Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0008285

Physical Location & Seq

Genomic Coordinates Forward (+)

22727452 .. 22734208

Gene Structure 6757 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2634 bp

ATGGCTCTGCAGTCAGCCGCACTCTCTCCTCGCTCCATCGGCGCATCCACGTCGCTACCCTGCTCCGCCAAGTACCCGATCATTTGCTCCTCCTCTGCTCCCAGTTCGAGCTGCTTCCTTTCGCAGAGGAAGAATGCGGCTGCGGTTGGAATTGGATTGAGTAAGAGGAAGATGGGCTCCTGGGGGTGTACGGTAAGGTCGAACTTGGACACTGTCGGTCCCACTGTGAATGTGGGCCAAGTCACGGAGGTCGACAAGGACACCTTCTGGCCCATCGTCAAGGCCGCCGGCGACAAGACCGTCGTCCTCGACATGTATACCCAATGGTGTGGACCTTGCAAAGTGATGGCTCCAAAATTTCAAGAATTGTCCAAGAAGTATCTTGATGTCGTATTTTTGAAGCTTGATTGTAACCAAGAAAATAAGCCATTGGCGAAGGAGCTTGGGATAAGGGTGGTGCCAACTTTCAAGATTTTGAAGGACAACAAGGTTGTAAAAGAGGTGAAGGGGGCCAAATTCGACGATTTAGTTGTTGCTATAGATGCTGTTAGATCGAGAAAGAGAGAGAAATCAGAAATGTGTGGGATATTTGCGTATCTGAATTACAACGTCAACAGAGAGAGGCGTTACATTCTGCAGGTGCTATTCAATGGCCTGAGGCGTCTGGAATACCGGGGCTACGATTCCGCCGGGATTTCAATCGACCGTTCTTATTCCTTCGATCCAAACACCCCCCAATCTTCCCCGCCTCCCCCCCTCGTTTTCCGCCAGGAGGGAAATATCGAGACCCTCGTCAAATCCGTCTACCAAGAGGTTGCTGAAACAGATTTAAGTTTGGAAGAATCTTTCTCTCACCATGCTGGAATAGCACACACCCGGTGGGCCACTCATGGAGAGCCAGCTCCAAGGAACAGCCATCCACAGAGCTCTGGTTCCGGAAATGAATTTTTGGTTGTTCACAATGGAGTCATCACTAATTATGAGGCCTTAAAGGAATCACTAGTTCGGCACGGATTCACCTTTGAATCTGAAACAGACACAGAAGTAATTCCAAAGCTTGCCAAATATGTTTATGATAAAGCTAAAGAAGGAGGAGGTGACCAGACTATCACATTTAGTCAAGTTGTGCTTGAGGTTATGAGGCATCTTGAAGGTGCATATGCCCTGATTTTTAAAAGCCAGCATTATCCAAATGAGTTGATTGCTTGCAAGCGTGGCAGTCCGTTGCTCCTTGGTGTCAAAGAATTTAACGAAAATGCTAGCGGTGGATCAGCATTTCACGATGATAATTTCCTTTCAAAAAGTGGACATGCTAAAGAACTCTTTTTGTCCAGTGATGCCAATGCTGTAGTTGAACACACTAAAAAGGTGTTGGTGATTGAGGATGGTGAAGTGGTACATCTGAAGGATGGAGGTGTTTCTATCCTGAAGTTTGATAAAGGACAGCATGGTGGACTTTCAAGAGTTGCATCAGTACAACGAGCGTTGTCCATTCTTGAGATGGAGGTTGAACAAATAAACAAAGGGAGTTACAAACATTATATGCAGAAAGAAATTCATGAACAGCCTGAATCTTTGACTACCACAATGAGGGGGAGGCTTTTACGTGGAGGTTCCTGCAAAGCCAAGACTGTTCTCCTTGGTGGACTGAAGGATCACCTTAAAACAATTAGGCGAAGCAGGAGAATTGTTTTCATCGGTTGTGGTACAAGCTACAATGCTGCTTTAGCTGCAAGACCCATCTTGGAAGAACTTTCTGGTATTCCTGTCACAATGGAAATTGCTAGTGATTTGTTGGATCGGCAAGGACCTATATACAGAGAAGATACAGCTGTCTTTGTCAGTCAATCTGGTGAAACAGCAGATACTCTGATTGCTTTGGAATATGCGTTAGAAAATGGTGCATTATGTGTTGGCATAACAAATACTGTTGATAGTGCTATTGCCAGGAATACACACTGCGGTGTTCATATAAATGCTGGCGCTGAGATTGGTGTGGCAAGCACCAAGGCATACACGAGTCAAATTGTGGTGATGGCTATGCTAGCACTAGCTATAGGTGGTGACACAATTTCCAATCAAGCACGAAGAGAGGCTATAATTGACGGTTTATTTGAGTTGCCAAACAAAGTCAGAGAGGTCCTGAAGCTTGATCAGATGATGAAGGATCTTGCACAGGAACTGATAGCTCATCAGTCACTTCTTGTGTTTGGGAGAGGATACAACTACGCAACATCTCTAGAGGGCGCTTTGAAGGTAAAGGAAGTGGCCCTTATGCACAGTGAAGGAATACTAGCGGGTGAAATGAAACATGGTCCTTTGGCTTTAGTTGATGAAACTCTCCCTACATTTGTGATTGCCACCCGTGATGCTTGCTTCAGTAAGCAGCAATCAGTTATTCAGCAGCTTCGTGCCCGTAAAGGTCGACTCATAGTTATGTGTTCGAAGGGAGATGCTGCATCAGTGTGCCCCGGGGGGTCTTGTCGAGTGATTGAAGTTCCTCAGCTTGAGGATTGTCTTCAACCCGTGGTTAATATTATTCCATTGCAGCTGTTGGCATACCATCTCACTGTTTTAAGAGGTTTTAACGTGGATCAGCCGCGGAATCTTGCGAAGAGCGTGACTACGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004360 GO:0006002 GO:0006040 GO:0006047 GO:0006139 GO:0006464 GO:0006486 GO:0006487 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008483 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009225 GO:0009987 GO:0016740 GO:0016769 GO:0019538 GO:0019637 GO:0034641 GO:0034645 GO:0036211 GO:0043170 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0046483 GO:0055086 GO:0070085 GO:0070548 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00250 ko00520 ko01100 ko01130 ko04931 map00250 map00520 map01100 map01130 map04931

Pfam Domains

Protein Families

GATase_6 SIS

Protein Analysis

878

Amino Acids

95.96

Weight (kDa)

8.26

Isoelectric Point (pI)

43.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	81 - 180	4.6e-22	Thioredoxin
GATase_6	PF13522	283 - 394	2.2e-14	Glutamine amidotransferase domain
GATase_7	PF13537	305 - 394	5.9e-09	Glutamine amidotransferase domain
SIS	PF01380	557 - 685	1.4e-33	SIS domain
SIS	PF01380	729 - 859	1.1e-17	SIS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24090
fragaria_vesca	FvH4_6g00360 FvH4_6g00360
malus_domestica	MD04G1173900.v1.1 MD04G1246300.v1.1 MD12G1263800.v1.1
prunus_persica	Prupe.6G362600_v2.0.a1
pyrus_communis	pycom04g21700 pycom12g24000
rosa_chinensis	RchiOBHm_Chr3g0447661 RchiOBHm_Chr4g0412421
rosa_laevigata	RLG00000025980
rosa_multiflora	Rmu_ssc0000391.1_g000002
rosa_roxburghii	Rroxscaffold_6G00425870
rosa_rugosa	Rorug02G0604100 Rorug04G0077300 Rorug07G0328400
rosa_samantha	Rh3AG004100 Rh3BG003700 Rh3CG004000 Rh3DG004000 Rh4CG183700
rosa_wichuraiana	Rw3G000310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	628
AasI	GACNNNNNNGTC	1 cut(s)	299
Acc36I	ACCTGC	1 cut(s)	628
AccB1I	GGYRCC	1 cut(s)	457
AccI	GTMKAC	4 cut(s)	252, 317, 804, 2425
AccII	CGCG	1 cut(s)	2602
AccIII	TCCGGA	1 cut(s)	935
AclWI	GGATC	6 cut(s)	716, 1276, 1662, 1806, 2175, 2601
AcsI	RAATTY	5 cut(s)	356, 515, 944, 1244, 1554
AcuI	CTGAAG	5 cut(s)	1424, 1448, 1670, 2165, 2362
AcyI	GRCGYC	1 cut(s)	661
AdeI	CACNNNGTG	1 cut(s)	879
AfaI	GTAC	5 cut(s)	74, 190, 1398, 1476, 1708
AfiI	CCNNNNNNNGG	3 cut(s)	244, 772, 1590
AflIII	ACRYGT	1 cut(s)	312
AhlI	ACTAGT	1 cut(s)	1000
AjiI	CACGTC	1 cut(s)	51
AjnI	CCWGG	3 cut(s)	179, 768, 1946
AleI	CACNNNNGTG	1 cut(s)	1962
Alw21I	GWGCWC	1 cut(s)	929
Alw26I	GTCTC	1 cut(s)	779
AlwI	GGATC	6 cut(s)	716, 1276, 1662, 1806, 2175, 2601
AlwNI	CAGNNNCTG	1 cut(s)	2182
Ama87I	CYCGRG	1 cut(s)	2471
Aor13HI	TCCGGA	1 cut(s)	935
AoxI	GGCC	8 cut(s)	235, 269, 282, 510, 652, 882, 984, 2268
ApeKI	GCWGC	8 cut(s)	111, 140, 1721, 1730, 2386, 2404, 2456, 2548
ApoI	RAATTY	5 cut(s)	356, 515, 944, 1244, 1554
ArsI	GACNNNNNNTTYG	2 cut(s)	2096, 2128
Asp700I	GAANNNNTTC	1 cut(s)	1047
AspLEI	GCGC	3 cut(s)	44, 1985, 2249
AsuC2I	CCSGG	5 cut(s)	674, 691, 877, 2472, 2473
AsuII	TTCGAA	1 cut(s)	2444
AsuNHI	GCTAGC	2 cut(s)	1259, 2044
AvaI	CYCGRG	1 cut(s)	2471
AvaII	GGWCC	5 cut(s)	218, 332, 1808, 2140, 2315
AxyI	CCTNAGG	1 cut(s)	656
BaeGI	GKGCMC	2 cut(s)	2416, 2471
BaeI	ACNNNNGTAYC	2 cut(s)	1818, 1851
BanI	GGYRCC	1 cut(s)	457
BanII	GRGCYC	2 cut(s)	179, 929
BauI	CACGAG	1 cut(s)	2017
BbsI	GAAGAC	1 cut(s)	2510
Bbv12I	GWGCWC	1 cut(s)	929
BbvCI	CCTCAGC	1 cut(s)	2502
BbvI	GCAGC	8 cut(s)	98, 127, 1708, 1717, 2398, 2416, 2443, 2560
BcgI	CGANNNNNNTGC	2 cut(s)	33, 67
BciT130I	CCWGG	3 cut(s)	181, 770, 1948
BciVI	GTATCC	1 cut(s)	2213
BclI	TGATCA	1 cut(s)	2152
BcnI	CCSGG	5 cut(s)	674, 691, 877, 2472, 2473
BcoDI	GTCTC	1 cut(s)	779
BcuI	ACTAGT	1 cut(s)	1000
BfaI	CTAG	7 cut(s)	1001, 1260, 1785, 2045, 2051, 2240, 2294
BfmI	CTRYAG	5 cut(s)	8, 537, 635, 1347, 2055
BfoI	RGCGCY	2 cut(s)	1986, 2250
BfuAI	ACCTGC	1 cut(s)	628
BfuI	GTATCC	1 cut(s)	2213
Bme1390I	CCNGG	8 cut(s)	181, 674, 691, 770, 877, 1948, 2472, 2473
Bme18I	GGWCC	5 cut(s)	218, 332, 1808, 2140, 2315
BmeT110I	CYCGRG	1 cut(s)	2471
BmgBI	CACGTC	1 cut(s)	51
BmiI	GGNNCC	7 cut(s)	178, 220, 351, 459, 511, 934, 1615
BmrFI	CCNGG	8 cut(s)	181, 674, 691, 770, 877, 1948, 2472, 2473
BmrI	ACTGGG	1 cut(s)	96
BmsI	GCATC	8 cut(s)	53, 532, 1153, 1327, 1478, 2359, 2443, 2468
BmtI	GCTAGC	2 cut(s)	1263, 2048
BmuI	ACTGGG	1 cut(s)	96
BoxI	GACNNNNGTC	2 cut(s)	302, 791
BpiI	GAAGAC	1 cut(s)	2510
Bpu10I	CCTNAGC	1 cut(s)	2502
Bpu14I	TTCGAA	1 cut(s)	2444
BpuEI	CTTGAG	3 cut(s)	1151, 1517, 2528
BpuMI	CCSGG	5 cut(s)	674, 691, 877, 2472, 2473
BsaAI	YACGTR	1 cut(s)	1607
BsaBI	GATNNNNATC	1 cut(s)	698
BsaHI	GRCGYC	1 cut(s)	661
BsaI	GGTCTC	1 cut(s)	779
BsaWI	WCCGGW	1 cut(s)	935
Bsc4I	CCNNNNNNNGG	3 cut(s)	244, 772, 1590
Bse118I	RCCGGY	1 cut(s)	287
Bse1I	ACTGG	2 cut(s)	102, 1332
Bse21I	CCTNAGG	1 cut(s)	656
Bse3DI	GCAATG	1 cut(s)	2543
Bse8I	GATNNNNATC	1 cut(s)	698
BseAI	TCCGGA	1 cut(s)	935
BseBI	CCWGG	3 cut(s)	181, 770, 1948
BseGI	GGATG	4 cut(s)	44, 916, 1390, 1414
BseJI	GATNNNNATC	1 cut(s)	698
BseLI	CCNNNNNNNGG	3 cut(s)	244, 772, 1590
BseMI	GCAATG	1 cut(s)	2543
BseMII	CTCAG	3 cut(s)	647, 1977, 2516
BseNI	ACTGG	2 cut(s)	102, 1332
BseRI	GAGGAG	4 cut(s)	18, 79, 82, 1107
BseSI	GKGCMC	2 cut(s)	2416, 2471
BseXI	GCAGC	8 cut(s)	98, 127, 1708, 1717, 2398, 2416, 2443, 2560
Bsh1236I	CGCG	1 cut(s)	2602
Bsh1285I	CGRYCG	1 cut(s)	706
BshFI	GGCC	8 cut(s)	237, 271, 284, 512, 654, 884, 986, 2270
BshNI	GGYRCC	1 cut(s)	457
BsiEI	CGRYCG	1 cut(s)	706
BsiHKAI	GWGCWC	1 cut(s)	929
BsiHKCI	CYCGRG	1 cut(s)	2471
BsiSI	CCGG	6 cut(s)	288, 673, 690, 877, 936, 2472
BslFI	GGGAC	1 cut(s)	204
BslI	CCNNNNNNNGG	3 cut(s)	244, 772, 1590
BsmAI	GTCTC	1 cut(s)	779
BsmFI	GGGAC	1 cut(s)	204
BsmI	GAATGC	1 cut(s)	139
BsnI	GGCC	8 cut(s)	237, 271, 284, 512, 654, 884, 986, 2270
Bso31I	GGTCTC	1 cut(s)	779
BsoBI	CYCGRG	1 cut(s)	2471
Bsp119I	TTCGAA	1 cut(s)	2444
Bsp1286I	GDGCHC	4 cut(s)	179, 929, 2416, 2471
Bsp13I	TCCGGA	1 cut(s)	935
Bsp143I	GATC	9 cut(s)	78, 551, 721, 1268, 1654, 1798, 2152, 2167, 2593
BspANI	GGCC	8 cut(s)	237, 271, 284, 512, 654, 884, 986, 2270
BspCNI	CTCAG	3 cut(s)	648, 1978, 2515
BspEI	TCCGGA	1 cut(s)	935
BspFNI	CGCG	1 cut(s)	2602
BspHI	TCATGA	1 cut(s)	1558
BspLI	GGNNCC	7 cut(s)	178, 220, 351, 459, 511, 934, 1615
BspMAI	CTGCAG	2 cut(s)	12, 639
BspMI	ACCTGC	1 cut(s)	628
BspOI	GCTAGC	2 cut(s)	1263, 2048
BspPI	GGATC	6 cut(s)	716, 1276, 1662, 1806, 2175, 2601
BspQI	GCTCTTC	1 cut(s)	2609
BspT104I	TTCGAA	1 cut(s)	2444
BspT107I	GGYRCC	1 cut(s)	457
BspTNI	GGTCTC	1 cut(s)	779
BsrDI	GCAATG	1 cut(s)	2543
BsrFI	RCCGGY	1 cut(s)	287
BsrI	ACTGG	2 cut(s)	102, 1332
BssAI	RCCGGY	1 cut(s)	287
BssMI	GATC	9 cut(s)	78, 551, 721, 1268, 1654, 1798, 2152, 2167, 2593
BssNAI	GTATAC	1 cut(s)	318
BssNI	GRCGYC	1 cut(s)	661
BssSI	CACGAG	1 cut(s)	2017
BssT1I	CCWWGG	4 cut(s)	905, 1231, 1639, 2007
Bst1107I	GTATAC	1 cut(s)	318
Bst2BI	CACGAG	1 cut(s)	2017
Bst2UI	CCWGG	3 cut(s)	181, 770, 1948
Bst6I	CTCTTC	2 cut(s)	2083, 2609
BstACI	GRCGYC	1 cut(s)	661
BstBAI	YACGTR	1 cut(s)	1607
BstBI	TTCGAA	1 cut(s)	2444
BstDEI	CTNAG	3 cut(s)	656, 1986, 2502
BstDSI	CCRYGG	2 cut(s)	2526, 2600
BstEII	GGTNACC	1 cut(s)	1097
BstF5I	GGATG	4 cut(s)	44, 916, 1390, 1414
BstFNI	CGCG	1 cut(s)	2602
BstH2I	RGCGCY	2 cut(s)	1986, 2250
BstHHI	GCGC	3 cut(s)	44, 1985, 2249
BstKTI	GATC	9 cut(s)	81, 554, 724, 1271, 1657, 1801, 2155, 2170, 2596
BstMAI	GTCTC	1 cut(s)	779
BstMBI	GATC	9 cut(s)	78, 551, 721, 1268, 1654, 1798, 2152, 2167, 2593
BstMCI	CGRYCG	1 cut(s)	706
BstMWI	GCNNNNNNNGC	7 cut(s)	39, 542, 866, 1215, 1727, 1730, 2045
BstNI	CCWGG	3 cut(s)	181, 770, 1948
BstNSI	RCATGY	2 cut(s)	316, 1313
BstPAI	GACNNNNGTC	2 cut(s)	302, 791
BstPI	GGTNACC	1 cut(s)	1097
BstSCI	CCNGG	8 cut(s)	179, 672, 689, 768, 875, 1946, 2470, 2471
BstSFI	CTRYAG	5 cut(s)	8, 537, 635, 1347, 2055
BstSLI	GKGCMC	2 cut(s)	2416, 2471
BstUI	CGCG	1 cut(s)	2602
BstV1I	GCAGC	8 cut(s)	98, 127, 1708, 1717, 2398, 2416, 2443, 2560
BstV2I	GAAGAC	1 cut(s)	2510
BstX2I	RGATCY	1 cut(s)	2167
BstXI	CCANNNNNNTGG	1 cut(s)	329
BstYI	RGATCY	1 cut(s)	2167
BstZ17I	GTATAC	1 cut(s)	318
Bsu36I	CCTNAGG	1 cut(s)	656
BsuI	GTATCC	1 cut(s)	2213
BsuRI	GGCC	8 cut(s)	237, 271, 284, 512, 654, 884, 986, 2270
BtgI	CCRYGG	2 cut(s)	2526, 2600
BtrI	CACGTC	1 cut(s)	51
BtsCI	GGATG	4 cut(s)	44, 916, 1390, 1414
BtsI	GCAGTG	1 cut(s)	1957
BtsIMutI	CAGTG	7 cut(s)	210, 222, 1339, 1957, 2287, 2469, 2568
BveI	ACCTGC	1 cut(s)	628
CaiI	CAGNNNCTG	1 cut(s)	2182
CciI	TCATGA	1 cut(s)	1558
CfoI	GCGC	3 cut(s)	44, 1985, 2249
Cfr10I	RCCGGY	1 cut(s)	287
Cfr42I	CCGCGG	1 cut(s)	2603
Cfr9I	CCCGGG	1 cut(s)	2471
CseI	GACGC	1 cut(s)	650
Csp6I	GTAC	5 cut(s)	73, 189, 1397, 1475, 1707
CviAII	CATG	7 cut(s)	313, 857, 890, 1310, 1448, 1559, 2312
CviQI	GTAC	5 cut(s)	73, 189, 1397, 1475, 1707
DdeI	CTNAG	3 cut(s)	656, 1986, 2502
DpnI	GATC	9 cut(s)	80, 553, 723, 1270, 1656, 1800, 2154, 2169, 2595
DpnII	GATC	9 cut(s)	78, 551, 721, 1268, 1654, 1798, 2152, 2167, 2593
DraI	TTTAAA	1 cut(s)	1174
DraIII	CACNNNGTG	1 cut(s)	879
DrdI	GACNNNNNNGTC	1 cut(s)	299
DseDI	GACNNNNNNGTC	1 cut(s)	299
Eam1104I	CTCTTC	2 cut(s)	2083, 2609
EarI	CTCTTC	2 cut(s)	2083, 2609
EciI	GGCGGA	3 cut(s)	55, 676, 755
Ecl136II	GAGCTC	1 cut(s)	927
Eco130I	CCWWGG	4 cut(s)	905, 1231, 1639, 2007
Eco147I	AGGCCT	1 cut(s)	986
Eco24I	GRGCYC	2 cut(s)	179, 929
Eco31I	GGTCTC	1 cut(s)	779
Eco47I	GGWCC	5 cut(s)	218, 332, 1808, 2140, 2315
Eco53kI	GAGCTC	1 cut(s)	927
Eco57I	CTGAAG	5 cut(s)	1424, 1448, 1670, 2165, 2362
Eco81I	CCTNAGG	1 cut(s)	656
Eco88I	CYCGRG	1 cut(s)	2471
Eco91I	GGTNACC	1 cut(s)	1097
EcoICRI	GAGCTC	1 cut(s)	927
EcoO109I	RGGNCCY	2 cut(s)	1808, 2140
EcoO65I	GGTNACC	1 cut(s)	1097
EcoRII	CCWGG	3 cut(s)	179, 768, 1946
EcoT14I	CCWWGG	4 cut(s)	905, 1231, 1639, 2007
EcoT38I	GRGCYC	2 cut(s)	179, 929
ErhI	CCWWGG	4 cut(s)	905, 1231, 1639, 2007
FaeI	CATG	7 cut(s)	316, 860, 893, 1313, 1451, 1562, 2315
FalI	AAGNNNNNCTT	6 cut(s)	248, 280, 1113, 1145, 2256, 2288
FaqI	GGGAC	1 cut(s)	204
FatI	CATG	7 cut(s)	312, 856, 889, 1309, 1447, 1558, 2311
FauI	CCCGC	2 cut(s)	753, 2290
FauNDI	CATATG	1 cut(s)	1159
FbaI	TGATCA	1 cut(s)	2152
FblI	GTMKAC	4 cut(s)	252, 317, 804, 2425
FokI	GGATG	4 cut(s)	31, 903, 1397, 1421
FriOI	GRGCYC	2 cut(s)	179, 929
FspBI	CTAG	7 cut(s)	1001, 1260, 1785, 2045, 2051, 2240, 2294
GlaI	GCGC	3 cut(s)	43, 1984, 2248
HaeII	RGCGCY	2 cut(s)	1986, 2250
HaeIII	GGCC	8 cut(s)	237, 271, 284, 512, 654, 884, 986, 2270
HapII	CCGG	6 cut(s)	288, 673, 690, 877, 936, 2472
HgaI	GACGC	1 cut(s)	650
HhaI	GCGC	3 cut(s)	44, 1985, 2249
Hin1I	GRCGYC	1 cut(s)	661
Hin1II	CATG	7 cut(s)	316, 860, 893, 1313, 1451, 1562, 2315
Hin6I	GCGC	3 cut(s)	42, 1983, 2247
HinP1I	GCGC	3 cut(s)	42, 1983, 2247
HincII	GTYRAC	3 cut(s)	253, 613, 2426
HindII	GTYRAC	3 cut(s)	253, 613, 2426
HindIII	AAGCTT	3 cut(s)	401, 1055, 2147
HpaII	CCGG	6 cut(s)	288, 673, 690, 877, 936, 2472
Hpy188I	TCNGA	7 cut(s)	574, 600, 1030, 1404, 1872, 2135, 2157
Hpy99I	CGWCG	3 cut(s)	55, 305, 524
HpyCH4IV	ACGT	4 cut(s)	50, 609, 1606, 2589
HpyF10VI	GCNNNNNNNGC	7 cut(s)	39, 542, 866, 1215, 1727, 1730, 2045
HpyF3I	CTNAG	3 cut(s)	656, 1986, 2502
HpySE526I	ACGT	4 cut(s)	50, 609, 1606, 2589
Hsp92I	GRCGYC	1 cut(s)	661
Hsp92II	CATG	7 cut(s)	316, 860, 893, 1313, 1451, 1562, 2315
HspAI	GCGC	3 cut(s)	42, 1983, 2247
Kpn2I	TCCGGA	1 cut(s)	935
KroI	GCCGGC	1 cut(s)	287
KroNI	GCCGGC	1 cut(s)	289
Ksp22I	TGATCA	1 cut(s)	2152
KspI	CCGCGG	1 cut(s)	2603
Kzo9I	GATC	9 cut(s)	78, 551, 721, 1268, 1654, 1798, 2152, 2167, 2593
LguI	GCTCTTC	1 cut(s)	2609
LmnI	GCTCC	9 cut(s)	38, 68, 92, 103, 182, 355, 439, 907, 1233
Lsp1109I	GCAGC	8 cut(s)	98, 127, 1708, 1717, 2398, 2416, 2443, 2560
LweI	GCATC	8 cut(s)	53, 532, 1153, 1327, 1478, 2359, 2443, 2468
MaeI	CTAG	7 cut(s)	1001, 1260, 1785, 2045, 2051, 2240, 2294
MaeII	ACGT	4 cut(s)	50, 609, 1606, 2589
MaeIII	GTNAC	9 cut(s)	241, 410, 626, 1097, 1529, 1768, 2063, 2196, 2620
MalI	GATC	9 cut(s)	80, 553, 723, 1270, 1656, 1800, 2154, 2169, 2595
MboI	GATC	9 cut(s)	78, 551, 721, 1268, 1654, 1798, 2152, 2167, 2593
MboII	GAAGA	9 cut(s)	142, 181, 732, 851, 1760, 1835, 2100, 2510, 2626
MflI	RGATCY	1 cut(s)	2167
MhlI	GDGCHC	4 cut(s)	179, 929, 2416, 2471
MlyI	GAGTC	3 cut(s)	975, 2029, 2421
MmeI	TCCRAC	2 cut(s)	127, 1776
MreI	CGCCGGCG	1 cut(s)	287
MroI	TCCGGA	1 cut(s)	935
MroNI	GCCGGC	1 cut(s)	287
MroXI	GAANNNNTTC	1 cut(s)	1047
MseI	TTAA	8 cut(s)	830, 989, 1173, 1248, 1662, 2532, 2576, 2586
MslI	CAYNNNNRTG	4 cut(s)	1962, 1974, 2435, 2464
MspA1I	CMGCKG	3 cut(s)	1832, 2551, 2602
MspI	CCGG	6 cut(s)	288, 673, 690, 877, 936, 2472
MspR9I	CCNGG	8 cut(s)	181, 674, 691, 770, 877, 1948, 2472, 2473
Mva1269I	GAATGC	1 cut(s)	139
MvaI	CCWGG	3 cut(s)	181, 770, 1948
MvnI	CGCG	1 cut(s)	2602
MwoI	GCNNNNNNNGC	7 cut(s)	39, 542, 866, 1215, 1727, 1730, 2045
NaeI	GCCGGC	1 cut(s)	289
NciI	CCSGG	5 cut(s)	674, 691, 877, 2472, 2473
NdeI	CATATG	1 cut(s)	1159
NdeII	GATC	9 cut(s)	78, 551, 721, 1268, 1654, 1798, 2152, 2167, 2593
NgoMIV	GCCGGC	1 cut(s)	287
NheI	GCTAGC	2 cut(s)	1259, 2044
NlaIII	CATG	7 cut(s)	316, 860, 893, 1313, 1451, 1562, 2315
NlaIV	GGNNCC	7 cut(s)	178, 220, 351, 459, 511, 934, 1615
NmuCI	GTSAC	6 cut(s)	241, 1097, 1768, 2063, 2196, 2620
NspI	RCATGY	2 cut(s)	316, 1313
NspV	TTCGAA	1 cut(s)	2444
OliI	CACNNNNGTG	1 cut(s)	1962
PagI	TCATGA	1 cut(s)	1558
PaqCI	CACCTGC	1 cut(s)	628
PceI	AGGCCT	1 cut(s)	986
PciI	ACATGT	1 cut(s)	312
PciSI	GCTCTTC	1 cut(s)	2609
PcsI	WCGNNNNNNNCGW	3 cut(s)	197, 789, 798
PctI	GAATGC	1 cut(s)	139
PdiI	GCCGGC	1 cut(s)	289
PdmI	GAANNNNTTC	1 cut(s)	1047
PfeI	GAWTC	7 cut(s)	683, 842, 995, 1014, 1025, 1571, 2605
PflFI	GACNNNGTC	1 cut(s)	212
PleI	GAGTC	3 cut(s)	974, 2028, 2421
PpsI	GAGTC	3 cut(s)	974, 2028, 2421
Ppu21I	YACGTR	1 cut(s)	1607
PpuMI	RGGWCCY	2 cut(s)	1808, 2140
PscI	ACATGT	1 cut(s)	312
PshAI	GACNNNNGTC	2 cut(s)	302, 791
Psp124BI	GAGCTC	1 cut(s)	929
Psp5II	RGGWCCY	2 cut(s)	1808, 2140
Psp6I	CCWGG	3 cut(s)	179, 768, 1946
PspEI	GGTNACC	1 cut(s)	1097
PspGI	CCWGG	3 cut(s)	179, 768, 1946
PspN4I	GGNNCC	7 cut(s)	178, 220, 351, 459, 511, 934, 1615
PspPPI	RGGWCCY	2 cut(s)	1808, 2140
PstI	CTGCAG	2 cut(s)	12, 639
PstNI	CAGNNNCTG	1 cut(s)	2182
PsuI	RGATCY	1 cut(s)	2167
PsyI	GACNNNGTC	1 cut(s)	212
PvuII	CAGCTG	2 cut(s)	1832, 2551
RsaI	GTAC	5 cut(s)	74, 190, 1398, 1476, 1708
RsaNI	GTAC	5 cut(s)	73, 189, 1397, 1475, 1707
RseI	CAYNNNNRTG	4 cut(s)	1962, 1974, 2435, 2464
SacI	GAGCTC	1 cut(s)	929
SacII	CCGCGG	1 cut(s)	2603
SalI	GTCGAC	2 cut(s)	251, 2424
SapI	GCTCTTC	1 cut(s)	2609
SaqAI	TTAA	8 cut(s)	830, 989, 1173, 1248, 1662, 2532, 2576, 2586
Sau3AI	GATC	9 cut(s)	78, 551, 721, 1268, 1654, 1798, 2152, 2167, 2593
SchI	GAGTC	3 cut(s)	975, 2029, 2421
ScrFI	CCNGG	8 cut(s)	181, 674, 691, 770, 877, 1948, 2472, 2473
SduI	GDGCHC	4 cut(s)	179, 929, 2416, 2471
SfaNI	GCATC	8 cut(s)	53, 532, 1153, 1327, 1478, 2359, 2443, 2468
SfcI	CTRYAG	5 cut(s)	8, 537, 635, 1347, 2055
Sfr303I	CCGCGG	1 cut(s)	2603
SfuI	TTCGAA	1 cut(s)	2444
SgrAI	CRCCGGYG	1 cut(s)	287
SgrBI	CCGCGG	1 cut(s)	2603
SinI	GGWCC	5 cut(s)	218, 332, 1808, 2140, 2315
SmaI	CCCGGG	1 cut(s)	2473
SmiMI	CAYNNNNRTG	4 cut(s)	1962, 1974, 2435, 2464
SmlI	CTYRAG	3 cut(s)	1130, 1496, 2507
SmoI	CTYRAG	3 cut(s)	1130, 1496, 2507
SpeI	ACTAGT	1 cut(s)	1000
SseBI	AGGCCT	1 cut(s)	986
SspI	AATATT	1 cut(s)	2536
SspMI	CTAG	7 cut(s)	1001, 1260, 1785, 2045, 2051, 2240, 2294
SstI	GAGCTC	1 cut(s)	929
StuI	AGGCCT	1 cut(s)	986
StyD4I	CCNGG	8 cut(s)	179, 672, 689, 768, 875, 1946, 2470, 2471
StyI	CCWWGG	4 cut(s)	905, 1231, 1639, 2007
TaiI	ACGT	4 cut(s)	53, 612, 1609, 2592
TaqII	GACCGA	1 cut(s)	206
TatI	WGTACW	1 cut(s)	1474
TauI	GCSGC	4 cut(s)	20, 140, 287, 2602
TfiI	GAWTC	7 cut(s)	683, 842, 995, 1014, 1025, 1571, 2605
Tru1I	TTAA	8 cut(s)	830, 989, 1173, 1248, 1662, 2532, 2576, 2586
Tru9I	TTAA	8 cut(s)	830, 989, 1173, 1248, 1662, 2532, 2576, 2586
TscAI	CASTG	7 cut(s)	217, 229, 1339, 1964, 2287, 2469, 2575
TseFI	GTSAC	6 cut(s)	241, 1097, 1768, 2063, 2196, 2620
TseI	GCWGC	8 cut(s)	111, 140, 1721, 1730, 2386, 2404, 2456, 2548
Tsp45I	GTSAC	6 cut(s)	241, 1097, 1768, 2063, 2196, 2620
TspDTI	ATGAA	8 cut(s)	957, 1547, 1575, 1684, 1958, 2177, 2321, 2349
TspGWI	ACGGA	4 cut(s)	260, 790, 1026, 1212
TspMI	CCCGGG	1 cut(s)	2471
TspRI	CASTG	7 cut(s)	217, 229, 1339, 1964, 2287, 2469, 2575
Tth111I	GACNNNGTC	1 cut(s)	212
VpaK11BI	GGWCC	5 cut(s)	218, 332, 1808, 2140, 2315
XapI	RAATTY	5 cut(s)	356, 515, 944, 1244, 1554
XbaI	TCTAGA	1 cut(s)	2239
XceI	RCATGY	2 cut(s)	316, 1313
XcmI	CCANNNNNNNNNTGG	2 cut(s)	229, 1498
XmaI	CCCGGG	1 cut(s)	2471
XmiI	GTMKAC	4 cut(s)	252, 317, 804, 2425
XmnI	GAANNNNTTC	1 cut(s)	1047
XspI	CTAG	7 cut(s)	1001, 1260, 1785, 2045, 2051, 2240, 2294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.