Rorug06G0128000

Belongs to the expansin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013081
Physical Location & Seq
Reverse (-)
17780784 .. 17782922
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0128000.1

Sequence Viewer

Length: 1026 bp
ATGGTATTACTATCATCCACTCGTCTAGTCCTCCTTTCATTTGCAACGCTTTTTCTCGCTTTCTTTCTCCGACGACGACTCCTGTTCCCGGATCCAATGGCGCAAACCAAGTCCAAGATTCTCTTCATCGGAGGCACTGGCTACATTGGTAAGTTCATAGTCGAGGCCAGCGCCAAGGCCGGCCACCCCACCTTCGCTCTCGTCCGGGAGCCCACTCTCTCCAACCCGGCCAAGTCCAAAGTCATCGACAGCTTCAAGGCCTCCGGCGTCAATTTCGTCCTGGGTGATCTGTACGACCACGAGAGCCTTGTGAAGGCGATTAAGCAAGTCGATGTGGTGATTTCGACGGTGGGTCACGCGCAGTTGGCTGATCAAGGCAAGATCATCGCCGCCATTAAAGAAGCCGGCAATGTCAAGAGGTTTTTCCCTTCCGAGTTTGGAAACGACGTGGATCGAGTCCACGCTGTGGAGCCAGCAAAGACGGCGTTCGCTACGAAAGCCAAGGTCCGCAGAGATGTTGAGGCGGCCGGGATTCCCTACACTTATGTGTCATCAAACTTCTTTGCTGGTTACTTCTTGCCCAGTCTGAACCAGCCCGGAGCTACTGCTCCTCCCAGGGACAAAGTCGTCATTTTGGGTGATGGCAACCCCAAAGCAATCTTTAACAAGGAAGAGGATATTGGTACCTATACCATTAAGGCTGTGGATGAACCAAGAACATTGAACAAGATCCTCTACATCCGGCCACCTGCCAACACCTTCTCCTTCAACGAACTTGTGGCTCTTTGGGAGAAGAAGATTGGAAAAACCCTCGAGAAGGTCTATGTCCCGGAGGAGCAACTACTCAAAAACATTCAAGAGGCTGCGGTTCCAATCAATGTGATACTGGCAATCGGTCACTCAGTTTTTGTGAAAGGAGACCACACTAACTTTGAGATCGAGCCCTCATTTGGAGTGGAAGCTTCTGCTCTTTACCCTGATGTCAAATACACTACTGTGGATGAGTACCTCTCCCAGTTCGTCTGA

Protein Analysis

341

Amino Acids

37.49

Weight (kDa)

8.75

Isoelectric Point (pI)

30.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 36 - 337 2.2e-99 NmrA-like family
Epimerase PF01370 40 - 120 2e-08 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 44 - 185 1.2e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45970 AT3G45970 AT4G38400
fragaria_vesca FvH4_2g16110
malus_domestica MD05G1130300.v1.1 MD10G1133200.v1.1
prunus_persica Prupe.8G174500_v2.0.a1
pyrus_communis pycom05g12690 pycom10g11500
rosa_chinensis RchiOBHm_Chr6g0279781
rosa_laevigata RLG00000013120
rosa_multiflora Rmu_sc0001719.1_g000021
rosa_roxburghii Rroxscaffold_7G00188660
rosa_rugosa Rorug06G0128000
rosa_samantha Rh6AG236200 Rh6BG240400 Rh6DG234100
rosa_wichuraiana Rw6G020590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 757
AasI GACNNNNNNGTC 1 cut(s) 626
Acc36I ACCTGC 1 cut(s) 757
Acc65I GGTACC 1 cut(s) 683
AccB1I GGYRCC 1 cut(s) 683
AccB7I CCANNNNNTGG 1 cut(s) 466
AccII CGCG 1 cut(s) 359
AciI CCGC 4 cut(s) 390, 508, 524, 866
AclWI GGATC 4 cut(s) 86, 99, 459, 724
AcoI YGGCCR 4 cut(s) 181, 228, 525, 743
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 466
AfaI GTAC 3 cut(s) 293, 685, 1007
AfiI CCNNNNNNNGG 5 cut(s) 88, 313, 466, 817, 950
AgsI TTSAA 4 cut(s) 256, 724, 769, 857
AhdI GACNNNNNGTC 1 cut(s) 351
AjiI CACGTC 1 cut(s) 448
AjnI CCWGG 2 cut(s) 279, 614
AjuI GAANNNNNNNTTGG 4 cut(s) 107, 139, 663, 695
AleI CACNNNNGTG 2 cut(s) 545, 995
AluBI AGCT 3 cut(s) 252, 602, 962
AluI AGCT 3 cut(s) 252, 602, 962
Alw26I GTCTC 1 cut(s) 912
AlwI GGATC 4 cut(s) 86, 99, 459, 724
Ama87I CYCGRG 1 cut(s) 812
AoxI GGCC 7 cut(s) 165, 177, 181, 228, 258, 525, 743
ApeKI GCWGC 1 cut(s) 863
Asp718I GGTACC 1 cut(s) 683
AspLEI GCGC 3 cut(s) 103, 173, 361
AspS9I GGNCC 1 cut(s) 505
AsuC2I CCSGG 6 cut(s) 89, 206, 227, 529, 597, 830
AsuHPI GGTGA 3 cut(s) 296, 349, 650
AvaI CYCGRG 1 cut(s) 812
AvaII GGWCC 1 cut(s) 505
BamHI GGATCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 683
BanII GRGCYC 2 cut(s) 213, 945
BauI CACGAG 1 cut(s) 299
BbvI GCAGC 1 cut(s) 850
BccI CCATC 1 cut(s) 635
BceAI ACGGC 1 cut(s) 498
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BciT130I CCWGG 2 cut(s) 281, 616
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 6 cut(s) 89, 206, 227, 529, 597, 830
BcoDI GTCTC 1 cut(s) 912
BfaI CTAG 1 cut(s) 26
BfoI RGCGCY 1 cut(s) 174
BfuAI ACCTGC 1 cut(s) 757
BisI GCNGC 3 cut(s) 390, 525, 864
BlsI GCNGC 3 cut(s) 391, 526, 865
Bme1390I CCNGG 8 cut(s) 89, 206, 227, 281, 529, 597, 616, 830
Bme18I GGWCC 1 cut(s) 505
BmeRI GACNNNNNGTC 1 cut(s) 351
BmeT110I CYCGRG 1 cut(s) 812
BmgBI CACGTC 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 5 cut(s) 93, 210, 471, 685, 870
BmrFI CCNGG 8 cut(s) 89, 206, 227, 281, 529, 597, 616, 830
BmrI ACTGGG 2 cut(s) 576, 1009
BmuI ACTGGG 2 cut(s) 576, 1009
BplI GAGNNNNNCTC 1 cut(s) 995
BpuMI CCSGG 6 cut(s) 89, 206, 227, 529, 597, 830
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 912
BsaJI CCNNGG 5 cut(s) 174, 280, 501, 614, 615
BsaXI ACNNNNNCTCC 6 cut(s) 63, 93, 595, 625, 746, 776
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 313, 466, 817, 950
Bse118I RCCGGY 2 cut(s) 179, 404
Bse1I ACTGG 4 cut(s) 142, 582, 891, 1015
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 2 cut(s) 281, 616
BseDI CCNNGG 5 cut(s) 174, 280, 501, 614, 615
BseGI GGATG 4 cut(s) 14, 712, 738, 1006
BseLI CCNNNNNNNGG 5 cut(s) 88, 313, 466, 817, 950
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 915
BseNI ACTGG 4 cut(s) 142, 582, 891, 1015
BseRI GAGGAG 2 cut(s) 600, 848
BseX3I CGGCCG 1 cut(s) 525
BseXI GCAGC 1 cut(s) 850
Bsh1236I CGCG 1 cut(s) 359
Bsh1285I CGRYCG 1 cut(s) 528
BshFI GGCC 7 cut(s) 167, 179, 183, 230, 260, 527, 745
BshNI GGYRCC 1 cut(s) 683
BsiEI CGRYCG 1 cut(s) 528
BsiHKCI CYCGRG 1 cut(s) 812
BslFI GGGAC 2 cut(s) 632, 812
BslI CCNNNNNNNGG 5 cut(s) 88, 313, 466, 817, 950
BsmAI GTCTC 1 cut(s) 912
BsmFI GGGAC 2 cut(s) 632, 812
BsnI GGCC 7 cut(s) 167, 179, 183, 230, 260, 527, 745
Bso31I GGTCTC 1 cut(s) 912
BsoBI CYCGRG 1 cut(s) 812
Bsp1286I GDGCHC 2 cut(s) 213, 945
Bsp143I GATC 7 cut(s) 91, 286, 370, 381, 451, 729, 936
BspACI CCGC 4 cut(s) 390, 508, 524, 866
BspANI GGCC 7 cut(s) 167, 179, 183, 230, 260, 527, 745
BspCNI CTCAG 1 cut(s) 914
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 5 cut(s) 93, 210, 471, 685, 870
BspMI ACCTGC 1 cut(s) 757
BspPI GGATC 4 cut(s) 86, 99, 459, 724
BspT107I GGYRCC 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 912
BsrDI GCAATG 1 cut(s) 415
BsrFI RCCGGY 2 cut(s) 179, 404
BsrI ACTGG 4 cut(s) 142, 582, 891, 1015
BssAI RCCGGY 2 cut(s) 179, 404
BssECI CCNNGG 5 cut(s) 174, 280, 501, 614, 615
BssMI GATC 7 cut(s) 91, 286, 370, 381, 451, 729, 936
BssNI GRCGYC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 2 cut(s) 174, 501
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 2 cut(s) 281, 616
Bst4CI ACNGT 2 cut(s) 349, 997
Bst6I CTCTTC 2 cut(s) 128, 666
BstACI GRCGYC 1 cut(s) 267
BstC8I GCNNGC 4 cut(s) 169, 181, 406, 474
BstDEI CTNAG 1 cut(s) 901
BstENI CCTNNNNNAGG 2 cut(s) 311, 815
BstF5I GGATG 4 cut(s) 14, 712, 738, 1006
BstFNI CGCG 1 cut(s) 359
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 3 cut(s) 103, 173, 361
BstKTI GATC 7 cut(s) 94, 289, 373, 384, 454, 732, 939
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 7 cut(s) 91, 286, 370, 381, 451, 729, 936
BstMCI CGRYCG 1 cut(s) 528
BstMWI GCNNNNNNNGC 3 cut(s) 365, 482, 497
BstNI CCWGG 2 cut(s) 281, 616
BstSCI CCNGG 8 cut(s) 87, 204, 225, 279, 527, 595, 614, 828
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 1 cut(s) 850
BstX2I RGATCY 2 cut(s) 91, 729
BstYI RGATCY 2 cut(s) 91, 729
BstZI CGGCCG 1 cut(s) 525
BsuRI GGCC 7 cut(s) 167, 179, 183, 230, 260, 527, 745
BtgZI GCGATG 1 cut(s) 370
BtrI CACGTC 1 cut(s) 448
BtsCI GGATG 4 cut(s) 14, 712, 738, 1006
BtsIMutI CAGTG 1 cut(s) 135
BveI ACCTGC 1 cut(s) 757
Cac8I GCNNGC 4 cut(s) 169, 181, 406, 474
CfoI GCGC 3 cut(s) 103, 173, 361
Cfr10I RCCGGY 2 cut(s) 179, 404
Cfr13I GGNCC 1 cut(s) 505
CseI GACGC 1 cut(s) 256
Csp6I GTAC 3 cut(s) 292, 684, 1006
CviQI GTAC 3 cut(s) 292, 684, 1006
DdeI CTNAG 1 cut(s) 901
DpnI GATC 7 cut(s) 93, 288, 372, 383, 453, 731, 938
DpnII GATC 7 cut(s) 91, 286, 370, 381, 451, 729, 936
DraIII CACNNNGTG 1 cut(s) 466
DrdI GACNNNNNNGTC 1 cut(s) 626
DriI GACNNNNNGTC 1 cut(s) 351
DseDI GACNNNNNNGTC 1 cut(s) 626
EaeI YGGCCR 4 cut(s) 181, 228, 525, 743
EagI CGGCCG 1 cut(s) 525
Eam1104I CTCTTC 2 cut(s) 128, 666
Eam1105I GACNNNNNGTC 1 cut(s) 351
EarI CTCTTC 2 cut(s) 128, 666
EclXI CGGCCG 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 174, 501
Eco147I AGGCCT 1 cut(s) 260
Eco24I GRGCYC 2 cut(s) 213, 945
Eco31I GGTCTC 1 cut(s) 912
Eco47I GGWCC 1 cut(s) 505
Eco52I CGGCCG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 812
EcoNI CCTNNNNNAGG 2 cut(s) 311, 815
EcoRII CCWGG 2 cut(s) 279, 614
EcoT14I CCWWGG 2 cut(s) 174, 501
EcoT38I GRGCYC 2 cut(s) 213, 945
ErhI CCWWGG 2 cut(s) 174, 501
FaiI YATR 4 cut(s) 158, 546, 690, 825
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FaqI GGGAC 2 cut(s) 632, 812
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 3 cut(s) 390, 525, 864
FokI GGATG 3 cut(s) 719, 725, 1013
FriOI GRGCYC 2 cut(s) 213, 945
FseI GGCCGGCC 1 cut(s) 183
Fsp4HI GCNGC 3 cut(s) 390, 525, 864
FspBI CTAG 1 cut(s) 26
GlaI GCGC 3 cut(s) 102, 172, 360
GluI GCNGC 3 cut(s) 390, 525, 864
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 7 cut(s) 167, 179, 183, 230, 260, 527, 745
HgaI GACGC 1 cut(s) 256
HhaI GCGC 3 cut(s) 103, 173, 361
Hin1I GRCGYC 1 cut(s) 267
Hin6I GCGC 3 cut(s) 101, 171, 359
HinP1I GCGC 3 cut(s) 101, 171, 359
HindIII AAGCTT 1 cut(s) 960
HinfI GANTC 4 cut(s) 78, 118, 456, 532
HphI GGTGA 3 cut(s) 296, 349, 650
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 5 cut(s) 71, 131, 433, 588, 1025
Hpy188III TCNNGA 3 cut(s) 415, 814, 857
Hpy8I GTNNAC 1 cut(s) 460
Hpy99I CGWCG 4 cut(s) 75, 78, 349, 449
HpyAV CCTTC 6 cut(s) 202, 307, 438, 769, 775, 811
HpyCH4III ACNGT 2 cut(s) 349, 997
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 482, 497
HpyF3I CTNAG 1 cut(s) 901
HpySE526I ACGT 1 cut(s) 447
Hsp92I GRCGYC 1 cut(s) 267
HspAI GCGC 3 cut(s) 101, 171, 359
KpnI GGTACC 1 cut(s) 687
KroI GCCGGC 2 cut(s) 179, 404
KroNI GCCGGC 2 cut(s) 181, 406
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 7 cut(s) 91, 286, 370, 381, 451, 729, 936
LmnI GCTCC 5 cut(s) 208, 469, 599, 613, 835
Lsp1109I GCAGC 1 cut(s) 850
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 3 cut(s) 353, 569, 896
MalI GATC 7 cut(s) 93, 288, 372, 383, 453, 731, 938
MboI GATC 7 cut(s) 91, 286, 370, 381, 451, 729, 936
MboII GAAGA 4 cut(s) 115, 683, 805, 808
MflI RGATCY 2 cut(s) 91, 729
MhlI GDGCHC 2 cut(s) 213, 945
MluCI AATT 1 cut(s) 271
MlyI GAGTC 2 cut(s) 72, 465
MmeI TCCRAC 2 cut(s) 94, 246
MroNI GCCGGC 2 cut(s) 179, 404
MseI TTAA 4 cut(s) 321, 396, 663, 696
MslI CAYNNNNRTG 2 cut(s) 545, 995
MspR9I CCNGG 8 cut(s) 89, 206, 227, 281, 529, 597, 616, 830
MvaI CCWGG 2 cut(s) 281, 616
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 3 cut(s) 365, 482, 497
NaeI GCCGGC 2 cut(s) 181, 406
NciI CCSGG 6 cut(s) 89, 206, 227, 529, 597, 830
NdeII GATC 7 cut(s) 91, 286, 370, 381, 451, 729, 936
NgoMIV GCCGGC 2 cut(s) 179, 404
NlaIV GGNNCC 5 cut(s) 93, 210, 471, 685, 870
NmuCI GTSAC 2 cut(s) 353, 896
OliI CACNNNNGTG 2 cut(s) 545, 995
PaeR7I CTCGAG 1 cut(s) 812
PaqCI CACCTGC 1 cut(s) 757
PasI CCCWGGG 1 cut(s) 615
PceI AGGCCT 1 cut(s) 260
PdiI GCCGGC 2 cut(s) 181, 406
PfeI GAWTC 2 cut(s) 118, 532
PflFI GACNNNGTC 1 cut(s) 623
PflMI CCANNNNNTGG 1 cut(s) 466
PfoI TCCNGGA 3 cut(s) 87, 204, 828
PkrI GCNGC 3 cut(s) 391, 526, 865
PleI GAGTC 2 cut(s) 72, 464
PpsI GAGTC 2 cut(s) 72, 464
Psp6I CCWGG 2 cut(s) 279, 614
PspGI CCWGG 2 cut(s) 279, 614
PspN4I GGNNCC 5 cut(s) 93, 210, 471, 685, 870
PspPI GGNCC 1 cut(s) 505
PsuI RGATCY 2 cut(s) 91, 729
PsyI GACNNNGTC 1 cut(s) 623
RigI GGCCGGCC 1 cut(s) 183
RsaI GTAC 3 cut(s) 293, 685, 1007
RsaNI GTAC 3 cut(s) 292, 684, 1006
RseI CAYNNNNRTG 2 cut(s) 545, 995
SaqAI TTAA 4 cut(s) 321, 396, 663, 696
SatI GCNGC 3 cut(s) 390, 525, 864
Sau3AI GATC 7 cut(s) 91, 286, 370, 381, 451, 729, 936
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 2 cut(s) 72, 465
ScrFI CCNGG 8 cut(s) 89, 206, 227, 281, 529, 597, 616, 830
SduI GDGCHC 2 cut(s) 213, 945
Sfr274I CTCGAG 1 cut(s) 812
SinI GGWCC 1 cut(s) 505
SlaI CTCGAG 1 cut(s) 812
SmiMI CAYNNNNRTG 2 cut(s) 545, 995
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
Sse9I AATT 1 cut(s) 271
SseBI AGGCCT 1 cut(s) 260
SsiI CCGC 4 cut(s) 390, 508, 524, 866
SspMI CTAG 1 cut(s) 26
StuI AGGCCT 1 cut(s) 260
StyD4I CCNGG 8 cut(s) 87, 204, 225, 279, 527, 595, 614, 828
StyI CCWWGG 2 cut(s) 174, 501
TaaI ACNGT 2 cut(s) 349, 997
TaiI ACGT 1 cut(s) 450
TaqI TCGA 7 cut(s) 162, 246, 330, 344, 454, 813, 939
TaqII GACCGA 1 cut(s) 884
TasI AATT 1 cut(s) 271
TauI GCSGC 2 cut(s) 392, 527
TfiI GAWTC 2 cut(s) 118, 532
Tru1I TTAA 4 cut(s) 321, 396, 663, 696
Tru9I TTAA 4 cut(s) 321, 396, 663, 696
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 2 cut(s) 353, 896
TseI GCWGC 1 cut(s) 863
Tsp45I GTSAC 2 cut(s) 353, 896
TspDTI ATGAA 4 cut(s) 27, 115, 145, 723
TspRI CASTG 1 cut(s) 142
Tth111I GACNNNGTC 1 cut(s) 623
Van91I CCANNNNNTGG 1 cut(s) 466
VpaK11BI GGWCC 1 cut(s) 505
XagI CCTNNNNNAGG 2 cut(s) 311, 815
XcmI CCANNNNNNNNNTGG 1 cut(s) 700
XhoI CTCGAG 1 cut(s) 812
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.