pycom06g03700

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004352
Physical Location & Seq
Forward (+)
4670781 .. 4676791
6011 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2478 bp
ATGGAAGTTGGTGATATCAAGGTAGCTAGGTTCACAGACACCACAACTTCTGGGACAAGTTTGGATTTGCAAATTGCTGGAAATTGGAGGCATTCTCAAGTGCAAAACACCATTCAAAATAATAGTGCCTCGCCTGTTGAGCTTATAATTGAGTTTGGAATTGTAGGGATCTCTATTGTTGACCATAGACCAAAAGAAGTCTCCTACTTTTATTTTGAGAGAGTCTTCGTATCATATTGGACCGGTTATGATGGTGGTACAACAACCAGGTTCAAGCTCATACTGGGTCATCTGCAGTTGGACAACCAGCTCCCACTAACAGTGATGCCAGTGCTGTTGGCTCCAGAAATGAACTCTGACATGCATTATCCAGTGTTCAAAACAACTATTACAATGCGCAATGATAATAGTGACGGTGTCCAGGTGTACCCTTATGTTTGTATACGGGTAACTGATAAGAGTTGGAGGCTTAATATTCACGAACCGATAGTTTGGGCTTTGGTGGATTTCTACAATAATCTACAGTTAGATCGTTTGCCTAAAAGTCCTAGTGTCACAGTAGTTGATCCGGAAATACGCATTGACCAAATAGATATTGCAGAAGTTAGGCTGAAGGTGTCATTGGAAACAGCACCAGCCCAAAGACCTCATGGGGTTCTAGGAGTGTGGAGCCCCATACTATCAGCTGTCGGAAGTGCATTCAAGATTCAGGTGCATTTGAGAAGGGTGATGCACAAAGACAGATTCATGCGGAAAAGTTCCATTGTTTCTGCCATTGGTCACCGTATTTGGAGAGATCTGATCCATAATCCTTTACATTTGATATTTTCAGTCGATGTACTTGGTGTGGCAAGTTCAACCTTGGCTTCTCTTAGTAAAGGGTTTGCTGAGCTGTCAACTGATGGACAATTTCAGCAACTGCGTTCGAAGCAGGTGACGTCAAGGAGAATAACTGGGGTGGGTGATGGAATTATGCAAGGTACAGAAGCACTTGTGCAAGGTGTTGCCTTTGGGGTGTCTGGAGTGGTGAATAAGCCTGTAGAGAGTGCAAGACAAGATGGTGTTTTAGGACTAGTTCAAGGGCTTGGGCGTGCTTGTCTAGGAGTGATAGTGCAACCAGTCAGTGGGGCATTGGACTTCTTCTCATTGACTGTTGATGGAATCGGAGCAAGTTGTTCCAAATGTTTAGAGGTTTTCAATAGCAAGACAACCTTCCAGAGAATTAGAAATCCCCGAGCAATCCATGCTGATGCTGTACTTAGAGAATATTGTGAGAGAGAAGCTGTTGGGCAGATGATTTTATACCTTGCAGAAGCACATCGGCACTTTGGTTGTACTGAAATATTCAAGGAGCCATCAAAGTTTGCTTTGTCTGACTATTATGAAGATCATTATGTTGTGCCTTACCAACGGATTGTATTAGTGACTAATAAGCGAGTCATGCTGCTTCAGTGTTTGGCTCCAGATAAAATGGATAAGAAACCTTGCAAGATTATGTGGGATGTACCTTGGGAAGAGCTCATGGCTATGGAGTTGGCAAAAGCAGGCTGCAATCAACCATCTCACTTCATAAATCAAGATCGATCCTCCACCTTAAAAATTTTTTCTCCAAGGAGATCGGAAAATTTTGTTCGGGTGATAAAGTGCATTGTTGAAGAAGTTCCGGGAAATGAACCCCAAGCTGTTAGGATCTGTTCCGTAGTGCGTAAGATGTGGAAAGCATATCAGTCGGACATGAAAAGCCTAATGCTGAAGGTTCCATCAAGCCAGCGACATGTGTATTTTTCCTGGTCTGAAGCTGATGGGAGAGAGCACCCTATACAGAACAAGGCAATTACTAGGCTGAGAGAGCTCCCATCATACAATTCTGCATTGAATGATAGATTTGTTAAACACAACATCAACTTTTCTAAGATTTGGAGCAGCGAACAAGAATCCAGAAGTCGATGCATGTTGTGCAGAAAACAGGTTCCCGAAGATGATGTAATATGTAGCATTTGGAGACCCATCTGTCCTGACGGCTATGTCTCGATTGGTGATATTGCTCACATTGGCAGCCATCCTCCAAACGTTGCTGCTGTTTATTGCAAGATTGACAGATTGTTTGCACTTCCAGTTGGTTATGACTTGGTATGGAGGAACTGCCTGGAGGACTATGCAGCACCGGTATCAATTTGGCATCCTCGGGCTCCAGAGGGATATGTGTCTCCCGGATGCATTGCTGTGCCTAGTTTTATTGAGCCAGAACTTGATATAGCGTATTGTGTGGCTGAATCATTTGCTGAAGAGGCGGAATTCGAAGGGCAAAGGGTTTGGTCTGCTCCAGATTCTTACCCCTGGGCGTGTCACATTTATCAAGTCCAAAGCGACGCACTTCACCTTGTGGCTCTAAGGCAAACCAAGGAAGAATCTGGCTGGAAACCTATGAGGGTTATTAACGATCCTCGGCCTCTATTGGAATCACTAGAAGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N PH Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

826

Amino Acids

93.15

Weight (kDa)

6.79

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_C PF25037 408 - 516 1.9e-14 Intermembrane lipid transfer protein VPS13, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 146
AarI CACCTGC 1 cut(s) 922
AasI GACNNNNNNGTC 2 cut(s) 2010, 2024
AatII GACGTC 1 cut(s) 941
Acc16I TGCGCA 1 cut(s) 398
Acc36I ACCTGC 1 cut(s) 922
AccB7I CCANNNNNTGG 1 cut(s) 1124
AccI GTMKAC 1 cut(s) 442
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 2 cut(s) 751, 2291
AclI AACGTT 1 cut(s) 2070
AclWI GGATC 6 cut(s) 176, 560, 796, 1578, 1697, 2435
AcsI RAATTY 3 cut(s) 1599, 1624, 2294
AcuI CTGAAG 5 cut(s) 632, 1433, 1772, 1815, 2304
AcyI GRCGYC 1 cut(s) 938
AdeI CACNNNGTG 1 cut(s) 2383
AfaI GTAC 7 cut(s) 259, 428, 840, 982, 1257, 1336, 1506
AfiI CCNNNNNNNGG 1 cut(s) 1124
AflIII ACRYGT 1 cut(s) 1774
AgeI ACCGGT 2 cut(s) 242, 2164
AhlI ACTAGT 1 cut(s) 1072
AjnI CCWGG 5 cut(s) 266, 420, 1787, 2145, 2336
AjuI GAANNNNNNNTTGG 2 cut(s) 605, 637
Alw21I GWGCWC 3 cut(s) 1521, 1815, 1854
Alw26I GTCTC 4 cut(s) 205, 1996, 2032, 2211
AlwI GGATC 6 cut(s) 176, 560, 796, 1578, 1697, 2435
AlwNI CAGNNNCTG 1 cut(s) 919
Ama87I CYCGRG 2 cut(s) 1233, 2184
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 1 cut(s) 2447
ApeKI GCWGC 6 cut(s) 1444, 1548, 1923, 2055, 2075, 2159
ApoI RAATTY 3 cut(s) 1599, 1624, 2294
ArsI GACNNNNNNTTYG 2 cut(s) 2087, 2119
AsiGI ACCGGT 2 cut(s) 242, 2164
Asp700I GAANNNNTTC 1 cut(s) 1659
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 1 cut(s) 240
AsuC2I CCSGG 2 cut(s) 1665, 2211
AsuHPI GGTGA 9 cut(s) 23, 739, 773, 946, 974, 1039, 1648, 2048, 2369
AsuII TTCGAA 2 cut(s) 926, 2298
AvaI CYCGRG 2 cut(s) 1233, 2184
AvaII GGWCC 1 cut(s) 240
BanII GRGCYC 4 cut(s) 674, 1521, 1854, 2191
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 3 cut(s) 1521, 1815, 1854
BbvI GCAGC 6 cut(s) 1431, 1535, 1935, 2062, 2067, 2171
BceAI ACGGC 1 cut(s) 2035
BcgI CGANNNNNNTGC 2 cut(s) 1710, 1744
BciT130I CCWGG 5 cut(s) 268, 422, 1789, 2147, 2338
BcnI CCSGG 2 cut(s) 1665, 2211
BcoDI GTCTC 4 cut(s) 205, 1996, 2032, 2211
BcuI ACTAGT 1 cut(s) 1072
BfaI CTAG 8 cut(s) 27, 549, 659, 1073, 1100, 1839, 2229, 2465
BfmI CTRYAG 3 cut(s) 293, 521, 1038
BfuAI ACCTGC 1 cut(s) 922
BglII AGATCT 1 cut(s) 796
BisI GCNGC 6 cut(s) 1445, 1549, 1924, 2056, 2076, 2160
BlpI GCTNAGC 1 cut(s) 888
BlsI GCNGC 6 cut(s) 1446, 1550, 1925, 2057, 2077, 2161
Bme1390I CCNGG 7 cut(s) 268, 422, 1665, 1789, 2147, 2211, 2338
Bme18I GGWCC 1 cut(s) 240
BmeT110I CYCGRG 2 cut(s) 1233, 2184
BmgT120I GGNCC 1 cut(s) 240
BmiI GGNNCC 7 cut(s) 342, 671, 1353, 1461, 1758, 1971, 2190
BmrFI CCNGG 7 cut(s) 268, 422, 1665, 1789, 2147, 2211, 2338
BmrI ACTGGG 2 cut(s) 293, 963
BmsI GCATC 6 cut(s) 315, 720, 1240, 1937, 2188, 2204
BmuI ACTGGG 2 cut(s) 293, 963
BpiI GAAGAC 1 cut(s) 217
BplI GAGNNNNNCTC 2 cut(s) 79, 111
BpmI CTGGAG 6 cut(s) 327, 1041, 1446, 2168, 2175, 2307
Bpu1102I GCTNAGC 1 cut(s) 888
Bpu14I TTCGAA 2 cut(s) 926, 2298
BpuEI CTTGAG 1 cut(s) 81
BpuMI CCSGG 2 cut(s) 1665, 2211
Bsa29I ATCGAT 1 cut(s) 1582
BsaHI GRCGYC 1 cut(s) 938
BsaI GGTCTC 1 cut(s) 1996
BsaJI CCNNGG 8 cut(s) 861, 1508, 1610, 2183, 2336, 2337, 2400, 2444
BsaWI WCCGGW 3 cut(s) 242, 568, 2164
BsaXI ACNNNNNCTCC 2 cut(s) 1095, 1125
Bsc4I CCNNNNNNNGG 1 cut(s) 1124
Bse118I RCCGGY 2 cut(s) 242, 2164
Bse1I ACTGG 6 cut(s) 288, 329, 371, 958, 1118, 2114
Bse3DI GCAATG 2 cut(s) 406, 2217
BseAI TCCGGA 1 cut(s) 568
BseBI CCWGG 5 cut(s) 268, 422, 1789, 2147, 2338
BseCI ATCGAT 1 cut(s) 1582
BseDI CCNNGG 8 cut(s) 861, 1508, 1610, 2183, 2336, 2337, 2400, 2444
BseGI GGATG 4 cut(s) 1507, 2059, 2179, 2219
BseLI CCNNNNNNNGG 1 cut(s) 1124
BseMI GCAATG 2 cut(s) 406, 2217
BseMII CTCAG 2 cut(s) 879, 1835
BseNI ACTGG 6 cut(s) 288, 329, 371, 958, 1118, 2114
BseXI GCAGC 6 cut(s) 1431, 1535, 1935, 2062, 2067, 2171
BsgI GTGCAG 1 cut(s) 1978
BshFI GGCC 1 cut(s) 2449
BshTI ACCGGT 2 cut(s) 242, 2164
BshVI ATCGAT 1 cut(s) 1582
BsiHKAI GWGCWC 3 cut(s) 1521, 1815, 1854
BsiHKCI CYCGRG 2 cut(s) 1233, 2184
BsiSI CCGG 5 cut(s) 243, 569, 1664, 2165, 2211
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 1124
BsmAI GTCTC 4 cut(s) 205, 1996, 2032, 2211
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 2 cut(s) 91, 698
BsnI GGCC 1 cut(s) 2449
Bso31I GGTCTC 1 cut(s) 1996
BsoBI CYCGRG 2 cut(s) 1233, 2184
Bsp119I TTCGAA 2 cut(s) 926, 2298
Bsp1286I GDGCHC 5 cut(s) 674, 1521, 1815, 1854, 2191
Bsp13I TCCGGA 1 cut(s) 568
Bsp1720I GCTNAGC 1 cut(s) 888
BspACI CCGC 2 cut(s) 751, 2291
BspANI GGCC 1 cut(s) 2449
BspCNI CTCAG 2 cut(s) 880, 1836
BspDI ATCGAT 1 cut(s) 1582
BspEI TCCGGA 1 cut(s) 568
BspLI GGNNCC 7 cut(s) 342, 671, 1353, 1461, 1758, 1971, 2190
BspMAI CTGCAG 1 cut(s) 297
BspMI ACCTGC 1 cut(s) 922
BspPI GGATC 6 cut(s) 176, 560, 796, 1578, 1697, 2435
BspQI GCTCTTC 1 cut(s) 1509
BspT104I TTCGAA 2 cut(s) 926, 2298
BspTNI GGTCTC 1 cut(s) 1996
BsrDI GCAATG 2 cut(s) 406, 2217
BsrFI RCCGGY 2 cut(s) 242, 2164
BsrI ACTGG 6 cut(s) 288, 329, 371, 958, 1118, 2114
BssAI RCCGGY 2 cut(s) 242, 2164
BssECI CCNNGG 8 cut(s) 861, 1508, 1610, 2183, 2336, 2337, 2400, 2444
BssNAI GTATAC 1 cut(s) 443
BssNI GRCGYC 1 cut(s) 938
BssT1I CCWWGG 4 cut(s) 861, 1508, 1610, 2400
Bst1107I GTATAC 1 cut(s) 443
Bst2UI CCWGG 5 cut(s) 268, 422, 1789, 2147, 2338
Bst4CI ACNGT 6 cut(s) 322, 416, 525, 559, 785, 1153
Bst6I CTCTTC 3 cut(s) 1509, 2280, 2463
BstACI GRCGYC 1 cut(s) 938
BstAPI GCANNNNNTGC 2 cut(s) 1244, 1956
BstBI TTCGAA 2 cut(s) 926, 2298
BstC8I GCNNGC 3 cut(s) 1092, 1546, 1769
BstDEI CTNAG 6 cut(s) 872, 888, 1259, 1844, 1911, 2390
BstEII GGTNACC 1 cut(s) 779
BstF5I GGATG 4 cut(s) 1507, 2059, 2179, 2219
BstHHI GCGC 1 cut(s) 399
BstMAI GTCTC 4 cut(s) 205, 1996, 2032, 2211
BstMWI GCNNNNNNNGC 7 cut(s) 139, 928, 1244, 1441, 1849, 1956, 2288
BstNI CCWGG 5 cut(s) 268, 422, 1789, 2147, 2338
BstNSI RCATGY 3 cut(s) 364, 1778, 1954
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 7 cut(s) 266, 420, 1663, 1787, 2145, 2209, 2336
BstSFI CTRYAG 3 cut(s) 293, 521, 1038
BstV1I GCAGC 6 cut(s) 1431, 1535, 1935, 2062, 2067, 2171
BstV2I GAAGAC 1 cut(s) 217
BstX2I RGATCY 3 cut(s) 168, 796, 1689
BstYI RGATCY 3 cut(s) 168, 796, 1689
BstZ17I GTATAC 1 cut(s) 443
Bsu15I ATCGAT 1 cut(s) 1582
BsuRI GGCC 1 cut(s) 2449
BsuTUI ATCGAT 1 cut(s) 1582
BtsCI GGATG 4 cut(s) 1507, 2059, 2179, 2219
BtsIMutI CAGTG 5 cut(s) 327, 336, 378, 1129, 1457
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 3 cut(s) 1092, 1546, 1769
CaiI CAGNNNCTG 1 cut(s) 919
CfoI GCGC 1 cut(s) 399
Cfr10I RCCGGY 2 cut(s) 242, 2164
Cfr13I GGNCC 1 cut(s) 240
ClaI ATCGAT 1 cut(s) 1582
CseI GACGC 1 cut(s) 2378
CsiI ACCWGGT 1 cut(s) 266
Csp6I GTAC 7 cut(s) 258, 427, 839, 981, 1256, 1335, 1505
CspAI ACCGGT 2 cut(s) 242, 2164
CviAII CATG 9 cut(s) 361, 650, 748, 1244, 1441, 1522, 1735, 1775, 1951
CviQI GTAC 7 cut(s) 258, 427, 839, 981, 1256, 1335, 1505
DdeI CTNAG 6 cut(s) 872, 888, 1259, 1844, 1911, 2390
DraIII CACNNNGTG 1 cut(s) 2383
DrdI GACNNNNNNGTC 2 cut(s) 2010, 2024
DseDI GACNNNNNNGTC 2 cut(s) 2010, 2024
Eam1104I CTCTTC 3 cut(s) 1509, 2280, 2463
EarI CTCTTC 3 cut(s) 1509, 2280, 2463
EciI GGCGGA 1 cut(s) 2306
Ecl136II GAGCTC 2 cut(s) 1519, 1852
Eco130I CCWWGG 4 cut(s) 861, 1508, 1610, 2400
Eco24I GRGCYC 4 cut(s) 674, 1521, 1854, 2191
Eco31I GGTCTC 1 cut(s) 1996
Eco32I GATATC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 240
Eco53kI GAGCTC 2 cut(s) 1519, 1852
Eco57I CTGAAG 5 cut(s) 632, 1433, 1772, 1815, 2304
Eco88I CYCGRG 2 cut(s) 1233, 2184
Eco91I GGTNACC 1 cut(s) 779
EcoICRI GAGCTC 2 cut(s) 1519, 1852
EcoO65I GGTNACC 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 2294
EcoRII CCWGG 5 cut(s) 266, 420, 1787, 2145, 2336
EcoRV GATATC 1 cut(s) 16
EcoT14I CCWWGG 4 cut(s) 861, 1508, 1610, 2400
EcoT22I ATGCAT 3 cut(s) 366, 1952, 2219
EcoT38I GRGCYC 4 cut(s) 674, 1521, 1854, 2191
ErhI CCWWGG 4 cut(s) 861, 1508, 1610, 2400
FaeI CATG 9 cut(s) 364, 653, 751, 1247, 1444, 1525, 1738, 1778, 1954
FalI AAGNNNNNCTT 2 cut(s) 1196, 1228
FaqI GGGAC 1 cut(s) 67
FatI CATG 9 cut(s) 360, 649, 747, 1243, 1440, 1521, 1734, 1774, 1950
FblI GTMKAC 1 cut(s) 442
Fnu4HI GCNGC 6 cut(s) 1445, 1549, 1924, 2056, 2076, 2160
FokI GGATG 4 cut(s) 1514, 2046, 2166, 2226
FriOI GRGCYC 4 cut(s) 674, 1521, 1854, 2191
Fsp4HI GCNGC 6 cut(s) 1445, 1549, 1924, 2056, 2076, 2160
FspBI CTAG 8 cut(s) 27, 549, 659, 1073, 1100, 1839, 2229, 2465
FspI TGCGCA 1 cut(s) 398
GlaI GCGC 1 cut(s) 398
GluI GCNGC 6 cut(s) 1445, 1549, 1924, 2056, 2076, 2160
GsuI CTGGAG 6 cut(s) 327, 1041, 1446, 2168, 2175, 2307
HaeIII GGCC 1 cut(s) 2449
HapII CCGG 5 cut(s) 243, 569, 1664, 2165, 2211
HgaI GACGC 1 cut(s) 2378
HhaI GCGC 1 cut(s) 399
Hin1I GRCGYC 1 cut(s) 938
Hin1II CATG 9 cut(s) 364, 653, 751, 1247, 1444, 1525, 1738, 1778, 1954
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HincII GTYRAC 2 cut(s) 181, 897
HindII GTYRAC 2 cut(s) 181, 897
HpaII CCGG 5 cut(s) 243, 569, 1664, 2165, 2211
HphI GGTGA 9 cut(s) 23, 739, 773, 946, 974, 1039, 1648, 2048, 2369
Hpy166II GTNNAC 5 cut(s) 33, 181, 427, 443, 897
Hpy188I TCNGA 8 cut(s) 358, 692, 801, 1166, 1375, 1621, 1732, 1795
Hpy8I GTNNAC 5 cut(s) 33, 181, 427, 443, 897
Hpy99I CGWCG 1 cut(s) 2372
HpyAV CCTTC 5 cut(s) 607, 717, 1222, 1747, 2294
HpyCH4III ACNGT 6 cut(s) 322, 416, 525, 559, 785, 1153
HpyCH4IV ACGT 2 cut(s) 938, 2070
HpyF10VI GCNNNNNNNGC 7 cut(s) 139, 928, 1244, 1441, 1849, 1956, 2288
HpyF3I CTNAG 6 cut(s) 872, 888, 1259, 1844, 1911, 2390
HpySE526I ACGT 2 cut(s) 938, 2070
Hsp92I GRCGYC 1 cut(s) 938
Hsp92II CATG 9 cut(s) 364, 653, 751, 1247, 1444, 1525, 1738, 1778, 1954
HspAI GCGC 1 cut(s) 397
Kpn2I TCCGGA 1 cut(s) 568
LguI GCTCTTC 1 cut(s) 1509
Lsp1109I GCAGC 6 cut(s) 1431, 1535, 1935, 2062, 2067, 2171
LweI GCATC 6 cut(s) 315, 720, 1240, 1937, 2188, 2204
MabI ACCWGGT 1 cut(s) 266
MaeI CTAG 8 cut(s) 27, 549, 659, 1073, 1100, 1839, 2229, 2465
MaeII ACGT 2 cut(s) 938, 2070
MaeIII GTNAC 7 cut(s) 410, 448, 553, 779, 934, 1423, 2345
MboII GAAGA 8 cut(s) 217, 1132, 1397, 1526, 1667, 1988, 2297, 2417
MflI RGATCY 3 cut(s) 168, 796, 1689
MhlI GDGCHC 5 cut(s) 674, 1521, 1815, 1854, 2191
MlyI GAGTC 2 cut(s) 231, 1446
MmeI TCCRAC 4 cut(s) 279, 443, 670, 1710
Mph1103I ATGCAT 3 cut(s) 366, 1952, 2219
MroI TCCGGA 1 cut(s) 568
MroXI GAANNNNTTC 1 cut(s) 1659
MseI TTAA 4 cut(s) 471, 1595, 1890, 2436
MslI CAYNNNNRTG 3 cut(s) 1248, 1526, 2222
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 5 cut(s) 243, 569, 1664, 2165, 2211
MspR9I CCNGG 7 cut(s) 268, 422, 1665, 1789, 2147, 2211, 2338
Mva1269I GAATGC 2 cut(s) 91, 698
MvaI CCWGG 5 cut(s) 268, 422, 1789, 2147, 2338
MwoI GCNNNNNNNGC 7 cut(s) 139, 928, 1244, 1441, 1849, 1956, 2288
NciI CCSGG 2 cut(s) 1665, 2211
NlaIII CATG 9 cut(s) 364, 653, 751, 1247, 1444, 1525, 1738, 1778, 1954
NlaIV GGNNCC 7 cut(s) 342, 671, 1353, 1461, 1758, 1971, 2190
NmeAIII GCCGAG 1 cut(s) 2425
NmuCI GTSAC 6 cut(s) 410, 553, 779, 934, 1423, 2345
NsbI TGCGCA 1 cut(s) 398
NsiI ATGCAT 3 cut(s) 366, 1952, 2219
NspI RCATGY 3 cut(s) 364, 1778, 1954
NspV TTCGAA 2 cut(s) 926, 2298
PaqCI CACCTGC 1 cut(s) 922
PasI CCCWGGG 1 cut(s) 2337
PciI ACATGT 1 cut(s) 1774
PciSI GCTCTTC 1 cut(s) 1509
PctI GAATGC 2 cut(s) 91, 698
PdmI GAANNNNTTC 1 cut(s) 1659
PfeI GAWTC 8 cut(s) 706, 744, 1161, 1934, 2273, 2327, 2408, 2459
PflFI GACNNNGTC 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 1124
PfoI TCCNGGA 2 cut(s) 1663, 2209
PinAI ACCGGT 2 cut(s) 242, 2164
PkrI GCNGC 6 cut(s) 1446, 1550, 1925, 2057, 2077, 2161
PleI GAGTC 2 cut(s) 230, 1445
PpsI GAGTC 2 cut(s) 230, 1445
PscI ACATGT 1 cut(s) 1774
PsiI TTATAA 1 cut(s) 146
Psp124BI GAGCTC 2 cut(s) 1521, 1854
Psp1406I AACGTT 1 cut(s) 2070
Psp6I CCWGG 5 cut(s) 266, 420, 1787, 2145, 2336
PspEI GGTNACC 1 cut(s) 779
PspGI CCWGG 5 cut(s) 266, 420, 1787, 2145, 2336
PspN4I GGNNCC 7 cut(s) 342, 671, 1353, 1461, 1758, 1971, 2190
PspPI GGNCC 1 cut(s) 240
PstI CTGCAG 1 cut(s) 297
PstNI CAGNNNCTG 1 cut(s) 919
PsuI RGATCY 3 cut(s) 168, 796, 1689
PsyI GACNNNGTC 1 cut(s) 416
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 7 cut(s) 259, 428, 840, 982, 1257, 1336, 1506
RsaNI GTAC 7 cut(s) 258, 427, 839, 981, 1256, 1335, 1505
RseI CAYNNNNRTG 3 cut(s) 1248, 1526, 2222
SacI GAGCTC 2 cut(s) 1521, 1854
SapI GCTCTTC 1 cut(s) 1509
SaqAI TTAA 4 cut(s) 471, 1595, 1890, 2436
SatI GCNGC 6 cut(s) 1445, 1549, 1924, 2056, 2076, 2160
Sau96I GGNCC 1 cut(s) 240
SchI GAGTC 2 cut(s) 231, 1446
ScrFI CCNGG 7 cut(s) 268, 422, 1665, 1789, 2147, 2211, 2338
SduI GDGCHC 5 cut(s) 674, 1521, 1815, 1854, 2191
SexAI ACCWGGT 1 cut(s) 266
SfaNI GCATC 6 cut(s) 315, 720, 1240, 1937, 2188, 2204
SfcI CTRYAG 3 cut(s) 293, 521, 1038
SfuI TTCGAA 2 cut(s) 926, 2298
SinI GGWCC 1 cut(s) 240
SmiMI CAYNNNNRTG 3 cut(s) 1248, 1526, 2222
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
SpeI ACTAGT 1 cut(s) 1072
SsiI CCGC 2 cut(s) 751, 2291
SspI AATATT 3 cut(s) 475, 1268, 1344
SspMI CTAG 8 cut(s) 27, 549, 659, 1073, 1100, 1839, 2229, 2465
SstI GAGCTC 2 cut(s) 1521, 1854
StyD4I CCNGG 7 cut(s) 266, 420, 1663, 1787, 2145, 2209, 2336
StyI CCWWGG 4 cut(s) 861, 1508, 1610, 2400
TaaI ACNGT 6 cut(s) 322, 416, 525, 559, 785, 1153
TaiI ACGT 2 cut(s) 941, 2073
TaqI TCGA 6 cut(s) 834, 926, 1582, 1945, 2030, 2298
TatI WGTACW 3 cut(s) 838, 1255, 1334
TfiI GAWTC 8 cut(s) 706, 744, 1161, 1934, 2273, 2327, 2408, 2459
Tru1I TTAA 4 cut(s) 471, 1595, 1890, 2436
Tru9I TTAA 4 cut(s) 471, 1595, 1890, 2436
TscAI CASTG 5 cut(s) 327, 336, 378, 1129, 1457
TseFI GTSAC 6 cut(s) 410, 553, 779, 934, 1423, 2345
TseI GCWGC 6 cut(s) 1444, 1548, 1923, 2055, 2075, 2159
Tsp45I GTSAC 6 cut(s) 410, 553, 779, 934, 1423, 2345
TspDTI ATGAA 6 cut(s) 365, 736, 1398, 1558, 1686, 1751
TspGWI ACGGA 2 cut(s) 1426, 1687
TspRI CASTG 5 cut(s) 327, 336, 378, 1129, 1457
Tth111I GACNNNGTC 1 cut(s) 416
Van91I CCANNNNNTGG 1 cut(s) 1124
VpaK11BI GGWCC 1 cut(s) 240
XapI RAATTY 3 cut(s) 1599, 1624, 2294
XceI RCATGY 3 cut(s) 364, 1778, 1954
XcmI CCANNNNNNNNNTGG 1 cut(s) 647
XmiI GTMKAC 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 1659
XspI CTAG 8 cut(s) 27, 549, 659, 1073, 1100, 1839, 2229, 2465
ZraI GACGTC 1 cut(s) 939
Zsp2I ATGCAT 3 cut(s) 366, 1952, 2219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.