pycom05g04590

30S ribosomal protein S31

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015448
Physical Location & Seq
Forward (+)
5425794 .. 5425988
195 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04590.1

Sequence Viewer

Length: 195 bp
ATGGCGGAGCAGCGCGCCATCTTCACAACCTCCTCTTCTTCCTCCCCGGCAGCAGCAGCAATTGCAAAAGCAAATGTGCTTGGGGCGCCGATGCTGTGCGGGCGAGGAGACAAGAAGACGAAGAAGGGGAAGCGATTCAAAGGGTCGTTCGGAAACGCTCGGCCGAAGAAGGAGAAGATGGATCGAGCGCATTAA

Protein Analysis

65

Amino Acids

6.79

Weight (kDa)

10.83

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPS31 PF17067 31 - 61 5e-08 Ribosomal protein S31e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015448)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21290
fragaria_vesca FvH4_2g10470
malus_domestica MD05G1055600.v1.1 MD10G1062900.v1.1
prunus_persica Prupe.8G083700_v2.0.a1
pyrus_communis pycom05g04590 pycom10g04860
rosa_chinensis RchiOBHm_Chr6g0267951
rosa_multiflora Rmu_sc0000434.1_g000018
rosa_roxburghii Rroxscaffold_7G00200300
rosa_rugosa Rorug06G0035700
rosa_samantha Rh6BG159800 Rh6CG157000 Rh6DG145500
rosa_wichuraiana Rw0G019630 Rw6G013720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 85
AccII CGCG 1 cut(s) 15
AciI CCGC 2 cut(s) 5, 99
AclWI GGATC 1 cut(s) 189
AcoI YGGCCR 1 cut(s) 161
AcyI GRCGYC 1 cut(s) 86
AgsI TTSAA 1 cut(s) 139
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 1 cut(s) 189
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 4 cut(s) 10, 50, 53, 56
Asp700I GAANNNNTTC 1 cut(s) 134
AspLEI GCGC 4 cut(s) 15, 17, 88, 190
AsuC2I CCSGG 1 cut(s) 47
BanI GGYRCC 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 122
BbvI GCAGC 4 cut(s) 22, 62, 65, 68
BccI CCATC 2 cut(s) 26, 172
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 102
BfoI RGCGCY 1 cut(s) 89
BisI GCNGC 4 cut(s) 11, 51, 54, 57
BlsI GCNGC 4 cut(s) 12, 52, 55, 58
Bme1390I CCNGG 1 cut(s) 47
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 45
BsePI GCGCGC 1 cut(s) 13
BseRI GAGGAG 2 cut(s) 22, 120
BseX3I CGGCCG 1 cut(s) 161
BseXI GCAGC 4 cut(s) 22, 62, 65, 68
Bsh1236I CGCG 1 cut(s) 15
Bsh1285I CGRYCG 1 cut(s) 164
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 85
BsiEI CGRYCG 1 cut(s) 164
BsiSI CCGG 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 102
BsnI GGCC 1 cut(s) 163
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 2 cut(s) 5, 99
BspANI GGCC 1 cut(s) 163
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 87
BspPI GGATC 1 cut(s) 189
BspT107I GGYRCC 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 45
BssHII GCGCGC 1 cut(s) 13
BssMI GATC 1 cut(s) 181
BssNI GRCGYC 1 cut(s) 86
Bst6I CTCTTC 1 cut(s) 40
BstACI GRCGYC 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 62
BstC8I GCNNGC 2 cut(s) 15, 101
BstFNI CGCG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 4 cut(s) 15, 17, 88, 190
BstKTI GATC 1 cut(s) 184
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 1 cut(s) 181
BstMCI CGRYCG 1 cut(s) 164
BstMWI GCNNNNNNNGC 4 cut(s) 56, 62, 85, 100
BstSCI CCNGG 1 cut(s) 45
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 4 cut(s) 22, 62, 65, 68
BstV2I GAAGAC 1 cut(s) 122
BstZI CGGCCG 1 cut(s) 161
BsuRI GGCC 1 cut(s) 163
Cac8I GCNNGC 2 cut(s) 15, 101
CfoI GCGC 4 cut(s) 15, 17, 88, 190
CviJI RGCY 1 cut(s) 163
CviKI_1 RGCY 1 cut(s) 163
DinI GGCGCC 1 cut(s) 87
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
EaeI YGGCCR 1 cut(s) 161
EagI CGGCCG 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 161
Eco52I CGGCCG 1 cut(s) 161
EgeI GGCGCC 1 cut(s) 87
EheI GGCGCC 1 cut(s) 87
FauI CCCGC 1 cut(s) 92
Fnu4HI GCNGC 4 cut(s) 11, 51, 54, 57
Fsp4HI GCNGC 4 cut(s) 11, 51, 54, 57
GlaI GCGC 4 cut(s) 14, 16, 87, 189
GluI GCNGC 4 cut(s) 11, 51, 54, 57
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 1 cut(s) 163
HapII CCGG 1 cut(s) 47
HhaI GCGC 4 cut(s) 15, 17, 88, 190
Hin1I GRCGYC 1 cut(s) 86
Hin6I GCGC 4 cut(s) 13, 15, 86, 188
HinP1I GCGC 4 cut(s) 13, 15, 86, 188
HinfI GANTC 1 cut(s) 135
HpaII CCGG 1 cut(s) 47
Hpy188I TCNGA 1 cut(s) 152
HpyAV CCTTC 2 cut(s) 118, 163
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 62, 85, 100
Hsp92I GRCGYC 1 cut(s) 86
HspAI GCGC 4 cut(s) 13, 15, 86, 188
KasI GGCGCC 1 cut(s) 85
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 1 cut(s) 7
LpnPI CCDG 1 cut(s) 60
Lsp1109I GCAGC 4 cut(s) 22, 62, 65, 68
LweI GCATC 1 cut(s) 81
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 7 cut(s) 13, 27, 30, 127, 133, 178, 187
MfeI CAATTG 1 cut(s) 60
MluCI AATT 1 cut(s) 60
Mly113I GGCGCC 1 cut(s) 86
MnlI CCTC 4 cut(s) 40, 43, 52, 98
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 1 cut(s) 193
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 47
MunI CAATTG 1 cut(s) 60
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 4 cut(s) 56, 62, 85, 100
NarI GGCGCC 1 cut(s) 86
NciI CCSGG 1 cut(s) 47
NdeII GATC 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 87
NmeAIII GCCGAG 1 cut(s) 139
PauI GCGCGC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 135
PkrI GCNGC 4 cut(s) 12, 52, 55, 58
PluTI GGCGCC 1 cut(s) 89
PspN4I GGNNCC 1 cut(s) 87
PteI GCGCGC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 193
SatI GCNGC 4 cut(s) 11, 51, 54, 57
Sau3AI GATC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 47
SetI ASST 1 cut(s) 32
SfaNI GCATC 1 cut(s) 81
SfoI GGCGCC 1 cut(s) 87
SgeI CNNG 8 cut(s) 26, 58, 59, 92, 112, 116, 124, 171
Sse9I AATT 1 cut(s) 60
SsiI CCGC 2 cut(s) 5, 99
SspDI GGCGCC 1 cut(s) 85
StyD4I CCNGG 1 cut(s) 45
TaqI TCGA 1 cut(s) 184
TasI AATT 1 cut(s) 60
TfiI GAWTC 1 cut(s) 135
Tru1I TTAA 1 cut(s) 193
Tru9I TTAA 1 cut(s) 193
TseI GCWGC 4 cut(s) 10, 50, 53, 56
XmnI GAANNNNTTC 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.