Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0010386

Physical Location & Seq

Genomic Coordinates Forward (+)

10226336 .. 10233595

Gene Structure 7260 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g07260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5160 bp

ATGTTTCCGTGGAACATCGCGAAGTCGGCGGAGGCGATGTTCTCGCGGTGGGCCGTGAAGAGAGTATGCAAGTTCCTGTTGAAGAAGAAGTTGGGGCAGTTCATATTGGGTGACATCGACGCCGACCAGCTCGATGTCCAGCTCTCCGAGGGCACCATTCAGCTCAACGACCTCGCGCTTAACGTCGATTTTCTTAATCAGAATTTTGGTGCAGCAGCATCGGTGATAATAAAAGAAGGATCTATTGGCTCCCTATTAGTTAGAATGCCTTGGAAGGGCAAAGGTTGTGAGGTTGAAGTGGATGAACTGGAGCTGGTGTTAAATCTGTGCGCAGAGAATAACACACCAGATAGTGCCGAAAGTTGCAACCTGGATAAGGATGCCAACCCAGGGACGTTAGATGGTGACATGGCAGACGATGCTGCAAAATCTGCTTCCAGGGATGTGCATGAAGGTGTTAAAACAATTGCAAAGAAGGTAAAGTGGTTTCTCACGAGCTTCCACGTAACGATAAAAAGGTTGATTGTTGCATTTGATCCTTGTTTAGAGAAGAATGGAAAGACATTAGGGTGTTCCTCAACCTTAGTGCTTCGAATTTTAGAAACAGAATGTGGAACGTGTGTTTCTGAAGATGATAGTCAAAATTCTGATTCAAGATTTGACAACTTTCTTGGGATTAGTCAATTGACCAACTTTGTCAAGTTTCAAGGAGCATCGCTTGAGCTGCTTCAAATGAATGATGCTGACAATCAAACATGCAGTCCATGCCAGTCAGAAAGAACATTTGGCGAATTTTTTCCAGCGTGCCGTCCACCGGGTGCCACCACTCCAATATTGGTTGGGAAAAGAGGGGGATTTTCAGGGAATATAAAATTAAGCATACCTTGGAAGAATGGGTCTTTAGACATTCGTAAAGTAGATGCAGATGTTTCTATTGAACCTTTAGAATTAAGATTTCAGCCCAGCACAACTAAATGGAGTTTACTTGCTTGGGAAAAATACAAAAATCTGGTGAAAGACAAGAGCAATCATAAGCCAGCGGATTCTGTTTATCTTGATTCAACATCTCACTGTATTTCACCAAGGTCTGTTTGTTCGACAACAGATAAGGTGAGGCCAAGTTGTGGTAGTTTTCCGACTGAATCATCTTCTTTTACCCTGCAAGAATCAGTGGCTGAACCTCTGCTGCCTGGGTCACATCTTATATCAGATTGGGTACCCTTTTTCATCCATCAAAATAAAAAAGATGGTATTGAAGAACTTGATTTTGGTGCAAGTGTGGATCAGTTTTTTGAATGTTTTGATGGAATGAGAAGTTCCCAATCAGCATTAGGTAACAGTGGGATGTGGAACTGGACGTGTTCTGTTTTCACTGCTATCACAGCTGCATCGAGCCTTGCTTCCGGATCTTTGCATGTTCCACCTGAGCAGCAGCATGTTGAAACAAATCTTAAAGCAGCTTTAGCTGGGATTTCTGTTGTCTTTTCATTTCAAGATGAAAAGCAGACGCATTTTTGTGATAATAAGGTGGTTGATTCAGATGTTCTGTACCTAGGTGCAGAATGCCGAGACATCCTTCTTGTTACGCAGGTATGCCCTCAAGAGATAAGGTTCAAAGGAACAATGGAGTATATCGAGGTTGCTAATTACTCAAGTTACAAAGAAGATACCTCTGAGTTTGGCTTTCAAAGGTCTAATACCAATATTAACAGCCATACTCTTTTGGTTCAACATCTTCAAGCTGATGTTCAAAGTGTTCTTCCACTGCTTCCCTCATCTTTTGAAGATACAGTTGTAGAGGTTCCATTTGGGTATAAGGATGTAGTCAGAACTACTTTACTTAAAACTTCAGGTGTTACTCACTGTCAGTTTACTGTGAGTTCTAGTACTTCAAATGGGAGTTTATGCGGGACATCATTTTCACTGAAACTGCCACATTTTGTTTTTTGGGTGGACTTCTCTTTGCTAAATATGCTATTAGAACTGTCTAATGAAGTGGGAAAGCCTGTCAAAGCGAACGGTAATCAGACTGAGTTTTCTTCTGAGTCCTCTAATAAGAAGCTTGGATCATCTCATGGGGATCTTAGAAGATCTTCTAGTCGTGTTAAGACCTTGTCTTCAACAGAAACTCAGCGAGGTGATATATTGATTCCTAATGCAAGGATAATTCTCTGTTTTCATGACAAAGGTGGTAAAGATGTTAGAGGCTTCTCTTCCTGGGATCAGTTTATTGCTCTTGAGTTTTCTTCGCCATCAAATTTTAACAAAGGTGTTCAAGACCATGGCCCAACTTTAGATGCAAGGTCAGAGAAAATGTATTTTTCAACAGCCACACGTTCTTTACATTTGAATGTTGGTAATCTGGACATTTTCCTCGTCAGTCCTGCAAGTAAGGATAATGCTGGGATCAGCTCTGGAAACATGCAGAGGCATACATTTGCTGCAAAAAAAATAATGTCCGCCAGCAACAGTACGGGCCGTCTATCTGTTATTAGTATGCTTTGGCAGGAGGGTTATGTGACTGGCCCTTGGATAGCAAAGAAAGCCAAGTGCTTAGCTGGTTTTGAGGAATCAAGGAGCATTTCTAAATTTGTCGGCAAAGATCATGAATTTGCTTCAGTATCTACTATTAAAGATCTGCAAGATTTGAATTCTCAAACTCAACAGGAGATTATCTTGAGCTCTGCGTTTTCCCTACATGTTTGTCTACCCTCTGTTTCAATAAATTTGGACTATTCTCAATATAAAGGTTTATATTGTCTATTAGATCAAATGTTAAATGACTTAAACTCGGACTGTGATTCAGTCAATTTTAAGGAAGCATCTTCCGCATCCCAGACATCTATCCTTGTTAGATGTGATTCTGTTGAAATTTTGATTAGCCTGGATGTGAAGGAGATTGTCAAAAGCTCAATACAGAGTGAACTTCCTGGGTCGTGGCATCAGCTGAAACTGAAAATTCAGAAGCTAGATGTGCTGTCTGTCTCCAATATTGGAGGTATATCAGGTGCCGATTTTTTCTGGCTGGCCCATGGTGAAGGCAAATTGTGGGGTTACATAACTGGAATTCCTGATCAGGAGTTTCTTCTGATTGCGTGCAGCAACTCAACAATGAAACGCGGTGATGGAGGGGGCTCTAATGCATTATCCTGCAGGCTAGCTGGTTCTGATATTGTACATTTGTGGGATCCCAAAAGTTTTCATGGTTCTACGTCTGTAACTGTCAGATGTGCCACAATAGTTGCATTAGGTGGTCGCTTGGATTGGGCAGATGCACTATGCTCTTTTTTCATTATTCCTTGTGAAAATGAAGCGGAAAAGTGTAACAAAAAGGATGAATTTAGTGCACCTCACAGATCTTCCTTTGTTCTTAACTTGGTTGATATTGGATTAAGTTATGAGCCCTACCTCAAGAATGTGGTGGTCAGGCATGAAGCTTCAGATTCTGAGCCCAATTCTTCATCTTTCAAGGAAGAGACGGGTGAGGAACATGTTTCTTGTCTTTTGGCTGCATCTTCCTTGAATCTTTCAAACTCAACCAGGGCAGGCTCAGTAGAAAATGATTACAGAATAAGAGTGCAAGATCTTGGACTTCTCCTCTGTTTAATGTCAAAGCCTGAGGATGTTGGTGGCATTTATAGTGCAGTACATCTTCACAAGATTGGGTATGTTAAAGTTGCCAGGGAGGCACTGGTAGAAGCAACTTTGAGAACTAATTGTAATAGTGGCCTTCTCTGGGAGGTAGAATGTTCAAAATCTCACATCTATGTGGAAACTTGCCATGACACCATGTCCAGTCTGTTTCGTTTAGCTGCCCAACTTCAACAGCTATTTGCCCCTGACATGGAGGAATCAGTCGTGCACTTGCAGACTAGATGGAATAAGGTACAGCAGGAACAAGAGAGCAGAGGTTTTCATGATGAAGCAAGGAACTGTGGTAGTGATCCTTTACGAGCAACTTCTCAATTGCACACTTCTGGTGCAGTTACAGAGAGTGAAACTCAGTTGGTTGGGTTAATGGATGAGATATGTGATGATGCATTTCTTTCGGATAAGAATCAGACCTGCCATTCTGAGTCGCAAATTGGTATCTCATTTGATCAAGACCTTGGAGAGGCTCATTATTCCAGCATTGCAACTCCTGATATTCTTTCACTTGGTGCACCTTATAATGGGTCAGTGCCTGAAGCAGAGTTGGAAAGTAGTCAAACATCATTTCTACAAGAAGGTACTGTTCTGAAGTTAATAGAAGGGTATTGTTTGCCTGATTTACGGCCTCTCTCGGAATTATCTGCCAACAGACAGTCATCGAATGAGATTCTAAAATGCAAATCCAAGAATTTCATAAATGGAGATGTTGGAGGAGATAGTAATGGATGGTATGGTACTTCCATTAGCATTTTAGAAAACCACATTCCTGAAGCATGTGAAAATAGTATGAAACAATCTGCCGAAGACAATCTTCCTTTTATTGAAGGTATAAAGTGCAATGATTTTGGAAAAGCCATTGGACGTGTACTTCTTAAGAACATTGATGTTAGGTGGAGAATGCTTTCTGGGTCAGACTGGCACGATTCTAGATCAACTGGTCAGCGATCAGTGGATTTTAGCGGAAGGGATGCTACTGTATGTTTAGAGCTTGCACTATCCGGAATGGAGTTTCAGTATGACATTTTTCCCGTCGGTGGAATCTGTGTATCTAAGCTCTCTCTTTCAGTTCAAGATTTTTATCTATATGACAGCAGCAAAGAGGCTCCTTGGAAACTGGTACTCGGATATTATCATTCAAAGGACCGACCTCGAAAATCATCTTCGAAAGCATTCAAGCTGGACTTAGAATCTGTCAGACCAGATCCCTTAACTCCTCTTGAAGAGTATCGGTTACGTGTTGCACTCCTTCCCATGCTATTGCATCTTCATCAGTCCCAACTTGATTTTCTCATTAACTTCTTTGCGGCAAAGAGCTCTTCAACTGATCATTCTCCGGGTGGTCATCAAGATGCTGATGGTTCAAAAGTCTTGCCGGTAAAAAGTAATGATCTTGCAGGGCATACCATTGAAGAGGAGGCATTTCTTCCTTATTTTCAGAAGTTCGATATATGGCCTATTCTAGTTCGGGTTGACTACAGTCCCAGCCGTGATTCGATCTGGCAGCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0002376 GO:0006914 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007033 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009814 GO:0009816 GO:0009987 GO:0010150 GO:0016043 GO:0016236 GO:0022607 GO:0032501 GO:0032502 GO:0042742 GO:0043207 GO:0044085 GO:0044237 GO:0044248 GO:0045087 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0061919 GO:0070925 GO:0071840 GO:0090693 GO:0098542 GO:0099402 GO:1905037

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 map04136 map04138 map04140

Pfam Domains

Protein Families

ATG2_CAD ATG_C Chorein_N

Protein Analysis

1720

Amino Acids

189.56

Weight (kDa)

5.3

Isoelectric Point (pI)

43.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG2_CAD	PF13329	19 - 1532	5.5e-49	Autophagy-related protein 2
ATG2_CAD	PF13329	1539 - 1713	8.5e-38	Autophagy-related protein 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0010386)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19190 AT3G19190 AT3G19190
fragaria_vesca	FvH4_2g06220
malus_domestica	MD05G1079500.v1.1 MD10G1093800.v1.1
prunus_persica	Prupe.8G123400_v2.0.a1 Prupe.8G123400_v2.0.a1
pyrus_communis	pycom05g07260 pycom10g07720
rosa_chinensis	RchiOBHm_Chr6g0256971
rosa_laevigata	RLG00000014666
rosa_multiflora	Rmu_co8396705.1_g000001 Rmu_sc0000659.1_g000038
rosa_roxburghii	Rroxscaffold_7G00208380
rosa_rugosa	Rorug05G0576000 Rorug05G0576100
rosa_samantha	Rh6AG094700 Rh6BG085900 Rh6CG082000 Rh6DG077700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4170
Acc16I	TGCGCA	1 cut(s)	331
Acc36I	ACCTGC	2 cut(s)	1579, 4073
Acc65I	GGTACC	1 cut(s)	1216
AccB1I	GGYRCC	4 cut(s)	152, 818, 1216, 3008
AccB7I	CCANNNNNTGG	1 cut(s)	1124
AccI	GTMKAC	1 cut(s)	2707
AccII	CGCG	4 cut(s)	20, 46, 176, 3120
AccIII	TCCGGA	2 cut(s)	1403, 4649
AcuI	CTGAAG	7 cut(s)	648, 1833, 2601, 3423, 4206, 4259, 4440
AcyI	GRCGYC	1 cut(s)	120
AdeI	CACNNNGTG	2 cut(s)	818, 4160
AflII	CTTAAG	1 cut(s)	4523
AflIII	ACRYGT	7 cut(s)	617, 1358, 2333, 2698, 3490, 4513, 4885
AhdI	GACNNNNNGTC	1 cut(s)	3790
AjiI	CACGTC	2 cut(s)	1359, 4514
AjnI	CCWGG	9 cut(s)	369, 388, 437, 1189, 2216, 2883, 2929, 3539, 3680
AloI	GAACNNNNNNTCC	2 cut(s)	23, 55
Alw21I	GWGCWC	5 cut(s)	2684, 3349, 3864, 4165, 4967
Alw26I	GTCTC	3 cut(s)	1563, 2989, 3470
Alw44I	GTGCAC	3 cut(s)	3345, 3860, 4161
AlwNI	CAGNNNCTG	3 cut(s)	1175, 3446, 4184
Aor13HI	TCCGGA	2 cut(s)	1403, 4649
AoxI	GGCC	9 cut(s)	51, 1115, 2284, 2476, 2524, 3027, 3727, 4274, 5102
ApaLI	GTGCAC	3 cut(s)	3345, 3860, 4161
ArsI	GACNNNNNNTTYG	2 cut(s)	745, 777
Asp700I	GAANNNNTTC	4 cut(s)	795, 2092, 4552, 4820
Asp718I	GGTACC	1 cut(s)	1216
AspA2I	CCTAGG	1 cut(s)	1552
AspLEI	GCGC	2 cut(s)	178, 332
AspS9I	GGNCC	6 cut(s)	51, 2285, 2476, 2525, 3028, 4792
AsuC2I	CCSGG	2 cut(s)	816, 4986
AsuII	TTCGAA	2 cut(s)	592, 4814
AsuNHI	GCTAGC	1 cut(s)	3157
AvaII	GGWCC	1 cut(s)	4792
AvrII	CCTAGG	1 cut(s)	1552
AxyI	CCTNAGG	1 cut(s)	3618
BaeGI	GKGCMC	4 cut(s)	155, 3349, 3864, 4165
BaeI	ACNNNNGTAYC	2 cut(s)	1779, 1812
BamHI	GGATCC	1 cut(s)	3187
BanI	GGYRCC	4 cut(s)	152, 818, 1216, 3008
BanII	GRGCYC	5 cut(s)	2684, 3137, 3405, 3453, 4967
BarI	GAAGNNNNNNTAC	2 cut(s)	4606, 4638
BauI	CACGAG	1 cut(s)	493
BbsI	GAAGAC	2 cut(s)	2109, 4461
Bbv12I	GWGCWC	5 cut(s)	2684, 3349, 3864, 4165, 4967
BccI	CCATC	9 cut(s)	395, 1239, 1241, 1298, 2260, 3119, 3870, 4371, 5000
BceAI	ACGGC	5 cut(s)	38, 792, 2463, 4289, 5121
BcgI	CGANNNNNNTGC	4 cut(s)	201, 235, 4029, 4063
BciT130I	CCWGG	9 cut(s)	371, 390, 439, 1191, 2218, 2885, 2931, 3541, 3682
BclI	TGATCA	3 cut(s)	3073, 4099, 4975
BcnI	CCSGG	2 cut(s)	816, 4986
BcoDI	GTCTC	3 cut(s)	1563, 2989, 3470
BfaI	CTAG	8 cut(s)	1553, 1884, 2097, 2969, 3158, 3873, 4578, 5111
BfmI	CTRYAG	2 cut(s)	3151, 5125
BfrI	CTTAAG	1 cut(s)	4523
BfuAI	ACCTGC	2 cut(s)	1579, 4073
BglI	GCCNNNNNGGC	1 cut(s)	3686
BglII	AGATCT	4 cut(s)	2090, 2635, 3356, 3583
BlnI	CCTAGG	1 cut(s)	1552
BlpI	GCTNAGC	1 cut(s)	2554
BmcAI	AGTACT	1 cut(s)	1888
Bme18I	GGWCC	1 cut(s)	4792
BmeRI	GACNNNNNGTC	1 cut(s)	3790
BmgBI	CACGTC	2 cut(s)	1359, 4514
BmgT120I	GGNCC	6 cut(s)	51, 2285, 2476, 2525, 3028, 4792
BmiI	GGNNCC	8 cut(s)	154, 250, 820, 1218, 1803, 3010, 3189, 4755
BmtI	GCTAGC	1 cut(s)	3161
BoxI	GACNNNNGTC	1 cut(s)	5127
BpiI	GAAGAC	2 cut(s)	2109, 4461
BplI	GAGNNNNNCTC	2 cut(s)	3985, 4017
BpmI	CTGGAG	1 cut(s)	329
Bpu10I	CCTNAGC	1 cut(s)	1425
Bpu1102I	GCTNAGC	1 cut(s)	2554
Bpu14I	TTCGAA	2 cut(s)	592, 4814
BpuEI	CTTGAG	6 cut(s)	740, 1584, 1636, 2258, 2698, 3395
BpuMI	CCSGG	2 cut(s)	816, 4986
BsaAI	YACGTR	2 cut(s)	505, 4886
BsaBI	GATNNNNATC	2 cut(s)	4056, 4728
BsaHI	GRCGYC	1 cut(s)	120
BsaWI	WCCGGW	2 cut(s)	1403, 4649
BsaXI	ACNNNNNCTCC	6 cut(s)	23, 53, 302, 332, 4649, 4679
Bse118I	RCCGGY	1 cut(s)	5023
Bse21I	CCTNAGG	1 cut(s)	3618
Bse3DI	GCAATG	2 cut(s)	4131, 4495
Bse8I	GATNNNNATC	2 cut(s)	4056, 4728
BseAI	TCCGGA	2 cut(s)	1403, 4649
BseBI	CCWGG	9 cut(s)	371, 390, 439, 1191, 2218, 2885, 2931, 3541, 3682
BseJI	GATNNNNATC	2 cut(s)	4056, 4728
BseMI	GCAATG	2 cut(s)	4131, 4495
BseRI	GAGGAG	4 cut(s)	3587, 4377, 4854, 5078
BseSI	GKGCMC	4 cut(s)	155, 3349, 3864, 4165
BseYI	CCCAGC	4 cut(s)	962, 1466, 2402, 5132
BsgI	GTGCAG	5 cut(s)	231, 1578, 3118, 3663, 4002
Bsh1236I	CGCG	4 cut(s)	20, 46, 176, 3120
BshFI	GGCC	9 cut(s)	53, 1117, 2286, 2478, 2526, 3029, 3729, 4276, 5104
BshNI	GGYRCC	4 cut(s)	152, 818, 1216, 3008
BsiHKAI	GWGCWC	5 cut(s)	2684, 3349, 3864, 4165, 4967
BsiSI	CCGG	5 cut(s)	815, 1404, 4650, 4985, 5024
BslFI	GGGAC	4 cut(s)	406, 1924, 4909, 5115
BsmAI	GTCTC	3 cut(s)	1563, 2989, 3470
BsmBI	CGTCTC	1 cut(s)	3470
BsmFI	GGGAC	4 cut(s)	406, 1924, 4909, 5115
BsmI	GAATGC	4 cut(s)	270, 1568, 4554, 4820
BsnI	GGCC	9 cut(s)	53, 1117, 2286, 2478, 2526, 3029, 3729, 4276, 5104
Bsp119I	TTCGAA	2 cut(s)	592, 4814
Bsp1286I	GDGCHC	9 cut(s)	155, 2684, 3137, 3349, 3405, 3453, 3864, 4165, 4967
Bsp13I	TCCGGA	2 cut(s)	1403, 4649
Bsp1407I	TGTACA	1 cut(s)	3175
Bsp1720I	GCTNAGC	1 cut(s)	2554
Bsp19I	CCATGG	2 cut(s)	2281, 3031
Bsp68I	TCGCGA	1 cut(s)	20
BspANI	GGCC	9 cut(s)	53, 1117, 2286, 2478, 2526, 3029, 3729, 4276, 5104
BspEI	TCCGGA	2 cut(s)	1403, 4649
BspFNI	CGCG	4 cut(s)	20, 46, 176, 3120
BspHI	TCATGA	3 cut(s)	2179, 2605, 3916
BspLI	GGNNCC	8 cut(s)	154, 250, 820, 1218, 1803, 3010, 3189, 4755
BspMAI	CTGCAG	1 cut(s)	3155
BspMI	ACCTGC	2 cut(s)	1579, 4073
BspOI	GCTAGC	1 cut(s)	3161
BspQI	GCTCTTC	1 cut(s)	4972
BspT104I	TTCGAA	2 cut(s)	592, 4814
BspT107I	GGYRCC	4 cut(s)	152, 818, 1216, 3008
BspTI	CTTAAG	1 cut(s)	4523
BsrDI	GCAATG	2 cut(s)	4131, 4495
BsrFI	RCCGGY	1 cut(s)	5023
BsrGI	TGTACA	1 cut(s)	3175
BssAI	RCCGGY	1 cut(s)	5023
BssNI	GRCGYC	1 cut(s)	120
BssSI	CACGAG	1 cut(s)	493
BssT1I	CCWWGG	9 cut(s)	269, 884, 1082, 1552, 2281, 2528, 3031, 4108, 4757
Bst2BI	CACGAG	1 cut(s)	493
Bst2UI	CCWGG	9 cut(s)	371, 390, 439, 1191, 2218, 2885, 2931, 3541, 3682
Bst6I	CTCTTC	6 cut(s)	53, 2218, 3468, 4866, 4972, 5055
BstACI	GRCGYC	1 cut(s)	120
BstAFI	CTTAAG	1 cut(s)	4523
BstAPI	GCANNNNNTGC	3 cut(s)	419, 431, 765
BstAUI	TGTACA	1 cut(s)	3175
BstBAI	YACGTR	2 cut(s)	505, 4886
BstBI	TTCGAA	2 cut(s)	592, 4814
BstC8I	GCNNGC	9 cut(s)	805, 1038, 2464, 3027, 3097, 3155, 3159, 3547, 4641
BstDSI	CCRYGG	3 cut(s)	8, 2281, 3031
BstENI	CCTNNNNNAGG	2 cut(s)	374, 4112
BstFNI	CGCG	4 cut(s)	20, 46, 176, 3120
BstHHI	GCGC	2 cut(s)	178, 332
BstMAI	GTCTC	3 cut(s)	1563, 2989, 3470
BstNI	CCWGG	9 cut(s)	371, 390, 439, 1191, 2218, 2885, 2931, 3541, 3682
BstNSI	RCATGY	7 cut(s)	759, 1418, 1439, 2425, 2702, 3494, 4428
BstPAI	GACNNNNGTC	1 cut(s)	5127
BstSFI	CTRYAG	2 cut(s)	3151, 5125
BstSLI	GKGCMC	4 cut(s)	155, 3349, 3864, 4165
BstUI	CGCG	4 cut(s)	20, 46, 176, 3120
BstV2I	GAAGAC	2 cut(s)	2109, 4461
BstX2I	RGATCY	9 cut(s)	239, 1406, 2080, 2090, 2635, 3187, 3356, 3583, 4852
BstYI	RGATCY	9 cut(s)	239, 1406, 2080, 2090, 2635, 3187, 3356, 3583, 4852
Bsu36I	CCTNAGG	1 cut(s)	3618
BsuRI	GGCC	9 cut(s)	53, 1117, 2286, 2478, 2526, 3029, 3729, 4276, 5104
BtgI	CCRYGG	3 cut(s)	8, 2281, 3031
BtgZI	GCGATG	2 cut(s)	50, 699
BtrI	CACGTC	2 cut(s)	1359, 4514
BtsI	GCAGTG	2 cut(s)	1371, 1763
BtuMI	TCGCGA	1 cut(s)	20
BveI	ACCTGC	2 cut(s)	1579, 4073
Cac8I	GCNNGC	9 cut(s)	805, 1038, 2464, 3027, 3097, 3155, 3159, 3547, 4641
CaiI	CAGNNNCTG	3 cut(s)	1175, 3446, 4184
CciI	TCATGA	3 cut(s)	2179, 2605, 3916
CfoI	GCGC	2 cut(s)	178, 332
Cfr10I	RCCGGY	1 cut(s)	5023
Cfr13I	GGNCC	6 cut(s)	51, 2285, 2476, 2525, 3028, 4792
CseI	GACGC	2 cut(s)	128, 1516
CspCI	CAANNNNNGTGG	2 cut(s)	3223, 3258
DraIII	CACNNNGTG	2 cut(s)	818, 4160
DriI	GACNNNNNGTC	1 cut(s)	3790
Eam1104I	CTCTTC	6 cut(s)	53, 2218, 3468, 4866, 4972, 5055
Eam1105I	GACNNNNNGTC	1 cut(s)	3790
EarI	CTCTTC	6 cut(s)	53, 2218, 3468, 4866, 4972, 5055
EciI	GGCGGA	2 cut(s)	44, 2449
Ecl136II	GAGCTC	2 cut(s)	2682, 4965
Eco130I	CCWWGG	9 cut(s)	269, 884, 1082, 1552, 2281, 2528, 3031, 4108, 4757
Eco24I	GRGCYC	5 cut(s)	2684, 3137, 3405, 3453, 4967
Eco47I	GGWCC	1 cut(s)	4792
Eco53kI	GAGCTC	2 cut(s)	2682, 4965
Eco57I	CTGAAG	7 cut(s)	648, 1833, 2601, 3423, 4206, 4259, 4440
Eco81I	CCTNAGG	1 cut(s)	3618
EcoICRI	GAGCTC	2 cut(s)	2682, 4965
EcoNI	CCTNNNNNAGG	2 cut(s)	374, 4112
EcoRI	GAATTC	2 cut(s)	2650, 3066
EcoRII	CCWGG	9 cut(s)	369, 388, 437, 1189, 2216, 2883, 2929, 3539, 3680
EcoT14I	CCWWGG	9 cut(s)	269, 884, 1082, 1552, 2281, 2528, 3031, 4108, 4757
EcoT22I	ATGCAT	2 cut(s)	3145, 4042
EcoT38I	GRGCYC	5 cut(s)	2684, 3137, 3405, 3453, 4967
ErhI	CCWWGG	9 cut(s)	269, 884, 1082, 1552, 2281, 2528, 3031, 4108, 4757
Esp3I	CGTCTC	1 cut(s)	3470
FalI	AAGNNNNNCTT	6 cut(s)	868, 900, 4446, 4478, 4817, 4849
FaqI	GGGAC	4 cut(s)	406, 1924, 4909, 5115
FauI	CCCGC	1 cut(s)	1901
FbaI	TGATCA	3 cut(s)	3073, 4099, 4975
FblI	GTMKAC	1 cut(s)	2707
FriOI	GRGCYC	5 cut(s)	2684, 3137, 3405, 3453, 4967
FspBI	CTAG	8 cut(s)	1553, 1884, 2097, 2969, 3158, 3873, 4578, 5111
FspI	TGCGCA	1 cut(s)	331
GlaI	GCGC	2 cut(s)	177, 331
GsaI	CCCAGC	4 cut(s)	966, 1470, 2406, 5136
GsuI	CTGGAG	1 cut(s)	329
HaeIII	GGCC	9 cut(s)	53, 1117, 2286, 2478, 2526, 3029, 3729, 4276, 5104
HapII	CCGG	5 cut(s)	815, 1404, 4650, 4985, 5024
HgaI	GACGC	2 cut(s)	128, 1516
HhaI	GCGC	2 cut(s)	178, 332
Hin1I	GRCGYC	1 cut(s)	120
Hin6I	GCGC	2 cut(s)	176, 330
HinP1I	GCGC	2 cut(s)	176, 330
HincII	GTYRAC	1 cut(s)	5122
HindII	GTYRAC	1 cut(s)	5122
HindIII	AAGCTT	2 cut(s)	2060, 3435
HpaII	CCGG	5 cut(s)	815, 1404, 4650, 4985, 5024
Hpy99I	CGWCG	3 cut(s)	122, 188, 4685
HpyCH4IV	ACGT	9 cut(s)	183, 395, 504, 617, 1358, 2335, 3212, 4513, 4885
HpySE526I	ACGT	9 cut(s)	183, 395, 504, 617, 1358, 2335, 3212, 4513, 4885
Hsp92I	GRCGYC	1 cut(s)	120
HspAI	GCGC	2 cut(s)	176, 330
Kpn2I	TCCGGA	2 cut(s)	1403, 4649
KpnI	GGTACC	1 cut(s)	1220
Ksp22I	TGATCA	3 cut(s)	3073, 4099, 4975
LguI	GCTCTTC	1 cut(s)	4972
LmnI	GCTCC	5 cut(s)	254, 310, 710, 2577, 4759
MaeI	CTAG	8 cut(s)	1553, 1884, 2097, 2969, 3158, 3873, 4578, 5111
MaeII	ACGT	9 cut(s)	183, 395, 504, 617, 1358, 2335, 3212, 4513, 4885
MfeI	CAATTG	3 cut(s)	465, 683, 3965
MflI	RGATCY	9 cut(s)	239, 1406, 2080, 2090, 2635, 3187, 3356, 3583, 4852
MhlI	GDGCHC	9 cut(s)	155, 2684, 3137, 3349, 3405, 3453, 3864, 4165, 4967
MlyI	GAGTC	2 cut(s)	2054, 4085
MmeI	TCCRAC	3 cut(s)	1160, 4176, 4339
Mph1103I	ATGCAT	2 cut(s)	3145, 4042
MroI	TCCGGA	2 cut(s)	1403, 4649
MroXI	GAANNNNTTC	4 cut(s)	795, 2092, 4552, 4820
MslI	CAYNNNNRTG	7 cut(s)	453, 568, 1515, 2349, 3765, 3767, 4998
MspA1I	CMGCKG	3 cut(s)	1040, 1385, 2947
MspCI	CTTAAG	1 cut(s)	4523
MspI	CCGG	5 cut(s)	815, 1404, 4650, 4985, 5024
MunI	CAATTG	3 cut(s)	465, 683, 3965
Mva1269I	GAATGC	4 cut(s)	270, 1568, 4554, 4820
MvaI	CCWGG	9 cut(s)	371, 390, 439, 1191, 2218, 2885, 2931, 3541, 3682
MvnI	CGCG	4 cut(s)	20, 46, 176, 3120
NciI	CCSGG	2 cut(s)	816, 4986
NcoI	CCATGG	2 cut(s)	2281, 3031
NheI	GCTAGC	1 cut(s)	3157
NlaIV	GGNNCC	8 cut(s)	154, 250, 820, 1218, 1803, 3010, 3189, 4755
NmeAIII	GCCGAG	1 cut(s)	1592
NmuCI	GTSAC	4 cut(s)	110, 404, 1194, 2518
NruI	TCGCGA	1 cut(s)	20
NsbI	TGCGCA	1 cut(s)	331
NsiI	ATGCAT	2 cut(s)	3145, 4042
NspI	RCATGY	7 cut(s)	759, 1418, 1439, 2425, 2702, 3494, 4428
NspV	TTCGAA	2 cut(s)	592, 4814
PagI	TCATGA	3 cut(s)	2179, 2605, 3916
PasI	CCCWGGG	1 cut(s)	389
PciI	ACATGT	2 cut(s)	2698, 3490
PciSI	GCTCTTC	1 cut(s)	4972
PcsI	WCGNNNNNNNCGW	2 cut(s)	32, 180
PctI	GAATGC	4 cut(s)	270, 1568, 4554, 4820
PdmI	GAANNNNTTC	4 cut(s)	795, 2092, 4552, 4820
PflFI	GACNNNGTC	1 cut(s)	2113
PflMI	CCANNNNNTGG	1 cut(s)	1124
PleI	GAGTC	2 cut(s)	2053, 4084
PpsI	GAGTC	2 cut(s)	2053, 4084
Ppu21I	YACGTR	2 cut(s)	505, 4886
PscI	ACATGT	2 cut(s)	2698, 3490
PshAI	GACNNNNGTC	1 cut(s)	5127
PsiI	TTATAA	1 cut(s)	4170
Psp124BI	GAGCTC	2 cut(s)	2684, 4967
Psp6I	CCWGG	9 cut(s)	369, 388, 437, 1189, 2216, 2883, 2929, 3539, 3680
PspFI	CCCAGC	4 cut(s)	962, 1466, 2402, 5132
PspGI	CCWGG	9 cut(s)	369, 388, 437, 1189, 2216, 2883, 2929, 3539, 3680
PspN4I	GGNNCC	8 cut(s)	154, 250, 820, 1218, 1803, 3010, 3189, 4755
PspPI	GGNCC	6 cut(s)	51, 2285, 2476, 2525, 3028, 4792
PstI	CTGCAG	1 cut(s)	3155
PstNI	CAGNNNCTG	3 cut(s)	1175, 3446, 4184
PsuI	RGATCY	9 cut(s)	239, 1406, 2080, 2090, 2635, 3187, 3356, 3583, 4852
PsyI	GACNNNGTC	1 cut(s)	2113
PvuII	CAGCTG	2 cut(s)	1385, 2947
RruI	TCGCGA	1 cut(s)	20
RseI	CAYNNNNRTG	7 cut(s)	453, 568, 1515, 2349, 3765, 3767, 4998
SacI	GAGCTC	2 cut(s)	2684, 4967
SapI	GCTCTTC	1 cut(s)	4972
Sau96I	GGNCC	6 cut(s)	51, 2285, 2476, 2525, 3028, 4792
SbfI	CCTGCAGG	1 cut(s)	3155
ScaI	AGTACT	1 cut(s)	1888
SchI	GAGTC	2 cut(s)	2054, 4085
SdaI	CCTGCAGG	1 cut(s)	3155
SduI	GDGCHC	9 cut(s)	155, 2684, 3137, 3349, 3405, 3453, 3864, 4165, 4967
SfcI	CTRYAG	2 cut(s)	3151, 5125
SfuI	TTCGAA	2 cut(s)	592, 4814
SinI	GGWCC	1 cut(s)	4792
SmiMI	CAYNNNNRTG	7 cut(s)	453, 568, 1515, 2349, 3765, 3767, 4998
SmlI	CTYRAG	7 cut(s)	719, 1599, 1651, 2237, 2677, 3410, 4523
SmoI	CTYRAG	7 cut(s)	719, 1599, 1651, 2237, 2677, 3410, 4523
Sse8387I	CCTGCAGG	1 cut(s)	3155
SspI	AATATT	3 cut(s)	834, 1705, 2992
SspMI	CTAG	8 cut(s)	1553, 1884, 2097, 2969, 3158, 3873, 4578, 5111
SstI	GAGCTC	2 cut(s)	2684, 4967
StyI	CCWWGG	9 cut(s)	269, 884, 1082, 1552, 2281, 2528, 3031, 4108, 4757
TaiI	ACGT	9 cut(s)	186, 398, 507, 620, 1361, 2338, 3215, 4516, 4888
TaqII	GACCGA	1 cut(s)	4809
TatI	WGTACW	4 cut(s)	1886, 3175, 3646, 4516
TauI	GCSGC	1 cut(s)	4958
TseFI	GTSAC	4 cut(s)	110, 404, 1194, 2518
Tsp45I	GTSAC	4 cut(s)	110, 404, 1194, 2518
Tth111I	GACNNNGTC	1 cut(s)	2113
Van91I	CCANNNNNTGG	1 cut(s)	1124
Vha464I	CTTAAG	1 cut(s)	4523
VneI	GTGCAC	3 cut(s)	3345, 3860, 4161
VpaK11BI	GGWCC	1 cut(s)	4792
XagI	CCTNNNNNAGG	2 cut(s)	374, 4112
XbaI	TCTAGA	1 cut(s)	4577
XceI	RCATGY	7 cut(s)	759, 1418, 1439, 2425, 2702, 3494, 4428
XcmI	CCANNNNNNNNNTGG	2 cut(s)	832, 3688
XmaJI	CCTAGG	1 cut(s)	1552
XmiI	GTMKAC	1 cut(s)	2707
XmnI	GAANNNNTTC	4 cut(s)	795, 2092, 4552, 4820
XspI	CTAG	8 cut(s)	1553, 1884, 2097, 2969, 3158, 3873, 4578, 5111
ZrmI	AGTACT	1 cut(s)	1888
Zsp2I	ATGCAT	2 cut(s)	3145, 4042

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.