pycom05g33060

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004756
Physical Location & Seq
Reverse (-)
32228964 .. 32235995
7032 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGCTTCTTCCATCAAATATCACCGTTTTTACTCTTGATTTTTCGGGTTCAGGATTATCTGATGGTGATTATGTCAGCCTTGGTTGGCATGAGAGAGATGACCTTAAGATTGTGGTTTCATATTTAAGAAGCAAAAAGCAAAACTTGCGTATAGGTCTTTGGGGGCGATCGATGGGTGCAGTCACATGCCTACTTTATGGAGCAGAAGACCCTTCCATAGCCGGAATGGTGTTGGATAGTGCCTTTTCAAGCTTGTATGTTCTAATGATGGAACTAGTTGATGTATACAAGATTCGGCTTCCTAAATTTACTGTTAAGATGGCAATACAATATATGCGTCGGATAATTGAGAAGAAGGCAAAGTTTGATAGGATGGATCTTAATTGCTTGCAGGTTGCACCCAAAACATTCATTCCTGCTTTATTTGGACATGCTAAGGATGACAAATTCATCCAAACCCATCATTCTGACATCATCTACAAGTCTTATGCGGGGGACAAAAATATTATACATTTCGATGGTGATCACAACTCTTCTCGGCCACAATTTTATTATGATTCAGTTTCCATTTTCTTCTATAATGTTCTTCATCCCCCGCAAACGTCTTGTCATTCATGTAAGCTTGAGAAGTATTATGATTTGGGGGATTTGAAGATTGGTGCTGGTTTGGATGAGGGTTTGTTATATGAGATAATTACTGGAGTTCATTCTGCGGGTACTGACGTGGCAAGTTCATCTTCTGTTCCTCCTGCCATTTCAACCACAAAATGTGTTGGCGAACTTCTTTCTGAAATTGCACCAGTGACTGCTGCTGTTGATTCTATGCATGATGAAGCTGACACACTTAACAGTCACGAACCATCATATTTAGAGGAGCAGCGCAATGATGAGAATGTAGAATGTTGTTCATATACAAGCTCAAACAGGGAGAGTTGGGGCAGATGCTCTTCATTAGGAGGCAGTGATGAAGAATCTTCTGCTGCTTGCACAGCTGCTGACAGCAGTCATCAGAAGAACCTTAAGGTGTTTGCGATGCCTCTTGGATGCATACAACAAAAATCATCAGAACTAAGAAAAGAGGAAAAGAAAAAGAAGAAAGTTCCCATTGCTCCAAAGAAGCCCAGGGGTGAGAAATTTGAGAAGTTAGAGGCCCTTGGCAAACGACTGCGTCTTTGCATCCCAAAGCGAGTAAAAAAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

401

Amino Acids

44.63

Weight (kDa)

7.55

Isoelectric Point (pI)

53.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 6 - 88 1.6e-08 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 285
AciI CCGC 3 cut(s) 491, 596, 713
AclWI GGATC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 539
AcsI RAATTY 3 cut(s) 305, 446, 1133
AfaI GTAC 1 cut(s) 718
AflII CTTAAG 2 cut(s) 104, 1019
AgsI TTSAA 3 cut(s) 249, 652, 759
AhlI ACTAGT 1 cut(s) 274
AjiI CACGTC 1 cut(s) 724
AjnI CCWGG 1 cut(s) 1121
AluBI AGCT 5 cut(s) 252, 622, 836, 918, 992
AluI AGCT 5 cut(s) 252, 622, 836, 918, 992
AlwI GGATC 1 cut(s) 384
AlwNI CAGNNNCTG 2 cut(s) 806, 995
AoxI GGCC 2 cut(s) 539, 1149
ApeKI GCWGC 4 cut(s) 809, 877, 980, 992
ApoI RAATTY 3 cut(s) 305, 446, 1133
ArsI GACNNNNNNTTYG 4 cut(s) 592, 624, 1155, 1187
AspLEI GCGC 1 cut(s) 882
AspS9I GGNCC 1 cut(s) 1150
AsuHPI GGTGA 4 cut(s) 13, 77, 533, 1139
BaeI ACNNNNGTAYC 2 cut(s) 708, 741
BbsI GAAGAC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 796, 889, 967, 979
BccI CCATC 9 cut(s) 19, 56, 166, 262, 313, 367, 468, 512, 868
BciT130I CCWGG 1 cut(s) 1123
BclI TGATCA 1 cut(s) 523
BcuI ACTAGT 1 cut(s) 274
BfaI CTAG 1 cut(s) 275
BfrI CTTAAG 2 cut(s) 104, 1019
BfuAI ACCTGC 1 cut(s) 382
BisI GCNGC 4 cut(s) 810, 878, 981, 993
BlsI GCNGC 4 cut(s) 811, 879, 982, 994
Bme1390I CCNGG 1 cut(s) 1123
BmgBI CACGTC 1 cut(s) 724
BmgT120I GGNCC 1 cut(s) 1150
BmrFI CCNGG 1 cut(s) 1123
BmsI GCATC 4 cut(s) 932, 1023, 1034, 1185
BoxI GACNNNNGTC 1 cut(s) 1002
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 720
Bpu10I CCTNAGC 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 644
Bsa29I ATCGAT 1 cut(s) 170
BsaBI GATNNNNATC 1 cut(s) 522
BsaJI CCNNGG 4 cut(s) 79, 1121, 1122, 1153
Bse1I ACTGG 2 cut(s) 703, 800
Bse3DI GCAATG 2 cut(s) 889, 1104
Bse8I GATNNNNATC 1 cut(s) 522
BseBI CCWGG 1 cut(s) 1123
BseCI ATCGAT 1 cut(s) 170
BseDI CCNNGG 4 cut(s) 79, 1121, 1122, 1153
BseGI GGATG 7 cut(s) 378, 445, 450, 589, 676, 1049, 1176
BseJI GATNNNNATC 1 cut(s) 522
BseMI GCAATG 2 cut(s) 889, 1104
BseNI ACTGG 2 cut(s) 703, 800
BseRI GAGGAG 1 cut(s) 887
BseXI GCAGC 4 cut(s) 796, 889, 967, 979
BsgI GTGCAG 1 cut(s) 198
Bsh1285I CGRYCG 1 cut(s) 170
BshFI GGCC 2 cut(s) 541, 1151
BshVI ATCGAT 1 cut(s) 170
BsiEI CGRYCG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 509
BsnI GGCC 2 cut(s) 541, 1151
Bsp143I GATC 3 cut(s) 167, 376, 523
BspACI CCGC 3 cut(s) 491, 596, 713
BspANI GGCC 2 cut(s) 541, 1151
BspDI ATCGAT 1 cut(s) 170
BspMI ACCTGC 1 cut(s) 382
BspPI GGATC 1 cut(s) 384
BspQI GCTCTTC 1 cut(s) 952
BspTI CTTAAG 2 cut(s) 104, 1019
BsrDI GCAATG 2 cut(s) 889, 1104
BsrI ACTGG 2 cut(s) 703, 800
BssECI CCNNGG 4 cut(s) 79, 1121, 1122, 1153
BssMI GATC 3 cut(s) 167, 376, 523
BssNAI GTATAC 1 cut(s) 286
BssT1I CCWWGG 2 cut(s) 79, 1153
Bst1107I GTATAC 1 cut(s) 286
Bst2UI CCWGG 1 cut(s) 1123
Bst4CI ACNGT 3 cut(s) 25, 313, 851
Bst6I CTCTTC 2 cut(s) 538, 952
BstAFI CTTAAG 2 cut(s) 104, 1019
BstAPI GCANNNNNTGC 1 cut(s) 145
BstC8I GCNNGC 2 cut(s) 389, 985
BstDEI CTNAG 2 cut(s) 435, 1070
BstF5I GGATG 7 cut(s) 378, 445, 450, 589, 676, 1049, 1176
BstHHI GCGC 1 cut(s) 882
BstKTI GATC 3 cut(s) 170, 379, 526
BstMBI GATC 3 cut(s) 167, 376, 523
BstMCI CGRYCG 1 cut(s) 170
BstMWI GCNNNNNNNGC 2 cut(s) 145, 989
BstNI CCWGG 1 cut(s) 1123
BstNSI RCATGY 2 cut(s) 189, 434
BstPAI GACNNNNGTC 1 cut(s) 1002
BstSCI CCNGG 1 cut(s) 1121
BstV1I GCAGC 4 cut(s) 796, 889, 967, 979
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BstZ17I GTATAC 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 170
BsuRI GGCC 2 cut(s) 541, 1151
BsuTUI ATCGAT 1 cut(s) 170
BtgZI GCGATG 1 cut(s) 1046
BtrI CACGTC 1 cut(s) 724
BtsCI GGATG 7 cut(s) 378, 445, 450, 589, 676, 1049, 1176
BtsI GCAGTG 1 cut(s) 967
BtsIMutI CAGTG 2 cut(s) 807, 967
BveI ACCTGC 1 cut(s) 382
Cac8I GCNNGC 2 cut(s) 389, 985
CaiI CAGNNNCTG 2 cut(s) 806, 995
CfoI GCGC 1 cut(s) 882
Cfr13I GGNCC 1 cut(s) 1150
ClaI ATCGAT 1 cut(s) 170
CseI GACGC 2 cut(s) 326, 1157
Csp6I GTAC 1 cut(s) 717
CviAII CATG 5 cut(s) 89, 186, 431, 615, 827
CviQI GTAC 1 cut(s) 717
DdeI CTNAG 2 cut(s) 435, 1070
DpnI GATC 3 cut(s) 169, 378, 525
DpnII GATC 3 cut(s) 167, 376, 523
EaeI YGGCCR 1 cut(s) 539
Eam1104I CTCTTC 2 cut(s) 538, 952
EarI CTCTTC 2 cut(s) 538, 952
Eco130I CCWWGG 2 cut(s) 79, 1153
EcoO109I RGGNCCY 1 cut(s) 1150
EcoRII CCWGG 1 cut(s) 1121
EcoT14I CCWWGG 2 cut(s) 79, 1153
EcoT22I ATGCAT 2 cut(s) 828, 1049
ErhI CCWWGG 2 cut(s) 79, 1153
FaeI CATG 5 cut(s) 92, 189, 434, 618, 830
FalI AAGNNNNNCTT 4 cut(s) 128, 160, 721, 753
FaqI GGGAC 1 cut(s) 509
FatI CATG 5 cut(s) 88, 185, 430, 614, 826
FauI CCCGC 3 cut(s) 484, 603, 706
FbaI TGATCA 1 cut(s) 523
FblI GTMKAC 1 cut(s) 285
Fnu4HI GCNGC 4 cut(s) 810, 878, 981, 993
FokI GGATG 7 cut(s) 385, 437, 452, 576, 683, 1056, 1163
Fsp4HI GCNGC 4 cut(s) 810, 878, 981, 993
FspBI CTAG 1 cut(s) 275
GlaI GCGC 1 cut(s) 881
GluI GCNGC 4 cut(s) 810, 878, 981, 993
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 2 cut(s) 541, 1151
HapII CCGG 1 cut(s) 222
HgaI GACGC 2 cut(s) 326, 1157
HhaI GCGC 1 cut(s) 882
Hin1II CATG 5 cut(s) 92, 189, 434, 618, 830
Hin6I GCGC 1 cut(s) 880
HinP1I GCGC 1 cut(s) 880
HindIII AAGCTT 2 cut(s) 250, 620
HinfI GANTC 4 cut(s) 292, 557, 818, 971
HpaII CCGG 1 cut(s) 222
HphI GGTGA 4 cut(s) 13, 77, 533, 1139
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 6 cut(s) 61, 342, 469, 790, 1011, 1066
Hpy188III TCNNGA 3 cut(s) 35, 51, 854
Hpy8I GTNNAC 1 cut(s) 286
Hpy99I CGWCG 1 cut(s) 342
HpyAV CCTTC 2 cut(s) 222, 349
HpyCH4III ACNGT 3 cut(s) 25, 313, 851
HpyCH4IV ACGT 2 cut(s) 602, 723
HpyCH4V TGCA 8 cut(s) 179, 391, 398, 797, 826, 987, 1047, 1176
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 989
HpyF3I CTNAG 2 cut(s) 435, 1070
HpySE526I ACGT 2 cut(s) 602, 723
Hsp92II CATG 5 cut(s) 92, 189, 434, 618, 830
HspAI GCGC 1 cut(s) 880
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 3 cut(s) 167, 376, 523
LguI GCTCTTC 1 cut(s) 952
LmnI GCTCC 3 cut(s) 200, 874, 1114
Lsp1109I GCAGC 4 cut(s) 796, 889, 967, 979
LweI GCATC 4 cut(s) 932, 1023, 1034, 1185
MaeI CTAG 1 cut(s) 275
MaeII ACGT 2 cut(s) 602, 723
MaeIII GTNAC 3 cut(s) 181, 802, 851
MalI GATC 3 cut(s) 169, 378, 525
MboI GATC 3 cut(s) 167, 376, 523
MflI RGATCY 1 cut(s) 376
MluCI AATT 9 cut(s) 305, 345, 382, 446, 545, 693, 792, 1133, 1196
MmeI TCCRAC 2 cut(s) 213, 320
MnlI CCTC 7 cut(s) 667, 756, 865, 950, 1047, 1072, 1141
Mph1103I ATGCAT 2 cut(s) 828, 1049
MseI TTAA 6 cut(s) 105, 125, 315, 381, 846, 1020
MslI CAYNNNNRTG 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 992
MspCI CTTAAG 2 cut(s) 104, 1019
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 1123
MvaI CCWGG 1 cut(s) 1123
MwoI GCNNNNNNNGC 2 cut(s) 145, 989
NdeII GATC 3 cut(s) 167, 376, 523
NlaIII CATG 5 cut(s) 92, 189, 434, 618, 830
NmeAIII GCCGAG 1 cut(s) 517
NmuCI GTSAC 3 cut(s) 181, 802, 851
NsiI ATGCAT 2 cut(s) 828, 1049
NspI RCATGY 2 cut(s) 189, 434
PasI CCCWGGG 1 cut(s) 1122
PciSI GCTCTTC 1 cut(s) 952
PfeI GAWTC 4 cut(s) 292, 557, 818, 971
PflFI GACNNNGTC 1 cut(s) 1167
PkrI GCNGC 4 cut(s) 811, 879, 982, 994
Ple19I CGATCG 1 cut(s) 170
PshAI GACNNNNGTC 1 cut(s) 1002
Psp6I CCWGG 1 cut(s) 1121
PspGI CCWGG 1 cut(s) 1121
PspPI GGNCC 1 cut(s) 1150
PstNI CAGNNNCTG 2 cut(s) 806, 995
PsuI RGATCY 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 1167
PvuI CGATCG 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 1 cut(s) 718
RsaNI GTAC 1 cut(s) 717
RseI CAYNNNNRTG 1 cut(s) 516
SapI GCTCTTC 1 cut(s) 952
SaqAI TTAA 6 cut(s) 105, 125, 315, 381, 846, 1020
SatI GCNGC 4 cut(s) 810, 878, 981, 993
Sau3AI GATC 3 cut(s) 167, 376, 523
Sau96I GGNCC 1 cut(s) 1150
ScrFI CCNGG 1 cut(s) 1123
SfaNI GCATC 4 cut(s) 932, 1023, 1034, 1185
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 3 cut(s) 104, 623, 1019
SmoI CTYRAG 3 cut(s) 104, 623, 1019
SpeI ACTAGT 1 cut(s) 274
Sse9I AATT 9 cut(s) 305, 345, 382, 446, 545, 693, 792, 1133, 1196
SsiI CCGC 3 cut(s) 491, 596, 713
SspI AATATT 1 cut(s) 505
SspMI CTAG 1 cut(s) 275
StyD4I CCNGG 1 cut(s) 1121
StyI CCWWGG 2 cut(s) 79, 1153
TaaI ACNGT 3 cut(s) 25, 313, 851
TaiI ACGT 2 cut(s) 605, 726
TaqI TCGA 2 cut(s) 170, 516
TasI AATT 9 cut(s) 305, 345, 382, 446, 545, 693, 792, 1133, 1196
TfiI GAWTC 4 cut(s) 292, 557, 818, 971
Tru1I TTAA 6 cut(s) 105, 125, 315, 381, 846, 1020
Tru9I TTAA 6 cut(s) 105, 125, 315, 381, 846, 1020
TscAI CASTG 2 cut(s) 807, 967
TseFI GTSAC 3 cut(s) 181, 802, 851
TseI GCWGC 4 cut(s) 809, 877, 980, 992
Tsp45I GTSAC 3 cut(s) 181, 802, 851
TspRI CASTG 2 cut(s) 807, 967
Tth111I GACNNNGTC 1 cut(s) 1167
Vha464I CTTAAG 2 cut(s) 104, 1019
XapI RAATTY 3 cut(s) 305, 446, 1133
XceI RCATGY 2 cut(s) 189, 434
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 285
XspI CTAG 1 cut(s) 275
Zsp2I ATGCAT 2 cut(s) 828, 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.