pycom04g14270

Chlorophyll synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010287
Physical Location & Seq
Forward (+)
17150202 .. 17153770
3569 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1125 bp
ATGGCGTCCGTACTCAATATGGTGTCGTCTATTAGCCTATCGAACCTCAACGCCGACCGAGTTCGGACTCAGTCCGCCTTCGCGCCGGTTTCGGTTTCGTTTACCAGAAGGAGGCTTACGGTTAGGGCTGCGGAGACCGATACCAACGAAGCAGTTGAATCTTCAGTGCCGGATAAGGCACCGGCTAAAGCCGGTTCGAGCGTCAATCAGCTTCTTGGGATTAAAGGAGCTTCTCAAGAAACTAACAAGTGGAAGATTCGTCTTCAGCTGACAAAGCCTGTAACTTGGCCTCCACTGGTATGGGGAGTAGTTTGCGGAGCTGCTGCTTCTGGAAATTTTCATTGGACTTTTGAGGATGTTGCTAAATCAATAATGTGCATGTGTATGTCTGGCCCTTTACTCACTGGCTATACCCAGACACTAAATGACTGGTATGACCGAGAGATTGATGCAATTAATGAACCTTATCGTCCAATTCCCTCAGGGGCAATATCTGAGAATGAGGTTATTACTCAAATCTGGGTGCTGCTTTTAGGAGGCCTTAGTTTTGCTGGCATATTAGATGTGTGGGCAGGACATAAATTCCCAATATTATTTTACCTTGCTGTGGGTGGATCCTTGCTATCGTACATATATTCCGCTCCACCTTTAAAGCTGAAACAAAACGGATGGATTGGAAATTTTGCCCTGGGTGCAAGTTATATCAGCTTGCCATGGTGGGCTGGTCAGGCATTATTTGGGACCCTTACGCCCGACATAATCGTCCTCACACTCTTGTACAGCATAGCTGGGTTGGGAATTGCTATTGTAAACGACTTTAAAAGTGTTGAAGGAGATAGAGCAATGGGGCTTCAGTCACTCCCTGTAGCTTTTGGTTCTGAAACTGCCAAATGGATTTGTGTTGGCGCCATTGACATCACTCAGTTATCGGTCGCTGGTTATCTGCTATGGGCTGGTAAACCATATTATGCCTTAGCTCTCCTTGCTTTGATAGCTCCCCAAGTCTTTTTCCAGTTCAAGTACTTTCTCAAAGACCCTGTCAAGTACGATGTTAAATATCAGGCAAGTGCGCAGCCGTTTCTTGTGCTCGGTCTTTTAGTAACAGCGTTGGCGACAAGTCATTAA

Protein Analysis

375

Amino Acids

40.58

Weight (kDa)

8.22

Isoelectric Point (pI)

27.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 97 - 346 2e-38 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 761
Acc16I TGCGCA 1 cut(s) 1071
AccB1I GGYRCC 2 cut(s) 178, 905
AccBSI CCGCTC 1 cut(s) 641
AccII CGCG 1 cut(s) 83
AciI CCGC 4 cut(s) 75, 131, 315, 639
AclWI GGATC 2 cut(s) 609, 622
AcsI RAATTY 3 cut(s) 334, 581, 679
AcuI CTGAAG 3 cut(s) 147, 248, 836
AcyI GRCGYC 2 cut(s) 5, 906
AfaI GTAC 5 cut(s) 12, 629, 779, 1022, 1046
AfiI CCNNNNNNNGG 2 cut(s) 111, 607
AgsI TTSAA 3 cut(s) 158, 830, 1018
AjnI CCWGG 1 cut(s) 687
AjuI GAANNNNNNNTTGG 2 cut(s) 325, 357
Alw21I GWGCWC 1 cut(s) 1089
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 2 cut(s) 609, 622
AoxI GGCC 3 cut(s) 287, 391, 538
ApeKI GCWGC 5 cut(s) 128, 320, 323, 526, 1072
ApoI RAATTY 3 cut(s) 334, 581, 679
ArsI GACNNNNNNTTYG 2 cut(s) 1023, 1055
AseI ATTAAT 1 cut(s) 456
AspLEI GCGC 3 cut(s) 85, 908, 1072
AspS9I GGNCC 2 cut(s) 392, 741
AvaII GGWCC 1 cut(s) 741
AxyI CCTNAGG 1 cut(s) 481
BamHI GGATCC 1 cut(s) 614
BanI GGYRCC 2 cut(s) 178, 905
BarI GAAGNNNNNNTAC 2 cut(s) 100, 132
BbsI GAAGAC 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 1089
BbvI GCAGC 5 cut(s) 115, 307, 310, 513, 1084
BccI CCATC 1 cut(s) 663
BceAI ACGGC 1 cut(s) 1060
BciT130I CCWGG 1 cut(s) 689
BcoDI GTCTC 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 864
BfoI RGCGCY 1 cut(s) 909
BisI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
BlsI GCNGC 5 cut(s) 130, 322, 325, 528, 1074
BmcAI AGTACT 1 cut(s) 1022
Bme1390I CCNGG 1 cut(s) 689
Bme18I GGWCC 1 cut(s) 741
BmgT120I GGNCC 2 cut(s) 392, 741
BmiI GGNNCC 5 cut(s) 180, 616, 742, 743, 907
BmrFI CCNGG 1 cut(s) 689
BmsI GCATC 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 254
Bpu10I CCTNAGC 1 cut(s) 973
BpuEI CTTGAG 1 cut(s) 219
BsaHI GRCGYC 2 cut(s) 5, 906
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 687, 688, 713
BsaXI ACNNNNNCTCC 2 cut(s) 274, 304
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 607
Bse118I RCCGGY 3 cut(s) 85, 181, 191
Bse1I ACTGG 4 cut(s) 300, 409, 434, 1012
Bse21I CCTNAGG 1 cut(s) 481
Bse3DI GCAATG 1 cut(s) 849
BseBI CCWGG 1 cut(s) 689
BseDI CCNNGG 3 cut(s) 687, 688, 713
BseGI GGATG 2 cut(s) 361, 674
BseLI CCNNNNNNNGG 2 cut(s) 111, 607
BseMI GCAATG 1 cut(s) 849
BseMII CTCAG 4 cut(s) 83, 486, 495, 935
BseNI ACTGG 4 cut(s) 300, 409, 434, 1012
BseXI GCAGC 5 cut(s) 115, 307, 310, 513, 1084
BseYI CCCAGC 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 83
Bsh1285I CGRYCG 2 cut(s) 58, 933
BshFI GGCC 3 cut(s) 289, 393, 540
BshNI GGYRCC 2 cut(s) 178, 905
BsiEI CGRYCG 2 cut(s) 58, 933
BsiHKAI GWGCWC 1 cut(s) 1089
BsiSI CCGG 4 cut(s) 86, 170, 182, 192
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 2 cut(s) 111, 607
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 754
BsnI GGCC 3 cut(s) 289, 393, 540
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 1089
Bsp1407I TGTACA 1 cut(s) 777
Bsp143I GATC 1 cut(s) 614
Bsp19I CCATGG 1 cut(s) 713
BspACI CCGC 4 cut(s) 75, 131, 315, 639
BspANI GGCC 3 cut(s) 289, 393, 540
BspCNI CTCAG 4 cut(s) 82, 487, 494, 934
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 5 cut(s) 180, 616, 742, 743, 907
BspPI GGATC 2 cut(s) 609, 622
BspT107I GGYRCC 2 cut(s) 178, 905
BspTNI GGTCTC 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 641
BsrDI GCAATG 1 cut(s) 849
BsrFI RCCGGY 3 cut(s) 85, 181, 191
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 4 cut(s) 300, 409, 434, 1012
BssAI RCCGGY 3 cut(s) 85, 181, 191
BssECI CCNNGG 3 cut(s) 687, 688, 713
BssMI GATC 1 cut(s) 614
BssNI GRCGYC 2 cut(s) 5, 906
BssT1I CCWWGG 1 cut(s) 713
Bst2UI CCWGG 1 cut(s) 689
Bst4CI ACNGT 1 cut(s) 121
BstACI GRCGYC 2 cut(s) 5, 906
BstAUI TGTACA 1 cut(s) 777
BstC8I GCNNGC 2 cut(s) 553, 710
BstDEI CTNAG 6 cut(s) 69, 481, 495, 542, 921, 973
BstDSI CCRYGG 1 cut(s) 713
BstF5I GGATG 2 cut(s) 361, 674
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 909
BstHHI GCGC 3 cut(s) 85, 908, 1072
BstKTI GATC 1 cut(s) 617
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 1 cut(s) 614
BstMCI CGRYCG 2 cut(s) 58, 933
BstMWI GCNNNNNNNGC 5 cut(s) 274, 692, 728, 983, 992
BstNI CCWGG 1 cut(s) 689
BstNSI RCATGY 1 cut(s) 382
BstSCI CCNGG 1 cut(s) 687
BstSFI CTRYAG 1 cut(s) 864
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 5 cut(s) 115, 307, 310, 513, 1084
BstV2I GAAGAC 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 614
BstXI CCANNNNNNTGG 1 cut(s) 300
BstYI RGATCY 1 cut(s) 614
Bsu36I CCTNAGG 1 cut(s) 481
BsuRI GGCC 3 cut(s) 289, 393, 540
BtgI CCRYGG 1 cut(s) 713
BtsCI GGATG 2 cut(s) 361, 674
BtsIMutI CAGTG 3 cut(s) 171, 293, 402
Cac8I GCNNGC 2 cut(s) 553, 710
CfoI GCGC 3 cut(s) 85, 908, 1072
Cfr10I RCCGGY 3 cut(s) 85, 181, 191
Cfr13I GGNCC 2 cut(s) 392, 741
CseI GACGC 1 cut(s) 190
Csp6I GTAC 5 cut(s) 11, 628, 778, 1021, 1045
CviAII CATG 2 cut(s) 379, 714
CviQI GTAC 5 cut(s) 11, 628, 778, 1021, 1045
DdeI CTNAG 6 cut(s) 69, 481, 495, 542, 921, 973
DinI GGCGCC 1 cut(s) 907
DpnI GATC 1 cut(s) 616
DpnII GATC 1 cut(s) 614
DraI TTTAAA 2 cut(s) 651, 820
DrdI GACNNNNNNGTC 1 cut(s) 761
DseDI GACNNNNNNGTC 1 cut(s) 761
EciI GGCGGA 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 713
Eco147I AGGCCT 1 cut(s) 540
Eco31I GGTCTC 1 cut(s) 128
Eco47I GGWCC 1 cut(s) 741
Eco57I CTGAAG 3 cut(s) 147, 248, 836
Eco81I CCTNAGG 1 cut(s) 481
EcoO109I RGGNCCY 1 cut(s) 741
EcoRII CCWGG 1 cut(s) 687
EcoT14I CCWWGG 1 cut(s) 713
EgeI GGCGCC 1 cut(s) 907
EheI GGCGCC 1 cut(s) 907
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 2 cut(s) 382, 717
FaqI GGGAC 1 cut(s) 754
FatI CATG 2 cut(s) 378, 713
Fnu4HI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
FokI GGATG 2 cut(s) 368, 681
Fsp4HI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
FspI TGCGCA 1 cut(s) 1071
GlaI GCGC 3 cut(s) 84, 907, 1071
GluI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
GsaI CCCAGC 1 cut(s) 792
HaeII RGCGCY 1 cut(s) 909
HaeIII GGCC 3 cut(s) 289, 393, 540
HapII CCGG 4 cut(s) 86, 170, 182, 192
HgaI GACGC 1 cut(s) 190
HhaI GCGC 3 cut(s) 85, 908, 1072
Hin1I GRCGYC 2 cut(s) 5, 906
Hin1II CATG 2 cut(s) 382, 717
Hin6I GCGC 3 cut(s) 83, 906, 1070
HinP1I GCGC 3 cut(s) 83, 906, 1070
HinfI GANTC 3 cut(s) 67, 158, 256
HpaII CCGG 4 cut(s) 86, 170, 182, 192
Hpy166II GTNNAC 3 cut(s) 102, 811, 959
Hpy188I TCNGA 3 cut(s) 66, 496, 880
Hpy188III TCNNGA 2 cut(s) 236, 330
Hpy8I GTNNAC 3 cut(s) 102, 811, 959
HpyAV CCTTC 3 cut(s) 88, 102, 824
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4V TGCA 3 cut(s) 378, 452, 695
HpyF10VI GCNNNNNNNGC 5 cut(s) 274, 692, 728, 983, 992
HpyF3I CTNAG 6 cut(s) 69, 481, 495, 542, 921, 973
Hsp92I GRCGYC 2 cut(s) 5, 906
Hsp92II CATG 2 cut(s) 382, 717
HspAI GCGC 3 cut(s) 83, 906, 1070
KasI GGCGCC 1 cut(s) 905
KflI GGGWCCC 1 cut(s) 741
Kzo9I GATC 1 cut(s) 614
LmnI GCTCC 4 cut(s) 227, 317, 646, 1000
Lsp1109I GCAGC 5 cut(s) 115, 307, 310, 513, 1084
LweI GCATC 1 cut(s) 439
MaeIII GTNAC 3 cut(s) 280, 855, 1099
MalI GATC 1 cut(s) 616
MbiI CCGCTC 1 cut(s) 641
MboI GATC 1 cut(s) 614
MboII GAAGA 3 cut(s) 153, 254, 265
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 1 cut(s) 1089
MluCI AATT 6 cut(s) 334, 453, 474, 581, 679, 798
Mly113I GGCGCC 1 cut(s) 906
MlyI GAGTC 1 cut(s) 61
MnlI CCTC 8 cut(s) 56, 105, 300, 346, 490, 496, 530, 776
MseI TTAA 6 cut(s) 222, 456, 650, 819, 1053, 1123
MslI CAYNNNNRTG 2 cut(s) 298, 383
MspA1I CMGCKG 1 cut(s) 268
MspI CCGG 4 cut(s) 86, 170, 182, 192
MspR9I CCNGG 1 cut(s) 689
MvaI CCWGG 1 cut(s) 689
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 5 cut(s) 274, 692, 728, 983, 992
NarI GGCGCC 1 cut(s) 906
NcoI CCATGG 1 cut(s) 713
NdeII GATC 1 cut(s) 614
NlaIII CATG 2 cut(s) 382, 717
NlaIV GGNNCC 5 cut(s) 180, 616, 742, 743, 907
NmuCI GTSAC 1 cut(s) 855
NsbI TGCGCA 1 cut(s) 1071
NspI RCATGY 1 cut(s) 382
PasI CCCWGGG 1 cut(s) 688
PceI AGGCCT 1 cut(s) 540
PfeI GAWTC 2 cut(s) 158, 256
PflFI GACNNNGTC 2 cut(s) 70, 1037
PkrI GCNGC 5 cut(s) 130, 322, 325, 528, 1074
PleI GAGTC 1 cut(s) 61
PluTI GGCGCC 1 cut(s) 909
PpsI GAGTC 1 cut(s) 61
PpuMI RGGWCCY 1 cut(s) 741
PshBI ATTAAT 1 cut(s) 456
Psp5II RGGWCCY 1 cut(s) 741
Psp6I CCWGG 1 cut(s) 687
PspFI CCCAGC 1 cut(s) 788
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 5 cut(s) 180, 616, 742, 743, 907
PspPI GGNCC 2 cut(s) 392, 741
PspPPI RGGWCCY 1 cut(s) 741
PsuI RGATCY 1 cut(s) 614
PsyI GACNNNGTC 2 cut(s) 70, 1037
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 5 cut(s) 12, 629, 779, 1022, 1046
RsaNI GTAC 5 cut(s) 11, 628, 778, 1021, 1045
RseI CAYNNNNRTG 2 cut(s) 298, 383
SaqAI TTAA 6 cut(s) 222, 456, 650, 819, 1053, 1123
SatI GCNGC 5 cut(s) 129, 321, 324, 527, 1073
Sau3AI GATC 1 cut(s) 614
Sau96I GGNCC 2 cut(s) 392, 741
ScaI AGTACT 1 cut(s) 1022
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 1 cut(s) 689
SduI GDGCHC 1 cut(s) 1089
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 864
SfoI GGCGCC 1 cut(s) 907
SinI GGWCC 1 cut(s) 741
SmiMI CAYNNNNRTG 2 cut(s) 298, 383
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
Sse9I AATT 6 cut(s) 334, 453, 474, 581, 679, 798
SseBI AGGCCT 1 cut(s) 540
SsiI CCGC 4 cut(s) 75, 131, 315, 639
SspDI GGCGCC 1 cut(s) 905
SspI AATATT 1 cut(s) 591
StuI AGGCCT 1 cut(s) 540
StyD4I CCNGG 1 cut(s) 687
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 1 cut(s) 121
TaqI TCGA 2 cut(s) 41, 197
TaqII GACCGA 5 cut(s) 72, 152, 453, 919, 1079
TasI AATT 6 cut(s) 334, 453, 474, 581, 679, 798
TatI WGTACW 2 cut(s) 777, 1020
TfiI GAWTC 2 cut(s) 158, 256
Tru1I TTAA 6 cut(s) 222, 456, 650, 819, 1053, 1123
Tru9I TTAA 6 cut(s) 222, 456, 650, 819, 1053, 1123
TscAI CASTG 3 cut(s) 171, 300, 409
TseFI GTSAC 1 cut(s) 855
TseI GCWGC 5 cut(s) 128, 320, 323, 526, 1072
Tsp45I GTSAC 1 cut(s) 855
TspDTI ATGAA 2 cut(s) 329, 474
TspGWI ACGGA 1 cut(s) 681
TspRI CASTG 3 cut(s) 171, 300, 409
Tth111I GACNNNGTC 2 cut(s) 70, 1037
VpaK11BI GGWCC 1 cut(s) 741
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 3 cut(s) 334, 581, 679
XceI RCATGY 1 cut(s) 382
ZrmI AGTACT 1 cut(s) 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.