Rw5G014790

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014691
Physical Location & Seq
Reverse (-)
17739033 .. 17740631
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G014790.1

Sequence Viewer

Length: 1599 bp
ATGTTGCCGCTACAGAAGACCCAAATGAATGGGTTAGCTAGACAAGCAGAGCTTTTAGTTTCACGAACCGAAAGCTTTAATTTATTGACGTTTTTCGTTAGGGGTAGAAGAACATACATGAAACCCCATTACAGAAACTCCTATGATTACACAGACTTGCTTCAACATTGCAGGAACACCAAATCAATCAAGAAACTCCACGCCCAGATCATCATCGGAGGTCTACACCAAAACCCATTTGTGGCTACAAAGCTAGTAGGCAAGTACGTGGAGTGTGGTGACTCAAGTATGGGAGATGCACGGAAGGTGTTCGATGAATTGACTGAGAGAGATGTGTTTGTGTGGAACATGGTCATTCAGGGTTATGCAAATGTTGGTCCTTTTGCTGAAGCCCTTAAAATGTGTAATAGAATGAGGCTGAGTGGGTTATCCCCAAATCGGTATACTTACCCTTTTGTGCTTAAGGCTTGTGGAGCAATGAGAGATGGAAAGCAGGGTCAGGTTGTTCATGGGCAGATTGTGAAATCTGGGCTTGACTTGCAGTTGTTTGTGGGGAATGCTCTTGTGGCATTGTATTCCAAGTGTGCGGAAGTTGAGGTATCGAGAAGAGTGTTCGATGAATTGCCTCTGAAAGATCTTGTTAGTTGGAATTCCATGATTTCGGGGTATGCCACAAATGGATACCCGAATGAGGCTGTTGAAATTTTTCGTGCAATGATACAAGGTCCTACTACATGCTTGCCTGACCATGCCACTCTGGTTTGCGTGCTTCCGGCTTGTATTGAAGCATCTGCTATTGAAGTTGGGTTTTGGATTCATTCTTACATTGTGAAGACAAGTATAAAAGTTGACTCTGCTTTGGGCAGTGCTCTCATTTCAATGTATGCAAACTGTGGTCGCGTAAGAGTTTCCAGAGTTATCTTTGACCGAGTAAGTGATAAGAATGTGGTGCTGTGGAGCGCCATGATGAGGTGTTATGGAATGCATGGGCACACAGACGAGGTGCTCCAGATGTTTTTGCAGTTTGAGGAGTCCGGTTTATACCCAGATGCTGTTGTGGTTTTGTGTTTATTGTCCACTTGTAGTCACGCAGGGATGGTTTCAAAAGGCTTGGAAATTTTTGAAAAAATGGAAGATTACGGAGTAGAGAAAAACGAGAAGCATTATGCTTGCATTGTAGACCTTCTGGGAAGAGCTGGGTTAATTGACCGGGCAGTCAAGTTTATTGAAAGCATGCCCATACAGGCAGGGAAAGATGTATACGGTGCACTACTTGGAGCATGTAGGATACATAATAACATAGAACTTGCTGAAGAAACTGCAGAGAAATTGTTTGTTTTGGATCCTGGAAATGCTGGGAGGTATATTCTTCTGGCCAGCATGTATGAAGATGCAGGGCGATGGGAAGATGCAGCTAGAGTGAGGAGGCTAATCAGAGAGAAGAACATCAAGAAGCCAACTGGACGTAGTTCGATTGAGGTGGATTGCATCTATCACACATTTGGAGCTGATGATGAGTCTCACCCATATACAGACCAGATATTTGACACATTGGAGAGAATGGATAGGATAATGGAAGAAGCCATAGTAATCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

532

Amino Acids

59.76

Weight (kDa)

6.55

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 110 - 157 1.3e-10 PPR repeat family
PPR_3 PF13812 110 - 159 8.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 212 - 260 2.3e-06 PPR repeat family
PPR PF01535 214 - 240 1.3e-07 PPR repeat
E_motif PF20431 431 - 493 5.3e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014691)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g13960
malus_domestica MD05G1239500.v1.1 MD10G1217700.v1.1
prunus_persica Prupe.4G126300_v2.0.a1
pyrus_communis pycom05g21580 pycom10g18360
rosa_chinensis RchiOBHm_Chr5g0023171
rosa_laevigata RLG00000032705
rosa_multiflora Rmu_sc0007300.1_g000014
rosa_roxburghii Rroxscaffold_1G00056170
rosa_rugosa Rorug05G0077700
rosa_samantha Rh5AG163900 Rh5BG163200 Rh5CG178200 Rh5DG164600
rosa_wichuraiana Rw0G018300 Rw5G014790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1214
AccI GTMKAC 4 cut(s) 223, 443, 1179, 1260
AccII CGCG 1 cut(s) 900
AciI CCGC 2 cut(s) 8, 587
AclWI GGATC 2 cut(s) 1337, 1350
AcoI YGGCCR 1 cut(s) 1374
AcsI RAATTY 3 cut(s) 649, 702, 1116
AcuI CTGAAG 2 cut(s) 408, 1332
AfaI GTAC 1 cut(s) 266
AfiI CCNNNNNNNGG 4 cut(s) 241, 438, 691, 969
AflII CTTAAG 1 cut(s) 461
AgsI TTSAA 8 cut(s) 164, 701, 785, 800, 879, 1104, 1124, 1229
AjnI CCWGG 1 cut(s) 1345
AluBI AGCT 7 cut(s) 38, 52, 75, 253, 1196, 1415, 1508
AluI AGCT 7 cut(s) 38, 52, 75, 253, 1196, 1415, 1508
Alw21I GWGCWC 3 cut(s) 871, 1008, 1270
Alw26I GTCTC 1 cut(s) 1524
Alw44I GTGCAC 1 cut(s) 1266
AlwI GGATC 2 cut(s) 1337, 1350
AlwNI CAGNNNCTG 1 cut(s) 1052
AoxI GGCC 1 cut(s) 1374
ApaLI GTGCAC 1 cut(s) 1266
ApeKI GCWGC 1 cut(s) 1412
ApoI RAATTY 3 cut(s) 649, 702, 1116
ArsI GACNNNNNNTTYG 2 cut(s) 1526, 1558
Asp700I GAANNNNTTC 2 cut(s) 308, 705
AspLEI GCGC 1 cut(s) 962
AspS9I GGNCC 2 cut(s) 377, 725
AsuC2I CCSGG 1 cut(s) 1211
AsuHPI GGTGA 2 cut(s) 290, 1514
AvaII GGWCC 2 cut(s) 377, 725
BaeGI GKGCMC 2 cut(s) 993, 1270
BalI TGGCCA 1 cut(s) 1376
BamHI GGATCC 1 cut(s) 1342
BbsI GAAGAC 2 cut(s) 23, 839
Bbv12I GWGCWC 3 cut(s) 871, 1008, 1270
BbvI GCAGC 1 cut(s) 1424
BccI CCATC 3 cut(s) 479, 1090, 1395
BciT130I CCWGG 1 cut(s) 1347
BciVI GTATCC 2 cut(s) 674, 1281
BcnI CCSGG 1 cut(s) 1211
BcoDI GTCTC 1 cut(s) 1524
BfaI CTAG 3 cut(s) 39, 254, 1416
BfmI CTRYAG 2 cut(s) 11, 1320
BfoI RGCGCY 1 cut(s) 963
BfrI CTTAAG 1 cut(s) 461
BfuI GTATCC 2 cut(s) 674, 1281
BglII AGATCT 1 cut(s) 634
BisI GCNGC 2 cut(s) 8, 1413
BlsI GCNGC 2 cut(s) 9, 1414
Bme1390I CCNGG 2 cut(s) 1211, 1347
Bme18I GGWCC 2 cut(s) 377, 725
BmgT120I GGNCC 2 cut(s) 377, 725
BmiI GGNNCC 1 cut(s) 1344
BmrFI CCNGG 2 cut(s) 1211, 1347
BmsI GCATC 6 cut(s) 286, 797, 1039, 1381, 1399, 1497
BpiI GAAGAC 2 cut(s) 23, 839
BpmI CTGGAG 1 cut(s) 992
BpuEI CTTGAG 1 cut(s) 268
BpuMI CCSGG 1 cut(s) 1211
BsaAI YACGTR 1 cut(s) 268
BsaBI GATNNNNATC 1 cut(s) 212
BsaWI WCCGGW 1 cut(s) 1034
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 438, 691, 969
Bse1I ACTGG 1 cut(s) 1465
Bse3DI GCAATG 3 cut(s) 166, 483, 720
Bse8I GATNNNNATC 1 cut(s) 212
BseBI CCWGG 1 cut(s) 1347
BseGI GGATG 1 cut(s) 1101
BseJI GATNNNNATC 1 cut(s) 212
BseLI CCNNNNNNNGG 4 cut(s) 241, 438, 691, 969
BseMI GCAATG 3 cut(s) 166, 483, 720
BseMII CTCAG 2 cut(s) 315, 410
BseNI ACTGG 1 cut(s) 1465
BseRI GAGGAG 2 cut(s) 1043, 1438
BseSI GKGCMC 2 cut(s) 993, 1270
BseXI GCAGC 1 cut(s) 1424
BseYI CCCAGC 2 cut(s) 1196, 1355
Bsh1236I CGCG 1 cut(s) 900
BshFI GGCC 1 cut(s) 1376
BsiHKAI GWGCWC 3 cut(s) 871, 1008, 1270
BsiSI CCGG 3 cut(s) 773, 1035, 1210
BslI CCNNNNNNNGG 4 cut(s) 241, 438, 691, 969
BsmAI GTCTC 1 cut(s) 1524
BsmI GAATGC 2 cut(s) 562, 987
BsnI GGCC 1 cut(s) 1376
Bsp1286I GDGCHC 4 cut(s) 871, 993, 1008, 1270
Bsp143I GATC 3 cut(s) 207, 634, 1342
BspACI CCGC 2 cut(s) 8, 587
BspANI GGCC 1 cut(s) 1376
BspCNI CTCAG 2 cut(s) 316, 411
BspFNI CGCG 1 cut(s) 900
BspLI GGNNCC 1 cut(s) 1344
BspMAI CTGCAG 1 cut(s) 1324
BspPI GGATC 2 cut(s) 1337, 1350
BspQI GCTCTTC 1 cut(s) 1186
BspTI CTTAAG 1 cut(s) 461
BsrDI GCAATG 3 cut(s) 166, 483, 720
BsrI ACTGG 1 cut(s) 1465
BssMI GATC 3 cut(s) 207, 634, 1342
BssNAI GTATAC 2 cut(s) 444, 1261
Bst1107I GTATAC 2 cut(s) 444, 1261
Bst2UI CCWGG 1 cut(s) 1347
Bst4CI ACNGT 2 cut(s) 893, 1265
Bst6I CTCTTC 2 cut(s) 601, 1186
BstAFI CTTAAG 1 cut(s) 461
BstBAI YACGTR 1 cut(s) 268
BstC8I GCNNGC 5 cut(s) 740, 767, 1171, 1235, 1378
BstDEI CTNAG 2 cut(s) 324, 419
BstF5I GGATG 1 cut(s) 1101
BstFNI CGCG 1 cut(s) 900
BstH2I RGCGCY 1 cut(s) 963
BstHHI GCGC 1 cut(s) 962
BstKTI GATC 3 cut(s) 210, 637, 1345
BstMAI GTCTC 1 cut(s) 1524
BstMBI GATC 3 cut(s) 207, 634, 1342
BstMWI GCNNNNNNNGC 4 cut(s) 44, 473, 538, 566
BstNI CCWGG 1 cut(s) 1347
BstNSI RCATGY 4 cut(s) 738, 1237, 1284, 1384
BstSCI CCNGG 2 cut(s) 1209, 1345
BstSFI CTRYAG 2 cut(s) 11, 1320
BstSLI GKGCMC 2 cut(s) 993, 1270
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 1 cut(s) 1424
BstV2I GAAGAC 2 cut(s) 23, 839
BstX2I RGATCY 2 cut(s) 634, 1342
BstXI CCANNNNNNTGG 1 cut(s) 29
BstYI RGATCY 2 cut(s) 634, 1342
BstZ17I GTATAC 2 cut(s) 444, 1261
BsuI GTATCC 2 cut(s) 674, 1281
BsuRI GGCC 1 cut(s) 1376
BtgZI GCGATG 1 cut(s) 1414
BtsCI GGATG 1 cut(s) 1101
BtsI GCAGTG 1 cut(s) 871
BtsIMutI CAGTG 1 cut(s) 871
Cac8I GCNNGC 5 cut(s) 740, 767, 1171, 1235, 1378
CaiI CAGNNNCTG 1 cut(s) 1052
CfoI GCGC 1 cut(s) 962
Cfr13I GGNCC 2 cut(s) 377, 725
Csp6I GTAC 1 cut(s) 265
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 2 cut(s) 324, 419
DpnI GATC 3 cut(s) 209, 636, 1344
DpnII GATC 3 cut(s) 207, 634, 1342
DrdI GACNNNNNNGTC 1 cut(s) 1214
DseDI GACNNNNNNGTC 1 cut(s) 1214
EaeI YGGCCR 1 cut(s) 1374
Eam1104I CTCTTC 2 cut(s) 601, 1186
EarI CTCTTC 2 cut(s) 601, 1186
Eco47I GGWCC 2 cut(s) 377, 725
Eco57I CTGAAG 2 cut(s) 408, 1332
EcoO109I RGGNCCY 1 cut(s) 725
EcoRI GAATTC 1 cut(s) 649
EcoRII CCWGG 1 cut(s) 1345
EcoT22I ATGCAT 1 cut(s) 987
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FblI GTMKAC 4 cut(s) 223, 443, 1179, 1260
Fnu4HI GCNGC 2 cut(s) 8, 1413
FokI GGATG 1 cut(s) 1108
Fsp4HI GCNGC 2 cut(s) 8, 1413
FspBI CTAG 3 cut(s) 39, 254, 1416
GlaI GCGC 1 cut(s) 961
GluI GCNGC 2 cut(s) 8, 1413
GsaI CCCAGC 2 cut(s) 1200, 1359
GsuI CTGGAG 1 cut(s) 992
HaeII RGCGCY 1 cut(s) 963
HaeIII GGCC 1 cut(s) 1376
HapII CCGG 3 cut(s) 773, 1035, 1210
HhaI GCGC 1 cut(s) 962
Hin6I GCGC 1 cut(s) 960
HinP1I GCGC 1 cut(s) 960
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 1 cut(s) 73
HinfI GANTC 5 cut(s) 281, 814, 851, 1031, 1517
HpaII CCGG 3 cut(s) 773, 1035, 1210
HphI GGTGA 2 cut(s) 290, 1514
Hpy166II GTNNAC 7 cut(s) 224, 444, 850, 1077, 1180, 1261, 1268
Hpy188I TCNGA 3 cut(s) 218, 630, 1436
Hpy188III TCNNGA 6 cut(s) 63, 190, 603, 912, 1009, 1450
Hpy8I GTNNAC 7 cut(s) 224, 444, 850, 1077, 1180, 1261, 1268
HpyAV CCTTC 2 cut(s) 298, 1193
HpyCH4III ACNGT 2 cut(s) 893, 1265
HpyCH4IV ACGT 3 cut(s) 89, 267, 1465
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 473, 538, 566
HpyF3I CTNAG 2 cut(s) 324, 419
HpySE526I ACGT 3 cut(s) 89, 267, 1465
HspAI GCGC 1 cut(s) 960
Kzo9I GATC 3 cut(s) 207, 634, 1342
LguI GCTCTTC 1 cut(s) 1186
LmnI GCTCC 5 cut(s) 473, 957, 1011, 1277, 1505
Lsp1109I GCAGC 1 cut(s) 1424
LweI GCATC 6 cut(s) 286, 797, 1039, 1381, 1399, 1497
MaeI CTAG 3 cut(s) 39, 254, 1416
MaeII ACGT 3 cut(s) 89, 267, 1465
MaeIII GTNAC 2 cut(s) 278, 1085
MalI GATC 3 cut(s) 209, 636, 1344
MboI GATC 3 cut(s) 207, 634, 1342
MflI RGATCY 2 cut(s) 634, 1342
MhlI GDGCHC 4 cut(s) 871, 993, 1008, 1270
MlsI TGGCCA 1 cut(s) 1376
MluCI AATT 8 cut(s) 79, 317, 620, 649, 702, 1116, 1203, 1328
MluNI TGGCCA 1 cut(s) 1376
MlyI GAGTC 4 cut(s) 275, 845, 1040, 1526
MmeI TCCRAC 1 cut(s) 626
Mox20I TGGCCA 1 cut(s) 1376
Mph1103I ATGCAT 1 cut(s) 987
MroXI GAANNNNTTC 2 cut(s) 308, 705
MscI TGGCCA 1 cut(s) 1376
MseI TTAA 4 cut(s) 78, 396, 462, 1202
MslI CAYNNNNRTG 1 cut(s) 878
Msp20I TGGCCA 1 cut(s) 1376
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 3 cut(s) 773, 1035, 1210
MspR9I CCNGG 2 cut(s) 1211, 1347
Mva1269I GAATGC 2 cut(s) 562, 987
MvaI CCWGG 1 cut(s) 1347
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 4 cut(s) 44, 473, 538, 566
NciI CCSGG 1 cut(s) 1211
NdeII GATC 3 cut(s) 207, 634, 1342
NlaIV GGNNCC 1 cut(s) 1344
NmuCI GTSAC 2 cut(s) 278, 1085
NsiI ATGCAT 1 cut(s) 987
NspI RCATGY 4 cut(s) 738, 1237, 1284, 1384
PaeI GCATGC 1 cut(s) 1237
PciSI GCTCTTC 1 cut(s) 1186
PctI GAATGC 2 cut(s) 562, 987
PdmI GAANNNNTTC 2 cut(s) 308, 705
PfeI GAWTC 1 cut(s) 814
PfoI TCCNGGA 1 cut(s) 1345
PkrI GCNGC 2 cut(s) 9, 1414
PleI GAGTC 4 cut(s) 275, 845, 1039, 1525
PpsI GAGTC 4 cut(s) 275, 845, 1039, 1525
Ppu21I YACGTR 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 725
Psp5II RGGWCCY 1 cut(s) 725
Psp6I CCWGG 1 cut(s) 1345
PspFI CCCAGC 2 cut(s) 1196, 1355
PspGI CCWGG 1 cut(s) 1345
PspN4I GGNNCC 1 cut(s) 1344
PspPI GGNCC 2 cut(s) 377, 725
PspPPI RGGWCCY 1 cut(s) 725
PstI CTGCAG 1 cut(s) 1324
PstNI CAGNNNCTG 1 cut(s) 1052
PsuI RGATCY 2 cut(s) 634, 1342
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
RseI CAYNNNNRTG 1 cut(s) 878
SapI GCTCTTC 1 cut(s) 1186
SaqAI TTAA 4 cut(s) 78, 396, 462, 1202
SatI GCNGC 2 cut(s) 8, 1413
Sau3AI GATC 3 cut(s) 207, 634, 1342
Sau96I GGNCC 2 cut(s) 377, 725
SchI GAGTC 4 cut(s) 275, 845, 1040, 1526
ScrFI CCNGG 2 cut(s) 1211, 1347
SduI GDGCHC 4 cut(s) 871, 993, 1008, 1270
SfaNI GCATC 6 cut(s) 286, 797, 1039, 1381, 1399, 1497
SfcI CTRYAG 2 cut(s) 11, 1320
SinI GGWCC 2 cut(s) 377, 725
SmiMI CAYNNNNRTG 1 cut(s) 878
SmlI CTYRAG 2 cut(s) 283, 461
SmoI CTYRAG 2 cut(s) 283, 461
SphI GCATGC 1 cut(s) 1237
Sse9I AATT 8 cut(s) 79, 317, 620, 649, 702, 1116, 1203, 1328
SsiI CCGC 2 cut(s) 8, 587
SspMI CTAG 3 cut(s) 39, 254, 1416
StyD4I CCNGG 2 cut(s) 1209, 1345
TaaI ACNGT 2 cut(s) 893, 1265
TaiI ACGT 3 cut(s) 92, 270, 1468
TaqI TCGA 4 cut(s) 312, 602, 615, 1472
TaqII GACCGA 1 cut(s) 942
TasI AATT 8 cut(s) 79, 317, 620, 649, 702, 1116, 1203, 1328
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 1 cut(s) 814
Tru1I TTAA 4 cut(s) 78, 396, 462, 1202
Tru9I TTAA 4 cut(s) 78, 396, 462, 1202
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 2 cut(s) 278, 1085
TseI GCWGC 1 cut(s) 1412
Tsp45I GTSAC 2 cut(s) 278, 1085
TspDTI ATGAA 7 cut(s) 41, 134, 330, 497, 633, 806, 1401
TspGWI ACGGA 2 cut(s) 316, 1155
TspRI CASTG 1 cut(s) 871
Vha464I CTTAAG 1 cut(s) 461
VneI GTGCAC 1 cut(s) 1266
VpaK11BI GGWCC 2 cut(s) 377, 725
XapI RAATTY 3 cut(s) 649, 702, 1116
XceI RCATGY 4 cut(s) 738, 1237, 1284, 1384
XmiI GTMKAC 4 cut(s) 223, 443, 1179, 1260
XmnI GAANNNNTTC 2 cut(s) 308, 705
XspI CTAG 3 cut(s) 39, 254, 1416
Zsp2I ATGCAT 1 cut(s) 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.