Rorug03G0027900

MuDR family transposase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004893
Physical Location & Seq
Forward (+)
2163930 .. 2246867
82938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0027900.1

Sequence Viewer

Length: 660 bp
ATGAGTATGGTGCTACCTGACTTGGACAGATTGCAGCTTGCACAAGACCCACCTGCTGGCGCGGAGATTAGTATGGTGCTCCCAGGTTTGGACAGATTACGGCTTGTAGAACACTCAAGCGCAGGTGGAGAGGATGTTTTTGCAAGGCTGCAGAAAAAAGTGTCGGATGACCCACCCCCGGGCGCAGATTATACTCTTGCAAGGTATAGGTTGTTTAAAGAGTTGAAGAGGCTGCTACAAGACCCACCTGCAGGGATCAGTGTGGTTCCCCCGGACTTCTACAATTGCGATATAATGTTGTGGAATGTTGTTATAGCTCCACATTCCTTGGATTCTCCTTGGGAGGGAGGCGCATTTAAGTTGACTCTTCAGTTTAGTGAGGATTATCCACTCAAACCACCAATGGTGCGATTTGTTTCTCAAATGTTTCATCCAAACATTTCTGCAGATGGAATTATGTCTTTTAATAATTTACTGGATCGGTGGACTTCTATAAATGATGTGGAAGATATACTCAAGTATATCCAGTCCCTAATATGTCATCCCAACCCGAAGTGTCCTGCAAATTCTGAAGCTTTCTACATGTACTTCAGCGAGAACAGACTGGGGTACAACCGAAGAGTGAGGATGATCGTGGATCAGAGTTGGATGGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE PB1 SWIM

Protein Analysis

219

Amino Acids

24.92

Weight (kDa)

5.09

Isoelectric Point (pI)

58.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 70 - 210 3.7e-33 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 61, 113, 256
Acc36I ACCTGC 3 cut(s) 61, 113, 256
AccB7I CCANNNNNTGG 1 cut(s) 56
AccII CGCG 1 cut(s) 62
AciI CCGC 1 cut(s) 62
AclWI GGATC 3 cut(s) 263, 486, 645
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 3 cut(s) 353, 574, 591
AfaI GTAC 2 cut(s) 587, 611
AfiI CCNNNNNNNGG 6 cut(s) 56, 88, 178, 179, 251, 344
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 1 cut(s) 226
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 3 cut(s) 37, 317, 575
AluI AGCT 3 cut(s) 37, 317, 575
Alw21I GWGCWC 1 cut(s) 81
AlwI GGATC 3 cut(s) 263, 486, 645
Ama87I CYCGRG 1 cut(s) 178
ApeKI GCWGC 3 cut(s) 34, 148, 232
ApoI RAATTY 1 cut(s) 565
AspLEI GCGC 4 cut(s) 62, 122, 185, 353
AsuC2I CCSGG 3 cut(s) 179, 180, 272
AvaI CYCGRG 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 3 cut(s) 46, 135, 219
BccI CCATC 2 cut(s) 443, 643
BceAI ACGGC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 84
BcnI CCSGG 3 cut(s) 179, 180, 272
BfaI CTAG 1 cut(s) 658
BfmI CTRYAG 3 cut(s) 149, 249, 444
BfuAI ACCTGC 3 cut(s) 61, 113, 256
BisI GCNGC 3 cut(s) 35, 149, 233
BlsI GCNGC 3 cut(s) 36, 150, 234
Bme1390I CCNGG 4 cut(s) 84, 179, 180, 272
BmeT110I CYCGRG 1 cut(s) 178
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 4 cut(s) 84, 179, 180, 272
BmrI ACTGGG 1 cut(s) 614
BmuI ACTGGG 1 cut(s) 614
BpuEI CTTGAG 2 cut(s) 100, 500
BpuMI CCSGG 3 cut(s) 179, 180, 272
BsaJI CCNNGG 6 cut(s) 82, 177, 178, 270, 327, 338
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 88, 178, 179, 251, 344
Bse1I ACTGG 3 cut(s) 480, 526, 609
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 6 cut(s) 82, 177, 178, 270, 327, 338
BseGI GGATG 6 cut(s) 139, 172, 430, 541, 633, 654
BseLI CCNNNNNNNGG 6 cut(s) 56, 88, 178, 179, 251, 344
BseNI ACTGG 3 cut(s) 480, 526, 609
BseXI GCAGC 3 cut(s) 46, 135, 219
Bsh1236I CGCG 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 81
BsiHKCI CYCGRG 1 cut(s) 178
BsiSI CCGG 2 cut(s) 179, 272
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 6 cut(s) 56, 88, 178, 179, 251, 344
BsmFI GGGAC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 4 cut(s) 255, 478, 630, 637
BspACI CCGC 1 cut(s) 62
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 3 cut(s) 153, 253, 448
BspMI ACCTGC 3 cut(s) 61, 113, 256
BspPI GGATC 3 cut(s) 263, 486, 645
BsrI ACTGG 3 cut(s) 480, 526, 609
BssECI CCNNGG 6 cut(s) 82, 177, 178, 270, 327, 338
BssMI GATC 4 cut(s) 255, 478, 630, 637
BssT1I CCWWGG 2 cut(s) 327, 338
Bst2UI CCWGG 1 cut(s) 84
Bst6I CTCTTC 3 cut(s) 221, 372, 613
BstC8I GCNNGC 2 cut(s) 39, 58
BstF5I GGATG 6 cut(s) 139, 172, 430, 541, 633, 654
BstFNI CGCG 1 cut(s) 62
BstHHI GCGC 4 cut(s) 62, 122, 185, 353
BstKTI GATC 4 cut(s) 258, 481, 633, 640
BstMBI GATC 4 cut(s) 255, 478, 630, 637
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 4 cut(s) 82, 177, 178, 270
BstSFI CTRYAG 3 cut(s) 149, 249, 444
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 3 cut(s) 46, 135, 219
BtsCI GGATG 6 cut(s) 139, 172, 430, 541, 633, 654
BtsIMutI CAGTG 1 cut(s) 265
BveI ACCTGC 3 cut(s) 61, 113, 256
Cac8I GCNNGC 2 cut(s) 39, 58
CfoI GCGC 4 cut(s) 62, 122, 185, 353
Cfr9I CCCGGG 1 cut(s) 178
Csp6I GTAC 2 cut(s) 586, 610
CspCI CAANNNNNGTGG 2 cut(s) 309, 344
CviAII CATG 1 cut(s) 583
CviJI RGCY 6 cut(s) 37, 103, 148, 232, 317, 575
CviKI_1 RGCY 6 cut(s) 37, 103, 148, 232, 317, 575
CviQI GTAC 2 cut(s) 586, 610
DpnI GATC 4 cut(s) 257, 480, 632, 639
DpnII GATC 4 cut(s) 255, 478, 630, 637
DraI TTTAAA 1 cut(s) 217
Eam1104I CTCTTC 3 cut(s) 221, 372, 613
EarI CTCTTC 3 cut(s) 221, 372, 613
Eco130I CCWWGG 2 cut(s) 327, 338
Eco57I CTGAAG 3 cut(s) 353, 574, 591
Eco88I CYCGRG 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 82
EcoT14I CCWWGG 2 cut(s) 327, 338
ErhI CCWWGG 2 cut(s) 327, 338
FaeI CATG 1 cut(s) 586
FaqI GGGAC 1 cut(s) 514
FatI CATG 1 cut(s) 582
Fnu4HI GCNGC 3 cut(s) 35, 149, 233
FokI GGATG 5 cut(s) 146, 179, 417, 528, 640
Fsp4HI GCNGC 3 cut(s) 35, 149, 233
FspBI CTAG 1 cut(s) 658
GlaI GCGC 4 cut(s) 61, 121, 184, 352
GluI GCNGC 3 cut(s) 35, 149, 233
HapII CCGG 2 cut(s) 179, 272
HhaI GCGC 4 cut(s) 62, 122, 185, 353
Hin1II CATG 1 cut(s) 586
Hin6I GCGC 4 cut(s) 60, 120, 183, 351
HinP1I GCGC 4 cut(s) 60, 120, 183, 351
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HindIII AAGCTT 1 cut(s) 573
HinfI GANTC 2 cut(s) 332, 364
HpaII CCGG 2 cut(s) 179, 272
Hpy166II GTNNAC 2 cut(s) 363, 486
Hpy188I TCNGA 3 cut(s) 166, 571, 642
Hpy8I GTNNAC 2 cut(s) 363, 486
HpyCH4V TGCA 8 cut(s) 34, 41, 143, 151, 200, 251, 446, 563
Hsp92II CATG 1 cut(s) 586
HspAI GCGC 4 cut(s) 60, 120, 183, 351
Kzo9I GATC 4 cut(s) 255, 478, 630, 637
LmnI GCTCC 2 cut(s) 84, 322
Lsp1109I GCAGC 3 cut(s) 46, 135, 219
MaeI CTAG 1 cut(s) 658
MalI GATC 4 cut(s) 257, 480, 632, 639
MboI GATC 4 cut(s) 255, 478, 630, 637
MboII GAAGA 4 cut(s) 238, 359, 518, 630
MfeI CAATTG 1 cut(s) 283
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 4 cut(s) 283, 453, 469, 565
MlyI GAGTC 1 cut(s) 358
MmeI TCCRAC 2 cut(s) 144, 626
MnlI CCTC 6 cut(s) 124, 222, 337, 341, 373, 618
MseI TTAA 3 cut(s) 216, 357, 465
MspI CCGG 2 cut(s) 179, 272
MspR9I CCNGG 4 cut(s) 84, 179, 180, 272
MunI CAATTG 1 cut(s) 283
MvaI CCWGG 1 cut(s) 84
MvnI CGCG 1 cut(s) 62
NciI CCSGG 3 cut(s) 179, 180, 272
NdeII GATC 4 cut(s) 255, 478, 630, 637
NlaIII CATG 1 cut(s) 586
NlaIV GGNNCC 1 cut(s) 267
NspI RCATGY 1 cut(s) 586
PaqCI CACCTGC 3 cut(s) 61, 113, 256
PciI ACATGT 1 cut(s) 582
PfeI GAWTC 1 cut(s) 332
PflMI CCANNNNNTGG 1 cut(s) 56
PkrI GCNGC 3 cut(s) 36, 150, 234
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
PscI ACATGT 1 cut(s) 582
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 1 cut(s) 267
PstI CTGCAG 3 cut(s) 153, 253, 448
RsaI GTAC 2 cut(s) 587, 611
RsaNI GTAC 2 cut(s) 586, 610
SaqAI TTAA 3 cut(s) 216, 357, 465
SatI GCNGC 3 cut(s) 35, 149, 233
Sau3AI GATC 4 cut(s) 255, 478, 630, 637
SbfI CCTGCAGG 1 cut(s) 253
SchI GAGTC 1 cut(s) 358
ScrFI CCNGG 4 cut(s) 84, 179, 180, 272
SdaI CCTGCAGG 1 cut(s) 253
SduI GDGCHC 1 cut(s) 81
SfcI CTRYAG 3 cut(s) 149, 249, 444
SmaI CCCGGG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 115, 515
SmoI CTYRAG 2 cut(s) 115, 515
Sse8387I CCTGCAGG 1 cut(s) 253
Sse9I AATT 4 cut(s) 283, 453, 469, 565
SsiI CCGC 1 cut(s) 62
SspMI CTAG 1 cut(s) 658
StyD4I CCNGG 4 cut(s) 82, 177, 178, 270
StyI CCWWGG 2 cut(s) 327, 338
TasI AATT 4 cut(s) 283, 453, 469, 565
TatI WGTACW 1 cut(s) 585
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 3 cut(s) 216, 357, 465
Tru9I TTAA 3 cut(s) 216, 357, 465
TscAI CASTG 1 cut(s) 265
TseI GCWGC 3 cut(s) 34, 148, 232
TspDTI ATGAA 1 cut(s) 419
TspMI CCCGGG 1 cut(s) 178
TspRI CASTG 1 cut(s) 265
Van91I CCANNNNNTGG 1 cut(s) 56
XapI RAATTY 1 cut(s) 565
XceI RCATGY 1 cut(s) 586
XmaI CCCGGG 1 cut(s) 178
XspI CTAG 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.