pycom05g23530

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006392
Physical Location & Seq
Reverse (-)
25401354 .. 25404968
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2955 bp
ATGGCTTCCTTGAAGGAAATGCTCACTGAAGAAAGACTCGAACTCGAAAGCAAAAGGTACCCGAAAGGCCAGAAACCATTCAAGTACAGAGAAATATATGAATCTATGGCTCTTTTGCTGCCTATACACATCTGCCATGATCGGAAAAGTGGCGATTTTTCGGACCACAAGGCTCCGACGCGAAAAGGGTTGTCGAGAAGGGTTAATACTACTTCGGAGAGGTCGAATTCCAAGTCATTGGTTTCGGAGAGTTCGAGGACTAGTGAGCCTGCTGCCATTGATGAAGTTGCCACTGGAGCTGTGGTTTCTATACTTAGTGGTTACGTAGGGAGGTACGTGAAGGATGAGGCTTTTCAGGAAACAATCCGTGAAAAGTGCAGGTCTTGCTTGGTGAGGAAGAAAAAGGATTTGGATAATGGGATGTTGGGAAACTTGGAATCGGGTATTGAAAGTGTTGAGAAGTTGGTGGAAGATCAATGGTCGACGAAGAAGGAAGTTAGGGCGAAAACAGTGAAGAATTTGATTCGGGTTTTTAACGTTGTTGGTTCCTCAAATTCTAGCAATTCTCATATTTCTGCTTGTGCTCAGCTTTACTTGTCAATTGTTCACAAGCTTGAGAAAAATGACCAGCTTTCGTCATGGCACTTGCTTCAAGTGTTCTGTGATTCACCGGGTTTGGCACGCACACATTTGCTCCCTGATCTTTGGGAGCATTTGTTTCTTCCACATCTTTTACATGTGAAGGTTTGGTATGCCAAGGAAATGGATGCTCTTTCGAGCTCAGAGGACGGTGAGAAGGAGAAGAAAATGAAAGCCATGACGAGCGTGTATGATGAACAAATGGATATGGGAACAAAAAAGTTTGCTTTGTACTACAAGGAGTGGCTTCAAGTGGGGGCTGAGGCTCCTCCCATTCCTCCTACTATACCTTTACCTTCTTCGATAAGCAGTTGCAGATCATCGAGAAGGAGATCATCAGATTCTTACACTTCACATTCCTCACTCAACAAAAATTTATACCGATCTGTGTTTGGCTCCACGCTTGAGCGAAGATCTGTGAACCTCGATGATGGAAAAGGAGTTTTAAATGATACTTGGGGTTTGGATGAAGAGGAGGAAAATCTTTGTGCAGATTATGTTCATAGGGGAGAAAGGTCTAGTTGGCGAAGGTCATCAAGCGAAAGTCATAGAAATCCGATAATTGCGCCATGGCCTGAGTCAGAGACACAGAAGTCAGACCATTTTGGATTCTTTAGATGCCAGAATGCACCAACAGTATGCTTGGTGAGCAAGAATCTCATAGTCAAGAACAATTCAATCCGAAAAGAAGATAACTCCCATCTTCGACCTTCAAGCAACTTAAGCCGTGCTATTAGCACTATCTGCTCCTCCGATAATCCAAGTGATTGCGAAACTGCAATTCGTGTGATTACCAAGGCTTGGTTGGACTCACATGGTGATCCTGTCGTTGAGGCTGAGCTATCAAAGCCACCCGTTATTCAGGGAATGTTGGAGGTGTTGTTTGCTTCTAGTGATGAAGAAATTTTGGAATTAGTAATCTCAGTTTTAGCTGAACTCACAACGAGAAACGAGATGATTAGACAGATCATACTGAACTCAGATCCACAGCTTGAAATTTTTATGAGACTTCTAAGAAGTAATGGTCTATTTTTGAAAGCTGCAATTCTGCTTTACTTGTTGAAGCCAAAGGCAAAACAGATGATATCAGTCGAATGGGCAGCGCTAGTCCTTCGAGTACTAGAGTTTGGAGATCAGTTGCAGATGCTATTCACAGTTCGTTGCAGTCCTCAAGTGGCGGCATTGTACCTTTTAGACCAGCTTCTTACCGGTTTTGATGAAGATCGAAACTTGGAGAATGCTGGAGAGGTAATTTCTCTAGGAGGGCTGAGCCTTCTGGTGGCACAAATCGAGAGAGGGGAGACTCATGAGAGGAACAACATTACTTCGATCATTGTGTGTTGTGTTCGTGCTGATGGTAGGTGTCGAAACTACTTGGCTGATTTTCTCAACAAGGCTTCTCTTCTTGAACTCGTTGTACTTGGAAATGGCAAGAACTCTACTGGCTCCGCATTCACCCTATTGATCGAAATGCTCTGCCTTAGTAGACGAACAAAGATCACTGAAATCCTAGATGGGCTGAAAGAAGGATCGGGCGGCTTTAACACAATGCAGATTTTATTGGTCTATCTGCAGAGAGCTCCACTTGAAGAACGCCCGTTAATTGCATCAGTATTGCTACAGCTTGATCTTATGGGAGACCCTTTCAGGAGCAGTGTATACAGAGAAGAAGCCATTGAAGCAATCATAGAAGCTATAAACTGTCGAACGTGCCACGTAAAAGTCCAGCAACGATCAGCAAGAGCTCTCTCAATGTTGGGAGGCCGGTTTTCTGATGCCGGCGAGGCAACAACAGAACATTGGCTTTTACAAGAAGCAGGTTACAGTCATTGGCCAGGAAATTCATTTCTCTTTAAAGAAAACATTGTGGATGGTTTTGCACACTCAAACGAAGACGAAGCAGCAACGGAAAATTGGCAAAGAAAAACAGCCACTGTTTTGTTCAAAAGTGGCAACAAGGAACTACTCGTGGCCCTTTCGGATTCCATGGCGAATGGCATCCCAAGTTTAGCACGATCGAGCCTTGTGACGGTGTCATGGATGAGTAGATTTCTCGATGCAGTCGGAGGTGAAGATTTGAGGTCAATGACATGGTCAGTTCTTGTGCCTCAGCTGCTAGAATATCTGAATTATGACAGAGATGTGGAGGAAAGAGTGCTTGCTTCATACACACTGCTCAAATTAGCAAAAAGTTCAGCTCATGAATATGTCCCCATGCTATCATCAGCGGACAAAGATTTGCTCAGGAAACTTCAGAACCTCTCCTTTGTGACATGGACAGCCAATGAACTTATCTCAATCATCACAAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

985

Amino Acids

110.52

Weight (kDa)

6.13

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 96 - 313 6.4e-90 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 451 - 612 4.4e-60 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 613 - 981 2.2e-129 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1231
Acc36I ACCTGC 2 cut(s) 369, 2447
Acc65I GGTACC 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 57
AccB7I CCANNNNNTGG 1 cut(s) 1440
AccI GTMKAC 3 cut(s) 482, 2125, 2298
AccII CGCG 1 cut(s) 181
AciI CCGC 4 cut(s) 1817, 2088, 2175, 2867
AclI AACGTT 1 cut(s) 537
AclWI GGATC 3 cut(s) 1454, 1616, 2176
AcoI YGGCCR 1 cut(s) 2471
AcsI RAATTY 7 cut(s) 226, 517, 553, 1012, 1542, 1635, 2479
AcuI CTGAAG 2 cut(s) 48, 2876
AdeI CACNNNGTG 1 cut(s) 1457
AfaI GTAC 7 cut(s) 59, 86, 335, 872, 1758, 1826, 2058
AfeI AGCGCT 1 cut(s) 1743
AfiI CCNNNNNNNGG 3 cut(s) 1440, 1918, 2668
AflII CTTAAG 1 cut(s) 1360
AflIII ACRYGT 1 cut(s) 736
AgeI ACCGGT 1 cut(s) 1847
AhlI ACTAGT 1 cut(s) 260
AjnI CCWGG 1 cut(s) 2473
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
Alw21I GWGCWC 4 cut(s) 586, 782, 2221, 2386
Alw26I GTCTC 4 cut(s) 1217, 1639, 1934, 2271
AlwI GGATC 3 cut(s) 1454, 1616, 2176
AlwNI CAGNNNCTG 1 cut(s) 2573
Aor51HI AGCGCT 1 cut(s) 1743
AoxI GGCC 5 cut(s) 67, 1211, 2401, 2471, 2610
ApeKI GCWGC 6 cut(s) 118, 272, 1679, 1739, 2540, 2752
ApoI RAATTY 7 cut(s) 226, 517, 553, 1012, 1542, 1635, 2479
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AsiGI ACCGGT 1 cut(s) 1847
Asp700I GAANNNNTTC 1 cut(s) 77
Asp718I GGTACC 1 cut(s) 57
AspLEI GCGC 2 cut(s) 1207, 1744
AspS9I GGNCC 2 cut(s) 163, 2611
AsuC2I CCSGG 1 cut(s) 672
AsuHPI GGTGA 7 cut(s) 403, 660, 803, 1297, 1469, 2086, 2720
AvaII GGWCC 1 cut(s) 163
BalI TGGCCA 1 cut(s) 2473
BanI GGYRCC 1 cut(s) 57
BanII GRGCYC 3 cut(s) 782, 2221, 2386
BarI GAAGNNNNNNTAC 2 cut(s) 190, 222
BauI CACGAG 1 cut(s) 2606
BbsI GAAGAC 1 cut(s) 2538
Bbv12I GWGCWC 4 cut(s) 586, 782, 2221, 2386
BbvCI CCTCAGC 2 cut(s) 900, 2748
BbvI GCAGC 6 cut(s) 105, 259, 1666, 1751, 2552, 2739
BccI CCATC 5 cut(s) 1064, 1347, 1988, 2147, 2504
BceAI ACGGC 1 cut(s) 1350
BciT130I CCWGG 1 cut(s) 2475
BcnI CCSGG 1 cut(s) 672
BcoDI GTCTC 4 cut(s) 1217, 1639, 1934, 2271
BcuI ACTAGT 1 cut(s) 260
BfaI CTAG 9 cut(s) 261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
BfmI CTRYAG 2 cut(s) 2210, 2258
BfoI RGCGCY 1 cut(s) 1745
BfrI CTTAAG 1 cut(s) 1360
BfuAI ACCTGC 2 cut(s) 369, 2447
BglI GCCNNNNNGGC 1 cut(s) 2423
BglII AGATCT 1 cut(s) 1052
BisI GCNGC 8 cut(s) 119, 273, 1680, 1740, 1818, 2176, 2541, 2753
BlpI GCTNAGC 3 cut(s) 585, 1476, 1907
BlsI GCNGC 8 cut(s) 120, 274, 1681, 1741, 1819, 2177, 2542, 2754
BmcAI AGTACT 1 cut(s) 1758
Bme1390I CCNGG 2 cut(s) 672, 2475
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 2 cut(s) 163, 2611
BmiI GGNNCC 6 cut(s) 59, 174, 547, 906, 1036, 2086
BmrFI CCNGG 2 cut(s) 672, 2475
BmsI GCATC 7 cut(s) 757, 1247, 1773, 2255, 2404, 2646, 2686
BpiI GAAGAC 1 cut(s) 2538
BpmI CTGGAG 2 cut(s) 315, 1902
Bpu10I CCTNAGC 3 cut(s) 900, 2748, 2882
Bpu1102I GCTNAGC 3 cut(s) 585, 1476, 1907
BpuEI CTTGAG 3 cut(s) 635, 1064, 1794
BpuMI CCSGG 1 cut(s) 672
BsaAI YACGTR 3 cut(s) 325, 337, 2356
BsaBI GATNNNNATC 1 cut(s) 1860
BsaI GGTCTC 1 cut(s) 2271
BsaJI CCNNGG 4 cut(s) 756, 1208, 1434, 2625
BsaWI WCCGGW 1 cut(s) 1847
BsaXI ACNNNNNCTCC 8 cut(s) 288, 318, 678, 701, 708, 731, 1505, 1535
Bsc4I CCNNNNNNNGG 3 cut(s) 1440, 1918, 2668
Bse118I RCCGGY 3 cut(s) 1847, 2403, 2417
Bse1I ACTGG 2 cut(s) 298, 2086
Bse8I GATNNNNATC 1 cut(s) 1860
BseBI CCWGG 1 cut(s) 2475
BseDI CCNNGG 4 cut(s) 756, 1208, 1434, 2625
BseGI GGATG 7 cut(s) 349, 426, 772, 1111, 2515, 2637, 2685
BseJI GATNNNNATC 1 cut(s) 1860
BseLI CCNNNNNNNGG 3 cut(s) 1440, 1918, 2668
BseNI ACTGG 2 cut(s) 298, 2086
BseRI GAGGAG 3 cut(s) 897, 1127, 1378
BseXI GCAGC 6 cut(s) 105, 259, 1666, 1751, 2552, 2739
BsgI GTGCAG 2 cut(s) 397, 1149
Bsh1236I CGCG 1 cut(s) 181
Bsh1285I CGRYCG 1 cut(s) 2657
BshFI GGCC 5 cut(s) 69, 1213, 2403, 2473, 2612
BshNI GGYRCC 1 cut(s) 57
BshTI ACCGGT 1 cut(s) 1847
BsiEI CGRYCG 1 cut(s) 2657
BsiHKAI GWGCWC 4 cut(s) 586, 782, 2221, 2386
BsiSI CCGG 4 cut(s) 671, 1848, 2404, 2418
BslFI GGGAC 1 cut(s) 2834
BslI CCNNNNNNNGG 3 cut(s) 1440, 1918, 2668
BsmAI GTCTC 4 cut(s) 1217, 1639, 1934, 2271
BsmFI GGGAC 1 cut(s) 2834
BsmI GAATGC 3 cut(s) 1270, 1882, 2090
BsnI GGCC 5 cut(s) 69, 1213, 2403, 2473, 2612
Bso31I GGTCTC 1 cut(s) 2271
Bsp1286I GDGCHC 4 cut(s) 586, 782, 2221, 2386
Bsp1720I GCTNAGC 3 cut(s) 585, 1476, 1907
Bsp19I CCATGG 2 cut(s) 1208, 2625
BspACI CCGC 4 cut(s) 1817, 2088, 2175, 2867
BspANI GGCC 5 cut(s) 69, 1213, 2403, 2473, 2612
BspFNI CGCG 1 cut(s) 181
BspHI TCATGA 2 cut(s) 1945, 2839
BspLI GGNNCC 6 cut(s) 59, 174, 547, 906, 1036, 2086
BspMAI CTGCAG 1 cut(s) 2214
BspMI ACCTGC 2 cut(s) 369, 2447
BspPI GGATC 3 cut(s) 1454, 1616, 2176
BspT107I GGYRCC 1 cut(s) 57
BspTI CTTAAG 1 cut(s) 1360
BspTNI GGTCTC 1 cut(s) 2271
BsrFI RCCGGY 3 cut(s) 1847, 2403, 2417
BsrI ACTGG 2 cut(s) 298, 2086
BssAI RCCGGY 3 cut(s) 1847, 2403, 2417
BssECI CCNNGG 4 cut(s) 756, 1208, 1434, 2625
BssNAI GTATAC 1 cut(s) 2299
BssSI CACGAG 1 cut(s) 2606
BssT1I CCWWGG 4 cut(s) 756, 1208, 1434, 2625
Bst1107I GTATAC 1 cut(s) 2299
Bst2BI CACGAG 1 cut(s) 2606
Bst2UI CCWGG 1 cut(s) 2475
Bst4CI ACNGT 8 cut(s) 511, 791, 1276, 1795, 2342, 2465, 2575, 2671
Bst6I CTCTTC 2 cut(s) 1104, 2046
BstAFI CTTAAG 1 cut(s) 1360
BstAPI GCANNNNNTGC 2 cut(s) 384, 1383
BstBAI YACGTR 3 cut(s) 325, 337, 2356
BstC8I GCNNGC 4 cut(s) 270, 682, 2419, 2799
BstDSI CCRYGG 2 cut(s) 1208, 2625
BstF5I GGATG 7 cut(s) 349, 426, 772, 1111, 2515, 2637, 2685
BstFNI CGCG 1 cut(s) 181
BstH2I RGCGCY 1 cut(s) 1745
BstHHI GCGC 2 cut(s) 1207, 1744
BstMAI GTCTC 4 cut(s) 1217, 1639, 1934, 2271
BstMCI CGRYCG 1 cut(s) 2657
BstMWI GCNNNNNNNGC 9 cut(s) 296, 384, 1287, 1362, 1383, 1486, 2318, 2423, 2752
BstNI CCWGG 1 cut(s) 2475
BstNSI RCATGY 1 cut(s) 740
BstSCI CCNGG 2 cut(s) 670, 2473
BstSFI CTRYAG 2 cut(s) 2210, 2258
BstSNI TACGTA 1 cut(s) 325
BstUI CGCG 1 cut(s) 181
BstV1I GCAGC 6 cut(s) 105, 259, 1666, 1751, 2552, 2739
BstV2I GAAGAC 1 cut(s) 2538
BstX2I RGATCY 2 cut(s) 1052, 1621
BstXI CCANNNNNNTGG 2 cut(s) 238, 763
BstYI RGATCY 2 cut(s) 1052, 1621
BstZ17I GTATAC 1 cut(s) 2299
BsuRI GGCC 5 cut(s) 69, 1213, 2403, 2473, 2612
BtgI CCRYGG 2 cut(s) 1208, 2625
BtsCI GGATG 7 cut(s) 349, 426, 772, 1111, 2515, 2637, 2685
BtsI GCAGTG 2 cut(s) 2299, 2810
BtsIMutI CAGTG 7 cut(s) 24, 291, 516, 2139, 2299, 2571, 2810
BveI ACCTGC 2 cut(s) 369, 2447
Cac8I GCNNGC 4 cut(s) 270, 682, 2419, 2799
CaiI CAGNNNCTG 1 cut(s) 2573
CciI TCATGA 2 cut(s) 1945, 2839
CfoI GCGC 2 cut(s) 1207, 1744
Cfr10I RCCGGY 3 cut(s) 1847, 2403, 2417
Cfr13I GGNCC 2 cut(s) 163, 2611
CseI GACGC 1 cut(s) 187
Csp6I GTAC 7 cut(s) 58, 85, 334, 871, 1757, 1825, 2057
CspAI ACCGGT 1 cut(s) 1847
CviQI GTAC 7 cut(s) 58, 85, 334, 871, 1757, 1825, 2057
DraI TTTAAA 2 cut(s) 1086, 2494
DraIII CACNNNGTG 1 cut(s) 1457
DrdI GACNNNNNNGTC 1 cut(s) 1231
DseDI GACNNNNNNGTC 1 cut(s) 1231
EaeI YGGCCR 1 cut(s) 2471
Eam1104I CTCTTC 2 cut(s) 1104, 2046
EarI CTCTTC 2 cut(s) 1104, 2046
Ecl136II GAGCTC 3 cut(s) 780, 2219, 2384
Eco105I TACGTA 1 cut(s) 325
Eco130I CCWWGG 4 cut(s) 756, 1208, 1434, 2625
Eco24I GRGCYC 3 cut(s) 782, 2221, 2386
Eco31I GGTCTC 1 cut(s) 2271
Eco32I GATATC 1 cut(s) 1725
Eco47I GGWCC 1 cut(s) 163
Eco47III AGCGCT 1 cut(s) 1743
Eco53kI GAGCTC 3 cut(s) 780, 2219, 2384
Eco57I CTGAAG 2 cut(s) 48, 2876
EcoICRI GAGCTC 3 cut(s) 780, 2219, 2384
EcoRI GAATTC 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 2473
EcoRV GATATC 1 cut(s) 1725
EcoT14I CCWWGG 4 cut(s) 756, 1208, 1434, 2625
EcoT38I GRGCYC 3 cut(s) 782, 2221, 2386
ErhI CCWWGG 4 cut(s) 756, 1208, 1434, 2625
FaqI GGGAC 1 cut(s) 2834
FblI GTMKAC 3 cut(s) 482, 2125, 2298
Fnu4HI GCNGC 8 cut(s) 119, 273, 1680, 1740, 1818, 2176, 2541, 2753
FokI GGATG 7 cut(s) 356, 433, 779, 1118, 2522, 2624, 2692
FriOI GRGCYC 3 cut(s) 782, 2221, 2386
Fsp4HI GCNGC 8 cut(s) 119, 273, 1680, 1740, 1818, 2176, 2541, 2753
FspBI CTAG 9 cut(s) 261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
GlaI GCGC 2 cut(s) 1206, 1743
GluI GCNGC 8 cut(s) 119, 273, 1680, 1740, 1818, 2176, 2541, 2753
GsuI CTGGAG 2 cut(s) 315, 1902
HaeII RGCGCY 1 cut(s) 1745
HaeIII GGCC 5 cut(s) 69, 1213, 2403, 2473, 2612
HapII CCGG 4 cut(s) 671, 1848, 2404, 2418
HgaI GACGC 1 cut(s) 187
HhaI GCGC 2 cut(s) 1207, 1744
Hin6I GCGC 2 cut(s) 1205, 1742
HinP1I GCGC 2 cut(s) 1205, 1742
HincII GTYRAC 1 cut(s) 483
HindII GTYRAC 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 611
HpaII CCGG 4 cut(s) 671, 1848, 2404, 2418
HphI GGTGA 7 cut(s) 403, 660, 803, 1297, 1469, 2086, 2720
Hpy166II GTNNAC 5 cut(s) 483, 607, 1060, 2126, 2299
Hpy8I GTNNAC 5 cut(s) 483, 607, 1060, 2126, 2299
Hpy99I CGWCG 2 cut(s) 181, 487
HpyCH4III ACNGT 8 cut(s) 511, 791, 1276, 1795, 2342, 2465, 2575, 2671
HpyCH4IV ACGT 5 cut(s) 324, 336, 537, 2348, 2355
HpyF10VI GCNNNNNNNGC 9 cut(s) 296, 384, 1287, 1362, 1383, 1486, 2318, 2423, 2752
HpySE526I ACGT 5 cut(s) 324, 336, 537, 2348, 2355
HspAI GCGC 2 cut(s) 1205, 1742
KpnI GGTACC 1 cut(s) 61
KroI GCCGGC 1 cut(s) 2417
KroNI GCCGGC 1 cut(s) 2419
Lsp1109I GCAGC 6 cut(s) 105, 259, 1666, 1751, 2552, 2739
LweI GCATC 7 cut(s) 757, 1247, 1773, 2255, 2404, 2646, 2686
MaeI CTAG 9 cut(s) 261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
MaeII ACGT 5 cut(s) 324, 336, 537, 2348, 2355
MaeIII GTNAC 4 cut(s) 320, 2459, 2665, 2908
MfeI CAATTG 1 cut(s) 600
MflI RGATCY 2 cut(s) 1052, 1621
MhlI GDGCHC 4 cut(s) 586, 782, 2221, 2386
MlsI TGGCCA 1 cut(s) 2473
MluNI TGGCCA 1 cut(s) 2473
MlyI GAGTC 4 cut(s) 30, 1226, 1442, 1936
MmeI TCCRAC 4 cut(s) 200, 1425, 1491, 2683
Mox20I TGGCCA 1 cut(s) 2473
MroNI GCCGGC 1 cut(s) 2417
MroXI GAANNNNTTC 1 cut(s) 77
MscI TGGCCA 1 cut(s) 2473
MseI TTAA 7 cut(s) 204, 534, 1085, 1361, 2181, 2240, 2493
MslI CAYNNNNRTG 1 cut(s) 2844
Msp20I TGGCCA 1 cut(s) 2473
MspA1I CMGCKG 2 cut(s) 2752, 2867
MspCI CTTAAG 1 cut(s) 1360
MspI CCGG 4 cut(s) 671, 1848, 2404, 2418
MspR9I CCNGG 2 cut(s) 672, 2475
MunI CAATTG 1 cut(s) 600
Mva1269I GAATGC 3 cut(s) 1270, 1882, 2090
MvaI CCWGG 1 cut(s) 2475
MvnI CGCG 1 cut(s) 181
MwoI GCNNNNNNNGC 9 cut(s) 296, 384, 1287, 1362, 1383, 1486, 2318, 2423, 2752
NaeI GCCGGC 1 cut(s) 2419
NciI CCSGG 1 cut(s) 672
NcoI CCATGG 2 cut(s) 1208, 2625
NgoMIV GCCGGC 1 cut(s) 2417
NlaIV GGNNCC 6 cut(s) 59, 174, 547, 906, 1036, 2086
NmuCI GTSAC 2 cut(s) 2665, 2908
NspI RCATGY 1 cut(s) 740
PagI TCATGA 2 cut(s) 1945, 2839
PciI ACATGT 1 cut(s) 736
PcsI WCGNNNNNNNCGW 2 cut(s) 221, 251
PctI GAATGC 3 cut(s) 1270, 1882, 2090
PdiI GCCGGC 1 cut(s) 2419
PdmI GAANNNNTTC 1 cut(s) 77
PfeI GAWTC 8 cut(s) 101, 437, 523, 665, 980, 1248, 1294, 2621
PflFI GACNNNGTC 2 cut(s) 2671, 2731
PflMI CCANNNNNTGG 1 cut(s) 1440
PinAI ACCGGT 1 cut(s) 1847
PkrI GCNGC 8 cut(s) 120, 274, 1681, 1741, 1819, 2177, 2542, 2754
Ple19I CGATCG 1 cut(s) 2657
PleI GAGTC 4 cut(s) 30, 1225, 1442, 1936
PpsI GAGTC 4 cut(s) 30, 1225, 1442, 1936
Ppu21I YACGTR 3 cut(s) 325, 337, 2356
PscI ACATGT 1 cut(s) 736
Psp124BI GAGCTC 3 cut(s) 782, 2221, 2386
Psp1406I AACGTT 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 2473
PspGI CCWGG 1 cut(s) 2473
PspN4I GGNNCC 6 cut(s) 59, 174, 547, 906, 1036, 2086
PspPI GGNCC 2 cut(s) 163, 2611
PsrI GAACNNNNNNTAC 2 cut(s) 2040, 2072
PstI CTGCAG 1 cut(s) 2214
PstNI CAGNNNCTG 1 cut(s) 2573
PsuI RGATCY 2 cut(s) 1052, 1621
PsyI GACNNNGTC 2 cut(s) 2671, 2731
PvuI CGATCG 1 cut(s) 2657
PvuII CAGCTG 1 cut(s) 2752
RsaI GTAC 7 cut(s) 59, 86, 335, 872, 1758, 1826, 2058
RsaNI GTAC 7 cut(s) 58, 85, 334, 871, 1757, 1825, 2057
RseI CAYNNNNRTG 1 cut(s) 2844
SacI GAGCTC 3 cut(s) 782, 2221, 2386
SalI GTCGAC 1 cut(s) 481
SaqAI TTAA 7 cut(s) 204, 534, 1085, 1361, 2181, 2240, 2493
SatI GCNGC 8 cut(s) 119, 273, 1680, 1740, 1818, 2176, 2541, 2753
Sau96I GGNCC 2 cut(s) 163, 2611
ScaI AGTACT 1 cut(s) 1758
SchI GAGTC 4 cut(s) 30, 1226, 1442, 1936
ScrFI CCNGG 2 cut(s) 672, 2475
SduI GDGCHC 4 cut(s) 586, 782, 2221, 2386
SfaNI GCATC 7 cut(s) 757, 1247, 1773, 2255, 2404, 2646, 2686
SfcI CTRYAG 2 cut(s) 2210, 2258
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 2844
SmlI CTYRAG 4 cut(s) 614, 1043, 1360, 1809
SmoI CTYRAG 4 cut(s) 614, 1043, 1360, 1809
SnaBI TACGTA 1 cut(s) 325
SpeI ACTAGT 1 cut(s) 260
SsiI CCGC 4 cut(s) 1817, 2088, 2175, 2867
SspMI CTAG 9 cut(s) 261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
SstI GAGCTC 3 cut(s) 782, 2221, 2386
StyD4I CCNGG 2 cut(s) 670, 2473
StyI CCWWGG 4 cut(s) 756, 1208, 1434, 2625
TaaI ACNGT 8 cut(s) 511, 791, 1276, 1795, 2342, 2465, 2575, 2671
TaiI ACGT 5 cut(s) 327, 339, 540, 2351, 2358
TatI WGTACW 4 cut(s) 84, 870, 1756, 2056
TauI GCSGC 2 cut(s) 1820, 2178
TfiI GAWTC 8 cut(s) 101, 437, 523, 665, 980, 1248, 1294, 2621
Tru1I TTAA 7 cut(s) 204, 534, 1085, 1361, 2181, 2240, 2493
Tru9I TTAA 7 cut(s) 204, 534, 1085, 1361, 2181, 2240, 2493
TscAI CASTG 7 cut(s) 31, 298, 516, 2146, 2299, 2578, 2817
TseFI GTSAC 2 cut(s) 2665, 2908
TseI GCWGC 6 cut(s) 118, 272, 1679, 1739, 2540, 2752
Tsp45I GTSAC 2 cut(s) 2665, 2908
TspGWI ACGGA 2 cut(s) 356, 2561
TspRI CASTG 7 cut(s) 31, 298, 516, 2146, 2299, 2578, 2817
Tth111I GACNNNGTC 2 cut(s) 2671, 2731
Van91I CCANNNNNTGG 1 cut(s) 1440
Vha464I CTTAAG 1 cut(s) 1360
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 7 cut(s) 226, 517, 553, 1012, 1542, 1635, 2479
XceI RCATGY 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 2 cut(s) 298, 1441
XmiI GTMKAC 3 cut(s) 482, 2125, 2298
XmnI GAANNNNTTC 1 cut(s) 77
XspI CTAG 9 cut(s) 261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
ZrmI AGTACT 1 cut(s) 1758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.