Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0009359

Physical Location & Seq

Genomic Coordinates Forward (+)

20459601 .. 20463119

Gene Structure 3519 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g17250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGCCCAAACCAAAGTAAGCATATGCCCGACTCCCAACCGATCCTACCCATCTTCTTCATCCAACAGGTCTCTCTCTCTCTCCTTTCTCTCTCTAGCTCTCTCTGAAGAACCTGCAGCTGAGAGCACGATGCAGGCCGTCTCGAAGCGCGTTGGGCGCCAGTATCTGACCCAATCATCTTCCATCTCGTCGCTCAAGTCAATCTATCCCCTTTCCGATCACTATTATGGAGCTGATCGTCCAAAATACGGATCTACCCTCGCCACCAAGGGAGTGGGGCACCTTGTGCGCAAGGGCACAGGTGGAAGATCATCTGTTAGTGGCATTGTTGCTGCAGTCTTTGGATCTACGGGGTTCCTTGGGCGTTATCTTGTGCAACAACTTGCCAAAATGGGAAGTCAGGTGTTAGTTCCTTTCCGTGGTTCTGAAGACTCCCATCGTCACCTCAAGTTAATGGGGGATTTGGGACAGATTGTACCAATGAAATACAATCCGCGAGATGAAGATTCAATTAAGGCAGTCATGGCAAAAGCAAACGTGGTTATCAATCTCATAGGGAGGGATTATGAGACAAGAAACTTTAGCTTTGAGGAAGTGAATCATTCCATGGCGCAACAACTGGCAACGATTTCCAAAGAACATGGGGGTATCATGAGATTTATTCAAGTTTCTTGCTTGGGGGCATCTTCCTCGTCTCCCTCCAGATTCCTAAGGACTAAAGCTGCTGCAGAGGAAGCTGTTTTAAGTGAATTGCCTGAGGCCACCATTTTGAGACCTGCTGTGATGGTTGGTACTGAGGATCGGATCTTGAATCGCTGGGCCTTTTTTGCAAAAAAATATGGCTTTCTTCCACTTGTTGAGGATCGATCTACTAAATTCCAACCCGTGTATGTTGTTGATGTTGCTGGGGCAATCGTGGCAGCCTTGAAAGATGATGGTACCAGCATGGGAAAAATTTATGAACTTGGTGGGCCTGAGGTCTTTACAATGCATCAATTGGCAGAGCTTATGTTTGATACAATCCGTGAATGGCCTCGCTACGTGAAGGTTCCCCTCCCAATTGCAAAGGCAATAGGTGCTCCACGAGAAATATTGCTTAACAAAGTTCCGTTTCCATTACCTAATCCTGAAATCTTCAACCGAGATCAAATCCTTGCCCAAGCTACAGATACACTCGTGTCAGAAAATGCTTTGACATTCAGTGATCTTGGACTTGTGCCCCATAAGCTGAAGGGTTACCCTATTGAGCTTCTTATCCAATTTCGCAAGGGTGGCCCAAATTACGGTTCTACAGTCAGTGAAAGAGTGTCTCCAGATGCTTGGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003954 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005746 GO:0005747 GO:0006732 GO:0006733 GO:0006743 GO:0006744 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009628 GO:0009651 GO:0009987 GO:0016020 GO:0016491 GO:0016651 GO:0019866 GO:0030964 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0042180 GO:0042181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045271 GO:0050896 GO:0051186 GO:0051188 GO:0055114 GO:0070469 GO:0071704 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:1901004 GO:1901006 GO:1901576 GO:1901661 GO:1901663 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016

Pfam Domains

Protein Families

Epimerase NAD_binding_10 NmrA

Protein Analysis

443

Amino Acids

48.41

Weight (kDa)

9.31

Isoelectric Point (pI)

44.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	112 - 323	6e-11	NAD dependent epimerase/dehydratase family
NmrA	PF05368	112 - 363	1.8e-09	NmrA-like family
NAD_binding_10	PF13460	115 - 262	1.9e-08	NAD(P)H-binding
RmlD_sub_bind	PF04321	238 - 345	3.4e-06	RmlD substrate binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20360
fragaria_vesca	FvH4_2g20370 FvH4_2g20370 FvH4_2g20370
malus_domestica	MD05G1187700.v1.1 MD10G1175400.v1.1
prunus_persica	Prupe.8G214700_v2.0.a1 Prupe.8G214700_v2.0.a1 Prupe.8G214700_v2.0.a1
pyrus_communis	pycom05g17250 pycom10g15110 pycom10g15120
rosa_chinensis	RchiOBHm_Chr6g0286481
rosa_laevigata	RLG00000012568
rosa_multiflora	Rmu_sc0004816.1_g000013 Rmu_sc0005089.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182300
rosa_rugosa	Rorug06G0180100
rosa_samantha	Rh6AG292300 Rh6CG296600 Rh6DG288400
rosa_wichuraiana	Rw6G025210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	290
Acc36I	ACCTGC	2 cut(s)	121, 784
Acc65I	GGTACC	1 cut(s)	938
AccB1I	GGYRCC	3 cut(s)	156, 279, 938
AccII	CGCG	2 cut(s)	150, 496
AciI	CCGC	1 cut(s)	494
AclWI	GGATC	6 cut(s)	36, 259, 352, 807, 812, 870
AcoI	YGGCCR	1 cut(s)	1322
AcsI	RAATTY	2 cut(s)	875, 954
AcuI	CTGAAG	3 cut(s)	126, 447, 1250
AcyI	GRCGYC	1 cut(s)	157
AdeI	CACNNNGTG	1 cut(s)	286
AfaI	GTAC	3 cut(s)	477, 793, 940
AfiI	CCNNNNNNNGG	3 cut(s)	248, 419, 1283
AgsI	TTSAA	5 cut(s)	510, 665, 811, 928, 1138
AjuI	GAANNNNNNNTTGG	2 cut(s)	1270, 1302
Alw21I	GWGCWC	2 cut(s)	128, 1081
Alw26I	GTCTC	6 cut(s)	75, 145, 563, 699, 766, 1314
AlwI	GGATC	6 cut(s)	36, 259, 352, 807, 812, 870
AlwNI	CAGNNNCTG	1 cut(s)	166
AoxI	GGCC	8 cut(s)	3, 135, 759, 819, 971, 1031, 1273, 1322
ApeKI	GCWGC	5 cut(s)	116, 332, 722, 725, 920
ApoI	RAATTY	2 cut(s)	875, 954
Asp718I	GGTACC	1 cut(s)	938
AspLEI	GCGC	4 cut(s)	150, 159, 291, 613
AspS9I	GGNCC	4 cut(s)	4, 819, 971, 1274
AsuHPI	GGTGA	1 cut(s)	434
AxyI	CCTNAGG	3 cut(s)	710, 756, 975
BaeGI	GKGCMC	3 cut(s)	282, 299, 1221
BaeI	ACNNNNGTAYC	2 cut(s)	1161, 1194
BalI	TGGCCA	1 cut(s)	1324
BanI	GGYRCC	3 cut(s)	156, 279, 938
BauI	CACGAG	2 cut(s)	1083, 1175
BbsI	GAAGAC	1 cut(s)	435
Bbv12I	GWGCWC	2 cut(s)	128, 1081
BbvI	GCAGC	5 cut(s)	128, 319, 709, 712, 932
BccI	CCATC	5 cut(s)	58, 191, 444, 778, 929
BceAI	ACGGC	1 cut(s)	122
BcgI	CGANNNNNNTGC	2 cut(s)	672, 706
BcoDI	GTCTC	6 cut(s)	75, 145, 563, 699, 766, 1314
BfaI	CTAG	1 cut(s)	95
BfmI	CTRYAG	5 cut(s)	114, 333, 726, 1164, 1290
BfoI	RGCGCY	1 cut(s)	160
BfuAI	ACCTGC	2 cut(s)	121, 784
BisI	GCNGC	5 cut(s)	117, 333, 723, 726, 921
BlsI	GCNGC	5 cut(s)	118, 334, 724, 727, 922
BmgT120I	GGNCC	4 cut(s)	4, 819, 971, 1274
BmiI	GGNNCC	5 cut(s)	158, 281, 356, 940, 1050
BmsI	GCATC	4 cut(s)	120, 692, 1000, 1306
BpiI	GAAGAC	1 cut(s)	435
BpmI	CTGGAG	2 cut(s)	685, 1296
BpuEI	CTTGAG	2 cut(s)	179, 431
Bsa29I	ATCGAT	1 cut(s)	865
BsaAI	YACGTR	1 cut(s)	1042
BsaHI	GRCGYC	1 cut(s)	157
BsaI	GGTCTC	2 cut(s)	75, 766
BsaJI	CCNNGG	4 cut(s)	267, 358, 418, 606
Bsc4I	CCNNNNNNNGG	3 cut(s)	248, 419, 1283
Bse1I	ACTGG	2 cut(s)	160, 624
Bse21I	CCTNAGG	3 cut(s)	710, 756, 975
BseCI	ATCGAT	1 cut(s)	865
BseDI	CCNNGG	4 cut(s)	267, 358, 418, 606
BseGI	GGATG	1 cut(s)	59
BseLI	CCNNNNNNNGG	3 cut(s)	248, 419, 1283
BseMII	CTCAG	4 cut(s)	111, 747, 786, 966
BseNI	ACTGG	2 cut(s)	160, 624
BseSI	GKGCMC	3 cut(s)	282, 299, 1221
BseXI	GCAGC	5 cut(s)	128, 319, 709, 712, 932
BseYI	CCCAGC	2 cut(s)	816, 905
Bsh1236I	CGCG	2 cut(s)	150, 496
BshFI	GGCC	8 cut(s)	5, 137, 761, 821, 973, 1033, 1275, 1324
BshNI	GGYRCC	3 cut(s)	156, 279, 938
BshVI	ATCGAT	1 cut(s)	865
BsiHKAI	GWGCWC	2 cut(s)	128, 1081
BslFI	GGGAC	1 cut(s)	480
BslI	CCNNNNNNNGG	3 cut(s)	248, 419, 1283
BsmAI	GTCTC	6 cut(s)	75, 145, 563, 699, 766, 1314
BsmBI	CGTCTC	2 cut(s)	145, 699
BsmFI	GGGAC	1 cut(s)	480
BsnI	GGCC	8 cut(s)	5, 137, 761, 821, 973, 1033, 1275, 1324
Bso31I	GGTCTC	2 cut(s)	75, 766
Bsp1286I	GDGCHC	5 cut(s)	128, 282, 299, 1081, 1221
Bsp19I	CCATGG	1 cut(s)	606
BspACI	CCGC	1 cut(s)	494
BspANI	GGCC	8 cut(s)	5, 137, 761, 821, 973, 1033, 1275, 1324
BspCNI	CTCAG	4 cut(s)	112, 748, 787, 967
BspDI	ATCGAT	1 cut(s)	865
BspFNI	CGCG	2 cut(s)	150, 496
BspHI	TCATGA	1 cut(s)	651
BspLI	GGNNCC	5 cut(s)	158, 281, 356, 940, 1050
BspMAI	CTGCAG	3 cut(s)	118, 337, 730
BspMI	ACCTGC	2 cut(s)	121, 784
BspPI	GGATC	6 cut(s)	36, 259, 352, 807, 812, 870
BspT107I	GGYRCC	3 cut(s)	156, 279, 938
BspTNI	GGTCTC	2 cut(s)	75, 766
BsrI	ACTGG	2 cut(s)	160, 624
BssECI	CCNNGG	4 cut(s)	267, 358, 418, 606
BssNI	GRCGYC	1 cut(s)	157
BssSI	CACGAG	2 cut(s)	1083, 1175
BssT1I	CCWWGG	3 cut(s)	267, 358, 606
Bst2BI	CACGAG	2 cut(s)	1083, 1175
Bst4CI	ACNGT	2 cut(s)	1286, 1294
BstACI	GRCGYC	1 cut(s)	157
BstAPI	GCANNNNNTGC	2 cut(s)	286, 1076
BstBAI	YACGTR	1 cut(s)	1042
BstC8I	GCNNGC	1 cut(s)	135
BstDEI	CTNAG	5 cut(s)	120, 710, 756, 795, 975
BstDSI	CCRYGG	2 cut(s)	418, 606
BstEII	GGTNACC	1 cut(s)	1235
BstF5I	GGATG	1 cut(s)	59
BstFNI	CGCG	2 cut(s)	150, 496
BstH2I	RGCGCY	1 cut(s)	160
BstHHI	GCGC	4 cut(s)	150, 159, 291, 613
BstMAI	GTCTC	6 cut(s)	75, 145, 563, 699, 766, 1314
BstPI	GGTNACC	1 cut(s)	1235
BstSFI	CTRYAG	5 cut(s)	114, 333, 726, 1164, 1290
BstSLI	GKGCMC	3 cut(s)	282, 299, 1221
BstUI	CGCG	2 cut(s)	150, 496
BstV1I	GCAGC	5 cut(s)	128, 319, 709, 712, 932
BstV2I	GAAGAC	1 cut(s)	435
BstX2I	RGATCY	3 cut(s)	251, 344, 804
BstXI	CCANNNNNNTGG	2 cut(s)	274, 1320
BstYI	RGATCY	3 cut(s)	251, 344, 804
Bsu15I	ATCGAT	1 cut(s)	865
Bsu36I	CCTNAGG	3 cut(s)	710, 756, 975
BsuRI	GGCC	8 cut(s)	5, 137, 761, 821, 973, 1033, 1275, 1324
BsuTUI	ATCGAT	1 cut(s)	865
BtgI	CCRYGG	2 cut(s)	418, 606
BtsCI	GGATG	1 cut(s)	59
BtsIMutI	CAGTG	2 cut(s)	1207, 1303
BveI	ACCTGC	2 cut(s)	121, 784
Cac8I	GCNNGC	1 cut(s)	135
CaiI	CAGNNNCTG	1 cut(s)	166
CciI	TCATGA	1 cut(s)	651
CfoI	GCGC	4 cut(s)	150, 159, 291, 613
Cfr13I	GGNCC	4 cut(s)	4, 819, 971, 1274
ClaI	ATCGAT	1 cut(s)	865
Csp6I	GTAC	3 cut(s)	476, 792, 939
CviAII	CATG	6 cut(s)	523, 607, 641, 652, 946, 1326
CviQI	GTAC	3 cut(s)	476, 792, 939
DdeI	CTNAG	5 cut(s)	120, 710, 756, 795, 975
DinI	GGCGCC	1 cut(s)	158
DraIII	CACNNNGTG	1 cut(s)	286
EaeI	YGGCCR	1 cut(s)	1322
Eco130I	CCWWGG	3 cut(s)	267, 358, 606
Eco31I	GGTCTC	2 cut(s)	75, 766
Eco57I	CTGAAG	3 cut(s)	126, 447, 1250
Eco81I	CCTNAGG	3 cut(s)	710, 756, 975
Eco91I	GGTNACC	1 cut(s)	1235
EcoO65I	GGTNACC	1 cut(s)	1235
EcoT14I	CCWWGG	3 cut(s)	267, 358, 606
EcoT22I	ATGCAT	1 cut(s)	993
EgeI	GGCGCC	1 cut(s)	158
EheI	GGCGCC	1 cut(s)	158
ErhI	CCWWGG	3 cut(s)	267, 358, 606
Esp3I	CGTCTC	2 cut(s)	145, 699
FaeI	CATG	6 cut(s)	526, 610, 644, 655, 949, 1329
FaqI	GGGAC	1 cut(s)	480
FatI	CATG	6 cut(s)	522, 606, 640, 651, 945, 1325
FauNDI	CATATG	1 cut(s)	23
Fnu4HI	GCNGC	5 cut(s)	117, 333, 723, 726, 921
FokI	GGATG	1 cut(s)	46
Fsp4HI	GCNGC	5 cut(s)	117, 333, 723, 726, 921
FspBI	CTAG	1 cut(s)	95
FspI	TGCGCA	1 cut(s)	290
GlaI	GCGC	4 cut(s)	149, 158, 290, 612
GluI	GCNGC	5 cut(s)	117, 333, 723, 726, 921
GsaI	CCCAGC	2 cut(s)	820, 909
GsuI	CTGGAG	2 cut(s)	685, 1296
HaeII	RGCGCY	1 cut(s)	160
HaeIII	GGCC	8 cut(s)	5, 137, 761, 821, 973, 1033, 1275, 1324
HhaI	GCGC	4 cut(s)	150, 159, 291, 613
Hin1I	GRCGYC	1 cut(s)	157
Hin1II	CATG	6 cut(s)	526, 610, 644, 655, 949, 1329
Hin6I	GCGC	4 cut(s)	148, 157, 289, 611
HinP1I	GCGC	4 cut(s)	148, 157, 289, 611
HinfI	GANTC	6 cut(s)	31, 431, 506, 598, 705, 811
HphI	GGTGA	1 cut(s)	434
Hpy188I	TCNGA	6 cut(s)	106, 168, 217, 427, 804, 1183
Hpy188III	TCNNGA	6 cut(s)	142, 652, 702, 808, 1127, 1313
Hpy99I	CGWCG	1 cut(s)	193
HpyAV	CCTTC	2 cut(s)	1039, 1225
HpyCH4III	ACNGT	2 cut(s)	1286, 1294
HpyCH4IV	ACGT	2 cut(s)	537, 1041
HpyCH4V	TGCA	8 cut(s)	116, 133, 335, 376, 728, 830, 991, 1064
HpyF3I	CTNAG	5 cut(s)	120, 710, 756, 795, 975
HpySE526I	ACGT	2 cut(s)	537, 1041
Hsp92I	GRCGYC	1 cut(s)	157
Hsp92II	CATG	6 cut(s)	526, 610, 644, 655, 949, 1329
HspAI	GCGC	4 cut(s)	148, 157, 289, 611
KasI	GGCGCC	1 cut(s)	156
KpnI	GGTACC	1 cut(s)	942
LmnI	GCTCC	2 cut(s)	230, 1084
Lsp1109I	GCAGC	5 cut(s)	128, 319, 709, 712, 932
LweI	GCATC	4 cut(s)	120, 692, 1000, 1306
MaeI	CTAG	1 cut(s)	95
MaeII	ACGT	2 cut(s)	537, 1041
MaeIII	GTNAC	2 cut(s)	440, 1235
MfeI	CAATTG	2 cut(s)	995, 1059
MflI	RGATCY	3 cut(s)	251, 344, 804
MhlI	GDGCHC	5 cut(s)	128, 282, 299, 1081, 1221
MlsI	TGGCCA	1 cut(s)	1324
MluCI	AATT	8 cut(s)	510, 749, 875, 954, 995, 1059, 1259, 1279
MluNI	TGGCCA	1 cut(s)	1324
Mly113I	GGCGCC	1 cut(s)	157
MlyI	GAGTC	2 cut(s)	25, 425
MmeI	TCCRAC	2 cut(s)	87, 904
Mox20I	TGGCCA	1 cut(s)	1324
Mph1103I	ATGCAT	1 cut(s)	993
MscI	TGGCCA	1 cut(s)	1324
MseI	TTAA	4 cut(s)	452, 513, 743, 1098
MslI	CAYNNNNRTG	1 cut(s)	225
Msp20I	TGGCCA	1 cut(s)	1324
MspA1I	CMGCKG	1 cut(s)	119
MunI	CAATTG	2 cut(s)	995, 1059
MvnI	CGCG	2 cut(s)	150, 496
NarI	GGCGCC	1 cut(s)	157
NcoI	CCATGG	1 cut(s)	606
NdeI	CATATG	1 cut(s)	23
NlaIII	CATG	6 cut(s)	526, 610, 644, 655, 949, 1329
NlaIV	GGNNCC	5 cut(s)	158, 281, 356, 940, 1050
NmuCI	GTSAC	1 cut(s)	440
NsbI	TGCGCA	1 cut(s)	290
NsiI	ATGCAT	1 cut(s)	993
PagI	TCATGA	1 cut(s)	651
PfeI	GAWTC	4 cut(s)	506, 598, 705, 811
PkrI	GCNGC	5 cut(s)	118, 334, 724, 727, 922
PleI	GAGTC	2 cut(s)	25, 425
PluTI	GGCGCC	1 cut(s)	160
PpsI	GAGTC	2 cut(s)	25, 425
Ppu21I	YACGTR	1 cut(s)	1042
PspEI	GGTNACC	1 cut(s)	1235
PspFI	CCCAGC	2 cut(s)	816, 905
PspN4I	GGNNCC	5 cut(s)	158, 281, 356, 940, 1050
PspPI	GGNCC	4 cut(s)	4, 819, 971, 1274
PstI	CTGCAG	3 cut(s)	118, 337, 730
PstNI	CAGNNNCTG	1 cut(s)	166
PsuI	RGATCY	3 cut(s)	251, 344, 804
PvuII	CAGCTG	1 cut(s)	119
RsaI	GTAC	3 cut(s)	477, 793, 940
RsaNI	GTAC	3 cut(s)	476, 792, 939
RseI	CAYNNNNRTG	1 cut(s)	225
SaqAI	TTAA	4 cut(s)	452, 513, 743, 1098
SatI	GCNGC	5 cut(s)	117, 333, 723, 726, 921
Sau96I	GGNCC	4 cut(s)	4, 819, 971, 1274
SchI	GAGTC	2 cut(s)	25, 425
SduI	GDGCHC	5 cut(s)	128, 282, 299, 1081, 1221
SfaNI	GCATC	4 cut(s)	120, 692, 1000, 1306
SfcI	CTRYAG	5 cut(s)	114, 333, 726, 1164, 1290
SfoI	GGCGCC	1 cut(s)	158
SmiMI	CAYNNNNRTG	1 cut(s)	225
SmlI	CTYRAG	2 cut(s)	194, 446
SmoI	CTYRAG	2 cut(s)	194, 446
Sse9I	AATT	8 cut(s)	510, 749, 875, 954, 995, 1059, 1259, 1279
SsiI	CCGC	1 cut(s)	494
SspDI	GGCGCC	1 cut(s)	156
SspI	AATATT	1 cut(s)	1092
SspMI	CTAG	1 cut(s)	95
StyI	CCWWGG	3 cut(s)	267, 358, 606
TaaI	ACNGT	2 cut(s)	1286, 1294
TaiI	ACGT	2 cut(s)	540, 1044
TaqI	TCGA	2 cut(s)	143, 865
TasI	AATT	8 cut(s)	510, 749, 875, 954, 995, 1059, 1259, 1279
TfiI	GAWTC	4 cut(s)	506, 598, 705, 811
Tru1I	TTAA	4 cut(s)	452, 513, 743, 1098
Tru9I	TTAA	4 cut(s)	452, 513, 743, 1098
TscAI	CASTG	2 cut(s)	1207, 1303
TseFI	GTSAC	1 cut(s)	440
TseI	GCWGC	5 cut(s)	116, 332, 722, 725, 920
Tsp45I	GTSAC	1 cut(s)	440
TspDTI	ATGAA	4 cut(s)	48, 497, 516, 975
TspGWI	ACGGA	4 cut(s)	264, 407, 1013, 1098
TspRI	CASTG	2 cut(s)	1207, 1303
XapI	RAATTY	2 cut(s)	875, 954
XcmI	CCANNNNNNNNNTGG	1 cut(s)	271
XspI	CTAG	1 cut(s)	95
Zsp2I	ATGCAT	1 cut(s)	993

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.