pycom04g05230

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0017299
Physical Location & Seq
Forward (+)
5029689 .. 5030699
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05230.3

Sequence Viewer

Length: 738 bp
ATGGATGAAATTGTTAGGAGCTCACATTTTGTACTTGATGACAATGATCGAGTTTTCACCTTCTCTCGTTCTCTCTCGCAGAAAAAATTCGGTGTAGAAGATATCTTCAACGAGAATCTCTATTATACCAACAAGACCCTCAATTGGGGAATGAATGCCAAGCTATCATCTGCTCTGTACCTTCAAGTTAGCACCATCAGCCAGGCTCTAATTTTTGTCACACGCTCCCGAGGATGGTCATTCCTGGAAGTACCTGGTCTGCTTCTTCTTAGTGCTTTCGTAATTGCTCAACTGATTGCTACATTAATATCTGCTGTAGCAACATGGGAATTTGCTAGAATTCAAGCAATCGGCTGGAAATGGTGTGGTATCGTATGGATATACAACATTCTAGTCTACATGCTACTTGATCCTGTCAAGATTTTTGTTCGATATGCACTCAGTGGGAGGGCTTGGAGTTTGGTATTGAACAAAAGAACGGCTTTCAACACCCAAAAGGACTTTGGTAAAGAGTTCCGCGAGGCTGCATGGGCGGCAGAACAGCGGACGGTCCATGGCCTCCCATCTGTGGAGGCAAAGAACATTCCTGAGAGGCACACATTCAGGGATGTTAGCATCATGACTGAAGAAGCCAGGAGACGTGCAGAGATTGCAAGGTTGGTTTTCCTTCTTAAGTTGGACGTTGATCACAAATCAATTGTATACAAGTTTACGAAAAGGAAAAAAACGATAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

246

Amino Acids

28.35

Weight (kDa)

9.81

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 396, 702
AccII CGCG 1 cut(s) 519
AciI CCGC 3 cut(s) 517, 533, 544
AclWI GGATC 1 cut(s) 404
AcsI RAATTY 3 cut(s) 86, 329, 339
AcuI CTGAAG 1 cut(s) 645
AdeI CACNNNGTG 1 cut(s) 443
AfaI GTAC 3 cut(s) 33, 179, 252
AfiI CCNNNNNNNGG 4 cut(s) 144, 145, 234, 568
AflII CTTAAG 1 cut(s) 671
AgsI TTSAA 5 cut(s) 109, 185, 344, 469, 487
AjiI CACGTC 1 cut(s) 641
AjnI CCWGG 4 cut(s) 201, 243, 253, 632
AluBI AGCT 2 cut(s) 21, 163
AluI AGCT 2 cut(s) 21, 163
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 631
AlwI GGATC 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 228
AoxI GGCC 1 cut(s) 556
ApeKI GCWGC 1 cut(s) 524
ApoI RAATTY 3 cut(s) 86, 329, 339
AseI ATTAAT 1 cut(s) 305
Asp700I GAANNNNTTC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 550
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 1 cut(s) 228
AvaII GGWCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 511
BccI CCATC 3 cut(s) 203, 228, 571
BceAI ACGGC 1 cut(s) 495
BciT130I CCWGG 4 cut(s) 203, 245, 255, 634
BclI TGATCA 1 cut(s) 685
BcoDI GTCTC 1 cut(s) 631
BfaI CTAG 2 cut(s) 336, 392
BfmI CTRYAG 1 cut(s) 315
BfrI CTTAAG 1 cut(s) 671
BisI GCNGC 2 cut(s) 525, 534
BlsI GCNGC 2 cut(s) 526, 535
Bme1390I CCNGG 4 cut(s) 203, 245, 255, 634
Bme18I GGWCC 1 cut(s) 550
BmeT110I CYCGRG 1 cut(s) 228
BmgBI CACGTC 1 cut(s) 641
BmgT120I GGNCC 1 cut(s) 550
BmrFI CCNGG 4 cut(s) 203, 245, 255, 634
BmsI GCATC 1 cut(s) 624
BsaJI CCNNGG 2 cut(s) 229, 553
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 145, 234, 568
BseBI CCWGG 4 cut(s) 203, 245, 255, 634
BseDI CCNNGG 2 cut(s) 229, 553
BseGI GGATG 3 cut(s) 10, 239, 613
BseLI CCNNNNNNNGG 4 cut(s) 144, 145, 234, 568
BseMII CTCAG 2 cut(s) 454, 579
BseXI GCAGC 1 cut(s) 511
BsgI GTGCAG 1 cut(s) 663
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 1 cut(s) 558
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 228
BslI CCNNNNNNNGG 4 cut(s) 144, 145, 234, 568
BsmAI GTCTC 1 cut(s) 631
BsmBI CGTCTC 1 cut(s) 631
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 1 cut(s) 558
BsoBI CYCGRG 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 3 cut(s) 46, 409, 685
Bsp19I CCATGG 1 cut(s) 553
BspACI CCGC 3 cut(s) 517, 533, 544
BspANI GGCC 1 cut(s) 558
BspCNI CTCAG 2 cut(s) 453, 580
BspFNI CGCG 1 cut(s) 519
BspHI TCATGA 1 cut(s) 618
BspPI GGATC 1 cut(s) 404
BspTI CTTAAG 1 cut(s) 671
BssECI CCNNGG 2 cut(s) 229, 553
BssMI GATC 3 cut(s) 46, 409, 685
BssNAI GTATAC 1 cut(s) 703
BssT1I CCWWGG 1 cut(s) 553
Bst1107I GTATAC 1 cut(s) 703
Bst2UI CCWGG 4 cut(s) 203, 245, 255, 634
Bst4CI ACNGT 1 cut(s) 550
BstAFI CTTAAG 1 cut(s) 671
BstAPI GCANNNNNTGC 1 cut(s) 650
BstDEI CTNAG 3 cut(s) 269, 440, 588
BstDSI CCRYGG 1 cut(s) 553
BstF5I GGATG 3 cut(s) 10, 239, 613
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 3 cut(s) 49, 412, 688
BstMAI GTCTC 1 cut(s) 631
BstMBI GATC 3 cut(s) 46, 409, 685
BstMWI GCNNNNNNNGC 4 cut(s) 198, 530, 533, 650
BstNI CCWGG 4 cut(s) 203, 245, 255, 634
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 4 cut(s) 201, 243, 253, 632
BstSFI CTRYAG 1 cut(s) 315
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 1 cut(s) 511
BstZ17I GTATAC 1 cut(s) 703
BsuRI GGCC 1 cut(s) 558
BtgI CCRYGG 1 cut(s) 553
BtrI CACGTC 1 cut(s) 641
BtsCI GGATG 3 cut(s) 10, 239, 613
BtsIMutI CAGTG 1 cut(s) 448
CciI TCATGA 1 cut(s) 618
Cfr13I GGNCC 1 cut(s) 550
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 3 cut(s) 32, 178, 251
CviAII CATG 5 cut(s) 324, 400, 528, 554, 619
CviQI GTAC 3 cut(s) 32, 178, 251
DdeI CTNAG 3 cut(s) 269, 440, 588
DpnI GATC 3 cut(s) 48, 411, 687
DpnII GATC 3 cut(s) 46, 409, 685
DraIII CACNNNGTG 1 cut(s) 443
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 553
Eco24I GRGCYC 1 cut(s) 23
Eco32I GATATC 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 550
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 645
Eco88I CYCGRG 1 cut(s) 228
EcoICRI GAGCTC 1 cut(s) 21
EcoRI GAATTC 1 cut(s) 339
EcoRII CCWGG 4 cut(s) 201, 243, 253, 632
EcoRV GATATC 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 553
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 553
Esp3I CGTCTC 1 cut(s) 631
FaeI CATG 5 cut(s) 327, 403, 531, 557, 622
FalI AAGNNNNNCTT 2 cut(s) 466, 498
FatI CATG 5 cut(s) 323, 399, 527, 553, 618
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 2 cut(s) 396, 702
Fnu4HI GCNGC 2 cut(s) 525, 534
FokI GGATG 3 cut(s) 17, 246, 620
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 525, 534
FspBI CTAG 2 cut(s) 336, 392
GluI GCNGC 2 cut(s) 525, 534
HaeIII GGCC 1 cut(s) 558
Hin1II CATG 5 cut(s) 327, 403, 531, 557, 622
HinfI GANTC 1 cut(s) 115
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 3 cut(s) 397, 703, 711
Hpy188III TCNNGA 4 cut(s) 228, 418, 587, 619
Hpy8I GTNNAC 3 cut(s) 397, 703, 711
HpyAV CCTTC 3 cut(s) 70, 191, 677
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 2 cut(s) 640, 681
HpyCH4V TGCA 4 cut(s) 437, 527, 644, 653
HpyF10VI GCNNNNNNNGC 4 cut(s) 198, 530, 533, 650
HpyF3I CTNAG 3 cut(s) 269, 440, 588
HpySE526I ACGT 2 cut(s) 640, 681
Hsp92II CATG 5 cut(s) 327, 403, 531, 557, 622
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 3 cut(s) 46, 409, 685
LmnI GCTCC 2 cut(s) 18, 230
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 1 cut(s) 624
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 2 cut(s) 336, 392
MaeII ACGT 2 cut(s) 640, 681
MaeIII GTNAC 1 cut(s) 217
MalI GATC 3 cut(s) 48, 411, 687
MboI GATC 3 cut(s) 46, 409, 685
MboII GAAGA 4 cut(s) 97, 110, 257, 638
MfeI CAATTG 2 cut(s) 142, 696
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 8 cut(s) 9, 86, 142, 210, 282, 329, 339, 696
MmeI TCCRAC 1 cut(s) 657
MnlI CCTC 7 cut(s) 149, 224, 441, 514, 565, 569, 585
MroXI GAANNNNTTC 1 cut(s) 86
MseI TTAA 2 cut(s) 305, 672
MspA1I CMGCKG 1 cut(s) 544
MspCI CTTAAG 1 cut(s) 671
MspR9I CCNGG 4 cut(s) 203, 245, 255, 634
MunI CAATTG 2 cut(s) 142, 696
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 4 cut(s) 203, 245, 255, 634
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 4 cut(s) 198, 530, 533, 650
NcoI CCATGG 1 cut(s) 553
NdeII GATC 3 cut(s) 46, 409, 685
NlaIII CATG 5 cut(s) 327, 403, 531, 557, 622
NmuCI GTSAC 1 cut(s) 217
NspI RCATGY 1 cut(s) 403
PagI TCATGA 1 cut(s) 618
PctI GAATGC 1 cut(s) 160
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 115
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 2 cut(s) 526, 535
PshBI ATTAAT 1 cut(s) 305
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 4 cut(s) 201, 243, 253, 632
PspGI CCWGG 4 cut(s) 201, 243, 253, 632
PspPI GGNCC 1 cut(s) 550
RsaI GTAC 3 cut(s) 33, 179, 252
RsaNI GTAC 3 cut(s) 32, 178, 251
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 305, 672
SatI GCNGC 2 cut(s) 525, 534
Sau3AI GATC 3 cut(s) 46, 409, 685
Sau96I GGNCC 1 cut(s) 550
ScrFI CCNGG 4 cut(s) 203, 245, 255, 634
SduI GDGCHC 1 cut(s) 23
SetI ASST 8 cut(s) 23, 62, 165, 183, 256, 643, 659, 684
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 1 cut(s) 624
SfcI CTRYAG 1 cut(s) 315
SinI GGWCC 1 cut(s) 550
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
Sse9I AATT 8 cut(s) 9, 86, 142, 210, 282, 329, 339, 696
SsiI CCGC 3 cut(s) 517, 533, 544
SspMI CTAG 2 cut(s) 336, 392
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 4 cut(s) 201, 243, 253, 632
StyI CCWWGG 1 cut(s) 553
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 2 cut(s) 643, 684
TaqI TCGA 2 cut(s) 49, 430
TasI AATT 8 cut(s) 9, 86, 142, 210, 282, 329, 339, 696
TatI WGTACW 1 cut(s) 31
TauI GCSGC 1 cut(s) 536
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 2 cut(s) 305, 672
Tru9I TTAA 2 cut(s) 305, 672
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 21, 167
TspRI CASTG 1 cut(s) 448
Vha464I CTTAAG 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 550
VspI ATTAAT 1 cut(s) 305
XapI RAATTY 3 cut(s) 86, 329, 339
XceI RCATGY 1 cut(s) 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmiI GTMKAC 2 cut(s) 396, 702
XmnI GAANNNNTTC 1 cut(s) 86
XspI CTAG 2 cut(s) 336, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.