pycom05g25710

Photosystem I reaction center subunit psaK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015469
Physical Location & Seq
Reverse (-)
27060828 .. 27061637
810 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 393 bp
ATGGCAGCTACCACTGTGACCACTTCTCTCCCCCAGTTCAACGGGCTCAGACCCAAAATCTCCTTCTCTAATGTGCAAAGCATGGCGGTTGCTCAACCAATGAGGCGCAAAGGCCAGGGCGCTTTGGGAGCTCGCTGCGATTTCATTGGTTCATCCACCAATTTGATAATGGTGACTACAACAAGCCTAATGTTGTTTGCTGGAAGATTCGGGTTGGCCCCTTCGGCAAACAGGAAGGCAACGGCAGGATTGAAGCTTGAAACAAGGGACTCTGGGCTACAGACTGGCGACCCGGCTGGGTTTACACTTGCTGATACCTTGGCCTGTGGGACAGTTGGTCACATTATTGGGGTTGGTGTTGTTCTTGGCCTTAAGAATGTTGGTGCCCTGTAA

Protein Analysis

131

Amino Acids

13.31

Weight (kDa)

10.52

Isoelectric Point (pI)

28.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PSAK PF01241 52 - 128 4.1e-18 Photosystem I psaG / psaK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30380
fragaria_vesca FvH4_3g09680
malus_domestica MD00G1109700.v1.1 MD05G1276100.v1.1
prunus_persica Prupe.4G088200_v2.0.a1
pyrus_communis pycom05g25710 pycom10g21120
rosa_chinensis RchiOBHm_Chr5g0015361
rosa_laevigata RLG00000032195
rosa_multiflora Rmu_sc0014430.1_g000001
rosa_rugosa Rorug05G0026800
rosa_samantha Rh5AG120400 Rh5BG119400 Rh5CG129800 Rh5DG118400
rosa_wichuraiana Rw5G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 383
AciI CCGC 1 cut(s) 86
AflII CTTAAG 1 cut(s) 371
AgsI TTSAA 3 cut(s) 40, 253, 260
AhdI GACNNNNNGTC 1 cut(s) 336
AjnI CCWGG 1 cut(s) 114
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
AluBI AGCT 3 cut(s) 8, 131, 256
AluI AGCT 3 cut(s) 8, 131, 256
Alw21I GWGCWC 1 cut(s) 133
AoxI GGCC 4 cut(s) 112, 216, 321, 367
ApeKI GCWGC 2 cut(s) 5, 135
AspLEI GCGC 2 cut(s) 108, 122
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 2 cut(s) 48, 133
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 2 cut(s) 17, 122
BceAI ACGGC 1 cut(s) 258
BciT130I CCWGG 1 cut(s) 116
BcnI CCSGG 1 cut(s) 293
BfmI CTRYAG 1 cut(s) 278
BfoI RGCGCY 1 cut(s) 123
BfrI CTTAAG 1 cut(s) 371
BglI GCCNNNNNGGC 1 cut(s) 224
BisI GCNGC 2 cut(s) 6, 136
BlsI GCNGC 2 cut(s) 7, 137
Bme1390I CCNGG 2 cut(s) 116, 293
BmeRI GACNNNNNGTC 1 cut(s) 336
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 2 cut(s) 219, 385
BmrFI CCNGG 2 cut(s) 116, 293
BmrI ACTGGG 1 cut(s) 28
BmuI ACTGGG 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 115, 318
Bse1I ACTGG 2 cut(s) 34, 289
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 2 cut(s) 115, 318
BseGI GGATG 1 cut(s) 152
BseMII CTCAG 1 cut(s) 61
BseNI ACTGG 2 cut(s) 34, 289
BseSI GKGCMC 1 cut(s) 388
BseXI GCAGC 2 cut(s) 17, 122
BseYI CCCAGC 1 cut(s) 296
BshFI GGCC 4 cut(s) 114, 218, 323, 369
BshNI GGYRCC 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 133
BsiSI CCGG 1 cut(s) 293
BslFI GGGAC 2 cut(s) 281, 343
BsmFI GGGAC 2 cut(s) 281, 343
BsnI GGCC 4 cut(s) 114, 218, 323, 369
Bsp1286I GDGCHC 3 cut(s) 48, 133, 388
BspACI CCGC 1 cut(s) 86
BspANI GGCC 4 cut(s) 114, 218, 323, 369
BspCNI CTCAG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 219, 385
BspT107I GGYRCC 1 cut(s) 383
BspTI CTTAAG 1 cut(s) 371
BsrI ACTGG 2 cut(s) 34, 289
BssECI CCNNGG 2 cut(s) 115, 318
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 16, 334
BstAFI CTTAAG 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 133
BstDEI CTNAG 1 cut(s) 47
BstF5I GGATG 1 cut(s) 152
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 2 cut(s) 108, 122
BstMWI GCNNNNNNNGC 2 cut(s) 128, 224
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 2 cut(s) 114, 291
BstSFI CTRYAG 1 cut(s) 278
BstSLI GKGCMC 1 cut(s) 388
BstV1I GCAGC 2 cut(s) 17, 122
BsuRI GGCC 4 cut(s) 114, 218, 323, 369
BtsCI GGATG 1 cut(s) 152
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 1 cut(s) 133
CfoI GCGC 2 cut(s) 108, 122
Cfr13I GGNCC 1 cut(s) 217
CviAII CATG 1 cut(s) 82
DdeI CTNAG 1 cut(s) 47
DriI GACNNNNNGTC 1 cut(s) 336
Eam1105I GACNNNNNGTC 1 cut(s) 336
Ecl136II GAGCTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 318
Eco24I GRGCYC 2 cut(s) 48, 133
Eco53kI GAGCTC 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 318
EcoT38I GRGCYC 2 cut(s) 48, 133
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 1 cut(s) 85
FaiI YATR 1 cut(s) 83
FaqI GGGAC 2 cut(s) 281, 343
FatI CATG 1 cut(s) 81
Fnu4HI GCNGC 2 cut(s) 6, 136
FokI GGATG 1 cut(s) 139
FriOI GRGCYC 2 cut(s) 48, 133
Fsp4HI GCNGC 2 cut(s) 6, 136
GlaI GCGC 2 cut(s) 107, 121
GluI GCNGC 2 cut(s) 6, 136
GsaI CCCAGC 1 cut(s) 300
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 4 cut(s) 114, 218, 323, 369
HapII CCGG 1 cut(s) 293
HhaI GCGC 2 cut(s) 108, 122
Hin1II CATG 1 cut(s) 85
Hin6I GCGC 2 cut(s) 106, 120
HinP1I GCGC 2 cut(s) 106, 120
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 2 cut(s) 207, 269
HpaII CCGG 1 cut(s) 293
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 1 cut(s) 50
Hpy8I GTNNAC 1 cut(s) 303
HpyAV CCTTC 3 cut(s) 73, 229, 231
HpyCH4III ACNGT 2 cut(s) 16, 334
HpyCH4V TGCA 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 224
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 1 cut(s) 85
HspAI GCGC 2 cut(s) 106, 120
LmnI GCTCC 1 cut(s) 128
Lsp1109I GCAGC 2 cut(s) 17, 122
MaeIII GTNAC 3 cut(s) 16, 172, 338
MboII GAAGA 1 cut(s) 216
MhlI GDGCHC 3 cut(s) 48, 133, 388
MluCI AATT 1 cut(s) 160
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 1 cut(s) 96
MseI TTAA 1 cut(s) 372
MspCI CTTAAG 1 cut(s) 371
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 2 cut(s) 116, 293
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 128, 224
NciI CCSGG 1 cut(s) 293
NlaIII CATG 1 cut(s) 85
NlaIV GGNNCC 2 cut(s) 219, 385
NmuCI GTSAC 3 cut(s) 16, 172, 338
PfeI GAWTC 1 cut(s) 207
PkrI GCNGC 2 cut(s) 7, 137
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 133
Psp6I CCWGG 1 cut(s) 114
PspFI CCCAGC 1 cut(s) 296
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 2 cut(s) 219, 385
PspPI GGNCC 1 cut(s) 217
SacI GAGCTC 1 cut(s) 133
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 2 cut(s) 6, 136
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 2 cut(s) 116, 293
SduI GDGCHC 3 cut(s) 48, 133, 388
SetI ASST 4 cut(s) 10, 133, 258, 320
SfcI CTRYAG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 1 cut(s) 160
SsiI CCGC 1 cut(s) 86
SstI GAGCTC 1 cut(s) 133
StyD4I CCNGG 2 cut(s) 114, 291
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 2 cut(s) 16, 334
TasI AATT 1 cut(s) 160
TfiI GAWTC 1 cut(s) 207
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 3 cut(s) 16, 172, 338
TseI GCWGC 2 cut(s) 5, 135
Tsp45I GTSAC 3 cut(s) 16, 172, 338
TspDTI ATGAA 2 cut(s) 133, 141
TspRI CASTG 1 cut(s) 19
Vha464I CTTAAG 1 cut(s) 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.