pycom05g12690

Belongs to the expansin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013081
Physical Location & Seq
Forward (+)
16139263 .. 16140703
1441 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12690.1

Sequence Viewer

Length: 783 bp
ATGTCTGTGTTTATTTGCTTCCTCTTCTTCTTCCTAATCTCCTCTGCAACCGCTTGTGATCGCTGTGTGAAGCAATCCAAGGCAGCCTTCTTCTCCAAATCCTCTGCACTTTCATCTGGGGCTTGTGGGTATGGCTCCTTGGCCTTGGGATTTAGTGGCGGACACCTTGCAGCTGGTGTTCCTTCCCTTTTCAAAGGTGGAGCTGGTTGTGGTGCATGCTTTCAGATAAGATGCAAGAATACAACTCTTTGTACAAGACATGGGACTATAGTAACTTTGACTGATATGAACCAAGGCAACCAAACGGATTTTGTTCTCAGCAGCAGAGCTTTTGCGGCCATGGCTCAAAAGGGTTTGGGCCAAGACATTTTGAGACGTGGCATTGTGGATGTGGAGTATAAGAGGGTACCATGTGAGTACAAGAACCAAAATTTATCCTTACGTGTGGAAGAATCAAGCCAAAAGCCACATTATTTGGCAATCAAACTTTTGTACCAAGGTGGTCAGACAGAAATTGTAGGCATTGATGTTGCTCAGGTTGGATCTTCAAATTGGAGTTTCCTATCTCGAAACCACGGTTCGATCTGGGACACGAGTAGGGTTCCGGCTGGGGCGCTACAATTCCGGTTTGTGGTGACGGCTGGGTACGATGGGAAGACGGTTTGGGCACCGAATGTGCTTCCCGTTAACTGGAAGCCCGGGATGATATATGACACAAAGGTTCAAATCAGTGACATTGCACAAGAGGGTTGCTCTCACTGTGATGATGGGAGCTGGAAATGA

Protein Analysis

261

Amino Acids

28.11

Weight (kDa)

8.86

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 57 - 133 1.8e-13 Lytic transglycolase
Expansin_C PF01357 147 - 228 2.9e-17 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45970 AT3G45970 AT4G38400
fragaria_vesca FvH4_2g16110
malus_domestica MD05G1130300.v1.1 MD10G1133200.v1.1
prunus_persica Prupe.8G174500_v2.0.a1
pyrus_communis pycom05g12690 pycom10g11500
rosa_chinensis RchiOBHm_Chr6g0279781
rosa_laevigata RLG00000013120
rosa_multiflora Rmu_sc0001719.1_g000021
rosa_roxburghii Rroxscaffold_7G00188660
rosa_rugosa Rorug06G0128000
rosa_samantha Rh6AG236200 Rh6BG240400 Rh6DG234100
rosa_wichuraiana Rw6G020590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 406
AccB1I GGYRCC 2 cut(s) 406, 667
AciI CCGC 3 cut(s) 51, 159, 335
AclWI GGATC 1 cut(s) 550
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 1 cut(s) 430
AfaI GTAC 5 cut(s) 253, 408, 419, 494, 647
AfiI CCNNNNNNNGG 2 cut(s) 631, 690
AflIII ACRYGT 1 cut(s) 442
AgsI TTSAA 3 cut(s) 193, 549, 725
AjiI CACGTC 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 4 cut(s) 173, 203, 329, 774
AluI AGCT 4 cut(s) 173, 203, 329, 774
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 1 cut(s) 550
Ama87I CYCGRG 1 cut(s) 698
AoxI GGCC 3 cut(s) 141, 336, 358
ApeKI GCWGC 3 cut(s) 83, 170, 321
ApoI RAATTY 1 cut(s) 430
Asp718I GGTACC 1 cut(s) 406
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 2 cut(s) 699, 700
AsuHPI GGTGA 1 cut(s) 646
AvaI CYCGRG 1 cut(s) 698
BaeGI GKGCMC 1 cut(s) 670
BaeI ACNNNNGTAYC 2 cut(s) 476, 509
BanI GGYRCC 2 cut(s) 406, 667
BauI CACGAG 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 662
BbvI GCAGC 3 cut(s) 95, 182, 333
BccI CCATC 2 cut(s) 644, 761
BceAI ACGGC 1 cut(s) 654
BcnI CCSGG 2 cut(s) 699, 700
BcoDI GTCTC 1 cut(s) 367
BfmI CTRYAG 1 cut(s) 267
BfoI RGCGCY 1 cut(s) 617
BisI GCNGC 4 cut(s) 84, 171, 322, 336
BlsI GCNGC 4 cut(s) 85, 172, 323, 337
Bme1390I CCNGG 2 cut(s) 699, 700
BmeT110I CYCGRG 1 cut(s) 698
BmgBI CACGTC 1 cut(s) 377
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 4 cut(s) 136, 408, 603, 669
BmrFI CCNGG 2 cut(s) 699, 700
BmsI GCATC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 662
BplI GAGNNNNNCTC 2 cut(s) 737, 769
Bpu10I CCTNAGC 1 cut(s) 534
BpuMI CCSGG 2 cut(s) 699, 700
BsaAI YACGTR 1 cut(s) 443
BsaJI CCNNGG 8 cut(s) 78, 138, 144, 292, 339, 496, 574, 698
BsaWI WCCGGW 1 cut(s) 624
Bsc4I CCNNNNNNNGG 2 cut(s) 631, 690
Bse1I ACTGG 1 cut(s) 695
Bse3DI GCAATG 1 cut(s) 735
BseDI CCNNGG 8 cut(s) 78, 138, 144, 292, 339, 496, 574, 698
BseGI GGATG 2 cut(s) 394, 708
BseLI CCNNNNNNNGG 2 cut(s) 631, 690
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 2 cut(s) 331, 548
BseNI ACTGG 1 cut(s) 695
BseRI GAGGAG 1 cut(s) 31
BseSI GKGCMC 1 cut(s) 670
BseXI GCAGC 3 cut(s) 95, 182, 333
BseYI CCCAGC 2 cut(s) 608, 641
BsgI GTGCAG 1 cut(s) 90
BshFI GGCC 3 cut(s) 143, 338, 360
BshNI GGYRCC 2 cut(s) 406, 667
BsiHKCI CYCGRG 1 cut(s) 698
BsiSI CCGG 3 cut(s) 605, 625, 699
BslFI GGGAC 2 cut(s) 277, 602
BslI CCNNNNNNNGG 2 cut(s) 631, 690
BsmAI GTCTC 1 cut(s) 367
BsmBI CGTCTC 1 cut(s) 367
BsmFI GGGAC 2 cut(s) 277, 602
BsnI GGCC 3 cut(s) 143, 338, 360
BsoBI CYCGRG 1 cut(s) 698
Bsp1286I GDGCHC 1 cut(s) 670
Bsp1407I TGTACA 1 cut(s) 251
Bsp143I GATC 3 cut(s) 58, 542, 582
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 3 cut(s) 51, 159, 335
BspANI GGCC 3 cut(s) 143, 338, 360
BspCNI CTCAG 2 cut(s) 330, 547
BspLI GGNNCC 4 cut(s) 136, 408, 603, 669
BspPI GGATC 1 cut(s) 550
BspT107I GGYRCC 2 cut(s) 406, 667
BsrDI GCAATG 1 cut(s) 735
BsrGI TGTACA 1 cut(s) 251
BsrI ACTGG 1 cut(s) 695
BssECI CCNNGG 8 cut(s) 78, 138, 144, 292, 339, 496, 574, 698
BssMI GATC 3 cut(s) 58, 542, 582
BssSI CACGAG 1 cut(s) 592
BssT1I CCWWGG 6 cut(s) 78, 138, 144, 292, 339, 496
Bst2BI CACGAG 1 cut(s) 592
Bst4CI ACNGT 3 cut(s) 578, 661, 761
Bst6I CTCTTC 1 cut(s) 29
BstAUI TGTACA 1 cut(s) 251
BstBAI YACGTR 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 2 cut(s) 317, 534
BstDSI CCRYGG 2 cut(s) 339, 574
BstF5I GGATG 2 cut(s) 394, 708
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 3 cut(s) 61, 545, 585
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 3 cut(s) 58, 542, 582
BstMWI GCNNNNNNNGC 2 cut(s) 335, 341
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 2 cut(s) 697, 698
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 670
BstV1I GCAGC 3 cut(s) 95, 182, 333
BstV2I GAAGAC 1 cut(s) 662
BstX2I RGATCY 1 cut(s) 542
BstYI RGATCY 1 cut(s) 542
BsuRI GGCC 3 cut(s) 143, 338, 360
BtgI CCRYGG 2 cut(s) 339, 574
BtrI CACGTC 1 cut(s) 377
BtsCI GGATG 2 cut(s) 394, 708
BtsIMutI CAGTG 2 cut(s) 736, 757
Cac8I GCNNGC 1 cut(s) 217
CfoI GCGC 1 cut(s) 616
Cfr13I GGNCC 1 cut(s) 358
Cfr9I CCCGGG 1 cut(s) 698
Csp6I GTAC 5 cut(s) 252, 407, 418, 493, 646
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 4 cut(s) 216, 260, 340, 411
CviQI GTAC 5 cut(s) 252, 407, 418, 493, 646
DdeI CTNAG 2 cut(s) 317, 534
DpnI GATC 3 cut(s) 60, 544, 584
DpnII GATC 3 cut(s) 58, 542, 582
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 174
Eco130I CCWWGG 6 cut(s) 78, 138, 144, 292, 339, 496
Eco88I CYCGRG 1 cut(s) 698
EcoT14I CCWWGG 6 cut(s) 78, 138, 144, 292, 339, 496
ErhI CCWWGG 6 cut(s) 78, 138, 144, 292, 339, 496
Esp3I CGTCTC 1 cut(s) 367
FaeI CATG 4 cut(s) 219, 263, 343, 414
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 2 cut(s) 277, 602
FatI CATG 4 cut(s) 215, 259, 339, 410
Fnu4HI GCNGC 4 cut(s) 84, 171, 322, 336
FokI GGATG 2 cut(s) 401, 715
Fsp4HI GCNGC 4 cut(s) 84, 171, 322, 336
GlaI GCGC 1 cut(s) 615
GluI GCNGC 4 cut(s) 84, 171, 322, 336
GsaI CCCAGC 2 cut(s) 612, 645
HaeII RGCGCY 1 cut(s) 617
HaeIII GGCC 3 cut(s) 143, 338, 360
HapII CCGG 3 cut(s) 605, 625, 699
HhaI GCGC 1 cut(s) 616
Hin1II CATG 4 cut(s) 219, 263, 343, 414
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HinfI GANTC 1 cut(s) 452
HpaI GTTAAC 1 cut(s) 688
HpaII CCGG 3 cut(s) 605, 625, 699
HphI GGTGA 1 cut(s) 646
Hpy166II GTNNAC 1 cut(s) 688
Hpy188I TCNGA 2 cut(s) 225, 507
Hpy188III TCNNGA 1 cut(s) 567
Hpy8I GTNNAC 1 cut(s) 688
HpyAV CCTTC 2 cut(s) 97, 192
HpyCH4III ACNGT 3 cut(s) 578, 661, 761
HpyCH4IV ACGT 2 cut(s) 376, 442
HpyCH4V TGCA 6 cut(s) 47, 107, 170, 215, 234, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 341
HpyF3I CTNAG 2 cut(s) 317, 534
HpySE526I ACGT 2 cut(s) 376, 442
Hsp92II CATG 4 cut(s) 219, 263, 343, 414
HspAI GCGC 1 cut(s) 614
KpnI GGTACC 1 cut(s) 410
KspAI GTTAAC 1 cut(s) 688
Kzo9I GATC 3 cut(s) 58, 542, 582
LmnI GCTCC 3 cut(s) 140, 200, 771
Lsp1109I GCAGC 3 cut(s) 95, 182, 333
LweI GCATC 1 cut(s) 221
MaeII ACGT 2 cut(s) 376, 442
MaeIII GTNAC 3 cut(s) 271, 634, 731
MalI GATC 3 cut(s) 60, 544, 584
MboI GATC 3 cut(s) 58, 542, 582
MboII GAAGA 7 cut(s) 16, 19, 22, 82, 461, 537, 667
MflI RGATCY 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 670
MluCI AATT 4 cut(s) 430, 513, 550, 620
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 5 cut(s) 32, 52, 112, 396, 739
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 1 cut(s) 762
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 3 cut(s) 605, 625, 699
MspR9I CCNGG 2 cut(s) 699, 700
MwoI GCNNNNNNNGC 2 cut(s) 335, 341
NciI CCSGG 2 cut(s) 699, 700
NcoI CCATGG 1 cut(s) 339
NdeII GATC 3 cut(s) 58, 542, 582
NlaIII CATG 4 cut(s) 219, 263, 343, 414
NlaIV GGNNCC 4 cut(s) 136, 408, 603, 669
NmuCI GTSAC 2 cut(s) 634, 731
NspI RCATGY 1 cut(s) 219
PaeI GCATGC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 452
PkrI GCNGC 4 cut(s) 85, 172, 323, 337
Ppu21I YACGTR 1 cut(s) 443
PspFI CCCAGC 2 cut(s) 608, 641
PspN4I GGNNCC 4 cut(s) 136, 408, 603, 669
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 5 cut(s) 253, 408, 419, 494, 647
RsaNI GTAC 5 cut(s) 252, 407, 418, 493, 646
RseI CAYNNNNRTG 1 cut(s) 762
SaqAI TTAA 1 cut(s) 687
SatI GCNGC 4 cut(s) 84, 171, 322, 336
Sau3AI GATC 3 cut(s) 58, 542, 582
Sau96I GGNCC 1 cut(s) 358
ScrFI CCNGG 2 cut(s) 699, 700
SduI GDGCHC 1 cut(s) 670
SfaNI GCATC 1 cut(s) 221
SfcI CTRYAG 1 cut(s) 267
SmaI CCCGGG 1 cut(s) 700
SmiMI CAYNNNNRTG 1 cut(s) 762
SphI GCATGC 1 cut(s) 219
Sse9I AATT 4 cut(s) 430, 513, 550, 620
SsiI CCGC 3 cut(s) 51, 159, 335
StyD4I CCNGG 2 cut(s) 697, 698
StyI CCWWGG 6 cut(s) 78, 138, 144, 292, 339, 496
TaaI ACNGT 3 cut(s) 578, 661, 761
TaiI ACGT 2 cut(s) 379, 445
TaqI TCGA 2 cut(s) 568, 581
TasI AATT 4 cut(s) 430, 513, 550, 620
TatI WGTACW 2 cut(s) 251, 417
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 2 cut(s) 736, 764
TseFI GTSAC 2 cut(s) 634, 731
TseI GCWGC 3 cut(s) 83, 170, 321
Tsp45I GTSAC 2 cut(s) 634, 731
TspDTI ATGAA 2 cut(s) 102, 302
TspGWI ACGGA 1 cut(s) 320
TspMI CCCGGG 1 cut(s) 698
TspRI CASTG 2 cut(s) 736, 764
XapI RAATTY 1 cut(s) 430
XceI RCATGY 1 cut(s) 219
XmaI CCCGGG 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.