Rorug03G0027800

MuDR family transposase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004893
Physical Location & Seq
Reverse (-)
2152812 .. 2153428
617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0027800.1

Sequence Viewer

Length: 438 bp
ATGGCCGGGAAACAATGCATCGTGTTTTTGGTGATGGTGTCTGCCGCGATGGCCGGAATTGCTTCTGCGCAAAGTGCTACTAACGTGAGAGCCACGTACCACCTCTACAATCCGCAGGACAACAACTGGGACTTGCGAGCCGTGAGTGCCTACTGCGCGACGTGGGATGCCGACAAGCCCTTGGAATGGCGCAGCAAGTACGGATGGACCGCCTTCTGTGGCCCCCAGGGCCCGACCGGACAAGCGGCCTGTGGAAAGTGCCTCCGAGTGACGAACACCGCGACAGGAGCTCAGGCGACGGTGAGGATTGTCGACCAGTGCAGCAACGGAGGGCTGGACTTGGACGTGAATGTGTTTAACCAGATAGACACGGACAAAAGTGGGTATCAGCAAGGACATCTTACAGTCAACTACGACTTTGTCGACTGTGGGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE PB1 SWIM

Protein Analysis

145

Amino Acids

15.64

Weight (kDa)

5.14

Isoelectric Point (pI)

14.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Barwin PF00967 25 - 143 1.5e-64 Barwin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 69
AccI GTMKAC 2 cut(s) 312, 423
AccII CGCG 3 cut(s) 47, 158, 281
AciI CCGC 5 cut(s) 45, 113, 210, 245, 279
AcoI YGGCCR 2 cut(s) 3, 51
AfaI GTAC 2 cut(s) 98, 200
AfiI CCNNNNNNNGG 1 cut(s) 186
AjiI CACGTC 2 cut(s) 162, 346
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
Alw21I GWGCWC 1 cut(s) 292
AoxI GGCC 5 cut(s) 3, 51, 220, 229, 246
ApaI GGGCCC 1 cut(s) 233
ApeKI GCWGC 2 cut(s) 192, 321
Asp700I GAANNNNTTC 1 cut(s) 61
AspLEI GCGC 3 cut(s) 70, 158, 192
AspS9I GGNCC 4 cut(s) 207, 221, 229, 230
AsuC2I CCSGG 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 43, 313
AvaII GGWCC 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 233
BanII GRGCYC 2 cut(s) 233, 292
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 2 cut(s) 204, 333
BccI CCATC 3 cut(s) 28, 43, 198
BceAI ACGGC 1 cut(s) 125
BciT130I CCWGG 1 cut(s) 227
BcnI CCSGG 1 cut(s) 7
BglI GCCNNNNNGGC 2 cut(s) 50, 228
BisI GCNGC 4 cut(s) 45, 193, 246, 322
BlsI GCNGC 4 cut(s) 46, 194, 247, 323
Bme1390I CCNGG 2 cut(s) 7, 227
Bme18I GGWCC 1 cut(s) 207
BmgBI CACGTC 2 cut(s) 162, 346
BmgT120I GGNCC 4 cut(s) 207, 221, 229, 230
BmiI GGNNCC 2 cut(s) 223, 231
BmrFI CCNGG 2 cut(s) 7, 227
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 2 cut(s) 27, 157
BmuI ACTGGG 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 7
BsaAI YACGTR 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 180, 225, 226
BsaWI WCCGGW 1 cut(s) 236
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 2 cut(s) 131, 316
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 3 cut(s) 180, 225, 226
BseGI GGATG 2 cut(s) 172, 209
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 2 cut(s) 131, 316
BseSI GKGCMC 1 cut(s) 233
BseXI GCAGC 2 cut(s) 204, 333
BsgI GTGCAG 1 cut(s) 340
Bsh1236I CGCG 3 cut(s) 47, 158, 281
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 5 cut(s) 5, 53, 222, 231, 248
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 292
BsiSI CCGG 3 cut(s) 6, 54, 237
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 5 cut(s) 5, 53, 222, 231, 248
Bsp120I GGGCCC 1 cut(s) 229
Bsp1286I GDGCHC 2 cut(s) 233, 292
BspACI CCGC 5 cut(s) 45, 113, 210, 245, 279
BspANI GGCC 5 cut(s) 5, 53, 222, 231, 248
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 3 cut(s) 47, 158, 281
BspLI GGNNCC 2 cut(s) 223, 231
BsrI ACTGG 2 cut(s) 131, 316
BssECI CCNNGG 3 cut(s) 180, 225, 226
BssT1I CCWWGG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 3 cut(s) 301, 406, 428
BstBAI YACGTR 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 291
BstF5I GGATG 2 cut(s) 172, 209
BstFNI CGCG 3 cut(s) 47, 158, 281
BstHHI GCGC 3 cut(s) 70, 158, 192
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 7 cut(s) 50, 59, 74, 146, 155, 228, 287
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 5, 225
BstSLI GKGCMC 1 cut(s) 233
BstUI CGCG 3 cut(s) 47, 158, 281
BstV1I GCAGC 2 cut(s) 204, 333
BsuRI GGCC 5 cut(s) 5, 53, 222, 231, 248
BtgZI GCGATG 1 cut(s) 62
BtrI CACGTC 2 cut(s) 162, 346
BtsCI GGATG 2 cut(s) 172, 209
BtsIMutI CAGTG 1 cut(s) 323
Cac8I GCNNGC 1 cut(s) 138
CfoI GCGC 3 cut(s) 70, 158, 192
Cfr13I GGNCC 4 cut(s) 207, 221, 229, 230
Csp6I GTAC 2 cut(s) 97, 199
CviQI GTAC 2 cut(s) 97, 199
DdeI CTNAG 1 cut(s) 291
EaeI YGGCCR 2 cut(s) 3, 51
Ecl136II GAGCTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 2 cut(s) 233, 292
Eco47I GGWCC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 290
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 180
EcoT22I ATGCAT 1 cut(s) 20
EcoT38I GRGCYC 2 cut(s) 233, 292
ErhI CCWWGG 1 cut(s) 180
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 143
FblI GTMKAC 2 cut(s) 312, 423
Fnu4HI GCNGC 4 cut(s) 45, 193, 246, 322
FokI GGATG 2 cut(s) 179, 216
FriOI GRGCYC 2 cut(s) 233, 292
Fsp4HI GCNGC 4 cut(s) 45, 193, 246, 322
FspI TGCGCA 1 cut(s) 69
GlaI GCGC 3 cut(s) 69, 157, 191
GluI GCNGC 4 cut(s) 45, 193, 246, 322
HaeIII GGCC 5 cut(s) 5, 53, 222, 231, 248
HapII CCGG 3 cut(s) 6, 54, 237
HhaI GCGC 3 cut(s) 70, 158, 192
Hin6I GCGC 3 cut(s) 68, 156, 190
HinP1I GCGC 3 cut(s) 68, 156, 190
HincII GTYRAC 3 cut(s) 313, 409, 424
HindII GTYRAC 3 cut(s) 313, 409, 424
HpaII CCGG 3 cut(s) 6, 54, 237
HphI GGTGA 2 cut(s) 43, 313
Hpy166II GTNNAC 3 cut(s) 313, 409, 424
Hpy188I TCNGA 1 cut(s) 266
Hpy8I GTNNAC 3 cut(s) 313, 409, 424
Hpy99I CGWCG 2 cut(s) 163, 301
HpyAV CCTTC 1 cut(s) 223
HpyCH4III ACNGT 3 cut(s) 301, 406, 428
HpyCH4IV ACGT 4 cut(s) 84, 95, 161, 345
HpyCH4V TGCA 2 cut(s) 18, 321
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 59, 74, 146, 155, 228, 287
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 4 cut(s) 84, 95, 161, 345
HspAI GCGC 3 cut(s) 68, 156, 190
LmnI GCTCC 1 cut(s) 287
Lsp1109I GCAGC 2 cut(s) 204, 333
LweI GCATC 2 cut(s) 27, 157
MaeII ACGT 4 cut(s) 84, 95, 161, 345
MaeIII GTNAC 1 cut(s) 268
MhlI GDGCHC 2 cut(s) 233, 292
MluCI AATT 1 cut(s) 57
MnlI CCTC 4 cut(s) 113, 272, 297, 323
Mph1103I ATGCAT 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 61
MseI TTAA 1 cut(s) 357
MspI CCGG 3 cut(s) 6, 54, 237
MspR9I CCNGG 2 cut(s) 7, 227
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 3 cut(s) 47, 158, 281
MwoI GCNNNNNNNGC 7 cut(s) 50, 59, 74, 146, 155, 228, 287
NciI CCSGG 1 cut(s) 7
NlaIV GGNNCC 2 cut(s) 223, 231
NmuCI GTSAC 1 cut(s) 268
NsbI TGCGCA 1 cut(s) 69
NsiI ATGCAT 1 cut(s) 20
PasI CCCWGGG 1 cut(s) 226
PcsI WCGNNNNNNNCGW 2 cut(s) 278, 420
PdmI GAANNNNTTC 1 cut(s) 61
PflFI GACNNNGTC 1 cut(s) 419
PkrI GCNGC 4 cut(s) 46, 194, 247, 323
Ppu21I YACGTR 1 cut(s) 96
Psp124BI GAGCTC 1 cut(s) 292
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 223, 231
PspOMI GGGCCC 1 cut(s) 229
PspPI GGNCC 4 cut(s) 207, 221, 229, 230
PsyI GACNNNGTC 1 cut(s) 419
RsaI GTAC 2 cut(s) 98, 200
RsaNI GTAC 2 cut(s) 97, 199
SacI GAGCTC 1 cut(s) 292
SalI GTCGAC 2 cut(s) 311, 422
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 4 cut(s) 45, 193, 246, 322
Sau96I GGNCC 4 cut(s) 207, 221, 229, 230
ScrFI CCNGG 2 cut(s) 7, 227
SduI GDGCHC 2 cut(s) 233, 292
SetI ASST 6 cut(s) 87, 98, 105, 164, 292, 348
SfaNI GCATC 2 cut(s) 27, 157
SfiI GGCCNNNNNGGCC 1 cut(s) 228
SinI GGWCC 1 cut(s) 207
Sse9I AATT 1 cut(s) 57
SsiI CCGC 5 cut(s) 45, 113, 210, 245, 279
SstI GAGCTC 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 5, 225
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 3 cut(s) 301, 406, 428
TaiI ACGT 4 cut(s) 87, 98, 164, 348
TaqI TCGA 2 cut(s) 312, 423
TasI AATT 1 cut(s) 57
TauI GCSGC 2 cut(s) 47, 248
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 1 cut(s) 323
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 2 cut(s) 192, 321
Tsp45I GTSAC 1 cut(s) 268
TspGWI ACGGA 3 cut(s) 216, 342, 386
TspRI CASTG 1 cut(s) 323
Tth111I GACNNNGTC 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 207
XmiI GTMKAC 2 cut(s) 312, 423
XmnI GAANNNNTTC 1 cut(s) 61
Zsp2I ATGCAT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.