pycom05g21580

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014691
Physical Location & Seq
Forward (+)
24163116 .. 24164711
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21580.1

Sequence Viewer

Length: 1596 bp
ATGGTGCTGCTACTCAAGACCCAGATAAATGGTTTAGCCAGACAAGCAGAGGTTTTAATTTCATGGAAGGAACCCTTCAGATTATTAGGGTTTTTCATTGGTGGCAGAACATACACGACACTTCATTGCAGAGACTCTTATCACTATACCTACTTACTGCAACACTGTAAAAGCACCAATTCGATCAAAAAACTCCATGCCCAGATAACCACTGGAGGTTTTGAGCAAAACCCATTTGTCGTTGCAAAGCTAGTTGGAAAGTATGTGGAGTGCAGTGAATCAAGCATGGAAGCTGCACGGAAGGTGTTCGATAGATTGCTTGAGAGAGATGCTTTTGTGTGGAACATGGTTATTCAGGGCTATGCGAATGTGGGTCCTTTTGTTGAAGCATTCAATGTGTATAATAGAATGCGGTTGAGTGGTGTGCCTGCAAATAGGTACACCTACCCTTTTGTGCTCAAGGCTTGTGGTGCAATGAGAGATGGAAAACGAGGTCGGATTGTTCATGGACATGTTGTGAAATGTGGACTCGACTCGGATTTGTTCGTTGGGAATGCTCTTATTGCCTTGTATTCCAAGTGTGAGGAGATTGAGATTTCGAGAAGAGTGTTTGATGAAATACCTGGGAAAGATTCTGTAAGTTGGAATTCCATTATTTCGGGGTATACGGCAAATGGAAATCCTCACGAGGCTCTCATGCTTTTCCTCACCATGCTGCAAGATCGGGCTGCATGCTTGCCTGACCATGCCACTCTTGTGTGCATTCTTCCGGCTTGTGTTGAAGCATCAGCTATTGAAGTTGGATTTTGGATTCATTCTTACATTATAAAGTCAAGTATGAAAGTCGACGCTGTTTTAGGCAGCGCTCTCATTACAATGTACGCAAATTGTGGCCGCGTAACCACTGCCAGAGTTATTTTTTATCAAATCAGCGAGAAAAATGTGGGGGCGTGGAGTGCGATGATGCGGTGTTATGGAATGCATGGGCATGCAGATGAGGCACTCCAAATGTTTTCGCAGTTTGTGGAGTCTGGCTTACGCCCGGATGGTATTGTATTTTTGTGTTTGCTGTCCACTTGTAGTCACTCGGGGTTCATCACGAAAGGCTTGGAACTTTTTGAGAAAATGGGAGAATATGGAGTAGCGAAAAGCGAGAAACATTATGCTTGTGTGGTCGACCTTCTGGGAAGAGCTGGTTTACTTGACCAGGCGGTCGAACTTATCAGAACCATGCCCATGGAAGCAGGTAAAGATGTATATGGGGCCTTACTGGGAGCTTGTAGAATACATAATAACATGGAACTCGCCGAAAAAGCTGCAGAAAAGTTGTTTGTTTTGGACCCTGAAAATTCTGGGAGGTATATTCTTCTGGCCAGCATGTATGAAGATGCAGGTAGATGGGAGGATGCTGGTAGAGTGAGGAAGCTACTGAGAGACAACAACGTCAAGAAACCAACCGGAAGTAGTTCAATCGAGGTGGAATGTATGTATCACACATTTGGAGCAGATGACGAATCTCACCCATATACAGACCAGATTTTCAACACATTGCAGAGACTGGATAGGATAATGGAAGAAGAAACACTGAAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

532

Amino Acids

59.47

Weight (kDa)

6.61

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 110 - 156 7.6e-10 PPR repeat family
PPR_2 PF13041 210 - 259 2.7e-07 PPR repeat family
PPR PF01535 213 - 240 1.8e-06 PPR repeat
E_motif PF20431 430 - 492 8.5e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014691)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g13960
malus_domestica MD05G1239500.v1.1 MD10G1217700.v1.1
prunus_persica Prupe.4G126300_v2.0.a1
pyrus_communis pycom05g21580 pycom10g18360
rosa_chinensis RchiOBHm_Chr5g0023171
rosa_laevigata RLG00000032705
rosa_multiflora Rmu_sc0007300.1_g000014
rosa_roxburghii Rroxscaffold_1G00056170
rosa_rugosa Rorug05G0077700
rosa_samantha Rh5AG163900 Rh5BG163200 Rh5CG178200 Rh5DG164600
rosa_wichuraiana Rw0G018300 Rw5G014790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 827
AasI GACNNNNNNGTC 2 cut(s) 1211, 1442
Acc36I ACCTGC 2 cut(s) 1235, 1382
AccI GTMKAC 3 cut(s) 665, 846, 1176
AccII CGCG 1 cut(s) 897
AciI CCGC 4 cut(s) 412, 895, 967, 1211
AcoI YGGCCR 2 cut(s) 892, 1371
AcsI RAATTY 2 cut(s) 646, 1348
AcuI CTGAAG 1 cut(s) 61
AfaI GTAC 2 cut(s) 440, 881
AfeI AGCGCT 1 cut(s) 865
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 6 cut(s) 386, 394, 782, 797, 1470, 1543
AjnI CCWGG 2 cut(s) 622, 1206
AleI CACNNNNGTG 1 cut(s) 755
AluBI AGCT 7 cut(s) 250, 293, 791, 1193, 1277, 1316, 1426
AluI AGCT 7 cut(s) 250, 293, 791, 1193, 1277, 1316, 1426
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 3 cut(s) 126, 1428, 1549
AlwNI CAGNNNCTG 1 cut(s) 1558
Ama87I CYCGRG 1 cut(s) 1087
Aor51HI AGCGCT 1 cut(s) 865
AoxI GGCC 3 cut(s) 892, 1263, 1371
ApeKI GCWGC 6 cut(s) 7, 293, 715, 728, 861, 1316
ApoI RAATTY 2 cut(s) 646, 1348
Asp700I GAANNNNTTC 2 cut(s) 305, 1465
AspLEI GCGC 1 cut(s) 866
AspS9I GGNCC 3 cut(s) 374, 1263, 1339
AsuC2I CCSGG 1 cut(s) 1043
AsuHPI GGTGA 2 cut(s) 700, 1511
AvaI CYCGRG 1 cut(s) 1087
AvaII GGWCC 2 cut(s) 374, 1339
BalI TGGCCA 1 cut(s) 1373
BauI CACGAG 1 cut(s) 686
Bbv12I GWGCWC 1 cut(s) 459
BbvI GCAGC 5 cut(s) 280, 702, 715, 873, 1303
BccI CCATC 3 cut(s) 476, 1040, 1392
BceAI ACGGC 1 cut(s) 684
BcgI CGANNNNNNTGC 2 cut(s) 1298, 1332
BciT130I CCWGG 2 cut(s) 624, 1208
BcnI CCSGG 1 cut(s) 1043
BcoDI GTCTC 3 cut(s) 126, 1428, 1549
BfaI CTAG 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 1317
BfoI RGCGCY 1 cut(s) 867
BfuAI ACCTGC 2 cut(s) 1235, 1382
BisI GCNGC 7 cut(s) 8, 294, 716, 729, 862, 895, 1317
BlsI GCNGC 7 cut(s) 9, 295, 717, 730, 863, 896, 1318
Bme1390I CCNGG 3 cut(s) 624, 1043, 1208
Bme18I GGWCC 2 cut(s) 374, 1339
BmeT110I CYCGRG 1 cut(s) 1087
BmgT120I GGNCC 3 cut(s) 374, 1263, 1339
BmiI GGNNCC 4 cut(s) 72, 375, 1264, 1341
BmrFI CCNGG 3 cut(s) 624, 1043, 1208
BmrI ACTGGG 1 cut(s) 1280
BmsI GCATC 5 cut(s) 319, 794, 954, 1378, 1396
BmuI ACTGGG 1 cut(s) 1280
BpmI CTGGAG 1 cut(s) 234
BpuEI CTTGAG 2 cut(s) 341, 443
BpuMI CCSGG 1 cut(s) 1043
BsaJI CCNNGG 2 cut(s) 623, 1236
BsaWI WCCGGW 1 cut(s) 1457
Bse1I ACTGG 3 cut(s) 217, 1275, 1563
Bse3DI GCAATG 3 cut(s) 124, 480, 1547
BseBI CCWGG 2 cut(s) 624, 1208
BseDI CCNNGG 2 cut(s) 623, 1236
BseGI GGATG 2 cut(s) 1051, 1411
BseMI GCAATG 3 cut(s) 124, 480, 1547
BseMII CTCAG 1 cut(s) 1421
BseNI ACTGG 3 cut(s) 217, 1275, 1563
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 5 cut(s) 280, 702, 715, 873, 1303
BsgI GTGCAG 2 cut(s) 279, 292
Bsh1236I CGCG 1 cut(s) 897
Bsh1285I CGRYCG 1 cut(s) 1215
BshFI GGCC 3 cut(s) 894, 1265, 1373
BsiEI CGRYCG 1 cut(s) 1215
BsiHKAI GWGCWC 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 1087
BsiSI CCGG 3 cut(s) 770, 1043, 1458
BsmAI GTCTC 3 cut(s) 126, 1428, 1549
BsmI GAATGC 5 cut(s) 389, 414, 559, 762, 984
BsnI GGCC 3 cut(s) 894, 1265, 1373
BsoBI CYCGRG 1 cut(s) 1087
Bsp1286I GDGCHC 1 cut(s) 459
Bsp143I GATC 2 cut(s) 183, 721
Bsp19I CCATGG 1 cut(s) 1236
BspACI CCGC 4 cut(s) 412, 895, 967, 1211
BspANI GGCC 3 cut(s) 894, 1265, 1373
BspCNI CTCAG 1 cut(s) 1422
BspFNI CGCG 1 cut(s) 897
BspLI GGNNCC 4 cut(s) 72, 375, 1264, 1341
BspMAI CTGCAG 1 cut(s) 1321
BspMI ACCTGC 2 cut(s) 1235, 1382
BspQI GCTCTTC 1 cut(s) 1183
BsrDI GCAATG 3 cut(s) 124, 480, 1547
BsrI ACTGG 3 cut(s) 217, 1275, 1563
BssECI CCNNGG 2 cut(s) 623, 1236
BssMI GATC 2 cut(s) 183, 721
BssNAI GTATAC 1 cut(s) 666
BssSI CACGAG 1 cut(s) 686
BssT1I CCWWGG 1 cut(s) 1236
Bst1107I GTATAC 1 cut(s) 666
Bst2BI CACGAG 1 cut(s) 686
Bst2UI CCWGG 2 cut(s) 624, 1208
Bst4CI ACNGT 2 cut(s) 167, 1593
Bst6I CTCTTC 2 cut(s) 598, 1183
BstC8I GCNNGC 5 cut(s) 429, 733, 737, 990, 1375
BstDEI CTNAG 1 cut(s) 1430
BstDSI CCRYGG 1 cut(s) 1236
BstF5I GGATG 2 cut(s) 1051, 1411
BstFNI CGCG 1 cut(s) 897
BstH2I RGCGCY 1 cut(s) 867
BstHHI GCGC 1 cut(s) 866
BstKTI GATC 2 cut(s) 186, 724
BstMAI GTCTC 3 cut(s) 126, 1428, 1549
BstMBI GATC 2 cut(s) 183, 721
BstMCI CGRYCG 1 cut(s) 1215
BstMWI GCNNNNNNNGC 6 cut(s) 44, 470, 563, 956, 998, 1313
BstNI CCWGG 2 cut(s) 624, 1208
BstNSI RCATGY 4 cut(s) 515, 735, 992, 1381
BstSCI CCNGG 3 cut(s) 622, 1041, 1206
BstSFI CTRYAG 1 cut(s) 1317
BstUI CGCG 1 cut(s) 897
BstV1I GCAGC 5 cut(s) 280, 702, 715, 873, 1303
BstXI CCANNNNNNTGG 2 cut(s) 29, 1237
BstZ17I GTATAC 1 cut(s) 666
BsuRI GGCC 3 cut(s) 894, 1265, 1373
BtgI CCRYGG 1 cut(s) 1236
BtgZI GCGATG 1 cut(s) 974
BtsCI GGATG 2 cut(s) 1051, 1411
BtsI GCAGTG 2 cut(s) 280, 903
BtsIMutI CAGTG 5 cut(s) 163, 210, 280, 903, 1583
BveI ACCTGC 2 cut(s) 1235, 1382
Cac8I GCNNGC 5 cut(s) 429, 733, 737, 990, 1375
CaiI CAGNNNCTG 1 cut(s) 1558
CfoI GCGC 1 cut(s) 866
Cfr13I GGNCC 3 cut(s) 374, 1263, 1339
CseI GACGC 1 cut(s) 857
Csp6I GTAC 2 cut(s) 439, 880
CspCI CAANNNNNGTGG 4 cut(s) 448, 483, 1458, 1493
CviQI GTAC 2 cut(s) 439, 880
DdeI CTNAG 1 cut(s) 1430
DpnI GATC 2 cut(s) 185, 723
DpnII GATC 2 cut(s) 183, 721
DrdI GACNNNNNNGTC 2 cut(s) 1211, 1442
DseDI GACNNNNNNGTC 2 cut(s) 1211, 1442
EaeI YGGCCR 2 cut(s) 892, 1371
Eam1104I CTCTTC 2 cut(s) 598, 1183
EarI CTCTTC 2 cut(s) 598, 1183
Eco130I CCWWGG 1 cut(s) 1236
Eco47I GGWCC 2 cut(s) 374, 1339
Eco47III AGCGCT 1 cut(s) 865
Eco57I CTGAAG 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 1087
EcoO109I RGGNCCY 2 cut(s) 374, 1263
EcoRI GAATTC 1 cut(s) 646
EcoRII CCWGG 2 cut(s) 622, 1206
EcoT14I CCWWGG 1 cut(s) 1236
EcoT22I ATGCAT 1 cut(s) 984
ErhI CCWWGG 1 cut(s) 1236
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FblI GTMKAC 3 cut(s) 665, 846, 1176
Fnu4HI GCNGC 7 cut(s) 8, 294, 716, 729, 862, 895, 1317
FokI GGATG 2 cut(s) 1058, 1418
Fsp4HI GCNGC 7 cut(s) 8, 294, 716, 729, 862, 895, 1317
FspBI CTAG 1 cut(s) 251
GlaI GCGC 1 cut(s) 865
GluI GCNGC 7 cut(s) 8, 294, 716, 729, 862, 895, 1317
GsuI CTGGAG 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 867
HaeIII GGCC 3 cut(s) 894, 1265, 1373
HapII CCGG 3 cut(s) 770, 1043, 1458
HgaI GACGC 1 cut(s) 857
HhaI GCGC 1 cut(s) 866
Hin6I GCGC 1 cut(s) 864
HinP1I GCGC 1 cut(s) 864
HincII GTYRAC 2 cut(s) 847, 1177
HindII GTYRAC 2 cut(s) 847, 1177
HinfI GANTC 8 cut(s) 134, 278, 528, 533, 632, 811, 1028, 1514
HpaII CCGG 3 cut(s) 770, 1043, 1458
HphI GGTGA 2 cut(s) 700, 1511
Hpy166II GTNNAC 7 cut(s) 441, 527, 666, 847, 1074, 1177, 1199
Hpy188I TCNGA 4 cut(s) 80, 498, 538, 1226
Hpy188III TCNNGA 5 cut(s) 16, 600, 686, 1099, 1447
Hpy8I GTNNAC 7 cut(s) 441, 527, 666, 847, 1074, 1177, 1199
Hpy99I CGWCG 1 cut(s) 851
HpyAV CCTTC 4 cut(s) 61, 85, 295, 1190
HpyCH4III ACNGT 2 cut(s) 167, 1593
HpyCH4IV ACGT 1 cut(s) 1443
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 470, 563, 956, 998, 1313
HpyF3I CTNAG 1 cut(s) 1430
HpySE526I ACGT 1 cut(s) 1443
HspAI GCGC 1 cut(s) 864
Kzo9I GATC 2 cut(s) 183, 721
LguI GCTCTTC 1 cut(s) 1183
LmnI GCTCC 2 cut(s) 1274, 1502
Lsp1109I GCAGC 5 cut(s) 280, 702, 715, 873, 1303
LweI GCATC 5 cut(s) 319, 794, 954, 1378, 1396
MaeI CTAG 1 cut(s) 251
MaeII ACGT 1 cut(s) 1443
MaeIII GTNAC 2 cut(s) 898, 1082
MalI GATC 2 cut(s) 185, 723
MboI GATC 2 cut(s) 183, 721
MboII GAAGA 7 cut(s) 615, 758, 1200, 1358, 1397, 1586, 1589
MhlI GDGCHC 1 cut(s) 459
MlsI TGGCCA 1 cut(s) 1373
MluCI AATT 5 cut(s) 57, 178, 646, 886, 1348
MluNI TGGCCA 1 cut(s) 1373
MlyI GAGTC 4 cut(s) 128, 522, 527, 1037
MmeI TCCRAC 4 cut(s) 235, 476, 623, 781
Mox20I TGGCCA 1 cut(s) 1373
Mph1103I ATGCAT 1 cut(s) 984
MroXI GAANNNNTTC 2 cut(s) 305, 1465
MscI TGGCCA 1 cut(s) 1373
MseI TTAA 1 cut(s) 56
MslI CAYNNNNRTG 6 cut(s) 510, 755, 875, 987, 993, 1235
Msp20I TGGCCA 1 cut(s) 1373
MspI CCGG 3 cut(s) 770, 1043, 1458
MspR9I CCNGG 3 cut(s) 624, 1043, 1208
Mva1269I GAATGC 5 cut(s) 389, 414, 559, 762, 984
MvaI CCWGG 2 cut(s) 624, 1208
MvnI CGCG 1 cut(s) 897
MwoI GCNNNNNNNGC 6 cut(s) 44, 470, 563, 956, 998, 1313
NciI CCSGG 1 cut(s) 1043
NcoI CCATGG 1 cut(s) 1236
NdeII GATC 2 cut(s) 183, 721
NlaIV GGNNCC 4 cut(s) 72, 375, 1264, 1341
NmuCI GTSAC 1 cut(s) 1082
NsiI ATGCAT 1 cut(s) 984
NspI RCATGY 4 cut(s) 515, 735, 992, 1381
OliI CACNNNNGTG 1 cut(s) 755
PaeI GCATGC 2 cut(s) 735, 992
PciI ACATGT 1 cut(s) 511
PciSI GCTCTTC 1 cut(s) 1183
PctI GAATGC 5 cut(s) 389, 414, 559, 762, 984
PdmI GAANNNNTTC 2 cut(s) 305, 1465
PfeI GAWTC 4 cut(s) 278, 632, 811, 1514
PkrI GCNGC 7 cut(s) 9, 295, 717, 730, 863, 896, 1318
PleI GAGTC 4 cut(s) 128, 522, 527, 1036
PpsI GAGTC 4 cut(s) 128, 522, 527, 1036
PpuMI RGGWCCY 1 cut(s) 374
PscI ACATGT 1 cut(s) 511
PsiI TTATAA 1 cut(s) 827
Psp5II RGGWCCY 1 cut(s) 374
Psp6I CCWGG 2 cut(s) 622, 1206
PspGI CCWGG 2 cut(s) 622, 1206
PspN4I GGNNCC 4 cut(s) 72, 375, 1264, 1341
PspPI GGNCC 3 cut(s) 374, 1263, 1339
PspPPI RGGWCCY 1 cut(s) 374
PstI CTGCAG 1 cut(s) 1321
PstNI CAGNNNCTG 1 cut(s) 1558
RsaI GTAC 2 cut(s) 440, 881
RsaNI GTAC 2 cut(s) 439, 880
RseI CAYNNNNRTG 6 cut(s) 510, 755, 875, 987, 993, 1235
SalI GTCGAC 2 cut(s) 845, 1175
SapI GCTCTTC 1 cut(s) 1183
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 7 cut(s) 8, 294, 716, 729, 862, 895, 1317
Sau3AI GATC 2 cut(s) 183, 721
Sau96I GGNCC 3 cut(s) 374, 1263, 1339
SchI GAGTC 4 cut(s) 128, 522, 527, 1037
ScrFI CCNGG 3 cut(s) 624, 1043, 1208
SduI GDGCHC 1 cut(s) 459
SfaNI GCATC 5 cut(s) 319, 794, 954, 1378, 1396
SfcI CTRYAG 1 cut(s) 1317
SinI GGWCC 2 cut(s) 374, 1339
SmiMI CAYNNNNRTG 6 cut(s) 510, 755, 875, 987, 993, 1235
SmlI CTYRAG 3 cut(s) 14, 320, 458
SmoI CTYRAG 3 cut(s) 14, 320, 458
SphI GCATGC 2 cut(s) 735, 992
Sse9I AATT 5 cut(s) 57, 178, 646, 886, 1348
SsiI CCGC 4 cut(s) 412, 895, 967, 1211
SspMI CTAG 1 cut(s) 251
StyD4I CCNGG 3 cut(s) 622, 1041, 1206
StyI CCWWGG 1 cut(s) 1236
TaaI ACNGT 2 cut(s) 167, 1593
TaiI ACGT 1 cut(s) 1446
TaqI TCGA 8 cut(s) 182, 309, 531, 599, 846, 1176, 1215, 1473
TasI AATT 5 cut(s) 57, 178, 646, 886, 1348
TauI GCSGC 1 cut(s) 897
TfiI GAWTC 4 cut(s) 278, 632, 811, 1514
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 5 cut(s) 170, 217, 280, 910, 1590
TseFI GTSAC 1 cut(s) 1082
TseI GCWGC 6 cut(s) 7, 293, 715, 728, 861, 1316
Tsp45I GTSAC 1 cut(s) 1082
TspDTI ATGAA 9 cut(s) 51, 85, 113, 494, 630, 803, 854, 1084, 1398
TspGWI ACGGA 1 cut(s) 313
TspRI CASTG 5 cut(s) 170, 217, 280, 910, 1590
VpaK11BI GGWCC 2 cut(s) 374, 1339
XapI RAATTY 2 cut(s) 646, 1348
XceI RCATGY 4 cut(s) 515, 735, 992, 1381
XcmI CCANNNNNNNNNTGG 1 cut(s) 209
XmiI GTMKAC 3 cut(s) 665, 846, 1176
XmnI GAANNNNTTC 2 cut(s) 305, 1465
XspI CTAG 1 cut(s) 251
Zsp2I ATGCAT 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.