pycom06g06400

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010311
Physical Location & Seq
Forward (+)
10069233 .. 10072348
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1029 bp
ATGACAATAGCATTAGATGACTTGAATGGGTTGGAATTATTGTTTTTATTGTCGTCTTGTAAATCTGTTGCCGGTTGTAGCGGATCGGGTCTCTTCAGTCTATTCCCTTCGTCTTCCTCCTATTCTATCTCACTCGCTCGCTCTCTGGTGGTGAAAATGGCAGTCTCTTTTCACCCAACGACTACTTCTCCAGGCCTTCATACCCAAGCTAGACTCTCCCTTCCTCCTCCCAGTTGTGCAACGAAGCATTATTCAGGATTAAAGCTTCAGTCTTTAGGTACTTTTGGTGCTAAAAACCCTAACTTGACGGTTGAGTTCTACGGAAAAGTTAATGAGAGCCTTCAACCCAGGACACGAAACCATAGGCCTTCACGAGCACAAGTTGGAATGATGCCTATAGGGACACCAAAAGTTCCCTACAGAACTCCTGGTGAGGGAACTTGGCAATGGGTTGATTTGTGGAATGCTCTATACAGAGAACGTGTGATCTTCATCGGGCAAAACATAGATGAAGAGTTCAGCAATCAAATTTTGGCAACAATGTTGTACCTTGACACAACAGATTCTTCGAAAAGGCTTTATATGTACATCAATGGTCCTGGTGGAGATCTTACTCCAAGCATGGCTATCTATGATACGATGCAGAGCCTAAAAAGTCCTGTTGGTACCCATTGTGTGGGCTATGCCTATAATTTAGCAGCCTTTCTTCTTGCCGCTGGAGAGAAGGGAAATCGTTTTGCAATGCCACTATCAAGAATTGCTCTACAGTCTCCTGCCGGGGCTGCTCGTGGTCAGGCTGATGATATACAAAATGAAGCAAATGAACTTCTCAGAATTAGAGATTACCTGTTTAATGAGTTGGCTATGAAAACAGGCCAGCCAGTTGAAAAGATTAACAAAGACTTAAGTAGGATGAAGCGCTTTAATGCGCAGGAGGCTCTTGAATATGGGCTCATTGATCGTGTTGTTAGGCCACCCCGTATCAAGGCTGATGCACCTCCCAAGGACAGCGGATCAGGTCTTGGTTAG

Protein Analysis

343

Amino Acids

37.44

Weight (kDa)

9.16

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 149 - 324 3.3e-64 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 930
Acc65I GGTACC 1 cut(s) 665
AccB1I GGYRCC 1 cut(s) 665
AccB7I CCANNNNNTGG 1 cut(s) 676
AciI CCGC 3 cut(s) 81, 714, 1011
AclWI GGATC 2 cut(s) 91, 1021
AcsI RAATTY 1 cut(s) 528
AcuI CTGAAG 2 cut(s) 79, 251
AfaI GTAC 4 cut(s) 280, 548, 587, 667
AfeI AGCGCT 1 cut(s) 920
AfiI CCNNNNNNNGG 3 cut(s) 434, 676, 985
AflII CTTAAG 1 cut(s) 904
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 4 cut(s) 25, 344, 887, 944
AjnI CCWGG 4 cut(s) 190, 347, 427, 598
AjuI GAANNNNNNNTTGG 2 cut(s) 169, 201
AluBI AGCT 2 cut(s) 209, 265
AluI AGCT 2 cut(s) 209, 265
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 3 cut(s) 95, 169, 774
AlwI GGATC 2 cut(s) 91, 1021
Aor51HI AGCGCT 1 cut(s) 920
AoxI GGCC 4 cut(s) 193, 365, 874, 971
ApeKI GCWGC 2 cut(s) 698, 782
ApoI RAATTY 1 cut(s) 528
Asp718I GGTACC 1 cut(s) 665
AspLEI GCGC 2 cut(s) 921, 931
AspS9I GGNCC 1 cut(s) 596
AsuC2I CCSGG 1 cut(s) 778
AsuHPI GGTGA 3 cut(s) 163, 164, 443
AsuII TTCGAA 1 cut(s) 569
AvaII GGWCC 1 cut(s) 596
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 954
BauI CACGAG 2 cut(s) 372, 786
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 379
BbvI GCAGC 2 cut(s) 710, 769
BciT130I CCWGG 4 cut(s) 192, 349, 429, 600
BcnI CCSGG 1 cut(s) 778
BcoDI GTCTC 3 cut(s) 95, 169, 774
BfaI CTAG 1 cut(s) 210
BfmI CTRYAG 3 cut(s) 396, 418, 764
BfoI RGCGCY 1 cut(s) 922
BfrI CTTAAG 1 cut(s) 904
BglII AGATCT 1 cut(s) 607
BisI GCNGC 3 cut(s) 699, 714, 783
BlsI GCNGC 3 cut(s) 700, 715, 784
Bme1390I CCNGG 5 cut(s) 192, 349, 429, 600, 778
Bme18I GGWCC 1 cut(s) 596
BmgT120I GGNCC 1 cut(s) 596
BmiI GGNNCC 1 cut(s) 667
BmrFI CCNGG 5 cut(s) 192, 349, 429, 600, 778
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 3 cut(s) 381, 630, 982
BmuI ACTGGG 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 2 cut(s) 174, 738
Bpu14I TTCGAA 1 cut(s) 569
BpuMI CCSGG 1 cut(s) 778
BsaBI GATNNNNATC 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 347, 777, 1002
BsaXI ACNNNNNCTCC 2 cut(s) 172, 202
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 676, 985
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 231, 881
Bse3DI GCAATG 2 cut(s) 452, 747
Bse8I GATNNNNATC 1 cut(s) 491
BseBI CCWGG 4 cut(s) 192, 349, 429, 600
BseDI CCNNGG 3 cut(s) 347, 777, 1002
BseGI GGATG 1 cut(s) 918
BseJI GATNNNNATC 1 cut(s) 491
BseLI CCNNNNNNNGG 3 cut(s) 434, 676, 985
BseMI GCAATG 2 cut(s) 452, 747
BseMII CTCAG 1 cut(s) 844
BseNI ACTGG 2 cut(s) 231, 881
BseRI GAGGAG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 710, 769
BshFI GGCC 4 cut(s) 195, 367, 876, 973
BshNI GGYRCC 1 cut(s) 665
BsiHKAI GWGCWC 1 cut(s) 379
BsiSI CCGG 2 cut(s) 72, 777
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 3 cut(s) 434, 676, 985
BsmAI GTCTC 3 cut(s) 95, 169, 774
BsmFI GGGAC 1 cut(s) 415
BsmI GAATGC 1 cut(s) 469
BsnI GGCC 4 cut(s) 195, 367, 876, 973
Bso31I GGTCTC 1 cut(s) 95
Bsp119I TTCGAA 1 cut(s) 569
Bsp1286I GDGCHC 2 cut(s) 379, 954
Bsp1407I TGTACA 1 cut(s) 585
Bsp143I GATC 5 cut(s) 83, 486, 607, 958, 1013
BspACI CCGC 3 cut(s) 81, 714, 1011
BspANI GGCC 4 cut(s) 195, 367, 876, 973
BspCNI CTCAG 1 cut(s) 843
BspLI GGNNCC 1 cut(s) 667
BspPI GGATC 2 cut(s) 91, 1021
BspT104I TTCGAA 1 cut(s) 569
BspT107I GGYRCC 1 cut(s) 665
BspTI CTTAAG 1 cut(s) 904
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 2 cut(s) 452, 747
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 585
BsrI ACTGG 2 cut(s) 231, 881
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 3 cut(s) 347, 777, 1002
BssMI GATC 5 cut(s) 83, 486, 607, 958, 1013
BssSI CACGAG 2 cut(s) 372, 786
BssT1I CCWWGG 1 cut(s) 1002
Bst2BI CACGAG 2 cut(s) 372, 786
Bst2UI CCWGG 4 cut(s) 192, 349, 429, 600
Bst4CI ACNGT 2 cut(s) 310, 768
Bst6I CTCTTC 2 cut(s) 98, 507
BstAFI CTTAAG 1 cut(s) 904
BstAUI TGTACA 1 cut(s) 585
BstBI TTCGAA 1 cut(s) 569
BstC8I GCNNGC 2 cut(s) 139, 878
BstDEI CTNAG 1 cut(s) 830
BstF5I GGATG 1 cut(s) 918
BstH2I RGCGCY 1 cut(s) 922
BstHHI GCGC 2 cut(s) 921, 931
BstKTI GATC 5 cut(s) 86, 489, 610, 961, 1016
BstMAI GTCTC 3 cut(s) 95, 169, 774
BstMBI GATC 5 cut(s) 83, 486, 607, 958, 1013
BstMWI GCNNNNNNNGC 2 cut(s) 782, 935
BstNI CCWGG 4 cut(s) 192, 349, 429, 600
BstSCI CCNGG 5 cut(s) 190, 347, 427, 598, 776
BstSFI CTRYAG 3 cut(s) 396, 418, 764
BstV1I GCAGC 2 cut(s) 710, 769
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
BsuRI GGCC 4 cut(s) 195, 367, 876, 973
BtsCI GGATG 1 cut(s) 918
Cac8I GCNNGC 2 cut(s) 139, 878
CfoI GCGC 2 cut(s) 921, 931
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 596
Csp6I GTAC 4 cut(s) 279, 547, 586, 666
CviAII CATG 1 cut(s) 622
CviQI GTAC 4 cut(s) 279, 547, 586, 666
DdeI CTNAG 1 cut(s) 830
DpnI GATC 5 cut(s) 85, 488, 609, 960, 1015
DpnII GATC 5 cut(s) 83, 486, 607, 958, 1013
Eam1104I CTCTTC 2 cut(s) 98, 507
EarI CTCTTC 2 cut(s) 98, 507
Eco130I CCWWGG 1 cut(s) 1002
Eco147I AGGCCT 2 cut(s) 195, 367
Eco24I GRGCYC 1 cut(s) 954
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 596
Eco47III AGCGCT 1 cut(s) 920
Eco57I CTGAAG 2 cut(s) 79, 251
EcoRII CCWGG 4 cut(s) 190, 347, 427, 598
EcoT14I CCWWGG 1 cut(s) 1002
EcoT38I GRGCYC 1 cut(s) 954
ErhI CCWWGG 1 cut(s) 1002
FaeI CATG 1 cut(s) 625
FaqI GGGAC 1 cut(s) 415
FatI CATG 1 cut(s) 621
Fnu4HI GCNGC 3 cut(s) 699, 714, 783
FokI GGATG 1 cut(s) 925
FriOI GRGCYC 1 cut(s) 954
Fsp4HI GCNGC 3 cut(s) 699, 714, 783
FspBI CTAG 1 cut(s) 210
FspI TGCGCA 1 cut(s) 930
GlaI GCGC 2 cut(s) 920, 930
GluI GCNGC 3 cut(s) 699, 714, 783
GsuI CTGGAG 2 cut(s) 174, 738
HaeII RGCGCY 1 cut(s) 922
HaeIII GGCC 4 cut(s) 195, 367, 876, 973
HapII CCGG 2 cut(s) 72, 777
HhaI GCGC 2 cut(s) 921, 931
Hin1II CATG 1 cut(s) 625
Hin6I GCGC 2 cut(s) 919, 929
HinP1I GCGC 2 cut(s) 919, 929
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 2 cut(s) 213, 563
HpaII CCGG 2 cut(s) 72, 777
HphI GGTGA 3 cut(s) 163, 164, 443
Hpy188I TCNGA 1 cut(s) 833
Hpy188III TCNNGA 4 cut(s) 255, 372, 753, 941
HpyAV CCTTC 6 cut(s) 117, 206, 230, 350, 378, 718
HpyCH4III ACNGT 2 cut(s) 310, 768
HpyCH4IV ACGT 1 cut(s) 481
HpyCH4V TGCA 4 cut(s) 239, 643, 740, 995
HpyF10VI GCNNNNNNNGC 2 cut(s) 782, 935
HpyF3I CTNAG 1 cut(s) 830
HpySE526I ACGT 1 cut(s) 481
Hsp92II CATG 1 cut(s) 625
HspAI GCGC 2 cut(s) 919, 929
KpnI GGTACC 1 cut(s) 669
Kzo9I GATC 5 cut(s) 83, 486, 607, 958, 1013
Lsp1109I GCAGC 2 cut(s) 710, 769
LweI GCATC 3 cut(s) 381, 630, 982
MaeI CTAG 1 cut(s) 210
MaeII ACGT 1 cut(s) 481
MalI GATC 5 cut(s) 85, 488, 609, 960, 1015
MboI GATC 5 cut(s) 83, 486, 607, 958, 1013
MboII GAAGA 6 cut(s) 85, 105, 481, 524, 558, 698
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 2 cut(s) 379, 954
MluCI AATT 5 cut(s) 35, 528, 691, 756, 834
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 2 cut(s) 12, 364
MnlI CCTC 6 cut(s) 127, 234, 237, 427, 928, 1008
MseI TTAA 6 cut(s) 260, 330, 852, 894, 905, 924
MspA1I CMGCKG 2 cut(s) 716, 1011
MspCI CTTAAG 1 cut(s) 904
MspI CCGG 2 cut(s) 72, 777
MspR9I CCNGG 5 cut(s) 192, 349, 429, 600, 778
Mva1269I GAATGC 1 cut(s) 469
MvaI CCWGG 4 cut(s) 192, 349, 429, 600
MwoI GCNNNNNNNGC 2 cut(s) 782, 935
NciI CCSGG 1 cut(s) 778
NdeII GATC 5 cut(s) 83, 486, 607, 958, 1013
NlaIII CATG 1 cut(s) 625
NlaIV GGNNCC 1 cut(s) 667
NsbI TGCGCA 1 cut(s) 930
NspV TTCGAA 1 cut(s) 569
PceI AGGCCT 2 cut(s) 195, 367
PctI GAATGC 1 cut(s) 469
PfeI GAWTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 676
PkrI GCNGC 3 cut(s) 700, 715, 784
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
Psp6I CCWGG 4 cut(s) 190, 347, 427, 598
PspGI CCWGG 4 cut(s) 190, 347, 427, 598
PspN4I GGNNCC 1 cut(s) 667
PspPI GGNCC 1 cut(s) 596
PsuI RGATCY 1 cut(s) 607
RsaI GTAC 4 cut(s) 280, 548, 587, 667
RsaNI GTAC 4 cut(s) 279, 547, 586, 666
SaqAI TTAA 6 cut(s) 260, 330, 852, 894, 905, 924
SatI GCNGC 3 cut(s) 699, 714, 783
Sau3AI GATC 5 cut(s) 83, 486, 607, 958, 1013
Sau96I GGNCC 1 cut(s) 596
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 5 cut(s) 192, 349, 429, 600, 778
SduI GDGCHC 2 cut(s) 379, 954
SetI ASST 8 cut(s) 211, 267, 280, 484, 552, 849, 1000, 1021
SfaNI GCATC 3 cut(s) 381, 630, 982
SfcI CTRYAG 3 cut(s) 396, 418, 764
SfuI TTCGAA 1 cut(s) 569
SinI GGWCC 1 cut(s) 596
SmlI CTYRAG 1 cut(s) 904
SmoI CTYRAG 1 cut(s) 904
Sse9I AATT 5 cut(s) 35, 528, 691, 756, 834
SseBI AGGCCT 2 cut(s) 195, 367
SsiI CCGC 3 cut(s) 81, 714, 1011
SspMI CTAG 1 cut(s) 210
StuI AGGCCT 2 cut(s) 195, 367
StyD4I CCNGG 5 cut(s) 190, 347, 427, 598, 776
StyI CCWWGG 1 cut(s) 1002
TaaI ACNGT 2 cut(s) 310, 768
TaiI ACGT 1 cut(s) 484
TaqI TCGA 1 cut(s) 569
TasI AATT 5 cut(s) 35, 528, 691, 756, 834
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 716
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 6 cut(s) 260, 330, 852, 894, 905, 924
Tru9I TTAA 6 cut(s) 260, 330, 852, 894, 905, 924
TseI GCWGC 2 cut(s) 698, 782
TspDTI ATGAA 7 cut(s) 188, 481, 525, 828, 837, 881, 929
TspGWI ACGGA 1 cut(s) 336
Van91I CCANNNNNTGG 1 cut(s) 676
Vha464I CTTAAG 1 cut(s) 904
VpaK11BI GGWCC 1 cut(s) 596
XapI RAATTY 1 cut(s) 528
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.