pycom05g19220

Protein OSB2, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012578
Physical Location & Seq
Reverse (-)
22095997 .. 22098328
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 987 bp
ATGTGGTCCAAACCTTCCAATAACCCTTGGGGAGTTCAGATGAAACGCAGTCTAAGTAGCATTATCCGACAACGGCGGAGTTTTGGCGCCATCCCCCAAAAAACGCCGAGAGCAATGGTGTTAGAGCGAGCAATTCCGGCAGCAACAAGCACAACCTTCCTCGCCAACCCCAAAGTTCCCGCCTTTTTCCCAACTACCAGCCACTTGGCCTTCGCACGGCGCCGTTTCAATCTCAAATGCTCGATGGAGTACAGTAGAGACCAGTACAACGGCAGCGGGAGCAGCATTCAGGTGCCGATAGCTTTCCCTAGACCGGCGGAGGTCCAGTGGAAAAAGGAGCTGTGCAATATGGTGCACCTCATCGGCGTCGTCGGTCTTCCCGTCGAAATAAAGCACCTCCCCTCCGGAAAGGTCCTCGCTTGGACCCGCCTCGCCGTCAAGAAGTCCGCCACCGATACCTCTTGGATCAATCTGACATTTTGGGATGAGCTTGCACATGTTGCTTTTCAGCATGTAGAGAAAGGCCAACAAATATATGTATCGGGCCGGCTGATATCAGATTCTGTTGAAACCGATGAAGGGAAGCAGCAGACCTATTATAAGGTAGTTGTTCAGCAACTGAATTTTGTTGAAAAGGGCTTTTCATCGCCGGCCATGAAAGATCACGACTCTGATTCCTTTACAGCGGGCAGAAATCTTGGCAGCAATGCCACAAAGAACATAACGGAAACCACTCAAGAATTATGGCAAGCCTTCTTTGCTAATCCAGTTGACTGGTGGGATAATCGGAAGACCAAGAGGAACCCAAAATATCCGGATTTTAAGCATAAAGATACTGGAGAAGCCTTGTGGATTGAAGGCCGGAACAATCCGCCATGGGTGAAGTCCCAACTTGCTATACTGGATGCAAGAATGGGGACCCTTAATGACCAAGAACATAACCTGTATACAGATATATTTGCCGGTGATAGTTTTTCGCCTTTCTAA

Protein Analysis

329

Amino Acids

37.28

Weight (kDa)

9.52

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 116 - 209 8.3e-17 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
AccB1I GGYRCC 3 cut(s) 86, 219, 292
AccI GTMKAC 1 cut(s) 947
AccIII TCCGGA 2 cut(s) 404, 814
AciI CCGC 8 cut(s) 76, 180, 276, 317, 427, 447, 686, 872
AclWI GGATC 1 cut(s) 473
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 1 cut(s) 622
AcyI GRCGYC 3 cut(s) 87, 220, 366
AfaI GTAC 2 cut(s) 251, 266
AfiI CCNNNNNNNGG 3 cut(s) 216, 313, 579
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 4 cut(s) 229, 569, 632, 857
AluBI AGCT 3 cut(s) 302, 340, 490
AluI AGCT 3 cut(s) 302, 340, 490
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 1 cut(s) 252
Alw44I GTGCAC 1 cut(s) 353
AlwI GGATC 1 cut(s) 473
AlwNI CAGNNNCTG 2 cut(s) 563, 619
Aor13HI TCCGGA 2 cut(s) 404, 814
AoxI GGCC 5 cut(s) 207, 523, 544, 651, 859
ApaLI GTGCAC 1 cut(s) 353
ApeKI GCWGC 5 cut(s) 140, 273, 282, 586, 702
ApoI RAATTY 1 cut(s) 622
AspLEI GCGC 2 cut(s) 89, 222
AspS9I GGNCC 6 cut(s) 6, 322, 412, 423, 544, 918
AsuHPI GGTGA 2 cut(s) 892, 977
AvaII GGWCC 5 cut(s) 6, 322, 412, 423, 918
BaeGI GKGCMC 1 cut(s) 357
BanI GGYRCC 3 cut(s) 86, 219, 292
BbsI GAAGAC 2 cut(s) 368, 797
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 5 cut(s) 152, 285, 294, 598, 714
BccI CCATC 2 cut(s) 98, 238
BceAI ACGGC 5 cut(s) 89, 207, 233, 286, 419
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 1 cut(s) 309
BfoI RGCGCY 2 cut(s) 90, 223
BisI GCNGC 5 cut(s) 141, 274, 283, 587, 703
BlsI GCNGC 5 cut(s) 142, 275, 284, 588, 704
Bme18I GGWCC 5 cut(s) 6, 322, 412, 423, 918
BmgT120I GGNCC 6 cut(s) 6, 322, 412, 423, 544, 918
BmiI GGNNCC 7 cut(s) 88, 221, 294, 425, 803, 919, 920
BmsI GCATC 1 cut(s) 895
BpiI GAAGAC 2 cut(s) 368, 797
BpmI CTGGAG 1 cut(s) 858
BpuEI CTTGAG 1 cut(s) 720
BsaHI GRCGYC 3 cut(s) 87, 220, 366
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 2 cut(s) 26, 875
BsaWI WCCGGW 2 cut(s) 404, 814
BsaXI ACNNNNNCTCC 4 cut(s) 311, 341, 386, 416
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 313, 579
Bse118I RCCGGY 4 cut(s) 313, 546, 649, 962
Bse1I ACTGG 6 cut(s) 262, 325, 767, 779, 841, 906
Bse3DI GCAATG 2 cut(s) 120, 712
BseAI TCCGGA 2 cut(s) 404, 814
BseDI CCNNGG 2 cut(s) 26, 875
BseGI GGATG 3 cut(s) 90, 490, 910
BseLI CCNNNNNNNGG 3 cut(s) 216, 313, 579
BseMI GCAATG 2 cut(s) 120, 712
BseNI ACTGG 6 cut(s) 262, 325, 767, 779, 841, 906
BseSI GKGCMC 1 cut(s) 357
BseXI GCAGC 5 cut(s) 152, 285, 294, 598, 714
BshFI GGCC 5 cut(s) 209, 525, 546, 653, 861
BshNI GGYRCC 3 cut(s) 86, 219, 292
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 8 cut(s) 137, 314, 405, 547, 650, 815, 862, 963
BslFI GGGAC 2 cut(s) 871, 931
BslI CCNNNNNNNGG 3 cut(s) 216, 313, 579
BsmAI GTCTC 1 cut(s) 252
BsmFI GGGAC 2 cut(s) 871, 931
BsmI GAATGC 1 cut(s) 285
BsnI GGCC 5 cut(s) 209, 525, 546, 653, 861
Bso31I GGTCTC 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 357
Bsp13I TCCGGA 2 cut(s) 404, 814
Bsp143I GATC 2 cut(s) 465, 661
Bsp19I CCATGG 1 cut(s) 875
BspACI CCGC 8 cut(s) 76, 180, 276, 317, 427, 447, 686, 872
BspANI GGCC 5 cut(s) 209, 525, 546, 653, 861
BspEI TCCGGA 2 cut(s) 404, 814
BspLI GGNNCC 7 cut(s) 88, 221, 294, 425, 803, 919, 920
BspPI GGATC 1 cut(s) 473
BspT107I GGYRCC 3 cut(s) 86, 219, 292
BspTNI GGTCTC 1 cut(s) 252
BsrDI GCAATG 2 cut(s) 120, 712
BsrFI RCCGGY 4 cut(s) 313, 546, 649, 962
BsrI ACTGG 6 cut(s) 262, 325, 767, 779, 841, 906
BssAI RCCGGY 4 cut(s) 313, 546, 649, 962
BssECI CCNNGG 2 cut(s) 26, 875
BssMI GATC 2 cut(s) 465, 661
BssNAI GTATAC 1 cut(s) 948
BssNI GRCGYC 3 cut(s) 87, 220, 366
BssT1I CCWWGG 2 cut(s) 26, 875
Bst1107I GTATAC 1 cut(s) 948
Bst4CI ACNGT 1 cut(s) 254
BstACI GRCGYC 3 cut(s) 87, 220, 366
BstAPI GCANNNNNTGC 1 cut(s) 500
BstC8I GCNNGC 6 cut(s) 129, 492, 548, 651, 688, 750
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 1 cut(s) 875
BstF5I GGATG 3 cut(s) 90, 490, 910
BstH2I RGCGCY 2 cut(s) 90, 223
BstHHI GCGC 2 cut(s) 89, 222
BstKTI GATC 2 cut(s) 468, 664
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 2 cut(s) 465, 661
BstMWI GCNNNNNNNGC 5 cut(s) 137, 279, 282, 500, 758
BstNSI RCATGY 2 cut(s) 500, 515
BstSLI GKGCMC 1 cut(s) 357
BstV1I GCAGC 5 cut(s) 152, 285, 294, 598, 714
BstV2I GAAGAC 2 cut(s) 368, 797
BstXI CCANNNNNNTGG 2 cut(s) 205, 774
BstZ17I GTATAC 1 cut(s) 948
BsuRI GGCC 5 cut(s) 209, 525, 546, 653, 861
BtgI CCRYGG 1 cut(s) 875
BtgZI GCGATG 1 cut(s) 630
BtsCI GGATG 3 cut(s) 90, 490, 910
BtsIMutI CAGTG 1 cut(s) 332
Cac8I GCNNGC 6 cut(s) 129, 492, 548, 651, 688, 750
CaiI CAGNNNCTG 2 cut(s) 563, 619
CfoI GCGC 2 cut(s) 89, 222
Cfr10I RCCGGY 4 cut(s) 313, 546, 649, 962
Cfr13I GGNCC 6 cut(s) 6, 322, 412, 423, 544, 918
CseI GACGC 1 cut(s) 355
Csp6I GTAC 2 cut(s) 250, 265
CviAII CATG 4 cut(s) 497, 512, 655, 876
CviQI GTAC 2 cut(s) 250, 265
DdeI CTNAG 1 cut(s) 53
DinI GGCGCC 2 cut(s) 88, 221
DpnI GATC 2 cut(s) 467, 663
DpnII GATC 2 cut(s) 465, 661
EaeI YGGCCR 1 cut(s) 651
EciI GGCGGA 4 cut(s) 91, 332, 436, 861
Eco130I CCWWGG 2 cut(s) 26, 875
Eco31I GGTCTC 1 cut(s) 252
Eco32I GATATC 1 cut(s) 555
Eco47I GGWCC 5 cut(s) 6, 322, 412, 423, 918
EcoO109I RGGNCCY 2 cut(s) 412, 918
EcoRV GATATC 1 cut(s) 555
EcoT14I CCWWGG 2 cut(s) 26, 875
EgeI GGCGCC 2 cut(s) 88, 221
EheI GGCGCC 2 cut(s) 88, 221
ErhI CCWWGG 2 cut(s) 26, 875
FaeI CATG 4 cut(s) 500, 515, 658, 879
FaqI GGGAC 2 cut(s) 871, 931
FatI CATG 4 cut(s) 496, 511, 654, 875
FauI CCCGC 4 cut(s) 187, 269, 434, 679
FblI GTMKAC 1 cut(s) 947
Fnu4HI GCNGC 5 cut(s) 141, 274, 283, 587, 703
FokI GGATG 3 cut(s) 77, 497, 917
Fsp4HI GCNGC 5 cut(s) 141, 274, 283, 587, 703
FspBI CTAG 1 cut(s) 309
GlaI GCGC 2 cut(s) 88, 221
GluI GCNGC 5 cut(s) 141, 274, 283, 587, 703
GsuI CTGGAG 1 cut(s) 858
HaeII RGCGCY 2 cut(s) 90, 223
HaeIII GGCC 5 cut(s) 209, 525, 546, 653, 861
HapII CCGG 8 cut(s) 137, 314, 405, 547, 650, 815, 862, 963
HgaI GACGC 1 cut(s) 355
HhaI GCGC 2 cut(s) 89, 222
Hin1I GRCGYC 3 cut(s) 87, 220, 366
Hin1II CATG 4 cut(s) 500, 515, 658, 879
Hin6I GCGC 2 cut(s) 87, 220
HinP1I GCGC 2 cut(s) 87, 220
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 3 cut(s) 560, 668, 674
HpaII CCGG 8 cut(s) 137, 314, 405, 547, 650, 815, 862, 963
HphI GGTGA 2 cut(s) 892, 977
Hpy166II GTNNAC 3 cut(s) 355, 772, 948
Hpy188I TCNGA 6 cut(s) 39, 68, 474, 559, 673, 789
Hpy188III TCNNGA 5 cut(s) 405, 439, 665, 737, 815
Hpy8I GTNNAC 3 cut(s) 355, 772, 948
Hpy99I CGWCG 3 cut(s) 371, 374, 386
HpyAV CCTTC 6 cut(s) 24, 166, 220, 572, 763, 851
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4V TGCA 4 cut(s) 345, 355, 494, 908
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 279, 282, 500, 758
HpyF3I CTNAG 1 cut(s) 53
Hsp92I GRCGYC 3 cut(s) 87, 220, 366
Hsp92II CATG 4 cut(s) 500, 515, 658, 879
HspAI GCGC 2 cut(s) 87, 220
KasI GGCGCC 2 cut(s) 86, 219
KflI GGGWCCC 1 cut(s) 918
Kpn2I TCCGGA 2 cut(s) 404, 814
KroI GCCGGC 2 cut(s) 546, 649
KroNI GCCGGC 2 cut(s) 548, 651
Kzo9I GATC 2 cut(s) 465, 661
LmnI GCTCC 2 cut(s) 279, 337
Lsp1109I GCAGC 5 cut(s) 152, 285, 294, 598, 714
LweI GCATC 1 cut(s) 895
MaeI CTAG 1 cut(s) 309
MalI GATC 2 cut(s) 467, 663
MboI GATC 2 cut(s) 465, 661
MboII GAAGA 2 cut(s) 368, 802
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 3 cut(s) 132, 622, 740
Mly113I GGCGCC 2 cut(s) 87, 220
MlyI GAGTC 1 cut(s) 662
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 9 cut(s) 170, 313, 368, 407, 412, 425, 440, 469, 792
MroI TCCGGA 2 cut(s) 404, 814
MroNI GCCGGC 2 cut(s) 546, 649
MseI TTAA 2 cut(s) 822, 924
MslI CAYNNNNRTG 1 cut(s) 290
MspA1I CMGCKG 2 cut(s) 276, 686
MspI CCGG 8 cut(s) 137, 314, 405, 547, 650, 815, 862, 963
Mva1269I GAATGC 1 cut(s) 285
MwoI GCNNNNNNNGC 5 cut(s) 137, 279, 282, 500, 758
NaeI GCCGGC 2 cut(s) 548, 651
NarI GGCGCC 2 cut(s) 87, 220
NcoI CCATGG 1 cut(s) 875
NdeII GATC 2 cut(s) 465, 661
NgoMIV GCCGGC 2 cut(s) 546, 649
NlaIII CATG 4 cut(s) 500, 515, 658, 879
NlaIV GGNNCC 7 cut(s) 88, 221, 294, 425, 803, 919, 920
NmeAIII GCCGAG 1 cut(s) 132
NspI RCATGY 2 cut(s) 500, 515
PciI ACATGT 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PctI GAATGC 1 cut(s) 285
PdiI GCCGGC 2 cut(s) 548, 651
PfeI GAWTC 2 cut(s) 560, 674
PkrI GCNGC 5 cut(s) 142, 275, 284, 588, 704
PleI GAGTC 1 cut(s) 662
PluTI GGCGCC 2 cut(s) 90, 223
PpsI GAGTC 1 cut(s) 662
PpuMI RGGWCCY 2 cut(s) 412, 918
PscI ACATGT 1 cut(s) 496
PsiI TTATAA 1 cut(s) 600
Psp5II RGGWCCY 2 cut(s) 412, 918
PspN4I GGNNCC 7 cut(s) 88, 221, 294, 425, 803, 919, 920
PspPI GGNCC 6 cut(s) 6, 322, 412, 423, 544, 918
PspPPI RGGWCCY 2 cut(s) 412, 918
PstNI CAGNNNCTG 2 cut(s) 563, 619
RsaI GTAC 2 cut(s) 251, 266
RsaNI GTAC 2 cut(s) 250, 265
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 2 cut(s) 822, 924
SatI GCNGC 5 cut(s) 141, 274, 283, 587, 703
Sau3AI GATC 2 cut(s) 465, 661
Sau96I GGNCC 6 cut(s) 6, 322, 412, 423, 544, 918
SchI GAGTC 1 cut(s) 662
SduI GDGCHC 1 cut(s) 357
SfaNI GCATC 1 cut(s) 895
SfoI GGCGCC 2 cut(s) 88, 221
SinI GGWCC 5 cut(s) 6, 322, 412, 423, 918
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 735
SmoI CTYRAG 1 cut(s) 735
Sse9I AATT 3 cut(s) 132, 622, 740
SsiI CCGC 8 cut(s) 76, 180, 276, 317, 427, 447, 686, 872
SspDI GGCGCC 2 cut(s) 86, 219
SspMI CTAG 1 cut(s) 309
StyI CCWWGG 2 cut(s) 26, 875
TaaI ACNGT 1 cut(s) 254
TaqI TCGA 2 cut(s) 242, 384
TaqII GACCGA 1 cut(s) 362
TasI AATT 3 cut(s) 132, 622, 740
TatI WGTACW 2 cut(s) 249, 264
TfiI GAWTC 2 cut(s) 560, 674
Tru1I TTAA 2 cut(s) 822, 924
Tru9I TTAA 2 cut(s) 822, 924
TscAI CASTG 1 cut(s) 332
TseI GCWGC 5 cut(s) 140, 273, 282, 586, 702
TspDTI ATGAA 4 cut(s) 56, 591, 633, 671
TspGWI ACGGA 1 cut(s) 740
TspRI CASTG 1 cut(s) 332
VneI GTGCAC 1 cut(s) 353
VpaK11BI GGWCC 5 cut(s) 6, 322, 412, 423, 918
XapI RAATTY 1 cut(s) 622
XceI RCATGY 2 cut(s) 500, 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 774
XmiI GTMKAC 1 cut(s) 947
XspI CTAG 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.