pycom04g21350

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005781
Physical Location & Seq
Reverse (-)
22553829 .. 22556422
2594 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
ATGGCTCAGCTCTTGAAAGTACCCGCGATCCCCTTCCCTCGAAGTCTAAGACGCAACAGCAGCGGGACGAGCTCACTTCGATTTCACATAGCAACAACAATGGTTTTTACAAAACACAACGCCCGCAACACTACCAAGCCCTCCTCCTTTCGTAGTACCACTCGAAACCTTCCTATAATCCTCTTCGACATCATGGACACACTTGTTCGTGACCCCTTTTACCACGATGTCCCTGCCTTCTTCGGAATGTCTTTCGATGAATTAATCGAATGCAAGCACCCGACTGCATGGATCGAGTTCGAAAAGGGGATTACTGATGAGGTGGAGCTTGCTGCAAGAACATTCTTTAAGGATGGAAGGCCTCTAGATTTGGAAGGTCTGTTGGAGTTCCTCACTATTTTCCAGCCATGTCTGCGGAAGAGGAAGCCCGACCCAGAATTCTATTTGGAAGTTGTGAGCCATCTAAAAGTTGATCCGGGAAGCTGTATCTTTGTCGATGACAGGATAAGAAATGTAGAGGAGGCGAAGGAAGTTGGCATGATCGGTCTACATTTCAAAAATGCAGATTCGCTTCGTCAAGATCTTTCACTGATGGGGATTGATATTTAA

Protein Analysis

203

Amino Acids

23.16

Weight (kDa)

6.31

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 140 - 179 5.9e-06 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 547
AccII CGCG 1 cut(s) 26
AciI CCGC 4 cut(s) 24, 63, 124, 415
AclWI GGATC 3 cut(s) 22, 299, 467
AcsI RAATTY 1 cut(s) 437
AfaI GTAC 2 cut(s) 21, 157
AgsI TTSAA 2 cut(s) 16, 556
AluBI AGCT 4 cut(s) 10, 72, 328, 483
AluI AGCT 4 cut(s) 10, 72, 328, 483
Alw21I GWGCWC 1 cut(s) 74
AlwI GGATC 3 cut(s) 22, 299, 467
AoxI GGCC 1 cut(s) 359
ApeKI GCWGC 2 cut(s) 60, 332
ApoI RAATTY 1 cut(s) 437
AseI ATTAAT 1 cut(s) 263
AsuC2I CCSGG 1 cut(s) 477
AsuII TTCGAA 1 cut(s) 300
BanII GRGCYC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 2 cut(s) 72, 319
BccI CCATC 3 cut(s) 347, 468, 586
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BcnI CCSGG 1 cut(s) 477
BfaI CTAG 1 cut(s) 365
BglII AGATCT 1 cut(s) 580
BisI GCNGC 2 cut(s) 61, 333
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 2 cut(s) 62, 334
Bme1390I CCNGG 1 cut(s) 477
BmrFI CCNGG 1 cut(s) 477
Bpu1102I GCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 300
BpuMI CCSGG 1 cut(s) 477
BseGI GGATG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 20
BseRI GAGGAG 2 cut(s) 133, 533
BseXI GCAGC 2 cut(s) 72, 319
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 74
BsiSI CCGG 1 cut(s) 476
BslFI GGGAC 2 cut(s) 79, 215
BsmFI GGGAC 2 cut(s) 79, 215
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 361
Bsp119I TTCGAA 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 74
Bsp143I GATC 5 cut(s) 27, 291, 472, 540, 580
Bsp1720I GCTNAGC 1 cut(s) 6
BspACI CCGC 4 cut(s) 24, 63, 124, 415
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 1 cut(s) 26
BspPI GGATC 3 cut(s) 22, 299, 467
BspT104I TTCGAA 1 cut(s) 300
BssMI GATC 5 cut(s) 27, 291, 472, 540, 580
Bst6I CTCTTC 2 cut(s) 188, 413
BstBI TTCGAA 1 cut(s) 300
BstC8I GCNNGC 3 cut(s) 124, 275, 330
BstDEI CTNAG 2 cut(s) 6, 47
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 1 cut(s) 26
BstKTI GATC 5 cut(s) 30, 294, 475, 543, 583
BstMBI GATC 5 cut(s) 27, 291, 472, 540, 580
BstMWI GCNNNNNNNGC 3 cut(s) 60, 69, 412
BstSCI CCNGG 1 cut(s) 475
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 72, 319
BstX2I RGATCY 1 cut(s) 580
BstYI RGATCY 1 cut(s) 580
BsuRI GGCC 1 cut(s) 361
BtsCI GGATG 1 cut(s) 358
BtsIMutI CAGTG 1 cut(s) 587
Cac8I GCNNGC 3 cut(s) 124, 275, 330
CseI GACGC 1 cut(s) 60
Csp6I GTAC 2 cut(s) 20, 156
CviAII CATG 4 cut(s) 193, 288, 408, 538
CviQI GTAC 2 cut(s) 20, 156
DdeI CTNAG 2 cut(s) 6, 47
DpnI GATC 5 cut(s) 29, 293, 474, 542, 582
DpnII GATC 5 cut(s) 27, 291, 472, 540, 580
Eam1104I CTCTTC 2 cut(s) 188, 413
EarI CTCTTC 2 cut(s) 188, 413
Ecl136II GAGCTC 1 cut(s) 72
Eco147I AGGCCT 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 74
Eco53kI GAGCTC 1 cut(s) 72
EcoICRI GAGCTC 1 cut(s) 72
EcoRI GAATTC 1 cut(s) 437
EcoT38I GRGCYC 1 cut(s) 74
FaeI CATG 4 cut(s) 196, 291, 411, 541
FaiI YATR 6 cut(s) 89, 176, 194, 289, 409, 539
FaqI GGGAC 2 cut(s) 79, 215
FatI CATG 4 cut(s) 192, 287, 407, 537
FauI CCCGC 3 cut(s) 31, 56, 131
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 61, 333
FokI GGATG 1 cut(s) 365
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 2 cut(s) 61, 333
FspBI CTAG 1 cut(s) 365
GluI GCNGC 2 cut(s) 61, 333
HaeIII GGCC 1 cut(s) 361
HapII CCGG 1 cut(s) 476
HgaI GACGC 1 cut(s) 60
Hin1II CATG 4 cut(s) 196, 291, 411, 541
HinfI GANTC 1 cut(s) 566
HpaII CCGG 1 cut(s) 476
Hpy166II GTNNAC 1 cut(s) 548
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 4 cut(s) 13, 209, 365, 578
Hpy8I GTNNAC 1 cut(s) 548
HpyAV CCTTC 6 cut(s) 43, 179, 247, 351, 368, 520
HpyCH4V TGCA 4 cut(s) 273, 287, 335, 563
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 69, 412
HpyF3I CTNAG 2 cut(s) 6, 47
Hsp92II CATG 4 cut(s) 196, 291, 411, 541
Kzo9I GATC 5 cut(s) 27, 291, 472, 540, 580
LmnI GCTCC 1 cut(s) 325
LpnPI CCDG 5 cut(s) 246, 416, 447, 487, 489
Lsp1109I GCAGC 2 cut(s) 72, 319
MaeI CTAG 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 209
MalI GATC 5 cut(s) 29, 293, 474, 542, 582
MboI GATC 5 cut(s) 27, 291, 472, 540, 580
MboII GAAGA 3 cut(s) 175, 232, 430
MflI RGATCY 1 cut(s) 580
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 2 cut(s) 260, 437
MmeI TCCRAC 1 cut(s) 363
MseI TTAA 3 cut(s) 263, 348, 607
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 476
MspR9I CCNGG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 275
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 3 cut(s) 60, 69, 412
NciI CCSGG 1 cut(s) 477
NdeII GATC 5 cut(s) 27, 291, 472, 540, 580
NlaIII CATG 4 cut(s) 196, 291, 411, 541
NmuCI GTSAC 1 cut(s) 209
NspV TTCGAA 1 cut(s) 300
PceI AGGCCT 1 cut(s) 361
PctI GAATGC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 566
PfoI TCCNGGA 1 cut(s) 475
PkrI GCNGC 2 cut(s) 62, 334
PshBI ATTAAT 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 74
PsuI RGATCY 1 cut(s) 580
RsaI GTAC 2 cut(s) 21, 157
RsaNI GTAC 2 cut(s) 20, 156
SacI GAGCTC 1 cut(s) 74
SaqAI TTAA 3 cut(s) 263, 348, 607
SatI GCNGC 2 cut(s) 61, 333
Sau3AI GATC 5 cut(s) 27, 291, 472, 540, 580
ScrFI CCNGG 1 cut(s) 477
SduI GDGCHC 1 cut(s) 74
SetI ASST 7 cut(s) 12, 74, 171, 324, 330, 379, 485
SfuI TTCGAA 1 cut(s) 300
Sse9I AATT 2 cut(s) 260, 437
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 4 cut(s) 24, 63, 124, 415
SspMI CTAG 1 cut(s) 365
SstI GAGCTC 1 cut(s) 74
StuI AGGCCT 1 cut(s) 361
StyD4I CCNGG 1 cut(s) 475
TaqI TCGA 9 cut(s) 40, 79, 163, 186, 255, 267, 294, 300, 495
TaqII GACCGA 1 cut(s) 533
TasI AATT 2 cut(s) 260, 437
TfiI GAWTC 1 cut(s) 566
Tru1I TTAA 3 cut(s) 263, 348, 607
Tru9I TTAA 3 cut(s) 263, 348, 607
TscAI CASTG 1 cut(s) 594
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 2 cut(s) 60, 332
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 273
TspRI CASTG 1 cut(s) 594
VspI ATTAAT 1 cut(s) 263
XapI RAATTY 1 cut(s) 437
XbaI TCTAGA 1 cut(s) 364
XmiI GTMKAC 1 cut(s) 547
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.