pycom04g21710

protein KRI1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009859
Physical Location & Seq
Forward (+)
22734787 .. 22736355
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1362 bp
ATGGATGCCTTGAGAAGAAATAAAAGTGATTTTCATTTTGCCTCCATTTCGGTCCTAAATAAAAAAAAATATATAAAAGGGGAGTCCTTTTCAAGTTCCAAACTAAAAACCCCCGCCACCGCCTCAGCCCCCAAATTAATTGAAGAAAAAGAAGCAGCAGCGACTCAGATAGAAATTGAGGAAGAAGAAGCAGCAGCAACTCAGATATTCGGCGGAAACAATATGGGCGGGCTTCAACTACTCGACAGCGGAAGCGACTCCGAGAACGAAAACGGAAACGACGACGTTCCGCAGCTCAAAATCAATGAAGATTTCGCTCGCAGGTACGAACACAACAAGAAGCGGGAGGAGCTCCAACGCTACGAGGAACTGAAGAAGCGAGGCCTCGTGGCTGACCCCTCCGAAGATTCAGACTCTGAACCAGATTTTGAATCGGACGACGACTATGTCCATGTGGCGAATTCCAAGAAGAGGGAATTGGAATTTTTGGATGGTCTGCTCAAGGTAAAAAAGCAAGACCCTATCTTGAAAAACAAAGATGTTGTTTTGTTTAATTCTGATGGAACCAAAATTATTGGAAACAATGGAGGAGAGGGAAAGGCAAAGAATAAGAAGAAGATGTACTTGAAAGATGTTAATGCTAAGCATTTGATGGAGGATGGCCCGGAACTGGCGGAGGAGGATGGCAAGAACATTGATAAAAACAGTAAGGTTTACAACGAAGAGCAAGAGCGGATAAGGAGAGAATTCTTACAGGCCGCTGCAGCCGAGGAGGATGATGATGATGGTGAGTTTTTGATAGAGAAGAATAAGAATGCTGCGGGGGATGATGATGATGATGATGATGATACTGTTGATAATGATCAGTATGAGAAGGCATTGGATGAGTGTTTCCCTGAATCGGATGAAAATGCTATGTTTCTCAAGAAGTTCTTTAAGGATCAGCTGTGGAAGGAGGATAAGGATCCCGAGCTAAAGGAGGAGGATTTGGAGATGGTGTCCGAGGACGAGATGGAGATCGAAAGGCAGGAGGAGTATGAGTACAGGTTCCAGGAGAATGCAGGTGATCGGATTATGGGGCATTCCAGGCAAGTTGAGGGGTCGGTGAGGAAGAAGGTCAAGGCGAGGAAGGAGCAGAGGAAGAGCAAGGAGGAGAGGATGGAGATTGCAAGGTTGGAGAGGGAGGAGGAGTTGAGGCATTTGAAGAATTTGAAGAAGAAGGAGAACGACGAGAAGGTTAAGAAGATAATGGAGATTGCGGGGCTTAAGGAGGGCGAGGAGTCTACATTTGATCCAAAGGAGTTGGAGAAGGAATATGATCCCCAAGAATATCGATCGGATGATGAAGAAGGCTTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kri1 Kri1_C

Protein Analysis

454

Amino Acids

52.52

Weight (kDa)

4.68

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kri1 PF05178 385 - 445 3.8e-07 KRI1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1052
AasI GACNNNNNNGTC 1 cut(s) 446
Acc36I ACCTGC 2 cut(s) 312, 1052
AccBSI CCGCTC 1 cut(s) 733
AccI GTMKAC 1 cut(s) 1283
AclWI GGATC 5 cut(s) 948, 959, 972, 1286, 1313
AcsI RAATTY 4 cut(s) 460, 482, 746, 1207
AcuI CTGAAG 1 cut(s) 392
AfaI GTAC 3 cut(s) 326, 623, 1043
AfiI CCNNNNNNNGG 3 cut(s) 471, 670, 901
AflII CTTAAG 1 cut(s) 1265
AgsI TTSAA 8 cut(s) 93, 143, 236, 431, 529, 628, 1204, 1213
AjnI CCWGG 2 cut(s) 1050, 1085
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 4 cut(s) 295, 352, 946, 973
AluI AGCT 4 cut(s) 295, 352, 946, 973
Alw21I GWGCWC 1 cut(s) 354
AlwI GGATC 5 cut(s) 948, 959, 972, 1286, 1313
AlwNI CAGNNNCTG 1 cut(s) 416
Ama87I CYCGRG 1 cut(s) 968
AoxI GGCC 3 cut(s) 382, 661, 756
ApeKI GCWGC 8 cut(s) 155, 158, 191, 194, 292, 761, 764, 818
ApoI RAATTY 4 cut(s) 460, 482, 746, 1207
AseI ATTAAT 1 cut(s) 137
AspS9I GGNCC 2 cut(s) 52, 662
AsuC2I CCSGG 1 cut(s) 665
AsuHPI GGTGA 3 cut(s) 800, 1076, 1117
AvaI CYCGRG 1 cut(s) 968
AvaII GGWCC 1 cut(s) 52
BamHI GGATCC 1 cut(s) 964
BanII GRGCYC 1 cut(s) 354
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BauI CACGAG 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 354
BbvCI CCTCAGC 1 cut(s) 124
BbvI GCAGC 8 cut(s) 167, 170, 203, 206, 304, 748, 776, 805
BccI CCATC 9 cut(s) 485, 554, 646, 653, 677, 779, 988, 1006, 1153
BciT130I CCWGG 2 cut(s) 1052, 1087
BclI TGATCA 1 cut(s) 862
BcnI CCSGG 1 cut(s) 665
BfmI CTRYAG 1 cut(s) 762
BfrI CTTAAG 1 cut(s) 1265
BfuAI ACCTGC 2 cut(s) 312, 1052
BisI GCNGC 9 cut(s) 156, 159, 192, 195, 293, 759, 762, 765, 819
BlpI GCTNAGC 1 cut(s) 642
BlsI GCNGC 9 cut(s) 157, 160, 193, 196, 294, 760, 763, 766, 820
Bme1390I CCNGG 3 cut(s) 665, 1052, 1087
Bme18I GGWCC 1 cut(s) 52
BmeT110I CYCGRG 1 cut(s) 968
BmgT120I GGNCC 2 cut(s) 52, 662
BmiI GGNNCC 3 cut(s) 565, 966, 1049
BmrFI CCNGG 3 cut(s) 665, 1052, 1087
Bpu10I CCTNAGC 1 cut(s) 124
Bpu1102I GCTNAGC 1 cut(s) 642
BpuEI CTTGAG 3 cut(s) 31, 485, 908
BpuMI CCSGG 1 cut(s) 665
Bsa29I ATCGAT 1 cut(s) 1333
BsaBI GATNNNNATC 3 cut(s) 861, 963, 1016
BsaJI CCNNGG 2 cut(s) 768, 1002
BsaXI ACNNNNNCTCC 6 cut(s) 983, 1013, 1025, 1055, 1175, 1205
Bsc4I CCNNNNNNNGG 3 cut(s) 471, 670, 901
Bse1I ACTGG 1 cut(s) 675
Bse8I GATNNNNATC 3 cut(s) 861, 963, 1016
BseBI CCWGG 2 cut(s) 1052, 1087
BseCI ATCGAT 1 cut(s) 1333
BseDI CCNNGG 2 cut(s) 768, 1002
BseJI GATNNNNATC 3 cut(s) 861, 963, 1016
BseLI CCNNNNNNNGG 3 cut(s) 471, 670, 901
BseMII CTCAG 3 cut(s) 138, 179, 215
BseNI ACTGG 1 cut(s) 675
BseXI GCAGC 8 cut(s) 167, 170, 203, 206, 304, 748, 776, 805
Bsh1285I CGRYCG 1 cut(s) 1337
BshFI GGCC 3 cut(s) 384, 663, 758
BshVI ATCGAT 1 cut(s) 1333
BsiEI CGRYCG 1 cut(s) 1337
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 968
BsiSI CCGG 1 cut(s) 665
BslI CCNNNNNNNGG 3 cut(s) 471, 670, 901
BsmI GAATGC 3 cut(s) 820, 1063, 1081
BsnI GGCC 3 cut(s) 384, 663, 758
BsoBI CYCGRG 1 cut(s) 968
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 8 cut(s) 862, 940, 964, 1017, 1066, 1291, 1318, 1334
Bsp1720I GCTNAGC 1 cut(s) 642
BspANI GGCC 3 cut(s) 384, 663, 758
BspCNI CTCAG 3 cut(s) 137, 178, 214
BspDI ATCGAT 1 cut(s) 1333
BspLI GGNNCC 3 cut(s) 565, 966, 1049
BspMAI CTGCAG 1 cut(s) 766
BspMI ACCTGC 2 cut(s) 312, 1052
BspPI GGATC 5 cut(s) 948, 959, 972, 1286, 1313
BspQI GCTCTTC 2 cut(s) 717, 1136
BspTI CTTAAG 1 cut(s) 1265
BsrBI CCGCTC 1 cut(s) 733
BsrI ACTGG 1 cut(s) 675
BssECI CCNNGG 2 cut(s) 768, 1002
BssMI GATC 8 cut(s) 862, 940, 964, 1017, 1066, 1291, 1318, 1334
BssSI CACGAG 1 cut(s) 386
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 2 cut(s) 1052, 1087
Bst4CI ACNGT 2 cut(s) 707, 853
Bst6I CTCTTC 3 cut(s) 464, 717, 1136
BstAFI CTTAAG 1 cut(s) 1265
BstC8I GCNNGC 2 cut(s) 230, 319
BstDEI CTNAG 4 cut(s) 124, 165, 201, 642
BstKTI GATC 8 cut(s) 865, 943, 967, 1020, 1069, 1294, 1321, 1337
BstMBI GATC 8 cut(s) 862, 940, 964, 1017, 1066, 1291, 1318, 1334
BstMCI CGRYCG 1 cut(s) 1337
BstMWI GCNNNNNNNGC 3 cut(s) 349, 764, 1087
BstNI CCWGG 2 cut(s) 1052, 1087
BstSCI CCNGG 3 cut(s) 663, 1050, 1085
BstSFI CTRYAG 1 cut(s) 762
BstV1I GCAGC 8 cut(s) 167, 170, 203, 206, 304, 748, 776, 805
BstX2I RGATCY 1 cut(s) 964
BstYI RGATCY 1 cut(s) 964
Bsu15I ATCGAT 1 cut(s) 1333
BsuRI GGCC 3 cut(s) 384, 663, 758
BsuTUI ATCGAT 1 cut(s) 1333
BveI ACCTGC 2 cut(s) 312, 1052
Cac8I GCNNGC 2 cut(s) 230, 319
CaiI CAGNNNCTG 1 cut(s) 416
Cfr13I GGNCC 2 cut(s) 52, 662
ClaI ATCGAT 1 cut(s) 1333
Csp6I GTAC 3 cut(s) 325, 622, 1042
CviAII CATG 1 cut(s) 452
CviQI GTAC 3 cut(s) 325, 622, 1042
DdeI CTNAG 4 cut(s) 124, 165, 201, 642
DpnI GATC 8 cut(s) 864, 942, 966, 1019, 1068, 1293, 1320, 1336
DpnII GATC 8 cut(s) 862, 940, 964, 1017, 1066, 1291, 1318, 1334
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
Eam1104I CTCTTC 3 cut(s) 464, 717, 1136
EarI CTCTTC 3 cut(s) 464, 717, 1136
EciI GGCGGA 2 cut(s) 228, 689
Ecl136II GAGCTC 1 cut(s) 352
Eco147I AGGCCT 1 cut(s) 384
Eco24I GRGCYC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 968
EcoICRI GAGCTC 1 cut(s) 352
EcoRI GAATTC 2 cut(s) 460, 746
EcoRII CCWGG 2 cut(s) 1050, 1085
EcoT38I GRGCYC 1 cut(s) 354
FaeI CATG 1 cut(s) 455
FalI AAGNNNNNCTT 4 cut(s) 608, 640, 917, 949
FatI CATG 1 cut(s) 451
FauI CCCGC 5 cut(s) 121, 221, 336, 814, 1252
FbaI TGATCA 1 cut(s) 862
FblI GTMKAC 1 cut(s) 1283
Fnu4HI GCNGC 9 cut(s) 156, 159, 192, 195, 293, 759, 762, 765, 819
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 9 cut(s) 156, 159, 192, 195, 293, 759, 762, 765, 819
GluI GCNGC 9 cut(s) 156, 159, 192, 195, 293, 759, 762, 765, 819
HaeIII GGCC 3 cut(s) 384, 663, 758
HapII CCGG 1 cut(s) 665
Hin1II CATG 1 cut(s) 455
HinfI GANTC 8 cut(s) 83, 163, 257, 407, 413, 431, 899, 1280
HpaII CCGG 1 cut(s) 665
HphI GGTGA 3 cut(s) 800, 1076, 1117
Hpy166II GTNNAC 2 cut(s) 715, 1284
Hpy188III TCNNGA 3 cut(s) 526, 925, 968
Hpy8I GTNNAC 2 cut(s) 715, 1284
Hpy99I CGWCG 4 cut(s) 284, 287, 443, 1232
HpyAV CCTTC 8 cut(s) 868, 946, 1108, 1123, 1213, 1228, 1303, 1343
HpyCH4III ACNGT 2 cut(s) 707, 853
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 3 cut(s) 764, 1061, 1169
HpyF10VI GCNNNNNNNGC 3 cut(s) 349, 764, 1087
HpyF3I CTNAG 4 cut(s) 124, 165, 201, 642
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 1 cut(s) 455
Ksp22I TGATCA 1 cut(s) 862
Kzo9I GATC 8 cut(s) 862, 940, 964, 1017, 1066, 1291, 1318, 1334
LguI GCTCTTC 2 cut(s) 717, 1136
LmnI GCTCC 3 cut(s) 349, 357, 1132
Lsp1109I GCAGC 8 cut(s) 167, 170, 203, 206, 304, 748, 776, 805
MaeII ACGT 1 cut(s) 285
MalI GATC 8 cut(s) 864, 942, 966, 1019, 1068, 1293, 1320, 1336
MbiI CCGCTC 1 cut(s) 733
MboI GATC 8 cut(s) 862, 940, 964, 1017, 1066, 1291, 1318, 1334
MflI RGATCY 1 cut(s) 964
MhlI GDGCHC 1 cut(s) 354
MlyI GAGTC 5 cut(s) 92, 157, 251, 407, 1289
MmeI TCCRAC 3 cut(s) 379, 1155, 1284
MseI TTAA 6 cut(s) 137, 552, 636, 936, 1239, 1266
MspA1I CMGCKG 3 cut(s) 249, 761, 946
MspCI CTTAAG 1 cut(s) 1265
MspI CCGG 1 cut(s) 665
MspR9I CCNGG 3 cut(s) 665, 1052, 1087
Mva1269I GAATGC 3 cut(s) 820, 1063, 1081
MvaI CCWGG 2 cut(s) 1052, 1087
MwoI GCNNNNNNNGC 3 cut(s) 349, 764, 1087
NciI CCSGG 1 cut(s) 665
NdeII GATC 8 cut(s) 862, 940, 964, 1017, 1066, 1291, 1318, 1334
NlaIII CATG 1 cut(s) 455
NlaIV GGNNCC 3 cut(s) 565, 966, 1049
NmeAIII GCCGAG 1 cut(s) 793
PaqCI CACCTGC 1 cut(s) 1052
PceI AGGCCT 1 cut(s) 384
PciSI GCTCTTC 2 cut(s) 717, 1136
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PctI GAATGC 3 cut(s) 820, 1063, 1081
PfeI GAWTC 3 cut(s) 407, 431, 899
PflFI GACNNNGTC 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 1050
PkrI GCNGC 9 cut(s) 157, 160, 193, 196, 294, 760, 763, 766, 820
Ple19I CGATCG 1 cut(s) 1337
PleI GAGTC 5 cut(s) 91, 157, 251, 407, 1288
PpsI GAGTC 5 cut(s) 91, 157, 251, 407, 1288
PshBI ATTAAT 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 1050, 1085
PspGI CCWGG 2 cut(s) 1050, 1085
PspN4I GGNNCC 3 cut(s) 565, 966, 1049
PspPI GGNCC 2 cut(s) 52, 662
PstI CTGCAG 1 cut(s) 766
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 964
PsyI GACNNNGTC 1 cut(s) 446
PvuI CGATCG 1 cut(s) 1337
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 3 cut(s) 326, 623, 1043
RsaNI GTAC 3 cut(s) 325, 622, 1042
SacI GAGCTC 1 cut(s) 354
SapI GCTCTTC 2 cut(s) 717, 1136
SaqAI TTAA 6 cut(s) 137, 552, 636, 936, 1239, 1266
SatI GCNGC 9 cut(s) 156, 159, 192, 195, 293, 759, 762, 765, 819
Sau3AI GATC 8 cut(s) 862, 940, 964, 1017, 1066, 1291, 1318, 1334
Sau96I GGNCC 2 cut(s) 52, 662
SchI GAGTC 5 cut(s) 92, 157, 251, 407, 1289
ScrFI CCNGG 3 cut(s) 665, 1052, 1087
SduI GDGCHC 1 cut(s) 354
SfcI CTRYAG 1 cut(s) 762
SinI GGWCC 1 cut(s) 52
SmlI CTYRAG 4 cut(s) 10, 500, 923, 1265
SmoI CTYRAG 4 cut(s) 10, 500, 923, 1265
SseBI AGGCCT 1 cut(s) 384
SstI GAGCTC 1 cut(s) 354
StuI AGGCCT 1 cut(s) 384
StyD4I CCNGG 3 cut(s) 663, 1050, 1085
TaaI ACNGT 2 cut(s) 707, 853
TaiI ACGT 1 cut(s) 288
TaqI TCGA 3 cut(s) 243, 1020, 1333
TaqII GACCGA 1 cut(s) 40
TatI WGTACW 2 cut(s) 621, 1041
TauI GCSGC 1 cut(s) 761
TfiI GAWTC 3 cut(s) 407, 431, 899
Tru1I TTAA 6 cut(s) 137, 552, 636, 936, 1239, 1266
Tru9I TTAA 6 cut(s) 137, 552, 636, 936, 1239, 1266
TseI GCWGC 8 cut(s) 155, 158, 191, 194, 292, 761, 764, 818
TspDTI ATGAA 4 cut(s) 23, 321, 921, 1359
TspGWI ACGGA 1 cut(s) 288
Tth111I GACNNNGTC 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 1265
VpaK11BI GGWCC 1 cut(s) 52
VspI ATTAAT 1 cut(s) 137
XapI RAATTY 4 cut(s) 460, 482, 746, 1207
XmiI GTMKAC 1 cut(s) 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.