pycom04g06030

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013870
Physical Location & Seq
Forward (+)
5914536 .. 5917355
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1257 bp
ATGTACGTACAATGTACGAAGAACGTATATGATTCACGGATCCTAAACGGTTTACTGTGCGAACCAACCACCAGTCCACAGCTAAGAAAAAATAACAATAAAGAAGTAAAACAACGACAAGATGATGCTCTCTCTCCCTCTCCTCATATTTTCTCATACATCAGACACCGCAATTCTCTACCCCTCCTTTTCTCTCGGGAAACAAACAGCCAAACACAATCCTCCCACAGCTTGTATTGTACGCGGCCCCTCCCCTCTACAAATCATCACCAAGTTTCATACTTTTTGCCACCAATAAATCCCCCATGTCCACTCCCACCATACCCACTTCTCTCTCCTCAATTCTTCAAGCTTTGTCTCTCTAGAATTGCTGTGTTCTGTTTGACTATGGGTGCCAGTTTCTCCTCTGTCTCCAAATCGGAAAACCCAGTTGCAGTCCTATCTTTGCCATCGAAACCCGGTCTCGGCGATCTCCCGGAGAGCTGCGCGGCGCTGATTCTGGGGTATTTGGACCCGCCGGAGATTTGCAAATTGGCGAATCTGAATCGGGCGTTTCGTGGCGCTTCTTGGGCTGATTTCATTTGGGAATCGAAAATGCCGTCGAATTATCAAACTATTGTGGGTAAAGTGTTCGGCGATGGATTGGAAAATCTGGGTAAGAGAGATGTTTTTACGAGGCTTTGTCAGCCTAATTCTTTCGATGATGGTACCAAGACAGTTTGGCTGGATAAAAGTACAGGATCGGTTTGCCTGTCAATTTCGTCGAAGGGATTAGCAATTACCGGCATTGATGATCGAAGATATTGGAATCATATTCCAACTGAAGAATCTAGATTTTGCTCAGTTGCATATCTTCAGCAAATATGGTGGTTTGAAGTTGATGGGGAGGTTGAGTTCCCGTTTCCTGTCGGGACATACAGCCTATTCTTCAGGTTGCAGCTGGGACGGTCTTCACAGAAGAGGTTTGGTCGCCGAGTTTGCAACATGGAGCATGTCCACGGTTGGGACATAAAACCTGTGAGGTTCCAGCTTTGGACTTCAGAAGGTCACTATGCCTCGTCTCAATGCTTTTTAACCGAACCTGGAAAATGGAATTACTACCATGTTGGGGACTTTGTTGTGGAGAAGCCGAATGCATCAACGAAGATTAAACTCTGTATGACCCAGATTGATTGCACCCACACCAAAGGCGGTCTCTGTTTAGACTCTGTACTGATATACCCTAGCGAATTTAAAGAAAGGCTAAAGCAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

419

Amino Acids

47.5

Weight (kDa)

8.69

Isoelectric Point (pI)

55.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 249 - 408 1.8e-36 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12710 AT1G12710 AT1G63090
fragaria_vesca FvH4_5g01540
malus_domestica MD04G1067200.v1.1 MD06G1073200.v1.1
prunus_persica Prupe.5G091600_v2.0.a1
pyrus_communis pycom04g06030
rosa_chinensis RchiOBHm_Chr7g0199151
rosa_laevigata RLG00000003810
rosa_multiflora Rmu_sc0006266.1_g000060
rosa_roxburghii Rroxscaffold_3G00257010
rosa_rugosa Rorug07G0053000
rosa_samantha Rh7BG180200 Rh7CG185800 Rh7DG179600
rosa_wichuraiana Rw7G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 707
AccB1I GGYRCC 2 cut(s) 392, 707
AccII CGCG 2 cut(s) 244, 488
AciI CCGC 5 cut(s) 169, 244, 488, 515, 1191
AclWI GGATC 3 cut(s) 34, 47, 748
AcsI RAATTY 1 cut(s) 1229
AcuI CTGAAG 4 cut(s) 839, 843, 913, 1023
AfaI GTAC 7 cut(s) 5, 9, 16, 241, 709, 736, 1212
AfiI CCNNNNNNNGG 3 cut(s) 464, 1003, 1108
AgsI TTSAA 2 cut(s) 349, 875
AjnI CCWGG 1 cut(s) 1081
AloI GAACNNNNNNTCC 2 cut(s) 878, 910
AluBI AGCT 6 cut(s) 82, 231, 352, 483, 940, 1030
AluI AGCT 6 cut(s) 82, 231, 352, 483, 940, 1030
Alw26I GTCTC 5 cut(s) 362, 415, 467, 1065, 1199
AlwI GGATC 3 cut(s) 34, 47, 748
Ama87I CYCGRG 1 cut(s) 195
AoxI GGCC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 483, 937
ApoI RAATTY 1 cut(s) 1229
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
Asp718I GGTACC 1 cut(s) 707
AspLEI GCGC 3 cut(s) 488, 493, 563
AspS9I GGNCC 2 cut(s) 246, 511
AsuC2I CCSGG 2 cut(s) 459, 476
AsuHPI GGTGA 1 cut(s) 260
AvaI CYCGRG 1 cut(s) 195
AvaII GGWCC 1 cut(s) 511
BamHI GGATCC 1 cut(s) 39
BanI GGYRCC 2 cut(s) 392, 707
BbsI GAAGAC 1 cut(s) 942
BbvI GCAGC 2 cut(s) 470, 949
BccI CCATC 4 cut(s) 457, 632, 698, 875
BceAI ACGGC 1 cut(s) 583
BciT130I CCWGG 1 cut(s) 1083
BcnI CCSGG 2 cut(s) 459, 476
BcoDI GTCTC 5 cut(s) 362, 415, 467, 1065, 1199
BfaI CTAG 3 cut(s) 363, 831, 1224
BfoI RGCGCY 2 cut(s) 494, 564
BisI GCNGC 4 cut(s) 245, 484, 489, 938
BlsI GCNGC 4 cut(s) 246, 485, 490, 939
Bme1390I CCNGG 3 cut(s) 459, 476, 1083
Bme18I GGWCC 1 cut(s) 511
BmeT110I CYCGRG 1 cut(s) 195
BmgT120I GGNCC 2 cut(s) 246, 511
BmiI GGNNCC 6 cut(s) 41, 248, 394, 513, 709, 1025
BmrFI CCNGG 3 cut(s) 459, 476, 1083
BmrI ACTGGG 1 cut(s) 422
BmsI GCATC 2 cut(s) 115, 1145
BmuI ACTGGG 1 cut(s) 422
BpiI GAAGAC 1 cut(s) 942
BpuMI CCSGG 2 cut(s) 459, 476
BsaAI YACGTR 1 cut(s) 7
BsaI GGTCTC 2 cut(s) 467, 1199
BsaJI CCNNGG 1 cut(s) 997
BsaXI ACNNNNNCTCC 2 cut(s) 878, 908
Bsc4I CCNNNNNNNGG 3 cut(s) 464, 1003, 1108
Bse118I RCCGGY 1 cut(s) 782
Bse1I ACTGG 3 cut(s) 72, 396, 428
BseBI CCWGG 1 cut(s) 1083
BseDI CCNNGG 1 cut(s) 997
BseLI CCNNNNNNNGG 3 cut(s) 464, 1003, 1108
BseMII CTCAG 1 cut(s) 855
BseNI ACTGG 3 cut(s) 72, 396, 428
BseRI GAGGAG 3 cut(s) 132, 327, 394
BseXI GCAGC 2 cut(s) 470, 949
BseYI CCCAGC 1 cut(s) 940
Bsh1236I CGCG 2 cut(s) 244, 488
BshFI GGCC 1 cut(s) 247
BshNI GGYRCC 2 cut(s) 392, 707
BsiHKCI CYCGRG 1 cut(s) 195
BsiSI CCGG 4 cut(s) 459, 476, 518, 783
BslFI GGGAC 4 cut(s) 925, 957, 1019, 1124
BslI CCNNNNNNNGG 3 cut(s) 464, 1003, 1108
BsmAI GTCTC 5 cut(s) 362, 415, 467, 1065, 1199
BsmBI CGTCTC 1 cut(s) 1065
BsmFI GGGAC 4 cut(s) 925, 957, 1019, 1124
BsmI GAATGC 1 cut(s) 1138
BsnI GGCC 1 cut(s) 247
Bso31I GGTCTC 2 cut(s) 467, 1199
BsoBI CYCGRG 1 cut(s) 195
Bsp143I GATC 4 cut(s) 39, 469, 740, 793
BspACI CCGC 5 cut(s) 169, 244, 488, 515, 1191
BspANI GGCC 1 cut(s) 247
BspCNI CTCAG 1 cut(s) 854
BspFNI CGCG 2 cut(s) 244, 488
BspLI GGNNCC 6 cut(s) 41, 248, 394, 513, 709, 1025
BspPI GGATC 3 cut(s) 34, 47, 748
BspT107I GGYRCC 2 cut(s) 392, 707
BspTNI GGTCTC 2 cut(s) 467, 1199
BsrFI RCCGGY 1 cut(s) 782
BsrI ACTGG 3 cut(s) 72, 396, 428
BssAI RCCGGY 1 cut(s) 782
BssECI CCNNGG 1 cut(s) 997
BssMI GATC 4 cut(s) 39, 469, 740, 793
Bst2UI CCWGG 1 cut(s) 1083
Bst4CI ACNGT 5 cut(s) 50, 57, 718, 948, 1001
Bst6I CTCTTC 1 cut(s) 953
BstBAI YACGTR 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 83, 841
BstDSI CCRYGG 1 cut(s) 997
BstFNI CGCG 2 cut(s) 244, 488
BstH2I RGCGCY 2 cut(s) 494, 564
BstHHI GCGC 3 cut(s) 488, 493, 563
BstKTI GATC 4 cut(s) 42, 472, 743, 796
BstMAI GTCTC 5 cut(s) 362, 415, 467, 1065, 1199
BstMBI GATC 4 cut(s) 39, 469, 740, 793
BstMWI GCNNNNNNNGC 3 cut(s) 569, 685, 978
BstNI CCWGG 1 cut(s) 1083
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 3 cut(s) 457, 474, 1081
BstSNI TACGTA 1 cut(s) 7
BstUI CGCG 2 cut(s) 244, 488
BstV1I GCAGC 2 cut(s) 470, 949
BstV2I GAAGAC 1 cut(s) 942
BstX2I RGATCY 1 cut(s) 39
BstYI RGATCY 1 cut(s) 39
BsuRI GGCC 1 cut(s) 247
BtgI CCRYGG 1 cut(s) 997
BtgZI GCGATG 1 cut(s) 651
CfoI GCGC 3 cut(s) 488, 493, 563
Cfr10I RCCGGY 1 cut(s) 782
Cfr13I GGNCC 2 cut(s) 246, 511
Csp6I GTAC 7 cut(s) 4, 8, 15, 240, 708, 735, 1211
CspCI CAANNNNNGTGG 2 cut(s) 848, 883
CviAII CATG 4 cut(s) 306, 985, 992, 1103
CviQI GTAC 7 cut(s) 4, 8, 15, 240, 708, 735, 1211
DdeI CTNAG 2 cut(s) 83, 841
DpnI GATC 4 cut(s) 41, 471, 742, 795
DpnII GATC 4 cut(s) 39, 469, 740, 793
DraI TTTAAA 1 cut(s) 1234
Eam1104I CTCTTC 1 cut(s) 953
EarI CTCTTC 1 cut(s) 953
Eco105I TACGTA 1 cut(s) 7
Eco31I GGTCTC 2 cut(s) 467, 1199
Eco47I GGWCC 1 cut(s) 511
Eco57I CTGAAG 4 cut(s) 839, 843, 913, 1023
Eco88I CYCGRG 1 cut(s) 195
EcoRII CCWGG 1 cut(s) 1081
EcoT22I ATGCAT 1 cut(s) 1138
Esp3I CGTCTC 1 cut(s) 1065
FaeI CATG 4 cut(s) 309, 988, 995, 1106
FaqI GGGAC 4 cut(s) 925, 957, 1019, 1124
FatI CATG 4 cut(s) 305, 984, 991, 1102
FauI CCCGC 1 cut(s) 522
Fnu4HI GCNGC 4 cut(s) 245, 484, 489, 938
Fsp4HI GCNGC 4 cut(s) 245, 484, 489, 938
FspBI CTAG 3 cut(s) 363, 831, 1224
GlaI GCGC 3 cut(s) 487, 492, 562
GluI GCNGC 4 cut(s) 245, 484, 489, 938
GsaI CCCAGC 1 cut(s) 944
HaeII RGCGCY 2 cut(s) 494, 564
HaeIII GGCC 1 cut(s) 247
HapII CCGG 4 cut(s) 459, 476, 518, 783
HhaI GCGC 3 cut(s) 488, 493, 563
Hin1II CATG 4 cut(s) 309, 988, 995, 1106
Hin6I GCGC 3 cut(s) 486, 491, 561
HinP1I GCGC 3 cut(s) 486, 491, 561
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 8 cut(s) 32, 496, 538, 544, 587, 808, 827, 1205
HpaII CCGG 4 cut(s) 459, 476, 518, 783
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 4 cut(s) 53, 77, 311, 997
Hpy188I TCNGA 4 cut(s) 164, 421, 543, 1042
Hpy188III TCNNGA 4 cut(s) 197, 363, 831, 910
Hpy8I GTNNAC 4 cut(s) 53, 77, 311, 997
Hpy99I CGWCG 2 cut(s) 604, 766
HpyAV CCTTC 2 cut(s) 760, 1037
HpyCH4III ACNGT 5 cut(s) 50, 57, 718, 948, 1001
HpyCH4IV ACGT 2 cut(s) 6, 24
HpyCH4V TGCA 7 cut(s) 434, 528, 848, 937, 981, 1136, 1176
HpyF10VI GCNNNNNNNGC 3 cut(s) 569, 685, 978
HpyF3I CTNAG 2 cut(s) 83, 841
HpySE526I ACGT 2 cut(s) 6, 24
Hsp92II CATG 4 cut(s) 309, 988, 995, 1106
HspAI GCGC 3 cut(s) 486, 491, 561
KpnI GGTACC 1 cut(s) 711
Kzo9I GATC 4 cut(s) 39, 469, 740, 793
LmnI GCTCC 1 cut(s) 988
Lsp1109I GCAGC 2 cut(s) 470, 949
LweI GCATC 2 cut(s) 115, 1145
MaeI CTAG 3 cut(s) 363, 831, 1224
MaeII ACGT 2 cut(s) 6, 24
MaeIII GTNAC 1 cut(s) 1046
MalI GATC 4 cut(s) 41, 471, 742, 795
MboI GATC 4 cut(s) 39, 469, 740, 793
MboII GAAGA 9 cut(s) 31, 337, 810, 836, 845, 919, 942, 970, 1156
MflI RGATCY 1 cut(s) 39
MlyI GAGTC 1 cut(s) 1199
MmeI TCCRAC 1 cut(s) 842
Mph1103I ATGCAT 1 cut(s) 1138
MseI TTAA 3 cut(s) 1073, 1149, 1233
MspA1I CMGCKG 1 cut(s) 940
MspI CCGG 4 cut(s) 459, 476, 518, 783
MspR9I CCNGG 3 cut(s) 459, 476, 1083
MteI GCGCNGCGC 1 cut(s) 489
Mva1269I GAATGC 1 cut(s) 1138
MvaI CCWGG 1 cut(s) 1083
MvnI CGCG 2 cut(s) 244, 488
MwoI GCNNNNNNNGC 3 cut(s) 569, 685, 978
NciI CCSGG 2 cut(s) 459, 476
NdeII GATC 4 cut(s) 39, 469, 740, 793
NlaIII CATG 4 cut(s) 309, 988, 995, 1106
NlaIV GGNNCC 6 cut(s) 41, 248, 394, 513, 709, 1025
NmeAIII GCCGAG 2 cut(s) 444, 998
NmuCI GTSAC 1 cut(s) 1046
NsiI ATGCAT 1 cut(s) 1138
NspI RCATGY 1 cut(s) 995
PcsI WCGNNNNNNNCGW 2 cut(s) 553, 596
PctI GAATGC 1 cut(s) 1138
PfeI GAWTC 7 cut(s) 32, 496, 538, 544, 587, 808, 827
PfoI TCCNGGA 1 cut(s) 474
PkrI GCNGC 4 cut(s) 246, 485, 490, 939
PleI GAGTC 1 cut(s) 1199
PpsI GAGTC 1 cut(s) 1199
Ppu21I YACGTR 1 cut(s) 7
Psp6I CCWGG 1 cut(s) 1081
PspFI CCCAGC 1 cut(s) 940
PspGI CCWGG 1 cut(s) 1081
PspN4I GGNNCC 6 cut(s) 41, 248, 394, 513, 709, 1025
PspPI GGNCC 2 cut(s) 246, 511
PsuI RGATCY 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 940
RsaI GTAC 7 cut(s) 5, 9, 16, 241, 709, 736, 1212
RsaNI GTAC 7 cut(s) 4, 8, 15, 240, 708, 735, 1211
SaqAI TTAA 3 cut(s) 1073, 1149, 1233
SatI GCNGC 4 cut(s) 245, 484, 489, 938
Sau3AI GATC 4 cut(s) 39, 469, 740, 793
Sau96I GGNCC 2 cut(s) 246, 511
SchI GAGTC 1 cut(s) 1199
ScrFI CCNGG 3 cut(s) 459, 476, 1083
SfaNI GCATC 2 cut(s) 115, 1145
SinI GGWCC 1 cut(s) 511
SnaBI TACGTA 1 cut(s) 7
SsiI CCGC 5 cut(s) 169, 244, 488, 515, 1191
SspMI CTAG 3 cut(s) 363, 831, 1224
StyD4I CCNGG 3 cut(s) 457, 474, 1081
TaaI ACNGT 5 cut(s) 50, 57, 718, 948, 1001
TaiI ACGT 2 cut(s) 9, 27
TaqI TCGA 6 cut(s) 452, 590, 602, 699, 764, 796
TatI WGTACW 2 cut(s) 734, 1210
TauI GCSGC 2 cut(s) 247, 491
TfiI GAWTC 7 cut(s) 32, 496, 538, 544, 587, 808, 827
Tru1I TTAA 3 cut(s) 1073, 1149, 1233
Tru9I TTAA 3 cut(s) 1073, 1149, 1233
TseFI GTSAC 1 cut(s) 1046
TseI GCWGC 2 cut(s) 483, 937
Tsp45I GTSAC 1 cut(s) 1046
TspDTI ATGAA 2 cut(s) 267, 568
TspGWI ACGGA 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 511
XapI RAATTY 1 cut(s) 1229
XbaI TCTAGA 2 cut(s) 362, 830
XceI RCATGY 1 cut(s) 995
XspI CTAG 3 cut(s) 363, 831, 1224
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.