pycom06g03550

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009459
Physical Location & Seq
Forward (+)
4459081 .. 4471209
12129 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4155 bp
ATGCCCACTATACGTGCCACGTCGTACCTCCAAGAGACGCACCCGGTCACGTGCCAGCGCGTCCCAAATATTGCAAAAAAAGTGGGTCCCATTTGGGCACTCGCCCAAAAACGAAACTTACAAAAACCCCCTCACACGGACTCTCTCTCTCTCTCTCCACGACGTCTTCACCCATCACACCTCCCACACTCTCTCTTCTTAAACGTCTTTCTTCTCTCTTCGCATCAATTTCCTTCACCAAACACTAACCCTAACCCTAACCCCCCTTCCACCCCCAAAAGATTTCAATTCCGCACCCCGACCATGGATTCGGAGCCCAACAACCTCTTTGACACGGCGTCGCAGCCCGACACCGCCAACGACGCCTACACCTTCCTCGAATTCAACACCCAGGGCGAGGACTTTGACTACCCGGAGTTCCGCGACCCGATTCGCTCTCCTGTCGCCTGGCCCACCCCTTCAGATTCCTTATCGGAACCTGCAGACCGTGACCGTGGCGGTGGTGGGGTCGGGTCCGACCACCAGTCCGATGCGTCGCCGGTTTCTGCTGCCCCGGGCAGCGCCACAAAGGCTAGGGCAGGTGGGTCGGGCAGTAACGCCGGCAATAATCAGGTGGTTGACGTCCTAACGCCTGGGATGAGTGTGCTTAACTTTGAGGATACTGGGGATGACGACAATTACGAGTTTGGAAAAGGGGATTTTACAGAACATGCGTGCCGGTACTGCGGCGTGTCGAACCCGGCTTGCGTTGTGAGGTGTAATGTGCCTTCGTGTCGAAAATGGTTCTGTAATTCAAGGGGGAACACTTCCGGCTCTCATATTGTGAATCACCTGGTCCGAGCAAAACACAAGGAAGTCTGTCTTCATAAAGATAGCCCATTGGGAGAAACAATCCTTGAATGCTACAACTGTGGATGTCGTAATGTATTTCTTCTTGGATTTATTTCAGCCAAGACAGAGAGTGTTGTTGTTCTTCTTTGTAGGGAACCCTGCTTAAGTGTTAATGCATTGAAGGATATGAAATGGATCGATCTGAGCCAGTGGTGTCCCCTGATAGATGATAGGTGTTTCTTGCAGTGGCTTGTTAAGGTCCCGTCTGAACAGGAGCAGCTAAGGGCGCGCCAAATCAGTGCTCAACAAATAAATAAGGTAGAAGAACTTTGGAAGTCCAATCCTGATGCTTCCCTTGAAGATCTCGAGAAGCCTGGTGTGGATGATGAACCCCAACCTGTGGCCTTGAAGTATGAAGATGCTTACCAGTATCAAAATGTGTTTGCACCACTTATTAAACTTGAAGCTGACTATGATAAAATGATGAAAGAATCTCAAAGCAAAGACAATGTCACAATTCGGTGGGATATTGGTCTTAACAAGAAGCGTATTGCTTATTTTGTGTTTCCAAAGGAAGACAATGAGTTACGTCTTGTACCTGGTGATGAGTTGCGGCTTCGTTATTCTGGAGATGCAGCTCATCCAGCATGGCAGTCAGTGGGACATGTTATCAAGTTAACTGCACAAGAGGAGGTTGCACTTGAACTTCGTGCCAGTCAGGGAGTTCCAGTTGATGTGAACCATGGCTTCAGTGTTGACTTTGTCTGGAAGAGTACAAGTTTTGATCGGATGCAGGGGGCCATGAAAGCTTTTGCAGTGGACGAGACAAGTGTTAGTGGATATATTTACCATCATTTGTTAGGCCATGAAGTAGAGGTTCAAATGGTCCGTAATACATTGCCCCGTCGTTTTGGTGCACCTGGTCTTCCGGAGCTCAATGCATCTCAAGTTTTTGCTGTAAAGAGTGTCCTTCAGAAGCCTATAAGTTTGATCGAGGGTCCACCTGGCACAGGAAAAACTGTGACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGCCAGGGACAGGTTTTGGTTTGTGCTCCTAGTAATGTTGCTGTAGACCAACTAGCTGAGAAGATAAGTGCAACTGGGTTGAAGGTTGTCAGACTTTGTGCAAAATCAAGGGAAGCTGTTAGTTCTCCTGTTGAGCATTTAACCCTTCATTACCAGGTTCGTCATCTTGACACGTCAGAAAAGAGTGAACTGTACAAGCTACAACAATTGAAGGATGAGCAAGGAGAATTGTCTAGTAGCGATGAGAAAAAATATAAGGCACTGAAGCGGGCAACCGAGAGGGAGATTTCTCAAAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGAGATCCACGATTGGCCAATTTTAGGTTCCGTCAGGTACTTATTGATGAGTCTACTCAGTCTACGGAGCCTGAATGCCTGATTCCTTTGGTTCTTGGGGCAAAGCAGGTTGTTCTTGTTGGAGATCACTGCCAGCTTGGGCCAGTCATTAAGTGCAAGAAAGCAGCTCGTGCTGGGCTTGCCCAGTCTCTCTTTGAACGCCTCGTTTTACTTGGTGTGAAACCAATTAGATTGCAGGTTCAATACCGAATGCATCCAGCCTTGTCAGAATTTCCATCTAATAGCTTCTATGAGGGCACGCTTCAAAATGGAGTCACCATCAATGAAAGGCAATCTTCAGGCATTGATTTTCCTTGGCCGGTTCCCAATCGTCCCATGTTCTTTTATGTACAGATGGGCCAAGAGGAGATAAGTGCTAGTGGAACATCCTATCTGAACAGAACTGAGGCTGCTAATGTTGAAAAGATTGTCACAACATTCTTGAGGAGTGGTGTAGTCCCTAGTCAGATTGGAGTGATCGCACCGTATGAGGGACAGAGAGCTTATATTGTGAACTATATGTCGCGAAATGGTGCTCTCAGACAGCAACTTTATAAGGAAATTGAGGTTGCAAGCGTAGATTCATTTCAGGGAAGGGAAAAGGATTATATCATCCTTTCGTGTGTGAGGAGTAATGAGCATCAGGGCATCGGATTCCTTAATGATCCTCGCAGGCTCAATGTTGCTCTAACACGTGCTCGATATGGTATTGTCATTCTTGGAAACCCTAAAGTTCTTAGTAAACAGCCACTGTGGAATAGCTTGTTGACACATTACAAGGAACACGAGTGCTTGGTTGAGGGGCCTCTAAATAACCTGAAGCAGAGTATGGTGCAATTTCCAAAGCCTAAAAAGATCTACAATGATCGGCGCCTATTCTTTGGAGGTGGTCCTGGAGTTATCCCCAATGACAGTTTTGGGTCTGTTGCCTCAGCTGGCCAAAATGCGGACAGAAGAAGTAATCGTGGTAGGGGTTCTTACCTACCTCCTGCCCCACCCAATGGTACCCATAAGCCTGGTGTACATCCTGCTGGATATCCAATGCCTCGGGCACCTCTTTCACCATTCCACGGAGGACCCCTTTCTCAACCGTATGCTATTCCAACTCGTGGAGCTGTGCATGGACCAGTTGGAGCTGTTCCTCATGTCCCTCAATCTGGAAGTCGTGGTTTTGGGGCAGGACGCGGAAATGCTGGTGCTCCAATTGGCAGTCATCTTCCGCACCAGCAAGGCACACAACAGAATGTTGGGAATATGGGGTCTACCTTCAACTTTCCTGCTCTTGAAAATCCAAATAGCCAACCATCTGTTGGTGGCCCCTTATCTCAACCTGGATTTGTCAATAATATGCCAGTTCAAGGGCCAAGCCAAACATTTCGTGATGGATTTTCCATGGCGGGCATGTCTCAGGAATTTTTGGGTGACGACTTTAAAAGTCAGGGATCACATGTTCCTTATAATGTTGCTGACTTCTCCACACAGGCTTCTCAAAGTGGATATGCTGTTGATTATGTTACTCAAGGAGCACAAGGTGGGTTTCCAGGGAACTTTCTGAACCAGAACTCTCAAGCTGGATATTCTCGATTTGGTACAGGGAATGACTTCATGTCTCAGGACTACATGCCTCATGGATCACAAGGCCTATTTACTCAGGTGGGCTTTAATGACCCATCACAGGATGAATGGGTTGGGCATATGACCAACATGTTTACTCTGATACGTTCAACATATTTTCAGGGCTTTATGAACTCGCTGTACTCTCAGCCGTTTGCCCATTACAACACACAGCCAATGAACTTGCAGGCCCCACCACAACAGCAGCAGCAGCAGCCACCGCAGCCTCAGCCTCAGCAGGGTCAGACCTCCCAAAATCAGAAAATCCACTACAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

1385

Amino Acids

152.51

Weight (kDa)

7.39

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF1_Zn_bind PF09416 237 - 391 3e-70 RNA helicase (UPF2 interacting domain)
UPF1_1B_dom PF18141 443 - 532 5.3e-27 RNA helicase UPF1, 1B domain
AAA_11 PF13086 588 - 709 1.4e-22 AAA domain
AAA_30 PF13604 588 - 790 2.7e-13 AAA domain
ResIII PF04851 588 - 658 8.9e-06 Type III restriction enzyme, res subunit
AAA_19 PF13245 595 - 789 9.1e-11 AAA domain
AAA_11 PF13086 690 - 791 3.8e-28 AAA domain
AAA_12 PF13087 800 - 996 4.7e-61 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2814, 3717
AarI CACCTGC 1 cut(s) 569
AatII GACGTC 2 cut(s) 166, 624
Acc36I ACCTGC 4 cut(s) 487, 569, 2317, 2446
Acc65I GGTACC 1 cut(s) 3263
AccB1I GGYRCC 3 cut(s) 3129, 3263, 3310
AccB7I CCANNNNNTGG 4 cut(s) 1231, 3260, 3368, 3569
AccI GTMKAC 4 cut(s) 1935, 2273, 2282, 3521
AccII CGCG 5 cut(s) 60, 423, 1120, 2785, 3444
AccIII TCCGGA 1 cut(s) 1759
AclWI GGATC 5 cut(s) 1034, 2219, 2918, 3709, 3898
AcoI YGGCCR 3 cut(s) 2235, 2576, 3196
AcsI RAATTY 3 cut(s) 380, 2489, 3671
AcuI CTGAAG 6 cut(s) 444, 1564, 1787, 2174, 2541, 3098
AcvI CACGTG 2 cut(s) 51, 2954
AcyI GRCGYC 5 cut(s) 163, 338, 363, 621, 3130
AdeI CACNNNGTG 1 cut(s) 3791
AflII CTTAAG 1 cut(s) 994
AflIII ACRYGT 6 cut(s) 1495, 2061, 2207, 2951, 3706, 3963
AhdI GACNNNNNGTC 3 cut(s) 337, 523, 3865
AjiI CACGTC 2 cut(s) 21, 2064
AjuI GAANNNNNNNTTGG 2 cut(s) 2231, 2263
AloI GAACNNNNNNTCC 2 cut(s) 3693, 3725
Alw21I GWGCWC 8 cut(s) 1135, 1750, 1767, 1918, 2797, 2959, 3460, 3787
Alw26I GTCTC 5 cut(s) 29, 1649, 2412, 3669, 3873
Alw44I GTGCAC 1 cut(s) 1746
AlwI GGATC 5 cut(s) 1034, 2219, 2918, 3709, 3898
AlwNI CAGNNNCTG 1 cut(s) 3010
Ama87I CYCGRG 3 cut(s) 553, 1196, 3306
Aor13HI TCCGGA 1 cut(s) 1759
ApaLI GTGCAC 1 cut(s) 1746
ApoI RAATTY 3 cut(s) 380, 2489, 3671
ArsI GACNNNNNNTTYG 4 cut(s) 292, 324, 1326, 1358
AscI GGCGCGCC 1 cut(s) 1118
Asp700I GAANNNNTTC 1 cut(s) 3860
Asp718I GGTACC 1 cut(s) 3263
AspLEI GCGC 5 cut(s) 60, 563, 1120, 1122, 3132
AsuC2I CCSGG 5 cut(s) 44, 413, 554, 555, 740
AsuHPI GGTGA 7 cut(s) 161, 228, 821, 1445, 2527, 3312, 3692
AvaI CYCGRG 3 cut(s) 553, 1196, 3306
AvaII GGWCC 9 cut(s) 86, 513, 835, 1090, 1717, 1829, 3149, 3335, 3383
BaeGI GKGCMC 4 cut(s) 100, 1750, 2519, 3313
BalI TGGCCA 2 cut(s) 2237, 3198
BanI GGYRCC 3 cut(s) 3129, 3263, 3310
BanII GRGCYC 2 cut(s) 318, 1767
BarI GAAGNNNNNNTAC 2 cut(s) 751, 783
BauI CACGAG 3 cut(s) 2388, 3044, 3366
BbrPI CACGTG 2 cut(s) 51, 2954
BbsI GAAGAC 4 cut(s) 158, 854, 1413, 1748
Bbv12I GWGCWC 8 cut(s) 1135, 1750, 1767, 1918, 2797, 2959, 3460, 3787
BbvCI CCTCAGC 3 cut(s) 3190, 4101, 4107
BccI CCATC 8 cut(s) 181, 1689, 2503, 2546, 2608, 3571, 3635, 3937
BceAI ACGGC 2 cut(s) 351, 4009
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciVI GTATCC 1 cut(s) 652
BcnI CCSGG 5 cut(s) 44, 413, 554, 555, 740
BcoDI GTCTC 5 cut(s) 29, 1649, 2412, 3669, 3873
BfaI CTAG 6 cut(s) 573, 1920, 1943, 2124, 2637, 2721
BfmI CTRYAG 3 cut(s) 480, 1860, 1932
BfoI RGCGCY 2 cut(s) 564, 3133
BfrI CTTAAG 1 cut(s) 994
BfuAI ACCTGC 4 cut(s) 487, 569, 2317, 2446
BfuI GTATCC 1 cut(s) 652
BglI GCCNNNNNGGC 1 cut(s) 569
BglII AGATCT 2 cut(s) 1192, 3114
Bme18I GGWCC 9 cut(s) 86, 513, 835, 1090, 1717, 1829, 3149, 3335, 3383
BmeRI GACNNNNNGTC 3 cut(s) 337, 523, 3865
BmeT110I CYCGRG 3 cut(s) 553, 1196, 3306
BmgBI CACGTC 2 cut(s) 21, 2064
BmrI ACTGGG 3 cut(s) 672, 1974, 2398
BmuI ACTGGG 3 cut(s) 672, 1974, 2398
BpiI GAAGAC 4 cut(s) 158, 854, 1413, 1748
BpmI CTGGAG 3 cut(s) 1479, 2241, 3174
Bpu10I CCTNAGC 4 cut(s) 1112, 3190, 4101, 4107
BpuEI CTTGAG 4 cut(s) 1761, 2722, 3762, 3810
BpuMI CCSGG 5 cut(s) 44, 413, 554, 555, 740
Bsa29I ATCGAT 1 cut(s) 1029
BsaAI YACGTR 3 cut(s) 14, 51, 2954
BsaHI GRCGYC 5 cut(s) 163, 338, 363, 621, 3130
BsaWI WCCGGW 1 cut(s) 1759
BsaXI ACNNNNNCTCC 2 cut(s) 1545, 1575
Bse118I RCCGGY 4 cut(s) 538, 599, 717, 2578
Bse3DI GCAATG 1 cut(s) 1727
BseAI TCCGGA 1 cut(s) 1759
BseCI ATCGAT 1 cut(s) 1029
BseMI GCAATG 1 cut(s) 1727
BsePI GCGCGC 1 cut(s) 1118
BseRI GAGGAG 4 cut(s) 1535, 2639, 2719, 2902
BseSI GKGCMC 4 cut(s) 100, 1750, 2519, 3313
BseYI CCCAGC 1 cut(s) 2393
BsgI GTGCAG 1 cut(s) 1497
Bsh1236I CGCG 5 cut(s) 60, 423, 1120, 2785, 3444
BshNI GGYRCC 3 cut(s) 3129, 3263, 3310
BshVI ATCGAT 1 cut(s) 1029
BsiHKAI GWGCWC 8 cut(s) 1135, 1750, 1767, 1918, 2797, 2959, 3460, 3787
BsiHKCI CYCGRG 3 cut(s) 553, 1196, 3306
BsmAI GTCTC 5 cut(s) 29, 1649, 2412, 3669, 3873
BsmBI CGTCTC 1 cut(s) 29
BsmI GAATGC 3 cut(s) 905, 2300, 2475
BsoBI CYCGRG 3 cut(s) 553, 1196, 3306
Bsp13I TCCGGA 1 cut(s) 1759
Bsp1407I TGTACA 3 cut(s) 2082, 2608, 3280
Bsp19I CCATGG 3 cut(s) 303, 1573, 3651
Bsp68I TCGCGA 1 cut(s) 2785
BspDI ATCGAT 1 cut(s) 1029
BspEI TCCGGA 1 cut(s) 1759
BspFNI CGCG 5 cut(s) 60, 423, 1120, 2785, 3444
BspMAI CTGCAG 2 cut(s) 484, 1864
BspMI ACCTGC 4 cut(s) 487, 569, 2317, 2446
BspPI GGATC 5 cut(s) 1034, 2219, 2918, 3709, 3898
BspT107I GGYRCC 3 cut(s) 3129, 3263, 3310
BspTI CTTAAG 1 cut(s) 994
BsrDI GCAATG 1 cut(s) 1727
BsrFI RCCGGY 4 cut(s) 538, 599, 717, 2578
BsrGI TGTACA 3 cut(s) 2082, 2608, 3280
BssAI RCCGGY 4 cut(s) 538, 599, 717, 2578
BssHII GCGCGC 1 cut(s) 1118
BssNI GRCGYC 5 cut(s) 163, 338, 363, 621, 3130
BssSI CACGAG 3 cut(s) 2388, 3044, 3366
BssT1I CCWWGG 4 cut(s) 303, 1573, 2573, 3651
Bst2BI CACGAG 3 cut(s) 2388, 3044, 3366
Bst4CI ACNGT 9 cut(s) 488, 494, 911, 1852, 2082, 2745, 3012, 3173, 3351
Bst6I CTCTTC 3 cut(s) 200, 223, 1595
BstACI GRCGYC 5 cut(s) 163, 338, 363, 621, 3130
BstAFI CTTAAG 1 cut(s) 994
BstAPI GCANNNNNTGC 1 cut(s) 2390
BstAUI TGTACA 3 cut(s) 2082, 2608, 3280
BstBAI YACGTR 3 cut(s) 14, 51, 2954
BstDSI CCRYGG 5 cut(s) 303, 493, 1573, 3328, 3651
BstENI CCTNNNNNAGG 1 cut(s) 1839
BstFNI CGCG 5 cut(s) 60, 423, 1120, 2785, 3444
BstH2I RGCGCY 2 cut(s) 564, 3133
BstHHI GCGC 5 cut(s) 60, 563, 1120, 1122, 3132
BstMAI GTCTC 5 cut(s) 29, 1649, 2412, 3669, 3873
BstNSI RCATGY 7 cut(s) 713, 1499, 2211, 3664, 3710, 3883, 3967
BstSFI CTRYAG 3 cut(s) 480, 1860, 1932
BstSLI GKGCMC 4 cut(s) 100, 1750, 2519, 3313
BstUI CGCG 5 cut(s) 60, 423, 1120, 2785, 3444
BstV2I GAAGAC 4 cut(s) 158, 854, 1413, 1748
BstX2I RGATCY 3 cut(s) 1192, 2224, 3114
BstXI CCANNNNNNTGG 2 cut(s) 3275, 4148
BstYI RGATCY 3 cut(s) 1192, 2224, 3114
Bsu15I ATCGAT 1 cut(s) 1029
BsuI GTATCC 1 cut(s) 652
BsuTUI ATCGAT 1 cut(s) 1029
BtgI CCRYGG 5 cut(s) 303, 493, 1573, 3328, 3651
BtgZI GCGATG 1 cut(s) 2145
BtrI CACGTC 2 cut(s) 21, 2064
BtsI GCAGTG 3 cut(s) 1082, 1653, 2347
BtsIMutI CAGTG 9 cut(s) 1046, 1082, 1135, 1494, 1588, 1653, 2150, 2347, 3008
BtuMI TCGCGA 1 cut(s) 2785
BveI ACCTGC 4 cut(s) 487, 569, 2317, 2446
CaiI CAGNNNCTG 1 cut(s) 3010
CfoI GCGC 5 cut(s) 60, 563, 1120, 1122, 3132
Cfr10I RCCGGY 4 cut(s) 538, 599, 717, 2578
Cfr9I CCCGGG 1 cut(s) 553
ClaI ATCGAT 1 cut(s) 1029
CseI GACGC 6 cut(s) 46, 49, 327, 371, 522, 3450
CsiI ACCWGGT 4 cut(s) 831, 1429, 1750, 2043
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
DinI GGCGCC 1 cut(s) 3131
DraI TTTAAA 1 cut(s) 3691
DraIII CACNNNGTG 1 cut(s) 3791
DriI GACNNNNNGTC 3 cut(s) 337, 523, 3865
EaeI YGGCCR 3 cut(s) 2235, 2576, 3196
Eam1104I CTCTTC 3 cut(s) 200, 223, 1595
Eam1105I GACNNNNNGTC 3 cut(s) 337, 523, 3865
EarI CTCTTC 3 cut(s) 200, 223, 1595
Ecl136II GAGCTC 1 cut(s) 1765
Eco130I CCWWGG 4 cut(s) 303, 1573, 2573, 3651
Eco147I AGGCCT 1 cut(s) 3900
Eco24I GRGCYC 2 cut(s) 318, 1767
Eco32I GATATC 1 cut(s) 3296
Eco47I GGWCC 9 cut(s) 86, 513, 835, 1090, 1717, 1829, 3149, 3335, 3383
Eco53kI GAGCTC 1 cut(s) 1765
Eco57I CTGAAG 6 cut(s) 444, 1564, 1787, 2174, 2541, 3098
Eco72I CACGTG 2 cut(s) 51, 2954
Eco88I CYCGRG 3 cut(s) 553, 1196, 3306
EcoICRI GAGCTC 1 cut(s) 1765
EcoNI CCTNNNNNAGG 1 cut(s) 1839
EcoO109I RGGNCCY 5 cut(s) 86, 1090, 3062, 3335, 4063
EcoRI GAATTC 1 cut(s) 380
EcoRV GATATC 1 cut(s) 3296
EcoT14I CCWWGG 4 cut(s) 303, 1573, 2573, 3651
EcoT22I ATGCAT 3 cut(s) 1009, 1774, 2475
EcoT38I GRGCYC 2 cut(s) 318, 1767
EgeI GGCGCC 1 cut(s) 3131
EheI GGCGCC 1 cut(s) 3131
ErhI CCWWGG 4 cut(s) 303, 1573, 2573, 3651
Esp3I CGTCTC 1 cut(s) 29
FalI AAGNNNNNCTT 4 cut(s) 846, 878, 2539, 2571
FauI CCCGC 2 cut(s) 2151, 3649
FauNDI CATATG 1 cut(s) 3954
FblI GTMKAC 4 cut(s) 1935, 2273, 2282, 3521
FriOI GRGCYC 2 cut(s) 318, 1767
FspBI CTAG 6 cut(s) 573, 1920, 1943, 2124, 2637, 2721
GlaI GCGC 5 cut(s) 59, 562, 1119, 1121, 3131
GsaI CCCAGC 1 cut(s) 2397
GsuI CTGGAG 3 cut(s) 1479, 2241, 3174
HaeII RGCGCY 2 cut(s) 564, 3133
HgaI GACGC 6 cut(s) 46, 49, 327, 371, 522, 3450
HhaI GCGC 5 cut(s) 60, 563, 1120, 1122, 3132
Hin1I GRCGYC 5 cut(s) 163, 338, 363, 621, 3130
Hin6I GCGC 5 cut(s) 58, 561, 1118, 1120, 3130
HinP1I GCGC 5 cut(s) 58, 561, 1118, 1120, 3130
HincII GTYRAC 4 cut(s) 619, 1509, 1588, 3027
HindII GTYRAC 4 cut(s) 619, 1509, 1588, 3027
HindIII AAGCTT 1 cut(s) 1638
HpaI GTTAAC 1 cut(s) 1509
HphI GGTGA 7 cut(s) 161, 228, 821, 1445, 2527, 3312, 3692
Hpy99I CGWCG 6 cut(s) 25, 165, 343, 365, 538, 1740
HpyCH4III ACNGT 9 cut(s) 488, 494, 911, 1852, 2082, 2745, 3012, 3173, 3351
Hsp92I GRCGYC 5 cut(s) 163, 338, 363, 621, 3130
HspAI GCGC 5 cut(s) 58, 561, 1118, 1120, 3130
KasI GGCGCC 1 cut(s) 3129
KflI GGGWCCC 1 cut(s) 86
Kpn2I TCCGGA 1 cut(s) 1759
KpnI GGTACC 1 cut(s) 3267
KroI GCCGGC 1 cut(s) 599
KroNI GCCGGC 1 cut(s) 601
KspAI GTTAAC 1 cut(s) 1509
LmnI GCTCC 9 cut(s) 313, 1105, 1762, 1921, 2287, 3371, 3392, 3463, 3782
MabI ACCWGGT 4 cut(s) 831, 1429, 1750, 2043
MaeI CTAG 6 cut(s) 573, 1920, 1943, 2124, 2637, 2721
MfeI CAATTG 3 cut(s) 1866, 2096, 3462
MflI RGATCY 3 cut(s) 1192, 2224, 3114
MlsI TGGCCA 2 cut(s) 2237, 3198
MluNI TGGCCA 2 cut(s) 2237, 3198
Mly113I GGCGCC 1 cut(s) 3130
MlyI GAGTC 3 cut(s) 134, 2279, 2541
MmeI TCCRAC 4 cut(s) 540, 2320, 3370, 3386
Mox20I TGGCCA 2 cut(s) 2237, 3198
Mph1103I ATGCAT 3 cut(s) 1009, 1774, 2475
MroI TCCGGA 1 cut(s) 1759
MroNI GCCGGC 1 cut(s) 599
MroXI GAANNNNTTC 1 cut(s) 3860
MscI TGGCCA 2 cut(s) 2237, 3198
MslI CAYNNNNRTG 1 cut(s) 4146
Msp20I TGGCCA 2 cut(s) 2237, 3198
MspA1I CMGCKG 1 cut(s) 3194
MspCI CTTAAG 1 cut(s) 994
MunI CAATTG 3 cut(s) 1866, 2096, 3462
Mva1269I GAATGC 3 cut(s) 905, 2300, 2475
MvnI CGCG 5 cut(s) 60, 423, 1120, 2785, 3444
NaeI GCCGGC 1 cut(s) 601
NarI GGCGCC 1 cut(s) 3130
NciI CCSGG 5 cut(s) 44, 413, 554, 555, 740
NcoI CCATGG 3 cut(s) 303, 1573, 3651
NdeI CATATG 1 cut(s) 3954
NgoMIV GCCGGC 1 cut(s) 599
NmuCI GTSAC 7 cut(s) 46, 488, 1342, 1852, 2533, 2689, 3680
NruI TCGCGA 1 cut(s) 2785
NsiI ATGCAT 3 cut(s) 1009, 1774, 2475
NspI RCATGY 7 cut(s) 713, 1499, 2211, 3664, 3710, 3883, 3967
PaeR7I CTCGAG 1 cut(s) 1196
PalAI GGCGCGCC 1 cut(s) 1118
PaqCI CACCTGC 1 cut(s) 569
PasI CCCWGGG 1 cut(s) 391
PauI GCGCGC 1 cut(s) 1118
PceI AGGCCT 1 cut(s) 3900
PciI ACATGT 4 cut(s) 1495, 2207, 3706, 3963
PcsI WCGNNNNNNNCGW 1 cut(s) 678
PctI GAATGC 3 cut(s) 905, 2300, 2475
PdiI GCCGGC 1 cut(s) 601
PdmI GAANNNNTTC 1 cut(s) 3860
PfeI GAWTC 8 cut(s) 308, 430, 464, 826, 1322, 2302, 2840, 2913
PflFI GACNNNGTC 2 cut(s) 1340, 1592
PflMI CCANNNNNTGG 4 cut(s) 1231, 3260, 3368, 3569
PfoI TCCNGGA 1 cut(s) 3151
PleI GAGTC 3 cut(s) 134, 2278, 2540
PluTI GGCGCC 1 cut(s) 3133
PmaCI CACGTG 2 cut(s) 51, 2954
PmlI CACGTG 2 cut(s) 51, 2954
PpsI GAGTC 3 cut(s) 134, 2278, 2540
Ppu21I YACGTR 3 cut(s) 14, 51, 2954
PpuMI RGGWCCY 3 cut(s) 86, 1090, 3335
PscI ACATGT 4 cut(s) 1495, 2207, 3706, 3963
PsiI TTATAA 2 cut(s) 2814, 3717
Psp124BI GAGCTC 1 cut(s) 1767
Psp5II RGGWCCY 3 cut(s) 86, 1090, 3335
PspCI CACGTG 2 cut(s) 51, 2954
PspFI CCCAGC 1 cut(s) 2393
PspPPI RGGWCCY 3 cut(s) 86, 1090, 3335
PsrI GAACNNNNNNTAC 2 cut(s) 3998, 4030
PstI CTGCAG 2 cut(s) 484, 1864
PstNI CAGNNNCTG 1 cut(s) 3010
PsuI RGATCY 3 cut(s) 1192, 2224, 3114
PsyI GACNNNGTC 2 cut(s) 1340, 1592
PteI GCGCGC 1 cut(s) 1118
PvuII CAGCTG 1 cut(s) 3194
RruI TCGCGA 1 cut(s) 2785
RseI CAYNNNNRTG 1 cut(s) 4146
SacI GAGCTC 1 cut(s) 1767
SchI GAGTC 3 cut(s) 134, 2279, 2541
SexAI ACCWGGT 4 cut(s) 831, 1429, 1750, 2043
SfcI CTRYAG 3 cut(s) 480, 1860, 1932
SfoI GGCGCC 1 cut(s) 3131
Sfr274I CTCGAG 1 cut(s) 1196
SgsI GGCGCGCC 1 cut(s) 1118
SinI GGWCC 9 cut(s) 86, 513, 835, 1090, 1717, 1829, 3149, 3335, 3383
SlaI CTCGAG 1 cut(s) 1196
SmaI CCCGGG 1 cut(s) 555
SmiMI CAYNNNNRTG 1 cut(s) 4146
SmlI CTYRAG 6 cut(s) 994, 1196, 1776, 2701, 3777, 3825
SmoI CTYRAG 6 cut(s) 994, 1196, 1776, 2701, 3777, 3825
SseBI AGGCCT 1 cut(s) 3900
SspDI GGCGCC 1 cut(s) 3129
SspI AATATT 1 cut(s) 70
SspMI CTAG 6 cut(s) 573, 1920, 1943, 2124, 2637, 2721
SstI GAGCTC 1 cut(s) 1767
StuI AGGCCT 1 cut(s) 3900
StyI CCWWGG 4 cut(s) 303, 1573, 2573, 3651
TaaI ACNGT 9 cut(s) 488, 494, 911, 1852, 2082, 2745, 3012, 3173, 3351
TaqI TCGA 8 cut(s) 378, 734, 775, 1029, 1197, 1824, 2959, 3841
TatI WGTACW 5 cut(s) 1604, 2082, 2608, 3280, 4014
TauI GCSGC 2 cut(s) 729, 1447
TfiI GAWTC 8 cut(s) 308, 430, 464, 826, 1322, 2302, 2840, 2913
TscAI CASTG 9 cut(s) 1046, 1082, 1135, 1494, 1588, 1653, 2157, 2354, 3015
TseFI GTSAC 7 cut(s) 46, 488, 1342, 1852, 2533, 2689, 3680
Tsp45I GTSAC 7 cut(s) 46, 488, 1342, 1852, 2533, 2689, 3680
TspGWI ACGGA 5 cut(s) 152, 1709, 2240, 2300, 3345
TspMI CCCGGG 1 cut(s) 553
TspRI CASTG 9 cut(s) 1046, 1082, 1135, 1494, 1588, 1653, 2157, 2354, 3015
Tth111I GACNNNGTC 2 cut(s) 1340, 1592
Van91I CCANNNNNTGG 4 cut(s) 1231, 3260, 3368, 3569
Vha464I CTTAAG 1 cut(s) 994
VneI GTGCAC 1 cut(s) 1746
VpaK11BI GGWCC 9 cut(s) 86, 513, 835, 1090, 1717, 1829, 3149, 3335, 3383
XagI CCTNNNNNAGG 1 cut(s) 1839
XapI RAATTY 3 cut(s) 380, 2489, 3671
XceI RCATGY 7 cut(s) 713, 1499, 2211, 3664, 3710, 3883, 3967
XcmI CCANNNNNNNNNTGG 1 cut(s) 3566
XhoI CTCGAG 1 cut(s) 1196
XmaI CCCGGG 1 cut(s) 553
XmiI GTMKAC 4 cut(s) 1935, 2273, 2282, 3521
XmnI GAANNNNTTC 1 cut(s) 3860
XspI CTAG 6 cut(s) 573, 1920, 1943, 2124, 2637, 2721
ZraI GACGTC 2 cut(s) 164, 622
Zsp2I ATGCAT 3 cut(s) 1009, 1774, 2475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.