pycom05g18740

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012096
Physical Location & Seq
Forward (+)
21573135 .. 21576167
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGCTTCGTGAAAGCAATGTCTTGAAATTTGTCAAAGATTCCAGTGTCATGAGGCCTGGATATTATATAGGGATCGATCCTCGTAAAAAACTTGTGATTTTAGGAATCCGTGGTACTCATACTGTATATGACCTTATAACTGATGTTGTTTCTTCAAGCGATGATGAAGTCACGTTTGAGGGTTATTCAACGCACTTTGGCACTGCAGAAGCTGCTCGCTGGTTTCTTAGTCACGAGATGGGAAATATAAGGAAGTGCTTGGAGAAATATGAGGGATTTAAGTTAAGGCTGGTTGGTCATTCTCTCGGAGGTTCTACAGCTGCTTTACTTGCAATAATGCTCCGTAAAAAGTCAGTTAAGGAGCTGGGGTTCAACCCAGAAATTGTCTCTGCCGTGGGGTATGCAACACCGCCTTGTGTCTCCAAAGAACTTGCCGAAAACTGCTCCAGTTACGTCACAACTGTTGTGATGCAGGATGATATTATACCTAGGCTAAGTCCAGCATCTCTAACGAGGTTGAGGAGTGAAATTCTTAAAACTGATTGGATGAGTGTGATTGAGAAGGAAGATTGGAAAAGTGTCATAGATTTGGTTACAAATGCAAAGCAGGTTGTGTCTTCGGTGCAAGATGTTGCTCGGAAGCTTTCTGATTATGCAAAGTTTGGGAGCAGTAAACATTCTTCTGATGGTCCCATCATAAAGAAAATTCCTACTGCTCCCGGGGTCCCTTTAAATGACAGAGAAGTAACAGATAATGCTGGTGCTATTAAAAGTGGAGGAGCTGCCTGTAAAGTGCCTGAGGAATTATTTGTACCAGGTACCGTCTACTGCCTAAAGAGGAATGTGGATGCTCGTACCGGCAGCAGAGGCAGCAGAAGGGAGTATTTTACACTTTGGAAGAGGCATCCGGGTGAGCATTTTCAGAGGATTGTGCTGTCAAGCAACATAATTACTGACCACAAGTGTGTGAGCCATTACTATGCATTAAGAGATGTGCTCAAAGGTTTGCCTGCCTCCGATGATGAAGACAAATTTTCTTAA

Protein Analysis

347

Amino Acids

38.38

Weight (kDa)

8.84

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 34 - 168 7.6e-28 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012096)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42450
fragaria_vesca FvH4_2g21920 FvH4_2g21920
malus_domestica MD05G1200600.v1.1 MD10G1187200.v1.1
prunus_persica Prupe.8G227200_v2.0.a1
pyrus_communis pycom05g18740
rosa_chinensis RchiOBHm_Chr6g0288821
rosa_laevigata RLG00000012365
rosa_multiflora Rmu_sc0005314.1_g000019
rosa_roxburghii Rroxscaffold_7G00179870
rosa_rugosa Rorug06G0198300 Rorug06G0198300 Rorug06G0198300
rosa_samantha Rh6AG309600 Rh6BG315500 Rh6CG323000 Rh6DG308700
rosa_wichuraiana Rw6G026760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 598
Acc65I GGTACC 1 cut(s) 818
AccB1I GGYRCC 1 cut(s) 818
AccI GTMKAC 1 cut(s) 825
AciI CCGC 1 cut(s) 410
AclWI GGATC 2 cut(s) 71, 80
AcsI RAATTY 4 cut(s) 26, 528, 705, 1031
AfaI GTAC 4 cut(s) 115, 813, 820, 856
AgsI TTSAA 4 cut(s) 25, 156, 189, 373
AjnI CCWGG 2 cut(s) 55, 814
AleI CACNNNNGTG 1 cut(s) 963
AloI GAACNNNNNNTCC 2 cut(s) 353, 385
AluBI AGCT 5 cut(s) 212, 320, 364, 643, 782
AluI AGCT 5 cut(s) 212, 320, 364, 643, 782
Alw21I GWGCWC 1 cut(s) 999
Alw26I GTCTC 2 cut(s) 391, 424
AlwI GGATC 2 cut(s) 71, 80
AlwNI CAGNNNCTG 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 1 cut(s) 53
ApeKI GCWGC 5 cut(s) 212, 320, 782, 861, 870
ApoI RAATTY 4 cut(s) 26, 528, 705, 1031
Asp718I GGTACC 1 cut(s) 818
AspA2I CCTAGG 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 689, 724
AsuC2I CCSGG 3 cut(s) 720, 721, 909
AsuHPI GGTGA 1 cut(s) 923
AvaI CYCGRG 1 cut(s) 719
AvaII GGWCC 2 cut(s) 689, 724
AvrII CCTAGG 1 cut(s) 488
AxyI CCTNAGG 1 cut(s) 798
BanI GGYRCC 1 cut(s) 818
BarI GAAGNNNNNNTAC 2 cut(s) 664, 696
BauI CACGAG 1 cut(s) 233
BbsI GAAGAC 2 cut(s) 609, 1032
Bbv12I GWGCWC 1 cut(s) 999
BbvI GCAGC 5 cut(s) 199, 307, 769, 873, 882
BccI CCATC 3 cut(s) 232, 680, 701
BceAI ACGGC 1 cut(s) 377
BciT130I CCWGG 2 cut(s) 57, 816
BcnI CCSGG 3 cut(s) 720, 721, 909
BcoDI GTCTC 2 cut(s) 391, 424
BfaI CTAG 1 cut(s) 489
BfmI CTRYAG 2 cut(s) 204, 315
BfuAI ACCTGC 1 cut(s) 598
BisI GCNGC 5 cut(s) 213, 321, 783, 862, 871
BlnI CCTAGG 1 cut(s) 488
BlsI GCNGC 5 cut(s) 214, 322, 784, 863, 872
Bme1390I CCNGG 5 cut(s) 57, 720, 721, 816, 909
Bme18I GGWCC 2 cut(s) 689, 724
BmeT110I CYCGRG 1 cut(s) 719
BmgT120I GGNCC 2 cut(s) 689, 724
BmiI GGNNCC 4 cut(s) 691, 725, 726, 820
BmrFI CCNGG 5 cut(s) 57, 720, 721, 816, 909
BmsI GCATC 4 cut(s) 459, 512, 838, 913
BpiI GAAGAC 2 cut(s) 609, 1032
BplI GAGNNNNNCTC 2 cut(s) 981, 1013
BpmI CTGGAG 1 cut(s) 430
BpuMI CCSGG 3 cut(s) 720, 721, 909
Bsa29I ATCGAT 1 cut(s) 75
BsaJI CCNNGG 5 cut(s) 109, 393, 488, 719, 720
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 2 cut(s) 42, 447
Bse21I CCTNAGG 1 cut(s) 798
Bse3DI GCAATG 1 cut(s) 22
BseBI CCWGG 2 cut(s) 57, 816
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 5 cut(s) 109, 393, 488, 719, 720
BseGI GGATG 4 cut(s) 481, 552, 853, 904
BseMI GCAATG 1 cut(s) 22
BseMII CTCAG 1 cut(s) 789
BseNI ACTGG 2 cut(s) 42, 447
BseRI GAGGAG 2 cut(s) 535, 792
BseXI GCAGC 5 cut(s) 199, 307, 769, 873, 882
BseYI CCCAGC 1 cut(s) 364
BshFI GGCC 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 818
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 999
BsiHKCI CYCGRG 1 cut(s) 719
BsiSI CCGG 3 cut(s) 720, 858, 908
BslFI GGGAC 2 cut(s) 675, 710
BsmAI GTCTC 2 cut(s) 391, 424
BsmFI GGGAC 2 cut(s) 675, 710
BsnI GGCC 1 cut(s) 55
BsoBI CYCGRG 1 cut(s) 719
Bsp1286I GDGCHC 1 cut(s) 999
Bsp143I GATC 2 cut(s) 72, 76
BspACI CCGC 1 cut(s) 410
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 1 cut(s) 790
BspDI ATCGAT 1 cut(s) 75
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 4 cut(s) 691, 725, 726, 820
BspMAI CTGCAG 1 cut(s) 208
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 2 cut(s) 71, 80
BspT107I GGYRCC 1 cut(s) 818
BsrDI GCAATG 1 cut(s) 22
BsrFI RCCGGY 1 cut(s) 857
BsrI ACTGG 2 cut(s) 42, 447
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 5 cut(s) 109, 393, 488, 719, 720
BssMI GATC 2 cut(s) 72, 76
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 488
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 2 cut(s) 57, 816
Bst4CI ACNGT 3 cut(s) 124, 463, 823
Bst6I CTCTTC 1 cut(s) 893
BstAPI GCANNNNNTGC 1 cut(s) 212
BstC8I GCNNGC 2 cut(s) 217, 1011
BstDEI CTNAG 3 cut(s) 227, 494, 798
BstDSI CCRYGG 2 cut(s) 109, 393
BstF5I GGATG 4 cut(s) 481, 552, 853, 904
BstKTI GATC 2 cut(s) 75, 79
BstMAI GTCTC 2 cut(s) 391, 424
BstMBI GATC 2 cut(s) 72, 76
BstMWI GCNNNNNNNGC 4 cut(s) 212, 329, 867, 870
BstNI CCWGG 2 cut(s) 57, 816
BstSCI CCNGG 5 cut(s) 55, 718, 719, 814, 907
BstSFI CTRYAG 2 cut(s) 204, 315
BstV1I GCAGC 5 cut(s) 199, 307, 769, 873, 882
BstV2I GAAGAC 2 cut(s) 609, 1032
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 1 cut(s) 798
BsuRI GGCC 1 cut(s) 55
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 2 cut(s) 109, 393
BtgZI GCGATG 1 cut(s) 174
BtsCI GGATG 4 cut(s) 481, 552, 853, 904
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 49, 201
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 217, 1011
CaiI CAGNNNCTG 1 cut(s) 212
CciI TCATGA 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 2 cut(s) 689, 724
Cfr9I CCCGGG 1 cut(s) 719
ClaI ATCGAT 1 cut(s) 75
CsiI ACCWGGT 1 cut(s) 814
Csp6I GTAC 4 cut(s) 114, 812, 819, 855
CviAII CATG 1 cut(s) 49
CviJI RGCY 9 cut(s) 55, 212, 289, 320, 364, 493, 643, 782, 972
CviKI_1 RGCY 9 cut(s) 55, 212, 289, 320, 364, 493, 643, 782, 972
CviQI GTAC 4 cut(s) 114, 812, 819, 855
DdeI CTNAG 3 cut(s) 227, 494, 798
DpnI GATC 2 cut(s) 74, 78
DpnII GATC 2 cut(s) 72, 76
DraI TTTAAA 1 cut(s) 732
Eam1104I CTCTTC 1 cut(s) 893
EarI CTCTTC 1 cut(s) 893
Eco130I CCWWGG 1 cut(s) 488
Eco147I AGGCCT 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 689, 724
Eco81I CCTNAGG 1 cut(s) 798
Eco88I CYCGRG 1 cut(s) 719
EcoO109I RGGNCCY 1 cut(s) 724
EcoRII CCWGG 2 cut(s) 55, 814
EcoT14I CCWWGG 1 cut(s) 488
EcoT22I ATGCAT 1 cut(s) 985
ErhI CCWWGG 1 cut(s) 488
FaeI CATG 1 cut(s) 52
FaqI GGGAC 2 cut(s) 675, 710
FatI CATG 1 cut(s) 48
FblI GTMKAC 1 cut(s) 825
Fnu4HI GCNGC 5 cut(s) 213, 321, 783, 862, 871
FokI GGATG 4 cut(s) 488, 559, 860, 891
Fsp4HI GCNGC 5 cut(s) 213, 321, 783, 862, 871
FspBI CTAG 1 cut(s) 489
GluI GCNGC 5 cut(s) 213, 321, 783, 862, 871
GsaI CCCAGC 1 cut(s) 368
GsuI CTGGAG 1 cut(s) 430
HaeIII GGCC 1 cut(s) 55
HapII CCGG 3 cut(s) 720, 858, 908
Hin1II CATG 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 2 cut(s) 38, 105
HpaII CCGG 3 cut(s) 720, 858, 908
HphI GGTGA 1 cut(s) 923
Hpy166II GTNNAC 2 cut(s) 674, 826
Hpy188I TCNGA 6 cut(s) 308, 639, 649, 685, 924, 1018
Hpy188III TCNNGA 4 cut(s) 8, 22, 49, 233
Hpy8I GTNNAC 2 cut(s) 674, 826
HpyAV CCTTC 2 cut(s) 556, 870
HpyCH4III ACNGT 3 cut(s) 124, 463, 823
HpyCH4IV ACGT 2 cut(s) 173, 453
HpyCH4V TGCA 8 cut(s) 206, 332, 404, 472, 602, 625, 656, 983
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 329, 867, 870
HpyF3I CTNAG 3 cut(s) 227, 494, 798
HpySE526I ACGT 2 cut(s) 173, 453
Hsp92II CATG 1 cut(s) 52
KflI GGGWCCC 1 cut(s) 724
KpnI GGTACC 1 cut(s) 822
Kzo9I GATC 2 cut(s) 72, 76
LmnI GCTCC 6 cut(s) 345, 361, 449, 666, 721, 779
Lsp1109I GCAGC 5 cut(s) 199, 307, 769, 873, 882
LweI GCATC 4 cut(s) 459, 512, 838, 913
MabI ACCWGGT 1 cut(s) 814
MaeI CTAG 1 cut(s) 489
MaeII ACGT 2 cut(s) 173, 453
MaeIII GTNAC 6 cut(s) 169, 230, 449, 454, 592, 745
MalI GATC 2 cut(s) 74, 78
MboI GATC 2 cut(s) 72, 76
MboII GAAGA 6 cut(s) 144, 578, 609, 672, 910, 1037
MhlI GDGCHC 1 cut(s) 999
MluCI AATT 7 cut(s) 26, 381, 528, 705, 803, 948, 1031
Mph1103I ATGCAT 1 cut(s) 985
MseI TTAA 8 cut(s) 279, 284, 357, 534, 731, 768, 986, 1039
MslI CAYNNNNRTG 3 cut(s) 909, 963, 978
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 3 cut(s) 720, 858, 908
MspR9I CCNGG 5 cut(s) 57, 720, 721, 816, 909
MvaI CCWGG 2 cut(s) 57, 816
MwoI GCNNNNNNNGC 4 cut(s) 212, 329, 867, 870
NciI CCSGG 3 cut(s) 720, 721, 909
NdeII GATC 2 cut(s) 72, 76
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 4 cut(s) 691, 725, 726, 820
NmuCI GTSAC 3 cut(s) 169, 230, 454
NsiI ATGCAT 1 cut(s) 985
OliI CACNNNNGTG 1 cut(s) 963
PagI TCATGA 1 cut(s) 48
PceI AGGCCT 1 cut(s) 55
PfeI GAWTC 2 cut(s) 38, 105
PkrI GCNGC 5 cut(s) 214, 322, 784, 863, 872
PpuMI RGGWCCY 1 cut(s) 724
PsiI TTATAA 1 cut(s) 137
Psp5II RGGWCCY 1 cut(s) 724
Psp6I CCWGG 2 cut(s) 55, 814
PspFI CCCAGC 1 cut(s) 364
PspGI CCWGG 2 cut(s) 55, 814
PspN4I GGNNCC 4 cut(s) 691, 725, 726, 820
PspPI GGNCC 2 cut(s) 689, 724
PspPPI RGGWCCY 1 cut(s) 724
PstI CTGCAG 1 cut(s) 208
PstNI CAGNNNCTG 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 4 cut(s) 115, 813, 820, 856
RsaNI GTAC 4 cut(s) 114, 812, 819, 855
RseI CAYNNNNRTG 3 cut(s) 909, 963, 978
SaqAI TTAA 8 cut(s) 279, 284, 357, 534, 731, 768, 986, 1039
SatI GCNGC 5 cut(s) 213, 321, 783, 862, 871
Sau3AI GATC 2 cut(s) 72, 76
Sau96I GGNCC 2 cut(s) 689, 724
ScrFI CCNGG 5 cut(s) 57, 720, 721, 816, 909
SduI GDGCHC 1 cut(s) 999
SexAI ACCWGGT 1 cut(s) 814
SfaNI GCATC 4 cut(s) 459, 512, 838, 913
SfcI CTRYAG 2 cut(s) 204, 315
SinI GGWCC 2 cut(s) 689, 724
SmaI CCCGGG 1 cut(s) 721
SmiMI CAYNNNNRTG 3 cut(s) 909, 963, 978
Sse9I AATT 7 cut(s) 26, 381, 528, 705, 803, 948, 1031
SseBI AGGCCT 1 cut(s) 55
SsiI CCGC 1 cut(s) 410
SspMI CTAG 1 cut(s) 489
StuI AGGCCT 1 cut(s) 55
StyD4I CCNGG 5 cut(s) 55, 718, 719, 814, 907
StyI CCWWGG 1 cut(s) 488
TaaI ACNGT 3 cut(s) 124, 463, 823
TaiI ACGT 2 cut(s) 176, 456
TaqI TCGA 1 cut(s) 75
TasI AATT 7 cut(s) 26, 381, 528, 705, 803, 948, 1031
TfiI GAWTC 2 cut(s) 38, 105
Tru1I TTAA 8 cut(s) 279, 284, 357, 534, 731, 768, 986, 1039
Tru9I TTAA 8 cut(s) 279, 284, 357, 534, 731, 768, 986, 1039
TscAI CASTG 2 cut(s) 49, 208
TseFI GTSAC 3 cut(s) 169, 230, 454
TseI GCWGC 5 cut(s) 212, 320, 782, 861, 870
Tsp45I GTSAC 3 cut(s) 169, 230, 454
TspDTI ATGAA 2 cut(s) 180, 1038
TspGWI ACGGA 2 cut(s) 98, 332
TspMI CCCGGG 1 cut(s) 719
TspRI CASTG 2 cut(s) 49, 208
VpaK11BI GGWCC 2 cut(s) 689, 724
XapI RAATTY 4 cut(s) 26, 528, 705, 1031
XmaI CCCGGG 1 cut(s) 719
XmaJI CCTAGG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 825
XspI CTAG 1 cut(s) 489
Zsp2I ATGCAT 1 cut(s) 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.