pycom05g31510

Starch synthase 3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003232
Physical Location & Seq
Forward (+)
31236752 .. 31243597
6846 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3312 bp
ATGGAGGTTTCTATACATGCACAGAGGCCGCTCAGATGCAGAGGACTTTTCCAACCGGGAAGAAGCATCTTAAAGCTCCAACCTTTTACTGGGTTCTCTCCCCATGGAAGGTATTCTTCATGGTTCAAAGGAAACCTGACAAGTGGGGTTTCATACCAAATTACTGCCAGTTCGGCCGATTTTTCAAGGAGGAGACAAAGAAAAGTGTCTCCTGGTTCAAAGGGATTAGTGCCAGAAACCCCAGCAGGAACTAGCGTCCAGAAGACGAATCAAAGGAATATTGGGGACGAAAAGGGTATTACATCTTCGACATCCAGTGAGCTTGCTGGGAAAAATAAGAAAACACATCAGTTGAGGGTTGACACCAGAAGAGAATGGGCACTTGAACCTTCAGAAGAGAGCAATGTTGACGAAAAGAGAATTGATGAAACTAGTAGTAAAGTCGAGGAATCATCTTCAGTTGGCAAGCAGACAGCAATTGATAGGGGTATTCGAGGGATAGAAAATGGGAGTGTAGGTAAGGTCCTTGATGACATCTCAGATACTGATAAAAAGTTAACAAATGAAGACCCCTTAAAGTTAAAGTTGGAGAGGGAAGAAAAATTGCGTAAAGAGGAAATTGCGAGGCTTGCTGAGGAAAACTTCTCAAGAGGGAACAAAATATTTTTCTATCCTCAGGCCGTGAAACCTGATCAAGACATAGAAGTGTTCCTCAATAGAAGTATATCAACCCTAAGCAATGAGCCGGATGTTCAAATAATGGGAGCCTTTAACGACTGGCGTTGGAAATCTTTTACCTTCAGGTTGAACAAAACACAACTCAAGGGGGATTGGTGGTCTTGCCAGTTTCATGTTCCTAAAGAGTCATATAAAATAGACTTTGTGTTCTTTAATGGGCAAAATGTTTATGACAATAATGATGCAAAAGATTTCTGCATAACTGTGGAAGGTGGAATGGACCTCTTTGCATTTGAAGATTTCTTGCTTGAGGAGAAGCGTAAAGAACAAGAGAAACTTGCGAAGGAGCAAGCTGAGAGGGAAAGGCAAGCAGAAGAGCAGAGGCGAATTGAAGCGGAGAAGGCAGCAAGTGAAGCTGACAGAGCACAGGCAAAGGCAGAGATCGTAAAGAGACGACAAATGGTTCAAGAGTTGATAAAAAAGGCTGTAAGGTCTGTAGAAGACGTTTGGCACATAGAGCCTAAAGAGTTTAAAGGCGAGGACTTGGTGAAGTTATACTATAACCGAAGCTTAGGTCCTCTTGCTAATGTTAAGGAACTATGGATTCATGGGGGGCATAATGGTTGGAAGGATGGATTGTCAATTGTTGAAAGGCTCGTTAGCTCTGAGAGAAAGGATGGTGACTGGTGGTATGCCAATGTTGTTGTACCTGAACGAGCCGTTGTCTTGGATTGGGTTTTTGCTGATGGACCACCCCAAAATGCCGTTTTATATGATAACAACCACCGTCATGATTTCCATGCTATAGTTCCAAAATCCATTCCTGACGAACTATATTGGGTTGAGGAAGAACACCAGATATATAAGCAGCTCCAGGAGGAGAGGAGATTAAGGGAGGAGGCAATACGTGCCAAGGCCGAAAGAACAGCTCGTATGAAAGCAGAAATGAAGGAAAGAACTTTGAAAAGGTTTTTGCTGTCTCAGAAGCATATAGTATACACTGAGCCTCTTGATGTGCAAGCTGGTAACAAGGTGACAGTTTTCTACAATCCTGCCAACACAGTTCTGAATGGAAAGCCTGAGGTATGGTTCAGAGGATCATTTAACCGTTGGAACCATCGTAAGGGTCCATTGCAACCACAGAAGATGTTACCTTCGGAGAATGGTTCTCATGTCAAAACCACTGTTTCAGTTCCGTTGGATGCATACATGATGGACTTTGTTTTCTCTGAGACGGAAGATGGTGGACTTTTTGACAACAAAAATGGCATGGATTACCACATTCCAGTGTTTGGAGGAGTGGTTAAGGAGTCACCAATGAACATTGTTGGAGGTCTTGGTGATGTGGTTACGAGTCTCTCACGAGCTGTGCAAGACTTAAATCACCACGTCGACATCATTCTTCCAAAGTATGACTGTTTGAACCTTAGCAATGTGAAGGAGTTTCAGTATAACAGAGGCTTCTTTTGGGGAGGAACTGAAATAAAAGTTTGGTTTGGGAAAGTGGAAGGTGTTTCGGTCTACTTTTTGGAGCCTCAAAACAGATTCTTTCATGCAGGTTGCATATATGGTTGTAAAAATGATGCAGAGAGATTTGGTTTCTTTTGCCATGCTGCGCTAGAATTTCTACTCCAAAGTGGATTTCATCCTGATATCATTCATTGCCATGATTGGTCTAGTGCACCTGTTGCATGGTTATTTAAAGATCATTACATGCATTATGGTCTTAGTAAAGCTCGGATTGTTTTCACAATTCATAACCTTGAGTTTGGAGCGCCCTTCATTGGTAAAGCTGTGGCATATTCAGACAAGTCCACGACTGTATCCAACACTTATGCTAAGGAGGTATCTGGAAATCCTGCAGTTGCTCCGCATCTTTACAAGTTTCATGGTATAATAAATGGAATTGACCAAGATATATGGGATCCATACAATGATAAGTTCATTCCTCTATCATATACTTCTGAAAATGTTGTTGAAGGCAAACAAGCTGCCAAGGAAGCCTTGCAACAAAGGCTTGGTCTGAAAACAGCTGATCTCCCTGTAGTAGGTATTATCACTCGCTTAACTCACCAGAAAGGAATCCATCTCATCAAGCATGCCATTTGGCGTACCTTGGAACGCAATGGGCAGGTCGTATTGCTTGGGTCAGCTCCAGATCCTCGTATCCAAAATGATTTTGTAAATTTGGCAAATCAGTTACATTCTTCTCATGGTGATCGTGCTCGCCTCTGTTTAACTTATGATGAGCCACTGTCGCATTTGATATACGCTGGTGCTGATTTCATCTTAGTTCCTTCAATTTTTGAGCCATGCGGTCTCACCCAACTCATAGCAATGAGATATGGTTCAATACCTGTTGTTCGAAAAACTGGAGGACTTTATGATACGGTATTTGATGTTGACCATGATAAAGAGAGAGCAGACGTCCAGGGTGTCGAGCCAAATGGTTTCAGCTTTGATGGAGCTGATGCTGCTGGTGTCGATTATGCTCTTAATAGGGCAATCTCCGCTTGGTATGATGGTCGGGATTGGTTTAACTCGCTGTGCAAGACGGTGATGCAGCAAGATTGGTCTTGGAACAAGCCAGCTCTCGACTATATGGAGCTTTACCACGCAGCACGGAAGGTGTGA

Protein Analysis

1104

Amino Acids

125.86

Weight (kDa)

6.55

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM53 PF16760 235 - 294 8.5e-10 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 409 - 471 1.6e-14 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 571 - 633 3e-12 Starch/carbohydrate-binding module (family 53)
Glyco_transf_5 PF08323 665 - 843 1.9e-42 Starch synthase catalytic domain
Glyco_transf_4 PF13439 669 - 844 8.4e-09 Glycosyltransferase Family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 3108
Acc36I ACCTGC 2 cut(s) 2225, 2800
AccB7I CCANNNNNTGG 1 cut(s) 1970
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 3 cut(s) 1674, 2070, 2199
AciI CCGC 5 cut(s) 29, 1073, 2549, 2994, 3189
AclWI GGATC 4 cut(s) 1783, 2597, 2610, 2831
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 2 cut(s) 2300, 2863
AcuI CTGAAG 3 cut(s) 375, 441, 784
AcyI GRCGYC 1 cut(s) 3105
AfaI GTAC 2 cut(s) 1386, 2791
AfiI CCNNNNNNNGG 6 cut(s) 89, 108, 1801, 1970, 2462, 2726
AhlI ACTAGT 1 cut(s) 431
AjiI CACGTC 1 cut(s) 2068
AjnI CCWGG 3 cut(s) 211, 1551, 3108
AjuI GAANNNNNNNTTGG 2 cut(s) 2304, 2336
Alw21I GWGCWC 3 cut(s) 1105, 2362, 2905
Alw26I GTCTC 7 cut(s) 187, 213, 1123, 1662, 1904, 2039, 3002
Alw44I GTGCAC 1 cut(s) 2358
AlwI GGATC 4 cut(s) 1783, 2597, 2610, 2831
AlwNI CAGNNNCTG 1 cut(s) 545
AoxI GGCC 4 cut(s) 26, 174, 678, 1593
ApaLI GTGCAC 1 cut(s) 2358
ApeKI GCWGC 7 cut(s) 1082, 1546, 2291, 2669, 3152, 3241, 3296
ApoI RAATTY 2 cut(s) 2300, 2863
ArsI GACNNNNNNTTYG 4 cut(s) 1237, 1269, 2683, 2715
Asp700I GAANNNNTTC 1 cut(s) 112
AspLEI GCGC 2 cut(s) 2296, 2455
AspS9I GGNCC 5 cut(s) 523, 958, 1253, 1427, 1805
AsuC2I CCSGG 1 cut(s) 57
AsuII TTCGAA 1 cut(s) 3043
AvaII GGWCC 5 cut(s) 523, 958, 1253, 1427, 1805
AxyI CCTNAGG 2 cut(s) 675, 1758
BaeGI GKGCMC 2 cut(s) 382, 2362
BaeI ACNNNNGTAYC 2 cut(s) 2484, 2517
BamHI GGATCC 1 cut(s) 2602
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
BauI CACGAG 1 cut(s) 2040
BbsI GAAGAC 3 cut(s) 269, 573, 1185
Bbv12I GWGCWC 3 cut(s) 1105, 2362, 2905
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 7 cut(s) 1094, 1558, 2278, 2656, 3139, 3253, 3308
BccI CCATC 9 cut(s) 1304, 1349, 1418, 1803, 1885, 1913, 2772, 3134, 3194
BceAI ACGGC 3 cut(s) 665, 1382, 1427
BciT130I CCWGG 3 cut(s) 213, 1553, 3110
BciVI GTATCC 2 cut(s) 2512, 2855
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 7 cut(s) 187, 213, 1123, 1662, 1904, 2039, 3002
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 4 cut(s) 252, 432, 2297, 2355
BfmI CTRYAG 4 cut(s) 1173, 1482, 2538, 2721
BfoI RGCGCY 1 cut(s) 2456
BfuAI ACCTGC 2 cut(s) 2225, 2800
BfuI GTATCC 2 cut(s) 2512, 2855
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 8 cut(s) 29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
BlsI GCNGC 8 cut(s) 30, 1084, 1548, 2293, 2671, 3154, 3243, 3298
Bme1390I CCNGG 4 cut(s) 57, 213, 1553, 3110
Bme18I GGWCC 5 cut(s) 523, 958, 1253, 1427, 1805
BmgBI CACGTC 1 cut(s) 2068
BmgT120I GGNCC 5 cut(s) 523, 958, 1253, 1427, 1805
BmiI GGNNCC 5 cut(s) 766, 1793, 1806, 2211, 2604
BmrFI CCNGG 4 cut(s) 57, 213, 1553, 3110
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 8 cut(s) 26, 75, 910, 1870, 2251, 2560, 3139, 3228
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 3 cut(s) 269, 573, 1185
BpmI CTGGAG 3 cut(s) 1535, 2817, 3072
Bpu10I CCTNAGC 5 cut(s) 633, 734, 1249, 2105, 2517
Bpu14I TTCGAA 1 cut(s) 3043
BpuEI CTTGAG 4 cut(s) 631, 806, 1007, 2462
BpuMI CCSGG 1 cut(s) 57
BsaAI YACGTR 1 cut(s) 1586
BsaHI GRCGYC 1 cut(s) 3105
BsaI GGTCTC 1 cut(s) 3002
BsaJI CCNNGG 5 cut(s) 103, 1590, 2673, 2793, 3109
BsaXI ACNNNNNCTCC 6 cut(s) 502, 532, 1965, 1995, 2111, 2141
Bsc4I CCNNNNNNNGG 6 cut(s) 89, 108, 1801, 1970, 2462, 2726
Bse1I ACTGG 8 cut(s) 94, 168, 315, 782, 844, 1367, 1964, 3055
Bse21I CCTNAGG 2 cut(s) 675, 1758
Bse3DI GCAATG 7 cut(s) 409, 745, 1808, 2116, 2338, 2809, 3021
BseBI CCWGG 3 cut(s) 213, 1553, 3110
BseDI CCNNGG 5 cut(s) 103, 1590, 2673, 2793, 3109
BseGI GGATG 6 cut(s) 311, 754, 1315, 1360, 1885, 2323
BseLI CCNNNNNNNGG 6 cut(s) 89, 108, 1801, 1970, 2462, 2726
BseMI GCAATG 7 cut(s) 409, 745, 1808, 2116, 2338, 2809, 3021
BseNI ACTGG 8 cut(s) 94, 168, 315, 782, 844, 1367, 1964, 3055
BseRI GAGGAG 6 cut(s) 205, 1004, 1571, 1576, 1589, 1989
BseSI GKGCMC 2 cut(s) 382, 2362
BseX3I CGGCCG 1 cut(s) 174
BseXI GCAGC 7 cut(s) 1094, 1558, 2278, 2656, 3139, 3253, 3308
BseYI CCCAGC 2 cut(s) 241, 326
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 4 cut(s) 28, 176, 680, 1595
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 3 cut(s) 1105, 2362, 2905
BsiSI CCGG 2 cut(s) 56, 746
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 6 cut(s) 89, 108, 1801, 1970, 2462, 2726
BsmAI GTCTC 7 cut(s) 187, 213, 1123, 1662, 1904, 2039, 3002
BsmBI CGTCTC 2 cut(s) 1123, 1904
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 4 cut(s) 28, 176, 680, 1595
Bso31I GGTCTC 1 cut(s) 3002
Bsp119I TTCGAA 1 cut(s) 3043
Bsp1286I GDGCHC 4 cut(s) 382, 1105, 2362, 2905
Bsp143I GATC 8 cut(s) 691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 5 cut(s) 29, 1073, 2549, 2994, 3189
BspANI GGCC 4 cut(s) 28, 176, 680, 1595
BspHI TCATGA 1 cut(s) 1468
BspLI GGNNCC 5 cut(s) 766, 1793, 1806, 2211, 2604
BspMAI CTGCAG 1 cut(s) 2542
BspMI ACCTGC 2 cut(s) 2225, 2800
BspPI GGATC 4 cut(s) 1783, 2597, 2610, 2831
BspQI GCTCTTC 1 cut(s) 1047
BspT104I TTCGAA 1 cut(s) 3043
BspTNI GGTCTC 1 cut(s) 3002
BsrBI CCGCTC 1 cut(s) 31
BsrDI GCAATG 7 cut(s) 409, 745, 1808, 2116, 2338, 2809, 3021
BsrI ACTGG 8 cut(s) 94, 168, 315, 782, 844, 1367, 1964, 3055
BssECI CCNNGG 5 cut(s) 103, 1590, 2673, 2793, 3109
BssMI GATC 8 cut(s) 691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
BssNAI GTATAC 1 cut(s) 1675
BssNI GRCGYC 1 cut(s) 3105
BssSI CACGAG 1 cut(s) 2040
BssT1I CCWWGG 4 cut(s) 103, 1590, 2673, 2793
Bst1107I GTATAC 1 cut(s) 1675
Bst2BI CACGAG 1 cut(s) 2040
Bst2UI CCWGG 3 cut(s) 213, 1553, 3110
Bst6I CTCTTC 3 cut(s) 364, 390, 1047
BstACI GRCGYC 1 cut(s) 3105
BstAPI GCANNNNNTGC 2 cut(s) 1586, 2366
BstBAI YACGTR 1 cut(s) 1586
BstBI TTCGAA 1 cut(s) 3043
BstC8I GCNNGC 9 cut(s) 324, 467, 630, 1029, 1047, 1698, 2778, 2905, 3267
BstDSI CCRYGG 1 cut(s) 103
BstENI CCTNNNNNAGG 1 cut(s) 2724
BstF5I GGATG 6 cut(s) 311, 754, 1315, 1360, 1885, 2323
BstH2I RGCGCY 1 cut(s) 2456
BstHHI GCGC 2 cut(s) 2296, 2455
BstKTI GATC 8 cut(s) 694, 1122, 1778, 2386, 2605, 2716, 2839, 2899
BstMAI GTCTC 7 cut(s) 187, 213, 1123, 1662, 1904, 2039, 3002
BstMBI GATC 8 cut(s) 691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
BstMCI CGRYCG 1 cut(s) 177
BstNI CCWGG 3 cut(s) 213, 1553, 3110
BstNSI RCATGY 3 cut(s) 20, 2395, 2780
BstSCI CCNGG 4 cut(s) 55, 211, 1551, 3108
BstSFI CTRYAG 4 cut(s) 1173, 1482, 2538, 2721
BstSLI GKGCMC 2 cut(s) 382, 2362
BstV1I GCAGC 7 cut(s) 1094, 1558, 2278, 2656, 3139, 3253, 3308
BstV2I GAAGAC 3 cut(s) 269, 573, 1185
BstX2I RGATCY 2 cut(s) 2602, 2836
BstYI RGATCY 2 cut(s) 2602, 2836
BstZ17I GTATAC 1 cut(s) 1675
BstZI CGGCCG 1 cut(s) 174
Bsu36I CCTNAGG 2 cut(s) 675, 1758
BsuI GTATCC 2 cut(s) 2512, 2855
BsuRI GGCC 4 cut(s) 28, 176, 680, 1595
BtgI CCRYGG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 2068
BtsCI GGATG 6 cut(s) 311, 754, 1315, 1360, 1885, 2323
BtsIMutI CAGTG 5 cut(s) 322, 1677, 1860, 1971, 2930
BveI ACCTGC 2 cut(s) 2225, 2800
Cac8I GCNNGC 9 cut(s) 324, 467, 630, 1029, 1047, 1698, 2778, 2905, 3267
CaiI CAGNNNCTG 1 cut(s) 545
CciI TCATGA 1 cut(s) 1468
CfoI GCGC 2 cut(s) 2296, 2455
Cfr13I GGNCC 5 cut(s) 523, 958, 1253, 1427, 1805
CseI GACGC 1 cut(s) 244
Csp6I GTAC 2 cut(s) 1385, 2790
CviQI GTAC 2 cut(s) 1385, 2790
DpnI GATC 8 cut(s) 693, 1121, 1777, 2385, 2604, 2715, 2838, 2898
DpnII GATC 8 cut(s) 691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
DraI TTTAAA 2 cut(s) 1210, 2380
EaeI YGGCCR 1 cut(s) 174
EagI CGGCCG 1 cut(s) 174
Eam1104I CTCTTC 3 cut(s) 364, 390, 1047
EarI CTCTTC 3 cut(s) 364, 390, 1047
EclXI CGGCCG 1 cut(s) 174
Eco130I CCWWGG 4 cut(s) 103, 1590, 2673, 2793
Eco31I GGTCTC 1 cut(s) 3002
Eco32I GATATC 1 cut(s) 2332
Eco47I GGWCC 5 cut(s) 523, 958, 1253, 1427, 1805
Eco52I CGGCCG 1 cut(s) 174
Eco57I CTGAAG 3 cut(s) 375, 441, 784
Eco81I CCTNAGG 2 cut(s) 675, 1758
EcoNI CCTNNNNNAGG 1 cut(s) 2724
EcoO109I RGGNCCY 2 cut(s) 523, 1253
EcoRII CCWGG 3 cut(s) 211, 1551, 3108
EcoRV GATATC 1 cut(s) 2332
EcoT14I CCWWGG 4 cut(s) 103, 1590, 2673, 2793
EcoT22I ATGCAT 2 cut(s) 1885, 2397
ErhI CCWWGG 4 cut(s) 103, 1590, 2673, 2793
Esp3I CGTCTC 2 cut(s) 1123, 1904
FalI AAGNNNNNCTT 6 cut(s) 100, 132, 999, 1031, 2666, 2698
FaqI GGGAC 1 cut(s) 299
FbaI TGATCA 1 cut(s) 691
FblI GTMKAC 3 cut(s) 1674, 2070, 2199
Fnu4HI GCNGC 8 cut(s) 29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
FokI GGATG 6 cut(s) 298, 761, 1322, 1367, 1892, 2310
Fsp4HI GCNGC 8 cut(s) 29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
FspBI CTAG 4 cut(s) 252, 432, 2297, 2355
GlaI GCGC 2 cut(s) 2295, 2454
GluI GCNGC 8 cut(s) 29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
GsaI CCCAGC 2 cut(s) 245, 330
GsuI CTGGAG 3 cut(s) 1535, 2817, 3072
HaeII RGCGCY 1 cut(s) 2456
HaeIII GGCC 4 cut(s) 28, 176, 680, 1595
HapII CCGG 2 cut(s) 56, 746
HgaI GACGC 1 cut(s) 244
HhaI GCGC 2 cut(s) 2296, 2455
Hin1I GRCGYC 1 cut(s) 3105
Hin6I GCGC 2 cut(s) 2294, 2453
HinP1I GCGC 2 cut(s) 2294, 2453
HincII GTYRAC 5 cut(s) 361, 409, 558, 2071, 3082
HindII GTYRAC 5 cut(s) 361, 409, 558, 2071, 3082
HindIII AAGCTT 1 cut(s) 1246
HinfI GANTC 8 cut(s) 268, 449, 863, 1282, 1988, 2032, 2223, 2760
HpaI GTTAAC 1 cut(s) 558
HpaII CCGG 2 cut(s) 56, 746
Hpy99I CGWCG 1 cut(s) 2072
HpyCH4IV ACGT 4 cut(s) 1182, 1585, 2067, 3105
HpySE526I ACGT 4 cut(s) 1182, 1585, 2067, 3105
Hsp92I GRCGYC 1 cut(s) 3105
HspAI GCGC 2 cut(s) 2294, 2453
Ksp22I TGATCA 1 cut(s) 691
KspAI GTTAAC 1 cut(s) 558
Kzo9I GATC 8 cut(s) 691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
LguI GCTCTTC 1 cut(s) 1047
Lsp1109I GCAGC 7 cut(s) 1094, 1558, 2278, 2656, 3139, 3253, 3308
LweI GCATC 8 cut(s) 26, 75, 910, 1870, 2251, 2560, 3139, 3228
MaeI CTAG 4 cut(s) 252, 432, 2297, 2355
MaeII ACGT 4 cut(s) 1182, 1585, 2067, 3105
MaeIII GTNAC 7 cut(s) 1358, 1703, 1711, 1827, 1989, 2026, 2877
MalI GATC 8 cut(s) 693, 1121, 1777, 2385, 2604, 2715, 2838, 2898
MbiI CCGCTC 1 cut(s) 31
MboI GATC 8 cut(s) 691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
MfeI CAATTG 2 cut(s) 477, 1320
MflI RGATCY 2 cut(s) 2602, 2836
MhlI GDGCHC 4 cut(s) 382, 1105, 2362, 2905
MlyI GAGTC 3 cut(s) 872, 1997, 2041
MmeI TCCRAC 9 cut(s) 76, 103, 567, 764, 1283, 1769, 1857, 1987, 2529
Mph1103I ATGCAT 2 cut(s) 1885, 2397
MroXI GAANNNNTTC 1 cut(s) 112
MslI CAYNNNNRTG 6 cut(s) 704, 941, 1467, 1964, 2343, 3014
MspA1I CMGCKG 1 cut(s) 2711
MspI CCGG 2 cut(s) 56, 746
MspR9I CCNGG 4 cut(s) 57, 213, 1553, 3110
MunI CAATTG 2 cut(s) 477, 1320
MvaI CCWGG 3 cut(s) 213, 1553, 3110
NciI CCSGG 1 cut(s) 57
NcoI CCATGG 1 cut(s) 103
NdeII GATC 8 cut(s) 691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
NlaIV GGNNCC 5 cut(s) 766, 1793, 1806, 2211, 2604
NmuCI GTSAC 3 cut(s) 1358, 1711, 1989
NsiI ATGCAT 2 cut(s) 1885, 2397
NspI RCATGY 3 cut(s) 20, 2395, 2780
NspV TTCGAA 1 cut(s) 3043
PaeI GCATGC 1 cut(s) 2780
PagI TCATGA 1 cut(s) 1468
PciSI GCTCTTC 1 cut(s) 1047
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 5 cut(s) 268, 449, 1282, 2223, 2760
PflMI CCANNNNNTGG 1 cut(s) 1970
PfoI TCCNGGA 1 cut(s) 1551
PkrI GCNGC 8 cut(s) 30, 1084, 1548, 2293, 2671, 3154, 3243, 3298
PleI GAGTC 3 cut(s) 871, 1996, 2040
PpsI GAGTC 3 cut(s) 871, 1996, 2040
Ppu21I YACGTR 1 cut(s) 1586
PpuMI RGGWCCY 2 cut(s) 523, 1253
Psp5II RGGWCCY 2 cut(s) 523, 1253
Psp6I CCWGG 3 cut(s) 211, 1551, 3108
PspFI CCCAGC 2 cut(s) 241, 326
PspGI CCWGG 3 cut(s) 211, 1551, 3108
PspN4I GGNNCC 5 cut(s) 766, 1793, 1806, 2211, 2604
PspPI GGNCC 5 cut(s) 523, 958, 1253, 1427, 1805
PspPPI RGGWCCY 2 cut(s) 523, 1253
PstI CTGCAG 1 cut(s) 2542
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 2 cut(s) 2602, 2836
PvuII CAGCTG 1 cut(s) 2711
RsaI GTAC 2 cut(s) 1386, 2791
RsaNI GTAC 2 cut(s) 1385, 2790
RseI CAYNNNNRTG 6 cut(s) 704, 941, 1467, 1964, 2343, 3014
SalI GTCGAC 1 cut(s) 2069
SapI GCTCTTC 1 cut(s) 1047
SatI GCNGC 8 cut(s) 29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
Sau3AI GATC 8 cut(s) 691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
Sau96I GGNCC 5 cut(s) 523, 958, 1253, 1427, 1805
SchI GAGTC 3 cut(s) 872, 1997, 2041
ScrFI CCNGG 4 cut(s) 57, 213, 1553, 3110
SduI GDGCHC 4 cut(s) 382, 1105, 2362, 2905
SfaNI GCATC 8 cut(s) 26, 75, 910, 1870, 2251, 2560, 3139, 3228
SfcI CTRYAG 4 cut(s) 1173, 1482, 2538, 2721
SfuI TTCGAA 1 cut(s) 3043
SinI GGWCC 5 cut(s) 523, 958, 1253, 1427, 1805
SmiMI CAYNNNNRTG 6 cut(s) 704, 941, 1467, 1964, 2343, 3014
SmlI CTYRAG 4 cut(s) 646, 821, 986, 2441
SmoI CTYRAG 4 cut(s) 646, 821, 986, 2441
SpeI ACTAGT 1 cut(s) 431
SphI GCATGC 1 cut(s) 2780
SsiI CCGC 5 cut(s) 29, 1073, 2549, 2994, 3189
SspI AATATT 2 cut(s) 280, 663
SspMI CTAG 4 cut(s) 252, 432, 2297, 2355
StyD4I CCNGG 4 cut(s) 55, 211, 1551, 3108
StyI CCWWGG 4 cut(s) 103, 1590, 2673, 2793
TaiI ACGT 4 cut(s) 1185, 1588, 2070, 3108
TaqI TCGA 8 cut(s) 308, 444, 493, 2070, 3043, 3117, 3162, 3273
TaqII GACCGA 1 cut(s) 2185
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 5 cut(s) 268, 449, 1282, 2223, 2760
TscAI CASTG 5 cut(s) 322, 1684, 1867, 1971, 2937
TseFI GTSAC 3 cut(s) 1358, 1711, 1989
TseI GCWGC 7 cut(s) 1082, 1546, 2291, 2669, 3152, 3241, 3296
Tsp45I GTSAC 3 cut(s) 1358, 1711, 1989
TspGWI ACGGA 2 cut(s) 1863, 1928
TspRI CASTG 5 cut(s) 322, 1684, 1867, 1971, 2937
Van91I CCANNNNNTGG 1 cut(s) 1970
VneI GTGCAC 1 cut(s) 2358
VpaK11BI GGWCC 5 cut(s) 523, 958, 1253, 1427, 1805
XagI CCTNNNNNAGG 1 cut(s) 2724
XapI RAATTY 2 cut(s) 2300, 2863
XceI RCATGY 3 cut(s) 20, 2395, 2780
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
XmiI GTMKAC 3 cut(s) 1674, 2070, 2199
XmnI GAANNNNTTC 1 cut(s) 112
XspI CTAG 4 cut(s) 252, 432, 2297, 2355
ZraI GACGTC 1 cut(s) 3106
Zsp2I ATGCAT 2 cut(s) 1885, 2397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.