Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0010128

Physical Location & Seq

Genomic Coordinates Forward (+)

7842231 .. 7843939

Gene Structure 1709 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1233 bp

ATGAAGGGGACTGATGCAGACAACCCTGAAATCACGATTGAAAGGGCTGCAAGTGATGAGGAACCATTTGCTGGGCTAGCATTCAAGATCATGAGTGATCCATTTGTGGGATCCCTTACATTTGTGAGAATATATGCAGGAAAGCTAACTGCTGGATCCTATGTGTTAAATGCAAACAAGGGAAAGAAGGAGAGGATTGGTAGACTTCTAGAAATGCATGCAAACAGTAGGGAGGATGTAAAGGTAGCTTTAGCTGGTGATATCGTTGCACTTGCAGGCCTGAAGGATACCATTACCGGTGAAACATTGAGTGACCCCGATCATCCTATTGTGCTCGAACGAATGGACTTCCCTGATCCTGTAATTAAGGTTGCCATTGAACCCAAAACTAAAGCTGATGTTGATAAGATGGGAGCTGGTTTGGTTAAGCTTGCTCAAGAAGACCCTTCCTTCCACTTCTCTCGGGATGAAGAGATCAACCAGACAGTGATTGAAGGAATGGGAGAATTGCATCTTGAGATTATTGTTGATCGGCTCAAGAGAGAATTCAAGGTTGAAGCAAATGTTGGTGCACCCCAGGTAAACTACCGGGAAAGCATTTCGAGAACCTCTGAAGTGAGGTATGTGCACAAGAAACAATCAGGTGGACAAGGTCAATTTGCAGATGTCACCGTACGGTTTGAACCAATGGAGGCTGGCGGAGGATATGAGTTTAAGAGTGAAATCAAGGGAGGTGCAGTGCCAAGAGAATACATTCCTGGGGTGATGAAAGGATTAGAGGAGTGTATGAGCAACGGTGTGCTTGCAGGCTTTCCTGTTGTTGACGTACGCGCTGTACTAGTTGATGGTTCTTACCATGATGTTGACTCAAGTGTGTTAGCATTTCAATTGGCAGCAAGAGGAGCTTTCCGGGAGGGGATAAAGAAAGCTGCCCCGAAAATGCTTGAACCAATAATGAAAGTTGAAGTTGTTACACCAGAAGAACATCTCGGAGATGTGATTGGTGATCTTAACTCGAGGAGAGGACAGATCAACAGCTTCAATGACAAACCTGGTGGTCTCAAGGTGGTGGATTCACTGGTTCCTCTTGCGGAGATGTTTCAGTACGTGAGTACGCTGAGGGGCATGACGAAAGGTCGTGCATCGTATACAATGCAGTTAGCTAAGTTTGACGTTGTTCCTCAGCACATTCAGAACCAGCTTGCTGCCAAGGAGGAAGAAGTTGCTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006996 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009791 GO:0009845 GO:0009941 GO:0009987 GO:0016043 GO:0017076 GO:0030554 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048856 GO:0071840 GO:0090351 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

411

Amino Acids

44.76

Weight (kDa)

5.33

Isoelectric Point (pI)

34.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-G_D2	PF22042	22 - 104	7.1e-17	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	37 - 103	6.4e-17	Elongation factor Tu domain 2
EFG_III	PF14492	117 - 190	4.9e-32	Elongation Factor G, domain III
RF3_C	PF16658	127 - 205	9.1e-07	Class II release factor RF3, C-terminal domain
EFG_IV	PF03764	192 - 310	5.2e-46	Elongation factor G, domain IV
EFG_C	PF00679	314 - 400	1.9e-26	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62750
fragaria_vesca	FvH4_5g00390 FvH4_5g00390 FvH4_5g00390 FvH4_5g00390
malus_domestica	MD04G1081800.v1.1 MD06G1065300.v1.1
prunus_persica	Prupe.5G076600_v2.0.a1 Prupe.5G076600_v2.0.a1
pyrus_communis	pycom04g07370 pycom06g05630
rosa_chinensis	RchiOBHm_Chr7g0202121
rosa_laevigata	RLG00000003624
rosa_multiflora	Rmu_ssc0000228.1_g000002
rosa_roxburghii	Rroxscaffold_3G00254840
rosa_rugosa	Rorug07G0068100
rosa_samantha	Rh7AG195600 Rh7BG196900 Rh7CG206700 Rh7DG202200
rosa_wichuraiana	Rw7G017130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	71
AccI	GTMKAC	2 cut(s)	202, 1148
AccII	CGCG	1 cut(s)	831
AciI	CCGC	2 cut(s)	699, 1091
AclWI	GGATC	6 cut(s)	92, 105, 118, 150, 163, 350
AcsI	RAATTY	1 cut(s)	545
AcuI	CTGAAG	2 cut(s)	302, 633
AfaI	GTAC	5 cut(s)	675, 828, 837, 1106, 1114
AfiI	CCNNNNNNNGG	2 cut(s)	71, 107
AgeI	ACCGGT	1 cut(s)	296
AhdI	GACNNNNNGTC	1 cut(s)	1134
AhlI	ACTAGT	1 cut(s)	838
AjnI	CCWGG	3 cut(s)	576, 757, 1051
AjuI	GAANNNNNNNTTGG	2 cut(s)	549, 581
Alw21I	GWGCWC	3 cut(s)	336, 574, 630
Alw26I	GTCTC	1 cut(s)	1064
Alw44I	GTGCAC	2 cut(s)	570, 626
AlwI	GGATC	6 cut(s)	92, 105, 118, 150, 163, 350
Ama87I	CYCGRG	2 cut(s)	462, 1015
AoxI	GGCC	1 cut(s)	277
ApaLI	GTGCAC	2 cut(s)	570, 626
ApeKI	GCWGC	5 cut(s)	47, 893, 929, 1205, 1226
ApoI	RAATTY	1 cut(s)	545
AsiGI	ACCGGT	1 cut(s)	296
Asp700I	GAANNNNTTC	1 cut(s)	753
AspLEI	GCGC	1 cut(s)	833
AsuC2I	CCSGG	2 cut(s)	590, 911
AsuHPI	GGTGA	5 cut(s)	269, 311, 661, 775, 1016
AsuNHI	GCTAGC	1 cut(s)	76
AvaI	CYCGRG	2 cut(s)	462, 1015
BaeGI	GKGCMC	2 cut(s)	574, 630
BamHI	GGATCC	2 cut(s)	110, 155
BbsI	GAAGAC	1 cut(s)	447
Bbv12I	GWGCWC	3 cut(s)	336, 574, 630
BbvCI	CCTCAGC	2 cut(s)	1118, 1182
BbvI	GCAGC	5 cut(s)	34, 905, 916, 1192, 1213
BccI	CCATC	2 cut(s)	403, 839
BciT130I	CCWGG	3 cut(s)	578, 759, 1053
BciVI	GTATCC	1 cut(s)	280
BcnI	CCSGG	2 cut(s)	590, 911
BcoDI	GTCTC	1 cut(s)	1064
BcuI	ACTAGT	1 cut(s)	838
BfaI	CTAG	3 cut(s)	77, 209, 839
BfuI	GTATCC	1 cut(s)	280
BisI	GCNGC	5 cut(s)	48, 894, 930, 1206, 1227
BlsI	GCNGC	5 cut(s)	49, 895, 931, 1207, 1228
Bme1390I	CCNGG	5 cut(s)	578, 590, 759, 911, 1053
BmeRI	GACNNNNNGTC	1 cut(s)	1134
BmeT110I	CYCGRG	2 cut(s)	462, 1015
BmiI	GGNNCC	4 cut(s)	63, 112, 157, 1083
BmrFI	CCNGG	5 cut(s)	578, 590, 759, 911, 1053
BmsI	GCATC	3 cut(s)	4, 520, 1151
BmtI	GCTAGC	1 cut(s)	80
BpiI	GAAGAC	1 cut(s)	447
Bpu10I	CCTNAGC	2 cut(s)	1118, 1182
BpuEI	CTTGAG	5 cut(s)	420, 521, 536, 853, 1046
BpuMI	CCSGG	2 cut(s)	590, 911
BsaAI	YACGTR	1 cut(s)	1108
BsaI	GGTCTC	1 cut(s)	1064
BsaJI	CCNNGG	3 cut(s)	576, 758, 1209
BsaWI	WCCGGW	1 cut(s)	296
BsaXI	ACNNNNNCTCC	2 cut(s)	723, 753
Bsc4I	CCNNNNNNNGG	2 cut(s)	71, 107
Bse118I	RCCGGY	1 cut(s)	296
Bse1I	ACTGG	1 cut(s)	1083
BseBI	CCWGG	3 cut(s)	578, 759, 1053
BseDI	CCNNGG	3 cut(s)	576, 758, 1209
BseGI	GGATG	3 cut(s)	241, 322, 472
BseLI	CCNNNNNNNGG	2 cut(s)	71, 107
BseMII	CTCAG	2 cut(s)	1109, 1196
BseNI	ACTGG	1 cut(s)	1083
BseRI	GAGGAG	3 cut(s)	794, 915, 1033
BseSI	GKGCMC	2 cut(s)	574, 630
BseXI	GCAGC	5 cut(s)	34, 905, 916, 1192, 1213
BseYI	CCCAGC	1 cut(s)	71
BsgI	GTGCAG	1 cut(s)	756
Bsh1236I	CGCG	1 cut(s)	831
BshFI	GGCC	1 cut(s)	279
BshTI	ACCGGT	1 cut(s)	296
BsiHKAI	GWGCWC	3 cut(s)	336, 574, 630
BsiHKCI	CYCGRG	2 cut(s)	462, 1015
BsiSI	CCGG	3 cut(s)	297, 589, 910
BsiWI	CGTACG	2 cut(s)	673, 826
BslFI	GGGAC	1 cut(s)	22
BslI	CCNNNNNNNGG	2 cut(s)	71, 107
BsmAI	GTCTC	1 cut(s)	1064
BsmFI	GGGAC	1 cut(s)	22
BsmI	GAATGC	1 cut(s)	80
BsnI	GGCC	1 cut(s)	279
Bso31I	GGTCTC	1 cut(s)	1064
BsoBI	CYCGRG	2 cut(s)	462, 1015
Bsp1286I	GDGCHC	3 cut(s)	336, 574, 630
BspACI	CCGC	2 cut(s)	699, 1091
BspANI	GGCC	1 cut(s)	279
BspCNI	CTCAG	2 cut(s)	1110, 1195
BspFNI	CGCG	1 cut(s)	831
BspHI	TCATGA	1 cut(s)	90
BspLI	GGNNCC	4 cut(s)	63, 112, 157, 1083
BspOI	GCTAGC	1 cut(s)	80
BspPI	GGATC	6 cut(s)	92, 105, 118, 150, 163, 350
BspTNI	GGTCTC	1 cut(s)	1064
BsrFI	RCCGGY	1 cut(s)	296
BsrI	ACTGG	1 cut(s)	1083
BssAI	RCCGGY	1 cut(s)	296
BssECI	CCNNGG	3 cut(s)	576, 758, 1209
BssNAI	GTATAC	1 cut(s)	1149
BssT1I	CCWWGG	1 cut(s)	1209
Bst1107I	GTATAC	1 cut(s)	1149
Bst2UI	CCWGG	3 cut(s)	578, 759, 1053
Bst4CI	ACNGT	5 cut(s)	227, 487, 673, 678, 797
Bst6I	CTCTTC	1 cut(s)	465
BstBAI	YACGTR	1 cut(s)	1108
BstC8I	GCNNGC	8 cut(s)	78, 219, 277, 432, 697, 804, 808, 1203
BstDEI	CTNAG	3 cut(s)	1118, 1164, 1182
BstF5I	GGATG	3 cut(s)	241, 322, 472
BstFNI	CGCG	1 cut(s)	831
BstHHI	GCGC	1 cut(s)	833
BstMAI	GTCTC	1 cut(s)	1064
BstMWI	GCNNNNNNNGC	2 cut(s)	77, 902
BstNI	CCWGG	3 cut(s)	578, 759, 1053
BstNSI	RCATGY	1 cut(s)	221
BstSCI	CCNGG	5 cut(s)	576, 588, 757, 909, 1051
BstSLI	GKGCMC	2 cut(s)	574, 630
BstUI	CGCG	1 cut(s)	831
BstV1I	GCAGC	5 cut(s)	34, 905, 916, 1192, 1213
BstV2I	GAAGAC	1 cut(s)	447
BstX2I	RGATCY	2 cut(s)	110, 155
BstYI	RGATCY	2 cut(s)	110, 155
BstZ17I	GTATAC	1 cut(s)	1149
BsuI	GTATCC	1 cut(s)	280
BsuRI	GGCC	1 cut(s)	279
BtsCI	GGATG	3 cut(s)	241, 322, 472
BtsI	GCAGTG	1 cut(s)	744
BtsIMutI	CAGTG	3 cut(s)	492, 744, 1076
Cac8I	GCNNGC	8 cut(s)	78, 219, 277, 432, 697, 804, 808, 1203
CciI	TCATGA	1 cut(s)	90
CfoI	GCGC	1 cut(s)	833
Cfr10I	RCCGGY	1 cut(s)	296
CsiI	ACCWGGT	1 cut(s)	1051
Csp6I	GTAC	5 cut(s)	674, 827, 836, 1105, 1113
CspAI	ACCGGT	1 cut(s)	296
CspCI	CAANNNNNGTGG	2 cut(s)	1036, 1071
CviAII	CATG	4 cut(s)	91, 218, 857, 1126
CviQI	GTAC	5 cut(s)	674, 827, 836, 1105, 1113
DdeI	CTNAG	3 cut(s)	1118, 1164, 1182
DriI	GACNNNNNGTC	1 cut(s)	1134
Eam1104I	CTCTTC	1 cut(s)	465
Eam1105I	GACNNNNNGTC	1 cut(s)	1134
EarI	CTCTTC	1 cut(s)	465
EciI	GGCGGA	1 cut(s)	714
Eco130I	CCWWGG	1 cut(s)	1209
Eco147I	AGGCCT	1 cut(s)	279
Eco31I	GGTCTC	1 cut(s)	1064
Eco32I	GATATC	1 cut(s)	262
Eco57I	CTGAAG	2 cut(s)	302, 633
Eco88I	CYCGRG	2 cut(s)	462, 1015
EcoRI	GAATTC	1 cut(s)	545
EcoRII	CCWGG	3 cut(s)	576, 757, 1051
EcoRV	GATATC	1 cut(s)	262
EcoT14I	CCWWGG	1 cut(s)	1209
EcoT22I	ATGCAT	1 cut(s)	219
ErhI	CCWWGG	1 cut(s)	1209
FaeI	CATG	4 cut(s)	94, 221, 860, 1129
FalI	AAGNNNNNCTT	2 cut(s)	889, 921
FaqI	GGGAC	1 cut(s)	22
FatI	CATG	4 cut(s)	90, 217, 856, 1125
FblI	GTMKAC	2 cut(s)	202, 1148
Fnu4HI	GCNGC	5 cut(s)	48, 894, 930, 1206, 1227
FokI	GGATG	3 cut(s)	248, 309, 479
Fsp4HI	GCNGC	5 cut(s)	48, 894, 930, 1206, 1227
FspBI	CTAG	3 cut(s)	77, 209, 839
GlaI	GCGC	1 cut(s)	832
GluI	GCNGC	5 cut(s)	48, 894, 930, 1206, 1227
GsaI	CCCAGC	1 cut(s)	75
HaeIII	GGCC	1 cut(s)	279
HapII	CCGG	3 cut(s)	297, 589, 910
HhaI	GCGC	1 cut(s)	833
Hin1II	CATG	4 cut(s)	94, 221, 860, 1129
Hin6I	GCGC	1 cut(s)	831
HinP1I	GCGC	1 cut(s)	831
HincII	GTYRAC	2 cut(s)	823, 865
HindII	GTYRAC	2 cut(s)	823, 865
HindIII	AAGCTT	1 cut(s)	428
HinfI	GANTC	2 cut(s)	866, 1073
HpaII	CCGG	3 cut(s)	297, 589, 910
HphI	GGTGA	5 cut(s)	269, 311, 661, 775, 1016
Hpy166II	GTNNAC	8 cut(s)	203, 572, 583, 628, 647, 823, 865, 1149
Hpy188I	TCNGA	3 cut(s)	613, 992, 1194
Hpy188III	TCNNGA	9 cut(s)	34, 85, 91, 209, 437, 464, 515, 538, 603
Hpy8I	GTNNAC	8 cut(s)	203, 572, 583, 628, 647, 823, 865, 1149
HpyAV	CCTTC	5 cut(s)	181, 277, 456, 460, 488
HpyCH4III	ACNGT	5 cut(s)	227, 487, 673, 678, 797
HpyCH4IV	ACGT	3 cut(s)	825, 1107, 1173
HpyF10VI	GCNNNNNNNGC	2 cut(s)	77, 902
HpyF3I	CTNAG	3 cut(s)	1118, 1164, 1182
HpySE526I	ACGT	3 cut(s)	825, 1107, 1173
Hsp92II	CATG	4 cut(s)	94, 221, 860, 1129
HspAI	GCGC	1 cut(s)	831
LmnI	GCTCC	2 cut(s)	413, 902
Lsp1109I	GCAGC	5 cut(s)	34, 905, 916, 1192, 1213
LweI	GCATC	3 cut(s)	4, 520, 1151
MabI	ACCWGGT	1 cut(s)	1051
MaeI	CTAG	3 cut(s)	77, 209, 839
MaeII	ACGT	3 cut(s)	825, 1107, 1173
MaeIII	GTNAC	3 cut(s)	311, 667, 970
MboII	GAAGA	4 cut(s)	452, 482, 992, 1229
MfeI	CAATTG	1 cut(s)	887
MflI	RGATCY	2 cut(s)	110, 155
MhlI	GDGCHC	3 cut(s)	336, 574, 630
MluCI	AATT	5 cut(s)	363, 506, 545, 656, 887
MlyI	GAGTC	1 cut(s)	860
Mph1103I	ATGCAT	1 cut(s)	219
MroXI	GAANNNNTTC	1 cut(s)	753
MseI	TTAA	5 cut(s)	167, 366, 426, 714, 1011
MspI	CCGG	3 cut(s)	297, 589, 910
MspR9I	CCNGG	5 cut(s)	578, 590, 759, 911, 1053
MunI	CAATTG	1 cut(s)	887
Mva1269I	GAATGC	1 cut(s)	80
MvaI	CCWGG	3 cut(s)	578, 759, 1053
MvnI	CGCG	1 cut(s)	831
MwoI	GCNNNNNNNGC	2 cut(s)	77, 902
NciI	CCSGG	2 cut(s)	590, 911
NheI	GCTAGC	1 cut(s)	76
NlaIII	CATG	4 cut(s)	94, 221, 860, 1129
NlaIV	GGNNCC	4 cut(s)	63, 112, 157, 1083
NmuCI	GTSAC	2 cut(s)	311, 667
NsiI	ATGCAT	1 cut(s)	219
NspI	RCATGY	1 cut(s)	221
PaeI	GCATGC	1 cut(s)	221
PaeR7I	CTCGAG	1 cut(s)	1015
PagI	TCATGA	1 cut(s)	90
PceI	AGGCCT	1 cut(s)	279
PctI	GAATGC	1 cut(s)	80
PdmI	GAANNNNTTC	1 cut(s)	753
PfeI	GAWTC	1 cut(s)	1073
Pfl23II	CGTACG	2 cut(s)	673, 826
PflFI	GACNNNGTC	1 cut(s)	651
PflMI	CCANNNNNTGG	1 cut(s)	71
PfoI	TCCNGGA	1 cut(s)	909
PinAI	ACCGGT	1 cut(s)	296
PkrI	GCNGC	5 cut(s)	49, 895, 931, 1207, 1228
PleI	GAGTC	1 cut(s)	860
PpsI	GAGTC	1 cut(s)	860
Ppu21I	YACGTR	1 cut(s)	1108
Psp6I	CCWGG	3 cut(s)	576, 757, 1051
PspFI	CCCAGC	1 cut(s)	71
PspGI	CCWGG	3 cut(s)	576, 757, 1051
PspLI	CGTACG	2 cut(s)	673, 826
PspN4I	GGNNCC	4 cut(s)	63, 112, 157, 1083
PspXI	VCTCGAGB	1 cut(s)	1015
PsuI	RGATCY	2 cut(s)	110, 155
PsyI	GACNNNGTC	1 cut(s)	651
RsaI	GTAC	5 cut(s)	675, 828, 837, 1106, 1114
RsaNI	GTAC	5 cut(s)	674, 827, 836, 1105, 1113
SaqAI	TTAA	5 cut(s)	167, 366, 426, 714, 1011
SatI	GCNGC	5 cut(s)	48, 894, 930, 1206, 1227
SchI	GAGTC	1 cut(s)	860
ScrFI	CCNGG	5 cut(s)	578, 590, 759, 911, 1053
SduI	GDGCHC	3 cut(s)	336, 574, 630
SexAI	ACCWGGT	1 cut(s)	1051
SfaNI	GCATC	3 cut(s)	4, 520, 1151
Sfr274I	CTCGAG	1 cut(s)	1015
SlaI	CTCGAG	1 cut(s)	1015
SmlI	CTYRAG	6 cut(s)	435, 515, 536, 868, 1015, 1061
SmoI	CTYRAG	6 cut(s)	435, 515, 536, 868, 1015, 1061
SpeI	ACTAGT	1 cut(s)	838
SphI	GCATGC	1 cut(s)	221
Sse9I	AATT	5 cut(s)	363, 506, 545, 656, 887
SseBI	AGGCCT	1 cut(s)	279
SsiI	CCGC	2 cut(s)	699, 1091
SspMI	CTAG	3 cut(s)	77, 209, 839
StuI	AGGCCT	1 cut(s)	279
StyD4I	CCNGG	5 cut(s)	576, 588, 757, 909, 1051
StyI	CCWWGG	1 cut(s)	1209
TaaI	ACNGT	5 cut(s)	227, 487, 673, 678, 797
TaiI	ACGT	3 cut(s)	828, 1110, 1176
TaqI	TCGA	3 cut(s)	336, 602, 1016
TasI	AATT	5 cut(s)	363, 506, 545, 656, 887
TatI	WGTACW	1 cut(s)	835
TfiI	GAWTC	1 cut(s)	1073
Tru1I	TTAA	5 cut(s)	167, 366, 426, 714, 1011
Tru9I	TTAA	5 cut(s)	167, 366, 426, 714, 1011
TscAI	CASTG	3 cut(s)	492, 744, 1083
TseFI	GTSAC	2 cut(s)	311, 667
TseI	GCWGC	5 cut(s)	47, 893, 929, 1205, 1226
Tsp45I	GTSAC	2 cut(s)	311, 667
TspDTI	ATGAA	4 cut(s)	17, 483, 782, 971
TspRI	CASTG	3 cut(s)	492, 744, 1083
Tth111I	GACNNNGTC	1 cut(s)	651
Van91I	CCANNNNNTGG	1 cut(s)	71
VneI	GTGCAC	2 cut(s)	570, 626
XapI	RAATTY	1 cut(s)	545
XbaI	TCTAGA	1 cut(s)	208
XceI	RCATGY	1 cut(s)	221
XhoI	CTCGAG	1 cut(s)	1015
XmiI	GTMKAC	2 cut(s)	202, 1148
XmnI	GAANNNNTTC	1 cut(s)	753
XspI	CTAG	3 cut(s)	77, 209, 839
Zsp2I	ATGCAT	1 cut(s)	219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.