Rh6CG157000

30S ribosomal protein S31

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0015448
Physical Location & Seq
Forward (+)
20375365 .. 20376317
953 bp
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UTR
Exon/CDS
Intron
Rh6CG157000.1

Sequence Viewer

Length: 288 bp
ATGGCGATGGTGCAGTGGTGCGGCGCAGCGGCGAGACGAGTGTTGGTGAGGGAGCACCGTGCCCTCTTCACGACCTCGTCATCGCCGACGTCATCAGCTCAAGGCAGTGGAATCGGGGCTCCGATGCTGTGCGGGCGAGGGGACAAGAAGACGAAGAAGGGGAAGCGGTTCAAAGGTTCGTACGGGAACGCTAGGCCGAAGAAGGAGAAGATGATCGAGCGCATCAAGGACAAGGTTGAGGTTCCCAGATGCATGCCTCACCCCAATGAATTGACCAAGCTACCATGA

Protein Analysis

95

Amino Acids

10.48

Weight (kDa)

10.49

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPS31 PF17067 32 - 86 6.7e-11 Ribosomal protein S31e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015448)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21290
fragaria_vesca FvH4_2g10470
malus_domestica MD05G1055600.v1.1 MD10G1062900.v1.1
prunus_persica Prupe.8G083700_v2.0.a1
pyrus_communis pycom05g04590 pycom10g04860
rosa_chinensis RchiOBHm_Chr6g0267951
rosa_multiflora Rmu_sc0000434.1_g000018
rosa_roxburghii Rroxscaffold_7G00200300
rosa_rugosa Rorug06G0035700
rosa_samantha Rh6BG159800 Rh6CG157000 Rh6DG145500
rosa_wichuraiana Rw0G019630 Rw6G013720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 92
AciI CCGC 4 cut(s) 21, 29, 132, 166
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 1 cut(s) 182
AgsI TTSAA 1 cut(s) 172
AluBI AGCT 2 cut(s) 98, 280
AluI AGCT 2 cut(s) 98, 280
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 1 cut(s) 28
AoxI GGCC 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 26
Asp700I GAANNNNTTC 1 cut(s) 167
AspLEI GCGC 2 cut(s) 26, 222
AsuHPI GGTGA 2 cut(s) 58, 251
BaeGI GKGCMC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 1 cut(s) 192
BisI GCNGC 3 cut(s) 22, 27, 30
BlsI GCNGC 3 cut(s) 23, 28, 31
BmiI GGNNCC 2 cut(s) 120, 243
BmsI GCATC 3 cut(s) 114, 231, 239
BpiI GAAGAC 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 64
BseXI GCAGC 1 cut(s) 38
BsgI GTGCAG 1 cut(s) 32
BshFI GGCC 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 57
BsiWI CGTACG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 28
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 1 cut(s) 196
Bsp1286I GDGCHC 3 cut(s) 57, 64, 121
Bsp143I GATC 1 cut(s) 213
BspACI CCGC 4 cut(s) 21, 29, 132, 166
BspANI GGCC 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 120, 243
BssMI GATC 1 cut(s) 213
BssNI GRCGYC 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 89
BstC8I GCNNGC 2 cut(s) 134, 254
BstHHI GCGC 2 cut(s) 26, 222
BstKTI GATC 1 cut(s) 216
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNSI RCATGY 1 cut(s) 256
BstSLI GKGCMC 1 cut(s) 64
BstV1I GCAGC 1 cut(s) 38
BstV2I GAAGAC 1 cut(s) 155
BsuRI GGCC 1 cut(s) 196
BtgZI GCGATG 2 cut(s) 20, 66
BtsI GCAGTG 2 cut(s) 20, 112
BtsIMutI CAGTG 2 cut(s) 20, 112
Cac8I GCNNGC 2 cut(s) 134, 254
CfoI GCGC 2 cut(s) 26, 222
Csp6I GTAC 1 cut(s) 181
CviAII CATG 2 cut(s) 253, 285
CviJI RGCY 4 cut(s) 98, 119, 196, 280
CviKI_1 RGCY 4 cut(s) 98, 119, 196, 280
CviQI GTAC 1 cut(s) 181
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 254
EcoT38I GRGCYC 1 cut(s) 121
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 2 cut(s) 256, 288
FaiI YATR 2 cut(s) 254, 286
FaqI GGGAC 1 cut(s) 155
FatI CATG 2 cut(s) 252, 284
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 3 cut(s) 22, 27, 30
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 3 cut(s) 22, 27, 30
FspBI CTAG 1 cut(s) 192
GlaI GCGC 2 cut(s) 25, 221
GluI GCNGC 3 cut(s) 22, 27, 30
HaeIII GGCC 1 cut(s) 196
HhaI GCGC 2 cut(s) 26, 222
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 2 cut(s) 256, 288
Hin6I GCGC 2 cut(s) 24, 220
HinP1I GCGC 2 cut(s) 24, 220
HinfI GANTC 1 cut(s) 111
HphI GGTGA 2 cut(s) 58, 251
Hpy188I TCNGA 1 cut(s) 123
Hpy188III TCNNGA 1 cut(s) 70
Hpy99I CGWCG 1 cut(s) 91
HpyAV CCTTC 2 cut(s) 151, 196
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 2 cut(s) 13, 252
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpySE526I ACGT 1 cut(s) 89
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 2 cut(s) 256, 288
HspAI GCGC 2 cut(s) 24, 220
Kzo9I GATC 1 cut(s) 213
LmnI GCTCC 2 cut(s) 52, 124
LpnPI CCDG 1 cut(s) 259
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 3 cut(s) 114, 231, 239
MaeI CTAG 1 cut(s) 192
MaeII ACGT 1 cut(s) 89
MalI GATC 1 cut(s) 215
MboI GATC 1 cut(s) 213
MboII GAAGA 5 cut(s) 58, 160, 166, 211, 220
MhlI GDGCHC 3 cut(s) 57, 64, 121
MluCI AATT 1 cut(s) 269
MnlI CCTC 6 cut(s) 42, 74, 85, 131, 232, 267
Mph1103I ATGCAT 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 167
MslI CAYNNNNRTG 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 133
NdeII GATC 1 cut(s) 213
NlaIII CATG 2 cut(s) 256, 288
NlaIV GGNNCC 2 cut(s) 120, 243
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 1 cut(s) 256
PaeI GCATGC 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 1 cut(s) 111
Pfl23II CGTACG 1 cut(s) 180
PflFI GACNNNGTC 1 cut(s) 76
PkrI GCNGC 3 cut(s) 23, 28, 31
PspLI CGTACG 1 cut(s) 180
PspN4I GGNNCC 2 cut(s) 120, 243
PsyI GACNNNGTC 1 cut(s) 76
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 264
SatI GCNGC 3 cut(s) 22, 27, 30
Sau3AI GATC 1 cut(s) 213
SduI GDGCHC 3 cut(s) 57, 64, 121
SetI ASST 7 cut(s) 77, 92, 100, 178, 237, 243, 282
SfaNI GCATC 3 cut(s) 114, 231, 239
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
SphI GCATGC 1 cut(s) 256
Sse9I AATT 1 cut(s) 269
SsiI CCGC 4 cut(s) 21, 29, 132, 166
SspMI CTAG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 92
TaqI TCGA 1 cut(s) 216
TasI AATT 1 cut(s) 269
TauI GCSGC 2 cut(s) 24, 32
TfiI GAWTC 1 cut(s) 111
TscAI CASTG 2 cut(s) 20, 112
TseI GCWGC 1 cut(s) 26
TspDTI ATGAA 1 cut(s) 282
TspRI CASTG 2 cut(s) 20, 112
Tth111I GACNNNGTC 1 cut(s) 76
XceI RCATGY 1 cut(s) 256
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 1 cut(s) 192
ZraI GACGTC 1 cut(s) 90
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.