Rw6G013720

30S ribosomal protein S31

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015448
Physical Location & Seq
Reverse (-)
25346420 .. 25346704
285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013720.1

Sequence Viewer

Length: 285 bp
ATGGCGATGGTGCAGTGGTGCGGCGCAGCGGCAAGGCGAGTGTTGGTGAGGGAGCACCGCGCCCTCTTCACGACCTCGTCATCGTCGACGTCATCAGCTCAAAGCAGTGGAATCGGGGCTCCGATGCTGTGCGGGCGAGGGGACAAGAAGACGAAGAAGGGGAAGCGATTCAAAGGGTCGTACGGGAACGCTAGGCCGAAGAAGGAGAAGACGATCGAGCGCATCAAGGACAAGGTTGAGGTTCCCAGGTCCACTCCTTGGCCTCTCCCTTTCAAGCTCATTTGA

Protein Analysis

94

Amino Acids

10.39

Weight (kDa)

10.86

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPS31 PF17067 27 - 86 1.6e-12 Ribosomal protein S31e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015448)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21290
fragaria_vesca FvH4_2g10470
malus_domestica MD05G1055600.v1.1 MD10G1062900.v1.1
prunus_persica Prupe.8G083700_v2.0.a1
pyrus_communis pycom05g04590 pycom10g04860
rosa_chinensis RchiOBHm_Chr6g0267951
rosa_multiflora Rmu_sc0000434.1_g000018
rosa_roxburghii Rroxscaffold_7G00200300
rosa_rugosa Rorug06G0035700
rosa_samantha Rh6BG159800 Rh6CG157000 Rh6DG145500
rosa_wichuraiana Rw0G019630 Rw6G013720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 258
AccI GTMKAC 1 cut(s) 86
AccII CGCG 1 cut(s) 60
AciI CCGC 4 cut(s) 21, 29, 58, 132
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 2 cut(s) 172, 274
AjnI CCWGG 1 cut(s) 245
AluBI AGCT 2 cut(s) 98, 277
AluI AGCT 2 cut(s) 98, 277
Alw21I GWGCWC 1 cut(s) 57
AoxI GGCC 2 cut(s) 194, 260
ApeKI GCWGC 1 cut(s) 26
Asp700I GAANNNNTTC 1 cut(s) 167
AspLEI GCGC 3 cut(s) 26, 62, 222
AspS9I GGNCC 1 cut(s) 249
AsuHPI GGTGA 1 cut(s) 58
AvaII GGWCC 1 cut(s) 249
BanII GRGCYC 1 cut(s) 121
BbsI GAAGAC 2 cut(s) 155, 215
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 1 cut(s) 247
BfaI CTAG 1 cut(s) 192
BisI GCNGC 3 cut(s) 22, 27, 30
BlsI GCNGC 3 cut(s) 23, 28, 31
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 249
BmiI GGNNCC 2 cut(s) 120, 243
BmrFI CCNGG 1 cut(s) 247
BmsI GCATC 2 cut(s) 114, 231
BpiI GAAGAC 2 cut(s) 155, 215
BsaHI GRCGYC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 245, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 258
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 2 cut(s) 245, 257
BseLI CCNNNNNNNGG 1 cut(s) 258
BseXI GCAGC 1 cut(s) 38
BsgI GTGCAG 1 cut(s) 32
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 2 cut(s) 196, 262
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 57
BsiWI CGTACG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 2 cut(s) 196, 262
Bsp1286I GDGCHC 2 cut(s) 57, 121
Bsp143I GATC 1 cut(s) 213
BspACI CCGC 4 cut(s) 21, 29, 58, 132
BspANI GGCC 2 cut(s) 196, 262
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 120, 243
BssECI CCNNGG 2 cut(s) 245, 257
BssMI GATC 1 cut(s) 213
BssNI GRCGYC 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 134
BstFNI CGCG 1 cut(s) 60
BstHHI GCGC 3 cut(s) 26, 62, 222
BstKTI GATC 1 cut(s) 216
BstMBI GATC 1 cut(s) 213
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 245
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 38
BstV2I GAAGAC 2 cut(s) 155, 215
BsuRI GGCC 2 cut(s) 196, 262
BtgZI GCGATG 1 cut(s) 20
BtsI GCAGTG 2 cut(s) 20, 112
BtsIMutI CAGTG 2 cut(s) 20, 112
Cac8I GCNNGC 1 cut(s) 134
CfoI GCGC 3 cut(s) 26, 62, 222
Cfr13I GGNCC 1 cut(s) 249
Csp6I GTAC 1 cut(s) 181
CviJI RGCY 5 cut(s) 98, 119, 196, 262, 277
CviKI_1 RGCY 5 cut(s) 98, 119, 196, 262, 277
CviQI GTAC 1 cut(s) 181
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 257
Eco24I GRGCYC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 257
EcoT38I GRGCYC 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 257
FaqI GGGAC 1 cut(s) 155
FauI CCCGC 1 cut(s) 125
FblI GTMKAC 1 cut(s) 86
Fnu4HI GCNGC 3 cut(s) 22, 27, 30
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 3 cut(s) 22, 27, 30
FspBI CTAG 1 cut(s) 192
GlaI GCGC 3 cut(s) 25, 61, 221
GluI GCNGC 3 cut(s) 22, 27, 30
HaeIII GGCC 2 cut(s) 196, 262
HhaI GCGC 3 cut(s) 26, 62, 222
Hin1I GRCGYC 1 cut(s) 89
Hin6I GCGC 3 cut(s) 24, 60, 220
HinP1I GCGC 3 cut(s) 24, 60, 220
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 2 cut(s) 111, 168
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 2 cut(s) 87, 252
Hpy188I TCNGA 1 cut(s) 123
Hpy188III TCNNGA 1 cut(s) 70
Hpy8I GTNNAC 2 cut(s) 87, 252
Hpy99I CGWCG 2 cut(s) 88, 91
HpyAV CCTTC 2 cut(s) 151, 196
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 1 cut(s) 13
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpySE526I ACGT 1 cut(s) 89
Hsp92I GRCGYC 1 cut(s) 89
HspAI GCGC 3 cut(s) 24, 60, 220
Kzo9I GATC 1 cut(s) 213
LmnI GCTCC 2 cut(s) 52, 124
LpnPI CCDG 2 cut(s) 232, 259
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 2 cut(s) 114, 231
MaeI CTAG 1 cut(s) 192
MaeII ACGT 1 cut(s) 89
MalI GATC 1 cut(s) 215
MboI GATC 1 cut(s) 213
MboII GAAGA 5 cut(s) 58, 160, 166, 211, 220
MhlI GDGCHC 2 cut(s) 57, 121
MnlI CCTC 6 cut(s) 42, 74, 85, 131, 232, 273
MroXI GAANNNNTTC 1 cut(s) 167
MspA1I CMGCKG 1 cut(s) 29
MspR9I CCNGG 1 cut(s) 247
MvaI CCWGG 1 cut(s) 247
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 1 cut(s) 133
NdeII GATC 1 cut(s) 213
NlaIV GGNNCC 2 cut(s) 120, 243
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 111, 168
Pfl23II CGTACG 1 cut(s) 180
PflFI GACNNNGTC 1 cut(s) 76
PflMI CCANNNNNTGG 1 cut(s) 258
PkrI GCNGC 3 cut(s) 23, 28, 31
Ple19I CGATCG 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 245
PspGI CCWGG 1 cut(s) 245
PspLI CGTACG 1 cut(s) 180
PspN4I GGNNCC 2 cut(s) 120, 243
PspPI GGNCC 1 cut(s) 249
PsyI GACNNNGTC 1 cut(s) 76
PvuI CGATCG 1 cut(s) 216
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SalI GTCGAC 1 cut(s) 85
SatI GCNGC 3 cut(s) 22, 27, 30
Sau3AI GATC 1 cut(s) 213
Sau96I GGNCC 1 cut(s) 249
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 2 cut(s) 57, 121
SetI ASST 7 cut(s) 77, 92, 100, 237, 243, 251, 279
SfaNI GCATC 2 cut(s) 114, 231
SgrDI CGTCGACG 1 cut(s) 85
SinI GGWCC 1 cut(s) 249
SsiI CCGC 4 cut(s) 21, 29, 58, 132
SspMI CTAG 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 257
TaiI ACGT 1 cut(s) 92
TaqI TCGA 2 cut(s) 86, 216
TauI GCSGC 2 cut(s) 24, 32
TfiI GAWTC 2 cut(s) 111, 168
TscAI CASTG 2 cut(s) 20, 112
TseI GCWGC 1 cut(s) 26
TspRI CASTG 2 cut(s) 20, 112
Tth111I GACNNNGTC 1 cut(s) 76
Van91I CCANNNNNTGG 1 cut(s) 258
VpaK11BI GGWCC 1 cut(s) 249
XmiI GTMKAC 1 cut(s) 86
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 1 cut(s) 192
ZraI GACGTC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.