pycom05g24560

Protein prune homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006920
Physical Location & Seq
Forward (+)
26101948 .. 26106448
4501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1332 bp
ATGAGCCTGCCATTGAAGCAAATGATCGATAAGCATGGTAGAGTAAATGGTCTAGCAAGTGTCACAAGTGGGAAATCATTCAGGGTGAATAGAAGGAAAGAAAACCTGTCTACTGTTTCACTTCCTCAGTCTGCTGCATCGTTTTATAGTGGTGACTCACCGCAATTTGAAATTGTTGCATCGTGTCCAAGCATTTACAGGCTTAATTTGTTTCTCAAAGCCACAAAGGATGAAGTTAATGCCGGTGTCCCGGGAAAATTCTTACATGTTGTAATGGCTCAAGATATTGCTGATGTTGGATCCATTGTTTCAACCATCACTTATGCCTTCTACCTGAATGAAACACAAAAATGTGACCAGTTGTGTACAGTGCCTGTTATCAACATGAAGAGGTCAGACATAAATTCTCAAGCTGAACTCAAATGGTTACTTGATTTTTGTCAAATTGATCAGTCGTCCTTAATCTTTGTGGATGAGATTGATCTGTCATATTATGATCTATTTGGGAGTCTTAAAATAGTTCTATTAAATGGCCACAAGCTCCCAGCCAAGCAAGAGGGATTGAAAGAGGCAGTAGTTGAATTTTTCAATTGCAAACAGGGAGAATCTGTATATCCCTGGGTTAAGAGTGTTACTGTAGCAGAAGATAGCTCATGCTGCACAGTTGTTGCTGAGAAGTTTTCTCTGATTTTACCTGAGATTTTGGCTGGACAAGGATTCAGCAGATTTTTGTTAGCTGCCATCCTTTTAGACTCCACAAACTTGTGTGGTTCTCAGTGTTCAATCAAAGATAAGTACATGGCTACACTGTTAATTCATGGTGCTGGTCGCTTTGGGTGCAACGGCCTTTACCAGTTATTGAGATACAAAATGTATGATGTATCTGATCTCAAGGTTGGTGATATATTACGCAAGGATTTCAAAAAGTGGACAAGAGCAGGTAAACCGGAGGGTCCCAGTGCTAGAACATCACAAATTGGGATGAGTTCCATTGGAATGCCAATTGCACAGCTTCTTCAACATGAGAACACCTCAGCGCATGAAGTTAAAAATTTCCAACAGATGGAGAAACTCCGGCTTCTCATGGTTGTTTCTGGGTATTATGATTCGAAAAGGAACTTCAAGAGAGAGATCCTAGTGGCTGCTGAGTCAGTGGAGCTCATGAAAAATCTGCTGTTTTTCTTCGACTCAAGTGCTTCCCAACTGCCTCTCAAAGCGATCCATCTGTCAGGTCTTGGAGCAGAGATGCAAGCATTTGAGATTGACAAGGTCACATCGAGAAAGACCATCGAGCGCCTCTTAGAAGAATTTGGTGGGACTTCAAAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
DHHA2

Protein Analysis

444

Amino Acids

49.25

Weight (kDa)

8.39

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHA2 PF02833 290 - 428 3.1e-10 DHHA2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 929
AccB7I CCANNNNNTGG 1 cut(s) 1063
AccI GTMKAC 1 cut(s) 110
AciI CCGC 1 cut(s) 161
AclWI GGATC 4 cut(s) 294, 307, 1126, 1213
AcoI YGGCCR 1 cut(s) 532
AcsI RAATTY 5 cut(s) 257, 403, 581, 1051, 1307
AfaI GTAC 2 cut(s) 367, 797
AfiI CCNNNNNNNGG 1 cut(s) 1063
AflIII ACRYGT 1 cut(s) 265
AjnI CCWGG 1 cut(s) 617
AluBI AGCT 6 cut(s) 413, 541, 651, 737, 1012, 1159
AluI AGCT 6 cut(s) 413, 541, 651, 737, 1012, 1159
Alw21I GWGCWC 1 cut(s) 1161
AlwI GGATC 4 cut(s) 294, 307, 1126, 1213
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 250
AoxI GGCC 2 cut(s) 532, 844
ApeKI GCWGC 4 cut(s) 134, 657, 737, 1142
ApoI RAATTY 5 cut(s) 257, 403, 581, 1051, 1307
Asp700I GAANNNNTTC 1 cut(s) 77
AspLEI GCGC 2 cut(s) 1039, 1296
AspS9I GGNCC 1 cut(s) 953
AsuC2I CCSGG 2 cut(s) 251, 252
AsuHPI GGTGA 4 cut(s) 97, 150, 164, 911
AsuII TTCGAA 1 cut(s) 1109
AvaI CYCGRG 1 cut(s) 250
AvaII GGWCC 1 cut(s) 953
BalI TGGCCA 1 cut(s) 534
BamHI GGATCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 1161
Bbv12I GWGCWC 1 cut(s) 1161
BbvCI CCTCAGC 1 cut(s) 1033
BbvI GCAGC 4 cut(s) 121, 644, 724, 1129
BccI CCATC 5 cut(s) 323, 749, 1057, 1230, 1295
BceAI ACGGC 1 cut(s) 859
BcgI CGANNNNNNTGC 2 cut(s) 1175, 1209
BciT130I CCWGG 1 cut(s) 619
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 2 cut(s) 251, 252
BfaI CTAG 3 cut(s) 53, 963, 1136
BfmI CTRYAG 1 cut(s) 636
BfoI RGCGCY 1 cut(s) 1297
BfuAI ACCTGC 1 cut(s) 929
BisI GCNGC 4 cut(s) 135, 658, 738, 1143
BlsI GCNGC 4 cut(s) 136, 659, 739, 1144
Bme1390I CCNGG 3 cut(s) 251, 252, 619
Bme18I GGWCC 1 cut(s) 953
BmeT110I CYCGRG 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 953
BmiI GGNNCC 3 cut(s) 301, 954, 955
BmrFI CCNGG 3 cut(s) 251, 252, 619
BmrI ACTGGG 1 cut(s) 951
BmsI GCATC 3 cut(s) 146, 188, 1236
BmuI ACTGGG 1 cut(s) 951
BplI GAGNNNNNCTC 2 cut(s) 1016, 1048
Bpu10I CCTNAGC 1 cut(s) 1033
Bpu14I TTCGAA 1 cut(s) 1109
BpuEI CTTGAG 4 cut(s) 264, 393, 875, 1174
BpuMI CCSGG 2 cut(s) 251, 252
Bsa29I ATCGAT 1 cut(s) 27
BsaJI CCNNGG 3 cut(s) 250, 617, 618
BsaWI WCCGGW 1 cut(s) 946
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 1063
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 3 cut(s) 358, 853, 957
BseBI CCWGG 1 cut(s) 619
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 3 cut(s) 250, 617, 618
BseGI GGATG 4 cut(s) 235, 478, 741, 987
BseLI CCNNNNNNNGG 1 cut(s) 1063
BseMII CTCAG 6 cut(s) 140, 663, 687, 788, 1047, 1137
BseNI ACTGG 3 cut(s) 358, 853, 957
BseXI GCAGC 4 cut(s) 121, 644, 724, 1129
BseYI CCCAGC 1 cut(s) 544
BsgI GTGCAG 1 cut(s) 643
BshFI GGCC 2 cut(s) 534, 846
BshVI ATCGAT 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 1161
BsiHKCI CYCGRG 1 cut(s) 250
BsiSI CCGG 4 cut(s) 243, 251, 947, 1075
BslFI GGGAC 2 cut(s) 233, 939
BslI CCNNNNNNNGG 1 cut(s) 1063
BsmFI GGGAC 2 cut(s) 233, 939
BsmI GAATGC 1 cut(s) 1002
BsnI GGCC 2 cut(s) 534, 846
BsoBI CYCGRG 1 cut(s) 250
Bsp119I TTCGAA 1 cut(s) 1109
Bsp1286I GDGCHC 1 cut(s) 1161
Bsp1407I TGTACA 1 cut(s) 365
Bsp143I GATC 8 cut(s) 24, 299, 448, 481, 496, 886, 1131, 1218
BspACI CCGC 1 cut(s) 161
BspANI GGCC 2 cut(s) 534, 846
BspCNI CTCAG 6 cut(s) 139, 664, 688, 787, 1046, 1138
BspDI ATCGAT 1 cut(s) 27
BspHI TCATGA 1 cut(s) 1161
BspLI GGNNCC 3 cut(s) 301, 954, 955
BspMI ACCTGC 1 cut(s) 929
BspPI GGATC 4 cut(s) 294, 307, 1126, 1213
BspT104I TTCGAA 1 cut(s) 1109
BsrFI RCCGGY 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 365
BsrI ACTGG 3 cut(s) 358, 853, 957
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 3 cut(s) 250, 617, 618
BssMI GATC 8 cut(s) 24, 299, 448, 481, 496, 886, 1131, 1218
Bst2UI CCWGG 1 cut(s) 619
Bst4CI ACNGT 5 cut(s) 115, 370, 637, 664, 810
Bst6I CTCTTC 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 365
BstBI TTCGAA 1 cut(s) 1109
BstC8I GCNNGC 2 cut(s) 8, 1251
BstDEI CTNAG 7 cut(s) 126, 672, 696, 774, 1033, 1146, 1300
BstF5I GGATG 4 cut(s) 235, 478, 741, 987
BstH2I RGCGCY 1 cut(s) 1297
BstHHI GCGC 2 cut(s) 1039, 1296
BstKTI GATC 8 cut(s) 27, 302, 451, 484, 499, 889, 1134, 1221
BstMBI GATC 8 cut(s) 24, 299, 448, 481, 496, 886, 1131, 1218
BstMWI GCNNNNNNNGC 3 cut(s) 16, 657, 837
BstNI CCWGG 1 cut(s) 619
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 3 cut(s) 249, 250, 617
BstSFI CTRYAG 1 cut(s) 636
BstV1I GCAGC 4 cut(s) 121, 644, 724, 1129
BstX2I RGATCY 2 cut(s) 299, 1131
BstYI RGATCY 2 cut(s) 299, 1131
Bsu15I ATCGAT 1 cut(s) 27
BsuRI GGCC 2 cut(s) 534, 846
BsuTUI ATCGAT 1 cut(s) 27
BtsCI GGATG 4 cut(s) 235, 478, 741, 987
BtsIMutI CAGTG 5 cut(s) 375, 782, 806, 964, 1158
BveI ACCTGC 1 cut(s) 929
Cac8I GCNNGC 2 cut(s) 8, 1251
CaiI CAGNNNCTG 1 cut(s) 374
CciI TCATGA 1 cut(s) 1161
CfoI GCGC 2 cut(s) 1039, 1296
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 953
Cfr9I CCCGGG 1 cut(s) 250
ClaI ATCGAT 1 cut(s) 27
Csp6I GTAC 2 cut(s) 366, 796
CviQI GTAC 2 cut(s) 366, 796
DdeI CTNAG 7 cut(s) 126, 672, 696, 774, 1033, 1146, 1300
DpnI GATC 8 cut(s) 26, 301, 450, 483, 498, 888, 1133, 1220
DpnII GATC 8 cut(s) 24, 299, 448, 481, 496, 886, 1131, 1218
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 383
Ecl136II GAGCTC 1 cut(s) 1159
Eco24I GRGCYC 1 cut(s) 1161
Eco47I GGWCC 1 cut(s) 953
Eco53kI GAGCTC 1 cut(s) 1159
Eco88I CYCGRG 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 1159
EcoO109I RGGNCCY 1 cut(s) 953
EcoRII CCWGG 1 cut(s) 617
EcoT38I GRGCYC 1 cut(s) 1161
FaqI GGGAC 2 cut(s) 233, 939
FbaI TGATCA 1 cut(s) 448
FblI GTMKAC 1 cut(s) 110
Fnu4HI GCNGC 4 cut(s) 135, 658, 738, 1143
FokI GGATG 4 cut(s) 242, 485, 728, 994
FriOI GRGCYC 1 cut(s) 1161
Fsp4HI GCNGC 4 cut(s) 135, 658, 738, 1143
FspBI CTAG 3 cut(s) 53, 963, 1136
GlaI GCGC 2 cut(s) 1038, 1295
GluI GCNGC 4 cut(s) 135, 658, 738, 1143
GsaI CCCAGC 1 cut(s) 548
HaeII RGCGCY 1 cut(s) 1297
HaeIII GGCC 2 cut(s) 534, 846
HapII CCGG 4 cut(s) 243, 251, 947, 1075
HhaI GCGC 2 cut(s) 1039, 1296
Hin6I GCGC 2 cut(s) 1037, 1294
HinP1I GCGC 2 cut(s) 1037, 1294
HinfI GANTC 8 cut(s) 155, 508, 605, 717, 752, 1106, 1148, 1187
HpaII CCGG 4 cut(s) 243, 251, 947, 1075
HphI GGTGA 4 cut(s) 97, 150, 164, 911
Hpy166II GTNNAC 4 cut(s) 111, 366, 930, 944
Hpy188I TCNGA 3 cut(s) 397, 687, 886
Hpy188III TCNNGA 4 cut(s) 281, 1123, 1162, 1278
Hpy8I GTNNAC 4 cut(s) 111, 366, 930, 944
HpyAV CCTTC 2 cut(s) 87, 337
HpyCH4III ACNGT 5 cut(s) 115, 370, 637, 664, 810
HpyCH4V TGCA 7 cut(s) 137, 179, 594, 660, 840, 1007, 1249
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 657, 837
HpyF3I CTNAG 7 cut(s) 126, 672, 696, 774, 1033, 1146, 1300
HspAI GCGC 2 cut(s) 1037, 1294
KflI GGGWCCC 1 cut(s) 953
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 8 cut(s) 24, 299, 448, 481, 496, 886, 1131, 1218
LmnI GCTCC 3 cut(s) 546, 1156, 1238
Lsp1109I GCAGC 4 cut(s) 121, 644, 724, 1129
LweI GCATC 3 cut(s) 146, 188, 1236
MaeI CTAG 3 cut(s) 53, 963, 1136
MaeIII GTNAC 6 cut(s) 61, 152, 353, 426, 631, 1270
MalI GATC 8 cut(s) 26, 301, 450, 483, 498, 888, 1133, 1220
MboI GATC 8 cut(s) 24, 299, 448, 481, 496, 886, 1131, 1218
MboII GAAGA 5 cut(s) 400, 656, 1007, 1174, 1316
MfeI CAATTG 2 cut(s) 589, 1002
MflI RGATCY 2 cut(s) 299, 1131
MhlI GDGCHC 1 cut(s) 1161
MlsI TGGCCA 1 cut(s) 534
MluNI TGGCCA 1 cut(s) 534
MlyI GAGTC 5 cut(s) 149, 517, 746, 1157, 1181
MmeI TCCRAC 2 cut(s) 277, 1081
MnlI CCTC 8 cut(s) 135, 384, 550, 562, 943, 1042, 1218, 1307
Mox20I TGGCCA 1 cut(s) 534
MroXI GAANNNNTTC 1 cut(s) 77
MscI TGGCCA 1 cut(s) 534
MseI TTAA 8 cut(s) 204, 237, 461, 513, 527, 624, 812, 1047
MslI CAYNNNNRTG 2 cut(s) 349, 995
Msp20I TGGCCA 1 cut(s) 534
MspI CCGG 4 cut(s) 243, 251, 947, 1075
MspR9I CCNGG 3 cut(s) 251, 252, 619
MunI CAATTG 2 cut(s) 589, 1002
Mva1269I GAATGC 1 cut(s) 1002
MvaI CCWGG 1 cut(s) 619
MwoI GCNNNNNNNGC 3 cut(s) 16, 657, 837
NciI CCSGG 2 cut(s) 251, 252
NdeII GATC 8 cut(s) 24, 299, 448, 481, 496, 886, 1131, 1218
NlaIV GGNNCC 3 cut(s) 301, 954, 955
NmuCI GTSAC 4 cut(s) 61, 152, 353, 1270
NspI RCATGY 1 cut(s) 269
NspV TTCGAA 1 cut(s) 1109
PagI TCATGA 1 cut(s) 1161
PasI CCCWGGG 1 cut(s) 618
PciI ACATGT 1 cut(s) 265
PctI GAATGC 1 cut(s) 1002
PdmI GAANNNNTTC 1 cut(s) 77
PfeI GAWTC 3 cut(s) 605, 717, 1106
PflFI GACNNNGTC 1 cut(s) 1268
PflMI CCANNNNNTGG 1 cut(s) 1063
PkrI GCNGC 4 cut(s) 136, 659, 739, 1144
PleI GAGTC 5 cut(s) 149, 516, 746, 1156, 1181
PpsI GAGTC 5 cut(s) 149, 516, 746, 1156, 1181
PpuMI RGGWCCY 1 cut(s) 953
PscI ACATGT 1 cut(s) 265
Psp124BI GAGCTC 1 cut(s) 1161
Psp5II RGGWCCY 1 cut(s) 953
Psp6I CCWGG 1 cut(s) 617
PspFI CCCAGC 1 cut(s) 544
PspGI CCWGG 1 cut(s) 617
PspN4I GGNNCC 3 cut(s) 301, 954, 955
PspPI GGNCC 1 cut(s) 953
PspPPI RGGWCCY 1 cut(s) 953
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 2 cut(s) 299, 1131
PsyI GACNNNGTC 1 cut(s) 1268
RsaI GTAC 2 cut(s) 367, 797
RsaNI GTAC 2 cut(s) 366, 796
RseI CAYNNNNRTG 2 cut(s) 349, 995
SacI GAGCTC 1 cut(s) 1161
SaqAI TTAA 8 cut(s) 204, 237, 461, 513, 527, 624, 812, 1047
SatI GCNGC 4 cut(s) 135, 658, 738, 1143
Sau3AI GATC 8 cut(s) 24, 299, 448, 481, 496, 886, 1131, 1218
Sau96I GGNCC 1 cut(s) 953
SchI GAGTC 5 cut(s) 149, 517, 746, 1157, 1181
ScrFI CCNGG 3 cut(s) 251, 252, 619
SduI GDGCHC 1 cut(s) 1161
SfaNI GCATC 3 cut(s) 146, 188, 1236
SfcI CTRYAG 1 cut(s) 636
SfuI TTCGAA 1 cut(s) 1109
SinI GGWCC 1 cut(s) 953
SmaI CCCGGG 1 cut(s) 252
SmiMI CAYNNNNRTG 2 cut(s) 349, 995
SmlI CTYRAG 4 cut(s) 279, 408, 890, 1189
SmoI CTYRAG 4 cut(s) 279, 408, 890, 1189
SsiI CCGC 1 cut(s) 161
SspMI CTAG 3 cut(s) 53, 963, 1136
SstI GAGCTC 1 cut(s) 1161
StyD4I CCNGG 3 cut(s) 249, 250, 617
TaaI ACNGT 5 cut(s) 115, 370, 637, 664, 810
TaqI TCGA 5 cut(s) 27, 1109, 1185, 1277, 1290
TatI WGTACW 2 cut(s) 365, 795
TfiI GAWTC 3 cut(s) 605, 717, 1106
Tru1I TTAA 8 cut(s) 204, 237, 461, 513, 527, 624, 812, 1047
Tru9I TTAA 8 cut(s) 204, 237, 461, 513, 527, 624, 812, 1047
TscAI CASTG 5 cut(s) 375, 782, 813, 964, 1158
TseFI GTSAC 4 cut(s) 61, 152, 353, 1270
TseI GCWGC 4 cut(s) 134, 657, 737, 1142
Tsp45I GTSAC 4 cut(s) 61, 152, 353, 1270
TspDTI ATGAA 6 cut(s) 246, 354, 401, 806, 1056, 1178
TspMI CCCGGG 1 cut(s) 250
TspRI CASTG 5 cut(s) 375, 782, 813, 964, 1158
Tth111I GACNNNGTC 1 cut(s) 1268
Van91I CCANNNNNTGG 1 cut(s) 1063
VpaK11BI GGWCC 1 cut(s) 953
XapI RAATTY 5 cut(s) 257, 403, 581, 1051, 1307
XceI RCATGY 1 cut(s) 269
XmaI CCCGGG 1 cut(s) 250
XmiI GTMKAC 1 cut(s) 110
XmnI GAANNNNTTC 1 cut(s) 77
XspI CTAG 3 cut(s) 53, 963, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.