1 cut(s) 371
BtsCI GGATG 2 cut(s) 129, 402
Cac8I GCNNGC 1 cut(s) 151
CfoI GCGC 1 cut(s) 351
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 185
CseI GACGC 2 cut(s) 142, 155
Csp6I GTAC 2 cut(s) 233, 286
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 3 cut(s) 73, 79, 118
CviJI RGCY 9 cut(s) 42, 56, 63, 69, 106, 218, 254, 326, 342
CviKI_1 RGCY 9 cut(s) 42, 56, 63, 69, 106, 218, 254, 326, 342
CviQI GTAC 2 cut(s) 233, 286
DpnI GATC 4 cut(s) 23, 122, 372, 381
DpnII GATC 4 cut(s) 21, 120, 370, 379
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 64
Eco147I AGGCCT 2 cut(s) 42, 63
Eco47I GGWCC 1 cut(s) 185
Eco47III AGCGCT 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 332
Eco91I GGTNACC 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 3 cut(s) 76, 82, 121
FaiI YATR 6 cut(s) 74, 80, 93, 119, 318, 378
FaqI GGGAC 1 cut(s) 171
FatI CATG 3 cut(s) 72, 78, 117
FbaI TGATCA 2 cut(s) 120, 379
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 70
FokI GGATG 2 cut(s) 116, 389
Fsp4HI GCNGC 1 cut(s) 70
GlaI GCGC 1 cut(s) 350
GluI GCNGC 1 cut(s) 70
GsuI CTGGAG 1 cut(s) 65
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 3 cut(s) 42, 63, 254
HapII CCGG 1 cut(s) 150
HgaI GACGC 2 cut(s) 142, 155
HhaI GCGC 1 cut(s) 351
Hin1I GRCGYC 3 cut(s) 147, 153, 177
Hin1II CATG 3 cut(s) 76, 82, 121
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HinfI GANTC 1 cut(s) 362
HpaII CCGG 1 cut(s) 150
HphI GGTGA 3 cut(s) 148, 184, 317
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 102, 175, 222, 272
Hpy188III TCNNGA 3 cut(s) 332, 334, 438
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 1 cut(s) 413
HpyAV CCTTC 3 cut(s) 97, 169, 199
HpyCH4III ACNGT 2 cut(s) 185, 421
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 75, 146, 348
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 3 cut(s) 147, 153, 177
Hsp92II CATG 3 cut(s) 76, 82, 121
HspAI GCGC 1 cut(s) 349
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Ksp22I TGATCA 2 cut(s) 120, 379
Kzo9I GATC 4 cut(s) 21, 120, 370, 379
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 4 cut(s) 29, 56, 163, 324
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 2 cut(s) 394, 399
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 3 cut(s) 154, 305, 415
MalI GATC 4 cut(s) 23, 122, 372, 381
MboI GATC 4 cut(s) 21, 120, 370, 379
MboII GAAGA 2 cut(s) 197, 419
MhlI GDGCHC 1 cut(s) 100
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 1 cut(s) 248
MluNI TGGCCA 1 cut(s) 254
MnlI CCTC 9 cut(s) 9, 12, 173, 181, 221, 274, 289, 296, 409
Mox20I TGGCCA 1 cut(s) 254
MreI CGCCGGCG 1 cut(s) 149
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 204
MscI TGGCCA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 254
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 1 cut(s) 44
MvaI CCWGG 1 cut(s) 44
MwoI GCNNNNNNNGC 4 cut(s) 69, 75, 146, 348
NaeI GCCGGC 1 cut(s) 151
NdeII GATC 4 cut(s) 21, 120, 370, 379
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 3 cut(s) 76, 82, 121
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 3 cut(s) 154, 305, 415
NspI RCATGY 1 cut(s) 82
PaeR7I CTCGAG 1 cut(s) 332
PceI AGGCCT 2 cut(s) 42, 63
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 1 cut(s) 362
PkrI GCNGC 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 42
PspEI GGTNACC 1 cut(s) 305
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 1 cut(s) 185
RsaI GTAC 2 cut(s) 234, 287
RsaNI GTAC 2 cut(s) 233, 286
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 1 cut(s) 70
Sau3AI GATC 4 cut(s) 21, 120, 370, 379
Sau96I GGNCC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 44
SduI GDGCHC 1 cut(s) 100
SfaNI GCATC 2 cut(s) 394, 399
SfcI CTRYAG 1 cut(s) 91
Sfr274I CTCGAG 1 cut(s) 332
SgrAI CRCCGGYG 1 cut(s) 149
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 57, 332
SmoI CTYRAG 2 cut(s) 57, 332
Sse9I AATT 1 cut(s) 248
SseBI AGGCCT 2 cut(s) 42, 63
SsiI CCGC 2 cut(s) 84, 278
StuI AGGCCT 2 cut(s) 42, 63
StyD4I CCNGG 1 cut(s) 42
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 2 cut(s) 185, 421
TaiI ACGT 1 cut(s) 180
TaqI TCGA 4 cut(s) 165, 333, 393, 411
TasI AATT 1 cut(s) 248
TfiI GAWTC 1 cut(s) 362
TseFI GTSAC 3 cut(s) 154, 305, 415
TseI GCWGC 1 cut(s) 69
Tsp45I GTSAC 3 cut(s) 154, 305, 415
VpaK11BI GGWCC 1 cut(s) 185
XceI RCATGY 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 1 cut(s) 204
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.