pycom06g14060

Kelch domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006640
Physical Location & Seq
Reverse (-)
18940408 .. 18944400
3993 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1380 bp
ATGGGGAAGAAGACAAAGAAGCCAGGTAAAGGAAAAGAGAAGACGGAGAAGAAAACTGCGAAGGCTGATGAGAAGAGAGCTCGCAGAGAGACCAAGAAGCTCTCTCCCGAGGACGACATCGATGCCATTTTGTCGAGTATACAAAAAGAAGAAGCTAAGAAGAAGGAAGTCCATGTAGAGGAAAATGTCCCGGCTCCATCTCCACGGTCCAACTGCACGACGTTTGTGTATGGTGATCTTTACCGCTATGATGTTGAAAAGCAGGACTGGAAGTTAATTTCGAGCCCCAATAGTCCACCTCCTCGCAGTGCGCACCAGGCGGTCACTTGGAAGAACTATCTCTACATCTTTGGTGGTGAGTTTACTTCCCCAAACCAAGAGAGGTTCCATCACTACAAGGACTTTTGGATGTTGGACCTCAAAACAAATCAATGGGAACTACTAAATTTAAAAGGGAGTCCAAGTCCACGCTCAGGTCATCGAATGGTACTGTACAAGCACAAGATTATTGTTTTTGGAGGCTTTTATGACACGCTTAGAGAAGTGAGGTATCACAATGATCTATTTGTATTTGACCTGGATCAGTTCAAGTGGCAAGAAATAACTCCTAGGCCTGGTTCCTTGTGGCCAAGTGCCCGAAGTGGTTTTCAGTTTTTTGTGTACCAAGATGAAATTTTCTTATATGGTGGCTATTCCAAAGAAGTTTCATCTGATAAAACTGGCTCCGAGAAGGGAATTGTTCATTCAGACTTGTGGTCCCTTGATCCTAGGACTTGGGAATGGAACAAGGTGAAGAAAAGTGGGATGCCTCCTGGACCTCGTGCTGGGTTCTCTATGTGTGTTCATAAGAAGCGAGCTTTGCTCTTCGGGGGTGTGGTGGACATGGAAGTTGGAGCTGATGTAATGATGAGCTTGTTCTTGGACGAACTGTATGGCTTCCAATTGGACAACCATCGGTGGTACCCATTAGAGCTACGGAAAGCGAAGTCAACAAAAGATAAGCCTAAAAAGAGTTCTGGACGAAAGCGTAAAAATGATGATAAGATAAATTCAATTGAGCCAGAGGAATGTGCGACAAATGAAAAAGATGAGAACAATGAGATTGATGAAGAAGCAGATGACTTTGAAAGCACAGTTGATGGTATATCACATCAAATGGCTAAAACCATTACTGTTGGTAATGATGGGTTAGCTGAAGAATCTGATGGGAAGATAAATGAATCTAGTGCTAATTTGGATTCACAAGATTCTGATTTGCCAGAGGTAGTGAAGCCTTGTGGACGTATCAATTCTTGCATGGTTGTTGGAAGAGATACATTATATGTTTATGGGGGAATGATGGAGATTAAATGGATCGGATCGAGAAATCACACTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4110 Kelch_3 Kelch_4 Kelch_5

Protein Analysis

460

Amino Acids

52.68

Weight (kDa)

7.19

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 75 - 234 3.8e-34 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 77 - 291 3e-48 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 78 - 150 1.9e-13 Attractin/LZTR1 beta-propeller
NANM PF24996 80 - 201 7.4e-09 N-acetylneuraminate epimerase
Kelch_FKB95 PF25210 87 - 240 6.1e-08 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 152 - 204 2e-09 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 156 - 203 3.3e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 185 - 266 5.4e-10 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 210 - 327 1.2e-16 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 244 - 326 3.8e-12 FBX42, beta-propeller domain
Kelch_HCF PF13854 245 - 314 9.7e-06 Host cell factor, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 312
Acc65I GGTACC 1 cut(s) 960
AccB1I GGYRCC 1 cut(s) 960
AccI GTMKAC 1 cut(s) 139
AciI CCGC 2 cut(s) 244, 320
AclWI GGATC 4 cut(s) 588, 758, 1361, 1366
AcoI YGGCCR 1 cut(s) 626
AcsI RAATTY 3 cut(s) 445, 672, 1048
AcuI CTGAAG 1 cut(s) 1215
AfaI GTAC 4 cut(s) 489, 494, 662, 962
AfiI CCNNNNNNNGG 5 cut(s) 29, 178, 473, 614, 824
AgsI TTSAA 4 cut(s) 257, 589, 1053, 1127
AhdI GACNNNNNGTC 1 cut(s) 754
AjnI CCWGG 5 cut(s) 22, 315, 576, 613, 811
AluBI AGCT 8 cut(s) 80, 100, 155, 857, 896, 912, 973, 1193
AluI AGCT 8 cut(s) 80, 100, 155, 857, 896, 912, 973, 1193
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 4 cut(s) 588, 758, 1361, 1366
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 2 cut(s) 611, 626
ApoI RAATTY 3 cut(s) 445, 672, 1048
Asp718I GGTACC 1 cut(s) 960
AspA2I CCTAGG 2 cut(s) 608, 767
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 4 cut(s) 207, 415, 756, 815
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 3 cut(s) 245, 368, 802
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 4 cut(s) 207, 415, 756, 815
AvrII CCTAGG 2 cut(s) 608, 767
BaeGI GKGCMC 1 cut(s) 637
BalI TGGCCA 1 cut(s) 628
BanI GGYRCC 1 cut(s) 960
BanII GRGCYC 2 cut(s) 82, 287
BauI CACGAG 1 cut(s) 819
BbsI GAAGAC 2 cut(s) 17, 47
Bbv12I GWGCWC 1 cut(s) 82
BccI CCATC 7 cut(s) 205, 396, 960, 1133, 1178, 1199, 1333
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 5 cut(s) 24, 317, 578, 615, 813
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 3 cut(s) 609, 768, 1224
BlnI CCTAGG 2 cut(s) 608, 767
Bme1390I CCNGG 6 cut(s) 24, 191, 317, 578, 615, 813
Bme18I GGWCC 4 cut(s) 207, 415, 756, 815
BmeRI GACNNNNNGTC 1 cut(s) 754
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 4 cut(s) 207, 415, 756, 815
BmiI GGNNCC 6 cut(s) 195, 386, 619, 724, 758, 962
BmrFI CCNGG 6 cut(s) 24, 191, 317, 578, 615, 813
BmsI GCATC 2 cut(s) 112, 795
BpiI GAAGAC 2 cut(s) 17, 47
BplI GAGNNNNNCTC 2 cut(s) 846, 878
Bpu10I CCTNAGC 1 cut(s) 472
BpuMI CCSGG 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 4 cut(s) 108, 203, 608, 767
BsaXI ACNNNNNCTCC 4 cut(s) 448, 478, 885, 915
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 178, 473, 614, 824
Bse1I ACTGG 2 cut(s) 272, 724
BseBI CCWGG 5 cut(s) 24, 317, 578, 615, 813
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 4 cut(s) 108, 203, 608, 767
BseGI GGATG 2 cut(s) 414, 810
BseLI CCNNNNNNNGG 5 cut(s) 29, 178, 473, 614, 824
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 2 cut(s) 272, 724
BseRI GAGGAG 1 cut(s) 291
BseSI GKGCMC 1 cut(s) 637
BseYI CCCAGC 1 cut(s) 824
BsgI GTGCAG 1 cut(s) 199
BshFI GGCC 2 cut(s) 613, 628
BshNI GGYRCC 1 cut(s) 960
BshVI ATCGAT 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 1 cut(s) 191
BslFI GGGAC 2 cut(s) 173, 742
BslI CCNNNNNNNGG 5 cut(s) 29, 178, 473, 614, 824
BsmAI GTCTC 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 173, 742
BsnI GGCC 2 cut(s) 613, 628
Bso31I GGTCTC 1 cut(s) 83
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 3 cut(s) 82, 287, 637
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 6 cut(s) 235, 559, 580, 763, 1353, 1358
BspACI CCGC 2 cut(s) 244, 320
BspANI GGCC 2 cut(s) 613, 628
BspCNI CTCAG 1 cut(s) 485
BspDI ATCGAT 1 cut(s) 120
BspLI GGNNCC 6 cut(s) 195, 386, 619, 724, 758, 962
BspPI GGATC 4 cut(s) 588, 758, 1361, 1366
BspQI GCTCTTC 1 cut(s) 869
BspT107I GGYRCC 1 cut(s) 960
BspTNI GGTCTC 1 cut(s) 83
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 272, 724
BssECI CCNNGG 4 cut(s) 108, 203, 608, 767
BssMI GATC 6 cut(s) 235, 559, 580, 763, 1353, 1358
BssNAI GTATAC 1 cut(s) 140
BssSI CACGAG 1 cut(s) 819
BssT1I CCWWGG 2 cut(s) 608, 767
Bst1107I GTATAC 1 cut(s) 140
Bst2BI CACGAG 1 cut(s) 819
Bst2UI CCWGG 5 cut(s) 24, 317, 578, 615, 813
Bst4CI ACNGT 5 cut(s) 207, 492, 930, 1135, 1174
Bst6I CTCTTC 3 cut(s) 68, 869, 1303
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 82, 855
BstDEI CTNAG 3 cut(s) 156, 472, 536
BstDSI CCRYGG 1 cut(s) 203
BstF5I GGATG 2 cut(s) 414, 810
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 6 cut(s) 238, 562, 583, 766, 1356, 1361
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 6 cut(s) 235, 559, 580, 763, 1353, 1358
BstMWI GCNNNNNNNGC 2 cut(s) 317, 859
BstNI CCWGG 5 cut(s) 24, 317, 578, 615, 813
BstSCI CCNGG 6 cut(s) 22, 189, 315, 576, 613, 811
BstSLI GKGCMC 1 cut(s) 637
BstV2I GAAGAC 2 cut(s) 17, 47
BstZ17I GTATAC 1 cut(s) 140
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 2 cut(s) 613, 628
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 203
BtsCI GGATG 2 cut(s) 414, 810
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 1 cut(s) 313
Cac8I GCNNGC 2 cut(s) 82, 855
CfoI GCGC 1 cut(s) 313
Cfr13I GGNCC 4 cut(s) 207, 415, 756, 815
ClaI ATCGAT 1 cut(s) 120
Csp6I GTAC 4 cut(s) 488, 493, 661, 961
CviAII CATG 3 cut(s) 173, 883, 1297
CviQI GTAC 4 cut(s) 488, 493, 661, 961
DdeI CTNAG 3 cut(s) 156, 472, 536
DpnI GATC 6 cut(s) 237, 561, 582, 765, 1355, 1360
DpnII GATC 6 cut(s) 235, 559, 580, 763, 1353, 1358
DraI TTTAAA 1 cut(s) 450
DriI GACNNNNNGTC 1 cut(s) 754
EaeI YGGCCR 1 cut(s) 626
Eam1104I CTCTTC 3 cut(s) 68, 869, 1303
Eam1105I GACNNNNNGTC 1 cut(s) 754
EarI CTCTTC 3 cut(s) 68, 869, 1303
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 608, 767
Eco147I AGGCCT 1 cut(s) 613
Eco24I GRGCYC 2 cut(s) 82, 287
Eco31I GGTCTC 1 cut(s) 83
Eco47I GGWCC 4 cut(s) 207, 415, 756, 815
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 1215
Eco88I CYCGRG 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 80
EcoRII CCWGG 5 cut(s) 22, 315, 576, 613, 811
EcoT14I CCWWGG 2 cut(s) 608, 767
EcoT38I GRGCYC 2 cut(s) 82, 287
ErhI CCWWGG 2 cut(s) 608, 767
FaeI CATG 3 cut(s) 176, 886, 1300
FaqI GGGAC 2 cut(s) 173, 742
FatI CATG 3 cut(s) 172, 882, 1296
FblI GTMKAC 1 cut(s) 139
FokI GGATG 2 cut(s) 421, 817
FriOI GRGCYC 2 cut(s) 82, 287
FspAI RTGCGCAY 1 cut(s) 312
FspBI CTAG 3 cut(s) 609, 768, 1224
FspI TGCGCA 1 cut(s) 312
GlaI GCGC 1 cut(s) 312
GsaI CCCAGC 1 cut(s) 828
HaeIII GGCC 2 cut(s) 613, 628
HapII CCGG 1 cut(s) 191
HhaI GCGC 1 cut(s) 313
Hin1II CATG 3 cut(s) 176, 886, 1300
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 990
HindII GTYRAC 1 cut(s) 990
HinfI GANTC 5 cut(s) 457, 1199, 1220, 1238, 1247
HpaII CCGG 1 cut(s) 191
HphI GGTGA 3 cut(s) 245, 368, 802
Hpy166II GTNNAC 8 cut(s) 140, 296, 363, 467, 661, 880, 990, 1280
Hpy188I TCNGA 6 cut(s) 712, 727, 748, 1204, 1252, 1358
Hpy188III TCNNGA 3 cut(s) 107, 1017, 1362
Hpy8I GTNNAC 8 cut(s) 140, 296, 363, 467, 661, 880, 990, 1280
Hpy99I CGWCG 1 cut(s) 223
HpyAV CCTTC 3 cut(s) 55, 157, 724
HpyCH4III ACNGT 5 cut(s) 207, 492, 930, 1135, 1174
HpyCH4IV ACGT 2 cut(s) 221, 1282
HpyCH4V TGCA 2 cut(s) 216, 1296
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 859
HpyF3I CTNAG 3 cut(s) 156, 472, 536
HpySE526I ACGT 2 cut(s) 221, 1282
Hsp92II CATG 3 cut(s) 176, 886, 1300
HspAI GCGC 1 cut(s) 311
KpnI GGTACC 1 cut(s) 964
Kzo9I GATC 6 cut(s) 235, 559, 580, 763, 1353, 1358
LguI GCTCTTC 1 cut(s) 869
LmnI GCTCC 3 cut(s) 199, 728, 893
LweI GCATC 2 cut(s) 112, 795
MaeI CTAG 3 cut(s) 609, 768, 1224
MaeII ACGT 2 cut(s) 221, 1282
MaeIII GTNAC 1 cut(s) 322
MalI GATC 6 cut(s) 237, 561, 582, 765, 1355, 1360
MboI GATC 6 cut(s) 235, 559, 580, 763, 1353, 1358
MfeI CAATTG 2 cut(s) 941, 1053
MhlI GDGCHC 3 cut(s) 82, 287, 637
MlsI TGGCCA 1 cut(s) 628
MluCI AATT 9 cut(s) 276, 445, 672, 735, 941, 1048, 1053, 1231, 1288
MluNI TGGCCA 1 cut(s) 628
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 4 cut(s) 234, 393, 871, 1285
Mox20I TGGCCA 1 cut(s) 628
MscI TGGCCA 1 cut(s) 628
MseI TTAA 3 cut(s) 275, 449, 1347
Msp20I TGGCCA 1 cut(s) 628
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 6 cut(s) 24, 191, 317, 578, 615, 813
MunI CAATTG 2 cut(s) 941, 1053
MvaI CCWGG 5 cut(s) 24, 317, 578, 615, 813
MwoI GCNNNNNNNGC 2 cut(s) 317, 859
NciI CCSGG 1 cut(s) 191
NdeII GATC 6 cut(s) 235, 559, 580, 763, 1353, 1358
NlaIII CATG 3 cut(s) 176, 886, 1300
NlaIV GGNNCC 6 cut(s) 195, 386, 619, 724, 758, 962
NmuCI GTSAC 1 cut(s) 322
NsbI TGCGCA 1 cut(s) 312
PceI AGGCCT 1 cut(s) 613
PciSI GCTCTTC 1 cut(s) 869
PfeI GAWTC 4 cut(s) 1199, 1220, 1238, 1247
PfoI TCCNGGA 1 cut(s) 811
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
Psp124BI GAGCTC 1 cut(s) 82
Psp6I CCWGG 5 cut(s) 22, 315, 576, 613, 811
PspFI CCCAGC 1 cut(s) 824
PspGI CCWGG 5 cut(s) 22, 315, 576, 613, 811
PspN4I GGNNCC 6 cut(s) 195, 386, 619, 724, 758, 962
PspPI GGNCC 4 cut(s) 207, 415, 756, 815
PsrI GAACNNNNNNTAC 2 cut(s) 326, 358
RsaI GTAC 4 cut(s) 489, 494, 662, 962
RsaNI GTAC 4 cut(s) 488, 493, 661, 961
SacI GAGCTC 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 869
SaqAI TTAA 3 cut(s) 275, 449, 1347
Sau3AI GATC 6 cut(s) 235, 559, 580, 763, 1353, 1358
Sau96I GGNCC 4 cut(s) 207, 415, 756, 815
SchI GAGTC 1 cut(s) 466
ScrFI CCNGG 6 cut(s) 24, 191, 317, 578, 615, 813
SduI GDGCHC 3 cut(s) 82, 287, 637
SfaNI GCATC 2 cut(s) 112, 795
SinI GGWCC 4 cut(s) 207, 415, 756, 815
Sse9I AATT 9 cut(s) 276, 445, 672, 735, 941, 1048, 1053, 1231, 1288
SseBI AGGCCT 1 cut(s) 613
SsiI CCGC 2 cut(s) 244, 320
SspMI CTAG 3 cut(s) 609, 768, 1224
SstI GAGCTC 1 cut(s) 82
StuI AGGCCT 1 cut(s) 613
StyD4I CCNGG 6 cut(s) 22, 189, 315, 576, 613, 811
StyI CCWWGG 2 cut(s) 608, 767
TaaI ACNGT 5 cut(s) 207, 492, 930, 1135, 1174
TaiI ACGT 2 cut(s) 224, 1285
TaqI TCGA 6 cut(s) 120, 134, 281, 481, 1361, 1375
TasI AATT 9 cut(s) 276, 445, 672, 735, 941, 1048, 1053, 1231, 1288
TatI WGTACW 1 cut(s) 492
TfiI GAWTC 4 cut(s) 1199, 1220, 1238, 1247
Tru1I TTAA 3 cut(s) 275, 449, 1347
Tru9I TTAA 3 cut(s) 275, 449, 1347
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 322
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 7 cut(s) 684, 696, 731, 833, 1095, 1122, 1233
TspGWI ACGGA 2 cut(s) 59, 991
TspRI CASTG 1 cut(s) 313
VpaK11BI GGWCC 4 cut(s) 207, 415, 756, 815
XapI RAATTY 3 cut(s) 445, 672, 1048
XmaJI CCTAGG 2 cut(s) 608, 767
XmiI GTMKAC 1 cut(s) 139
XspI CTAG 3 cut(s) 609, 768, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.