Rorug03G0028200

MuDR family transposase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004893
Physical Location & Seq
Reverse (-)
2242719 .. 2243156
438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0028200.1

Sequence Viewer

Length: 438 bp
ATGTCCGGGAAACAATGCGTCGTGTTTTTGGTGATGGTGTGTGCCGCGATGGCCGGAATTGCTTCTGCGCAAAGTGCTACTAACGTGAGAGCCACGTACCACCTCTACAATCCGCAAGACAACAACTGGGACTTGCGAGCCGTGAGTGCCTATTGCGCGACGTGGGATGCCGACAAGCCCTTGGAATGGCGCAGCAAGTACGGATGGACCGCCTTCTGTCACCCCGCAGGTCCGACCGGACAAGAGGCCTGTGGAAAGTGCCTGCTGGTGACGAACACAGCCACCGGAGCTCAGACGACGGTGAGGATTGTCGACCAGTGCAGCAACGGAGGGCTGGACTTGGACGTTAATGTGTTTAACCAGATAGACACGAACGGAATTGGGTATCAGCAAGGATATCTTACAGTCAACTACGACTTTGTTGACTGTGGGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE PB1 SWIM

Protein Analysis

145

Amino Acids

15.71

Weight (kDa)

4.71

Isoelectric Point (pI)

15.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Barwin PF00967 25 - 143 4.6e-64 Barwin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 69
Acc36I ACCTGC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 312
AccII CGCG 2 cut(s) 47, 158
AciI CCGC 4 cut(s) 45, 113, 210, 225
AcoI YGGCCR 1 cut(s) 51
AfaI GTAC 2 cut(s) 98, 200
AfiI CCNNNNNNNGG 1 cut(s) 186
AjiI CACGTC 1 cut(s) 162
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
Alw21I GWGCWC 1 cut(s) 292
AoxI GGCC 2 cut(s) 51, 246
ApeKI GCWGC 2 cut(s) 192, 321
Asp700I GAANNNNTTC 1 cut(s) 61
AspLEI GCGC 3 cut(s) 70, 158, 192
AspS9I GGNCC 2 cut(s) 207, 230
AsuC2I CCSGG 1 cut(s) 7
AsuHPI GGTGA 4 cut(s) 43, 212, 280, 313
AvaII GGWCC 2 cut(s) 207, 230
BanII GRGCYC 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 2 cut(s) 204, 333
BccI CCATC 3 cut(s) 28, 43, 198
BceAI ACGGC 1 cut(s) 125
BcnI CCSGG 1 cut(s) 7
BfuAI ACCTGC 1 cut(s) 218
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 3 cut(s) 45, 193, 322
BlsI GCNGC 3 cut(s) 46, 194, 323
Bme1390I CCNGG 1 cut(s) 7
Bme18I GGWCC 2 cut(s) 207, 230
BmgBI CACGTC 1 cut(s) 162
BmgT120I GGNCC 2 cut(s) 207, 230
BmrFI CCNGG 1 cut(s) 7
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 157
BmuI ACTGGG 1 cut(s) 136
BpuMI CCSGG 1 cut(s) 7
BsaAI YACGTR 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 180
BsaWI WCCGGW 2 cut(s) 236, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 2 cut(s) 131, 316
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 2 cut(s) 172, 209
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 2 cut(s) 131, 316
BseXI GCAGC 2 cut(s) 204, 333
BsgI GTGCAG 1 cut(s) 340
Bsh1236I CGCG 2 cut(s) 47, 158
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 2 cut(s) 53, 248
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 292
BsiSI CCGG 4 cut(s) 6, 54, 237, 285
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 2 cut(s) 53, 248
Bsp1286I GDGCHC 1 cut(s) 292
BspACI CCGC 4 cut(s) 45, 113, 210, 225
BspANI GGCC 2 cut(s) 53, 248
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 2 cut(s) 47, 158
BspMI ACCTGC 1 cut(s) 218
BsrI ACTGG 2 cut(s) 131, 316
BssECI CCNNGG 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 180
Bst4CI ACNGT 3 cut(s) 301, 406, 428
BstBAI YACGTR 1 cut(s) 96
BstC8I GCNNGC 2 cut(s) 138, 263
BstDEI CTNAG 1 cut(s) 291
BstF5I GGATG 2 cut(s) 172, 209
BstFNI CGCG 2 cut(s) 47, 158
BstHHI GCGC 3 cut(s) 70, 158, 192
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 6 cut(s) 50, 59, 74, 146, 155, 287
BstSCI CCNGG 1 cut(s) 5
BstUI CGCG 2 cut(s) 47, 158
BstV1I GCAGC 2 cut(s) 204, 333
BsuRI GGCC 2 cut(s) 53, 248
BtgZI GCGATG 1 cut(s) 62
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 2 cut(s) 172, 209
BtsIMutI CAGTG 1 cut(s) 323
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 2 cut(s) 138, 263
CfoI GCGC 3 cut(s) 70, 158, 192
Cfr13I GGNCC 2 cut(s) 207, 230
CseI GACGC 1 cut(s) 7
Csp6I GTAC 2 cut(s) 97, 199
CviJI RGCY 8 cut(s) 53, 92, 140, 178, 248, 281, 290, 334
CviKI_1 RGCY 8 cut(s) 53, 92, 140, 178, 248, 281, 290, 334
CviQI GTAC 2 cut(s) 97, 199
DdeI CTNAG 1 cut(s) 291
EaeI YGGCCR 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 180
Eco147I AGGCCT 1 cut(s) 248
Eco24I GRGCYC 1 cut(s) 292
Eco32I GATATC 1 cut(s) 398
Eco47I GGWCC 2 cut(s) 207, 230
Eco53kI GAGCTC 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 290
EcoRV GATATC 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 180
EcoT38I GRGCYC 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 180
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 143
FauI CCCGC 1 cut(s) 232
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 3 cut(s) 45, 193, 322
FokI GGATG 2 cut(s) 179, 216
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 3 cut(s) 45, 193, 322
FspI TGCGCA 1 cut(s) 69
GlaI GCGC 3 cut(s) 69, 157, 191
GluI GCNGC 3 cut(s) 45, 193, 322
HaeIII GGCC 2 cut(s) 53, 248
HapII CCGG 4 cut(s) 6, 54, 237, 285
HgaI GACGC 1 cut(s) 7
HhaI GCGC 3 cut(s) 70, 158, 192
Hin6I GCGC 3 cut(s) 68, 156, 190
HinP1I GCGC 3 cut(s) 68, 156, 190
HincII GTYRAC 3 cut(s) 313, 409, 424
HindII GTYRAC 3 cut(s) 313, 409, 424
HpaII CCGG 4 cut(s) 6, 54, 237, 285
HphI GGTGA 4 cut(s) 43, 212, 280, 313
Hpy166II GTNNAC 3 cut(s) 313, 409, 424
Hpy188I TCNGA 2 cut(s) 234, 294
Hpy8I GTNNAC 3 cut(s) 313, 409, 424
Hpy99I CGWCG 3 cut(s) 23, 163, 301
HpyAV CCTTC 1 cut(s) 223
HpyCH4III ACNGT 3 cut(s) 301, 406, 428
HpyCH4IV ACGT 4 cut(s) 84, 95, 161, 345
HpyCH4V TGCA 1 cut(s) 321
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 59, 74, 146, 155, 287
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 4 cut(s) 84, 95, 161, 345
HspAI GCGC 3 cut(s) 68, 156, 190
LmnI GCTCC 1 cut(s) 287
Lsp1109I GCAGC 2 cut(s) 204, 333
LweI GCATC 1 cut(s) 157
MaeII ACGT 4 cut(s) 84, 95, 161, 345
MaeIII GTNAC 2 cut(s) 218, 268
MhlI GDGCHC 1 cut(s) 292
MluCI AATT 2 cut(s) 57, 378
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 4 cut(s) 113, 238, 297, 323
MroXI GAANNNNTTC 1 cut(s) 61
MseI TTAA 2 cut(s) 348, 357
MspI CCGG 4 cut(s) 6, 54, 237, 285
MspR9I CCNGG 1 cut(s) 7
MvnI CGCG 2 cut(s) 47, 158
MwoI GCNNNNNNNGC 6 cut(s) 50, 59, 74, 146, 155, 287
NciI CCSGG 1 cut(s) 7
NmuCI GTSAC 2 cut(s) 218, 268
NsbI TGCGCA 1 cut(s) 69
PceI AGGCCT 1 cut(s) 248
PdmI GAANNNNTTC 1 cut(s) 61
PfoI TCCNGGA 1 cut(s) 5
PkrI GCNGC 3 cut(s) 46, 194, 323
Ppu21I YACGTR 1 cut(s) 96
Psp124BI GAGCTC 1 cut(s) 292
PspPI GGNCC 2 cut(s) 207, 230
RsaI GTAC 2 cut(s) 98, 200
RsaNI GTAC 2 cut(s) 97, 199
SacI GAGCTC 1 cut(s) 292
SalI GTCGAC 1 cut(s) 311
SaqAI TTAA 2 cut(s) 348, 357
SatI GCNGC 3 cut(s) 45, 193, 322
Sau96I GGNCC 2 cut(s) 207, 230
ScrFI CCNGG 1 cut(s) 7
SduI GDGCHC 1 cut(s) 292
SetI ASST 7 cut(s) 87, 98, 105, 164, 232, 292, 348
SfaNI GCATC 1 cut(s) 157
SinI GGWCC 2 cut(s) 207, 230
Sse9I AATT 2 cut(s) 57, 378
SseBI AGGCCT 1 cut(s) 248
SsiI CCGC 4 cut(s) 45, 113, 210, 225
SstI GAGCTC 1 cut(s) 292
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 1 cut(s) 5
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 3 cut(s) 301, 406, 428
TaiI ACGT 4 cut(s) 87, 98, 164, 348
TaqI TCGA 1 cut(s) 312
TasI AATT 2 cut(s) 57, 378
TauI GCSGC 1 cut(s) 47
Tru1I TTAA 2 cut(s) 348, 357
Tru9I TTAA 2 cut(s) 348, 357
TscAI CASTG 1 cut(s) 323
TseFI GTSAC 2 cut(s) 218, 268
TseI GCWGC 2 cut(s) 192, 321
Tsp45I GTSAC 2 cut(s) 218, 268
TspGWI ACGGA 3 cut(s) 216, 342, 390
TspRI CASTG 1 cut(s) 323
VpaK11BI GGWCC 2 cut(s) 207, 230
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.