pycom06g07990

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006314
Physical Location & Seq
Forward (+)
13040789 .. 13043195
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2169 bp
ATGGATTTAGATAGAAATTGCACGGTAGGTTTGAGTCCTAACACTGTGCTTCCAACTCATTGTCCTCATTCCCATGGTGAAAGAAGGAGCACAAAAGGAAAATTAAAACGTAAAGATGATTTATCGAGCATAAAGGAGGATTTCGTGGAAATCAGCTTTCATCACTATCGCAGCGCTTCTTGTAAAAGTGTTCCATCTAGGCCTGTTGTTAACGTAGACCTTAAGCGTGGTTCTATATATCAAAGCTCCAAAGAGGTACAAAATATTAAGAAAATGGGCACTGTTGAAGGAAGGAGAAAAGTTGGAATGCCGCGAAGTAGTGACACCTCTTTCTCTTACCGAATTGTTGACACGATGTGTAGTTCAGATGAGGAGAGTACACAGAATAGGTCTTCAGTAGTATCTTTGAAATCAGACTTGAATGCACCATCTGTTGGCCGGCCTCATGTTGAACCATGCTCATCAGATAGTTTGATTGATATTTGTATGGATCCCGATTACAGGGAAAAGCACTCTGCTGAAGCTTTACGAGAATTCTCTGTGGATCTGAAACTAAGAAGTGATGCTGTTGCTGGACCTCTAAATAATGGTAATGAACTTCTGGAAAGAGACCAAGTTCACACATTGCACAAGTCTTTCTCCGCTAAGGTGGAAATGCTCCAATCTCGTTCCCCACCAGAGAGTGCTCGCTCATCCAGGGTCAGCTCAAAAACCCGGTTCAGCCCTATTATAAAGATGTTTGATCCATTCATGAAGTCTAAGTCTTTGAGAACTCCGAGTTATGTAGTGGAACCTGGCAGAGCCAAAACGACTGAGCCGGCAAACATGACAAGAAACAGGTCACATAAAAAATCTTTGTTGCCAGTCTATTCAAATACAGCACAGAGTCCAGATTGTGATCCTCCGCGTATCAACCAAGATAGCCACCAATCGGTTGTTGCATGCTCGCCAGTTCACCTACATGGCCATCTCAAGTTACAAAATAAATACGGAATGCCATTTTTTGAGTTCTCGTTGAAGTGCTCAGGAGACGTCTTGGTGGCCAGGACATGGAAAGCAGATAATGCTATTAATTGGGTGTATACCTTTCACTCCATCGGAAGCAGAAAGAAGAGCAATGCCAGCGGTTGGGGATTATATGGTAGTGACAGAGATTCTTCAATGGTGGGACAGATGCAAGTGTCCTGTTACCTATGTTCGGAACTCAAAGATGGAGTTTTTGAAAATTCCATGGTGACGGAGTTTGTGGTGTATGATATCGCTCATGCGAGGCAAACGTTTGCAGCCCAAGAAAACTCTAATAGTAGCCTTGATGATGTTAAACCTCCCAAGGGTTCTATTCCAGGCACAACTGTAAAGTTGGATGAGTCTTGTCCCACCAAGGTCAAACTTCAACAAAAACATGCTTCCAACAGCAGTGATAATAATACTTCAGCCTGGCCGTCTGCAGATTTGCATCCAAGCCTTGAAATCGCAGCCATCGTTATGCAAGTTCCTTTAGAAAAGAGAGAGAGCTTGAAATACAAGAGAGGGGATAAAATTAGTGATAAAGTACATCGAAACCTACTGTCCATGGGTGAACAGAAGAAGGAGGCTACCCCTGATAGCCAAAGTCCAGGAAACTTACAGGTGGTGGTACCAGCTGGAAACCATGGCACACCGAGTGCTGAAAGCAGCGGTCCTTCATCATTACTAGATCGGTGGAGGTTGGGTGGAGGCTGCGACTGTGGTGGCTGGGACATGTCCTGTCCTCTTATTGTCTTAAGCAATCCCCACACCAAAAGCGCTGATAATCAACTATTGGCAGAGAATCAGAAGCTTATGGAACTTTATGTTCAGGGATCGAAGGAGAAAGCACCAGCAGTGATCATGACAATGGTTGAAGAGGGACACTATGCAGTTGATTTTCATGCCCGGTTATCTACACTACAAGCATTTTCTACATGTGTTGCCATTTTGCACGGTTCAGAAACATCCGCAGCCGCAGGGCAGGAGAGGAACACACAGTTGTCGCAATGCAGCTCATTGAAAGTAGTGCTTGAGGAAGAAGTGAAGTACTTAATTGAAGCAGTTACGGCAGGGGAGAAGAAGGAAGTAGCTAAGAGGGTGAAAAAAATCCAACCGTCTTATGTACTGAACCCTCCCTTCTCTCCAATTGCTCGGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BAH DUF3527 TFIIS_M

Protein Analysis

723

Amino Acids

79.63

Weight (kDa)

8.76

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 317 - 655 2.4e-110 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
AatII GACGTC 1 cut(s) 1035
Acc65I GGTACC 1 cut(s) 1636
AccB1I GGYRCC 1 cut(s) 1636
AccB7I CCANNNNNTGG 3 cut(s) 434, 1050, 1128
AccI GTMKAC 2 cut(s) 216, 1082
AccII CGCG 2 cut(s) 313, 907
AciI CCGC 7 cut(s) 311, 642, 905, 1125, 1677, 1977, 1983
AclI AACGTT 1 cut(s) 1277
AclWI GGATC 6 cut(s) 485, 498, 552, 737, 893, 1849
AcoI YGGCCR 4 cut(s) 436, 964, 1041, 1439
AcsI RAATTY 2 cut(s) 533, 1225
AcuI CTGAAG 3 cut(s) 378, 540, 1416
AcyI GRCGYC 1 cut(s) 1032
AdeI CACNNNGTG 2 cut(s) 357, 1664
AfaI GTAC 6 cut(s) 258, 379, 1554, 1638, 2057, 2133
AfeI AGCGCT 2 cut(s) 175, 1786
AfiI CCNNNNNNNGG 7 cut(s) 434, 501, 931, 1050, 1128, 1198, 1331
AflII CTTAAG 2 cut(s) 221, 1762
AflIII ACRYGT 2 cut(s) 1740, 1943
AjnI CCWGG 6 cut(s) 695, 793, 1043, 1342, 1436, 1615
AluBI AGCT 9 cut(s) 156, 246, 524, 705, 1515, 1643, 1819, 2022, 2099
AluI AGCT 9 cut(s) 156, 246, 524, 705, 1515, 1643, 1819, 2022, 2099
Alw21I GWGCWC 3 cut(s) 92, 688, 1025
Alw26I GTCTC 2 cut(s) 603, 1023
AlwI GGATC 6 cut(s) 485, 498, 552, 737, 893, 1849
Ama87I CYCGRG 1 cut(s) 2160
Aor51HI AGCGCT 2 cut(s) 175, 1786
AoxI GGCC 6 cut(s) 200, 436, 440, 964, 1041, 1439
ApeKI GCWGC 7 cut(s) 171, 1283, 1475, 1674, 1719, 1979, 2019
ApoI RAATTY 2 cut(s) 533, 1225
AseI ATTAAT 1 cut(s) 1071
Asp718I GGTACC 1 cut(s) 1636
AspLEI GCGC 2 cut(s) 176, 1787
AspS9I GGNCC 2 cut(s) 575, 1679
AsuC2I CCSGG 2 cut(s) 715, 1915
AsuHPI GGTGA 5 cut(s) 89, 947, 1246, 1589, 2119
AvaI CYCGRG 1 cut(s) 2160
AvaII GGWCC 2 cut(s) 575, 1679
BaeGI GKGCMC 1 cut(s) 281
BalI TGGCCA 2 cut(s) 966, 1043
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 1636
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 3 cut(s) 92, 688, 1025
BbvI GCAGC 7 cut(s) 183, 1295, 1487, 1686, 1706, 1991, 2031
BccI CCATC 6 cut(s) 202, 436, 975, 1103, 1205, 1487
BceAI ACGGC 2 cut(s) 1426, 2091
BciT130I CCWGG 6 cut(s) 697, 795, 1045, 1344, 1438, 1617
BclI TGATCA 1 cut(s) 1866
BcnI CCSGG 2 cut(s) 715, 1915
BcoDI GTCTC 2 cut(s) 603, 1023
BfaI CTAG 2 cut(s) 198, 1694
BfmI CTRYAG 1 cut(s) 1446
BfoI RGCGCY 2 cut(s) 177, 1788
BfrI CTTAAG 2 cut(s) 221, 1762
BisI GCNGC 9 cut(s) 172, 311, 1284, 1476, 1675, 1720, 1980, 1983, 2020
BlsI GCNGC 9 cut(s) 173, 312, 1285, 1477, 1676, 1721, 1981, 1984, 2021
BmcAI AGTACT 1 cut(s) 2057
Bme1390I CCNGG 8 cut(s) 697, 715, 795, 1045, 1344, 1438, 1617, 1915
Bme18I GGWCC 2 cut(s) 575, 1679
BmeT110I CYCGRG 1 cut(s) 2160
BmgT120I GGNCC 2 cut(s) 575, 1679
BmiI GGNNCC 3 cut(s) 492, 792, 1638
BmrFI CCNGG 8 cut(s) 697, 715, 795, 1045, 1344, 1438, 1617, 1915
BmsI GCATC 3 cut(s) 553, 1164, 1465
BpiI GAAGAC 1 cut(s) 384
Bpu10I CCTNAGC 2 cut(s) 645, 1024
BpuEI CTTGAG 2 cut(s) 956, 2060
BpuMI CCSGG 2 cut(s) 715, 1915
BsaBI GATNNNNATC 2 cut(s) 897, 1455
BsaHI GRCGYC 1 cut(s) 1032
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 7 cut(s) 73, 696, 1230, 1329, 1380, 1572, 1651
BsaXI ACNNNNNCTCC 2 cut(s) 1696, 1726
Bsc4I CCNNNNNNNGG 7 cut(s) 434, 501, 931, 1050, 1128, 1198, 1331
Bse118I RCCGGY 2 cut(s) 438, 817
Bse1I ACTGG 2 cut(s) 863, 950
Bse3DI GCAATG 3 cut(s) 623, 1123, 2021
Bse8I GATNNNNATC 2 cut(s) 897, 1455
BseBI CCWGG 6 cut(s) 697, 795, 1045, 1344, 1438, 1617
BseDI CCNNGG 7 cut(s) 73, 696, 1230, 1329, 1380, 1572, 1651
BseGI GGATG 4 cut(s) 692, 1369, 1456, 1973
BseJI GATNNNNATC 2 cut(s) 897, 1455
BseLI CCNNNNNNNGG 7 cut(s) 434, 501, 931, 1050, 1128, 1198, 1331
BseMI GCAATG 3 cut(s) 623, 1123, 2021
BseMII CTCAG 2 cut(s) 804, 1038
BseNI ACTGG 2 cut(s) 863, 950
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 1 cut(s) 281
BseXI GCAGC 7 cut(s) 183, 1295, 1487, 1686, 1706, 1991, 2031
BseYI CCCAGC 1 cut(s) 1734
Bsh1236I CGCG 2 cut(s) 313, 907
BshFI GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1441
BshNI GGYRCC 1 cut(s) 1636
BsiHKAI GWGCWC 3 cut(s) 92, 688, 1025
BsiHKCI CYCGRG 1 cut(s) 2160
BsiSI CCGG 4 cut(s) 439, 715, 818, 1915
BslFI GGGAC 4 cut(s) 1182, 1359, 1751, 1902
BslI CCNNNNNNNGG 7 cut(s) 434, 501, 931, 1050, 1128, 1198, 1331
BsmAI GTCTC 2 cut(s) 603, 1023
BsmBI CGTCTC 1 cut(s) 1023
BsmFI GGGAC 4 cut(s) 1182, 1359, 1751, 1902
BsmI GAATGC 3 cut(s) 312, 427, 999
BsnI GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1441
Bso31I GGTCTC 1 cut(s) 603
BsoBI CYCGRG 1 cut(s) 2160
Bsp1286I GDGCHC 4 cut(s) 92, 281, 688, 1025
Bsp143I GATC 7 cut(s) 490, 544, 742, 898, 1696, 1841, 1866
Bsp19I CCATGG 4 cut(s) 73, 1230, 1572, 1651
BspACI CCGC 7 cut(s) 311, 642, 905, 1125, 1677, 1977, 1983
BspANI GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1441
BspCNI CTCAG 2 cut(s) 805, 1037
BspFNI CGCG 2 cut(s) 313, 907
BspHI TCATGA 2 cut(s) 750, 1869
BspLI GGNNCC 3 cut(s) 492, 792, 1638
BspMAI CTGCAG 1 cut(s) 1450
BspPI GGATC 6 cut(s) 485, 498, 552, 737, 893, 1849
BspQI GCTCTTC 1 cut(s) 1106
BspT107I GGYRCC 1 cut(s) 1636
BspTI CTTAAG 2 cut(s) 221, 1762
BspTNI GGTCTC 1 cut(s) 603
BsrDI GCAATG 3 cut(s) 623, 1123, 2021
BsrFI RCCGGY 2 cut(s) 438, 817
BsrI ACTGG 2 cut(s) 863, 950
BssAI RCCGGY 2 cut(s) 438, 817
BssECI CCNNGG 7 cut(s) 73, 696, 1230, 1329, 1380, 1572, 1651
BssMI GATC 7 cut(s) 490, 544, 742, 898, 1696, 1841, 1866
BssNAI GTATAC 1 cut(s) 1083
BssNI GRCGYC 1 cut(s) 1032
BssT1I CCWWGG 6 cut(s) 73, 1230, 1329, 1380, 1572, 1651
Bst1107I GTATAC 1 cut(s) 1083
Bst2UI CCWGG 6 cut(s) 697, 795, 1045, 1344, 1438, 1617
Bst4CI ACNGT 9 cut(s) 25, 46, 283, 1354, 1569, 1727, 1964, 2007, 2124
Bst6I CTCTTC 2 cut(s) 1106, 1878
BstACI GRCGYC 1 cut(s) 1032
BstAFI CTTAAG 2 cut(s) 221, 1762
BstAPI GCANNNNNTGC 1 cut(s) 1064
BstC8I GCNNGC 6 cut(s) 440, 688, 819, 943, 947, 1123
BstDEI CTNAG 6 cut(s) 554, 645, 759, 813, 1024, 2100
BstDSI CCRYGG 4 cut(s) 73, 1230, 1572, 1651
BstF5I GGATG 4 cut(s) 692, 1369, 1456, 1973
BstFNI CGCG 2 cut(s) 313, 907
BstH2I RGCGCY 2 cut(s) 177, 1788
BstHHI GCGC 2 cut(s) 176, 1787
BstKTI GATC 7 cut(s) 493, 547, 745, 901, 1699, 1844, 1869
BstMAI GTCTC 2 cut(s) 603, 1023
BstMBI GATC 7 cut(s) 490, 544, 742, 898, 1696, 1841, 1866
BstMWI GCNNNNNNNGC 3 cut(s) 1064, 1122, 2075
BstNI CCWGG 6 cut(s) 697, 795, 1045, 1344, 1438, 1617
BstNSI RCATGY 4 cut(s) 945, 1406, 1744, 1947
BstSCI CCNGG 8 cut(s) 695, 713, 793, 1043, 1342, 1436, 1615, 1913
BstSFI CTRYAG 1 cut(s) 1446
BstSLI GKGCMC 1 cut(s) 281
BstUI CGCG 2 cut(s) 313, 907
BstV1I GCAGC 7 cut(s) 183, 1295, 1487, 1686, 1706, 1991, 2031
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 2 cut(s) 490, 544
BstYI RGATCY 2 cut(s) 490, 544
BstZ17I GTATAC 1 cut(s) 1083
BsuRI GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1441
BtgI CCRYGG 4 cut(s) 73, 1230, 1572, 1651
BtsCI GGATG 4 cut(s) 692, 1369, 1456, 1973
BtsI GCAGTG 2 cut(s) 1423, 1869
BtsIMutI CAGTG 4 cut(s) 42, 279, 1423, 1869
Cac8I GCNNGC 6 cut(s) 440, 688, 819, 943, 947, 1123
CciI TCATGA 2 cut(s) 750, 1869
CfoI GCGC 2 cut(s) 176, 1787
Cfr10I RCCGGY 2 cut(s) 438, 817
Cfr13I GGNCC 2 cut(s) 575, 1679
Csp6I GTAC 6 cut(s) 257, 378, 1553, 1637, 2056, 2132
CviQI GTAC 6 cut(s) 257, 378, 1553, 1637, 2056, 2132
DdeI CTNAG 6 cut(s) 554, 645, 759, 813, 1024, 2100
DpnI GATC 7 cut(s) 492, 546, 744, 900, 1698, 1843, 1868
DpnII GATC 7 cut(s) 490, 544, 742, 898, 1696, 1841, 1866
DraIII CACNNNGTG 2 cut(s) 357, 1664
EaeI YGGCCR 4 cut(s) 436, 964, 1041, 1439
Eam1104I CTCTTC 2 cut(s) 1106, 1878
EarI CTCTTC 2 cut(s) 1106, 1878
Eco130I CCWWGG 6 cut(s) 73, 1230, 1329, 1380, 1572, 1651
Eco147I AGGCCT 1 cut(s) 202
Eco31I GGTCTC 1 cut(s) 603
Eco32I GATATC 1 cut(s) 1258
Eco47I GGWCC 2 cut(s) 575, 1679
Eco47III AGCGCT 2 cut(s) 175, 1786
Eco57I CTGAAG 3 cut(s) 378, 540, 1416
Eco88I CYCGRG 1 cut(s) 2160
EcoRI GAATTC 1 cut(s) 533
EcoRII CCWGG 6 cut(s) 695, 793, 1043, 1342, 1436, 1615
EcoRV GATATC 1 cut(s) 1258
EcoT14I CCWWGG 6 cut(s) 73, 1230, 1329, 1380, 1572, 1651
ErhI CCWWGG 6 cut(s) 73, 1230, 1329, 1380, 1572, 1651
Esp3I CGTCTC 1 cut(s) 1023
FalI AAGNNNNNCTT 2 cut(s) 2022, 2054
FaqI GGGAC 4 cut(s) 1182, 1359, 1751, 1902
FbaI TGATCA 1 cut(s) 1866
FblI GTMKAC 2 cut(s) 216, 1082
Fnu4HI GCNGC 9 cut(s) 172, 311, 1284, 1476, 1675, 1720, 1980, 1983, 2020
FokI GGATG 4 cut(s) 679, 1376, 1443, 1960
FseI GGCCGGCC 1 cut(s) 442
Fsp4HI GCNGC 9 cut(s) 172, 311, 1284, 1476, 1675, 1720, 1980, 1983, 2020
FspBI CTAG 2 cut(s) 198, 1694
GlaI GCGC 2 cut(s) 175, 1786
GluI GCNGC 9 cut(s) 172, 311, 1284, 1476, 1675, 1720, 1980, 1983, 2020
GsaI CCCAGC 1 cut(s) 1738
HaeII RGCGCY 2 cut(s) 177, 1788
HaeIII GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1441
HapII CCGG 4 cut(s) 439, 715, 818, 1915
HhaI GCGC 2 cut(s) 176, 1787
Hin1I GRCGYC 1 cut(s) 1032
Hin6I GCGC 2 cut(s) 174, 1785
HinP1I GCGC 2 cut(s) 174, 1785
HincII GTYRAC 2 cut(s) 211, 349
HindII GTYRAC 2 cut(s) 211, 349
HindIII AAGCTT 2 cut(s) 522, 1817
HinfI GANTC 5 cut(s) 34, 886, 1154, 1367, 1810
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 4 cut(s) 439, 715, 818, 1915
HphI GGTGA 5 cut(s) 89, 947, 1246, 1589, 2119
Hpy166II GTNNAC 8 cut(s) 211, 217, 349, 380, 619, 955, 1083, 1580
Hpy188I TCNGA 9 cut(s) 367, 415, 466, 549, 777, 1100, 1201, 1815, 1969
Hpy188III TCNNGA 6 cut(s) 494, 602, 751, 890, 1026, 1870
Hpy8I GTNNAC 8 cut(s) 211, 217, 349, 380, 619, 955, 1083, 1580
HpyAV CCTTC 8 cut(s) 78, 281, 285, 1582, 1692, 1840, 2083, 2155
HpyCH4III ACNGT 9 cut(s) 25, 46, 283, 1354, 1569, 1727, 1964, 2007, 2124
HpyCH4IV ACGT 4 cut(s) 109, 213, 1032, 1277
HpyF10VI GCNNNNNNNGC 3 cut(s) 1064, 1122, 2075
HpyF3I CTNAG 6 cut(s) 554, 645, 759, 813, 1024, 2100
HpySE526I ACGT 4 cut(s) 109, 213, 1032, 1277
Hsp92I GRCGYC 1 cut(s) 1032
HspAI GCGC 2 cut(s) 174, 1785
KpnI GGTACC 1 cut(s) 1640
KroI GCCGGC 2 cut(s) 438, 817
KroNI GCCGGC 2 cut(s) 440, 819
Ksp22I TGATCA 1 cut(s) 1866
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 7 cut(s) 490, 544, 742, 898, 1696, 1841, 1866
LguI GCTCTTC 1 cut(s) 1106
LmnI GCTCC 3 cut(s) 87, 251, 663
Lsp1109I GCAGC 7 cut(s) 183, 1295, 1487, 1686, 1706, 1991, 2031
LweI GCATC 3 cut(s) 553, 1164, 1465
MaeI CTAG 2 cut(s) 198, 1694
MaeII ACGT 4 cut(s) 109, 213, 1032, 1277
MaeIII GTNAC 7 cut(s) 320, 840, 975, 1145, 1187, 1234, 2071
MalI GATC 7 cut(s) 492, 546, 744, 900, 1698, 1843, 1868
MboI GATC 7 cut(s) 490, 544, 742, 898, 1696, 1841, 1866
MboII GAAGA 7 cut(s) 384, 1123, 1149, 1597, 1895, 2057, 2098
MfeI CAATTG 1 cut(s) 2154
MflI RGATCY 2 cut(s) 490, 544
MhlI GDGCHC 4 cut(s) 92, 281, 688, 1025
MlsI TGGCCA 2 cut(s) 966, 1043
MluCI AATT 9 cut(s) 16, 101, 342, 533, 1072, 1225, 1539, 2061, 2154
MluNI TGGCCA 2 cut(s) 966, 1043
MlyI GAGTC 3 cut(s) 43, 895, 1376
MmeI TCCRAC 5 cut(s) 77, 283, 1341, 1434, 2143
Mox20I TGGCCA 2 cut(s) 966, 1043
MroNI GCCGGC 2 cut(s) 438, 817
MscI TGGCCA 2 cut(s) 966, 1043
MseI TTAA 8 cut(s) 104, 210, 222, 267, 1071, 1320, 1763, 2060
MslI CAYNNNNRTG 4 cut(s) 72, 960, 1484, 1874
Msp20I TGGCCA 2 cut(s) 966, 1043
MspA1I CMGCKG 3 cut(s) 1125, 1643, 1677
MspCI CTTAAG 2 cut(s) 221, 1762
MspI CCGG 4 cut(s) 439, 715, 818, 1915
MspR9I CCNGG 8 cut(s) 697, 715, 795, 1045, 1344, 1438, 1617, 1915
MunI CAATTG 1 cut(s) 2154
Mva1269I GAATGC 3 cut(s) 312, 427, 999
MvaI CCWGG 6 cut(s) 697, 795, 1045, 1344, 1438, 1617
MvnI CGCG 2 cut(s) 313, 907
MwoI GCNNNNNNNGC 3 cut(s) 1064, 1122, 2075
NaeI GCCGGC 2 cut(s) 440, 819
NciI CCSGG 2 cut(s) 715, 1915
NcoI CCATGG 4 cut(s) 73, 1230, 1572, 1651
NdeII GATC 7 cut(s) 490, 544, 742, 898, 1696, 1841, 1866
NgoMIV GCCGGC 2 cut(s) 438, 817
NlaIV GGNNCC 3 cut(s) 492, 792, 1638
NmuCI GTSAC 4 cut(s) 320, 840, 1145, 1234
NspI RCATGY 4 cut(s) 945, 1406, 1744, 1947
PaeI GCATGC 1 cut(s) 945
PagI TCATGA 2 cut(s) 750, 1869
PceI AGGCCT 1 cut(s) 202
PciI ACATGT 2 cut(s) 1740, 1943
PciSI GCTCTTC 1 cut(s) 1106
PcsI WCGNNNNNNNCGW 1 cut(s) 1479
PctI GAATGC 3 cut(s) 312, 427, 999
PdiI GCCGGC 2 cut(s) 440, 819
PfeI GAWTC 2 cut(s) 1154, 1810
PflMI CCANNNNNTGG 3 cut(s) 434, 1050, 1128
PfoI TCCNGGA 1 cut(s) 1615
PkrI GCNGC 9 cut(s) 173, 312, 1285, 1477, 1676, 1721, 1981, 1984, 2021
PleI GAGTC 3 cut(s) 42, 894, 1375
PpsI GAGTC 3 cut(s) 42, 894, 1375
PscI ACATGT 2 cut(s) 1740, 1943
PshBI ATTAAT 1 cut(s) 1071
PsiI TTATAA 1 cut(s) 731
Psp1406I AACGTT 1 cut(s) 1277
Psp6I CCWGG 6 cut(s) 695, 793, 1043, 1342, 1436, 1615
PspFI CCCAGC 1 cut(s) 1734
PspGI CCWGG 6 cut(s) 695, 793, 1043, 1342, 1436, 1615
PspN4I GGNNCC 3 cut(s) 492, 792, 1638
PspPI GGNCC 2 cut(s) 575, 1679
PstI CTGCAG 1 cut(s) 1450
PsuI RGATCY 2 cut(s) 490, 544
PvuII CAGCTG 1 cut(s) 1643
RigI GGCCGGCC 1 cut(s) 442
RsaI GTAC 6 cut(s) 258, 379, 1554, 1638, 2057, 2133
RsaNI GTAC 6 cut(s) 257, 378, 1553, 1637, 2056, 2132
RseI CAYNNNNRTG 4 cut(s) 72, 960, 1484, 1874
SapI GCTCTTC 1 cut(s) 1106
SaqAI TTAA 8 cut(s) 104, 210, 222, 267, 1071, 1320, 1763, 2060
SatI GCNGC 9 cut(s) 172, 311, 1284, 1476, 1675, 1720, 1980, 1983, 2020
Sau3AI GATC 7 cut(s) 490, 544, 742, 898, 1696, 1841, 1866
Sau96I GGNCC 2 cut(s) 575, 1679
ScaI AGTACT 1 cut(s) 2057
SchI GAGTC 3 cut(s) 43, 895, 1376
ScrFI CCNGG 8 cut(s) 697, 715, 795, 1045, 1344, 1438, 1617, 1915
SduI GDGCHC 4 cut(s) 92, 281, 688, 1025
SfaNI GCATC 3 cut(s) 553, 1164, 1465
SfcI CTRYAG 1 cut(s) 1446
SinI GGWCC 2 cut(s) 575, 1679
SmiMI CAYNNNNRTG 4 cut(s) 72, 960, 1484, 1874
SmlI CTYRAG 4 cut(s) 221, 971, 1762, 2039
SmoI CTYRAG 4 cut(s) 221, 971, 1762, 2039
SphI GCATGC 1 cut(s) 945
Sse9I AATT 9 cut(s) 16, 101, 342, 533, 1072, 1225, 1539, 2061, 2154
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 7 cut(s) 311, 642, 905, 1125, 1677, 1977, 1983
SspI AATATT 1 cut(s) 265
SspMI CTAG 2 cut(s) 198, 1694
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 8 cut(s) 695, 713, 793, 1043, 1342, 1436, 1615, 1913
StyI CCWWGG 6 cut(s) 73, 1230, 1329, 1380, 1572, 1651
TaaI ACNGT 9 cut(s) 25, 46, 283, 1354, 1569, 1727, 1964, 2007, 2124
TaiI ACGT 4 cut(s) 112, 216, 1035, 1280
TaqI TCGA 3 cut(s) 125, 1558, 1844
TasI AATT 9 cut(s) 16, 101, 342, 533, 1072, 1225, 1539, 2061, 2154
TatI WGTACW 4 cut(s) 377, 1552, 2055, 2131
TauI GCSGC 2 cut(s) 313, 1985
TfiI GAWTC 2 cut(s) 1154, 1810
Tru1I TTAA 8 cut(s) 104, 210, 222, 267, 1071, 1320, 1763, 2060
Tru9I TTAA 8 cut(s) 104, 210, 222, 267, 1071, 1320, 1763, 2060
TscAI CASTG 4 cut(s) 49, 286, 1423, 1869
TseFI GTSAC 4 cut(s) 320, 840, 1145, 1234
TseI GCWGC 7 cut(s) 171, 1283, 1475, 1674, 1719, 1979, 2019
Tsp45I GTSAC 4 cut(s) 320, 840, 1145, 1234
TspDTI ATGAA 6 cut(s) 149, 609, 739, 767, 1674, 1898
TspGWI ACGGA 2 cut(s) 1005, 1253
TspRI CASTG 4 cut(s) 49, 286, 1423, 1869
Van91I CCANNNNNTGG 3 cut(s) 434, 1050, 1128
Vha464I CTTAAG 2 cut(s) 221, 1762
VpaK11BI GGWCC 2 cut(s) 575, 1679
VspI ATTAAT 1 cut(s) 1071
XapI RAATTY 2 cut(s) 533, 1225
XceI RCATGY 4 cut(s) 945, 1406, 1744, 1947
XmiI GTMKAC 2 cut(s) 216, 1082
XspI CTAG 2 cut(s) 198, 1694
ZraI GACGTC 1 cut(s) 1033
ZrmI AGTACT 1 cut(s) 2057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.