pycom04g05080

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004352
Physical Location & Seq
Reverse (-)
4728148 .. 4739008
10861 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05080.1

Sequence Viewer

Length: 4491 bp
ATGGAAGACGCAACGTTAAAAAACAACACCAGTGCCCTTGAAGGTCAAGTACATCTTAAGAAACTTCAGACCGATGAGTTCTTTGAAACTGAAAAATACAGCCCTGGTACTGGTTGGATTTGCACCACGGTTCAACCAAGTCAAGAAATCGTTGAAAGTGCAGGATCTCATCAGGGAGTTCCTGCTGTTGAGTTACCACTCGGCTGGGAATGGTTTGATGATTGGCATTTGGATATGGAATCTGTTAACACTGCTGACGGTTGGGTTTATGCTCCGGATGTTGAGAGTTTAAAATGGCCCGAGTCATATGATCAATTAAGATCTGGAAACTATGGAAGGCAAAGGAGATGGATCAGGAATAGAAAACAAAATGATACACATCAGGAAATATATGTTGGACTTTTGAAACCTGGAGATACGGTATCACTTCCTCTATCTGGGTTAGCACAACCTGGAATGTATGTATTGCGTTTAAGGCCCTCTCTCCGCAATAGTAGTGAATATTCTTGGAGTTCAGTGGTGGATGGGTCTGAACAAACAGAGGACTCTGGTAAGTCAAACGTATGCTCTGGGATAAGTGTGTCTTCTCTCACTGAGTCAGAGGAACTGTTGTATTGCACTCAGATAAGTGGTAGTTCTCCAAGCGGTTCTCATAAGTTGTGGTTTTGTATGAGTGTACAAGCAACAGAAATTTCCAAAGATATTCGTTCTGATCCAATCCAGGATTGGACTCTTGTAATCAAGTCCCCACTATCTATCTCCAATTTTCTTCCGCTTGCAGCTGAATTTTCTGTTTTTGAGATGCAAGATAGTGGTAACTTTTTTGCCTGCTCTAGAGGGGTATTCTTTCCAGGAAAAAGTGTCAATGTTTATAGTGCTGACATAAGAAACCCTTTGTTCTTTTCACTGCTTCCACAAAGAGGGTGGCTGCCTATAAATGAAGCTGTTCTTTTTTCCCATCCTCATGAAGTTCCGCCGAAAACTATAAGTTTGAGAAGTTCGATATCTGGAAGGATTGTTCAAATTGTTCTTGAACAGAATTCTGACAAAGAACGGCCACTGCGAGCTAAGATCATTAGGCTCTATGCTCCTTATTGGTATTCAATTTCTAGGTGTCCCCCTCTCAAACTTAGGCTTTTAGATATCAAAGGAAAGAAACATACACGAAAGGTTGGTAATCCATTTCATTCTAAAAAGGACGATGAAACTATCCTGGAGGAAATAACTGAAGAGGAAATATATGAAGGTCATACAATTGCTTCACCTTTGAAATTTAAATTGTTGGGCCTTGCATTGTCCATTGATCAATCTGGCAAGGAGCAGTTTGGACCTGCCAAAGATCTTTCTCCCCTTGGTGACATGGATGGGTCTTTGGATCTCTATGCTTATGATAGTGAAGGCAATTGTATGCGGATTTTTATTACAACAAAACCATGCCTATATCAATCTGTTCCCACAAAGGTAATTTCTGTCCGACCGTATATGACTTTTACCAATAGACTTGGTCGGGACATATCCATTAAGTTATGCAGTGAGGATGAACCAAAGATTCTTCGAGCATCTGATTCAAGGGTCTCGTTTGTACATCGTGAGTCAGAAGGACATGATAAACTTCAGGTTCGACTAGAAGATACCGACTGGTCATTCCCTGTTCAAATTGTAAAGGAGGATACCATTTATCTTGTTTTGAGAAAGCATGATGGTACTCGGAGATTTTTGAGAACAGAGATTCGGGGCTATGAAGAAGGTTCGCGTTTCATCGTTGTATTCCGCCTCGGATCAACCCGTGGTCCCATCAGAATTGAGAACAGGACAGTCAGCAAAACAATTAGAATTCGCCAGTCTGCTTTTGGTGAGGATGCCTGGATTCGCCTAGCACCTCTTTCAACAACGAATTTTTCTTGGGAAGACCCTTATGGCCAAAAAATCATAGAAGCTGAGGTTGACAGTGCTAGCAATGGGCCTTGGGAACTTGATTTGGAAAGGACTGGGATATGTTATGCAGATGAGGGACTTGGATTACAATTTCATGTTATGGAAGTTGGTGATATCAAGGTAGCTAGGTTCACAGACACAACAACTTCGGGGACAAATTTGGATTTGCAAATTGCTGGAAATTGGGGGCATTCTCATATGCAAAACACCAATCAAAGTAATAGTGCCTCACCTGTTGAGCTTATAATTGAGTTTGGAGTTGTAGGGATCTCTATTGTTGACCATAGACCAAAAGAGGTCTCCTACTTTTATTTTGAGAGAGTCTTCGTATCATATTCGACCGGTTATGATGGTGGTACAACAGCCAGGTTCAAGCTCATATTGGGTCTTCTGCAGCTGGACAACCAGCTCCCTCTAACATTGATGCCAGTGCTGTTGGCTCCAGAAATGAACTCTGACATGCATTATCCAGTGTTCAAAATGACTATTACAGTGCGCAAGGAAAATAGTGATGGCGTACAGGTGTACCCTTATGTTTATATACGGGTAACCGATAAGTGTTGGAGGCTTAATATTCATGAACCAATAATTTGGGCTTTGGTGGATTTCTACAATAATCTACAGTTGGATCGTTTGCCTAAAAGTTCTAGTGTCACAGAAGTTGATCCAGAAATACGCATTGACCTAATAGATGTTTCAGAAGTTAGGCTGAAGGTGTCATTGGAAACAGCACCAGCCCAAAGACCTCATGGGGTTCTAGGAGTGTGGAGCCCCATACTATCAGCTGTCGGAAATGCATTCAAGATTCAGGTGCATTTGAGAAGGGTGATGCACAAAGACAGATTCATGCGGAAGAGTTCCATTGCTTCTGCCATTGGTAACCGTATTTGGAGAGATCTGATCCATAATCCTCTACATTTGATATTTTCAGTCGATGTACTTGGTATGACAAGTTCAACATTGGCTTCTCTTAGTAATGGGTTTGCTGAGCTGTCGACTGATGGACAATTTCTGCAACTGCGTTCAAAGCAGGTGACGTCAAGGAGAATAACTGGGGTGGGTGATGGAATTATGCAAGGTACAGAAGCACTTGTGCAAGGTGTTGCCTTTGGAGTGTCTGGAGTGCTGAAAAAGCCTGTAGAGAGCGCAAGACAGAATGGTGTTTTAGGACTAGTTCATGGGCTTGGGCGTGCTTTTCTAGGAGTGATAGTACAACCAGTCAGTGGGGCATTGGACTTCTTCTCCTTGACTGTTGATGGAATTGGAGCAAGTTGTTCCAAATGCTTAGAGGTTTTCAATAGCAAGACAACCTTTCAGAGAATTAGAAATCCCCGAGCAATCCATGCTGATGCTGTACTTAGAGAGTATTGTGAGAGAGAAGCTGTTGGGCAGATGATTTTATACCTTGCTGAAGCACATCGGCACTTTGGTTGTACTGAACTATTCAAGGAACCATCAAAGTTTGCTTGGTCTGATTATTATGAAGATCATTTTGTTGTGCCTTACCAACGGATTGTTTTAGTTACAAATAAGCGAGTCATGCTGCTTCAGTGTTTGGCTCCAGACAAAATGGATAAGAAACCTTGCAAGATCATGTGGGATGTACCTTGGGAAGAGCTCATGGCTGTGGAGTTGGCAAAAGCAGGCTGCAATCAACCATCTCACTTGATCCTCCACCTCAAAAATTTTAGAAGATCAGAAAATTTTGTCCAGGTGATAAAGTGCAGTGTTGAAGAAATTGAGGGAAATGAACCCCAAGCTGTTAGGATCTGTTCCGTAGTACGGAAGATGTGGAAAGCATCTCAGTCGGACATGAACTGCCTAATTCTGAAGGTTCCATCAAGCCAGCGACATGTGTATTTTTCTGGGTCTGAAGCTGATGGGAGAGAGCACCGTATACCGACCAAGGCAATTACTAGGCTGAGAGACATCACATCATACAATTCTGCATTGGATGGTAGATTTGTTAAACACAGTATTAACTTTTCGAAGATTTGGAGCAGCGAACAAGAATCCAGAAGTCGATGCACGTTGTGCAGAAAACAGGTTCCTGAAGATGGTGTAATATGTAGCATTTGGAGACCCATCTGTCCAGACGGCTATGTCTCGATTGGTGATATTGCTCGCATTGGCAGCCATCCTCCAAACGTTGCTGCTGTTTATCGCAAGATTGATAGATTGTTTGCACTTCCGGTTGGTTATGACCTGGTATGGAGGAACTGCACGGATGACTATGCAGCACCGGTATCGATTTGGCATCCTCGGGCTCCGGAGGGATATGTGTCTCCCGGATGCATTGCTATGGCTGGTTTTAGGGAGCCGGAACTTGACAAAGTGTATTGCGTGTCCGAGTCACTTGCTGAAGAGACGGAATTCGAAGCGCAAAAGGTTTGGTCTGCTCCAGATTCTTACCCGTGGGCGTGTCACATTTATCAAGTCCAAAGCGACGCGCTTCACTTTGTGGCTCTAAGGCAAACCAAGGAAGAATCTGACTGGAAACCTATGAGGATCCTCGACGATCCGCAGCCTCTTTTGCGATCTCTAAAAGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N PH Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

1497

Amino Acids

169.03

Weight (kDa)

6.34

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_VAB PF25036 124 - 640 1.3e-23 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_C PF25037 1086 - 1194 3.1e-14 Intermembrane lipid transfer protein VPS13, C-terminal
Vps62 PF06101 1373 - 1445 1.9e-07 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2180
AarI CACCTGC 1 cut(s) 2956
AasI GACNNNNNNGTC 2 cut(s) 4023, 4037
AatII GACGTC 1 cut(s) 2975
Acc16I TGCGCA 1 cut(s) 2432
Acc36I ACCTGC 2 cut(s) 1339, 2956
AccB7I CCANNNNNTGG 1 cut(s) 3158
AccI GTMKAC 2 cut(s) 2930, 3832
AccII CGCG 2 cut(s) 1753, 4385
AccIII TCCGGA 2 cut(s) 274, 4204
AciI CCGC 8 cut(s) 487, 645, 773, 974, 1411, 1771, 2785, 4457
AclI AACGTT 2 cut(s) 14, 4083
AcoI YGGCCR 2 cut(s) 1055, 1918
AcyI GRCGYC 1 cut(s) 2972
AdeI CACNNNGTG 2 cut(s) 3969, 4396
AfiI CCNNNNNNNGG 6 cut(s) 110, 437, 920, 3158, 3717, 3992
AflII CTTAAG 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 3787
AgeI ACCGGT 2 cut(s) 2276, 4177
AhlI ACTAGT 1 cut(s) 3106
AjuI GAANNNNNNNTTGG 4 cut(s) 378, 410, 2639, 2671
AloI GAACNNNNNNTCC 2 cut(s) 1002, 1034
Alw21I GWGCWC 2 cut(s) 3555, 3828
Alw26I GTCTC 7 cut(s) 1579, 2239, 3855, 4009, 4045, 4224, 4295
Ama87I CYCGRG 3 cut(s) 299, 3267, 4197
Aor13HI TCCGGA 2 cut(s) 274, 4204
AoxI GGCC 6 cut(s) 296, 476, 1055, 1285, 1918, 1961
AsiGI ACCGGT 2 cut(s) 2276, 4177
Asp700I GAANNNNTTC 1 cut(s) 945
AspLEI GCGC 4 cut(s) 2433, 3083, 4318, 4387
AspS9I GGNCC 6 cut(s) 297, 477, 1285, 1328, 1790, 1961
AsuC2I CCSGG 1 cut(s) 4224
AsuII TTCGAA 2 cut(s) 3923, 4311
AsuNHI GCTAGC 1 cut(s) 1952
AvaI CYCGRG 3 cut(s) 299, 3267, 4197
AvaII GGWCC 2 cut(s) 1328, 1790
BaeGI GKGCMC 1 cut(s) 37
BaeI ACNNNNGTAYC 2 cut(s) 2444, 2477
BalI TGGCCA 1 cut(s) 1920
BamHI GGATCC 1 cut(s) 4443
BanII GRGCYC 3 cut(s) 2708, 3555, 4204
BarI GAAGNNNNNNTAC 2 cut(s) 33, 65
BbsI GAAGAC 5 cut(s) 12, 576, 1914, 2251, 2315
Bbv12I GWGCWC 2 cut(s) 3555, 3828
BbvCI CCTCAGC 1 cut(s) 1938
BceAI ACGGC 2 cut(s) 1070, 4048
BcgI CGANNNNNNTGC 6 cut(s) 3723, 3757, 4164, 4198, 4274, 4308
BciVI GTATCC 1 cut(s) 1663
BclI TGATCA 2 cut(s) 310, 1303
BcnI CCSGG 1 cut(s) 4224
BcoDI GTCTC 7 cut(s) 1579, 2239, 3855, 4009, 4045, 4224, 4295
BcuI ACTAGT 1 cut(s) 3106
BfmI CTRYAG 3 cut(s) 2327, 2555, 3072
BfrI CTTAAG 1 cut(s) 56
BfuAI ACCTGC 2 cut(s) 1339, 2956
BfuI GTATCC 1 cut(s) 1663
BglII AGATCT 3 cut(s) 320, 1339, 2830
BlpI GCTNAGC 1 cut(s) 2922
Bme18I GGWCC 2 cut(s) 1328, 1790
BmeT110I CYCGRG 3 cut(s) 299, 3267, 4197
BmgT120I GGNCC 6 cut(s) 297, 477, 1285, 1328, 1790, 1961
BmrI ACTGGG 2 cut(s) 1998, 2997
BmtI GCTAGC 1 cut(s) 1956
BmuI ACTGGG 2 cut(s) 1998, 2997
BpiI GAAGAC 5 cut(s) 12, 576, 1914, 2251, 2315
BpmI CTGGAG 6 cut(s) 432, 1235, 2361, 3075, 3480, 4320
Bpu10I CCTNAGC 1 cut(s) 1938
Bpu1102I GCTNAGC 1 cut(s) 2922
Bpu14I TTCGAA 2 cut(s) 3923, 4311
BpuMI CCSGG 1 cut(s) 4224
Bsa29I ATCGAT 1 cut(s) 4184
BsaBI GATNNNNATC 1 cut(s) 378
BsaHI GRCGYC 1 cut(s) 2972
BsaI GGTCTC 3 cut(s) 1579, 2239, 4009
BsaWI WCCGGW 5 cut(s) 274, 2276, 4126, 4177, 4204
BsaXI ACNNNNNCTCC 6 cut(s) 405, 435, 3129, 3159, 3161, 3191
Bsc4I CCNNNNNNNGG 6 cut(s) 110, 437, 920, 3158, 3717, 3992
Bse118I RCCGGY 2 cut(s) 2276, 4177
Bse3DI GCAATG 3 cut(s) 1963, 2796, 4230
Bse8I GATNNNNATC 1 cut(s) 378
BseAI TCCGGA 2 cut(s) 274, 4204
BseCI ATCGAT 1 cut(s) 4184
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 6 cut(s) 110, 437, 920, 3158, 3717, 3992
BseMI GCAATG 3 cut(s) 1963, 2796, 4230
BseMII CTCAG 6 cut(s) 585, 635, 1929, 2913, 3752, 3848
BseSI GKGCMC 1 cut(s) 37
BseYI CCCAGC 1 cut(s) 204
BsgI GTGCAG 4 cut(s) 180, 3679, 3991, 4141
Bsh1236I CGCG 2 cut(s) 1753, 4385
Bsh1285I CGRYCG 2 cut(s) 1478, 2277
BshFI GGCC 6 cut(s) 298, 478, 1057, 1287, 1920, 1963
BshTI ACCGGT 2 cut(s) 2276, 4177
BshVI ATCGAT 1 cut(s) 4184
BsiEI CGRYCG 2 cut(s) 1478, 2277
BsiHKAI GWGCWC 2 cut(s) 3555, 3828
BsiHKCI CYCGRG 3 cut(s) 299, 3267, 4197
BsiSI CCGG 7 cut(s) 275, 2277, 4127, 4178, 4205, 4224, 4256
BslFI GGGAC 6 cut(s) 730, 1101, 1523, 1776, 2025, 2101
BslI CCNNNNNNNGG 6 cut(s) 110, 437, 920, 3158, 3717, 3992
BsmAI GTCTC 7 cut(s) 1579, 2239, 3855, 4009, 4045, 4224, 4295
BsmBI CGTCTC 1 cut(s) 4295
BsmFI GGGAC 6 cut(s) 730, 1101, 1523, 1776, 2025, 2101
BsmI GAATGC 2 cut(s) 2125, 2732
BsnI GGCC 6 cut(s) 298, 478, 1057, 1287, 1920, 1963
Bso31I GGTCTC 3 cut(s) 1579, 2239, 4009
BsoBI CYCGRG 3 cut(s) 299, 3267, 4197
Bsp119I TTCGAA 2 cut(s) 3923, 4311
Bsp1286I GDGCHC 5 cut(s) 37, 2708, 3555, 3828, 4204
Bsp13I TCCGGA 2 cut(s) 274, 4204
Bsp1407I TGTACA 2 cut(s) 676, 1582
Bsp1720I GCTNAGC 1 cut(s) 2922
BspACI CCGC 8 cut(s) 487, 645, 773, 974, 1411, 1771, 2785, 4457
BspANI GGCC 6 cut(s) 298, 478, 1057, 1287, 1920, 1963
BspCNI CTCAG 6 cut(s) 586, 634, 1930, 2914, 3751, 3849
BspDI ATCGAT 1 cut(s) 4184
BspEI TCCGGA 2 cut(s) 274, 4204
BspFNI CGCG 2 cut(s) 1753, 4385
BspHI TCATGA 2 cut(s) 964, 2512
BspMAI CTGCAG 1 cut(s) 2331
BspMI ACCTGC 2 cut(s) 1339, 2956
BspOI GCTAGC 1 cut(s) 1956
BspQI GCTCTTC 1 cut(s) 3543
BspT104I TTCGAA 2 cut(s) 3923, 4311
BspTI CTTAAG 1 cut(s) 56
BspTNI GGTCTC 3 cut(s) 1579, 2239, 4009
BsrDI GCAATG 3 cut(s) 1963, 2796, 4230
BsrFI RCCGGY 2 cut(s) 2276, 4177
BsrGI TGTACA 2 cut(s) 676, 1582
BssAI RCCGGY 2 cut(s) 2276, 4177
BssNAI GTATAC 1 cut(s) 3833
BssNI GRCGYC 1 cut(s) 2972
BssT1I CCWWGG 5 cut(s) 1351, 1964, 3542, 3840, 4413
Bst1107I GTATAC 1 cut(s) 3833
Bst6I CTCTTC 4 cut(s) 1224, 2783, 3543, 4293
BstACI GRCGYC 1 cut(s) 2972
BstAFI CTTAAG 1 cut(s) 56
BstAPI GCANNNNNTGC 2 cut(s) 3278, 3969
BstAUI TGTACA 2 cut(s) 676, 1582
BstBI TTCGAA 2 cut(s) 3923, 4311
BstC8I GCNNGC 8 cut(s) 777, 829, 1065, 1954, 3126, 3580, 3782, 4060
BstDSI CCRYGG 3 cut(s) 126, 1786, 4349
BstEII GGTNACC 2 cut(s) 2482, 2813
BstFNI CGCG 2 cut(s) 1753, 4385
BstHHI GCGC 4 cut(s) 2433, 3083, 4318, 4387
BstMAI GTCTC 7 cut(s) 1579, 2239, 3855, 4009, 4045, 4224, 4295
BstMCI CGRYCG 2 cut(s) 1478, 2277
BstMWI GCNNNNNNNGC 8 cut(s) 475, 2962, 3067, 3278, 3475, 3969, 4068, 4468
BstNSI RCATGY 2 cut(s) 2398, 3791
BstPI GGTNACC 2 cut(s) 2482, 2813
BstSFI CTRYAG 3 cut(s) 2327, 2555, 3072
BstSLI GKGCMC 1 cut(s) 37
BstUI CGCG 2 cut(s) 1753, 4385
BstV2I GAAGAC 5 cut(s) 12, 576, 1914, 2251, 2315
BstX2I RGATCY 8 cut(s) 164, 320, 1339, 1375, 2202, 2830, 3702, 4443
BstXI CCANNNNNNTGG 2 cut(s) 204, 2526
BstYI RGATCY 8 cut(s) 164, 320, 1339, 1375, 2202, 2830, 3702, 4443
BstZ17I GTATAC 1 cut(s) 3833
Bsu15I ATCGAT 1 cut(s) 4184
BsuI GTATCC 1 cut(s) 1663
BsuRI GGCC 6 cut(s) 298, 478, 1057, 1287, 1920, 1963
BsuTUI ATCGAT 1 cut(s) 4184
BtgI CCRYGG 3 cut(s) 126, 1786, 4349
BtsI GCAGTG 5 cut(s) 249, 905, 1058, 1537, 3667
BveI ACCTGC 2 cut(s) 1339, 2956
Cac8I GCNNGC 8 cut(s) 777, 829, 1065, 1954, 3126, 3580, 3782, 4060
CciI TCATGA 2 cut(s) 964, 2512
CfoI GCGC 4 cut(s) 2433, 3083, 4318, 4387
Cfr10I RCCGGY 2 cut(s) 2276, 4177
Cfr13I GGNCC 6 cut(s) 297, 477, 1285, 1328, 1790, 1961
ClaI ATCGAT 1 cut(s) 4184
CseI GACGC 2 cut(s) 17, 4391
CsiI ACCWGGT 1 cut(s) 4140
CspAI ACCGGT 2 cut(s) 2276, 4177
CspCI CAANNNNNGTGG 2 cut(s) 905, 940
DraI TTTAAA 2 cut(s) 291, 1276
DraIII CACNNNGTG 2 cut(s) 3969, 4396
DrdI GACNNNNNNGTC 2 cut(s) 4023, 4037
DseDI GACNNNNNNGTC 2 cut(s) 4023, 4037
EaeI YGGCCR 2 cut(s) 1055, 1918
Eam1104I CTCTTC 4 cut(s) 1224, 2783, 3543, 4293
EarI CTCTTC 4 cut(s) 1224, 2783, 3543, 4293
EciI GGCGGA 2 cut(s) 963, 1760
Ecl136II GAGCTC 1 cut(s) 3553
Eco130I CCWWGG 5 cut(s) 1351, 1964, 3542, 3840, 4413
Eco24I GRGCYC 3 cut(s) 2708, 3555, 4204
Eco31I GGTCTC 3 cut(s) 1579, 2239, 4009
Eco32I GATATC 3 cut(s) 1005, 1144, 2050
Eco47I GGWCC 2 cut(s) 1328, 1790
Eco53kI GAGCTC 1 cut(s) 3553
Eco88I CYCGRG 3 cut(s) 299, 3267, 4197
Eco91I GGTNACC 2 cut(s) 2482, 2813
EcoICRI GAGCTC 1 cut(s) 3553
EcoO109I RGGNCCY 1 cut(s) 477
EcoO65I GGTNACC 2 cut(s) 2482, 2813
EcoRI GAATTC 3 cut(s) 1039, 1833, 4307
EcoRV GATATC 3 cut(s) 1005, 1144, 2050
EcoT14I CCWWGG 5 cut(s) 1351, 1964, 3542, 3840, 4413
EcoT22I ATGCAT 3 cut(s) 2400, 2734, 4232
EcoT38I GRGCYC 3 cut(s) 2708, 3555, 4204
ErhI CCWWGG 5 cut(s) 1351, 1964, 3542, 3840, 4413
Esp3I CGTCTC 1 cut(s) 4295
FaqI GGGAC 6 cut(s) 730, 1101, 1523, 1776, 2025, 2101
FauNDI CATATG 2 cut(s) 307, 2133
FbaI TGATCA 2 cut(s) 310, 1303
FblI GTMKAC 2 cut(s) 2930, 3832
FriOI GRGCYC 3 cut(s) 2708, 3555, 4204
FspI TGCGCA 1 cut(s) 2432
GlaI GCGC 4 cut(s) 2432, 3082, 4317, 4386
GsaI CCCAGC 1 cut(s) 208
GsuI CTGGAG 6 cut(s) 432, 1235, 2361, 3075, 3480, 4320
HaeIII GGCC 6 cut(s) 298, 478, 1057, 1287, 1920, 1963
HapII CCGG 7 cut(s) 275, 2277, 4127, 4178, 4205, 4224, 4256
HgaI GACGC 2 cut(s) 17, 4391
HhaI GCGC 4 cut(s) 2433, 3083, 4318, 4387
Hin1I GRCGYC 1 cut(s) 2972
Hin6I GCGC 4 cut(s) 2431, 3081, 4316, 4385
HinP1I GCGC 4 cut(s) 2431, 3081, 4316, 4385
HincII GTYRAC 4 cut(s) 247, 1945, 2215, 2931
HindII GTYRAC 4 cut(s) 247, 1945, 2215, 2931
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 7 cut(s) 275, 2277, 4127, 4178, 4205, 4224, 4256
Hpy166II GTNNAC 8 cut(s) 247, 677, 1945, 2067, 2215, 2461, 2931, 3833
Hpy8I GTNNAC 8 cut(s) 247, 677, 1945, 2067, 2215, 2461, 2931, 3833
Hpy99I CGWCG 2 cut(s) 4385, 4454
HpyCH4IV ACGT 5 cut(s) 14, 561, 2972, 3965, 4083
HpyF10VI GCNNNNNNNGC 8 cut(s) 475, 2962, 3067, 3278, 3475, 3969, 4068, 4468
HpySE526I ACGT 5 cut(s) 14, 561, 2972, 3965, 4083
Hsp92I GRCGYC 1 cut(s) 2972
HspAI GCGC 4 cut(s) 2431, 3081, 4316, 4385
Kpn2I TCCGGA 2 cut(s) 274, 4204
Ksp22I TGATCA 2 cut(s) 310, 1303
KspAI GTTAAC 1 cut(s) 247
LguI GCTCTTC 1 cut(s) 3543
MabI ACCWGGT 1 cut(s) 4140
MaeII ACGT 5 cut(s) 14, 561, 2972, 3965, 4083
MfeI CAATTG 2 cut(s) 1254, 1402
MflI RGATCY 8 cut(s) 164, 320, 1339, 1375, 2202, 2830, 3702, 4443
MhlI GDGCHC 5 cut(s) 37, 2708, 3555, 3828, 4204
MlsI TGGCCA 1 cut(s) 1920
MluNI TGGCCA 1 cut(s) 1920
MlyI GAGTC 8 cut(s) 311, 539, 605, 724, 1601, 2265, 3480, 4295
MmeI TCCRAC 7 cut(s) 95, 376, 1498, 2477, 2541, 2704, 3723
Mox20I TGGCCA 1 cut(s) 1920
Mph1103I ATGCAT 3 cut(s) 2400, 2734, 4232
MroI TCCGGA 2 cut(s) 274, 4204
MroXI GAANNNNTTC 1 cut(s) 945
MscI TGGCCA 1 cut(s) 1920
MslI CAYNNNNRTG 4 cut(s) 963, 3282, 3560, 4235
Msp20I TGGCCA 1 cut(s) 1920
MspA1I CMGCKG 3 cut(s) 782, 2332, 2720
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 7 cut(s) 275, 2277, 4127, 4178, 4205, 4224, 4256
MunI CAATTG 2 cut(s) 1254, 1402
Mva1269I GAATGC 2 cut(s) 2125, 2732
MvnI CGCG 2 cut(s) 1753, 4385
MwoI GCNNNNNNNGC 8 cut(s) 475, 2962, 3067, 3278, 3475, 3969, 4068, 4468
NciI CCSGG 1 cut(s) 4224
NdeI CATATG 2 cut(s) 307, 2133
NheI GCTAGC 1 cut(s) 1952
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 5 cut(s) 1355, 2587, 2968, 4287, 4358
NsbI TGCGCA 1 cut(s) 2432
NsiI ATGCAT 3 cut(s) 2400, 2734, 4232
NspI RCATGY 2 cut(s) 2398, 3791
NspV TTCGAA 2 cut(s) 3923, 4311
PagI TCATGA 2 cut(s) 964, 2512
PaqCI CACCTGC 1 cut(s) 2956
PciI ACATGT 1 cut(s) 3787
PciSI GCTCTTC 1 cut(s) 3543
PcsI WCGNNNNNNNCGW 1 cut(s) 1060
PctI GAATGC 2 cut(s) 2125, 2732
PdmI GAANNNNTTC 1 cut(s) 945
PflFI GACNNNGTC 1 cut(s) 1503
PflMI CCANNNNNTGG 1 cut(s) 3158
PfoI TCCNGGA 4 cut(s) 720, 850, 1212, 4222
PinAI ACCGGT 2 cut(s) 2276, 4177
PleI GAGTC 8 cut(s) 310, 539, 604, 724, 1600, 2264, 3479, 4294
PpsI GAGTC 8 cut(s) 310, 539, 604, 724, 1600, 2264, 3479, 4294
PscI ACATGT 1 cut(s) 3787
PsiI TTATAA 1 cut(s) 2180
Psp124BI GAGCTC 1 cut(s) 3555
Psp1406I AACGTT 2 cut(s) 14, 4083
PspEI GGTNACC 2 cut(s) 2482, 2813
PspFI CCCAGC 1 cut(s) 204
PspPI GGNCC 6 cut(s) 297, 477, 1285, 1328, 1790, 1961
PsrI GAACNNNNNNTAC 2 cut(s) 2872, 2904
PstI CTGCAG 1 cut(s) 2331
PsuI RGATCY 8 cut(s) 164, 320, 1339, 1375, 2202, 2830, 3702, 4443
PsyI GACNNNGTC 1 cut(s) 1503
PvuII CAGCTG 3 cut(s) 782, 2332, 2720
RseI CAYNNNNRTG 4 cut(s) 963, 3282, 3560, 4235
SacI GAGCTC 1 cut(s) 3555
SalI GTCGAC 1 cut(s) 2929
SapI GCTCTTC 1 cut(s) 3543
Sau96I GGNCC 6 cut(s) 297, 477, 1285, 1328, 1790, 1961
SchI GAGTC 8 cut(s) 311, 539, 605, 724, 1601, 2265, 3480, 4295
SduI GDGCHC 5 cut(s) 37, 2708, 3555, 3828, 4204
SexAI ACCWGGT 1 cut(s) 4140
SfcI CTRYAG 3 cut(s) 2327, 2555, 3072
SfuI TTCGAA 2 cut(s) 3923, 4311
SinI GGWCC 2 cut(s) 1328, 1790
SmiI ATTTAAAT 1 cut(s) 1276
SmiMI CAYNNNNRTG 4 cut(s) 963, 3282, 3560, 4235
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SpeI ACTAGT 1 cut(s) 3106
SsiI CCGC 8 cut(s) 487, 645, 773, 974, 1411, 1771, 2785, 4457
SspI AATATT 2 cut(s) 503, 2509
SstI GAGCTC 1 cut(s) 3555
StyI CCWWGG 5 cut(s) 1351, 1964, 3542, 3840, 4413
SwaI ATTTAAAT 1 cut(s) 1276
TaiI ACGT 5 cut(s) 17, 564, 2975, 3968, 4086
TaqII GACCGA 1 cut(s) 86
TatI WGTACW 7 cut(s) 49, 676, 1582, 2872, 3145, 3289, 3368
TseFI GTSAC 5 cut(s) 1355, 2587, 2968, 4287, 4358
Tsp45I GTSAC 5 cut(s) 1355, 2587, 2968, 4287, 4358
TspGWI ACGGA 5 cut(s) 3460, 3700, 3733, 4175, 4319
Tth111I GACNNNGTC 1 cut(s) 1503
Van91I CCANNNNNTGG 1 cut(s) 3158
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 2 cut(s) 1328, 1790
XbaI TCTAGA 1 cut(s) 833
XceI RCATGY 2 cut(s) 2398, 3791
XcmI CCANNNNNNNNNTGG 4 cut(s) 723, 921, 1847, 2681
XmiI GTMKAC 2 cut(s) 2930, 3832
XmnI GAANNNNTTC 1 cut(s) 945
ZraI GACGTC 1 cut(s) 2973
Zsp2I ATGCAT 3 cut(s) 2400, 2734, 4232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.