pycom05g02730

F-actin-capping protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010468
Physical Location & Seq
Forward (+)
3234002 .. 3236756
2755 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
ATGTTAAGATGTATTAAGTATTTGAGATGTATTCAACAATATAAAAAGCCTAAAAAAATTCAGTGTGTGAGAGAGAGATTTGGAGAGGGAAACTCGGGTCAAACGAAGCAAGGCGCCTCTTTTCTTTTTGCCTCGCTTCTCTGGAGTTTTATCAACCCGAACAAAAAACCAAACAAAAGGAAAAGAAAAGGAAAGAGGAAAAATGGAAGCGGCGATGGGACTGATGCGGCGGATGCCTCCCAAGCACACCGAGACGTCTCTCTCCGCCCTTCTCTCCCTTCTCCCCCAGCACTCCTCCGATCGATCCTCTCTCAAATCGATCAGCCTCTCCAGGTTTTGTGCGACATGGAATACGGGAAGGAGTTCATTCTGTGTGAATATAACAGAGATGCAGACTCTTACAGATCACCTTGGTCAAACAAATATCATCCGCCACTAGAAGACGGCAGCCTCCCATCTTTAGAGCTGAGGAAACTTGAAGTTGAAGCCAACGACATCTTTGCCATTTATCGTGACCAGTATTATGAAGGTGGCATTTCATCAGTTTATATGTGGGAGGATGATAACGAAGGTTTTGTAGGCTGCTTTTTAATAAAGAAAGATGGCTCCAAGACAGGGCAAGGTCGGCGAGGTTATCTGCAGGAGGGGGCATGGGATGCTATACATGTGATTGAGGTGGGTCCGGAGGAGGAAGGAACAGCTCATTACCGTTTAACAAGTACTGTAATGCTGTCTTTGACTACAGATAATGAATCGTCAGGCACATTCAGTTTGTCTGGATCAATTAGACGACAGATGAATATGCATCTGTCAACTGAAGAAGGCCATCTTTGTAACATGGGAAGAATGATTGAAGAAATGGAGAGCAAGCTGAGAAATTCACTGGATCAGGTTTATTTTGGGAAGACGAAAGAGATGGTTTGCACATTACGACCACCATCTGAAGTAGTGATGAGACTGCCTGACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F_actin_cap_B

Protein Analysis

324

Amino Acids

36.69

Weight (kDa)

8.03

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F_actin_cap_B PF01115 96 - 307 1.2e-86 F-actin capping protein, beta subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AccB1I GGYRCC 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 5 cut(s) 210, 227, 230, 265, 431
AclWI GGATC 3 cut(s) 298, 787, 894
AcsI RAATTY 2 cut(s) 57, 877
AcuI CTGAAG 2 cut(s) 837, 963
AcyI GRCGYC 2 cut(s) 114, 255
AfaI GTAC 1 cut(s) 721
AfiI CCNNNNNNNGG 1 cut(s) 615
AflIII ACRYGT 1 cut(s) 664
AgsI TTSAA 4 cut(s) 35, 479, 485, 854
AjnI CCWGG 1 cut(s) 330
AluBI AGCT 4 cut(s) 466, 701, 871, 969
AluI AGCT 4 cut(s) 466, 701, 871, 969
Alw26I GTCTC 3 cut(s) 246, 262, 949
AlwI GGATC 3 cut(s) 298, 787, 894
Ama87I CYCGRG 1 cut(s) 94
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 1 cut(s) 823
ApeKI GCWGC 2 cut(s) 447, 582
ApoI RAATTY 2 cut(s) 57, 877
Asp700I GAANNNNTTC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 680
AsuHPI GGTGA 1 cut(s) 399
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 1 cut(s) 680
BanI GGYRCC 1 cut(s) 113
BbsI GAAGAC 2 cut(s) 447, 911
BbvCI CCTCAGC 1 cut(s) 467
BbvI GCAGC 2 cut(s) 459, 569
BccI CCATC 6 cut(s) 209, 463, 596, 834, 910, 946
BceAI ACGGC 1 cut(s) 460
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 1 cut(s) 332
BcoDI GTCTC 3 cut(s) 246, 262, 949
BfaI CTAG 1 cut(s) 437
BfmI CTRYAG 2 cut(s) 638, 741
BfoI RGCGCY 1 cut(s) 117
BisI GCNGC 4 cut(s) 211, 228, 448, 583
BlsI GCNGC 4 cut(s) 212, 229, 449, 584
BmcAI AGTACT 1 cut(s) 721
Bme1390I CCNGG 1 cut(s) 332
Bme18I GGWCC 1 cut(s) 680
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 3 cut(s) 115, 607, 681
BmrFI CCNGG 1 cut(s) 332
BmsI GCATC 5 cut(s) 214, 223, 379, 646, 814
BpiI GAAGAC 2 cut(s) 447, 911
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpmI CTGGAG 2 cut(s) 163, 314
Bpu10I CCTNAGC 1 cut(s) 467
Bsa29I ATCGAT 2 cut(s) 302, 318
BsaHI GRCGYC 2 cut(s) 114, 255
BsaJI CCNNGG 1 cut(s) 410
BsaWI WCCGGW 1 cut(s) 682
Bsc4I CCNNNNNNNGG 1 cut(s) 615
Bse1I ACTGG 2 cut(s) 517, 888
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 1 cut(s) 332
BseCI ATCGAT 2 cut(s) 302, 318
BseDI CCNNGG 1 cut(s) 410
BseGI GGATG 4 cut(s) 238, 427, 565, 661
BseLI CCNNNNNNNGG 1 cut(s) 615
BseMII CTCAG 2 cut(s) 458, 863
BseNI ACTGG 2 cut(s) 517, 888
BseRI GAGGAG 2 cut(s) 284, 701
BseXI GCAGC 2 cut(s) 459, 569
BseYI CCCAGC 1 cut(s) 286
Bsh1285I CGRYCG 1 cut(s) 302
BshFI GGCC 1 cut(s) 825
BshNI GGYRCC 1 cut(s) 113
BshVI ATCGAT 2 cut(s) 302, 318
BsiEI CGRYCG 1 cut(s) 302
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 1 cut(s) 683
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 1 cut(s) 615
BsmAI GTCTC 3 cut(s) 246, 262, 949
BsmBI CGTCTC 2 cut(s) 246, 262
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 1 cut(s) 825
BsoBI CYCGRG 1 cut(s) 94
Bsp13I TCCGGA 1 cut(s) 682
Bsp143I GATC 6 cut(s) 299, 303, 319, 404, 779, 886
BspACI CCGC 5 cut(s) 210, 227, 230, 265, 431
BspANI GGCC 1 cut(s) 825
BspCNI CTCAG 2 cut(s) 459, 864
BspDI ATCGAT 2 cut(s) 302, 318
BspEI TCCGGA 1 cut(s) 682
BspLI GGNNCC 3 cut(s) 115, 607, 681
BspMAI CTGCAG 1 cut(s) 642
BspPI GGATC 3 cut(s) 298, 787, 894
BspT107I GGYRCC 1 cut(s) 113
BsrI ACTGG 2 cut(s) 517, 888
BssECI CCNNGG 1 cut(s) 410
BssMI GATC 6 cut(s) 299, 303, 319, 404, 779, 886
BssNI GRCGYC 2 cut(s) 114, 255
BssT1I CCWWGG 1 cut(s) 410
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 2 cut(s) 710, 724
BstACI GRCGYC 2 cut(s) 114, 255
BstAPI GCANNNNNTGC 1 cut(s) 656
BstC8I GCNNGC 1 cut(s) 869
BstDEI CTNAG 2 cut(s) 467, 872
BstF5I GGATG 4 cut(s) 238, 427, 565, 661
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 6 cut(s) 302, 306, 322, 407, 782, 889
BstMAI GTCTC 3 cut(s) 246, 262, 949
BstMBI GATC 6 cut(s) 299, 303, 319, 404, 779, 886
BstMCI CGRYCG 1 cut(s) 302
BstMWI GCNNNNNNNGC 4 cut(s) 233, 242, 625, 656
BstNI CCWGG 1 cut(s) 332
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 1 cut(s) 330
BstSFI CTRYAG 2 cut(s) 638, 741
BstV1I GCAGC 2 cut(s) 459, 569
BstV2I GAAGAC 2 cut(s) 447, 911
Bsu15I ATCGAT 2 cut(s) 302, 318
BsuRI GGCC 1 cut(s) 825
BsuTUI ATCGAT 2 cut(s) 302, 318
BtgZI GCGATG 1 cut(s) 228
BtsCI GGATG 4 cut(s) 238, 427, 565, 661
BtsIMutI CAGTG 2 cut(s) 68, 881
Cac8I GCNNGC 1 cut(s) 869
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 680
ClaI ATCGAT 2 cut(s) 302, 318
Csp6I GTAC 1 cut(s) 720
CviAII CATG 4 cut(s) 346, 651, 665, 838
CviQI GTAC 1 cut(s) 720
DdeI CTNAG 2 cut(s) 467, 872
DinI GGCGCC 1 cut(s) 115
DpnI GATC 6 cut(s) 301, 305, 321, 406, 781, 888
DpnII GATC 6 cut(s) 299, 303, 319, 404, 779, 886
EciI GGCGGA 3 cut(s) 245, 254, 420
Eco130I CCWWGG 1 cut(s) 410
Eco47I GGWCC 1 cut(s) 680
Eco57I CTGAAG 2 cut(s) 837, 963
Eco88I CYCGRG 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 330
EcoT14I CCWWGG 1 cut(s) 410
EcoT22I ATGCAT 1 cut(s) 807
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 410
Esp3I CGTCTC 2 cut(s) 246, 262
FaeI CATG 4 cut(s) 349, 654, 668, 841
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FaqI GGGAC 1 cut(s) 232
FatI CATG 4 cut(s) 345, 650, 664, 837
Fnu4HI GCNGC 4 cut(s) 211, 228, 448, 583
FokI GGATG 4 cut(s) 245, 414, 572, 668
Fsp4HI GCNGC 4 cut(s) 211, 228, 448, 583
FspBI CTAG 1 cut(s) 437
GlaI GCGC 1 cut(s) 115
GluI GCNGC 4 cut(s) 211, 228, 448, 583
GsaI CCCAGC 1 cut(s) 290
GsuI CTGGAG 2 cut(s) 163, 314
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 1 cut(s) 825
HapII CCGG 1 cut(s) 683
HhaI GCGC 1 cut(s) 116
Hin1I GRCGYC 2 cut(s) 114, 255
Hin1II CATG 4 cut(s) 349, 654, 668, 841
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HinfI GANTC 2 cut(s) 395, 752
HpaII CCGG 1 cut(s) 683
HphI GGTGA 1 cut(s) 399
Hpy166II GTNNAC 1 cut(s) 813
Hpy188I TCNGA 2 cut(s) 299, 943
Hpy188III TCNNGA 4 cut(s) 142, 512, 683, 777
Hpy8I GTNNAC 1 cut(s) 813
HpyAV CCTTC 7 cut(s) 279, 288, 352, 521, 563, 686, 815
HpyCH4III ACNGT 2 cut(s) 710, 724
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 4 cut(s) 392, 640, 805, 924
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 242, 625, 656
HpyF3I CTNAG 2 cut(s) 467, 872
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 2 cut(s) 114, 255
Hsp92II CATG 4 cut(s) 349, 654, 668, 841
HspAI GCGC 1 cut(s) 114
KasI GGCGCC 1 cut(s) 113
Kpn2I TCCGGA 1 cut(s) 682
Kzo9I GATC 6 cut(s) 299, 303, 319, 404, 779, 886
LmnI GCTCC 1 cut(s) 611
Lsp1109I GCAGC 2 cut(s) 459, 569
LweI GCATC 5 cut(s) 214, 223, 379, 646, 814
MaeI CTAG 1 cut(s) 437
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 512, 833
MalI GATC 6 cut(s) 301, 305, 321, 406, 781, 888
MboI GATC 6 cut(s) 299, 303, 319, 404, 779, 886
MboII GAAGA 5 cut(s) 452, 830, 855, 866, 916
MluCI AATT 3 cut(s) 57, 783, 877
Mly113I GGCGCC 1 cut(s) 114
MlyI GAGTC 1 cut(s) 389
Mph1103I ATGCAT 1 cut(s) 807
MroI TCCGGA 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 362
MseI TTAA 4 cut(s) 5, 15, 590, 713
MspA1I CMGCKG 1 cut(s) 969
MspI CCGG 1 cut(s) 683
MspR9I CCNGG 1 cut(s) 332
MvaI CCWGG 1 cut(s) 332
MwoI GCNNNNNNNGC 4 cut(s) 233, 242, 625, 656
NarI GGCGCC 1 cut(s) 114
NdeII GATC 6 cut(s) 299, 303, 319, 404, 779, 886
NlaIII CATG 4 cut(s) 349, 654, 668, 841
NlaIV GGNNCC 3 cut(s) 115, 607, 681
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 807
NspI RCATGY 1 cut(s) 668
PciI ACATGT 1 cut(s) 664
PcsI WCGNNNNNNNCGW 1 cut(s) 101
PdmI GAANNNNTTC 1 cut(s) 362
PfeI GAWTC 1 cut(s) 752
PkrI GCNGC 4 cut(s) 212, 229, 449, 584
Ple19I CGATCG 1 cut(s) 302
PleI GAGTC 1 cut(s) 389
PluTI GGCGCC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 389
PscI ACATGT 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 330
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 3 cut(s) 115, 607, 681
PspPI GGNCC 1 cut(s) 680
PstI CTGCAG 1 cut(s) 642
PvuI CGATCG 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 969
RsaI GTAC 1 cut(s) 721
RsaNI GTAC 1 cut(s) 720
SaqAI TTAA 4 cut(s) 5, 15, 590, 713
SatI GCNGC 4 cut(s) 211, 228, 448, 583
Sau3AI GATC 6 cut(s) 299, 303, 319, 404, 779, 886
Sau96I GGNCC 1 cut(s) 680
ScaI AGTACT 1 cut(s) 721
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 332
SfaNI GCATC 5 cut(s) 214, 223, 379, 646, 814
SfcI CTRYAG 2 cut(s) 638, 741
SfoI GGCGCC 1 cut(s) 115
SinI GGWCC 1 cut(s) 680
Sse9I AATT 3 cut(s) 57, 783, 877
SsiI CCGC 5 cut(s) 210, 227, 230, 265, 431
SspDI GGCGCC 1 cut(s) 113
SspMI CTAG 1 cut(s) 437
StyD4I CCNGG 1 cut(s) 330
StyI CCWWGG 1 cut(s) 410
TaaI ACNGT 2 cut(s) 710, 724
TaiI ACGT 1 cut(s) 258
TaqI TCGA 2 cut(s) 302, 318
TasI AATT 3 cut(s) 57, 783, 877
TatI WGTACW 1 cut(s) 719
TauI GCSGC 2 cut(s) 213, 230
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 4 cut(s) 5, 15, 590, 713
Tru9I TTAA 4 cut(s) 5, 15, 590, 713
TscAI CASTG 2 cut(s) 68, 888
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 2 cut(s) 447, 582
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 5 cut(s) 355, 528, 540, 765, 812
TspRI CASTG 2 cut(s) 68, 888
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 2 cut(s) 57, 877
XceI RCATGY 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 362
XspI CTAG 1 cut(s) 437
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 1 cut(s) 721
Zsp2I ATGCAT 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.