pycom04g11830

Rhomboid-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011657
Physical Location & Seq
Reverse (-)
14777807 .. 14780954
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1176 bp
ATGGCGGGGGAGCCACCGTCGTCGGGGGCCCAAATGAAAGTGAACTCACGGCGGCACCACAACGTAGTCCACCCAGTAGACATCGAGACGCCGCCTGTAGCCTCCCCAGCTCCGGCCGCGTCTCCGCCCGTGTACAGAGAGATCAAGCATTTTAAGAAATGGGTTGTCTGGCTGATTCCTGTATTTATTGCTGCCAATGTTGTTATGTTCATCATCACCATGTACGTCAACAACTGTCCCAAGAACTCGGTCTCTTGCATTGCCACGTTCTTGGGGAGGTTCTCCTTCCAGCCTTTTAAGGAGAACCCGCTTCTAGGACCCTCGTCATCGACGTTGCAGAAGATGGGGGCTTTGGATGTAAAGAAGGTGGTTGACAGACACCAGGGGTGGCTTCTAATCACTTGCAATTGGTTACATGCGGGCGTCTTCCATTTATTGGCTAATATGCTGAGCCTTTTAGTCATTGGATATCGGCTAGAGCAAGAGTTCGGCTTCGTCCGAATTGGGTTGCTATATGTCATCTCTGGACTTGGTGGGAGTTTGTTGTCTTCGCTTTTCATCCAAACAAACATTTCAGTTGGTGCTTCTGGTGCACTTTTCGGGTTGCTGGGAGCCATGCTTTCTGAACTTATCACTAATTGGACATTATATGCTAGTAAGTTTGGAGCACTTTTCACCATCCTGATCATCATAGCAATCAATTTGGCAGTGGGAATACTCCCACACGTTGACAACTTTGCTCATATTGGAGGATTCCTCTCGGGATTTTTTCTTGGGTTTGTGTTTCTTATCCGCCCCCAGTTCGGATGGGTTAACCAAAGATATGCTGCTCCTGGTCCAGGATATGCCTCATCTCAAACAAAATCGAAGTTCAAGACCTATCAGTGCATTCTGTGGGTCCTCTCTGTGATAGTTTTAATTGTTGGGTTTACAGTTGGCCTGGTTATGCTTCTTCGGGGAGTTGATGCCAATAAACATTGTTCTTGGTGTCATTATTTATCTTGTGTCCCTACTTCAAGATGGAGCTGCAACACGGAGCCTGCATACTGCACGTCAAACCAGATGGGTGATCAAATCTATTTAACATGCTCGAGCAATGGTAAGAACGGCACGTATGCGATTCCAAATGCAAGCAGTTCTCAGATCCAGGGGTTATGTTCTCAGCTTTGCAGTTGA

Protein Analysis

392

Amino Acids

42.73

Weight (kDa)

9.2

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 127 - 263 1.7e-40 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011657)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53780 AT3G53780 AT3G53780
fragaria_vesca FvH4_6g13560
malus_domestica MD04G1130000.v1.1 MD12G1143100.v1.1
prunus_persica Prupe.6G256700_v2.0.a1
pyrus_communis pycom04g11830 pycom12g13780
rosa_chinensis RchiOBHm_Chr3g0466421
rosa_laevigata RLG00000024575
rosa_multiflora Rmu_co8379997.1_g000002 Rmu_sc0003332.1_g000027 Rmu_sc0003480.1_g000001
rosa_roxburghii Rroxscaffold_6G00414480
rosa_samantha Rh3AG128300 Rh3BG149600 Rh3DG150300
rosa_wichuraiana Rw3G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 54
AccB7I CCANNNNNTGG 1 cut(s) 436
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 119
AciI CCGC 8 cut(s) 5, 52, 92, 117, 125, 308, 419, 793
AclWI GGATC 1 cut(s) 1138
AcoI YGGCCR 1 cut(s) 114
AcyI GRCGYC 2 cut(s) 89, 423
AfaI GTAC 2 cut(s) 134, 224
AfiI CCNNNNNNNGG 6 cut(s) 23, 112, 314, 436, 803, 839
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 2 cut(s) 874, 1017
AjiI CACGTC 1 cut(s) 1053
AjnI CCWGG 5 cut(s) 381, 832, 838, 939, 1146
AluBI AGCT 3 cut(s) 110, 1026, 1165
AluI AGCT 3 cut(s) 110, 1026, 1165
Alw21I GWGCWC 2 cut(s) 595, 670
Alw26I GTCTC 3 cut(s) 80, 126, 256
Alw44I GTGCAC 1 cut(s) 591
AlwI GGATC 1 cut(s) 1138
Ama87I CYCGRG 2 cut(s) 760, 1090
AoxI GGCC 3 cut(s) 27, 114, 937
ApaI GGGCCC 1 cut(s) 31
ApaLI GTGCAC 1 cut(s) 591
ApeKI GCWGC 3 cut(s) 191, 827, 1026
AspS9I GGNCC 5 cut(s) 27, 28, 317, 836, 898
AsuHPI GGTGA 3 cut(s) 208, 667, 1079
AvaI CYCGRG 2 cut(s) 760, 1090
AvaII GGWCC 3 cut(s) 317, 836, 898
BaeGI GKGCMC 2 cut(s) 31, 595
BanI GGYRCC 1 cut(s) 54
BanII GRGCYC 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 418, 540
Bbv12I GWGCWC 2 cut(s) 595, 670
BbvI GCAGC 3 cut(s) 178, 814, 1013
BccI CCATC 5 cut(s) 337, 686, 801, 1014, 1057
BceAI ACGGC 2 cut(s) 65, 1123
BciT130I CCWGG 5 cut(s) 383, 834, 840, 941, 1148
BclI TGATCA 2 cut(s) 684, 1069
BcoDI GTCTC 3 cut(s) 80, 126, 256
BfaI CTAG 3 cut(s) 314, 476, 654
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 6 cut(s) 53, 92, 117, 192, 828, 1027
BlpI GCTNAGC 1 cut(s) 449
BlsI GCNGC 6 cut(s) 54, 93, 118, 193, 829, 1028
Bme1390I CCNGG 5 cut(s) 383, 834, 840, 941, 1148
Bme18I GGWCC 3 cut(s) 317, 836, 898
BmeT110I CYCGRG 2 cut(s) 760, 1090
BmgBI CACGTC 1 cut(s) 1053
BmgT120I GGNCC 5 cut(s) 27, 28, 317, 836, 898
BmiI GGNNCC 8 cut(s) 12, 28, 29, 56, 319, 613, 899, 1038
BmrFI CCNGG 5 cut(s) 383, 834, 840, 941, 1148
BmrI ACTGGG 2 cut(s) 68, 793
BmsI GCATC 1 cut(s) 955
BmuI ACTGGG 2 cut(s) 68, 793
BoxI GACNNNNGTC 1 cut(s) 322
BpiI GAAGAC 2 cut(s) 418, 540
BplI GAGNNNNNCTC 2 cut(s) 741, 773
Bpu1102I GCTNAGC 1 cut(s) 449
BsaAI YACGTR 1 cut(s) 1113
BsaHI GRCGYC 2 cut(s) 89, 423
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 382, 1147
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 112, 314, 436, 803, 839
Bse1I ACTGG 2 cut(s) 74, 799
Bse3DI GCAATG 2 cut(s) 258, 1102
BseBI CCWGG 5 cut(s) 383, 834, 840, 941, 1148
BseDI CCNNGG 2 cut(s) 382, 1147
BseGI GGATG 4 cut(s) 361, 558, 678, 812
BseLI CCNNNNNNNGG 6 cut(s) 23, 112, 314, 436, 803, 839
BseMI GCAATG 2 cut(s) 258, 1102
BseMII CTCAG 3 cut(s) 440, 1154, 1175
BseNI ACTGG 2 cut(s) 74, 799
BseSI GKGCMC 2 cut(s) 31, 595
BseX3I CGGCCG 1 cut(s) 114
BseXI GCAGC 3 cut(s) 178, 814, 1013
BseYI CCCAGC 2 cut(s) 106, 607
BsgI GTGCAG 1 cut(s) 1033
Bsh1236I CGCG 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 3 cut(s) 29, 116, 939
BshNI GGYRCC 1 cut(s) 54
BsiEI CGRYCG 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 595, 670
BsiHKCI CYCGRG 2 cut(s) 760, 1090
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 2 cut(s) 222, 992
BslI CCNNNNNNNGG 6 cut(s) 23, 112, 314, 436, 803, 839
BsmAI GTCTC 3 cut(s) 80, 126, 256
BsmBI CGTCTC 2 cut(s) 80, 126
BsmFI GGGAC 2 cut(s) 222, 992
BsmI GAATGC 1 cut(s) 888
BsnI GGCC 3 cut(s) 29, 116, 939
Bso31I GGTCTC 1 cut(s) 256
BsoBI CYCGRG 2 cut(s) 760, 1090
Bsp120I GGGCCC 1 cut(s) 27
Bsp1286I GDGCHC 3 cut(s) 31, 595, 670
Bsp1407I TGTACA 1 cut(s) 132
Bsp143I GATC 4 cut(s) 141, 684, 1069, 1143
Bsp1720I GCTNAGC 1 cut(s) 449
BspACI CCGC 8 cut(s) 5, 52, 92, 117, 125, 308, 419, 793
BspANI GGCC 3 cut(s) 29, 116, 939
BspCNI CTCAG 3 cut(s) 441, 1153, 1174
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 8 cut(s) 12, 28, 29, 56, 319, 613, 899, 1038
BspPI GGATC 1 cut(s) 1138
BspT107I GGYRCC 1 cut(s) 54
BspTNI GGTCTC 1 cut(s) 256
BsrDI GCAATG 2 cut(s) 258, 1102
BsrGI TGTACA 1 cut(s) 132
BsrI ACTGG 2 cut(s) 74, 799
BssECI CCNNGG 2 cut(s) 382, 1147
BssMI GATC 4 cut(s) 141, 684, 1069, 1143
BssNI GRCGYC 2 cut(s) 89, 423
Bst2UI CCWGG 5 cut(s) 383, 834, 840, 941, 1148
Bst4CI ACNGT 3 cut(s) 18, 236, 934
BstACI GRCGYC 2 cut(s) 89, 423
BstAUI TGTACA 1 cut(s) 132
BstBAI YACGTR 1 cut(s) 1113
BstC8I GCNNGC 3 cut(s) 421, 1041, 1132
BstDEI CTNAG 3 cut(s) 449, 1140, 1161
BstENI CCTNNNNNAGG 1 cut(s) 837
BstF5I GGATG 4 cut(s) 361, 558, 678, 812
BstFNI CGCG 1 cut(s) 119
BstKTI GATC 4 cut(s) 144, 687, 1072, 1146
BstMAI GTCTC 3 cut(s) 80, 126, 256
BstMBI GATC 4 cut(s) 141, 684, 1069, 1143
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 3 cut(s) 107, 116, 590
BstNI CCWGG 5 cut(s) 383, 834, 840, 941, 1148
BstNSI RCATGY 2 cut(s) 419, 1089
BstPAI GACNNNNGTC 1 cut(s) 322
BstSCI CCNGG 5 cut(s) 381, 832, 838, 939, 1146
BstSFI CTRYAG 1 cut(s) 96
BstSLI GKGCMC 2 cut(s) 31, 595
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 3 cut(s) 178, 814, 1013
BstV2I GAAGAC 2 cut(s) 418, 540
BstX2I RGATCY 1 cut(s) 1143
BstXI CCANNNNNNTGG 1 cut(s) 271
BstYI RGATCY 1 cut(s) 1143
BstZI CGGCCG 1 cut(s) 114
BsuRI GGCC 3 cut(s) 29, 116, 939
BtrI CACGTC 1 cut(s) 1053
BtsCI GGATG 4 cut(s) 361, 558, 678, 812
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 2 cut(s) 714, 890
Cac8I GCNNGC 3 cut(s) 421, 1041, 1132
Cfr13I GGNCC 5 cut(s) 27, 28, 317, 836, 898
CseI GACGC 3 cut(s) 97, 108, 412
Csp6I GTAC 2 cut(s) 133, 223
CviAII CATG 4 cut(s) 220, 416, 616, 1086
CviQI GTAC 2 cut(s) 133, 223
DdeI CTNAG 3 cut(s) 449, 1140, 1161
DpnI GATC 4 cut(s) 143, 686, 1071, 1145
DpnII GATC 4 cut(s) 141, 684, 1069, 1143
EaeI YGGCCR 1 cut(s) 114
EagI CGGCCG 1 cut(s) 114
EciI GGCGGA 2 cut(s) 114, 782
EclXI CGGCCG 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 256
Eco32I GATATC 1 cut(s) 470
Eco47I GGWCC 3 cut(s) 317, 836, 898
Eco52I CGGCCG 1 cut(s) 114
Eco88I CYCGRG 2 cut(s) 760, 1090
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoO109I RGGNCCY 3 cut(s) 27, 317, 898
EcoRII CCWGG 5 cut(s) 381, 832, 838, 939, 1146
EcoRV GATATC 1 cut(s) 470
EcoT38I GRGCYC 1 cut(s) 31
Esp3I CGTCTC 2 cut(s) 80, 126
FaeI CATG 4 cut(s) 223, 419, 619, 1089
FaqI GGGAC 2 cut(s) 222, 992
FatI CATG 4 cut(s) 219, 415, 615, 1085
FauI CCCGC 2 cut(s) 315, 412
FbaI TGATCA 2 cut(s) 684, 1069
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 6 cut(s) 53, 92, 117, 192, 828, 1027
FokI GGATG 4 cut(s) 368, 545, 665, 819
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 6 cut(s) 53, 92, 117, 192, 828, 1027
FspBI CTAG 3 cut(s) 314, 476, 654
GluI GCNGC 6 cut(s) 53, 92, 117, 192, 828, 1027
GsaI CCCAGC 2 cut(s) 110, 611
HaeIII GGCC 3 cut(s) 29, 116, 939
HapII CCGG 1 cut(s) 113
HgaI GACGC 3 cut(s) 97, 108, 412
Hin1I GRCGYC 2 cut(s) 89, 423
Hin1II CATG 4 cut(s) 223, 419, 619, 1089
HincII GTYRAC 4 cut(s) 229, 373, 730, 814
HindII GTYRAC 4 cut(s) 229, 373, 730, 814
HinfI GANTC 3 cut(s) 175, 753, 1120
HpaI GTTAAC 1 cut(s) 814
HpaII CCGG 1 cut(s) 113
HphI GGTGA 3 cut(s) 208, 667, 1079
Hpy188I TCNGA 4 cut(s) 500, 625, 806, 1143
Hpy188III TCNNGA 6 cut(s) 85, 525, 682, 762, 874, 1017
Hpy99I CGWCG 3 cut(s) 22, 25, 334
HpyAV CCTTC 2 cut(s) 295, 358
HpyCH4III ACNGT 3 cut(s) 18, 236, 934
HpyCH4IV ACGT 7 cut(s) 63, 225, 266, 332, 726, 1052, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 116, 590
HpyF3I CTNAG 3 cut(s) 449, 1140, 1161
HpySE526I ACGT 7 cut(s) 63, 225, 266, 332, 726, 1052, 1112
Hsp92I GRCGYC 2 cut(s) 89, 423
Hsp92II CATG 4 cut(s) 223, 419, 619, 1089
Ksp22I TGATCA 2 cut(s) 684, 1069
KspAI GTTAAC 1 cut(s) 814
Kzo9I GATC 4 cut(s) 141, 684, 1069, 1143
LmnI GCTCC 7 cut(s) 10, 115, 611, 665, 835, 1023, 1036
Lsp1109I GCAGC 3 cut(s) 178, 814, 1013
LweI GCATC 1 cut(s) 955
MaeI CTAG 3 cut(s) 314, 476, 654
MaeII ACGT 7 cut(s) 63, 225, 266, 332, 726, 1052, 1112
MaeIII GTNAC 1 cut(s) 411
MalI GATC 4 cut(s) 143, 686, 1071, 1145
MboI GATC 4 cut(s) 141, 684, 1069, 1143
MboII GAAGA 4 cut(s) 352, 418, 540, 944
MfeI CAATTG 1 cut(s) 406
MflI RGATCY 1 cut(s) 1143
MhlI GDGCHC 3 cut(s) 31, 595, 670
MluCI AATT 5 cut(s) 406, 501, 637, 700, 918
MnlI CCTC 7 cut(s) 112, 270, 331, 743, 767, 859, 911
MseI TTAA 5 cut(s) 153, 297, 813, 917, 1082
MslI CAYNNNNRTG 1 cut(s) 218
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 5 cut(s) 383, 834, 840, 941, 1148
MunI CAATTG 1 cut(s) 406
Mva1269I GAATGC 1 cut(s) 888
MvaI CCWGG 5 cut(s) 383, 834, 840, 941, 1148
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 3 cut(s) 107, 116, 590
NdeII GATC 4 cut(s) 141, 684, 1069, 1143
NlaIII CATG 4 cut(s) 223, 419, 619, 1089
NlaIV GGNNCC 8 cut(s) 12, 28, 29, 56, 319, 613, 899, 1038
NspI RCATGY 2 cut(s) 419, 1089
PaeR7I CTCGAG 1 cut(s) 1090
PcsI WCGNNNNNNNCGW 1 cut(s) 329
PctI GAATGC 1 cut(s) 888
PfeI GAWTC 3 cut(s) 175, 753, 1120
PflMI CCANNNNNTGG 1 cut(s) 436
PfoI TCCNGGA 1 cut(s) 838
PkrI GCNGC 6 cut(s) 54, 93, 118, 193, 829, 1028
Ppu21I YACGTR 1 cut(s) 1113
PpuMI RGGWCCY 2 cut(s) 317, 898
PshAI GACNNNNGTC 1 cut(s) 322
Psp5II RGGWCCY 2 cut(s) 317, 898
Psp6I CCWGG 5 cut(s) 381, 832, 838, 939, 1146
PspFI CCCAGC 2 cut(s) 106, 607
PspGI CCWGG 5 cut(s) 381, 832, 838, 939, 1146
PspN4I GGNNCC 8 cut(s) 12, 28, 29, 56, 319, 613, 899, 1038
PspOMI GGGCCC 1 cut(s) 27
PspPI GGNCC 5 cut(s) 27, 28, 317, 836, 898
PspPPI RGGWCCY 2 cut(s) 317, 898
PspXI VCTCGAGB 1 cut(s) 1090
PsuI RGATCY 1 cut(s) 1143
RsaI GTAC 2 cut(s) 134, 224
RsaNI GTAC 2 cut(s) 133, 223
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 5 cut(s) 153, 297, 813, 917, 1082
SatI GCNGC 6 cut(s) 53, 92, 117, 192, 828, 1027
Sau3AI GATC 4 cut(s) 141, 684, 1069, 1143
Sau96I GGNCC 5 cut(s) 27, 28, 317, 836, 898
ScrFI CCNGG 5 cut(s) 383, 834, 840, 941, 1148
SduI GDGCHC 3 cut(s) 31, 595, 670
SfaNI GCATC 1 cut(s) 955
SfcI CTRYAG 1 cut(s) 96
Sfr274I CTCGAG 1 cut(s) 1090
SinI GGWCC 3 cut(s) 317, 836, 898
SlaI CTCGAG 1 cut(s) 1090
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 1090
SmoI CTYRAG 1 cut(s) 1090
Sse9I AATT 5 cut(s) 406, 501, 637, 700, 918
SsiI CCGC 8 cut(s) 5, 52, 92, 117, 125, 308, 419, 793
SspMI CTAG 3 cut(s) 314, 476, 654
StyD4I CCNGG 5 cut(s) 381, 832, 838, 939, 1146
TaaI ACNGT 3 cut(s) 18, 236, 934
TaiI ACGT 7 cut(s) 66, 228, 269, 335, 729, 1055, 1115
TaqI TCGA 4 cut(s) 84, 329, 866, 1091
TaqII GACCGA 1 cut(s) 238
TasI AATT 5 cut(s) 406, 501, 637, 700, 918
TatI WGTACW 1 cut(s) 132
TauI GCSGC 3 cut(s) 55, 94, 119
TfiI GAWTC 3 cut(s) 175, 753, 1120
Tru1I TTAA 5 cut(s) 153, 297, 813, 917, 1082
Tru9I TTAA 5 cut(s) 153, 297, 813, 917, 1082
TscAI CASTG 2 cut(s) 714, 890
TseI GCWGC 3 cut(s) 191, 827, 1026
TspDTI ATGAA 3 cut(s) 50, 199, 547
TspGWI ACGGA 1 cut(s) 1049
TspRI CASTG 2 cut(s) 714, 890
Van91I CCANNNNNTGG 1 cut(s) 436
VneI GTGCAC 1 cut(s) 591
VpaK11BI GGWCC 3 cut(s) 317, 836, 898
XagI CCTNNNNNAGG 1 cut(s) 837
XceI RCATGY 2 cut(s) 419, 1089
XhoI CTCGAG 1 cut(s) 1090
XmiI GTMKAC 1 cut(s) 78
XspI CTAG 3 cut(s) 314, 476, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.