RchiOBHm_Chr3g0483661

methionine sulfoxide reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007363
Physical Location & Seq
Reverse (-)
29956682 .. 29959531
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44841

Sequence Viewer

Length: 768 bp
ATGGCTATTATTTGCAGCAACATTTCCCTTAGGCGTGTATTAACAACGCTCGTGCTTATGACAATCCTAGCGGCTGAGAAAAGGGTGTGCATCCGGATCCCAGATCAGATCACTGAAACTACAAGGCTAGGGATCCACCCAACGACCAGCCCGCCTTTGAAATCAGCAGTTTTTGCTCTGGGTTGCTTTTGGAGGTCTGAAGCTGTGTTCGGGTGCTTGAATGGGGTTCTTCAGACCACTGTTGGTTATGCTGGAGGATCCAAAACCAATCCTGAGTACAGAAGTTTGGGTGATCATGCCGAGTCTGTGCAGGTGGTATATGATGAAAGGCTAATCAGCTTCAGGCAACTTTTGGAGGTTTTCTGGTCTAGTCATGATTATAGACAAGTATTTGGGCAAGGTCCTGATGTGGGTAACCAATACAGATCTATTGTTTTTACAAATGGAACTGAAGAGTCTAGATTGGCTTCTGTAAGCAAAGGGAGAGAGCAAACTAAGTCAAAGAGCAGCATTGTCACTACTCAAATCCAACCACTTGGAACATTTTATCCTGCTGAGCCTGAACATCAGAAGTTTGAGCTGAAGCGCAGGCCATTCCTTCTTCAACTAATGGGAAACTTGCCTGAAGAGGAACTTGAGAGGTCACATTTGGCAGCGAAACTGAATAGCTATGCCGCAGAGCTTTGCCCTCCAAATATTCAGAAGAAGATTGATGTAAAGATCAACGAGATTATTAAAAAAGGATGGCCAATTTTGAGTGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMSR

Protein Analysis

255

Amino Acids

28.62

Weight (kDa)

8.77

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMSR PF01625 56 - 195 4.2e-44 Peptide methionine sulfoxide reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 301
Acc36I ACCTGC 1 cut(s) 301
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 3 cut(s) 71, 152, 675
AclWI GGATC 6 cut(s) 91, 104, 127, 140, 252, 265
AcoI YGGCCR 1 cut(s) 746
AcuI CTGAAG 6 cut(s) 215, 219, 325, 471, 602, 645
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 3 cut(s) 160, 220, 605
AloI GAACNNNNNNTCC 2 cut(s) 532, 564
AluBI AGCT 5 cut(s) 203, 339, 580, 669, 682
AluI AGCT 5 cut(s) 203, 339, 580, 669, 682
AlwI GGATC 6 cut(s) 91, 104, 127, 140, 252, 265
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 2 cut(s) 590, 746
ApeKI GCWGC 3 cut(s) 15, 507, 653
AspLEI GCGC 1 cut(s) 588
AspS9I GGNCC 1 cut(s) 401
AsuHPI GGTGA 1 cut(s) 302
AvaII GGWCC 1 cut(s) 401
AxyI CCTNAGG 1 cut(s) 29
BalI TGGCCA 1 cut(s) 748
BamHI GGATCC 3 cut(s) 96, 132, 257
BauI CACGAG 1 cut(s) 50
BbvI GCAGC 3 cut(s) 27, 519, 665
BccI CCATC 1 cut(s) 738
BclI TGATCA 1 cut(s) 292
BfaI CTAG 4 cut(s) 68, 128, 369, 459
BfuAI ACCTGC 1 cut(s) 301
BglII AGATCT 1 cut(s) 425
BisI GCNGC 5 cut(s) 16, 72, 508, 654, 675
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 5 cut(s) 17, 73, 509, 655, 676
Bme18I GGWCC 1 cut(s) 401
BmgT120I GGNCC 1 cut(s) 401
BmiI GGNNCC 3 cut(s) 98, 134, 259
BmsI GCATC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 273
Bpu1102I GCTNAGC 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 656
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse21I CCTNAGG 1 cut(s) 29
BseAI TCCGGA 1 cut(s) 93
BseGI GGATG 2 cut(s) 90, 749
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMII CTCAG 3 cut(s) 66, 264, 546
BseXI GCAGC 3 cut(s) 27, 519, 665
BsgI GTGCAG 1 cut(s) 329
BshFI GGCC 2 cut(s) 592, 748
BsiSI CCGG 1 cut(s) 94
BslI CCNNNNNNNGG 1 cut(s) 410
BsnI GGCC 2 cut(s) 592, 748
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 8 cut(s) 96, 103, 108, 132, 257, 292, 425, 720
Bsp1720I GCTNAGC 1 cut(s) 555
BspACI CCGC 3 cut(s) 71, 152, 675
BspANI GGCC 2 cut(s) 592, 748
BspCNI CTCAG 3 cut(s) 67, 265, 547
BspEI TCCGGA 1 cut(s) 93
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 3 cut(s) 98, 134, 259
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 6 cut(s) 91, 104, 127, 140, 252, 265
BssMI GATC 8 cut(s) 96, 103, 108, 132, 257, 292, 425, 720
BssSI CACGAG 1 cut(s) 50
Bst2BI CACGAG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 241
Bst6I CTCTTC 2 cut(s) 447, 621
BstAPI GCANNNNNTGC 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 152, 590
BstDEI CTNAG 5 cut(s) 29, 75, 273, 495, 555
BstEII GGTNACC 1 cut(s) 413
BstF5I GGATG 2 cut(s) 90, 749
BstHHI GCGC 1 cut(s) 588
BstKTI GATC 8 cut(s) 99, 106, 111, 135, 260, 295, 428, 723
BstMBI GATC 8 cut(s) 96, 103, 108, 132, 257, 292, 425, 720
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstPI GGTNACC 1 cut(s) 413
BstV1I GCAGC 3 cut(s) 27, 519, 665
BstX2I RGATCY 4 cut(s) 96, 132, 257, 425
BstXI CCANNNNNNTGG 1 cut(s) 536
BstYI RGATCY 4 cut(s) 96, 132, 257, 425
Bsu36I CCTNAGG 1 cut(s) 29
BsuRI GGCC 2 cut(s) 592, 748
BtsCI GGATG 2 cut(s) 90, 749
BtsIMutI CAGTG 2 cut(s) 111, 237
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 2 cut(s) 152, 590
CciI TCATGA 1 cut(s) 373
CfoI GCGC 1 cut(s) 588
Cfr13I GGNCC 1 cut(s) 401
Csp6I GTAC 1 cut(s) 277
CviAII CATG 2 cut(s) 296, 374
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 5 cut(s) 29, 75, 273, 495, 555
DpnI GATC 8 cut(s) 98, 105, 110, 134, 259, 294, 427, 722
DpnII GATC 8 cut(s) 96, 103, 108, 132, 257, 292, 425, 720
EaeI YGGCCR 1 cut(s) 746
Eam1104I CTCTTC 2 cut(s) 447, 621
EarI CTCTTC 2 cut(s) 447, 621
Eco47I GGWCC 1 cut(s) 401
Eco57I CTGAAG 6 cut(s) 215, 219, 325, 471, 602, 645
Eco81I CCTNAGG 1 cut(s) 29
Eco91I GGTNACC 1 cut(s) 413
EcoO109I RGGNCCY 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 413
FaeI CATG 2 cut(s) 299, 377
FaiI YATR 8 cut(s) 59, 249, 297, 319, 321, 375, 381, 672
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FatI CATG 2 cut(s) 295, 373
FauI CCCGC 1 cut(s) 159
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 5 cut(s) 16, 72, 508, 654, 675
FokI GGATG 2 cut(s) 77, 756
Fsp4HI GCNGC 5 cut(s) 16, 72, 508, 654, 675
FspBI CTAG 4 cut(s) 68, 128, 369, 459
GlaI GCGC 1 cut(s) 587
GluI GCNGC 5 cut(s) 16, 72, 508, 654, 675
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 2 cut(s) 592, 748
HapII CCGG 1 cut(s) 94
HhaI GCGC 1 cut(s) 588
Hin1II CATG 2 cut(s) 299, 377
Hin6I GCGC 1 cut(s) 586
HinP1I GCGC 1 cut(s) 586
HinfI GANTC 2 cut(s) 302, 455
HpaII CCGG 1 cut(s) 94
HphI GGTGA 1 cut(s) 302
Hpy188I TCNGA 5 cut(s) 108, 199, 234, 570, 702
Hpy188III TCNNGA 5 cut(s) 94, 272, 374, 404, 459
HpyAV CCTTC 1 cut(s) 608
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 3 cut(s) 15, 90, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 5 cut(s) 29, 75, 273, 495, 555
Hsp92II CATG 2 cut(s) 299, 377
HspAI GCGC 1 cut(s) 586
Kpn2I TCCGGA 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 8 cut(s) 96, 103, 108, 132, 257, 292, 425, 720
Lsp1109I GCAGC 3 cut(s) 27, 519, 665
LweI GCATC 1 cut(s) 99
MaeI CTAG 4 cut(s) 68, 128, 369, 459
MaeIII GTNAC 4 cut(s) 413, 514, 642, 758
MalI GATC 8 cut(s) 98, 105, 110, 134, 259, 294, 427, 722
MboI GATC 8 cut(s) 96, 103, 108, 132, 257, 292, 425, 720
MboII GAAGA 6 cut(s) 221, 464, 593, 638, 715, 718
MflI RGATCY 4 cut(s) 96, 132, 257, 425
MlsI TGGCCA 1 cut(s) 748
MluCI AATT 1 cut(s) 750
MluNI TGGCCA 1 cut(s) 748
MlyI GAGTC 2 cut(s) 311, 464
MmeI TCCRAC 1 cut(s) 553
MnlI CCTC 6 cut(s) 186, 248, 349, 622, 633, 699
Mox20I TGGCCA 1 cut(s) 748
MroI TCCGGA 1 cut(s) 93
MscI TGGCCA 1 cut(s) 748
MseI TTAA 2 cut(s) 41, 735
Msp20I TGGCCA 1 cut(s) 748
MspI CCGG 1 cut(s) 94
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeII GATC 8 cut(s) 96, 103, 108, 132, 257, 292, 425, 720
NlaIII CATG 2 cut(s) 299, 377
NlaIV GGNNCC 3 cut(s) 98, 134, 259
NmeAIII GCCGAG 1 cut(s) 325
NmuCI GTSAC 3 cut(s) 514, 642, 758
PagI TCATGA 1 cut(s) 373
PaqCI CACCTGC 1 cut(s) 301
PkrI GCNGC 5 cut(s) 17, 73, 509, 655, 676
PleI GAGTC 2 cut(s) 310, 463
PpsI GAGTC 2 cut(s) 310, 463
PpuMI RGGWCCY 1 cut(s) 401
Psp5II RGGWCCY 1 cut(s) 401
PspEI GGTNACC 1 cut(s) 413
PspN4I GGNNCC 3 cut(s) 98, 134, 259
PspPI GGNCC 1 cut(s) 401
PspPPI RGGWCCY 1 cut(s) 401
PsuI RGATCY 4 cut(s) 96, 132, 257, 425
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SaqAI TTAA 2 cut(s) 41, 735
SatI GCNGC 5 cut(s) 16, 72, 508, 654, 675
Sau3AI GATC 8 cut(s) 96, 103, 108, 132, 257, 292, 425, 720
Sau96I GGNCC 1 cut(s) 401
SchI GAGTC 2 cut(s) 311, 464
SfaNI GCATC 1 cut(s) 99
SinI GGWCC 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
Sse9I AATT 1 cut(s) 750
SsiI CCGC 3 cut(s) 71, 152, 675
SspI AATATT 1 cut(s) 697
SspMI CTAG 4 cut(s) 68, 128, 369, 459
TaaI ACNGT 1 cut(s) 241
TasI AATT 1 cut(s) 750
TatI WGTACW 1 cut(s) 276
TauI GCSGC 2 cut(s) 74, 677
Tru1I TTAA 2 cut(s) 41, 735
Tru9I TTAA 2 cut(s) 41, 735
TscAI CASTG 2 cut(s) 118, 244
TseFI GTSAC 3 cut(s) 514, 642, 758
TseI GCWGC 3 cut(s) 15, 507, 653
Tsp45I GTSAC 3 cut(s) 514, 642, 758
TspDTI ATGAA 1 cut(s) 339
TspRI CASTG 2 cut(s) 118, 244
VpaK11BI GGWCC 1 cut(s) 401
XbaI TCTAGA 1 cut(s) 458
XspI CTAG 4 cut(s) 68, 128, 369, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.