pycom06g03680

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004352
Physical Location & Seq
Forward (+)
4638245 .. 4661477
23233 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 7275 bp
ATGCTTTCCCGCCACGTAAGATCGCAGAACCCTTCCGCACACACGGCTACATTGCACTGGTCAGACCACAATAACAATCTGTTCCGCCGTCGTCTGGAAGCCAACACTTTGCGATTTCTTCCGATTTCAGTCGGTCGAAAGGAGATAACAATTTGGGAGGAAGATCGGGCCGGCGAAGAGAAGATGTTGGAGGATCAGGTGGCGAACCTCCTGCAGAGGTACTTGGGGGACTACGTGAGGGGGCTCAACAAGGAGGCCCTCAAGATCAGTGTCTGGCGAGGTGATGTGGAGCTAAAAAATATGCAGCTGAAGCCTGAGGCACTCAATGCACTAAAATTACCAGTGAAGGTTAAGGCAGGATTTCTTGGATCAGTGAAACTTAAGGTTCCATGGAGCAAGCTTGGCCAGGATCCCGTTTTAGTTTCTTTGGACCGCATTTTTCTGTTAGCTGAACCTGAAACTCAAGTTGAAGGATCCAGTGAGGATGCTGTACAGGAAGCTAAGAAAAACCGAATTCGGGAAATGGAAATGAAGCTGTTAGAGAGGACACAGCAACTAAAGTCAGAAATGAATACGTCATGGTTAGGATCCCTGATCAGCACAATAATTGGAAATTTGAAACTCTCAATTTCAAATATTCACATCAGATATGAGGATATTGAAAGCAACCCGGGCCATCCGTTTGCAGCAGGCATAACCTTGGAAAGCCTTTCCGCTATGACAGTTGATAGCAAAGGAAATGAAACATTTGTCACTGGGGGTGCTCTAGACCGTACACAGAAGTCTGTTCAACTCGATCGGCTTGCACTTTATTTGGATTCTGATATTGCTCCATGGCATGTTAACAAACCATGGGAGGATTTGCTTCCATCAGAGTGGGTCCAGGTTTTTAGATTTGGTACTCAATATGGTAAACCAGCGGATAGATTACCCAAAAGGCACACTTACATACTGGAACCTGTGTCTGGAAATGCAAAGTACTCAAAACTACCGCCAAATGAATTTGCTGATAGTGGCCAGCCTTTGCACAAAGCAGCAGTTAATTTGGATGATGTGACTCTCTGTTTGCCAAAGGATGGATACAGGGATGCACTAAAGTTAGCAGATAATATTGCTGCATTTAATCAGCGATTGAAGTATGCTCATTATCGTCCACATGTCTCTGTAAAATCTGACCCTAGATCTTGGTGGAAGTATGCTTATAGAGTTGTTGCTGACCAAATGAAGAAGGCAAGTGGGAGATTGTCATGGGACCATGTTCTGAAGTATGCAAGCTTGCGTAAAAGATATATCTCTCTATATGCTTCACTTCTTAGATCTGATCCTCATCGGTTAGTAGTTGATGACAACCAAGATATCGAGGAGCTAGATCGTGGGCTTGATGTTGAACTGATAGTCCAGTGGAGGATGTTGGCTCACAAGTTTGTAGAGCAGTCATTACAGTCGGATCTTGACTCGAGGAAACAGAAAGCAAAGAAACCTTGGTGGGGATGGGGGAGTCGGTCTAAGATAGGTGGGAGTGAGCCAATTAGTTTCAGTGAGGAAGACTGGAAGCAGTTAAGTAGCATCATTGGCTATAAGGAAGGTGATGATAGTCTCTCAGTTATAAGTAATGACAAGGTAGATGCTCTTCAGACATCCTTGAGCATTTGCATGAAACATAATGCTACTAAGCTTATTGATGAGTCCCTGGAGTGTCTTGCTGAGTTATCATGTGAAGGCTTGGATTGCTTTATAAAGCTTTATCCAGAGGCAAAAGTTTTTGACATGAAATTAGGATCATACAAGTTGTCCACGCCGAGTGGTCTCCTTGCAGAGAGTGCGAATGCCTATGATTCTTTAGTTGGCACGTTCTGTTATAGGCCTTTCAACAAAAATGTGGACTGGAGCTTGGTTGCTAAAGCTTCTCCTTGTTATGTGACATATCTAAAGGATGCAATCGATCAAATTATCAAATTCTTTCGTAGCAGTACAGCTGTCAGTCAGACAATAGCATTGGAAACTGCAGCTGCAGTGCAGATGACAATCAATGGAGTCAAGCGCACAGCTCAGCAACAAGTGAATAGAGCATTGAAGGACCATTCCAGGTTTTTGTTGGACTTGGATATTGCAGCCCCAAAGATTACAATTCCTACTGATTTTTGTCCCGACAACACGCATCCAACAAAGCTCATGCTTGACTTGGGAAATTTGGTCATTGTTACCAAGGATAGCTATGAAGATGGATCACAGGAGGTGCTAGATTTGTATCTGCAGTTTAATTTAGTTTTAAGCGACGTATCTGCTTTTCTGGTTGATGGTGACTACTGTTGGAGTCAATCTCCTTCAAAGATGTCTTCTGGTTCTTCCAACTCAAATGGTGTTAGTTTGTTGCCTTTTATTGATAAATGTGGGGTCAACGTAAAGCTTGAACAGATAAGATTAGAGAGCCCATCCTACCCATCAACAAGAGTCGCTGTAAGGCTGCCATCATTACGGTTTCACTTCTCTCCAGCTCGGTATCATCGATTGATGCAAATCGTTAAGATGTTTGAGGAAGAGGAGGATAGTGAGGACTCAGGTCTTCTTTGCCCTTGGAATGAGGCTGACTTCGAGGGGTGGTTGTCTCTACTTGCGCGGAAGGGTCTTGGAAATAGGGAAGCAGTGTGGCAGCGGCGCTACCTATGCTTAGTTGGGCCTTTTCTTTATGTACTTGACAGCCCTACCTCTAAATCGTACAAACTGTGTATCAGCTTAAGTGGAAAACACATATATCAGGTTCCTCCAGAGTCTGTTGGTGGTGCTGATCTTGTTTTGGCTATCTGTGATGCTGCACGAGCAAATATCAAAGTGGTGGAGGATGCAAATGCACTAATATTGCAATGTGACTCCAACGATTCAAAGAAAACTTGGCAGAGCCGGTTGCAGGGGGCTGTCTATCGTTCATCGGGTTCTGCTCCTGTGACTAGTCTAACTGAAACTTCGTCGGAGTCTGAGGATTCAGTAACGGAGCTTAATTCCAATGAGGATCCGGTTGATTTATCAAAAATGGAGAGAGCCTTTATTACTGGTGTTCTTGATGAACTGAAGGTCTGCTTCAGTTACAGTAGTCAGCATGACCAGAATTTTATGAAAGTGCTCCTAACTGAAGAAAGGCGTTTATTTGAATTTCGAGCAATAGGTGGCCAGGTTGAGCTATCGGTTAGAGCAAGTGACATGTTCATTGGAACTGTTTTGAAATCTTTGGAGATTGAAGATTTAGTTTCTGGTCACAGGATGTCTCAGCCTCGCTATCTGGCAAGATCATTTACCCACAGTGCAGAAACAAATTTAACCTCTGGTGCCACCGGTAATAAAAGTTTTGATGGCAGTGTTGTAATCCCGAATGGAGATGAATTCTACGAGGCACCAGAAAATTTGGTTGACTCTGAAACTTTATTGTTGAAATCCCCTCGTTTTACCCGTATTGCTGGTTTGCTCCCTGGTCTTCAAGCAACAAAGGACATTGAACTCAAAGATCCGTTGGATAGTTTTGTGAAAGCTCAAATAGTTATCTATGACCAGAACTCTCCTCTGTACCATAACATAGACATGCTGGTCTCGGTCACCCTGGCTACCCTATCTTTTTTCTGTCGCCGACCAACAATTCTTGCCATCATGGAATTCGTTAATGCTATTAAGATGAAGGATGAAAGATGTGAATCTTTCAGTGACAGTTCTTCAGCACCTATTGTGAAACAGGACATCTCCAGAGATGATGCAGTTGATGGTCCACGATCAGTTACCATCAATGAGCCATCTATTAAAGGATTGCTGGGAAAGGGGAAGTCCAGGGTCGTTTTTAATTTAACATTAAACATGGCCCGTGCTCAAATTATATTAATGAATGAAGATGAAAGCAAGTTTGCTGTTTTGTCTCAAGATAATTTGGTTACCGATATTAAGGTCTTTCCATCCTCTTTTAGTATTAAGGCAGCCCTTGGAAATCTTAGAATAAGTGATGAGAGTCTTCCTAGCAGCCATATGTATTTCTGGGCATGTGACATGAGAAATCCAGGAGGCAGTTCATTTGTTGAGTTGGTTTTTACTTCATTCAGTGTTGATGATGAAGACTACGAAGGTTATGAGTATAGCCTTTTTGGGCAGCTTTCAGAAGTACGCATTGTTTATTTAAATCGGTTTATCCAGGAGGTGGCTAGTTACTTCATGGGTCTGGTTCCCAAGAATCCCAACAATCCGAAAGGTGTTGTCAAGCTAAAGGATCAAGTCACAAATTCTGAGAAATTGTTTACAACTAGTGATTTTGAGGGATCGCCTGCACTGAAGTTGGATGTATCTCTTAGGAAGCCTATCATTTTGATGCCTCGGAAAACAGACAGTCCTGACTACTTGAAGCTTGACATTGTTCATATTACTGTCCGGAACACCTTTAAATGGTTTGGTGGAAGTAGAAGTGAAATTAATGCTGTTCATATGGAAGTGTTAACAGTTCAGGTTGAGGACATCAATCTAAATGTTGGCACAAAAGCGGATTTAGGTGAAAGTATAATTCAAGACGTGAAGGGAGTTTCCGTTGTGATTCAAAGGTCACTTCGAGATTTGTTGCATCAAGTACCAAGTATTGAGGCTGCAATCAAGATGGAGAAATTGAAAGCAGCTTTGTCAAACAGGGAATATCAGATTATCACTGATTGTGCGCAGTCTAATATTTCTGAAACTCCACATGTTATACCTCCACTGAACCACGAGTCTACGATATCATCAGTTGATGTTGAAGAAGACATCACTCCTCAAGAGCCTGTTGTACTCGAATCTCGAAATGCAAATGAGGGAGCTTGGGTTATGATGAAAGTCTCTCTTGTTATTGGTTTGGTTGAATTATGCTTGCATACTGGAGTAGCAAGAGATGCACCTCTAGCCACTGTACAGATCAGTGGTGCATGGTTACTGTACAAGTCAAATACACTAGGAGAAGGCTTCCTATCAGCAACACTAAAGGGGTTTGCTGTGCTCGATGATCGTGAGGGTACTGAACCAGAGTTTAGGTTGGCAGTAGGAAAGCCTGAGTATGTTGGTTCAAGACCCCTAGATTTTGTGACTCATGATGGTGCTCAACAGATCTCCGGTGCACATGATACAGAAGTGAATGATTTCAAATTAGTTCCTACGATGCTCATTCTTGATGCAAAATTTTGTCAGCTGTCAACGGTTGTTTCTTTATGCATCCAAAGGCCACAACTGCTGGTTGTACTTGATTTTTTGCTGGGTGTTGTTGAGTTTTTTGTTCCTACAATCAGCAATACTCTTTCAAGTGAAGAAGTACAGAACTCCGTCCATGGAATGGATGCAGTGATCTTGGATCAGTCAACTTACAAGCAACCCTCTACTGAATTTTCTCTTTCTCCTCTAAGGCCTTTAATAGCTGATGATGGAAGGCACGGTCATTTTGTTTATGATGGCAATGGTGGTACACTATATTTGAAAGATAGGCAGGGAGCTATTCTCTCCGGCCCTAGTACAGAGCCAATAATATATGTAGGAGACGGGAAGAGGTTGCAATTCACAAATGTTGTCATCAAGAATGGACTGTACCTAGATTCATGCATTTCTATGGGATCCAACAGTAGCTATTCAGCATTGAAGGAAGATCAGGTCTCCTTTGTGGGAGGGAATGAAGATTCAAGTATGAATTCCTCAACAGAAAATGTCAATAATGTGCCACCTCAAAGTATTGTGGCCGACAAGTCCACAGAGATTGTTATTGAGTTGCAGTTGGTTGGCCCAGAGCTGACATTCTATAATACTTCTGAAGATGTTGGGGAATCACTTGTGCTCTCTAACCAACTTTTGCATGCACAGTTGGATGGATTCTGCAGGCTTGTTATGAAGGGTGATACCAAGGAGATGAATGCAAATGTGCTTGGTTTAACTATGGAGAGTAATGGAATCACGATTCTGGAGCCCTTCGACGCCTCTGTGAAATATTCAAATGCTTCTGGAAAGACCAATATACATCTAAGTGTTTCAGATATATTTATGAATTTTTCATTCAGCATCCTCAGACTGTTTTTAGCAGTTGAAGAGGATATATTGGCATTTTTAAGGACAACATCAAAGAAAATGACAGTGCTTTGCTCGCAGTTTGACAAAATTGGAACAATACGAGATCCCTTTGGTGACCAAATATATACTTTCTGGAGGCCTCAAGCACCTACTGGTTTTGCAGTTCTTGGGGATTACCTGACACCTTTGGACAAGCCGCCTACAAAAGCAGTTCTTGCTGTAAATACCAATTTTGCTAGAGTCAAGAAGCCCCTGTCTTTTAAGCTGATTTGGCCACCTTTACCTTCAGAGGGCTTGTCTGTCGATAGCGTAAATTATTCTGATTCACTACCAAATGATGTCCTTTCTGACGGAGCTAGTTGTTCCATTTGGTTCCCTGAAGCACCTAAGGGATATGTTGCATTGGGCTGTGTCGTTTCCCCTGGAAGAACACAGCCACCACTGTCTTCTGCTTTCTGTATCTTGGCTTCTTTAGTTTCTTCTTCTTCCTTAAGGGATTGCATTGCAATCAGTACCAACAATCCGTATCAGTCAACTTTAGCATTCTGGCGTGTGGATAATTCTGTTGGCACCTTTTTGCCAGCAGGTCCAAATATGTCTAGTGTAATGGGTACAGCCTATGATTTGCGTCACGTGATATTTGGATTTTCAGAAGCGTCTGAAAAATCATCTAATCGTTTCACTGTCCAAGATACTTCTTCTCAACATCATGATGTCCAATCAGAAAGGTTGGCAACCGTAAACTCTGGGCCGCATTATGAAGCTGTTGCTAGTTTCCAATTGATATGGTGGAATCAGGGCTCAAACTCAAGGAAGAAACTGTCTATATGGCGTCCAGTTGTCCCTCATGGGATGGTATATGTTGGTGATATTGCAATCAACGGGTACGAGCCCCCAAATACCTGCATTGTTCTTCATGAAACAGGAGATGGCGGGATCTTCAAAGCCCCTCTTGATTTCCAGCTTCTTGGGCAAGTTAAGAAACAGTGGGGAATGGACAGTATATCTTTCTGGCTTCCTCAGGCCCCTCCAGGGTTTGTTTCCCTTGGTTGTATTGCATGCAAGGGAACACCAAAACAATCTGATTTCAGGTCATTAAGATGCATGCGCAGTGATATGGTAGCTGGATCGATCAATTTTCAGAGGAAAGTGTATGGGATACATCTGATGCGAAGCGTACAAGAGATTCTTTTAGTATCTGGTCAGCTGGCAATGAGTTGGGGACCTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N PH Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

2425

Amino Acids

269.34

Weight (kDa)

5.56

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 62 - 544 6.6e-98 VPS13-like family N-terminal region
ATG2_CAD PF13329 70 - 187 1.1e-07 Autophagy-related protein 2
VPS13_N PF12624 617 - 738 4.1e-12 VPS13-like family N-terminal region
PH PF00169 864 - 972 1.5e-08 PH domain
VPS13_M PF25033 1430 - 2034 3.6e-12 VPS13-like family middle region
Vps62 PF06101 2039 - 2198 4.3e-10 Vacuolar protein sorting-associated protein 62
Vps62 PF06101 2269 - 2389 3.5e-07 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1607, 1736
AasI GACNNNNNNGTC 1 cut(s) 3575
Acc16I TGCGCA 2 cut(s) 4677, 7151
Acc36I ACCTGC 2 cut(s) 6617, 6953
AccB1I GGYRCC 3 cut(s) 3320, 3385, 6611
AccB7I CCANNNNNTGG 3 cut(s) 1076, 4171, 5320
AccI GTMKAC 1 cut(s) 4730
AccII CGCG 1 cut(s) 2617
AccIII TCCGGA 1 cut(s) 4398
AcoI YGGCCR 5 cut(s) 403, 1015, 3163, 5715, 6314
AcvI CACGTG 1 cut(s) 6676
AcyI GRCGYC 2 cut(s) 5949, 6874
AflII CTTAAG 3 cut(s) 380, 2734, 6532
AflIII ACRYGT 3 cut(s) 1156, 3195, 4702
AgeI ACCGGT 1 cut(s) 3326
AhlI ACTAGT 2 cut(s) 2945, 4274
AjiI CACGTC 1 cut(s) 4537
AloI GAACNNNNNNTCC 2 cut(s) 1380, 1412
Alw21I GWGCWC 7 cut(s) 766, 3120, 3850, 4992, 5092, 5110, 5814
Alw44I GTGCAC 1 cut(s) 5106
AlwNI CAGNNNCTG 2 cut(s) 273, 2771
Ama87I CYCGRG 2 cut(s) 670, 1456
Aor13HI TCCGGA 1 cut(s) 4398
ApaLI GTGCAC 1 cut(s) 5106
ArsI GACNNNNNNTTYG 2 cut(s) 1048, 1080
AseI ATTAAT 2 cut(s) 3860, 4440
AsiGI ACCGGT 1 cut(s) 3326
Asp700I GAANNNNTTC 1 cut(s) 5129
AspLEI GCGC 5 cut(s) 2043, 2617, 2658, 4678, 7152
AsuC2I CCSGG 2 cut(s) 671, 672
AsuHPI GGTGA 8 cut(s) 293, 1598, 2312, 3577, 4529, 5882, 6167, 6920
AvaI CYCGRG 2 cut(s) 670, 1456
AvaII GGWCC 7 cut(s) 430, 880, 1252, 2077, 3749, 6629, 7265
AxyI CCTNAGG 3 cut(s) 315, 6429, 7062
BaeGI GKGCMC 1 cut(s) 5110
BaeI ACNNNNGTAYC 2 cut(s) 5106, 5139
BalI TGGCCA 4 cut(s) 405, 1017, 3165, 6316
BamHI GGATCC 5 cut(s) 409, 473, 587, 3007, 5594
BanI GGYRCC 3 cut(s) 3320, 3385, 6611
BanII GRGCYC 5 cut(s) 246, 2432, 5943, 6845, 6936
BarI GAAGNNNNNNTAC 4 cut(s) 1614, 1646, 6493, 6525
BauI CACGAG 2 cut(s) 2814, 4724
BbrPI CACGTG 1 cut(s) 6676
BbsI GAAGAC 8 cut(s) 1551, 2328, 2555, 3458, 3980, 4095, 4764, 6480
Bbv12I GWGCWC 7 cut(s) 766, 3120, 3850, 4992, 5092, 5110, 5814
BceAI ACGGC 2 cut(s) 60, 72
BcgI CGANNNNNNTGC 6 cut(s) 193, 227, 785, 819, 2264, 2298
BciVI GTATCC 2 cut(s) 1073, 7194
BclI TGATCA 1 cut(s) 594
BcnI CCSGG 2 cut(s) 671, 672
BcuI ACTAGT 2 cut(s) 2945, 4274
BfmI CTRYAG 5 cut(s) 212, 2004, 2010, 2252, 5851
BfoI RGCGCY 1 cut(s) 2659
BfrI CTTAAG 3 cut(s) 380, 2734, 6532
BfuAI ACCTGC 2 cut(s) 6617, 6953
BfuI GTATCC 2 cut(s) 1073, 7194
BglII AGATCT 3 cut(s) 1181, 1316, 5097
BlpI GCTNAGC 1 cut(s) 2049
BmcAI AGTACT 1 cut(s) 980
Bme18I GGWCC 7 cut(s) 430, 880, 1252, 2077, 3749, 6629, 7265
BmeT110I CYCGRG 2 cut(s) 670, 1456
BmgBI CACGTC 1 cut(s) 4537
BmrI ACTGGG 1 cut(s) 765
BmuI ACTGGG 1 cut(s) 765
BoxI GACNNNNGTC 1 cut(s) 2559
BpiI GAAGAC 8 cut(s) 1551, 2328, 2555, 3458, 3980, 4095, 4764, 6480
BplI GAGNNNNNCTC 4 cut(s) 2305, 2337, 5466, 5498
BpmI CTGGAG 9 cut(s) 1712, 1906, 2475, 2748, 3712, 4893, 5957, 6196, 7056
Bpu1102I GCTNAGC 1 cut(s) 2049
BpuEI CTTGAG 7 cut(s) 245, 447, 1663, 3882, 4755, 6168, 6835
BpuMI CCSGG 2 cut(s) 671, 672
Bsa29I ATCGAT 3 cut(s) 1941, 2506, 7172
BsaAI YACGTR 3 cut(s) 16, 235, 6676
BsaBI GATNNNNATC 5 cut(s) 1326, 2024, 2247, 2516, 3679
BsaHI GRCGYC 2 cut(s) 5949, 6874
BsaI GGTCTC 3 cut(s) 1811, 3583, 5638
BsaWI WCCGGW 4 cut(s) 3010, 3326, 4398, 5102
BsaXI ACNNNNNCTCC 4 cut(s) 4536, 4566, 4803, 4833
Bse118I RCCGGY 3 cut(s) 170, 2898, 3326
Bse21I CCTNAGG 3 cut(s) 315, 6429, 7062
Bse3DI GCAATG 5 cut(s) 50, 2867, 5446, 6543, 7260
Bse8I GATNNNNATC 5 cut(s) 1326, 2024, 2247, 2516, 3679
BseAI TCCGGA 1 cut(s) 4398
BseCI ATCGAT 3 cut(s) 1941, 2506, 7172
BseJI GATNNNNATC 5 cut(s) 1326, 2024, 2247, 2516, 3679
BseMI GCAATG 5 cut(s) 50, 2867, 5446, 6543, 7260
BseRI GAGGAG 5 cut(s) 1376, 2555, 3540, 4758, 5373
BseSI GKGCMC 1 cut(s) 5110
BseYI CCCAGC 2 cut(s) 3793, 5242
BsgI GTGCAG 4 cut(s) 2036, 2796, 3318, 4281
Bsh1236I CGCG 1 cut(s) 2617
Bsh1285I CGRYCG 2 cut(s) 136, 799
BshNI GGYRCC 3 cut(s) 3320, 3385, 6611
BshTI ACCGGT 1 cut(s) 3326
BshVI ATCGAT 3 cut(s) 1941, 2506, 7172
BsiEI CGRYCG 2 cut(s) 136, 799
BsiHKAI GWGCWC 7 cut(s) 766, 3120, 3850, 4992, 5092, 5110, 5814
BsiHKCI CYCGRG 2 cut(s) 670, 1456
BsiSI CCGG 8 cut(s) 171, 671, 2899, 3011, 3327, 4399, 5103, 5487
BslFI GGGAC 5 cut(s) 242, 1265, 1672, 2130, 6869
BsmBI CGTCTC 1 cut(s) 5514
BsmFI GGGAC 5 cut(s) 242, 1265, 1672, 2130, 6869
BsmI GAATGC 3 cut(s) 1831, 5893, 6584
Bso31I GGTCTC 3 cut(s) 1811, 3583, 5638
BsoBI CYCGRG 2 cut(s) 670, 1456
Bsp13I TCCGGA 1 cut(s) 4398
Bsp1407I TGTACA 3 cut(s) 490, 4903, 4929
Bsp1720I GCTNAGC 1 cut(s) 2049
Bsp19I CCATGG 4 cut(s) 389, 833, 851, 5314
BspDI ATCGAT 3 cut(s) 1941, 2506, 7172
BspEI TCCGGA 1 cut(s) 4398
BspFNI CGCG 1 cut(s) 2617
BspHI TCATGA 3 cut(s) 5080, 6751, 6958
BspMAI CTGCAG 5 cut(s) 216, 2008, 2014, 2256, 5855
BspMI ACCTGC 2 cut(s) 6617, 6953
BspQI GCTCTTC 1 cut(s) 1635
BspT107I GGYRCC 3 cut(s) 3320, 3385, 6611
BspTI CTTAAG 3 cut(s) 380, 2734, 6532
BspTNI GGTCTC 3 cut(s) 1811, 3583, 5638
BsrDI GCAATG 5 cut(s) 50, 2867, 5446, 6543, 7260
BsrFI RCCGGY 3 cut(s) 170, 2898, 3326
BsrGI TGTACA 3 cut(s) 490, 4903, 4929
BssAI RCCGGY 3 cut(s) 170, 2898, 3326
BssNI GRCGYC 2 cut(s) 5949, 6874
BssSI CACGAG 2 cut(s) 2814, 4724
Bst2BI CACGAG 2 cut(s) 2814, 4724
Bst6I CTCTTC 5 cut(s) 171, 1635, 2532, 5522, 6054
BstACI GRCGYC 2 cut(s) 5949, 6874
BstAFI CTTAAG 3 cut(s) 380, 2734, 6532
BstAPI GCANNNNNTGC 4 cut(s) 326, 1820, 4886, 6257
BstAUI TGTACA 3 cut(s) 490, 4903, 4929
BstBAI YACGTR 3 cut(s) 16, 235, 6676
BstDSI CCRYGG 4 cut(s) 389, 833, 851, 5314
BstEII GGTNACC 3 cut(s) 3583, 3910, 6155
BstFNI CGCG 1 cut(s) 2617
BstH2I RGCGCY 1 cut(s) 2659
BstHHI GCGC 5 cut(s) 2043, 2617, 2658, 4678, 7152
BstMCI CGRYCG 2 cut(s) 136, 799
BstNSI RCATGY 9 cut(s) 842, 1160, 3199, 3574, 4020, 4706, 5834, 7104, 7150
BstPAI GACNNNNGTC 1 cut(s) 2559
BstPI GGTNACC 3 cut(s) 3583, 3910, 6155
BstSFI CTRYAG 5 cut(s) 212, 2004, 2010, 2252, 5851
BstSLI GKGCMC 1 cut(s) 5110
BstUI CGCG 1 cut(s) 2617
BstV2I GAAGAC 8 cut(s) 1551, 2328, 2555, 3458, 3980, 4095, 4764, 6480
BstXI CCANNNNNNTGG 2 cut(s) 876, 7010
Bsu15I ATCGAT 3 cut(s) 1941, 2506, 7172
Bsu36I CCTNAGG 3 cut(s) 315, 6429, 7062
BsuI GTATCC 2 cut(s) 1073, 7194
BsuTUI ATCGAT 3 cut(s) 1941, 2506, 7172
BtgI CCRYGG 4 cut(s) 389, 833, 851, 5314
BtrI CACGTC 1 cut(s) 4537
BtsI GCAGTG 5 cut(s) 2019, 2649, 3355, 5334, 7159
BveI ACCTGC 2 cut(s) 6617, 6953
CaiI CAGNNNCTG 2 cut(s) 273, 2771
CciI TCATGA 3 cut(s) 5080, 6751, 6958
CfoI GCGC 5 cut(s) 2043, 2617, 2658, 4678, 7152
Cfr10I RCCGGY 3 cut(s) 170, 2898, 3326
Cfr9I CCCGGG 1 cut(s) 670
ClaI ATCGAT 3 cut(s) 1941, 2506, 7172
CseI GACGC 4 cut(s) 5957, 6659, 6687, 6863
CspAI ACCGGT 1 cut(s) 3326
CspCI CAANNNNNGTGG 2 cut(s) 5716, 5751
DraI TTTAAA 2 cut(s) 4152, 4411
DrdI GACNNNNNNGTC 1 cut(s) 3575
DseDI GACNNNNNNGTC 1 cut(s) 3575
EaeI YGGCCR 5 cut(s) 403, 1015, 3163, 5715, 6314
Eam1104I CTCTTC 5 cut(s) 171, 1635, 2532, 5522, 6054
EarI CTCTTC 5 cut(s) 171, 1635, 2532, 5522, 6054
EciI GGCGGA 1 cut(s) 74
Eco147I AGGCCT 3 cut(s) 1864, 5392, 6181
Eco24I GRGCYC 5 cut(s) 246, 2432, 5943, 6845, 6936
Eco31I GGTCTC 3 cut(s) 1811, 3583, 5638
Eco32I GATATC 2 cut(s) 1357, 4737
Eco47I GGWCC 7 cut(s) 430, 880, 1252, 2077, 3749, 6629, 7265
Eco72I CACGTG 1 cut(s) 6676
Eco81I CCTNAGG 3 cut(s) 315, 6429, 7062
Eco88I CYCGRG 2 cut(s) 670, 1456
Eco91I GGTNACC 3 cut(s) 3583, 3910, 6155
EcoO109I RGGNCCY 3 cut(s) 256, 7066, 7265
EcoO65I GGTNACC 3 cut(s) 3583, 3910, 6155
EcoRI GAATTC 4 cut(s) 513, 3374, 3641, 5668
EcoRV GATATC 2 cut(s) 1357, 4737
EcoT22I ATGCAT 3 cut(s) 5204, 5585, 7148
EcoT38I GRGCYC 5 cut(s) 246, 2432, 5943, 6845, 6936
Esp3I CGTCTC 1 cut(s) 5514
FaqI GGGAC 5 cut(s) 242, 1265, 1672, 2130, 6869
FauI CCCGC 2 cut(s) 17, 6968
FauNDI CATATG 2 cut(s) 4002, 4452
FbaI TGATCA 1 cut(s) 594
FblI GTMKAC 1 cut(s) 4730
FriOI GRGCYC 5 cut(s) 246, 2432, 5943, 6845, 6936
FspI TGCGCA 2 cut(s) 4677, 7151
GlaI GCGC 5 cut(s) 2042, 2616, 2657, 4677, 7151
GsaI CCCAGC 2 cut(s) 3797, 5246
GsuI CTGGAG 9 cut(s) 1712, 1906, 2475, 2748, 3712, 4893, 5957, 6196, 7056
HaeII RGCGCY 1 cut(s) 2659
HapII CCGG 8 cut(s) 171, 671, 2899, 3011, 3327, 4399, 5103, 5487
HgaI GACGC 4 cut(s) 5957, 6659, 6687, 6863
HhaI GCGC 5 cut(s) 2043, 2617, 2658, 4678, 7152
Hin1I GRCGYC 2 cut(s) 5949, 6874
Hin6I GCGC 5 cut(s) 2041, 2615, 2656, 4676, 7150
HinP1I GCGC 5 cut(s) 2041, 2615, 2656, 4676, 7150
HincII GTYRAC 7 cut(s) 844, 2398, 3403, 4464, 5184, 5346, 6576
HindII GTYRAC 7 cut(s) 844, 2398, 3403, 4464, 5184, 5346, 6576
HindIII AAGCTT 7 cut(s) 398, 1273, 1673, 1739, 1902, 2405, 4373
HpaI GTTAAC 2 cut(s) 844, 4464
HpaII CCGG 8 cut(s) 171, 671, 2899, 3011, 3327, 4399, 5103, 5487
HphI GGTGA 8 cut(s) 293, 1598, 2312, 3577, 4529, 5882, 6167, 6920
Hpy99I CGWCG 4 cut(s) 93, 2279, 2968, 5951
HpyCH4IV ACGT 8 cut(s) 15, 234, 575, 1850, 2277, 2400, 4536, 6675
HpySE526I ACGT 8 cut(s) 15, 234, 575, 1850, 2277, 2400, 4536, 6675
Hsp92I GRCGYC 2 cut(s) 5949, 6874
HspAI GCGC 5 cut(s) 2041, 2615, 2656, 4676, 7150
Kpn2I TCCGGA 1 cut(s) 4398
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 594
KspAI GTTAAC 2 cut(s) 844, 4464
LguI GCTCTTC 1 cut(s) 1635
MaeII ACGT 8 cut(s) 15, 234, 575, 1850, 2277, 2400, 4536, 6675
MfeI CAATTG 1 cut(s) 6821
MlsI TGGCCA 4 cut(s) 405, 1017, 3165, 6316
MluNI TGGCCA 4 cut(s) 405, 1017, 3165, 6316
Mox20I TGGCCA 4 cut(s) 405, 1017, 3165, 6316
Mph1103I ATGCAT 3 cut(s) 5204, 5585, 7148
MroI TCCGGA 1 cut(s) 4398
MroNI GCCGGC 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 5129
MscI TGGCCA 4 cut(s) 405, 1017, 3165, 6316
MslI CAYNNNNRTG 9 cut(s) 874, 1652, 3569, 4337, 5085, 5588, 5997, 6753, 7141
Msp20I TGGCCA 4 cut(s) 405, 1017, 3165, 6316
MspA1I CMGCKG 7 cut(s) 307, 920, 1976, 2009, 2653, 5179, 7249
MspCI CTTAAG 3 cut(s) 380, 2734, 6532
MspI CCGG 8 cut(s) 171, 671, 2899, 3011, 3327, 4399, 5103, 5487
MunI CAATTG 1 cut(s) 6821
Mva1269I GAATGC 3 cut(s) 1831, 5893, 6584
MvnI CGCG 1 cut(s) 2617
NaeI GCCGGC 1 cut(s) 172
NciI CCSGG 2 cut(s) 671, 672
NcoI CCATGG 4 cut(s) 389, 833, 851, 5314
NdeI CATATG 2 cut(s) 4002, 4452
NgoMIV GCCGGC 1 cut(s) 170
NmeAIII GCCGAG 1 cut(s) 1824
NsbI TGCGCA 2 cut(s) 4677, 7151
NsiI ATGCAT 3 cut(s) 5204, 5585, 7148
NspI RCATGY 9 cut(s) 842, 1160, 3199, 3574, 4020, 4706, 5834, 7104, 7150
PaeI GCATGC 3 cut(s) 5834, 7104, 7150
PaeR7I CTCGAG 1 cut(s) 1456
PagI TCATGA 3 cut(s) 5080, 6751, 6958
PceI AGGCCT 3 cut(s) 1864, 5392, 6181
PciI ACATGT 3 cut(s) 1156, 3195, 4702
PciSI GCTCTTC 1 cut(s) 1635
PcsI WCGNNNNNNNCGW 2 cut(s) 2503, 3439
PctI GAATGC 3 cut(s) 1831, 5893, 6584
PdiI GCCGGC 1 cut(s) 172
PdmI GAANNNNTTC 1 cut(s) 5129
PflMI CCANNNNNTGG 3 cut(s) 1076, 4171, 5320
PfoI TCCNGGA 2 cut(s) 4033, 4164
PinAI ACCGGT 1 cut(s) 3326
Ple19I CGATCG 1 cut(s) 799
PmaCI CACGTG 1 cut(s) 6676
PmlI CACGTG 1 cut(s) 6676
Ppu21I YACGTR 3 cut(s) 16, 235, 6676
PpuMI RGGWCCY 1 cut(s) 7265
PscI ACATGT 3 cut(s) 1156, 3195, 4702
PshAI GACNNNNGTC 1 cut(s) 2559
PshBI ATTAAT 2 cut(s) 3860, 4440
PsiI TTATAA 2 cut(s) 1607, 1736
Psp5II RGGWCCY 1 cut(s) 7265
PspCI CACGTG 1 cut(s) 6676
PspEI GGTNACC 3 cut(s) 3583, 3910, 6155
PspFI CCCAGC 2 cut(s) 3793, 5242
PspPPI RGGWCCY 1 cut(s) 7265
PspXI VCTCGAGB 1 cut(s) 1456
PstI CTGCAG 5 cut(s) 216, 2008, 2014, 2256, 5855
PstNI CAGNNNCTG 2 cut(s) 273, 2771
PvuI CGATCG 1 cut(s) 799
PvuII CAGCTG 5 cut(s) 307, 1976, 2009, 5179, 7249
RseI CAYNNNNRTG 9 cut(s) 874, 1652, 3569, 4337, 5085, 5588, 5997, 6753, 7141
SapI GCTCTTC 1 cut(s) 1635
ScaI AGTACT 1 cut(s) 980
SfcI CTRYAG 5 cut(s) 212, 2004, 2010, 2252, 5851
Sfr274I CTCGAG 1 cut(s) 1456
SinI GGWCC 7 cut(s) 430, 880, 1252, 2077, 3749, 6629, 7265
SlaI CTCGAG 1 cut(s) 1456
SmaI CCCGGG 1 cut(s) 672
SmiI ATTTAAAT 1 cut(s) 4152
SmiMI CAYNNNNRTG 9 cut(s) 874, 1652, 3569, 4337, 5085, 5588, 5997, 6753, 7141
SpeI ACTAGT 2 cut(s) 2945, 4274
SphI GCATGC 3 cut(s) 5834, 7104, 7150
SseBI AGGCCT 3 cut(s) 1864, 5392, 6181
SspI AATATT 5 cut(s) 637, 1111, 2856, 4687, 5963
StuI AGGCCT 3 cut(s) 1864, 5392, 6181
SwaI ATTTAAAT 1 cut(s) 4152
TaiI ACGT 8 cut(s) 18, 237, 578, 1853, 2280, 2403, 4539, 6678
TaqII GACCGA 3 cut(s) 122, 1491, 3571
TauI GCSGC 3 cut(s) 2656, 6241, 6796
TspGWI ACGGA 7 cut(s) 669, 3002, 3489, 4540, 5299, 6408, 6555
TspMI CCCGGG 1 cut(s) 670
Van91I CCANNNNNTGG 3 cut(s) 1076, 4171, 5320
Vha464I CTTAAG 3 cut(s) 380, 2734, 6532
VneI GTGCAC 1 cut(s) 5106
VpaK11BI GGWCC 7 cut(s) 430, 880, 1252, 2077, 3749, 6629, 7265
VspI ATTAAT 2 cut(s) 3860, 4440
XbaI TCTAGA 1 cut(s) 766
XceI RCATGY 9 cut(s) 842, 1160, 3199, 3574, 4020, 4706, 5834, 7104, 7150
XcmI CCANNNNNNNNNTGG 1 cut(s) 2092
XhoI CTCGAG 1 cut(s) 1456
XmaI CCCGGG 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 4730
XmnI GAANNNNTTC 1 cut(s) 5129
ZrmI AGTACT 1 cut(s) 980
Zsp2I ATGCAT 3 cut(s) 5204, 5585, 7148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.