Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0004893

Physical Location & Seq

Genomic Coordinates Forward (+)

19196152 .. 19199599

Gene Structure 3448 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g16490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

ATGGAAAATAAGTATGGTGAACTGGTGGGAAACCAAATAGCCACATGCCGCTCTCTTCTCTCTCTTGGGGTTGAATTGTGTAGGTTACGTATGCGTGAACATCTCCAAATTTCAGAGCTGCGAGCCAAATTCCACGGTCAAAATCCCAACTTCGAATTGGGCATTGGAGACTCAGACGACAATCTCAGTTCCGAAAGCATGGAGGTGAAGAGAGTCATAGCAATTTGTCAGTCGGGGGGTGAATTCTTTACAGAGAAAGATGGTACATTGTCATATAGGGGCAAAGATGCTCATGCTATTGACATTGATGACCAGATGAAGTTTGATGAGTTTAAATTGGAGGTCACAGAAATGTTTGGTTGCGGCTACAATAACATGGTTATCAAGTACTTCCTCCCTGGCAACAAGAAGACCCTGATTACTGTTTCCAATGACAAGGATCTCCAGCGCATGATCAAATTTCATGGTGATTTTGCAACAATTGATATTTATGTCATGGAGGAAGTAGCTCCTCCTGATGTCTCGAACATGCCTCCCAGTAGGTCAAGCAGAACAACTCTGTCAGAAACTGTGGTTCCTGTTGATGCATCTCTTGATGTCGTGGACTTTGTGGGTGATACCACTCAGCCTGATATCCCACTTGATGCTTCTCTTGATGTTGTGGACGGTGCCAGCCCTATTGATGCACATATTGATATACCCACTGAAATATCACCCATTTTTCCTCTTGTTGGCCCTAGCGGTGAGAAGCATGCTAAAGGTGCACACCAGTGGCAGAATGCTATTACGGGTGTGGGCCAAAGATTCAGCAGCGTTCATGAATTTCGTGAATCATTGCGCAAATATGCCATTGCGCATCAGTTCGCCTTTAGGTATAAGAAGAATGATAGTCATCGTGTGACTGTGAAGTGCAAGGCTGAAGGCTGCCCTTGGAGAATTCATGCATCAAGGTTGTCGACAACTCAGTTAATATGTATCAAGAAAATGAATCCAACACATTCCTGTGAAGGTGCTGTTGCAACTACAGGGCATCAGGCGACGAGGAGTTGGGTGGCCAGTATTATCAAGGAGAAGTTAAAATATATGCCCAACTATAAGCCCAAGGATATTGTGAACGATATCAAGCAGGAATATGGTATACAGCTAAACTACTTCCAGGCCTGGCGTGGGAAAGAAATAGCAAAGGAGCAGCTTCAGGGTTCGTACAAGGAGGCATATAATCAATTACCATTTTTCTGTGACAGGATAATGGAGACAAACCCTGGTAGTCTTGCTACTTTTACCACCAAGGAAGACTCCAGTTTCCATCGCCTCTTTGTCGCATTCCATGCCTCATTGTATGGTTTCCAGCAAGGTTGCAGGCCTCTCCTTTTCCTGGATAGCATTCCCTTAAAATCGAAATATCAAGGCACCTTGTTGGCTGCAACAGCTGCTGACGGGGATGATGGGGTATTCCCTGTTGCTTTCACTGTGGTGGATGCAGAAACTGATGATAATTGGCATTGGTTTTTACTACAGTTGAAATCTGCATTGTCAATATCTTGTCCTGTGACATTTGTGGCAGACAGACAGAAGGGATTGAAGGAATCAATTGCGGAAATATTTAAAGACTCATATCATGGCTATTGCCTGCGATATTTGACCGAGCAACTTATCAGGGACTTGAAAGGGCAGTTTTCTCATGAGGTCAAGCGGCTAATGATTGAGGATTTTTACGGTGCTGCTTATGCGCATAGGCCTGAAAACTTTCAAAGTTGTCTTGAAAGCATAAAAAGCATCTCACTGGAGGCTTACAATTGGATCATACAAAGTGAGCCCCAGAAGTGGGCGAATTCATTCTTTCAAGGTGCTCGATATAACCATATGACATCCAACTTTGGTGAGCTGTTCTATAGTTGGGCATCGGATGCACACGAGTTGCCAATAACACAGATGGTTGATGTGATCAGGGGTAAGATTATGGAGTTGATCTACACAAGAAGGGAAGGATCTAATGAATGGTTGACGAGGCTTACTCCAACCATGGAGGAAAAGCTAGAAAAGGAAACTCAGAACATCGGAAACCTCCAAGTGCTATTGTCGACTGGTAGCAGATTTGAGGTTCATGGTGACACCACTGAAGTTGTAGATGTTGATCGCTGGGACTGTAGTTGTAGAGGGTGGCAGATAACTGGTTTACCATGCTGTCATGCAATTGCTGTCATTCGTTGCATGGGCAGGAGCCCATATGATTATTGTTCAAGATACTTTACCACTGAGAGCTATAGATTGACATACTCAGAACCAATATATCCTGTTCCAAATGTAGACATGCCTGTGACGAAGGCTTCTTCTCAAGTACTAGTGACCGTAACCCCTCCGCCCACCCGGCGTCCACCAGGCAGGCCTACTACTAAGAAATATGGATCACAAGATATGGCTAAGCGTCAACTCCAGTGCAGCAGATGCAAGGGTCTTGGGCACAACAAGTCCACTTGCAAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DBD_Tnp_Mut MULE PB1 SWIM

Protein Analysis

829

Amino Acids

93.8

Weight (kDa)

6.41

Isoelectric Point (pI)

37.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PB1	PF00564	90 - 157	1e-07	PB1 domain
DBD_Tnp_Mut	PF03108	261 - 326	6.2e-31	MuDR family transposase
MULE	PF10551	457 - 548	5.9e-13	MULE transposase domain
SWIM	PF04434	708 - 737	1.5e-09	SWIM zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004893)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05850
fragaria_vesca	FvH4_6g08441 FvH4_6g08441 FvH4_6g08441 FvH4_6g08441 FvH4_6g08441 FvH4_6g08441 FvH4_6g08441 FvH4_6g08441
malus_domestica	MD04G1186800.v1.1 MD12G1200300.v1.1
prunus_persica	Prupe.6G305400_v2.0.a1 Prupe.6G305400_v2.0.a1 Prupe.6G305400_v2.0.a1 Prupe.6G305400_v2.0.a1
pyrus_communis	pycom04g16490
rosa_chinensis	RchiOBHm_Chr3g0458421
rosa_laevigata	RLG00000025158
rosa_roxburghii	Rroxscaffold_6G00421080
rosa_rugosa	Rorug03G0027800 Rorug03G0027900 Rorug03G0028000 Rorug03G0028100 Rorug03G0028200 Rorug03G0028300
rosa_samantha	Rh3AG087600 Rh3BG090400 Rh3CG090700 Rh3DG091300
rosa_wichuraiana	Rw3G007440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	3 cut(s)	839, 855, 1731
AccB1I	GGYRCC	2 cut(s)	668, 1409
AccBSI	CCGCTC	1 cut(s)	51
AccI	GTMKAC	4 cut(s)	956, 1138, 2081, 2307
AciI	CCGC	6 cut(s)	49, 363, 741, 1595, 1693, 2360
AclWI	GGATC	4 cut(s)	447, 1808, 1996, 2413
AcoI	YGGCCR	1 cut(s)	1053
AcsI	RAATTY	7 cut(s)	108, 128, 242, 458, 821, 936, 1831
AcuI	CTGAAG	3 cut(s)	939, 1178, 2139
AcyI	GRCGYC	1 cut(s)	2371
AfaI	GTAC	4 cut(s)	265, 389, 1205, 2340
AfiI	CCNNNNNNNGG	5 cut(s)	731, 1167, 1375, 1824, 1825
AgsI	TTSAA	8 cut(s)	74, 1522, 1582, 1666, 1751, 1763, 1844, 2241
AhlI	ACTAGT	1 cut(s)	2341
AjnI	CCWGG	6 cut(s)	397, 1155, 1160, 1261, 1374, 2377
AjuI	GAANNNNNNNTTGG	2 cut(s)	147, 179
AleI	CACNNNNGTG	2 cut(s)	769, 1472
AluBI	AGCT	8 cut(s)	118, 509, 1144, 1192, 1430, 1885, 2035, 2262
AluI	AGCT	8 cut(s)	118, 509, 1144, 1192, 1430, 1885, 2035, 2262
Alw21I	GWGCWC	2 cut(s)	766, 1852
Alw26I	GTCTC	3 cut(s)	162, 526, 1247
Alw44I	GTGCAC	1 cut(s)	762
AlwI	GGATC	4 cut(s)	447, 1808, 1996, 2413
AlwNI	CAGNNNCTG	3 cut(s)	569, 1433, 1487
AoxI	GGCC	7 cut(s)	733, 796, 1053, 1158, 1361, 1736, 2384
ApaLI	GTGCAC	1 cut(s)	762
ApeKI	GCWGC	8 cut(s)	118, 810, 924, 1189, 1421, 1430, 1721, 2439
ApoI	RAATTY	7 cut(s)	108, 128, 242, 458, 821, 936, 1831
Asp700I	GAANNNNTTC	2 cut(s)	1746, 1835
AspLEI	GCGC	4 cut(s)	450, 840, 856, 1732
AspS9I	GGNCC	2 cut(s)	734, 796
AsuC2I	CCSGG	1 cut(s)	2368
AsuHPI	GGTGA	9 cut(s)	29, 217, 251, 479, 626, 705, 755, 1892, 2120
AsuII	TTCGAA	1 cut(s)	153
BaeGI	GKGCMC	2 cut(s)	766, 2463
BalI	TGGCCA	1 cut(s)	1055
BanI	GGYRCC	2 cut(s)	668, 1409
BanII	GRGCYC	2 cut(s)	1818, 2225
BauI	CACGAG	1 cut(s)	1913
BbsI	GAAGAC	2 cut(s)	416, 1299
Bbv12I	GWGCWC	2 cut(s)	766, 1852
BbvI	GCAGC	8 cut(s)	105, 822, 911, 1201, 1408, 1417, 1708, 2451
BccI	CCATC	4 cut(s)	254, 1314, 1439, 1927
BciT130I	CCWGG	6 cut(s)	399, 1157, 1162, 1263, 1376, 2379
BclI	TGATCA	2 cut(s)	453, 1944
BcnI	CCSGG	1 cut(s)	2368
BcoDI	GTCTC	3 cut(s)	162, 526, 1247
BcuI	ACTAGT	1 cut(s)	2341
BfaI	CTAG	3 cut(s)	738, 2036, 2342
BfmI	CTRYAG	5 cut(s)	1023, 1514, 1891, 2146, 2263
BglI	GCCNNNNNGGC	1 cut(s)	2368
BlpI	GCTNAGC	1 cut(s)	2421
BmcAI	AGTACT	2 cut(s)	389, 2340
Bme1390I	CCNGG	7 cut(s)	399, 1157, 1162, 1263, 1376, 2368, 2379
BmgT120I	GGNCC	2 cut(s)	734, 796
BmiI	GGNNCC	4 cut(s)	576, 670, 1411, 2222
BmrFI	CCNGG	7 cut(s)	399, 1157, 1162, 1263, 1376, 2368, 2379
BmrI	ACTGGG	1 cut(s)	531
BmuI	ACTGGG	1 cut(s)	531
BpiI	GAAGAC	2 cut(s)	416, 1299
BplI	GAGNNNNNCTC	2 cut(s)	1999, 2031
BpmI	CTGGAG	4 cut(s)	428, 1282, 1805, 2417
Bpu1102I	GCTNAGC	1 cut(s)	2421
Bpu14I	TTCGAA	1 cut(s)	153
BpuEI	CTTGAG	1 cut(s)	2319
BpuMI	CCSGG	1 cut(s)	2368
BsaAI	YACGTR	1 cut(s)	89
BsaBI	GATNNNNATC	2 cut(s)	891, 2133
BsaHI	GRCGYC	1 cut(s)	2371
BsaJI	CCNNGG	7 cut(s)	133, 397, 929, 1101, 1261, 1287, 2022
Bsc4I	CCNNNNNNNGG	5 cut(s)	731, 1167, 1375, 1824, 1825
Bse1I	ACTGG	9 cut(s)	27, 537, 769, 1056, 1299, 1788, 2089, 2176, 2434
Bse3DI	GCAATG	2 cut(s)	833, 849
Bse8I	GATNNNNATC	2 cut(s)	891, 2133
BseBI	CCWGG	6 cut(s)	399, 1157, 1162, 1263, 1376, 2379
BseDI	CCNNGG	7 cut(s)	133, 397, 929, 1101, 1261, 1287, 2022
BseGI	GGATG	4 cut(s)	1447, 1483, 1868, 1912
BseJI	GATNNNNATC	2 cut(s)	891, 2133
BseLI	CCNNNNNNNGG	5 cut(s)	731, 1167, 1375, 1824, 1825
BseMI	GCAATG	2 cut(s)	833, 849
BseMII	CTCAG	7 cut(s)	186, 199, 638, 977, 2063, 2247, 2292
BseNI	ACTGG	9 cut(s)	27, 537, 769, 1056, 1299, 1788, 2089, 2176, 2434
BseRI	GAGGAG	2 cut(s)	501, 1057
BseSI	GKGCMC	2 cut(s)	766, 2463
BseXI	GCAGC	8 cut(s)	105, 822, 911, 1201, 1408, 1417, 1708, 2451
BseYI	CCCAGC	1 cut(s)	2139
BsgI	GTGCAG	1 cut(s)	2458
BshFI	GGCC	7 cut(s)	735, 798, 1055, 1160, 1363, 1738, 2386
BshNI	GGYRCC	2 cut(s)	668, 1409
BsiHKAI	GWGCWC	2 cut(s)	766, 1852
BsiSI	CCGG	1 cut(s)	2368
BslFI	GGGAC	2 cut(s)	1673, 2156
BslI	CCNNNNNNNGG	5 cut(s)	731, 1167, 1375, 1824, 1825
BsmAI	GTCTC	3 cut(s)	162, 526, 1247
BsmFI	GGGAC	2 cut(s)	1673, 2156
BsmI	GAATGC	3 cut(s)	784, 1322, 1383
BsnI	GGCC	7 cut(s)	735, 798, 1055, 1160, 1363, 1738, 2386
Bsp119I	TTCGAA	1 cut(s)	153
Bsp1286I	GDGCHC	5 cut(s)	766, 1818, 1852, 2225, 2463
Bsp143I	GATC	8 cut(s)	439, 453, 1800, 1944, 1968, 1988, 2134, 2405
Bsp1720I	GCTNAGC	1 cut(s)	2421
Bsp19I	CCATGG	1 cut(s)	2022
BspACI	CCGC	6 cut(s)	49, 363, 741, 1595, 1693, 2360
BspANI	GGCC	7 cut(s)	735, 798, 1055, 1160, 1363, 1738, 2386
BspCNI	CTCAG	7 cut(s)	185, 198, 637, 976, 2062, 2248, 2291
BspHI	TCATGA	2 cut(s)	817, 1681
BspLI	GGNNCC	4 cut(s)	576, 670, 1411, 2222
BspPI	GGATC	4 cut(s)	447, 1808, 1996, 2413
BspT104I	TTCGAA	1 cut(s)	153
BspT107I	GGYRCC	2 cut(s)	668, 1409
BsrBI	CCGCTC	1 cut(s)	51
BsrDI	GCAATG	2 cut(s)	833, 849
BsrI	ACTGG	9 cut(s)	27, 537, 769, 1056, 1299, 1788, 2089, 2176, 2434
BssECI	CCNNGG	7 cut(s)	133, 397, 929, 1101, 1261, 1287, 2022
BssMI	GATC	8 cut(s)	439, 453, 1800, 1944, 1968, 1988, 2134, 2405
BssNAI	GTATAC	1 cut(s)	1139
BssNI	GRCGYC	1 cut(s)	2371
BssSI	CACGAG	1 cut(s)	1913
BssT1I	CCWWGG	4 cut(s)	929, 1101, 1287, 2022
Bst1107I	GTATAC	1 cut(s)	1139
Bst2BI	CACGAG	1 cut(s)	1913
Bst2UI	CCWGG	6 cut(s)	399, 1157, 1162, 1263, 1376, 2379
Bst6I	CTCTTC	2 cut(s)	60, 203
BstACI	GRCGYC	1 cut(s)	2371
BstAPI	GCANNNNNTGC	4 cut(s)	1328, 1430, 1907, 2445
BstBAI	YACGTR	1 cut(s)	89
BstBI	TTCGAA	1 cut(s)	153
BstC8I	GCNNGC	6 cut(s)	123, 673, 753, 1361, 1631, 2384
BstDEI	CTNAG	9 cut(s)	172, 185, 624, 963, 2049, 2256, 2278, 2394, 2421
BstDSI	CCRYGG	2 cut(s)	133, 2022
BstF5I	GGATG	4 cut(s)	1447, 1483, 1868, 1912
BstHHI	GCGC	4 cut(s)	450, 840, 856, 1732
BstKTI	GATC	8 cut(s)	442, 456, 1803, 1947, 1971, 1991, 2137, 2408
BstMAI	GTCTC	3 cut(s)	162, 526, 1247
BstMBI	GATC	8 cut(s)	439, 453, 1800, 1944, 1968, 1988, 2134, 2405
BstMWI	GCNNNNNNNGC	9 cut(s)	761, 1328, 1427, 1430, 1727, 1773, 1907, 2368, 2445
BstNI	CCWGG	6 cut(s)	399, 1157, 1162, 1263, 1376, 2379
BstNSI	RCATGY	4 cut(s)	48, 532, 755, 2314
BstSCI	CCNGG	7 cut(s)	397, 1155, 1160, 1261, 1374, 2366, 2377
BstSFI	CTRYAG	5 cut(s)	1023, 1514, 1891, 2146, 2263
BstSLI	GKGCMC	2 cut(s)	766, 2463
BstSNI	TACGTA	1 cut(s)	89
BstV1I	GCAGC	8 cut(s)	105, 822, 911, 1201, 1408, 1417, 1708, 2451
BstV2I	GAAGAC	2 cut(s)	416, 1299
BstX2I	RGATCY	2 cut(s)	439, 1988
BstYI	RGATCY	2 cut(s)	439, 1988
BstZ17I	GTATAC	1 cut(s)	1139
BsuRI	GGCC	7 cut(s)	735, 798, 1055, 1160, 1363, 1738, 2386
BtgI	CCRYGG	2 cut(s)	133, 2022
BtgZI	GCGATG	1 cut(s)	1292
BtsCI	GGATG	4 cut(s)	1447, 1483, 1868, 1912
BtsIMutI	CAGTG	7 cut(s)	702, 776, 1467, 1781, 2115, 2253, 2441
Cac8I	GCNNGC	6 cut(s)	123, 673, 753, 1361, 1631, 2384
CaiI	CAGNNNCTG	3 cut(s)	569, 1433, 1487
CciI	TCATGA	2 cut(s)	817, 1681
CfoI	GCGC	4 cut(s)	450, 840, 856, 1732
Cfr13I	GGNCC	2 cut(s)	734, 796
CseI	GACGC	2 cut(s)	2360, 2414
Csp6I	GTAC	4 cut(s)	264, 388, 1204, 2339
CspCI	CAANNNNNGTGG	2 cut(s)	2104, 2139
CviQI	GTAC	4 cut(s)	264, 388, 1204, 2339
DdeI	CTNAG	9 cut(s)	172, 185, 624, 963, 2049, 2256, 2278, 2394, 2421
DpnI	GATC	8 cut(s)	441, 455, 1802, 1946, 1970, 1990, 2136, 2407
DpnII	GATC	8 cut(s)	439, 453, 1800, 1944, 1968, 1988, 2134, 2405
DraI	TTTAAA	2 cut(s)	334, 1606
EaeI	YGGCCR	1 cut(s)	1053
Eam1104I	CTCTTC	2 cut(s)	60, 203
EarI	CTCTTC	2 cut(s)	60, 203
EciI	GGCGGA	1 cut(s)	2349
Eco105I	TACGTA	1 cut(s)	89
Eco130I	CCWWGG	4 cut(s)	929, 1101, 1287, 2022
Eco147I	AGGCCT	4 cut(s)	1160, 1363, 1738, 2386
Eco24I	GRGCYC	2 cut(s)	1818, 2225
Eco32I	GATATC	2 cut(s)	634, 1120
Eco57I	CTGAAG	3 cut(s)	939, 1178, 2139
EcoRI	GAATTC	3 cut(s)	242, 936, 1831
EcoRII	CCWGG	6 cut(s)	397, 1155, 1160, 1261, 1374, 2377
EcoRV	GATATC	2 cut(s)	634, 1120
EcoT14I	CCWWGG	4 cut(s)	929, 1101, 1287, 2022
EcoT22I	ATGCAT	2 cut(s)	589, 946
EcoT38I	GRGCYC	2 cut(s)	1818, 2225
ErhI	CCWWGG	4 cut(s)	929, 1101, 1287, 2022
FaqI	GGGAC	2 cut(s)	1673, 2156
FauNDI	CATATG	2 cut(s)	1863, 2227
FbaI	TGATCA	2 cut(s)	453, 1944
FblI	GTMKAC	4 cut(s)	956, 1138, 2081, 2307
FokI	GGATG	4 cut(s)	1454, 1490, 1855, 1919
FriOI	GRGCYC	2 cut(s)	1818, 2225
FspAI	RTGCGCAY	1 cut(s)	1731
FspBI	CTAG	3 cut(s)	738, 2036, 2342
FspI	TGCGCA	3 cut(s)	839, 855, 1731
GlaI	GCGC	4 cut(s)	449, 839, 855, 1731
GsaI	CCCAGC	1 cut(s)	2143
GsuI	CTGGAG	4 cut(s)	428, 1282, 1805, 2417
HaeIII	GGCC	7 cut(s)	735, 798, 1055, 1160, 1363, 1738, 2386
HapII	CCGG	1 cut(s)	2368
HgaI	GACGC	2 cut(s)	2360, 2414
HhaI	GCGC	4 cut(s)	450, 840, 856, 1732
Hin1I	GRCGYC	1 cut(s)	2371
Hin6I	GCGC	4 cut(s)	448, 838, 854, 1730
HinP1I	GCGC	4 cut(s)	448, 838, 854, 1730
HincII	GTYRAC	4 cut(s)	957, 2004, 2082, 2429
HindII	GTYRAC	4 cut(s)	957, 2004, 2082, 2429
HinfI	GANTC	8 cut(s)	170, 213, 804, 830, 988, 1295, 1586, 1610
HpaII	CCGG	1 cut(s)	2368
HphI	GGTGA	9 cut(s)	29, 217, 251, 479, 626, 705, 755, 1892, 2120
Hpy188I	TCNGA	8 cut(s)	115, 175, 193, 565, 1906, 2052, 2060, 2281
Hpy99I	CGWCG	1 cut(s)	1042
HpyAV	CCTTC	7 cut(s)	914, 1001, 1567, 1576, 1974, 1979, 2317
HpyCH4IV	ACGT	1 cut(s)	88
HpyF10VI	GCNNNNNNNGC	9 cut(s)	761, 1328, 1427, 1430, 1727, 1773, 1907, 2368, 2445
HpyF3I	CTNAG	9 cut(s)	172, 185, 624, 963, 2049, 2256, 2278, 2394, 2421
HpySE526I	ACGT	1 cut(s)	88
Hsp92I	GRCGYC	1 cut(s)	2371
HspAI	GCGC	4 cut(s)	448, 838, 854, 1730
Ksp22I	TGATCA	2 cut(s)	453, 1944
Kzo9I	GATC	8 cut(s)	439, 453, 1800, 1944, 1968, 1988, 2134, 2405
LmnI	GCTCC	3 cut(s)	514, 1186, 2220
Lsp1109I	GCAGC	8 cut(s)	105, 822, 911, 1201, 1408, 1417, 1708, 2451
MaeI	CTAG	3 cut(s)	738, 2036, 2342
MaeII	ACGT	1 cut(s)	88
MaeIII	GTNAC	9 cut(s)	84, 343, 898, 1238, 1549, 2108, 2317, 2344, 2350
MalI	GATC	8 cut(s)	441, 455, 1802, 1946, 1970, 1990, 2136, 2407
MbiI	CCGCTC	1 cut(s)	51
MboI	GATC	8 cut(s)	439, 453, 1800, 1944, 1968, 1988, 2134, 2405
MboII	GAAGA	6 cut(s)	47, 220, 421, 892, 1304, 2322
MfeI	CAATTG	4 cut(s)	480, 1590, 1795, 2193
MflI	RGATCY	2 cut(s)	439, 1988
MhlI	GDGCHC	5 cut(s)	766, 1818, 1852, 2225, 2463
MlsI	TGGCCA	1 cut(s)	1055
MluNI	TGGCCA	1 cut(s)	1055
MlyI	GAGTC	4 cut(s)	164, 222, 1289, 1604
MmeI	TCCRAC	3 cut(s)	1016, 1896, 2042
Mox20I	TGGCCA	1 cut(s)	1055
Mph1103I	ATGCAT	2 cut(s)	589, 946
MroXI	GAANNNNTTC	2 cut(s)	1746, 1835
MscI	TGGCCA	1 cut(s)	1055
MseI	TTAA	5 cut(s)	333, 968, 1076, 1391, 1605
MslI	CAYNNNNRTG	6 cut(s)	203, 350, 769, 1002, 1472, 2315
Msp20I	TGGCCA	1 cut(s)	1055
MspA1I	CMGCKG	1 cut(s)	1430
MspI	CCGG	1 cut(s)	2368
MspR9I	CCNGG	7 cut(s)	399, 1157, 1162, 1263, 1376, 2368, 2379
MunI	CAATTG	4 cut(s)	480, 1590, 1795, 2193
Mva1269I	GAATGC	3 cut(s)	784, 1322, 1383
MvaI	CCWGG	6 cut(s)	399, 1157, 1162, 1263, 1376, 2379
MwoI	GCNNNNNNNGC	9 cut(s)	761, 1328, 1427, 1430, 1727, 1773, 1907, 2368, 2445
NciI	CCSGG	1 cut(s)	2368
NcoI	CCATGG	1 cut(s)	2022
NdeI	CATATG	2 cut(s)	1863, 2227
NdeII	GATC	8 cut(s)	439, 453, 1800, 1944, 1968, 1988, 2134, 2405
NlaIV	GGNNCC	4 cut(s)	576, 670, 1411, 2222
NmuCI	GTSAC	7 cut(s)	343, 898, 1238, 1549, 2108, 2317, 2344
NsbI	TGCGCA	3 cut(s)	839, 855, 1731
NsiI	ATGCAT	2 cut(s)	589, 946
NspI	RCATGY	4 cut(s)	48, 532, 755, 2314
NspV	TTCGAA	1 cut(s)	153
OliI	CACNNNNGTG	2 cut(s)	769, 1472
PaeI	GCATGC	1 cut(s)	755
PagI	TCATGA	2 cut(s)	817, 1681
PceI	AGGCCT	4 cut(s)	1160, 1363, 1738, 2386
PctI	GAATGC	3 cut(s)	784, 1322, 1383
PdmI	GAANNNNTTC	2 cut(s)	1746, 1835
PfeI	GAWTC	4 cut(s)	804, 830, 988, 1586
PfoI	TCCNGGA	1 cut(s)	1374
PleI	GAGTC	4 cut(s)	164, 221, 1289, 1604
PpsI	GAGTC	4 cut(s)	164, 221, 1289, 1604
Ppu21I	YACGTR	1 cut(s)	89
Psp6I	CCWGG	6 cut(s)	397, 1155, 1160, 1261, 1374, 2377
PspFI	CCCAGC	1 cut(s)	2139
PspGI	CCWGG	6 cut(s)	397, 1155, 1160, 1261, 1374, 2377
PspN4I	GGNNCC	4 cut(s)	576, 670, 1411, 2222
PspPI	GGNCC	2 cut(s)	734, 796
PstNI	CAGNNNCTG	3 cut(s)	569, 1433, 1487
PsuI	RGATCY	2 cut(s)	439, 1988
PvuII	CAGCTG	1 cut(s)	1430
RsaI	GTAC	4 cut(s)	265, 389, 1205, 2340
RsaNI	GTAC	4 cut(s)	264, 388, 1204, 2339
RseI	CAYNNNNRTG	6 cut(s)	203, 350, 769, 1002, 1472, 2315
SalI	GTCGAC	2 cut(s)	955, 2080
SaqAI	TTAA	5 cut(s)	333, 968, 1076, 1391, 1605
Sau3AI	GATC	8 cut(s)	439, 453, 1800, 1944, 1968, 1988, 2134, 2405
Sau96I	GGNCC	2 cut(s)	734, 796
ScaI	AGTACT	2 cut(s)	389, 2340
SchI	GAGTC	4 cut(s)	164, 222, 1289, 1604
ScrFI	CCNGG	7 cut(s)	399, 1157, 1162, 1263, 1376, 2368, 2379
SduI	GDGCHC	5 cut(s)	766, 1818, 1852, 2225, 2463
SfcI	CTRYAG	5 cut(s)	1023, 1514, 1891, 2146, 2263
SfuI	TTCGAA	1 cut(s)	153
SmiMI	CAYNNNNRTG	6 cut(s)	203, 350, 769, 1002, 1472, 2315
SmlI	CTYRAG	1 cut(s)	2334
SmoI	CTYRAG	1 cut(s)	2334
SnaBI	TACGTA	1 cut(s)	89
SpeI	ACTAGT	1 cut(s)	2341
SphI	GCATGC	1 cut(s)	755
SseBI	AGGCCT	4 cut(s)	1160, 1363, 1738, 2386
SsiI	CCGC	6 cut(s)	49, 363, 741, 1595, 1693, 2360
SspI	AATATT	1 cut(s)	1602
SspMI	CTAG	3 cut(s)	738, 2036, 2342
StuI	AGGCCT	4 cut(s)	1160, 1363, 1738, 2386
StyD4I	CCNGG	7 cut(s)	397, 1155, 1160, 1261, 1374, 2366, 2377
StyI	CCWWGG	4 cut(s)	929, 1101, 1287, 2022
TaiI	ACGT	1 cut(s)	91
TaqI	TCGA	6 cut(s)	153, 524, 956, 1397, 1852, 2081
TaqII	GACCGA	1 cut(s)	1658
TatI	WGTACW	2 cut(s)	387, 2338
TauI	GCSGC	3 cut(s)	51, 366, 1696
TfiI	GAWTC	4 cut(s)	804, 830, 988, 1586
Tru1I	TTAA	5 cut(s)	333, 968, 1076, 1391, 1605
Tru9I	TTAA	5 cut(s)	333, 968, 1076, 1391, 1605
TscAI	CASTG	7 cut(s)	709, 776, 1474, 1788, 2122, 2260, 2441
TseFI	GTSAC	7 cut(s)	343, 898, 1238, 1549, 2108, 2317, 2344
TseI	GCWGC	8 cut(s)	118, 810, 924, 1189, 1421, 1430, 1721, 2439
Tsp45I	GTSAC	7 cut(s)	343, 898, 1238, 1549, 2108, 2317, 2344
TspDTI	ATGAA	9 cut(s)	332, 452, 806, 834, 929, 1001, 1824, 2010, 2093
TspRI	CASTG	7 cut(s)	709, 776, 1474, 1788, 2122, 2260, 2441
VneI	GTGCAC	1 cut(s)	762
XapI	RAATTY	7 cut(s)	108, 128, 242, 458, 821, 936, 1831
XceI	RCATGY	4 cut(s)	48, 532, 755, 2314
XcmI	CCANNNNNNNNNTGG	2 cut(s)	154, 1163
XmiI	GTMKAC	4 cut(s)	956, 1138, 2081, 2307
XmnI	GAANNNNTTC	2 cut(s)	1746, 1835
XspI	CTAG	3 cut(s)	738, 2036, 2342
ZrmI	AGTACT	2 cut(s)	389, 2340
Zsp2I	ATGCAT	2 cut(s)	589, 946

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.