pycom04g04120

methionine sulfoxide reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007363
Physical Location & Seq
Reverse (-)
3680335 .. 3681889
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGGCTTTTCTTCACAGCAGCATTTCCCTTAGCCGTCTATTAACAACATTCGTGCTCCTGACAATTCTTACGGCCGAGAGACGCGTGTCCATCAGGATCCCAGATCGGATCACTGAAACCACAAGAGACCTACCTGACAACCAATCCCTGAAAACAGCAGTTTTCGCTCTGGGAAGCTTTTGGAGATCCGAAGCTGTCTTTGGGTGCTTGAATGGAGTTGTACAGACGACTGTTGGTTATGCTGGCGGATCCAAAACCAACCCTGAGTACAGAAGCTTGGGTGATCATGCCGAATCTATCCAGGTTGTATATGATCACAGGCGGATTAGCTTCAAGCAACTTTTGGAGGTTTTCTGGTCCAGTCATGATTATAGGCAAGTATTTGGGCAAGGTCCTGATGTGGGTAACCAATACAGATCTATCATTTTTACGAATGGAACTGAAGAGTCCAGATTGGCCGCTACGAGCAAAGAGAGAGAGCAAACTAAGTCAAAAAGTAGCAGTGTAACTACTAAAATCCTACCTCTGGGATCGTTTTATCCTGCTGAGCCTGAACATCAGAAGTTTGAGCTGAAACGTAGGCCATTTCTTCTCCAGTTAATTGGAAACTTGCCGGAAGAGGAACTTGAGAGGTCAAATTTGGCTGCAAAACTGAATGGATATGCTGCAGAGCTTTGCCCTCCGAAAATCCAGAGACAGATTGATGCAAAAATCAACGAAATTATTAAAAAGGGATGGCCCATTTTGAGAGACCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMSR

Protein Analysis

253

Amino Acids

28.51

Weight (kDa)

9.18

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMSR PF01625 52 - 192 7.7e-44 Peptide methionine sulfoxide reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 84
AciI CCGC 3 cut(s) 246, 322, 459
AclWI GGATC 7 cut(s) 91, 104, 116, 180, 243, 256, 538
AcoI YGGCCR 2 cut(s) 72, 456
AcsI RAATTY 1 cut(s) 637
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 2 cut(s) 222, 269
AfiI CCNNNNNNNGG 2 cut(s) 401, 526
AflIII ACRYGT 1 cut(s) 82
AgsI TTSAA 2 cut(s) 211, 334
AjnI CCWGG 1 cut(s) 300
AjuI GAANNNNNNNTTGG 2 cut(s) 183, 215
AluBI AGCT 6 cut(s) 177, 194, 276, 330, 571, 673
AluI AGCT 6 cut(s) 177, 194, 276, 330, 571, 673
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 4 cut(s) 73, 120, 688, 744
AlwI GGATC 7 cut(s) 91, 104, 116, 180, 243, 256, 538
AoxI GGCC 4 cut(s) 72, 456, 581, 737
ApeKI GCWGC 3 cut(s) 18, 644, 665
ApoI RAATTY 1 cut(s) 637
AspS9I GGNCC 3 cut(s) 357, 392, 738
AsuHPI GGTGA 1 cut(s) 293
AvaII GGWCC 2 cut(s) 357, 392
BamHI GGATCC 2 cut(s) 96, 248
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 3 cut(s) 30, 631, 652
BccI CCATC 2 cut(s) 98, 729
BceAI ACGGC 2 cut(s) 18, 87
BciT130I CCWGG 1 cut(s) 302
BclI TGATCA 2 cut(s) 283, 313
BcoDI GTCTC 4 cut(s) 73, 120, 688, 744
BfmI CTRYAG 2 cut(s) 666, 755
BglII AGATCT 1 cut(s) 416
BisI GCNGC 4 cut(s) 19, 459, 645, 666
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 4 cut(s) 20, 460, 646, 667
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 2 cut(s) 357, 392
BmgT120I GGNCC 3 cut(s) 357, 392, 738
BmiI GGNNCC 2 cut(s) 98, 250
BmrFI CCNGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 694
BoxI GACNNNNGTC 1 cut(s) 85
BpmI CTGGAG 1 cut(s) 578
Bpu10I CCTNAGC 1 cut(s) 29
Bpu1102I GCTNAGC 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 647
BsaI GGTCTC 2 cut(s) 120, 744
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 526
Bse1I ACTGG 2 cut(s) 360, 595
BseBI CCWGG 1 cut(s) 302
BseGI GGATG 1 cut(s) 740
BseLI CCNNNNNNNGG 2 cut(s) 401, 526
BseMII CTCAG 2 cut(s) 255, 537
BseNI ACTGG 2 cut(s) 360, 595
BseX3I CGGCCG 1 cut(s) 72
BseXI GCAGC 3 cut(s) 30, 631, 652
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 4 cut(s) 74, 458, 583, 739
BsiEI CGRYCG 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 1 cut(s) 614
BslI CCNNNNNNNGG 2 cut(s) 401, 526
BsmAI GTCTC 4 cut(s) 73, 120, 688, 744
BsmBI CGTCTC 1 cut(s) 73
BsnI GGCC 4 cut(s) 74, 458, 583, 739
Bso31I GGTCTC 2 cut(s) 120, 744
Bsp1286I GDGCHC 1 cut(s) 57
Bsp1407I TGTACA 1 cut(s) 220
Bsp143I GATC 9 cut(s) 96, 103, 108, 185, 248, 283, 313, 416, 530
Bsp1720I GCTNAGC 1 cut(s) 546
BspACI CCGC 3 cut(s) 246, 322, 459
BspANI GGCC 4 cut(s) 74, 458, 583, 739
BspCNI CTCAG 2 cut(s) 256, 538
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 2 cut(s) 98, 250
BspMAI CTGCAG 1 cut(s) 670
BspPI GGATC 7 cut(s) 91, 104, 116, 180, 243, 256, 538
BspTNI GGTCTC 2 cut(s) 120, 744
BsrGI TGTACA 1 cut(s) 220
BsrI ACTGG 2 cut(s) 360, 595
BssMI GATC 9 cut(s) 96, 103, 108, 185, 248, 283, 313, 416, 530
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 2 cut(s) 438, 612
BstAUI TGTACA 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 4 cut(s) 29, 264, 486, 546
BstEII GGTNACC 1 cut(s) 404
BstF5I GGATG 1 cut(s) 740
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 9 cut(s) 99, 106, 111, 188, 251, 286, 316, 419, 533
BstMAI GTCTC 4 cut(s) 73, 120, 688, 744
BstMBI GATC 9 cut(s) 96, 103, 108, 185, 248, 283, 313, 416, 530
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 302
BstPAI GACNNNNGTC 1 cut(s) 85
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 1 cut(s) 300
BstSFI CTRYAG 2 cut(s) 666, 755
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 3 cut(s) 30, 631, 652
BstX2I RGATCY 4 cut(s) 96, 185, 248, 416
BstXI CCANNNNNNTGG 1 cut(s) 602
BstYI RGATCY 4 cut(s) 96, 185, 248, 416
BstZI CGGCCG 1 cut(s) 72
BsuRI GGCC 4 cut(s) 74, 458, 583, 739
BtsCI GGATG 1 cut(s) 740
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 111, 508
Cac8I GCNNGC 1 cut(s) 244
CciI TCATGA 1 cut(s) 364
Cfr13I GGNCC 3 cut(s) 357, 392, 738
CseI GACGC 1 cut(s) 90
Csp6I GTAC 2 cut(s) 221, 268
CviAII CATG 2 cut(s) 287, 365
CviQI GTAC 2 cut(s) 221, 268
DdeI CTNAG 4 cut(s) 29, 264, 486, 546
DpnI GATC 9 cut(s) 98, 105, 110, 187, 250, 285, 315, 418, 532
DpnII GATC 9 cut(s) 96, 103, 108, 185, 248, 283, 313, 416, 530
EaeI YGGCCR 2 cut(s) 72, 456
EagI CGGCCG 1 cut(s) 72
Eam1104I CTCTTC 2 cut(s) 438, 612
EarI CTCTTC 2 cut(s) 438, 612
EciI GGCGGA 2 cut(s) 261, 337
EclXI CGGCCG 1 cut(s) 72
Eco31I GGTCTC 2 cut(s) 120, 744
Eco47I GGWCC 2 cut(s) 357, 392
Eco52I CGGCCG 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 462
Eco91I GGTNACC 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 392
EcoO65I GGTNACC 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 300
Esp3I CGTCTC 1 cut(s) 73
FaeI CATG 2 cut(s) 290, 368
FaiI YATR 7 cut(s) 240, 288, 310, 312, 366, 372, 663
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FatI CATG 2 cut(s) 286, 364
FbaI TGATCA 2 cut(s) 283, 313
Fnu4HI GCNGC 4 cut(s) 19, 459, 645, 666
FokI GGATG 1 cut(s) 747
Fsp4HI GCNGC 4 cut(s) 19, 459, 645, 666
GluI GCNGC 4 cut(s) 19, 459, 645, 666
GsuI CTGGAG 1 cut(s) 578
HaeIII GGCC 4 cut(s) 74, 458, 583, 739
HapII CCGG 1 cut(s) 614
HgaI GACGC 1 cut(s) 90
Hin1II CATG 2 cut(s) 290, 368
HindIII AAGCTT 2 cut(s) 175, 274
HinfI GANTC 2 cut(s) 293, 446
HpaII CCGG 1 cut(s) 614
HphI GGTGA 1 cut(s) 293
Hpy188I TCNGA 4 cut(s) 108, 190, 561, 684
Hpy188III TCNNGA 6 cut(s) 58, 94, 365, 395, 450, 691
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 3 cut(s) 647, 668, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 4 cut(s) 29, 264, 486, 546
HpySE526I ACGT 1 cut(s) 577
Hsp92II CATG 2 cut(s) 290, 368
Ksp22I TGATCA 2 cut(s) 283, 313
Kzo9I GATC 9 cut(s) 96, 103, 108, 185, 248, 283, 313, 416, 530
LmnI GCTCC 1 cut(s) 60
Lsp1109I GCAGC 3 cut(s) 30, 631, 652
LweI GCATC 1 cut(s) 694
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 2 cut(s) 404, 505
MalI GATC 9 cut(s) 98, 105, 110, 187, 250, 285, 315, 418, 532
MboI GATC 9 cut(s) 96, 103, 108, 185, 248, 283, 313, 416, 530
MboII GAAGA 3 cut(s) 455, 581, 629
MflI RGATCY 4 cut(s) 96, 185, 248, 416
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 4 cut(s) 63, 600, 637, 720
MluI ACGCGT 1 cut(s) 82
MlyI GAGTC 1 cut(s) 455
MnlI CCTC 5 cut(s) 340, 534, 613, 624, 690
MseI TTAA 3 cut(s) 41, 599, 726
MspI CCGG 1 cut(s) 614
MspR9I CCNGG 1 cut(s) 302
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeII GATC 9 cut(s) 96, 103, 108, 185, 248, 283, 313, 416, 530
NlaIII CATG 2 cut(s) 290, 368
NlaIV GGNNCC 2 cut(s) 98, 250
NmeAIII GCCGAG 1 cut(s) 100
PagI TCATGA 1 cut(s) 364
PfeI GAWTC 1 cut(s) 293
PkrI GCNGC 4 cut(s) 20, 460, 646, 667
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PpuMI RGGWCCY 1 cut(s) 392
PshAI GACNNNNGTC 1 cut(s) 85
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 300
PspEI GGTNACC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 2 cut(s) 98, 250
PspPI GGNCC 3 cut(s) 357, 392, 738
PspPPI RGGWCCY 1 cut(s) 392
PstI CTGCAG 1 cut(s) 670
PsuI RGATCY 4 cut(s) 96, 185, 248, 416
RsaI GTAC 2 cut(s) 222, 269
RsaNI GTAC 2 cut(s) 221, 268
SaqAI TTAA 3 cut(s) 41, 599, 726
SatI GCNGC 4 cut(s) 19, 459, 645, 666
Sau3AI GATC 9 cut(s) 96, 103, 108, 185, 248, 283, 313, 416, 530
Sau96I GGNCC 3 cut(s) 357, 392, 738
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 1 cut(s) 57
SfaNI GCATC 1 cut(s) 694
SfcI CTRYAG 2 cut(s) 666, 755
SinI GGWCC 2 cut(s) 357, 392
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
Sse9I AATT 4 cut(s) 63, 600, 637, 720
SsiI CCGC 3 cut(s) 246, 322, 459
StyD4I CCNGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 1 cut(s) 580
TasI AATT 4 cut(s) 63, 600, 637, 720
TatI WGTACW 2 cut(s) 220, 267
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 1 cut(s) 293
Tru1I TTAA 3 cut(s) 41, 599, 726
Tru9I TTAA 3 cut(s) 41, 599, 726
TscAI CASTG 2 cut(s) 118, 508
TseI GCWGC 3 cut(s) 18, 644, 665
TspRI CASTG 2 cut(s) 118, 508
VpaK11BI GGWCC 2 cut(s) 357, 392
XapI RAATTY 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.