pycom04g13880

Domain of unknown function (DUF3453)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007393
Physical Location & Seq
Forward (+)
16765725 .. 16770460
4736 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1353 bp
ATGGTGGGCATGACGATGACCGCCAATTCCCACGAAAGGCTCGCTTATCTCATGGACTCCGCCATCTTCGTCTCCGACATTCCGTCGAAGCTCGACCGGTTGCCCCAATCGAAGCACGATTTGGTCCGCCAACAGGACCCTGATTTGCTTTCCGAACTCCTCACTCGCTTCTTCGAGCTTCAGTCCGACCAGTTCGGCCCGGTTCGTAAATTCGCCACTGAGTGCGTTTTTGGTCTACATACTAGTGAATTGGACAGTTTGCTTGAGTTGGCTTGGGCATGGGTGTTAAAGTTGAAGGATGAAATATACTCTATAGCTTTTCAGCTGGGAAGTGGGGGAGTAAGGTTGCGAGCACTGAAATTTGTTGAGTCAGTCATTCTTCTTTATACTCCTGACCCTAATGGCTCCCCGGCGCCCCCTGCTCATGAAGGGGACTTGGTGGATTTTAATGTATCCTGGCTTCATGGTGGTCATCCCCTACTCAATGTTGGAGATTTGTCAATTGAGGCTAGTAAAAGTTTGGGTTTATTGCTTGATCGACTCAGGTTCCCAACTGTGAAATCGCTTGGTAGCTTAGCGATCGTTGTGCTTATTAACAGTCTTTCAGCAATTGCAAAGAAGAGGCCTGCATTCTATGGTCGTATTTTGCCTGTATTACTTGGATTTGATCCCTCAAGCACTCTCATTAATGGGGTACATGTTTCTGGGCCACGTTATGCCTTAAAAAATGCCTTTCTCACCTGCTTAAAGTGTACACACCAGGGTGCTGCACTGTGGCGGGATTGTTTGGTTGGTGCGATGAGAAAGATGCAAGCTGGAGGGCTGGTAGAGCTAGCCACCCAGCAAGAATGCAAGATTAATGGAAGTGTGGAAGATGGGCTAGATGATTCTCTAGTTACTAAGGTAAAAAAAAAAAAAAAAAAAATGGATGCTCTTGCACAAGTGCACGTTGGGCCATGGGCATCATGCATCTTGAAGCTTCTTGAGCATGAGCTTTATGTTTTGCAGGAAGAGAAGCCCACTATTAACATATCTAATGCTGTGCAAAGTAGTTTCGGGAAGAAACGGTTAGGAGCACTTGATAGTAGTTCGATCTTTGTCTCTGGGGATTCGGCTAGAGAGTTAGATAGGAATGTTTCTGTGTCTCAAGATGATGTCTCTTCAAGTGGAACTACCACTTCTAGAGGAGATGGTGATAGTGGACCTGTACAGCAACTTATTGCTATGTTTGGTGCGCTGGTTGCTCAAGGTGAAAAAGCTGTTGGTTCTTTGGAAATTCTTATCTCAAGTATCTCTGGTGACTTGTTAGCAGAGGTCGTGATGGCTAATATGTGCAACCTTCCTCGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04530 map03015 map04530
Pfam Domains
Protein Families
DUF3453 Symplekin_C

Protein Analysis

451

Amino Acids

48.81

Weight (kDa)

6.38

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYMPK_PTA1_N PF11935 91 - 253 5.9e-30 Symplekin/PTA1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 749
Acc36I ACCTGC 1 cut(s) 749
AccB1I GGYRCC 1 cut(s) 412
AccI GTMKAC 1 cut(s) 235
AciI CCGC 5 cut(s) 21, 60, 127, 778, 1348
AclWI GGATC 1 cut(s) 662
AcsI RAATTY 3 cut(s) 209, 359, 1275
AcuI CTGAAG 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 413
AdeI CACNNNGTG 1 cut(s) 222
AfaI GTAC 3 cut(s) 696, 754, 1209
AfiI CCNNNNNNNGG 2 cut(s) 36, 133
AflIII ACRYGT 1 cut(s) 697
AgeI ACCGGT 1 cut(s) 96
AgsI TTSAA 3 cut(s) 295, 976, 1164
AhdI GACNNNNNGTC 1 cut(s) 82
AhlI ACTAGT 1 cut(s) 242
AjnI CCWGG 2 cut(s) 455, 759
AjuI GAANNNNNNNTTGG 4 cut(s) 104, 136, 1245, 1277
AleI CACNNNNGTG 1 cut(s) 762
Alw21I GWGCWC 3 cut(s) 355, 948, 1078
Alw26I GTCTC 4 cut(s) 76, 1105, 1149, 1162
Alw44I GTGCAC 1 cut(s) 944
AlwI GGATC 1 cut(s) 662
AoxI GGCC 4 cut(s) 196, 623, 707, 953
ApaLI GTGCAC 1 cut(s) 944
ApeKI GCWGC 1 cut(s) 767
ApoI RAATTY 3 cut(s) 209, 359, 1275
AseI ATTAAT 2 cut(s) 687, 858
AsiGI ACCGGT 1 cut(s) 96
AspLEI GCGC 2 cut(s) 415, 1237
AspS9I GGNCC 6 cut(s) 124, 136, 197, 707, 953, 1202
AsuC2I CCSGG 2 cut(s) 200, 410
AsuHPI GGTGA 4 cut(s) 730, 1205, 1262, 1310
AsuNHI GCTAGC 1 cut(s) 832
AvaII GGWCC 3 cut(s) 124, 136, 1202
BaeGI GKGCMC 1 cut(s) 948
BanI GGYRCC 1 cut(s) 412
BarI GAAGNNNNNNTAC 2 cut(s) 444, 476
Bbv12I GWGCWC 3 cut(s) 355, 948, 1078
BbvI GCAGC 1 cut(s) 754
BccI CCATC 4 cut(s) 71, 869, 1184, 1315
BcgI CGANNNNNNTGC 2 cut(s) 568, 602
BciT130I CCWGG 2 cut(s) 457, 761
BciVI GTATCC 1 cut(s) 463
BcnI CCSGG 2 cut(s) 200, 410
BcoDI GTCTC 4 cut(s) 76, 1105, 1149, 1162
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 7 cut(s) 243, 510, 833, 881, 893, 1116, 1182
BfmI CTRYAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 416
BfuAI ACCTGC 1 cut(s) 749
BfuI GTATCC 1 cut(s) 463
BisI GCNGC 1 cut(s) 768
BlpI GCTNAGC 1 cut(s) 574
BlsI GCNGC 1 cut(s) 769
Bme1390I CCNGG 4 cut(s) 200, 410, 457, 761
Bme18I GGWCC 3 cut(s) 124, 136, 1202
BmeRI GACNNNNNGTC 1 cut(s) 82
BmgT120I GGNCC 6 cut(s) 124, 136, 197, 707, 953, 1202
BmiI GGNNCC 4 cut(s) 138, 406, 414, 548
BmrFI CCNGG 4 cut(s) 200, 410, 457, 761
BmsI GCATC 4 cut(s) 798, 919, 971, 978
BmtI GCTAGC 1 cut(s) 836
BpmI CTGGAG 1 cut(s) 837
Bpu1102I GCTNAGC 1 cut(s) 574
BpuEI CTTGAG 6 cut(s) 284, 658, 1004, 1131, 1230, 1270
BpuMI CCSGG 2 cut(s) 200, 410
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 4 cut(s) 408, 760, 956, 1343
BsaWI WCCGGW 1 cut(s) 96
BsaXI ACNNNNNCTCC 2 cut(s) 810, 840
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 133
Bse118I RCCGGY 1 cut(s) 96
Bse1I ACTGG 1 cut(s) 190
BseBI CCWGG 2 cut(s) 457, 761
BseDI CCNNGG 4 cut(s) 408, 760, 956, 1343
BseGI GGATG 3 cut(s) 304, 472, 934
BseLI CCNNNNNNNGG 2 cut(s) 36, 133
BseMII CTCAG 2 cut(s) 210, 556
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 2 cut(s) 149, 1200
BseSI GKGCMC 1 cut(s) 948
BseXI GCAGC 1 cut(s) 754
BseYI CCCAGC 2 cut(s) 325, 840
BsgI GTGCAG 1 cut(s) 753
Bsh1285I CGRYCG 2 cut(s) 97, 582
BshFI GGCC 4 cut(s) 198, 625, 709, 955
BshNI GGYRCC 1 cut(s) 412
BshTI ACCGGT 1 cut(s) 96
BsiEI CGRYCG 2 cut(s) 97, 582
BsiHKAI GWGCWC 3 cut(s) 355, 948, 1078
BsiSI CCGG 3 cut(s) 97, 200, 410
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 2 cut(s) 36, 133
BsmAI GTCTC 4 cut(s) 76, 1105, 1149, 1162
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 446
BsmI GAATGC 2 cut(s) 629, 854
BsnI GGCC 4 cut(s) 198, 625, 709, 955
Bsp1286I GDGCHC 3 cut(s) 355, 948, 1078
Bsp1407I TGTACA 2 cut(s) 752, 1207
Bsp143I GATC 4 cut(s) 535, 579, 667, 1092
Bsp1720I GCTNAGC 1 cut(s) 574
Bsp19I CCATGG 1 cut(s) 956
BspACI CCGC 5 cut(s) 21, 60, 127, 778, 1348
BspANI GGCC 4 cut(s) 198, 625, 709, 955
BspCNI CTCAG 2 cut(s) 211, 555
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 4 cut(s) 138, 406, 414, 548
BspMI ACCTGC 1 cut(s) 749
BspOI GCTAGC 1 cut(s) 836
BspPI GGATC 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 412
BsrFI RCCGGY 1 cut(s) 96
BsrGI TGTACA 2 cut(s) 752, 1207
BsrI ACTGG 1 cut(s) 190
BssAI RCCGGY 1 cut(s) 96
BssECI CCNNGG 4 cut(s) 408, 760, 956, 1343
BssMI GATC 4 cut(s) 535, 579, 667, 1092
BssNI GRCGYC 1 cut(s) 413
BssT1I CCWWGG 1 cut(s) 956
Bst2UI CCWGG 2 cut(s) 457, 761
Bst4CI ACNGT 5 cut(s) 257, 556, 599, 774, 1068
Bst6I CTCTTC 3 cut(s) 614, 1005, 1165
BstACI GRCGYC 1 cut(s) 413
BstAUI TGTACA 2 cut(s) 752, 1207
BstC8I GCNNGC 5 cut(s) 42, 351, 627, 813, 834
BstDEI CTNAG 4 cut(s) 219, 542, 574, 900
BstDSI CCRYGG 1 cut(s) 956
BstF5I GGATG 3 cut(s) 304, 472, 934
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 2 cut(s) 415, 1237
BstKTI GATC 4 cut(s) 538, 582, 670, 1095
BstMAI GTCTC 4 cut(s) 76, 1105, 1149, 1162
BstMBI GATC 4 cut(s) 535, 579, 667, 1092
BstMCI CGRYCG 2 cut(s) 97, 582
BstMWI GCNNNNNNNGC 5 cut(s) 419, 829, 952, 985, 1241
BstNI CCWGG 2 cut(s) 457, 761
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 4 cut(s) 198, 408, 455, 759
BstSFI CTRYAG 1 cut(s) 312
BstSLI GKGCMC 1 cut(s) 948
BstV1I GCAGC 1 cut(s) 754
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 4 cut(s) 198, 625, 709, 955
BtgI CCRYGG 1 cut(s) 956
BtgZI GCGATG 1 cut(s) 812
BtsCI GGATG 3 cut(s) 304, 472, 934
BtsIMutI CAGTG 3 cut(s) 216, 353, 770
BveI ACCTGC 1 cut(s) 749
Cac8I GCNNGC 5 cut(s) 42, 351, 627, 813, 834
CciI TCATGA 1 cut(s) 424
CfoI GCGC 2 cut(s) 415, 1237
Cfr10I RCCGGY 1 cut(s) 96
Cfr13I GGNCC 6 cut(s) 124, 136, 197, 707, 953, 1202
Csp6I GTAC 3 cut(s) 695, 753, 1208
CspAI ACCGGT 1 cut(s) 96
CviAII CATG 9 cut(s) 10, 52, 279, 425, 464, 698, 957, 966, 989
CviQI GTAC 3 cut(s) 695, 753, 1208
DdeI CTNAG 4 cut(s) 219, 542, 574, 900
DinI GGCGCC 1 cut(s) 414
DpnI GATC 4 cut(s) 537, 581, 669, 1094
DpnII GATC 4 cut(s) 535, 579, 667, 1092
DraIII CACNNNGTG 1 cut(s) 222
DriI GACNNNNNGTC 1 cut(s) 82
Eam1104I CTCTTC 3 cut(s) 614, 1005, 1165
Eam1105I GACNNNNNGTC 1 cut(s) 82
EarI CTCTTC 3 cut(s) 614, 1005, 1165
EciI GGCGGA 2 cut(s) 49, 116
Eco130I CCWWGG 1 cut(s) 956
Eco147I AGGCCT 1 cut(s) 625
Eco47I GGWCC 3 cut(s) 124, 136, 1202
Eco57I CTGAAG 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 455, 759
EcoT14I CCWWGG 1 cut(s) 956
EcoT22I ATGCAT 1 cut(s) 971
EgeI GGCGCC 1 cut(s) 414
EheI GGCGCC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 956
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 9 cut(s) 13, 55, 282, 428, 467, 701, 960, 969, 992
FalI AAGNNNNNCTT 2 cut(s) 28, 60
FaqI GGGAC 1 cut(s) 446
FatI CATG 9 cut(s) 9, 51, 278, 424, 463, 697, 956, 965, 988
FauI CCCGC 1 cut(s) 771
FblI GTMKAC 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 768
FokI GGATG 3 cut(s) 311, 459, 941
Fsp4HI GCNGC 1 cut(s) 768
FspBI CTAG 7 cut(s) 243, 510, 833, 881, 893, 1116, 1182
GlaI GCGC 2 cut(s) 414, 1236
GluI GCNGC 1 cut(s) 768
GsaI CCCAGC 2 cut(s) 329, 844
GsuI CTGGAG 1 cut(s) 837
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 4 cut(s) 198, 625, 709, 955
HapII CCGG 3 cut(s) 97, 200, 410
HhaI GCGC 2 cut(s) 415, 1237
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 9 cut(s) 13, 55, 282, 428, 467, 701, 960, 969, 992
Hin6I GCGC 2 cut(s) 413, 1235
HinP1I GCGC 2 cut(s) 413, 1235
HindIII AAGCTT 1 cut(s) 977
HinfI GANTC 5 cut(s) 56, 368, 540, 887, 1109
HpaII CCGG 3 cut(s) 97, 200, 410
HphI GGTGA 4 cut(s) 730, 1205, 1262, 1310
Hpy166II GTNNAC 5 cut(s) 236, 753, 755, 946, 1202
Hpy188I TCNGA 3 cut(s) 76, 154, 187
Hpy188III TCNNGA 8 cut(s) 392, 425, 973, 983, 1057, 1148, 1182, 1318
Hpy8I GTNNAC 5 cut(s) 236, 753, 755, 946, 1202
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 3 cut(s) 289, 422, 1349
HpyCH4III ACNGT 5 cut(s) 257, 556, 599, 774, 1068
HpyCH4IV ACGT 2 cut(s) 712, 948
HpyF10VI GCNNNNNNNGC 5 cut(s) 419, 829, 952, 985, 1241
HpyF3I CTNAG 4 cut(s) 219, 542, 574, 900
HpySE526I ACGT 2 cut(s) 712, 948
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 9 cut(s) 13, 55, 282, 428, 467, 701, 960, 969, 992
HspAI GCGC 2 cut(s) 413, 1235
KasI GGCGCC 1 cut(s) 412
Kzo9I GATC 4 cut(s) 535, 579, 667, 1092
LmnI GCTCC 2 cut(s) 410, 1073
Lsp1109I GCAGC 1 cut(s) 754
LweI GCATC 4 cut(s) 798, 919, 971, 978
MaeI CTAG 7 cut(s) 243, 510, 833, 881, 893, 1116, 1182
MaeII ACGT 2 cut(s) 712, 948
MaeIII GTNAC 2 cut(s) 895, 1298
MalI GATC 4 cut(s) 537, 581, 669, 1094
MboI GATC 4 cut(s) 535, 579, 667, 1092
MboII GAAGA 8 cut(s) 58, 163, 371, 631, 884, 1022, 1072, 1152
MfeI CAATTG 2 cut(s) 501, 609
MhlI GDGCHC 3 cut(s) 355, 948, 1078
MluCI AATT 7 cut(s) 25, 209, 248, 359, 501, 609, 1275
Mly113I GGCGCC 1 cut(s) 413
MlyI GAGTC 3 cut(s) 50, 377, 534
MmeI TCCRAC 3 cut(s) 99, 210, 469
MnlI CCTC 8 cut(s) 170, 499, 615, 682, 812, 1178, 1306, 1353
Mph1103I ATGCAT 1 cut(s) 971
MseI TTAA 8 cut(s) 287, 447, 594, 687, 722, 746, 858, 1026
MslI CAYNNNNRTG 3 cut(s) 14, 243, 762
MspA1I CMGCKG 1 cut(s) 325
MspI CCGG 3 cut(s) 97, 200, 410
MspR9I CCNGG 4 cut(s) 200, 410, 457, 761
MunI CAATTG 2 cut(s) 501, 609
Mva1269I GAATGC 2 cut(s) 629, 854
MvaI CCWGG 2 cut(s) 457, 761
MwoI GCNNNNNNNGC 5 cut(s) 419, 829, 952, 985, 1241
NarI GGCGCC 1 cut(s) 413
NciI CCSGG 2 cut(s) 200, 410
NcoI CCATGG 1 cut(s) 956
NdeII GATC 4 cut(s) 535, 579, 667, 1092
NheI GCTAGC 1 cut(s) 832
NlaIII CATG 9 cut(s) 13, 55, 282, 428, 467, 701, 960, 969, 992
NlaIV GGNNCC 4 cut(s) 138, 406, 414, 548
NmeAIII GCCGAG 1 cut(s) 1324
NmuCI GTSAC 1 cut(s) 1298
NsiI ATGCAT 1 cut(s) 971
NspI RCATGY 1 cut(s) 701
OliI CACNNNNGTG 1 cut(s) 762
PagI TCATGA 1 cut(s) 424
PaqCI CACCTGC 1 cut(s) 749
PceI AGGCCT 1 cut(s) 625
PciI ACATGT 1 cut(s) 697
PctI GAATGC 2 cut(s) 629, 854
PfeI GAWTC 2 cut(s) 887, 1109
PinAI ACCGGT 1 cut(s) 96
PkrI GCNGC 1 cut(s) 769
Ple19I CGATCG 1 cut(s) 582
PleI GAGTC 3 cut(s) 50, 376, 534
PluTI GGCGCC 1 cut(s) 416
PpsI GAGTC 3 cut(s) 50, 376, 534
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 697
PshBI ATTAAT 2 cut(s) 687, 858
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 455, 759
PspFI CCCAGC 2 cut(s) 325, 840
PspGI CCWGG 2 cut(s) 455, 759
PspN4I GGNNCC 4 cut(s) 138, 406, 414, 548
PspPI GGNCC 6 cut(s) 124, 136, 197, 707, 953, 1202
PspPPI RGGWCCY 1 cut(s) 136
PvuI CGATCG 1 cut(s) 582
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 3 cut(s) 696, 754, 1209
RsaNI GTAC 3 cut(s) 695, 753, 1208
RseI CAYNNNNRTG 3 cut(s) 14, 243, 762
SaqAI TTAA 8 cut(s) 287, 447, 594, 687, 722, 746, 858, 1026
SatI GCNGC 1 cut(s) 768
Sau3AI GATC 4 cut(s) 535, 579, 667, 1092
Sau96I GGNCC 6 cut(s) 124, 136, 197, 707, 953, 1202
SchI GAGTC 3 cut(s) 50, 377, 534
ScrFI CCNGG 4 cut(s) 200, 410, 457, 761
SduI GDGCHC 3 cut(s) 355, 948, 1078
SfaNI GCATC 4 cut(s) 798, 919, 971, 978
SfcI CTRYAG 1 cut(s) 312
SfoI GGCGCC 1 cut(s) 414
SinI GGWCC 3 cut(s) 124, 136, 1202
SmiMI CAYNNNNRTG 3 cut(s) 14, 243, 762
SmlI CTYRAG 6 cut(s) 263, 673, 983, 1146, 1245, 1285
SmoI CTYRAG 6 cut(s) 263, 673, 983, 1146, 1245, 1285
SpeI ACTAGT 1 cut(s) 242
Sse9I AATT 7 cut(s) 25, 209, 248, 359, 501, 609, 1275
SseBI AGGCCT 1 cut(s) 625
SsiI CCGC 5 cut(s) 21, 60, 127, 778, 1348
SspDI GGCGCC 1 cut(s) 412
SspMI CTAG 7 cut(s) 243, 510, 833, 881, 893, 1116, 1182
StuI AGGCCT 1 cut(s) 625
StyD4I CCNGG 4 cut(s) 198, 408, 455, 759
StyI CCWWGG 1 cut(s) 956
TaaI ACNGT 5 cut(s) 257, 556, 599, 774, 1068
TaiI ACGT 2 cut(s) 715, 951
TaqI TCGA 6 cut(s) 86, 93, 110, 174, 538, 1091
TasI AATT 7 cut(s) 25, 209, 248, 359, 501, 609, 1275
TatI WGTACW 2 cut(s) 752, 1207
TfiI GAWTC 2 cut(s) 887, 1109
Tru1I TTAA 8 cut(s) 287, 447, 594, 687, 722, 746, 858, 1026
Tru9I TTAA 8 cut(s) 287, 447, 594, 687, 722, 746, 858, 1026
TscAI CASTG 3 cut(s) 223, 360, 777
TseFI GTSAC 1 cut(s) 1298
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 1 cut(s) 1298
TspDTI ATGAA 3 cut(s) 315, 441, 452
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 3 cut(s) 223, 360, 777
VneI GTGCAC 1 cut(s) 944
VpaK11BI GGWCC 3 cut(s) 124, 136, 1202
VspI ATTAAT 2 cut(s) 687, 858
XapI RAATTY 3 cut(s) 209, 359, 1275
XbaI TCTAGA 1 cut(s) 1181
XceI RCATGY 1 cut(s) 701
XmiI GTMKAC 1 cut(s) 235
XspI CTAG 7 cut(s) 243, 510, 833, 881, 893, 1116, 1182
Zsp2I ATGCAT 1 cut(s) 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.